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Fertilizer has been the ever-increasing demand in agricultural production in recent years, particularly with the escalating demand for food. Simultaneously, the low efficiency of nutrient utilization and environmental risks of fertilizers have posed substantial challenges in the world. Alternatively, polymer-coated fertilizers can play a pivotal role in nutrient utilization efficiency, thus reducing the fertilizer application rates and environmental pollution risks. This study aims to improve the membrane materials of typical polymer-coated fertilizers for the high nutrient utilization efficiency in conventional fertilizers. Polyvinyl alcohol (PVA) was also employed as the membrane substrate, in order to relieve the secondary pollution and uncontrollable nutrient release rates. Three types of membranes—PVA/CS membrane, PVA/CS/GA membrane, and PVA/CS/GA/nano SiO2 membrane—were prepared using three methods: blending with chitosan (CS), crosslinking with glutaraldehyde (GA), and incorporating nano SiO2. Additionally, pure PVA membranes were prepared for comparison. The four types of membranes were characterized using scanning electron microscopy (FE-SEM), water absorption (Q), biodegradability (De), and nutrient permeability coefficient (Ps). Results indicated that there were excellent dispersion and compatibility of nano SiO2 in the PVA/CS membrane solution. Furthermore, the biodegradation rates of the four membranes also exhibited gradual increments during the initial 21 days. Subsequently, biodegradation rates escalated rapidly from the 21st to the 56th day, followed by a slower growth rate after the 56th day. The CS was incorporated to enhance the membrane biodegradation rates, while the GA crosslinking contributed to the less membrane biodegradation in soil. However, the addition of nano SiO2 had minimally impacted the membrane biodegradation rates. Overall, all four membranes displayed favorable biodegradability, with the biodegradation rates ranging from 30% to 60% within 77 days. The water absorption of PVA/CS, PVA/CS/GA, and PVA/CS/GA/nano SiO2 membranes decreased by 43.00%, 68.79%, and 82.73%, respectively, compared with the PVA membranes. Furthermore, the nutrient permeability coefficients of PVA/CS/GA and PVA/CS/GA/nano SiO2 membranes decreased by 48.51% and 57.59%, respectively, compared with the PVA/CS membranes. Therefore, the addition of CS, GA crosslinking, and nano SiO2 all contributed to the hydrophobicity of PVA membranes. Four types of coated urea (PCU) particles—PCU-PVA, PCU-PVA/CS, PCU-PVA/CS/GA, and PCU-PVA/CS/GA/nano SiO2—were prepared, where these membrane solutions were applied to the urea particles using a drum coating machine. The nitrogen release behavior was evaluated using the soil burial. The time required for the nitrogen release of the four PCUs to reach 90% varied significantly, recorded at 5, 11, 23, and 28 days, respectively. On the 5th day, the nitrogen released from PCU-PVA was the highest at 90.64%, whereas the lowest was from PCU-PVA/CS/GA/nano SiO2 at 49.32%. The CS, GA crosslinking and nano SiO2 enhanced the membrane hydrophobicity, which in turn reduced the nitrogen release rate. The nitrogen release behavior of all four PCUs followed a first-order kinetic model, with the release rate constants (k) decreasing progressively—0.3 654, 0.2 333, 0.1 127, and 0.0 926, respectively—and correlating linearly with the nutrient permeability coefficient (Ps) of the membranes. The correlation coefficient (R²) was impressively high at 0.9 991. A series of polyvinyl alcohol/chitosan coating materials were introduced with excellent biodegradability and controllable nutrient release rates, suitable for use in polymer-coated fertilizers. These materials were effectively applied in the production of coated urea particles. A first-order kinetic model of PCU nitrogen release was established with the membrane nutrient permeability coefficient as a key parameter, in order to more efficiently and effectively guide the application of PCU. [ABSTRACT FROM AUTHOR]

Objective To explore the method of elevating and fixing the auricular cartilage scaffold to adjust the overall height of the auricle. Methods A corresponding incision was made behind the ear, and then separate and release the adhesion between the back of the auricular cartilage scaffold and the cranial wall. Several stitches were sewn at the corresponding position on the bottom of the cymba auriculae, and the cartilage scaffold was lifted and fixed on the periosteum of the cranial wall to elevate the cartilage scaffold to normal height or the same height as the contralateral side. Results Sixty-five cases of various deformities of the auricle were clinically treated after 6 to 12 months of follow-up. The height of all other patients' auricles was improved, and the height of both auricles was symmetrical except for one patient with cryptotia deformity who had a loose fixation causing the stent to move down again, three patients with cup ears and two other ear deformities due to the ear being smaller than the normal side. The lifting height of all auricles is (0.6±0.4) cm. The patient satisfaction rate is 87.7%. Conclusion This method can significantly improve the overall height of the auricle while correcting cartilage abnormalities, making the two auricles more symmetrical, and the effect is satisfactory. [ABSTRACT FROM AUTHOR]

Efficient recycling and utilization of waste heat from aluminum electrolysis cells is one of the implementation paths for the aluminum electrolysis industry to achieve deep energy conservation and carbon reduction. In response to the technical bottleneck problem of difficult recovery of waste heat on the side of aluminum electrolytic cells, a dedicated heat exchange module for waste heat recovery on the side of the electrolytic cell has been developed. The flow rate of the heat exchange medium in the module has been optimized and controlled, and the heat extraction rate has been adjusted to ensure stable operation of the electrolytic cell without affecting it. The radiant heat on the side of the electrolytic cell shell has been efficiently recovered. The technology was tested in an industrial scale on a 200 kA test cell and the results showed that the overall operation of the aluminum electrolysis cell waste heat recovery system was stable and efficient, with an average heat recovery of 77 734 kJ/h, equivalent to 21.59 kW per hour, 317 kWh per ton of aluminum, and a heat recovery efficiency of 99.71%.The system has achieved stable, efficient, and precise regulation of heat recovery within the range of 18.44-21.59 kW/h, and can optimize and regulate the furnace, which is conducive to the long-term, efficient, and stable production operation of aluminum electrolysis cells. [ABSTRACT FROM AUTHOR]

Objective To investigate the predictive value of serum plasminogen activator inhibitor-1(PAI-1) and retinol-binding protein 4(RBP4) levels for patients with acute ST-segment elevation myocardial infarction (STEMI) with‐ out reflow after percutaneous coronary intervention (PCI). Methods Totally 129 STEMI patients were selected as the STEMI group. According to the blood flow grading of thrombolysis therapy after PCI, they were divided into the no-reflow group (38 cases) and the reflow group (91 cases), and 55 healthy subjects were selected as the control group during the same period. Clinical data of STEMI patients were collected, and serum levels of PAI-1 and RBP4 were detected by en‐ zyme-linked immunosorbent assay. The predictive value of serum PAI-1 and RBP4 levels in patients with STEMI without reflow after PCI was analyzed by receiver operating characteristic (ROC) curve. Results Compared with the control group, the serum levels of PAI-1 and RBP4 in STEMI group increased (all P<0. 05). Compared with the reflow group, the serum levels of PAI-1 and RBP4 in no-reflow group increased (all P<0. 05). Multivariate Logistic regression analysis showed that the increase of age and the increase of LDL cholesterol, PAI-1 and RBP4 were independent risk factors for noreflow after PCI in STEMI patients (all P<0. 05), and the increase of left ventricular ejection fraction was a protective fac‐ tor (P<0. 05). ROC curve analysis showed that the area under the curve of serum PAI-1 and RBP4 levels was greater than that of PAI-1 and RBP4 alone in predicting no-reflow of STEMI patients after PCI (both P<0. 05). Conclusion The in‐ creased serum levels of PAI-1 and RBP4 in STEMI patients are associated with no-reflow after PCI, and the serum PAI-1 combined with RBP4 has a high value in predicting no-reflow after PCI. [ABSTRACT FROM AUTHOR]

Objective To observe the short-term efficacy of domestic RECO stent used in endovascular treatment of acute posterior circulation infarction, and to analyze the factors affecting the prognosis of patients. Methods The clinical data of 148 patients with acute posterior circulatory infarction who used domestic RECO stents and Solitaire stents for endovascular treatment admitted to The First Department of Neurosurgery, Tieling Central Hospital from December 2018 to October 2020 were analyzed retrospectively. The recanalization of the blood vessels in patients with two different thrombectomy stents were observed, and the mRS score of patients after 3 months were counted. After 3 months, patients with mRS scores of 0-2 were classified as the good prognosis group, and patients with mRS scores of 3-6 were classified as the poor prognosis group. The general clinical data of the two groups of patients with good and poor prognosis using domestic RECO stents were compared. The relevant factors that may affect the prognosis of patients were analyzed. And the clinical effect of using other stents in the same period for emergency postertor-circulation embolectomy were compared. Results Among 98 patients with acute posteriorcirculatory infarction using domestic RECO embolization stent, 90 were recanalized and 8 were not recanalized. After 3 months, the mRS score showed good prognosis group was 60 cases, and poor prognosis group was 38 cases. There were 50 patients with acute posterior circulation infarction, 45 patients with recanalization, 5 patients without recanalization, 3 patients with postoperative death, 31 patients with good prognosis and 19 patients with poor prognosis after 3 months of mRS score. There was no significant difference in the short-term efficacy of domestic RECO stent used in the treatment of acute posterior circulation infarction compared with Solitaire stent (P ＞ 0. 05). After analyzing the related factors that may lead to poor prognosis of patients who use domestic RECO stents to remove thrombi,it is concluded that PC-ASPECTS score at admission,collateral compensation,NIHSS score at admission,time from onset to femoral artery puncture,times of thrombus removal,and whether diabetes are associated with the related factors that affect the prognosis of patients. Conclusions The domestic RECO stent used in the endovascular treatment of acute posterior circulation infarction has a significant short-term effect. The higher the patient's PC-ASPECTS score,the better the collateral compensation,the lower the NIHSS score at admission,and the shorter the time from onset to femoral artery puncture. The near-term prognosis is better. [ABSTRACT FROM AUTHOR]

The discordant position of theoretical engagement point and actual engagement point was due to the tooth profile deviation of cycloid gear. Aiming at this problem, a method to determine the real engagement point of cycloid-pin pair with tooth profile deviation was proposed. Firstly, the cycloid tooth surface was sampled by the gear measuring center, and the digital representation of the tooth profile was obtained based on cubic B-spline curve reconstruction. Then, based on the cycloid-pin pair transmission principle and gear train conversion principle, it was transformed into fixed axle gear train and the real contact analysis model of cycloid-pin pair was established. A method to solve the model was proposed and the results were calculated by MATLAB programming, and then the contact impressions were obtained. Finally, the meshing point positions and imprints of cycloid-pin pair obtained from theoretical standard tooth profile and actual tooth profile were compared and analyzed. The research results show that, the cycloid rotation angle at the real engagement point of cycloid-pin pair is 0.3° different from that at the theoretical engagement point, the tooth profile deviation has an effect on the meshing point position of cycloid-pin pair, which provides support for the research of the actual meshing characteristics of cycloid-pin pair. [ABSTRACT FROM AUTHOR]

Rejection has constantly been an unresolved challenge in the field of organ transplantation. The research on the mechanism of rejection plays a significant role in improving the efficacy of organ transplantation and enhancing the survival rate of graft. The innate and specific immune responses of the human body jointly participate in the graft rejection, leading to graft injury. In recent years, multiple researchers have conducted in-depth studies on the mechanism underlying the role of microRNA (miR) in regulating rejection. Among them, miR-155 has been widely considered as a key factor involved in immune regulation. The expression level and functional status of miR-155 may be intimately associated with the occurrence of rejection, which may become a new target for overcoming rejection. In this article, relevant studies on the role of miR-155 in regulating key immune cells in innate and specific immune responses were reviewed, aiming to provide novel ideas for the development of new immunosuppressants and rejection therapy. [ABSTRACT FROM AUTHOR]

Objective To assess the relationship between diabetes mellitus and risk of anterior circulation aneurysm rupture. Methods The clinical data of 680 patients with intracranial aneurysms (IA) were analyzed retrospectively. The correlation between diabetes and the risk of rupture of IA was further judged by univariate and multivariate analysis. Results In univariate analysis, hypertension (P=0.001), family history of IAs (P = 0.007), history of subarachnoid hemorrhage (P = 0.039), irregularity (P = 0.001), size≥8 mm (P = 0.006) and diabetes (P=0.005) might have statistical differences. After excluding the influence of confounding factors, the results of multivariate analysis showed that the proportion of diabetes was statistically different between the ruptured group and the unruptured group (OR = 1.68,95% CI: 1. 160~2.470, P = 0.025). Conclusion Diabetes may increase the risk of rupture of anterior IA, which may be related to the metabolic disorder related angiopathy caused by diabetes and the mutation of diabetes related genes (SLC2A9, TOX), etc. [ABSTRACT FROM AUTHOR]

Representation learning of a user-item interaction network becomes an effective recommendation method. Most of the existing methods regard the interaction network as a static homogeneous network, ignoring the impact of interaction timing and node heterogeneity. In response to this problem, this paper proposed a recommendation method based on dynamic bipartite network representation learning. Firstly, the method constructed a time-series weighted bipartite network, and then respectively mapped user nodes and item nodes to different vector spaces to preserve the heterogeneity of the network, and aggregated the first-order and high-order neighbor information for center nodes with graph convolution. Finally it used a multi-layer perceptron to learn the nonlinear relationship between the two types of node embeddings and performed top-N recommendation. Experimental results on Amazon and Taobao datasets show that the proposed method is significantly superior than the related methods based on static or heterogeneous network representation learning in HR and NDCG indicators. [ABSTRACT FROM AUTHOR]

Objective To investigate the effects and mechanism of Taoren Honghua decoction on the gestational diabetes mellitus（GDM）rats and the outcomes of their offspring. Methods Forty SPF SD female rats and 80 SPF SD male rats were selected. Forty female rats were randomly divided into groups A，B，C and D，with 10 rats in each. During the estrous period，female and male rats were caged at the ratio of 1：2，and the pregnancy of female rats was confirmed by vaginal smear method. On the 5th day of pregnancy，rats in the group B，group C and group D were injected with STZ in caudal vein to prepare GDM model. Rats in the group A were injected with equal volume of citrate-disodium hydrogen phosphate buffer，rats in the group C were given Taoren Honghua decoction 10 g/kg intragastrically on the modeling day，rats in the group D were given metformin 200 mg/kg intragastrically；rats in the groups A and B were given the same volume of normal saline，once a day，for 19 days. Fasting blood glucose（FPG）and insulin were measured by ELISA before pregnancy and on day 7 and 19 of pregnancy，respectively. After anesthesia on day 19 of gestation，the embryos were extracted and separated by laparotomy. The number of embryos in each group was calculated，and the amount of fetal body fat at birth was measured. The pathological changes of placental tissues were observed by HE staining，and the mRNA expression of insulin-like growth factor 2mRNA binding protein 2（IGF2BP2）and nuclear factor κB（NF-κB）in the placental tissues were detected by qRT-PCR. The protein expression levels of IGF2BP2 and NF-κB in placental tissues were detected by Western blotting. Results On day 7 and 19 of pregnancy，the levels of FPG and insulin in the group B were higher than those in the group A（all P<0. 05），and the above indexes in the group C were lower than those in group B（all P< 0. 05）. Compared with the group A，group B had lower embryo survival and birth body fat（both P<0. 05）. Compared with the group B，group C had higher embryo survival and birth body fat（both P<0. 05）. The placental tissues of group A were normal. In the group B，the placental tissue layer was blurred，the intercellular space was enlarged，and the cell layout was disordered. The pathological changes of placenta tissues in the groups C and D were lighter than those in the group B. Compared with the group A，the mRNA and protein expression levels of IGF2BP2 and NF- κB in the group B were higher（all P<0. 05）. Compared with the group B，the expression of the above indexes in group C was lower（all P< 0. 05）. There were no statistically significant differences in the above indexes between group C and group D（all P> 0. 05）. Conclusions Taoren Honghua decoction can reduce blood glucose and insulin levels，increase the survival rate and body fat amount of offspring embryos，and improve placental tissue in GDM rats during pregnancy. The mechanism may be related to inhibiting the activation of IGF2BP2 and NF-κB signaling pathways. [ABSTRACT FROM AUTHOR]

本文分析1例重症肌无力患者的心电图及心肌酶改变,发病过程中心电图酷似急性 心肌梗死,但经免疫吸附､丙种球蛋白等对症治疗后,心电图迅速恢复正常。考虑本例并非急 性冠脉病变,而是由重症肌无力抗心肌抗体的出现所致的心肌一过性损害。 [ABSTRACT FROM AUTHOR]

Objective To investigate the effects of isoalantolactone on proliferation and apoptosis of human bladder cancerT2 4 cells and its mechanism. Methods CDT2 4 cells in the logarithmic phase were divided into the groups 1, 2, 3, 4, 5 and 6; cells in the groups 1-5 (experimental group) were added with different concentrations of isoalantolactone (5, 10, 20, 40, and 80 μmoVL), and group 6 with normal culture medium (control group) ; after 24, 48, 72 h of culture, the cell proliferation ab山ty of each group was detected by C C K-8 method. (2) T24 cells were divided into the groups A, B, C, D, and E; cells in the group A were cultured in medium without isoalantolactone, cells in the groups B and E were pretreated with 5 nmol/L N-acetylcysteine (NAC ) for 2 h, and cells in the groups C, D and E were cultured with 10, 20 and 20 μmol/L isoalantolactone; after 24 hours of culture, the apoptosis rate of each group was measured by flow cytometry, the level of reactive oxygen species (ROS) was detected by DC FH-DA, and the mitochondrial membrane potential was detected by JC-1 mitochondrial membrane potential. @ T24 cells were divided into 4 groups with 6 compound wells in each group; cells in the three groups were added with 10, 20 and 40 μ,moVL isoalantolactone, and cells in the other group were treated with culture medium without isoalantolactone (control group) ; after 24 hours of culture, Western blotting was used to detect apoptosis-related proteins Bax, B-lymphocyticoma-2 (Bcl-2), Cytochrome C, and Caspase-3. Results (D Compared with the group 1, the inh山山on rate of cell proliferation increased in the groups 2, 3, 4 and 5 (all P < 0. 05) ; compared with the group 2, the inhibition rate of cell proliferation increased in the groups 3, 4 and 5 (all P < 0. 05) ; compared with the group 3, the inh如tion rate of cell proliferation increased in the groups 4 and 5 (all P < 0. 05). @ Compared with the group A, the apoptosis rate and ROS content increased in the groups C and D (all P < 0. 05) ; compared with the group C, the apoptosis rate and ROS content increased in the group D (all P < 0. 05) ; compared with the group D, the apoptosis rate and ROS content were reduced in the group E (all P < 0. 05). Compared with the group A, the mitochondrial membrane potential of group C decreased (P < 0. 05). Compared with the group C, the mitochondrial membrane potential of group D decreased (P < 0. 05) ; compared with the group D, the cell mitochondrial membrane potential increased in the group E (P < 0. 05). @ Compared with the control group, the relative expression levels of Bax, Cytochrome C and Caspase-3 in the drug groups increased, and the relative expression levels of Bcl-2 decreased (all P <0 . 05); with the increase of concentration, the relative expression levels of Bax, Cytochrome C and Caspase-3 in the drug group increased, and the relative expression levels of Bcl-2 decreased (all P <0. 05). Co nclusion lsoala ntolactone can inhibit cell proliferation and induce apoptosis by promoting the accumulation of reactive oxygen specie, decreasing the mitochondrial membrane potential and regulating the expression of apoptosis-related proteins [ABSTRACT FROM AUTHOR]

Objective To explore the application of totally expanded skin for auricular reconstruction in patients with grade III microtia. To reconstruct tragus and concha cavity by rational utilization of remnant ear. Methods There were 36 patients suffered from microtia with remnant ear in the new method group. In the first stage of operation, a tissue expander of 150 ml was implanted to the dual- plane of postauricular mastoid region through the incision of remnant ear. In the second stage, autologous costal cartilage framework was implanted for auricular reconstruction. In the third stage, the skin of remnant ear was designed to reconstruct the tragus and cover partial wounds of the concha cavity. The cartilage of remnant ear was used as the internal support of tragus or inserted to the bottom to increase the height of framework. There were 46 patients in the traditional expansion group. In the third stage of operation, the remnant earlobe was transferred to reconstruct earlobe. The tragus was reconstructed by M flap. The free skin graft was applied for concha cavity. All patients were followed up from 6 to 12 months, the size of tragus and concha cavity and satisfaction of patients were evaluated. Results No obvious scar was found on the surface of reconstructed auricle in the new method group. A blister of 0.6 cm × 0.4 cm at distal remnant earlobe flap was found in 1 patient and cured after dressing change of 10 days. In the traditional expansion group, blister was found in skin graft of concha cavity in 5 patients and cured after dressing change. During the follow-up, the contracture and deformity of tragus and retraction rate of concha cavity was less than that of the traditional expansion group. The patients' satisfaction of the new method group was significantly higher than that of the traditional expansion group (P<0.05). The fullness of reconstructed earlobes was slightly worse than normal. ConIIIclusion Enough skin could be expanded to totally covered the surface of the whole cartilage framework. There was no need for earlobe transposition. No surgical scar was found on the framework surface of the reconstructed ear. The remnant ear could be adequately used. The appearance of tragus and concha cavity of reconstructed ear was good. [ABSTRACT FROM AUTHOR]

Cervical diseases include cervical inflammation, cervical squamous intraepithelial lesions and cervical cancer. In recent years, it has been found that vaginal microflora, its cytokines and local immune system are related to the occurrence and development of cervical diseases. The vaginal micro-ecological environment of normal women is composed of a variety of microorganisms, of which lactobacillus play an important role in maintaining vaginal micro-ecological balance. The incidence rate of cervical cancer ranks first in female malignant tumors, and its occurrence is closely related to persistent infection of human papilloma virus. However, not all people infected with human papilloma virus develop into cervical cancer. Cervical cancer is caused by multiple factors. Vaginal diseases caused by changes in lactobacillus interact with human papilloma virus infection, and both participate in the occurrence and progression of cervical diseases. Systematic effective vaginal micro-ecological environment testing is very important for timely regulation of vaginal micro-ecological balance. The relationship between vaginal microflora and cervical diseases has become a research hotspot of many scholars. This article explores the occurrence and development of vaginal microecology and cervical diseases, and provides a theoretical basis for the prevention treatment and prognosis of cervical diseases, especially cervical cancer. [ABSTRACT FROM AUTHOR]

Objective To observe the neuroprotective effect of herbal medicine Gengnianchun formula (GNC) on primary cultured rat fetal hippocampal neurons injury induced by amyloid beta 25-35 fragment (Aβ25-35)through ERβ and PI3K/Akt signaling pathway. Methods Human neuroblastoma cell line (SH-SY5Y) were treated with Aβ25-35 to establish an in vitro cell model of Alzheimer's disease (AD) and were cultured with different concentration of GNC serum (GS) and Aβ25-35 to determine the appropriate treatment concentration and duration of these drugs by cell viability test (CCK-8) method. Primary cultured rat fetal hippocampal neurons were treated with Aβ25-35 to establish an in vitro cell model of AD and were divided into 5 groups,including control group,model group,GS group,normal saline serum(NS) group and PHTPP(a selective ERβ antagonist) group. Morphological observation,CCK-8 test and LDH release assay were used to evaluate the cell injury of primary cultured rat fetal hippocampal neurons. Hoest33258/PI double fluorescence staining,qRT-PCT and Western blot experiments were used to evaluate the cell apoptosis of primary cultured rat fetal hippocampal neurons. Results Compared with the control group,synaptic shrink,collapse and cell debris of primary cultured rat fetal hippocampal neurons were more obvious,cell viability decreased and LDH release increased in model group. Compared with the model group and NS group,synaptic shrink,collapse and cell debris of primary cultured rat fetal hippocampal neurons reduced,cell viability increased,LDH release decreased,number of PI positive primary cultured rat fetal hippocampal neurons were decreased,levels of bax/bcl-2 and cyt-c mRNA and the protein levels of bax/bcl-2,cleaved-caspase-3 and cyt-c were down-regulated and level of p-Akt was up-regulated in GS group. Compared with GS group,levels of bax/bcl-2 and cyt-c mRNA and the protein levels of bax/bcl-2,cleaved-caspase-3 and cyt-c were up-regulated and level of p-Akt was down-regulated in PHTPP group.Conclusions Neuroprotective effect of GNC on primary cultured rat fetal hippocampal neurons injury was mediated by or at least partly by ERβ and PI3K/Akt signaling pathway. [ABSTRACT FROM AUTHOR]

Objective To explore the Rap 1/PI3K/AKT signaling pathway in inducing apoptosis of mouse spermatocytes (GC 一 2 spd) induced by tris (1,3-dichloro -2 - propyl) phosphate (TDCIPP) the role of death. Methods GC - 2 spd cells were treated with 0, 15, 30, and 60(xmol/L TDCIPP for 24 hours. Using flow cytometry to detect cell expression levels, Western blotting to examine the expression levels of Rapl, PI3K, AKT, phosphorylated AKT (p - AKT) proteins, as well as related proteins cleared PARP and cleared Caspase - 3 in cells, and apoptosis - related proteins cleaved PARP and cleaved Caspase - 3 in the cells. ESI09 was used to inhibit the expression of Rapl protein in GC -- 2 spd cells, followed by exposure to TDCIPP. Western blotting and flow cytometry were used to respectively assess the protein levels of PI3K, AKT, p 一 AKT, cleaved PARP, and cleaved Caspase - 3, as well as the level of cell apoptosis. Results Compared to the control group, the cell apoptosis and expression of cleaved PARP and cleaved Caspase 一 3 proteins were significantly increased in the TDCIPP 一 exposed cells (P < 0. 01), The expression level of Rapl was significantly increased (P < 0. 01), while the expression levels of PI3K, AKT, and p - AKT were decreased (P <0. 01). Compared to the TDCIPP 一 exposed group, the expression of Rapl was down - regulated (P < 0. 01), while the expression levels of PI3K, AKT, and p 一 AKT proteins were up 一 regulated (P < 0.05) in the cells co 一 treated with ESI09 and TDCIPP. Meanwhile, the expression levels of cleaved PARP and cleaved Caspase -3 in the cells, as well as the level of cell apoptosis, were significantly reduced (P < 0. 05). Conclusion TDCIPP induces overexpression of Rapl protein and obstructs PI3K/AKT signaling transduction, eventually leading to apoptosis of mouse spermatocytes. [ABSTRACT FROM AUTHOR]

目的 探讨局部皮瓣联合耳前带蒂组织移植矫治耳垂发育不良的临床效果。方法 自 2008 年 1 月至 2020 年 1 月，华中科技大学同济医学院附属协和医院整形外科对 8 例先天性耳垂发育不良患者进行手术治疗，在耳垂下方设计切口， 形成一皮瓣，局部旋转缝合，以增加耳垂的皮肤；再于耳前形成一蒂在耳垂前的真皮脂肪瓣，将之插入耳垂前外侧与后内侧皮肤 之间分离形成的腔隙内，缝合固定，缝合供瓣区切口。结果 所有患者的切口均一期愈合，无皮瓣坏死发生。1 例患者出现切口 瘢痕增生，经外用药物涂抹等治疗后瘢痕软化。所有患者仅经 1 次手术治疗；获随访 6~18 个月，无脂肪液化、感染、硬结、 皮下包 块等并发症发生。手术后耳垂形状正常，较术前明显饱满，患者对耳垂外观满意度较高。结论 采用自体局部皮瓣联合带蒂真皮 脂肪组织转移修复，可明显改善耳垂外观，效果稳定持久。 [ABSTRACT FROM AUTHOR]

Cellulose nanocrystallirie/Fes 0i nanospheres were iri-situ synthesized with cellulose nanocrystalline (CNC). The particle size？ crystallization？ magnetic properties and electrochemical properties of CNC/Fe.s0-i nanospheres were analyzed. The results show that CNC/F:> O-i nanospheres were uniform in size with a diameter of 1 0-30 nm. The magnetization of CNC/Fe.sO-i rianospheres was 34.9 emu/g. The specific capacitance of the CNC/Fe 0.•! was mainly the tantalum capacitor generated by Fe").卜 The CNC/Fe.sO-i nanospheres showed a specific capacitance of 30.14 F / g at 0.03 A / g in 1 mol / LNa S0; aqueous solution. The specific capacitance retention was about 78.76% at 0.04 A / g after 500 cycles. The diffusion of ions in the CNC / Fe") i was the Warburg mechanism. [ABSTRACT FROM AUTHOR]

[Objective] MEP pathway is one of the important ways to synthesize alkaloids and terpenoid precursors, and 1 -deoxy-D-xylulose-5-phosphate synthase (DXS) is the first pivotal enzyme in the MEP pathway. In order to understand the structure and function of Fritillaria DXS, molecular biology were used to analyze it, which established a foundation for the synthesis of Fritillaria alkaloids. [Method] The open reading frame (ORF) sequence of FcDXS gene was cloned by PCR and sequenced by bioinformatics to predict its potential function. The expressions of FcDXS gene in the wild (root, stem, leaf, bulb) and tissue culture inducer (callus) were measured by qRT-PCR. [Result] The 2151 bp ORF fragment of FcDXS gene was obtained and encoded a polypeptide of 716 amino acids with a similarity of more than 80 % to the plant DXS protein such as Amomum villosum published by NCBI. In the secondary and tertiary structure prediction of FcDXS protein, it was concluded that the FcDXS protein consists mainly of a-helix; The results of qRT-PCR showed that the expression of FcDXS gene in the wild was the highest, and the expression in hormone-induced callus was 5 times that in the wild bulb. [Conclusion] Bioinformatics analysis of FcDXS protein features and homology showed that the protein was conserved domain, combining with fluorescence quantitative analysis results proved that FcDXS may be a biologically functional and hormone-induced expression of the protein. This study established a theoretical foundation for the subsequent use of genetic engineering to improve the content of alkaloids of Fritillaria cirrhosa. [ABSTRACT FROM AUTHOR]

[Objective] To study the effects of soil conditioners on the improvement of heavily cadmium contaminated soil and the absorption and accumulation of cadmium by crops. Method] The surface soil of Enshi area rich in selenium and high cadmium was used as the pot experiment soil, and pepper was used as the test crop. Through pot experiment, the effects of nine soil conditioners on the growth, absorption and accumulation of cadmium in pepper, as well as the change of cadmium morphology in soil were studied. (Result] The application of soil conditioners could significantly reduce the content of cadmium in pepper fruits by 76.35%-90.27%, and T4 soil conditioner (12.99% wheat straw biochar +6.49% superphosphate +64.94% attapulgite +2.60% silicon fertilizer +12.99% shell powder) had the best effect overall. Compared with rapeseed dregs, biochar in experimental group had a significant effect on soil pH. The application of soil conditioner could significantly reduce the content of exchangeable and carbonate-bound cadmium, and promote the conversion of cadmium to the unusable iron-manganese binding state and residue state, and increase the photosynthetic rate of plant leaves and promote plant growth. The correlation between the content of cadmium in pepper fruit and cadmium in soil indicated that pepper could absorb both water-soluble cadmium and carbonate-bound cadmium in soil. [Conclusion] The combination of "12.99% wheat straw biochar +6.49% superphosphate +64.94% attapulgite +2,60% silicon fertilizer + 12.99% shell powder" can effectively reduce the uptake of cadmium by crops in selenium-rich and cadmium-high areas of Enshi. The safe planting of crops in high cadmium area should not only reduce the activities of water-soluble and ion-exchange cadmium, which can be directly used by plants, but also reduce the content of carbonate-bound cadmium, which has potential risks. [ABSTRACT FROM AUTHOR]

目的观察小儿妓翘清热颗粒联合重组人干扰素alb 治疗小儿急性上呼吸道感染( AURI) 的临床疗效。 方法选取2018 年1 月至2019 年3 月该院收治的AURI 患儿122 例作为研究对象， 按照治疗方案的不同将其分为对照组和治 疗组，每组各61 例。对照组仅子以重组人干扰素alb 注射液雾化吸入治疗， 冶疗组在对照组基础上加用小儿妓翘清热颖杠冶疗， 2 组均连续治疗1 周。比较2 组临床疗效， 以及治疗前后血清促炎因子、抑炎因子水平。结果治疗后， 治疗组血清肿瘤坏死因 子(TNF) a、白细胞介素4(IL 4) 、IL 6、IL 8、IL 10 水平均明显低于对照组， 而IL 2 水平则高于对照组， 差异均有统计学意义 (P<0. 05) 。治疗组临床疗效明显高于对照组， 差异有统计学意义(P<0. 05) 。结论小儿妓翘清热颖粒联合重组人干扰素alb 可有效改善AURI 患儿机体炎症状态， 疗效显著，值得临床推广。 [ABSTRACT FROM AUTHOR]

The treatment of premenopausal patients with endometrial cancer has not yet been standardized, first of all, drug dosage, drug intake, treatment time and so on, there are no clear standards. Secondly, because of the high recurrence rate, is it unclear whether the patient needs to be removed after delivery. By reading a large number of literature and clinical prospective study, the main drug treatment is given priority to with low dose, treatment time is generally about 9-12 months. In addition to the traditional simple oral hormone therapy, in recent years, the treatment of LNG-IUS, the LNG-IUS and GnRHa combined therapy, hysteroscopic surgery and hormone combination therapy are gradually carried out. And through assisted reproductive technology, the success rate of pregnancy is increasing after treatment. Research shows that the treatment of premenopausal endometrial cancer is very promising at present, but a large number of clinical trials are needed to improve the treatment strategy. In this paper, by combining with clinical practice and the literature, we do a review about indication selection, pretreatment evaluation, treatment plan, curative effect evaluation, condition monitoring, and the problems that need to be faced after birth. [ABSTRACT FROM AUTHOR]

Sclerostin,a protein encoded by sclerosteosis( SOST) gene,antagonizes the Wnt protein via the low density lipoprotein receptor-related protein( LRP5/6) receptor and inhibits bone formation. It has been a new target for the treatment of osteoporosis.Both basic and clinical researches have indicated that sclerostin monoclonal antibody can treat osteoporosis by increasing bone mineral density,promoting bone formation and inhibiting bone resorption. However,limited data have evaluated the adverse effects of sclerostin monoclonal antibody and its influence on bone fracture, which deserve further studies. [ABSTRACT FROM AUTHOR]

Pre -eclampsia is a pregnancy complication with multiple organs and system injuries, seriously impairing maternal and fetal health. Nevertheless, the etiopathogenesis of pre -eclampsia has not been fully elucidated yet. In normal pregnancy, Toll-like receptor 4 (TLR4) at the maternal-fetal interface regulates the homeostasis of the inflammatory-immune microenvironment. In pre -eclampsia, abnormal TLR4 signaling at the maternal -fetal interface not only mediates deviated inflammatory-immune reaction, but also interacts with other novel pathological phenomena, including ferroptosis, pyroptosis, metabolism reprogramming, and cellular senescence. Furthermore, TLR4 at the maternal -fetal interface may mediate the abnormal development of the nervous system in the offspring. In addition, targeting TLR4 at the maternal-fetal interface may be one of the potential pharmacological mechanisms for new therapeutic research in pre -eclampsia. Based on recent relevant literature, this article explores the multiple possible roles of TLR4 at the maternal-fetal interface in pre-eclampsia and provides reference for the study of the pathogenesis and therapies of pre-eclampsia. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of Artesunate (Art) on the expression of transferrin receptor (TtR)in K562/ADM cells. Methods: The drug-resistant K562/ADM cells were cultured with 1000 ng/mL doxorubicin for two weeks followed by Artesunate treatment with different concentrations (12.5 μg/mL, 25μg/mL and 50 μg/mL) or different time (12 h, 24 h, 36 h, and 48 h). The content of transferrin receptor in K562/ADM cells was determined by flow cytometry. The effect of Artesunate on the expression of transferrin receptor protein in K562/ADM cells was measured by Westem blot. Cell counting kit-8 (CCK-8) assay was used to evaluate inhibitory effect of Art combined with doxorubicin (ADM) in K562/ADM cells.The reversal index was defined as the IC50 of the experimental group divided by the IC50 of the control group in K562/ADM cells. Results: Art effectively decreased the content of transferrin receptor and the expression of transferrin receptor protein in K562/ADM cells in a dose-dependent manner. Moreover, Art also inhibited transferrin receptor protein expression in K562/ADM cells in a time-dependent manner.The different concentrations of Art(12.5 μg/mL, 25μg/mL and 50 μg/mL) could induce reversal of drug-resistance with the reversal index being 1.38, 2.12 and 2.95 times (P＜0.05). Art inhibited cell proliferation of K562/ADM cells, and the IC50 were19.7 μmol/L. Conclusions: Art effectively down-regulated the transferrin receptor content as well as transferrin receptor protein expression in K562/ADM cells, which resulted in reversal of drug resistance of K562/ADM cells. Art also inhibited K562/ADM cells proliferation, which has great value in clinical treatment of leukemia. [ABSTRACT FROM AUTHOR]

In order to study the mechanical behavior of reinforced concrete columns strengthened with high ductile concrete (HDC), eight reinforced concrete columns were designed and strengthened with HDC jacket and high performance composite mortar jacket. The failure modes, deformation capacity and energy dissipation capacity of the specimens were studied by low cyclic loading tests. The test results show that HDC jacket could provide good constraining at the core concrete. The failure mode is changed from brittle failure to ductile failure, and the bearing capacity, ductility and energy dissipation capacity of the strengthened columns are significantly improved after strengthening. The good synergic work performance between HDC jacket and concrete could take advantage of the high damage tolerance capacity of HDC, and the HDC jacket is much more effective than the mesh reinforced high performance composite mortar in behavior enhancement of the columns. Configuring mesh reinforcements in the HDC jacket could improve the constraining effect of the jacket on the inner concrete, and further improve the ductility and energy dissipation capacity of the strengthened columns, but contribute little to the bearing capacity of the strengthened columns. Using HDC jacket to strengthen concrete columns could reduce the damage degree of the vertical components and improve the seismic behavior of the components. [ABSTRACT FROM AUTHOR]

Objective: To establish SRB1 gene-knockdown microglia cell line and investigate the function of microglia SRB1 receptor in the phagocytosis of Aβ during the process of Alzheimer's disease (AD). Methods: The expression of SRB1 gene in human microglia 1900 was determined by reverse transcription-PCR. three lentiviral sliRNA targeting SRB1 were designed and the best interferring sliRNA sequence was selected. The resulting lentiviral construct was used to knockdown SRB1 gene in human microglia. Intracellular Aβ42 Quantification was performed to test the phagocytosis function of the SRB1 knockdown microglia cell line. Results: The lentiviral particles were successfully prepared and showed effective infection capacity and knockdown effects. SRB1 gene silencing was performed in Human microglia 1900 with a knock-down efficiency of 83.7%. The phagocytosis function was significantly decreased to 57% in SRB1 gene-silencing microglia cell line. Conclusion: The SRB1 knock-down cell line was established. The function of SRB1 gene was confirmed by oligomer Aβ(oAβ) phagocytosis test. These findings have identified a significant role of SRB1 in Aβ clearance. [ABSTRACT FROM AUTHOR]

With the development of IT technology，internet information resources become much ric­her. We can obtain relevant knowledge from complicated internet information thanks to the rapid devel­opment of big data technology. The most essential part is the big data crawler technology which can crawl and save Internet data structurally. In this paper，we present and develop an efficient Deep Web information crawler based on Hadoop.This crawler employs the Webkit as the core engine which can implement the visual configuration and the deep data collection. To improve the efficiency，the data collection algorithm is also optimized by adjusting the strategy of task distribution in Hadoop. Experimen­tal results demonstrate that the developed data collection platform can obtain better results. [ABSTRACT FROM AUTHOR]

[Objectives] In our previous study, two HrpZ proteins, HrpZpSgS1 and HrpZpsgA1, were identified from Pseudomonas syringae pv. glycinea A1 and S1 strains, and the HR elicitation activity of HrpZpsgs1 in tobacco was stronger than that of HrpZp5gA1- Compara­tive analysis of their amino sequences showed that three different regions mainly existed in C-terminal. In order to clarify the relation­ship of these differential regions of HrpZ proteins and the ability in eliciting the HR and disease resistance，we constructed 6 recom­binant proteins by respectively exchanging three differential regions between HrpZpsgs1 and HrpZp5gA1. [ Methods] After expression and purification，6 recombinant proteins about 41 x 103 were got，and HR elicition ability and Tobacco Mosaic Virus ( TMV) disease resistance ability were detected. [ Results] HrpZs(s2→A2) and HrpZA(A3→s3) could induce stronger HR in tobacco compared with the wild-type proteins，furthermore，all of 6 recombinant proteins could reduce the phenotype caused by inoculation with TMV better than wild-type proteins，and among them HrpZA(A3→s3) and HrpZs(s3-A3) showed the best effects. [ Conclutions] These results showed that the 7,8,9 a-helices were the main regulatory regions of HR，and thse 8,9 a-helices were also related to the disease resistance. This study provides the theoretical basis for molecular modification of HrpZ harpin protein in the future. [ABSTRACT FROM AUTHOR]

Poor ovarian response (POR), which means insensitive to gonadotrophin in the controlled ovarian hyperstimulation (COH), can result in fewer retrieved oocytes, higher cycle cancel rate and reduced clinical pregnancy rate. About 9%-24% of women who received COH treatment could have POR, which is one of the most challenging tasks in reproductive medicine. Until now, there is not accordant diagnostic criteria of POR. In 2011, ESHRE (European Society of human Reproction and Embryology) reached the Bologna Criteria:①Age over or equal to 40 years, or other high-risk factor;②History of POR (the number of retrieved oocytes in routine COH was less than or equal to 3); ③The lowered ovarian function, such as AFC〈5-7 or AMH〈0.5-1.1 ng/mL. POR can be diagnosed if there are two in above three criterions. In this paper, we also introduced the treatment progress of POR, such as the adjusting COH, the increasing dose of gonadotrophin in COH, the pretreatment with androgens and oral contraceptive pills, and the adding growth hormone and aspirin. [ABSTRACT FROM AUTHOR]

目的 探讨保守性手术治疗不同类型子宫内膜异位症（EMT）合并不孕的疗效。 方法 对该院2009年3月至2013年8月收治的行保守性手术治疗的不同类型EMT合并不孕患者的临床资料进行回顾性分析。 结果 180例EMT合并不孕患者中，妊娠66例，妊娠率为36.67%。其中腹膜型组与内囊型组妊娠率（42.62%、38.78%）均显著高于腺肌病型组（9.52%），差异有统计学意义（P<0.05）；腹膜型组内使用促性腺激素释放激素激动剂（GnRH-a）治疗后，妊娠率（61.54%）显著高于未用药组（24.24%），差异有统计学意义（P<0.01）。 结论 保守性手术可改善EMT合并不孕患者的妊娠率，尤其是腹膜型与内囊型EMT，而加用GnRH-a可显著提高腹膜型EMT患者的妊娠率。 [ABSTRACT FROM AUTHOR]

Aiming at the problem that MANET routing protocols such as DSDV, AODV, ZRP, LAR cause the surge of routing overhead of the whole network with the increase of the number and scale of nodes, a MANET Routing Strategy Based on link state monitoring is designed and proposed. The strategy is from the point of view by reducing the redundancy of routing overhead information and accurate feedback of link state. Firstly, the monitoring node is selected based on the monitoring capability value, and then the monitoring node monitors the link state of the whole network through the monitoring link to accurately get the topology information of the whole network. Then, the remaining nodes of the whole network can query the routing information to the target node from the monitoring node. Simulation experiment shows that the effectiveness and feasibility of the routing strategy. The results show that when the number of nodes in MANET is large, the routing strategy can significantly reduce the routing overhead of MANET compared with traditional routing protocols. [ABSTRACT FROM AUTHOR]

Tacrolimus (Tac) is a commonly used immunosuppressant after organ transplantation, which has high immunosuppressive efficacy. However, the pharmacokinetics of Tac significantly differ among individuals, and gene polymorphism is the main influencing factor. In recent years, the gene polymorphism of drug transporter has become a novel research hotspot. Nevertheless, the effect of the gene polymorphism of transporter on Tac pharmacokinetics remains controversial. Consequently, the correlation between the gene polymorphism of transporter and Tac blood concentration plays a significant role in guiding Tac-based individualized immunosuppressive therapy. In this article, the research progresses on the gene polymorphism of adenosine triphosphate-binding cassette (ABC) transporter and solute carrier (SLC) transporter in organ transplantation was reviewed. The correlation between the gene polymorphism of transporter and Tac blood concentration was summarized, aiming to provide reference for Tac-based individualized therapy. [ABSTRACT FROM AUTHOR]

In order to ensure the rational use of selenium fertilizer and the safe production of selenium rich rice, experiments were designed to explore the response of selenium, other mineral elements and heavy metals in rice plants to exogenous selenium. The rice variety 'Ezhong No. 5' and selenium fertilizer 'Zhongdixineng' were taken as the experimental objects, and the field randomized block design was adopted. Four selenium fertilizer concentrations (0, 15, 30, 60 g/hm²) were set for treatment. The selenium content in organs of paddy rice plants, different mineral elements in brown rice, and different heavy metal elements in leaves were measured at the maturity stage, to evaluate the difficulty of selenium enrichment in brown rice and other organs of each treatment. The results showed that the selenium content in each organ of brown rice increased with the increase of the concentration of selenium nutrient solution sprayed. Among them, the grain hull and leaves had the greatest response to selenium fertilizer, and the foliar selenium fertilizer increased the enrichment coefficient of selenium in brown rice and the transfer coefficient of selenium in each organ; Leaf selenium fertilizer had different effects on different mineral elements in brown rice; High concentration of selenium could effectively reduce the content of mercury, lead, cadmium and chromium in plant leaves, while low concentration of selenium could reduce the content of arsenic. [ABSTRACT FROM AUTHOR]

Meperfluthrin is one of the top-selling sanitary insecticide species in the domestic market, drawing widespread attention due to its environmental safety risk. In this paper, zebrafish was used as an experimental model to assess the cardiac developmental toxicity and oxidative damage caused by acute exposure to meperfluthrin in embryos and larvae. The acute toxicity assessment revealed that meperfluthrin is highly toxic to zebrafish embryos with a 72 h-LC50 of 0.756 μg/mL. After exposure to different doses of meperfluthrin (0, 0.2, 0.4, 0.8 μg/mL) for 72 h, the larvae from 0.4 and 0.8 μg/mL dose-treated groups showed deformities, including spine and tail curvature, pericardium and yolk sac edema, significant reduction of heart rate and hatchability, and incomplete embryonic development (shorter body length, smaller head, and eye area). Moreover, meperfluthrin of 0.2, 0.4, and 0.8 μg/mL induced atrial and ventricular differentiation in larvae, and down-regulated the expression of cardiac function-related genes myl7, myh6, and vmhc. It also increased the content of malondialdehyde (MDA), as well as decreased the activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), resulting in the accumulation of reactive oxygen species (ROS) in the heart of larvae. The results of this paper indicate that meperfluthrin exposure has significant toxicity to the cardiac development of zebrafish embryos and is associated with oxidative damage. [ABSTRACT FROM AUTHOR]

目的　观察腹腔镜肿瘤根治术麻醉诱导、维持期间静脉泵注艾司氯胺酮的老年直肠癌患者肺功能指标 (肺驱动压、气道平台压、气道峰压、肺顺应性、肺氧合指数、肺部超声评分) ，以明确艾司氯胺酮对肺功能的改善作 用。方法　择期行腹腔镜肿瘤根治术的老年直肠癌患者68例，采用随机数字表法分为艾司氯胺酮组 (A组) 和对照 组 (C组) 。麻醉诱导时，A组经外周静脉给予艾司氯胺酮0. 2 mg/kg， 麻醉维持阶段以0. 2 mg (/ kg. h) 的速率静脉泵 注艾司氯胺酮至手术结束前30 min，C组给予等容量生理盐水，应用时间、用法同A组。比较两组气管插管后5 min (气腹前) (T1) 、气腹及摆体位后5 min (T2) 、气腹60 min (T3) 、气腹结束后5 min (T4) 的肺驱动压、气道平台压、气道 峰压、肺顺应性；采集桡动脉血行血气分析，比较两组麻醉诱导前 (T0) 、T2、T3、T4的氧合指数。记录两组患者T0、手 术结束时 (T5) 、拔管后30 min (T6) 的肺部超声评分。采集两组T0、T5、术后24 h (T7) 的外周静脉血，采用ELISA法检测 血清IL-6、TNF-α。结果　与C组比较，T3、T4时A组的肺驱动压、气道平台压、气道峰压均降低，肺顺应性、氧合指 数升高；T5、T6时的肺部超声评分降低，T5、T7时IL-6、TNF-α水平降低 (P 均<0. 05) 。结论　腹腔镜肿瘤根治术麻醉 诱导、维持期间静脉泵注艾司氯胺酮的老年直肠癌患者肺功能明显改善，肺驱动压降低、肺顺应性提高、肺通气状态 改善、炎症减轻。 [ABSTRACT FROM AUTHOR]

Phase change asphalt mixture is a new functional pavement material that can regulate the temperature of asphalt concrete pavement. Two composite-shaped phase change materials( CPCM) of paraffin / expanded graphite / high-density polyethylene ( PHDP) and DTC road temperature regulating material were selected. Firstly, Fourier transform infrared spectroscopy ( FTIR) was used for testing, and qualitative analysis was conducted on the reaction between CPCMs and asphalt. Secondly, phase change asphalt mixtures with grading types AC-16 and AC-20 were prepared, and the impact of different aggregate grading on the road performance and temperature control effect of phase change asphalt mixtures were evaluated through road performance tests and temperature control tests. The results indicate that there is a physical reaction between both types of CPCM and asphalt. Compared to the grading type AC-16, AC-20 can significantly improve the road performance and temperature control effect of phase change asphalt mixtures containing PHDP, but the improvement effect on phase change asphalt mixtures containing DTC is not significant. [ABSTRACT FROM AUTHOR]

随着精准、微创医疗理念的普及,脊柱微创手术近年发展迅速,已成为脊柱手术的重要组成部分。精准的椎弓根置钉是脊柱手术成败的关键因素之一。而徒手置钉与穿刺的准确率较低,风险较高,射线暴露时间长,反复穿刺易造成椎体正常骨质的二次破坏。骨科手术机器人的应用,可减少置钉偏差,提高置钉精确度,降低术中神经损伤风险,减少术中透视次数,具备精准、稳定、耐疲劳、安全及损伤小等优势,成为微创脊柱手术的一个重要发展方向。 [ABSTRACT FROM AUTHOR]

Objective: To explore the clinical efficacy and feasibility of microvascular decompression (MVD) combined with neurophysiological monitoring in the treatment of hemifacial spasm(HFS), and provide reference for clinical treatment. Methods: 80 cases of patients with HFS treated in Guangdong No.2 People's Hospital from June 2014 to September 2016 were selected, and divided into observation group and control group according to the random number table method, 40 cases in each group. The two groups were given MVD treatment, and the observation group was given neurophysiological monitoring combined with MVD. In which operation stage the lateral spread response (LSR) of observation group disappeared were recorded, and the situation of Brainstem Auditory Evoked Potential (BAEP) waveforms in the V wave decreased by more than 50% were also recorded. The clinical effects of the two groups 1 d and 3 months after operation and the complication rate 1d after operation were compared. Results: 6 cases (15.00%) of LSR waveform disappeared during the exploration of the cerebellopontine angle, and 33 cases (82.5%) disappeared during the nerve decompression, 1 case (2.50%) disappeared at the end of surgery. There were 5 cases BAEP waveforms in the V wave decreased more than 50%. The total effective rate 1d after operation of the observation group was significantly higher than that of the control group, the difference was statistically significant (P<0.05). There was no significant difference in the total effective rates 3 months after operation between the two groups. The total effective rates of the two groups 3 months after operation were significantly higher than 1d after operation, he difference was statistically significant (P<0.05). There was no significant difference in the incidence of facial paralysis and tinnitus between the two groups 1 d after operation (P>0.05). The incidence of vertigo and hearing loss in the observation group was lower than that in the control group, and the difference was statistically significant (P<0.05). Conclusion: The clinical curative effect MVD treatment of patients with HFS under neurophysiological monitoring is obvious, and the complication rate is low, while the safety is high. Compared with MVD alone, it has obvious advantages, which is worthy of clinical promotion. [ABSTRACT FROM AUTHOR]

Objective To investigate the cytomorphological and immunocytochemical features of tumor cells in the ascites of ovarian plasmacytoma (SOC). Methods Specimens of serous cavity effusions were collected from 61 tumor patients admitted to the Affiliated Wuxi People's Hospital of Nanjing Medical University from January 2015 to July 2021, including ascites from 32 SOC, 10 gastrointestinal adenocarcinomas, 5 pancreatic ductal adenocarcinomas, 6 lung adenocarcinomas, 4 benign mesothelial hyperplasia and 1 malignant mesothelioma patients, pleural effusions from 2 malignant mesothelioma patients and pericardial effusion from 1 malignant mesothelioma. Serous cavity effusion samples of all patients were collected, conventional smears were made through centrifugation, and cell paraffin blocks were made through centrifugation of remaining effusion samples. Conventional HE staining and immunocytochemical staining were applied to observe and summarize cytomorphological characteristics and immunocytochemical characteristics. The levels of serum tumor markers carbohydrate antigen 125 (CA125), carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA19-9) were detected. Results Of the 32 SOC patients, 5 had low-grade serous ovarian carcinoma (LGSOC) and 27 had high-grade serous ovarian carcinoma (HGSOC). 29 (90.6%) SOC patients had elevated serum CA125, but the difference was not statistically significant between them and patients with non-ovarian primary lesions included in the study (P>0.05); The serum CEA was positive in 9 patients with gastrointestinal adenocarcinoma and 5 patients with lung adenocarcinoma, and the positive rate was higher than that in SOC patients (P<0.001); The serum CA19-9 was positive in 5 patients with gastrointestinal adenocarcinoma and 5 patients with pancreatic ductal adenocarcinoma, and the positive rate was higher than that in SOC patients (P<0.05). The serum CA125, CEA and CA19-9 were within the normal range in 4 patients with benign mesothelial hyperplasia. LGSOC tumor cells were less heterogeneous and aggregated into small clusters or papillary pattern, and psammoma bodies could be observed in some LGSOC cases. The background cells were fewer and lymphocytes were predominant; the papillary structure was more obvious after making cell wax blocks. HGSOC tumor cells were highly heterogeneous, with significantly enlarged nuclei and varying sizes, which could be more than 3-fold different, and nucleoli and nuclear schizophrenia could be observed in some cases; tumor cells were mostly clustered into nested clusters, papillae and prune shapes; there were more background cells, mainly histiocytes. Immunocytochemical staining showed that AE1/AE3, CK7, PAX-8, CA125, and WTI were diffusely positively expressed in 32 SOC cases. P53 was focally positive in all 5 LGSOCs, diffusely positive in 23 HGSOCs, and negative in the other 4 HGSOCs. Most of adenocarcinomas of the gastrointestinal tract and lung had a history of surgery, and tumor cells of pancreatic ductal adenocarcinoma tend to form small cell nests. Immunocytochemistry can assist in the differential diagnosis of mesothelial-derived lesions with characteristic "open window" phenomenon. Conclusion Combining the clinical manifestations of the patient, the morphological characteristics of the cells in the smear and cell block of the ascites can provide important clues for the diagnosis of SOC, and the immunocytochemical tests can further improve the accuracy of the diagnosis. [ABSTRACT FROM AUTHOR]

Traditional correlation power analysis( CPA) had the problems of long analysis time and complexity computation.Combining the CPA,this paper proposed a method of correlation analysis based on difference variability,which used the difference between encryption key and hypothetical key. This method used the reciprocal relation between different bits and correlation relation. On the basis of given hypothesis key,it changed the value of every bit to obtain the correct key,which only needed 9 calculations to capture the 8 bit sub-key. Advanced encryption standard( AES) is cracked respectively in traditional CPA experiment and improved experiment. The result shows that the new method improves the efficiency on the basis of cracking the key successfully. [ABSTRACT FROM AUTHOR]

This paper used dynamic time warping( DTW) algorithm to align side channel signals in differential power analysis( DPA) attacks. Based on the principle of DTW algorithm which produced a warp path for recomposing signal traces pair,it proposed a flexible alignment method. The practical experiments based on simulated misalignment signals obtained from a microcontroller( AT89C52) which implemented the data encryption standard( DES) cipher in software and inserted the random time delays. As the results show,running DPA on misaligned traces requires a much larger trace set when misalignment is appreciably increased,in contrast,performing DPA on the traces modified by flexible alignment can make it very low-cost in terms of the number of traces that need to be processed. The result also confirms the effectiveness of method mentioned above. [ABSTRACT FROM AUTHOR]

In view of the data correlation of cryptographic chip5 s optical radiation,using time-correlated single photon counting techniques to build photonic emission analysis attacks experimental platform, this paper analyzed template based photonic emission attacks. It discussed the trace of photonic emission to portray template,built strategies and matching methods. Then,it portrayed template by using Hamming weight model and chose the S-box of the first round of advanced encryption standard (AES) encryption algorithm as the point of attack to implement successfully the template based photonic emission attacks. The result shows the template based photonic emission attacks is more efficient than other models. [ABSTRACT FROM AUTHOR]

Objective: To explore the application effect of applying case based learning (CBL) in the teaching of cell research progress. Methods: Selected 80 students who took "cell research progress" as an elective course in our school from 2013 to 2015, they were divided into the experimental group and the conventional group, each of the 40 cases,according to the different elective course time. Lecture-based learning (LBL) was implemented in the conventional group, the CBL teaching mode was implemented in the experimental group, teaching effects and test scores were compared between the two groups. Results: The ability of analyze problems,establishment of clinical thinking, classroom enthusiasm and teaching satisfaction scores in the experimental group were higher than the conventional group, the differences were statistically significant(P<0.05). The clinical case analysis and clinical operation skill scores in the experimental group were higher than conventional group, the differences were statistically significant (P<0.05). Conclusion: Using CBL teaching mode in the students who take "cell research progress" as an elective course,is able to establish the clinical thinking,improve the ability of clinical operation, as well as the satisfaction to the teaching of students. [ABSTRACT FROM AUTHOR]

Objective: To explore the application effect of applying case based learning (CBL) in the teaching of cell research progress. Methods: Selected 80 students who took "cell research progress" as an elective course in our school from 2013 to 2015, they were divided into the experimental group and the conventional group, each of the 40 cases,according to the different elective course time. Lecture-based learning (LBL) was implemented in the conventional group, the CBL teaching mode was implemented in the experimental group, teaching effects and test scores were compared between the two groups. Results: The ability of analyze problems, establishment of clinical thinking, classroom enthusiasm and teaching satisfaction scores in the experimental group were higher than the conventional group, the differences were statistically significant(P<0.05). The clinical case analysis and clinical operation skill scores in the experimental group were higher than conventional group, the differences were statistically significant (P<0.05). Conclusion: Using CBL teaching mode in the students who take "cell research progress" as an elective course,is able to establish the clinical thinking,improve the ability of clinical operation, as well as the satisfaction to the teaching of students. [ABSTRACT FROM AUTHOR]

间质性肺疾病(ILD)是一组异源性疾病,原因各异,机制不同,病变多样,不同病因或疾病可产生相同或相似的临床病理表现,因而诊断缺乏特异性;而且,不同疾病或者病理类型的预后和治疗反应差异很大.肿瘤标志物是存在于血液和组织中可以反映肿瘤存在的化学类物质,通过测定其存在和含量,对肿瘤的临床治疗和预后判断有重要作用.然而,ILD患者多出现血清肿瘤标志物的升高,有助于ILD的诊断、严重程度评估、预后判断,也可用于ILD合并肺癌的早期预警. [ABSTRACT FROM AUTHOR]

In the bridge technical evaluation work, there is a lack of matching degree between the bridge parts and component weights and the actual situation. Analytic hierarchy process (AHP), expert group analysis and entropy weight method were used to build a comprehensive analysis system of bridge parts and component weights. The “subjective weight” of bridge parts and components was obtained through AHP and expert group analysis. The “objective weight” of bridge parts and components was obtained by using entropy weight method to analyze the bridge structure health monitoring data. The bridge parts and components weight system that considers both subjective and objective factors is obtained through weight fusion. Taking the Qingzhou Channel Bridge of Hong Kong Zhuhai Macao Bridge as the analysis object, the weight system was given by comparing the current bridge technical status evaluation standards. The results show that the weight value obtained by the group analysis method is basically consistent with the standard weight value. The weight value of superstructure obtained by entropy weight method is slightly larger than the standard weight value, and the weight value of substructure and bridge deck system is basically the same. The weight value of the superstructure obtained by the weight fusion method is too large, the weight value of the bridge deck system is too small, and the weight value of the substructure is basically the same. The analysis results obtained by weight fusion comprehensively consider the subjective and objective factors, which are closer to the actual situation of the project and reasonably reflect the weight of bridge parts and components. [ABSTRACT FROM AUTHOR]

Objective：To investigate the improvement effect of tetramethylpyrazine（TMP)on oxidative damage of kidney tissue in the nephrolithiasis model rats, and to clarify its possible mechanism. Methods：A total of 40 healthy male SD rats were randomly divided into control group, model group, TMP group and positive control group. After pre-experiment，except control groups，the rats in other groups were intraperitoneally injected with 80 mg·kg－1 glyoxylate stock solution to establish the rat models of kidney stones； the rats in TMP group were intraperitoneally injected with TMP hydrochloride injection 100 mg·kg－1，the rats in positive control group were intragastrically perfused with 3. 12 g·kg－1 of Shenshitong granules, and the rats in control group were intraperitoneally injected with 2. 5 mL·kg－1 of 0. 9% sodium chloride injection，lasted for 10 d. The rat kidney tissue sections were taken, Von Kossa staining and HE staining were performed to observe the calcium deposition and the pathomorphology of kidney tissue, and the levels of malondialdehyde（MDA)and the activities of superoxide dismutase （SOD)and glutathione peroxidase （GSH-Px)in kidney tissue of the rats in various groups were detected. Western blotting method was used to detect the expression levels of nuclear protein nuclear factor E2-related factor 2 （Nrf2), holoprotein heme oxygenase-1 （HO-1)and NAD（P)H quinone oxidoreductase 1 （NQO1)proteins in kidney tissue of the rats in various groups. Results：Compared with control group，obvious calcium deposition was seen in the Von Kossa staining of kidney tissue of the rats in model group，and the quantitative grading score of crystal was significantly increased （P<0. 01)；the HE staining results showed obvious pathological damage in kidney tissue cells，the level of MDA in kidney tissue was significantly increased （P<0. 01), the activities of SOD and GSH-Px were significantly decreased （P<0. 01)，and the expression levels of Nrf2, HO-1 and NQO1 proteins in kidney tissue were significantly decreased （P< 0. 01). Compared with model group, calcium deposition was seen in the Von Kossa staining of kidney tissue of the rats in TMP group and positive control group, and the quantitative grading scores of crystal were decreased （P<0. 05)；the HE staining results showed that the pathological damage of kidney tissue cells was alleviated, the levels of MDA were significantly decreased （P<0. 05)，the activities of SOD and GSH-Px were significantly increased （P<0. 05), and the expression levels of Nrf2, HO-1 and NQO1 proteins in kidney tissue were significantly increased （P<0. 05). Conclusion：Kidney stones can cause oxidative stress injury in the rat kidney tissue, resulting in the changes in oxidative stress indicators and Nrf2/antioxidant response element（ARE)signaling pathway protein expression levels in kidney tissue. TMP can activate this signaling pathway after intervention, so as to improve the oxidative stress injury of kidney tissue. [ABSTRACT FROM AUTHOR]