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The study was to investigate the effect of Astragali Radix, Chinese yam and Crataegus pinnatifida on growth performance, hair quality, fecal characteristics, serum antioxidant, immune indexes and gut microbiota of kittens. Eighteen kittens aged 45 to 60 days were randomly divided into three groups with six in each group and one kitten in each replicate. The kittens in blank control group (group H) was fed with the basic kitten diet, the kittens in experimental control group (group R) was fed with the commercial kitten diet, and experimental group (group T) was 0.2% Astragali Radix, 0.2% Chinese yam, 0.2% Crataegus pinnatifida in the basic kitten diet. The experiment lasted for 30 d. The results showed that the average daily gain of kittens in group T was higher than that in group H and group R, and the feed to gain ratio was lower than that in group H (P>0.05). The kittens in group T fur scale thickness and the degree of adhesion were higher. The serum GSH-Px activity of group T was significantly higher than that of group R (P<0.05). The fecal score of group T was higher than that of group H and group R (P>0.05), and the intestinal flora richness was higher and the flora structure was more similar. The experiment indicates that Astragali Radix, Chinese yam and Crataegus pinnatifida can raise kittens grow performance, excrement characters, hair quality, oxidation resistance and immunity, can optimize the structure of intestinal flora, thus more conducive to the healthy growth of kittens. [ABSTRACT FROM AUTHOR]