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Objective: To explore the correlation between CD3+ and CD8+ regulatory T cells with postoperative recurrence in colon cancer patients and construct the nomogram model to analyze predictive value. Methods: A total of 170 patients with colon cancer in our hospital from December 2018 to November 2020 were selected, and divided into recurrence group (n=22) and no recurrence group (n=148) according to the recurrence situation in 3 years after surgery. Multivariate Logistic regression was used to analyze the influencing factors, a nomogram prediction model was constructed and receiver operating characteristic (ROC) curve was used to evaluate the prediction efficiency. Results: The proportion of CD3+ in the relapse group was lower than that in the no relapse group, and the proportion of CD8+ was higher than that in no relapse group (P<0.05). TNM staging, tumor focus location, lymph node metastasis, preoperative carcinoembryonic antigen (CEA) and CD8+ were independent risk factors for recurrence of colon cancer, and CD3+ was protective factor (P<0.05). The area under the curve (AUC) predicted by the nomogram model for postoperative recurrence risk of colon cancer patients was 0.892 (95% CI: 0.808~0.975). Conclusions: CD3+, CD8+ regulatory T cells level in patients with recurrent colon cancer after surgery is abnormal. TNM staging, tumor focus location, lymph node metastasis, preoperative CEA, CD8+, and CD3+ were the influencing factors for recurrence in patients with colon cancer. The nomogram prediction model constructed according to the influencing factors has high predictive value for the prognostic recurrence of colon cancer patients, and helps clinicians to better evaluate the risk of postoperative recurrence and develop personalized treatment plan. [ABSTRACT FROM AUTHOR]

Objective To analyze the epidemiological characteristics of perinatal birth defects in Henan Province from 2011 to 2020 and to provide basis for the prevention of birth defects. Methods Perinatal birth defect surveillance data from 37 national monitoring sites in Henan Province were collected for newborns delivered from 2011 to 2020. Joinpoint regression was used to describe the trends in birth defect incidence, and Chi-square tests compared incidence rates among different groups (including region, infant sex, and maternal age). Results A total of 29 418 cases of birth defects were monitored in Henan Province from 2011 to 2020, resulting in a total incidence rate of 216.81 per 10 000 births. The annual incidence showed a significant upward trend (AAPC=20.07%, P < 0.001). Incidence rates were significantly higher in urban areas compared to rural areas, in boys compared to girls, and in mothers aged ≥ 35 years compared to those < 35 years (χ²=5 545.926, 257.052, and 403.326 respectively, all P < 0.001). The top 10 birth defects were congenital heart disease (118.47 per 10 000), other malformations (47.62 per 10 000), polydactyly (14.34 per 10 000), cleft lip and palate (10.23 per 10 000), other malformations of the external ear (9.74 per 10 000), syndactyly (5.73 per 10 000), congenital hydrocephalus (5.32 per 10 000), hypospadias (5.24 per 10 000), equinovarus (4.35 per 10 000), and limb shortening (2.69 per 10 000). Conclusion The incidence of birth defects in Henan Province demonstrated a significant upward trend from 2011 to 2020. Comprehensive measures for birth defect prevention and treatment are essential to reduce incidence rates and enhance the quality of the population. [ABSTRACT FROM AUTHOR]

Based on whether the expected output includes agricultural ecosystem services, this paper makes a comparative analysis of agricultural ecological efficiency in Guizhou karst mountain area, in order to provide theoretical reference and data support for promoting the green development of agriculture in this area. The super-efficiency SBM model and GML index were used to measure the two agricultural eco-efficiency of nine cities and prefectures in Guizhou from 2000 to 2019,and the Tobit regression model was used to explore its influencing factors. The results show that: The traditional agro-ecological efficiency is only effective in Guiyang. After considering agro-ecosystem services, the average agricultural efficiency of other cities and states enters the effective zone except Liupanshui, Anshun, Qianxi and Qiannan. During the study period, the traditional agricultural total factor productivity (GML) of Guizhou increased by 2% annually, especially the exponential growth of GML after 2012. In addition to Zunyi, Qiandongnan and Tongren,the traditional agricultural total factor productivity (GML) of the other cities and states was greater than 1,and the improvement of agricultural ecological efficiency in each city and state was mainly driven by technological progress. In addition to Zunyi and Tongren, the improved GML index of other cities and states is lower than the traditional one. Industrial structure, financial support for agriculture, and agricultural mechanization density were positively correlated with both traditional and improved agroecological efficiency; farmers’ income level and fertilizer use intensity were negatively correlated with both traditional and improved agroecological efficiency; per capita cultivated land level and vegetation coverage were positively correlated with improved agroecological efficiency, but negatively correlated with traditional agroecological efficiency. [ABSTRACT FROM AUTHOR]

Lactic acid bacteria (LAB)plays an important role in the intestinal health of animals. In order to screen out excellently properties LAB strains, in this study, LAB from the feces of healthy south China tigers (Panthera tigris amoyensis) were isolated, cultured, purified and identified. The biological characteristics of these strains were evaluated by studying its bacteriostatic properties against Escherichia coli, Staphylococcus aureus, Salmonella sp. and Edwardsiella tarda, acid resistance and bile salts resistance, hemolytic activity and drug against resistance test of 24 kinds of drugs. The results showed that a total of 78 strains of LAB were isolated, among which 15 strains had good antibacterial performance and the antibacterial circles were greater than 15 mm against the four pathogenic bacteria mentioned above, including six strains(22YY08，22DC20，22XX19，22Hui09，22LL14 and 22XX03) of Lactobacillus reuteri, three strains(22HH02，22YY13 and 22YY09)of L. crispatus, two strains(22Hui15 and 22LL07) of Ligilactobacillus animalis, one Pediococcus pentosaceus(22DC18), one Lactobacillus mucosae (22DC10), one L. johnsonii(22DC09), and one Lactococcus garvieae (GaL110). Further, eight LAB strains were screened out and showed good tolerance at pH 2. 5 and bile salt concentration of 0. 1%, and had no hemolytic activity. Among them, three strains of Lactobacillus reuteri(22Hui09，22YY08 and 22LL14) were resistant to multiple drugs while others were susceptible. In conclusion, three strains of L. reuteri(22DC20，22XX03 and 22XX19)，Pediococcus pentosaceus 22DC18, and Lactococcus garvieae GaL110 obtained in this study are promising candidates for the development of probiotic preparations in south China tiger. [ABSTRACT FROM AUTHOR]

BACKGROUND: At present, there are few reports on the application of compressed sensing technology in ankle MRI. OBJECTIVE: To investigate the effect of different acceleration factors in compressed sensing (CS) on image quality and scanning time of conventional 2D-MRI of the ankle joint. METHODS: Totally 24 healthy volunteers (38 ankles) underwent conventional 2D-TSE sequence scanning at 3.0T MR. Based on SENSE parallel imaging (S group) and compressed sensing technique (CS group), sequence images of T1WI-TRA (S1.3, CS1.3, CS1.9, CS2.7), PDWI-Dixon-SAG (S1.8, CS1.8, CS2.6, CS3.2) and PDWI-SPAIR-COR (S1.3, CS1.3, CS1.6, CS2.0) were obtained separately. Other scan parameters of each sequence remained consistent. The tendon, cartilage, ligament and muscle structures of the ankle joint images were subjectively scored by 5 points. The standard deviation of background noise and signal intensity of bone, cartilage, ligament, tendon, muscle, effusion and fat structures were measured, and the signal-to-noise ratio and contrast-to-noise ratio were calculated. The subjective score and objective evaluation of different acceleration factor images were statistically analyzed. SENSE group was used as the standard reference for the image quality of each sequence. RESULTS AND CONCLUSION: (1) When acceleration factor was the same, there were no statistically significant differences in subjective score, signal-to-noise ratio and contrast-to-noise ratio between the S group and the CS group (P > 0.05). (2) The acceleration factor of CS (TRA), CS (SAG) and CS (COR) sequences at CS1.9, CS2.6 and CS1.6 showed no significant difference in the image quality of cartilage, tendon, ligament and other main observed structures of the ankle joint (P > 0.05), and the scanning time was 1 minute and 32 seconds, 1 minute and 42 seconds, 1 minute and 48 seconds, respectively. When the acceleration factor of CS (TRA), CS (SAG) and CS (COR) sequences increased to CS2.7, CS3.2 and CS2.0, respectively, the subjective scores of all anatomical structures were still greater than 3, but signal-to-noise ratio and contrast-to-noise ratio of each sequence decreased significantly (P < 0.05). (3) These findings confirm that when acceleration factor is the same, compared with CS group, the image quality obtained by CS group is generally higher than that of S group. When acceleration factor increases, the scanning time of CS sequence decreases gradually, and the image quality also decreases. On a 3.0T MR, CS technology recommended CS1.9, CS2.6, and CS1.6 accelerators for conventional 2D sequences T1WI-TRA, PDWI-Dixon-SAG, and PDWI-SPAIR-COR, respectively, reducing the overall time by about 27% (1 minute and 53 seconds). [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of lipopolysaccharide (LPS) of Porphyromonas gingivalis(P. g)on the expression levels of ferroptosis-related factors in the macrophages, and to clarify the influence of ferroptosis-related factors in the pathogenesis of periodontitis. Methods: The mouse mononuclear/macrophage line RAW264. 7 cells were cultured and divided into experimental group and control group. The cells in experimental group were treated with 10 mg·L -1 P. g-LPS for 6, 12, and 24 h, respectively；the cells in control group were not treated. Real-time fluorescence quantitative PCR (RT-qPCR) method was performed to detect the expression levels of long chain acyl-CoA synthetase 4 (ACSL4), glutathione peroxidase 4 (GPX4), and transferrin receptor 1 (TfR1) mRNA in the RAW264. 7 cells in two groups. The levels of reactive oxygen species(ROS)in the RAW264. 7 cells in two groups were detected by 2', 7'-dichlorodihydrofluorescein diacetate(DCFH-DA) fluorescence probe method. The levels of malondialdehyde(MDA)in the RAW264. 7 cells in two groups were detected by thiobarbituric acid (TBA) method and the levels of ferrous ion (Fe2+) in the RAW264. 7 cells in two groups were detected by Ferrousion fluorescent probe (FeRhonox-1) method. The expression levels of ACSL4, GPX4, and TfR1 proteins in the RAW264. 7 cells in two groups were detected by Western blotting method. Results: Compared with control group, the expression levels of GPX4 mRNA in the RAW264. 7 cells in experimental group after treated for 6, 12, and 24 h were decreased(P<0. 05), the expression levels of ACSL4 and TfR1 mRNA in the RAW264. 7 cells in experimental group after treated for 12 and 24 h were increased (P<0. 05)；the expression levels of ACSL4 and TfR1 proteins in the RAW264. 7 cells in experimental group after treated for 12 and 24 h were increased (P<0. 05)；the expression levels of GPX4 protein in the RAW264. 7 cells in experimental group after treated for 12 and 24 h were decreased(P<0. 05). Compared with control group, the levels of ROS, MDA, and Fe2+ in the RAW264. 7 cells in experimental group were significantly increased(P<0. 05), and showed an increasing trend with time prolongation. Conclusion: The expression levels of ferroptosis-related factors ACSL4 and TfR1 are increased and the expression level of GPX4 is decreased in a time-dependent manner induced by P. g-LPS, suggesting that the changes of ferroptosis-related factors may be related to the pathogenesis of periodontitis. [ABSTRACT FROM AUTHOR]

[Objective] The seeds and seedlings of Rhus typhina were used as experimental materials, and the effects of heavy metals copper(Cu2+) and zinc(Zn2+) on the growth and physiological indexes of Rhus typhina seedlings were studied.[Method] Hydroponics was used in this study, the R.typhina seed was treated with different concentrations of Cu2+, Zn2+ single and compound treatment. The germination rate, germination potential and germination index of seeds were measured after 12 days, and the radicle length, bud length, fresh weight, superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and malondialdehyde contents of seedlings were measured.[Result] Under the treatment of single Cu2+and Zn2+ solution concentration, the germination rate, germination potential, germination index, radicle length, bud length and fresh weight of R.typhina showed a trend of ‘low promotion and high inhibition’, that was, low concentrations of Cu2+ (<0.25 mmol/L) and Zn2+ (<1 mmol/L) promoted the growth of R.typhina, while high concentrations of Cu2+ (>0.5 mmol/L) and Zn2+ (>2 mmol/L) inhibited the growth of R.typhina; The growth indexes under compound stress(Cu2++Zn2+) were lower than those in the control group, which significantly inhibited the growth of R.typhina. With the increase of Cu2+ and Zn2+ solution concentration, the activities of superoxide dismutase (SOD) and catalase (CAT) increased first and then decreased, and under the combined stress treatment at the same level of Cu2+ concentrations, decreased with increasing concentration; The peroxidase (POD) activity and malondialdehyde content were positively correlated with the mass concentration of the treatment solution, under compound stress, it showed an upward level and reached the maximum under Cu2+ (2 mmol/L), Zn2+ (4 mmol/L) and compound stress(Cu2+ 2 mmol/L+ Zn2+ 4 mmol/L).[Conclusion] R.typhina had high tolerance to single stress and compound stress of Cu2+ and Zn2+, and could be considered as an alternative plant for remediation of soil heavy metal pollution. [ABSTRACT FROM AUTHOR]

As the core city of the central plains urban agglomeration, the change of water resources in Zhengzhou is closely related to the development of urban economy and society. In order to scientifically analyze the current situation of water resources carrying capacity in Zhengzhou, based on the analysis of the current situation of water resources in Zhengzhou, this paper constructed a quantity-quality-domain-flow water resources carrying capacity index system, and used the subjective and objective weighting method and TOPSIS model to evaluate the water resources carrying capacity of Zhengzhou from 2010 to 2019. The results show that the water resources carrying capacity of Zhengzhou has fluctuated and increased in research period. After 2015, the water resources carrying capacity has continued to increase, and the water resources carrying capacity reached to grade I in 2018. Through further analysis of water resources carrying capacity in all dimensions, it is found that Zhengzhou belongs to the resources-type water shortage. Next, it should further improve the urban water-saving level and optimize the allocation of water resources. [ABSTRACT FROM AUTHOR]

Objective To observe whether region cerebral blood flow （rCBF） is altered in brain before surgery in postoperative cognitive dysfunction（POCD）patients，and whether there is a correlation with the score of neuropsychiatric scale in elderly patients. Methods A total of 83 elderly（≥65 years） patients scheduled for spinal stenosis surgery during 2017-2020 were enrolled in the study. All the participants accomplished a battery of neuropsychological tests by well-trained neuropsychologist 1 day before and 1 week after surgery. Pulsed arterial spin-labeling（ASL） MRI was scanned at least one day before surgery. POCD was defined as≥2 neuropsychological test Z-scores>1.96. The patients were devided into POCD and NPOCD group. The ASLtbx toolkit and SPM8 were applied to preprocess and correct the images，which were then standardized to the MNI brain template space to obtain standardized cerebral perfusion images.ALS in the two groups were compared. Results POCD was identified in 11 of 50 patients（22%）. The CBF of the right middle cingulate gyrus in POCD group was lower than that in NPOCD group（P<0.001）. The correlation coefficient r of CBF with Z score of JLOT，BVMT-D and DST-B was -0.631，-0.480 and -0.533 （P<0.05），respectively，which was statistically significant. Conclusion Patients' preoperative MRI cingulate gyrus local CBF may contribute to the prediction of early POCD in elderly patients，and there is a good correlation between cingulate gyrus local CBF and neuropsychiatric scales. [ABSTRACT FROM AUTHOR]

BACKGROUND: As an extension of the "materialization" of image technology, three-dimensional (3D) printing is inseparable between the two. To meet the needs of clinical application, the combination of different principles of imaging technology and 3D printing technology makes it more possible in medical application. OBJECTIVE: To summarize the progress of medical application research of the combination of different imaging principles and 3D printing, and analyze its advantages and disadvantages. METHODS: The relevant articles from the databases of Web of Science, PubMed, CNKI, SinoMed and Wanfang were searched by computer. The subject headings "3D printed, Three-dimensional printing, Additional Manufacturing, Ultrasonography, Ultrasound, MRI, Magnetic Resonance Imaging, CT, Computed Tomography, PET/CT, SPECT/CT, Radiotherapy, Digital Subtraction, DSA" in English and "3D printing, ultrasound, MRI, radiotherapy, nuclear medicine" in Chinese were retrieved in the past five years, and finally 61 articles were included for review. RESULTS AND CONCLUSION: (1) This paper summarizes the medical application of 3D printing based on different types of image data, which is mainly divided into clinical application, equipment application and teaching application. The advantages and disadvantages of different image data sources are discussed and the difficulties in the combination of the two are summarized. (2) At present, the clinical application of medical 3D printing based on radiological imaging is wide, and the use is relatively mature. (3) With the in-depth application of 3D printing technology, non-ionized imaging methods such as MRI and ultrasound also show great potential in the application. [ABSTRACT FROM AUTHOR]

[Objective]Taking tobacco as experiment material, extraction process of tobacco polypeptide and its antioxidant activity was studied in this research. [Method]Tobacco polypeptide was extracted by enzyme-assisted water extraction and alcohol precipitation. Single factor test was used to investigate the effects of enzyme addition, enzymolysis pH, enzymolysis time, extraction temperature and substrate concentration on the extraction rate of tobacco polypeptide. The optimal extraction process of tobacco polypeptide was determined by response surface method. The scavenging activity of tobacco polypeptide on DPPH and hydroxyl radicals was detected. [Result]The optimal extraction process for tobacco polypeptide was as follows (based on 1 g tobacco sample):enzyme addition 4 200 U, enzymolysis pH 8. 2, enzymolysis time 2. 5 h, extraction temperature 49 ℃ and the substrate concentration 4. 2%. Under the optimized extraction process, the extraction rate of tobacco polypeptide was 88. 23 mg / g. The scavenging rates of DPPH and hydroxyl radicals of the tobacco polypeptide were 83. 09% and 73. 84%, respectively. Tobacco polypeptide was of a certain antioxidant capacity. [Conclusion] This study provides a certain theoretical basis and technical guidance for the medicinal value of tobacco leaves and the drug development of tobacco peptides. [ABSTRACT FROM AUTHOR]

Water resources carrying capacity in irrigation area is affected by various factors such as temperature, rainfall, evaporation, planting structure, economic and social development, etc. Starting from the concept of ecological irrigation area, on the basis of a comprehensive analysis of the "economic society-water resources-ecological environment" composite system of ecological irrigation area, a calculation model of water resources carrying capacity (PSO-COIM) based on ecology was established. Taking the Yellow River diversion irrigation area in Henan Province as an example, the water resources carrying capacity of the irrigation area was calculated by system analysis method and the water resources carrying capacity of the irrigation area was systematically analyzed. For the construction of ecological irrigation areas, besides improving the probability of water supply for diverting water from the Yellow River, it is also necessary to increase the utilization of storm flood resources and the reuse of reclaimed water in the irrigation areas so as to maximize the use of flood water resources and realize the sustainable development of water resources in the irrigation areas. [ABSTRACT FROM AUTHOR]

Twelve push-out specimens were tested and three-dimensional nonlinear finite element analysis was conducted to investigate the shear behavior of studs under polar temperature. The equipment included large-scale cold storage, insulation box and pressure testing machine. By changing the investigated parameters including temperatures and stud diameters, the failure modes, load-slip curves, ultimate shear capacity and slip were obtained. The results show that two kinds of failure modes including stud shear and mixed failure can be distinguished. In the temperature range from 20~ to -80°C, the shear capacity of studs increases and the ultimate slip decreases with the decrease of temperature. In the temperature range from -60°C to -80°C, the temperature has slighter influence on the shear behavior of studs. With the increase of the stud diameter, the bearing capacity and the ultimate slip increase under polar temperatures. By using ABAQUS software and adopting cryogenic constitutive relations, the finite element analysis results and regression curves agree well with the test results. [ABSTRACT FROM AUTHOR]

With the development, a relatively perfect policy system has been gradually established for mine ecological protection and restoration in China based on the two contexts of mine land reclamation and mine geological environment protection and restoration. strengthen source control and promote green mine construction; Second, comprehensively promote system protection and restoration; Third, accelerate ecological and environmental governance in key areas and regions; Fourth, improve the ecological compensation system and financial support policy Fifth, promote the establishment of market-based mechanisms and encourage and incentivize the participation of private capital. Based on the current policy orientation and problems existing in practice, this paper puts forward fund guarantee system for mine ecological restoration, improving the implementation rules of the market-oriented operation support policy for projects, promoting the standardization of mine ecological restoration standards and working procedures, and optimizing the whole life cycle management system for new and production mines. [ABSTRACT FROM AUTHOR]

Nowadays, there are many researches about diabetes and tumors, it includes the epidemiology of the correlation between the two diseases, the correlation between diabetes and different types of cancer, and the interaction of the treatment and so on.These researches revealed that there is a complicated relationship between them. From the existing studies, it can be found that there may be some mechanisms of action in the process of the occurrence and development of the diseases, thus affecting the incidence of cancer in patients with diabetes. But at the present stage, there are still a lot of controversy, so expliciting the exact correlation between these two diseases may have a certain role in the prevention and treatment of diabetes with cancer. In this paper, the current research progress on the relationship between tumor and diabetes mellitus was reviewed. [ABSTRACT FROM AUTHOR]

肝药酶CYP即细胞色素P450氧化酶(CYP450),属于单加氧酶(monooxygenase),也称肝微粒体混合功能氧化酶,多位于内质网和线粒体内膜上,参与药物、致癌物、类固醇激素和脂肪酸等多种内、外源性物质代谢。肝药酶CYP氧化还原酶(POR)是所有肝微粒体酶的唯一电子供体,通过还原型烟酰胺腺嘌呤二核苷酸磷酸(reduced nicotinamide adenine dinucleotide phosphate,NADPH)将电子传递给CYP 450酶,CYP 450酶得到电子后再与底物发生氧化还原反应,从而发挥代谢活性~[1]。POR基因具有遗传多态性,POR基因突变导 [ABSTRACT FROM AUTHOR]

目的：鉴定及优化表达小鼠来源的OX40融合蛋白。方法将已构建好的pET32a-OX40重组质粒DNA转入大肠杆菌BL21（DE3）感受态细胞，涂于含Amp平板筛选出阳性克隆，经Western blotting鉴定融合蛋白，对阳性克隆株通过改变异丙基硫代半乳糖苷（IPTG）浓度、培养温度及时间条件，观察目的融合蛋白表达的变化。结果转化的质粒经Western blotting鉴定，证实有目的蛋白表达。当温度、时间不变时，IPTG分别为0．1、0．2、0．4、0．6、0．8、1．0、2．0 mmol／L，7个不同浓度实验结果以0．4 mmol／L时蛋白表达量最高。当IPTG浓度、时间不变时，在20、25、30、35、40℃不同的温度点，以30℃时蛋白表达量最高。当温度、IPTG浓度不变时，1、2、3、4、5、6 h不同时间点，以4 h时蛋白表达量最高。结论小鼠来源的OX40融合蛋白在BL21细胞获得成功表达，并获得其最佳表达条件（IPTG浓度0．4 mmol／L、温度30℃、诱导4 h）。 [ABSTRACT FROM AUTHOR]

Objective To investigate the causal association of liver function and lipid metabolism levels with sleep disorders based on the Mendelian randomization analysis. Methods The analysis was conducted using the data from genome-wide association studies, with the exposure factors of liver function and lipid metabolism levels (alanine aminotransferase [ALT], aspartate aminotransferase [AST], gamma-glutamyl transpeptidase [GGT], albumin [Alb], serum total protein [TP], total bilirubin [TBil], alkaline phosphatase [ALP], triglyceride [TG], triglyceride-to-glycerol-3-phosphate [TG/G3P] ratio, total cholesterol [TC], high-density lipoprotein cholesterol [HDL-C], low-density lipoprotein cholesterol [LDL-C], poly-unsaturated fatty acids [PUFA], total fatty acids [TFA], PUFA/TFA ratio) and the outcome factor of sleep disorders (nonorganic) . The regression models including inverse variance weighted, MR-Egger, Simple mode, weighted median, and Weighted mode were used to perform the Mendelian randomization analysis. Results Serum Alb (odds ratio [OR] =0.728, 95% confidence interval [CI] : 0.535 — 0.989, P<0.05), HDL-C (OR=0.879, 95%CI: 0.784 — 0.986, P< 0.05), and PUFA/TFA ratio (OR=0.800, 95%CI: 0.642—0.998, P<0.05) were negatively associated with sleep disorders, while TG/G3P ratio (OR=1.222, 95%CI: 1.044—1.431, P<0.05) was positively associated with sleep disorders. The results of Mendelian randomization did not show a causal association of ALT, AST, GGT, TP, TBil, ALP, TG, TC, LDL-C, PUFA, and TFA with sleep disorders (all P>0.05) . The results of the MR-Egger intercept test showed no pleiotropy (P>0.05), and Mendelian randomization was a valid method for causal inference in this study. Conclusion According to the results of the Mendelian randomization analysis, liver function and lipid metabolism show significant association with sleep disorders. Liver function and lipid metabolism can be used as indicators for predicting the risk of sleep disorders and performing intervention. [ABSTRACT FROM AUTHOR]

While many current existing models focus on mining limited data in order to improve the click-rate prediction effect, the following problems arise simultaneously: the features are directly input into a unified neural network structure for mining and subsequently output regarding both the original and newly generated explicit ones, their representation will be great different. Additionally, if the multi-level neural network structure is adopted directly, it will also result in the loss of information at low layers such as the first layer and the second layer. The information with limited usefulness in some layers may produce noise to affect prediction if they are accumulated and combined directly. This paper designed a multi-level explicit interactive fusion recommendation model with a multi-level weight combination. It mined the original data and the generated multi-level explicit features into their corresponding self-attention layers, which had different layers of their corresponding structures. Meanwhile, it output after weighted each layer to complete the combination of layers, so as to improve the prediction effect of click rate . It shows that this model is able to mine explicit and implicit interactive features effectively and balance the ability of each order to represent features in an effective way, with the support of the comparison and analysis with several different models on two public data sets, as well as the ablation comparison experiment and super parameter comparison of the model. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To probe into the related risk factors of cardiotoxicity in patients with breast cancer after sequential trastuzumab therapy with anthracyclines. METHODS: Totally 169 patients with breast cancer undergoing sequential trastuzumab therapy with anthracyclines in this hospital from Apr. 2017 to Jan. 2020 were extracted to be divided into the cardiotoxicity group and the non-cardiotoxicity group according to the incidence of cardiotoxicity. The age, body mass index, tumor stage, history of hypertension, history of diabetes, history of hyperlipidemia, combined radiotherapy, categories of anthracyclines and other factors of two groups of patients were recorded and analyzed. Risk factors of cardiotoxicity in patients with breast cancer after sequential trastuzumab therapy with anthracyclines were analyzed by logistic regression. RESULTS: Among 169 patients with breast cancer after sequential trastuzumab therapy with anthracyclines, 57 patients developed cardiotoxicity, with the incidence of 33. 73%. There were statistically significant differences between the cardiotoxicity group and the non-cardiotoxicity group in the history of hyperlipidemia, combined radiotherapy and categories of anthracyclines ( P < 0. 05 ) . Further analysis showed that patients with hyperlipidemia had an increased risk of cardiotoxicity compared with those without hyperlipidemia (OR= 5. 068, 95% CI= 2. 209-11. 628). Patients treated with left thoracic radiotherapy had an increased risk of cardiotoxicity compared with those without combined radiotherapy ( OR= 3. 734, 95% CI= 1. 448-9. 628). Compared with patients received epirubicin, patients received pirarubicin had a lower risk of cardiotoxicity ( OR = 0. 407, 95% CJ= 0. 182-0. 911 ) . CONCLUSIONS: The incidence of cardiotoxicity in patients with breast cancer after sequential trastuzumab therapy with anthracyclines is higher. Treatment with epirubicin, history of hyperlipidemia and history of left thoracic radiotherapy are independent risk factors for cardiotoxicity in patients with breast cancer after sequential trastuzumab therapy with anthracyclines. [ABSTRACT FROM AUTHOR]

The “standard land” reform in Hangzhou is typical, and its experience has important implications for the whole country. This study analyzed the background and practice of the standard land reform in Hangzhou from the perspective of land supply-side reform. The results show that the standard land reform promotes the business environment, deepens the connotation of the “hero of land output” reform, and advances the high-quality development of the industry. Further policy suggestions are put forward, including perfecting the index system and dynamic adjustment mechanism of standard land, strengthening the digital empowerment, improving the space quality of standard land, and enhancing the reform supporting service and supervision system of standard land. [ABSTRACT FROM AUTHOR]

In the IoV system, the high speed movement of vehicle nodes and frequent changes in speed, direction and other states cause time-varying fading of the wireless channel in vehicular networks. To solve the problems of the currently used adaptive traffic beacons (ATB) prediction algorithm process with complex and many channel quality evaluation indicators, a new R-ATB prediction algorithm was proposed. The R-ATB algorithm was achieved by calculating the corresponding correlation time parameter of relevant time (RT) according to the Doppler shift degree of node motion. The package delivery rate (PDR) and the time delay parameters under the correlated channel prediction algorithm were analyzed and evaluated by setting the conditions and system modeling in OMNET++ universal network platform. The results show that compared with static beacons and traditional ATB algorithms, in the proposed R-ATB prediction algorithm, less amount of real-time evaluation metrics and smaller computation of the prediction process are used to reduce the communication channel congestion of IoV. The propagation timeliness of security beacons and the PDR of high-speed data transmission success rate are improved in typical high-density OBUs data interaction scenarios. [ABSTRACT FROM AUTHOR]

In order to deal with the imbalance of water resources in the water receiving area of the Yellow River Diversion Project in Henan Province and provide a reference for the rational allocation of water resources in the water received area, this paper used Lorentz Curve to calculate the Gini coefficient of 14 prefecture level cities in the water received area, and used the method of connection number calculation combined with the subtractive set pair potential to diagnose and discriminate the unbalanced sub areas. The results show that from 2010 to 2020, the matching of water resources with population and cultivated land area in the Yellow River diversion and received area is in a relatively balanced or balanced level, and the overall water resources spatial equilibrium level is in a relatively balanced or balanced level for a long time; the severe situation of the spatial balance grade of water resources and population shows that Zhengzhou radiates to the surrounding areas, and the spatial balance grade of water resources and cultivated land area shows that the north of Henan Province is worse than that of the south; in 2020, the vulnerable areas of water resources spatial balance include Zhengzhou, Puyang, Xinxiang and Anyang, which are the main control objects of water resources balanced distribution. [ABSTRACT FROM AUTHOR]

BACKGROUND: Patients with pelvic fractures are often accompanied by sacroiliac joint separation, and the most effective surgical treatment for this condition is the placement of sacroiliac screws. At present, the commonly used nail placement method is mainly for the surgeon to place it by hand under fluoroscopy. The accuracy and safety are poor, and the screw position after surgery is closely related to the experience of the surgeon. Orthopedic surgical robot-assisted sacroiliac screw placement is an advanced surgical method recently. However, due to the high cost of robots and limited clinical applications, there are currently many controversies regarding the safety and reliability of the operation. OBJECTIVE: To investigate the clinical effect of robot-assisted sacroiliac screw placement. METHODS: A total of 95 patients with pelvic fractures treated in Foshan Hospital of Traditional Chinese Medicine from January 2018 to January 2020 were reviewed. Among them, 48 patients underwent percutaneous sacroiliac screw placement and 47 patients underwent robot-assisted sacroiliac screw placement. The operation time of sacroiliac screw placement, the number of postoperative complications, Matta score, Majeed score, and Merle D 'Aubigne and Postel score were compared between the two groups. RESULTS AND CONCLUSION: (1) The operation time of the robot-assisted sacroiliac screw placement group was significantly shorter than that in the percutaneous sacroiliac screw placement group (P < 0.05). The incidence of complications was significantly lower in the robot-assisted sacroiliac screw placement group than that in the percutaneous sacroiliac screw placement group (P < 0.05). Five cases of internal fixation failure and three cases of fracture nonunion were found in the percutaneous sacroiliac screw placement group. One case of fracture nonunion was found in the robot-assisted sacroiliac screw placement group. The healing of the above cases was finally achieved by replacing internal fixation. (2) The excellent and good rates of Matta score, Majeed score, and Merle D 'Aubigne and Postel score were significantly higher in the robot-assisted sacroiliac screw placement group than those in the percutaneous sacroiliac screw placement group (P < 0.05). (3) The fluoroscope time was significantly shorter in the robot-assisted sacroiliac screw placement group (7.2± 1.5) s/times than that in the percutaneous sacroiliac screw placement group (25.7±7.6) s/times (P < 0.05). (4) The results showed that the robot-assisted sacroiliac screw placement had the advantages of short operation time and high accuracy. [ABSTRACT FROM AUTHOR]

BACKGROUND: Patients with pelvic fractures are often accompanied by sacroiliac joint separation, and the most effective surgical treatment for this condition is the placement of sacroiliac screws. At present, the commonly used nail placement method is mainly for the surgeon to place it by hand under fluoroscopy. The accuracy and safety are poor, and the screw position after surgery is closely related to the experience of the surgeon. Orthopedic surgical robot-assisted sacroiliac screw placement is an advanced surgical method recently. However, due to the high cost of robots and limited clinical applications, there are currently many controversies regarding the safety and reliability of the operation. OBJECTIVE: To investigate the clinical effect of robot-assisted sacroiliac screw placement. METHODS: A total of 95 patients with pelvic fractures treated in Foshan Hospital of Traditional Chinese Medicine from January 2018 to January 2020 were reviewed. Among them, 48 patients underwent percutaneous sacroiliac screw placement and 47 patients underwent robot-assisted sacroiliac screw placement. The operation time of sacroiliac screw placement, the number of postoperative complications, Matta score, Majeed score, and Merle D 'Aubigne and Postel score were compared between the two groups. RESULTS AND CONCLUSION: (1) The operation time of the robot-assisted sacroiliac screw placement group was significantly shorter than that in the percutaneous sacroiliac screw placement group (P < 0.05). The incidence of complications was significantly lower in the robot-assisted sacroiliac screw placement group than that in the percutaneous sacroiliac screw placement group (P < 0.05). Five cases of internal fixation failure and three cases of fracture nonunion were found in the percutaneous sacroiliac screw placement group. One case of fracture nonunion was found in the robot-assisted sacroiliac screw placement group. The healing of the above cases was finally achieved by replacing internal fixation. (2) The excellent and good rates of Matta score, Majeed score, and Merle D 'Aubigne and Postel score were significantly higher in the robot-assisted sacroiliac screw placement group than those in the percutaneous sacroiliac screw placement group (P < 0.05). (3) The fluoroscope time was significantly shorter in the robot-assisted sacroiliac screw placement group (7.2± 1.5) s/times than that in the percutaneous sacroiliac screw placement group (25.7±7.6) s/times (P < 0.05). (4) The results showed that the robot-assisted sacroiliac screw placement had the advantages of short operation time and high accuracy. [ABSTRACT FROM AUTHOR]

The experiment was to study the effect of dietary methionine levels on expression of SLC12A5 and Musclin mRNA in thigh and breast muscles of Dagu chickens. 240 healthy 16-week-old roosters were selected and randomly divided into three groups, with ten replicates in each group and eight chickens in each replicate. The methionine (Met) level in group H, group M and group L was 0.42%, 0.34% and 0.25%, respectively. Group M was the control group. The trial period was eight weeks. The results showed that the serum Met content in group H was extremely lower than that in group M (P<0.01). The mRNA expression of Musclin in thigh muscle of rooster in group L was extremely higher than that in group H (P<0.01). At the 16th week, the mRNA expression of Musclin in breast muscle of male chicken in group H and group L was extremely lower than that in group M (P<0.01), and that in group H was significantly higher than that in group L (P<0.05). At the 16th week, SLC12A5 mRNA expression in group L was extremely higher than that in group H (P<0.01), and that in group L was significantly lower than that in group M (P<0.05). The SLC12A5 mRNA expression in breast muscle of group H was significantly lower than that of the other two groups at the 24th week (P<0.05). The experiment indicates that dietary Met level can affect serum Met content and protein synthesis of breast muscle and leg muscle, but there is no dose-effect relationship between SLC12A5 gene expression and Met level. [ABSTRACT FROM AUTHOR]

[ Objective] The study aimed to explore the relationship between intestinal bacteria and body size of red claw crayfish ( Cherax quadricarinatus) cultured in ponds, so as to provide reference basis for beneficial intestinal bacteria of shrimp. [Method] The total DNA of the hindgut samples of red claw crayfish cultured in ponds was extracted. The intestinal microorganisms of large individual group( PB group) and small individual group( PS group) were sequenced by 16S rDNA high-throughput sequencing technology, and bioinformatics analysis was carried out [ Result] A total of 1211 species level taxonomic operating units ( OTU s) were detected in PB group,587 OTUs in PS group and 365 common OTU s in PB group and PS group. The results of alpha diversity analysis showed that the Chaol index, Ace index and Shannon index of intestinal microbial diversity of red claw crayfish in PB group were extremely significantly higher than those in PS group ( P < 0. 01). The top ten dominant flora of PB and PS were similar at the phylum, order and genus levels, but there were differences in flora composition and indicator species. At the phylum level, the dominant flora of PB group was Firmicutes, accounting for 42. 87%, while the dominant flora of PS group was Proteus,accounting for 59. 77%. At the order level,the dominant bacteria in PB group was Clostridium,accounting for 41. 25%, while the dominant bacteria in PS group was Enterobacteriaceae, accounting for 57. 01 % . At the genus level, the dominant bacteria in PB group was Clostridium, accounting for 30. 83 %, while the dominant bacteria in PS group was Citrobacter, accounting for 36. 07% . The indicators of PB group were Firmicutes and Planctomycetes, while the indicators of PS group were Proteus. In addition, there were also differences in the metabolic function of flora between PB group and PS group. Except that the abundance of influencing factors and glycan biosynthesis and metab olism in PB group was significantly lower than that in PS group ( P < 0. 05, the same as below), the abundance of other metabolism ( amino acid metabolism, exogenous biodegradation and metabolism, terpene and polyketone metabolism, etc. ) in PB group was significantly higher than that in PS group. [ Conclusion] Compared with the small red claw crayfish, the composition, abundance and metabolic function of intestinal microorganisms of the large red claw crayfish were significantly superior. Therefore, under the same breeding environment and methods, the intestinal microbial composition, abundance and metabolic function of red claw crayfish were one of the internal factors affecting the growth perlormance of red claw crayfish. [ABSTRACT FROM AUTHOR]

In order to study the thermodynamic conditions of iron resources recycled from oxidized molten nickel slag, a calculation model of apeo activity in CaO-FeO-MgO-SiO2 slag system was established，which was used to investigate the influence of binary basicity and MgO content on aFe0 activity in the slag at 1 723 K. The thermodynamic possibilities of oxidation of Fe to Fe3O4 in nickel slag at 1 723 K were analyzed, and the experimental verification was carried out. The results show that the activity o£ aFeo increases first and then decreases with the increase of the basicity, and reaches the maximum value at basicity of 1. 1. When the oxidation temperature was above 1 723 K, the K& of Fe3O4 being further oxidized to Fe2 O3 was less than 0，which could inhibit the formation of Fe2O3. After the molten oxidation process for nickel slag, the iron component is mainly enriched in the magnetite phase, and the average particle size of the magnetite particles is about 52 pm，which paves a way for magnetic separation of the magnetite phase in nickel slags. [ABSTRACT FROM AUTHOR]

目的:探讨自拟降糖方治疗糖尿病患者１５０例的临床效果.方法:选取我院２０１２年９月至２０１５年８月收治的糖尿病患者１５０例,将 患者随机分为对照组和治疗组,每组各有病例７５例.对照组给予常规西药治疗,治疗组服用自拟降糖方,比较两组患者的治疗效果和满意度. 结果:①治疗组治疗有效率为９７．３３％,对照组治疗有效率为７８．６７％,差异有统计学意义(P ＜０．０５).②治疗组治疗满意度为９６．００％,对照组 治疗满意度为７７．３３％,对比有统计学意义(P ＜０．０５). 结论:自拟降糖方在糖尿病的治疗中效果较好,能够改善患者病情,值得临床推广应用. [ABSTRACT FROM AUTHOR]

The graphene oxide/polyaniline/go Id (GO/PANT/Au) ternary composites were prepared successfully by one step method. The graphene oxide prepared by the modified Hummers method was used as the substrate ？ and the aniline monomer was polymerized in situ by using chloroauric acid as the oxidant and the gold source. The morphology and composition analysis showed that chloroauric acid successfully oxidized aniline to polyaniline and was reduced to gold nanoparticles. The electrochemical performance test showed that the specific capacitance of the ternary composites increased first and then decreased with the increase of the amount of oxidant. When the oxidant was acided in an amount of 0.03 mmol, the prepared ternary composite had the largest specific capacitance. The specific capacity is 327 F / g at a current density of 1 A / g in 1 m o l / L H2SO.〗 electrolyte. When the current density reached at 15 A / g, the capacity retention rate was still as high as 81 % compared with initial current density. [ABSTRACT FROM AUTHOR]

The structural model of the cable antenna was obtained by the force density iterative method. The Gaussian distribution was used to simulate the manufacturing error and thermal deformation error. The finite element model of the cable network structure error analysis was constructed. Taking the manufacturing tolerance and working temperature as variables, the influence of structural error on the antenna surface deviation of the cable was analyzed by a specific example. And comparison was made with the profile deviation analyze caused by the analysis of the cable antenna manufacturing error with the Monte Carlo method. The results showed that the method had the accuracy and feasibility of structural error analysis, which could be used to evaluate the impact of the structural error to the antenna profile accuracy. [ABSTRACT FROM AUTHOR]

Stepping motor is an important control element. It is widely used in the automatic control system in the production field. In order to make the students of automatic control specialty master the working characteristics and usage of stepping motor quickly, a new stepping motor control experimental device is developed. The basic requirement for the whole motor control system is to select the suitable microcontroller. The microcontroller of K60 series has a kernel of ARM Cortex-M4. It has many advantages such as high performance, low power consumption, and rich resources on chip. It can meet various function requirements of motor control system. The experimental device is made of magnetic powder brake, reducer, stepping motor, incremental encoder, control circuit and so on. The control circuit is made of K60 minimum system board made by ourselves, power control module, 4x5 matrix keyboard module, torque control module, encoder decoding module, LCD module and so on. In this paper, the designs of hardware and software are introduced in detailed. The experimental device gets good teaching results. [ABSTRACT FROM AUTHOR]

To evaluate the clinical value of 18F-FDG PET/CT for detecting tumor on physical examination people with unexplained elevated serum carcinoembryonic antigen (CEA). Methods A total of 100 physical examination people with unexplained elevated serum levels of CEA in our hospital from June 2010 to December 2014 were involved in the study. All the people were detected with 18F-FDG PET/CT. The pathology, clinical follow ups and conventional medical imaging results were combined to evaluate the value of 18F-FDG PET/CT in diagnosing tumor. The doubling time (DT) of CEA was calculated in the patients who were received more than twice of serum CEA detection. The relationships between serum CEA levels, CEA DT and 18F-FDG PET/CT imaging were analyzed. The receiver operating characteristic (ROC) curve was used to assess the diagnostic efficiency of serum CEA. Results Twenty-seven patients were confirmed with malignant tumor, and the rest of 73 cases were excluded. The 18F-FDG PET/CT reported one false positive and one false negative respectively. The sensitivity, specificity, accuracy, positive and negative predictive values of 18F-FDG PET/CT in diagnosing malignant tumors were 96.3% , 98.6% , 98.0% , 96.3% and 98.6% respectively. The median serum CEA levels were significantly higher in the positive 18F-FDG PET/CT patients than those in the negative patients (Z=5.796, P＜0.05). The prevalence of 18F-FDG PET/CT positive patients was higher with an increase in absolute CEA levels (χ2=37.823, P＜0.05). The median DT of serum CEA levels was shorter in the positive 18F-FDG PET/CT patients than that in the negative patients (Z=4.301，P＜0.05). The positive rates of 18F-FDG PET/CT in patients with 0 d＜DT＜180 d were significantly higher than patients with DT＞180 d and DT＜0 d (χ2=17.472 and 17.325, both P＜0.05). There was no significant difference in the positive rate of 18F-FDG PET/CT between patients with DT＞180 d and patients with DT＜0 d (χ2=0.255，P＞0.05). The area under the curve (AUC) of serum CEA was 0.894, the optimal diagnostic cutoff value was 14.24 μg/L. Conclusion 18FFDG PET/CT is a useful image modality for detecting malignant tumors in patients with unexplained elevated serum CEA, especially for patients with CEA≥14.24 μg/L and increase progressively. [ABSTRACT FROM AUTHOR]