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In order to explore the extraction technology and antioxidant function analysis of polysaccharides from walnut peel, in this study, ethanol/K2HPO4 was used to extract polysaccharides from walnut peel. The extraction rate of polysaccharide was used as the index. On this basis, response surface Box-Behnken coupled genetic algorithm was used to optimize the two-water phase extraction process of walnut peel polysaccharide. The structure was characterized by Fourier Transform Infrared Spectroscopy and its antioxidant activity was studied in vitro. The results showed that the optimal extraction process of polysaccharides from walnut peel by dual-phase technology was as follows: in the dual-phase system, the lower phase K2HPO4 was 16% mass fraction, the dual-phase extraction time was 34 min, and the dual-phase extraction temperature was 28 ℃, so the WPPs yield of 111 mg/g could be achieved. The polysaccharides from walnut peel had characteristic absorption peaks of polysaccharide compounds. The polysaccharides of walnut peel prepared by aqueous two-phase method has significant scavenging effect on ABTS free radicals, the two-phase aqueous system can extract polysaccharides from walnut peel security, inexpensive and effectively, and retain the biological activity of polysaccharides from walnut peel. [ABSTRACT FROM AUTHOR]

Folate is a collective term for tetrahydrofolate (THF) and its derivatives substituted with one carbon unit, playing a crucial role in plant growth and human health. The human body cannot synthesize folate itself, so it must be obtained from the diet. Improving folate content in plants through biofortification is an effective method to address the global folate deficiency problem. This review introduced the structure, functions, the biosynthesis pathways, and the extraction and detection methods of folate. Moreover, it focused on the current status of folate biofortification in different crops, and prospected for global folate biofortfication. [ABSTRACT FROM AUTHOR]

【Objective】 The present paper aimed to explore the physiological and biochemical response of Nymphaea minuta to low light environment, and to provide theoretical basis for seedling breeding and potted flower production management of micro-tropical water lily represented by N.minuta. 【Method】 A completely random experimental design was conducted. The shading treatments were set to 0(total illumination control, CK), 30% (light shading), 50% (medium shading), 70% (heavy shading) respectively by constructing shading nets with different layers. The number of leaves, leaf dry weight, leaf area and other growth indicators were measured by shading treatment for 120 days. The content and composition of photosynthetic pigments, soluble sugar, soluble protein and starch contents were measured by shading treatment for 30, 60, 90 and 120 days respectively. The effects of different shading conditions on leaf growth, photosynthetic pigment content and primary metabolites content of N.minuta were analyzed. 【Result】 During the shading period of 30-120 days, there were no significant differences between the average leaf area, photosynthetic pigment content, and soluble protein content of N.minuta treated with shading rate of 30%, 50% and CK (P＞0.05). After shading treatment for 120 days, the leaf number, average leaf dry weight, specific leaf weight, soluble sugar content, soluble protein content and starch content of N.minuta decreased with increasing shading rate, while total chlorophyll content, chlorophyll a content, chlorophyll b content, chlorophyll a/b content and carotenoid content all increased with increasing shading rate. Comparing with CK, the shading rate of 70% at 120 days treatments, the leaf number, average leaf dry weight and specific leaf weight of N.minuta were significantly decreased by 52.9%, 81.3% and 57.8%, respectively (P＜0.05, the same as below), total chlorophyll content, chlorophyll a content, chlorophyll b content and carotenoid content were significantly increased by 108.7%, 81.6%, 237.5% and 88.2% respectively, chlorophyll a/b content was significantly decreased by 39.1%, soluble sugar content, soluble protein content and starch content were significantly decreased by 43.4%, 56.2% and 53.2% respectively. The results of correlation analysis showed that the leaf number and average leaf dry weight were extremely positively correlated with soluble protein content (P＜0.01, the same as below), and the average leaf dry weight was negatively correlated with total chlorophyll content, chlorophyll a content, chlorophyll b content and carotenoid content. 【Conclusion】 N.minuta grew well in light and medium shading environments with shading rates of 30% and 50%, showing good light adaptive response and regulation ability. These two shading methods can be used for reference in the breeding of micro-tropical waterlily seedlings and potted flower production management. [ABSTRACT FROM AUTHOR]

Objective To explore the relevant risk factors for Refeeding Syndrome (RFS) in ICU patients with sepsis, and to establish a prediction model of RFS based on the selected risk factors. Methods The clinical data of sepsis patients admitted to the ICU of the First Affiliated Hospital of Kunming Medical University from November 2020 to January 2022 were studied retrospectively. Finally, 202 patients were selected according to the inclusion and exclusion criteria. According to the diagnosis criteria of ASPEN Consensus Recommendations (2020) for RFS, the patients were divided into two groups: RFS group (n = 141) and non-RFS group (n = 61) on the basis of whether occur of RFS. The differences of gender, body mass index ( BMI), nutrition risk screening, nutritional way, related critical score, intraabdominal pressure ( IAP), relevant laboratory tests, biochemical indexes and pharmacy were compared between the two groups. The independent risk factors of RFS in ICU patients with sepsis were screened by single factor and multivariate logistic regression analysis, and a prediction model was established according to the results of the analysis. The predictive value of the prediction model for RFS in ICU patients with sepsis was evaluated by drawing the receiver operating characteristic curve (ROC) of the subjects. Results　After analyzing the relevant data of 202 sepsis patients, the results of monofactor logistic regression analysis showed: Body mass index (BMI), albumin (ALB), prealbumin (PA), creatinine-height index (CHI), serum sodium (Na+), serum potassium (K+), serum magnesium (Mg2+), serum phosphorus (P- ), interleukin6 ( IL-6), fasting blood glucose ( FBG), vitamin B1 ( VitB1), glycosylated hemoglobin ( HbA1c), use of diuretics, use of insulin and other indicators were significantly different ( P < 0.05). And multivariate analysis showed that the use of diuretics, BMI, CHI, serum K+, FBG, and VitB1 were independent risk factors for RFS in ICU sepsis patients. Conclusions　Based on the independent risk factors in the multivariate screening, the prediction model expression is established as: L = 1.39×diuretics + 0.15×BMI - 0.14×CHI + 0.75×K+- 0.16×FBG+0.78×VitB1-2.94. The prediction model established by regression analysis has strong consistency, the joint prediction model has better predictive value. [ABSTRACT FROM AUTHOR]

目的 探讨鼻内窥镜下低温等离子翼腭联合鼻丘割治治疗变应性鼻炎 (AR) 的临床疗效及影 响因素。方法 选取 2017 年 1 月至 2020 年 6 月西南医科大学附属中医医院确诊为 AR 且有手术适应证患者 78 例, 对患者术前及术后1年的症状进行评价, 并分析影响术后疗效的相关因素。结果 临床治疗总有效率为 91.0%(71/78)。治疗前后患者临床症状评分比较, 差异有统计学意义 (P <0.05)。78 例患者中, 1例患者术后 出现轻度干眼症状, 1 例患者术后出现流泪症状。单因素分析显示, 有无吸烟史、有无鼻息肉、是否随访为影响 术后疗效的因素。多因素 logistic 回归分析显示, 是否随访是影响术后疗效的主要危险因素。结论 采用鼻内 窥镜下低温等离子翼腭联合鼻丘割治方法治疗 AR 是可行的, 配合术后定期随访, 近期疗效值得肯定。 [ABSTRACT FROM AUTHOR]

A case of young female patients with ovarian malignant Brenner tumor (MBT) was reported. The progress of diagnosis and treatment was summarized. This patient was accompanied by nontypical symptoms of persistent lumbar pain and palpitation. The diagnosis of MBT was confirmed by histopathology, and immunohistochemical markers including the positive GATA3 and CK7, with the tissue of abdominal biopsy. In this paper, the related literatures were also reviewed. The clinical manifestations and imaging manifestations of MBT are unspecific, so the diagnosis is mainly based on histopathological and immunohistochemical markers. The clinical manifestations of patients with advanced stage are diverse, and the functional state of whole body should be brought attention. Two important things are to avoid infection and to improve the life quality as far as possible. [ABSTRACT FROM AUTHOR]

[Objective] Liquid digestate concentrate from membrane bioreactor (MBR) coupled with RO membrane was used to formulate liq uid fertilizer and its fertilizer efficiency and safety on pakchoi ( Brassica chinensis L. ) was investigated. The result is expected to provide a new way for the treatment and resource utilization of liquid digestate. ( Method ) The concentrated liquid digestate from MBR + RO system was re-formulated to meet the standard of liquid fertilizer. The effects of liquid digestate concentrate based fertilizer on the yield and quality of pakchoi as well as soil safety were analyzed under the same amount of nitrogen application and using other types of fertilizers as controls. (Result] Under the same amount of nitrogen application, liquid digestate concentrate based fertilizer could significantly increase the yield, chlorophyll content, soluble sugar content and vitamin C content, and reduce the risk of nitrate and heavy metal accumulation in pakchoi. Conclusion] The liquid digestate concentrate based fertilizer had superior fertilizer efficiency and low risk for pakchoi. The liquid digestate concentrate from MBR + RO system has great potential for fertilizer utilization [ABSTRACT FROM AUTHOR]

Gag-Pol protein is one of the important structural proteins of human immunodeficiency virus (HIV), comprising the basic scaffold proteins and functional enzymes required during the lifecycle of HIV. Currently, the inhibitors targeting different functional domains on Gag-Pol include capsid (CA) inhibitors, protease inhibitors, reverse transcriptase inhibitors, and integrase inhibitors. The CA inhibitors inhibit the maturation of CA or disrupt the assembly of CA, so as to affect the replication of HIV. The primary mechanism of protease inhibitors is to inhibit the protease from cleaving at the cleavage site CA-spacer peptide 1 (SP1). The reverse transcriptase inhibitors block the reverse transcription process of HIV by mimicking the reverse transcription substrates. The integrase inhibitors impact the activity of integrase by targeting the zinc-finger structure at the active center of integrase. This article summarizes the inhibitors targeting HIV Gag-Pol protein and their mechanisms, and reviews the approved dosages and usages of approved patent drugs, so as to provide the references for the future clinical combination therapies and the development of new inhibitors targeting HIV Gag-Pol protein. [ABSTRACT FROM AUTHOR]

Ｉｎ ｏｒｄｅｒ ｔｏ ｏｖｅｒｃｏｍｅ ｔｈｅ ｏｖｅｒｌｙ ｃｏｎｓｅｒｖａｔｉｓｍ ｏｆ ｔｈｅ ｔｒａｄｉｔｉｏｎａｌ ｒｏｂｕｓｔｎｅｓｓ ｍｅｔｈｏｄ ｉｎ ｄｅａｌｉｎｇ ｗｉｔｈ ｔｈｅ ｕｎｃｅｒｔａｉｎｔｙ ｏｆ ｗｉｎｄ ｐｏｗｅｒꎬ ａ ｌｉｇｈｔ ｒｏｂｕｓｔｎｅｓｓ ｂａｓｅｄ ｄｉｓｐａｔｃｈ ｍｏｄｅｌ ｔｈａｔ ｃａｎ ｃｏｎｓｉｄｅｒ ｔｈｅ ｕｎｃｅｒｔａｉｎｔｙ ｏｆ ｗｉｎｄ ｐｏｗｅｒ ｗｉｔｈ ｔｈｅ ｐｒｅｄｉｃｔｉｏｎ ｅｒｒｏｒ ｉｎｆｏｒｍａｔｉｏｎ ｇｉｖｅｎ ｏｎｌｙ ｗａｓ ｐｒｏ￣ ｐｏｓｅｄꎬ ｉｎ ｗｈｉｃｈ ｔｈｅ ａｎｃｉｌｌａｒｙ ｆｒｅｑｕｅｎｃｙ ｓｕｐｐｏｒｔ ｆｒｏｍ ｗｉｎｄ ｐｏｗｅｒ ｗａｓ ｉｎｔｒｏｄｕｃｅｄ ａｎｄ ｔｈｅ ｃｏｎｓｔｒａｉｎｔｓ ａｂｏｕｔ ｔｈｅ ｗｉｎｄ ｆａｒｍ ｒｅｓｅｒｖｅ ｌｉｍｉｔｓ ａｎｄ ｔｈｅ ｃｏｎｖｅｎｔｉｏｎａｌ ｒｅｓｅｒｖｅ ｌｉｍｉｔｓ ｗｅｒｅ ｆｏｒｍｕｌａｔｅｄ. Ｆｉｎａｌｌｙꎬ ｔｈｒｅｅ ｍｅｔｈｏｄｓ ｉｎｃｌｕｄｉｎｇ ｔｈｅ ｎｏｍｉｎａｌ￣ｓｃｅｎａｒｉｏ ｍｏｄｅｌꎬ ｔｈｅ ｃｏｎｖｅｎｔｉｏｎａｌ ｒｏｂｕｓｔ ｍｏｄｅｌ ａｎｄ ｔｈｅ ｐｒｏｐｏｓｅｄ ｌｉｇｈｔ ｒｏｂｕｓｔｎｅｓｓ ｂａｓｅｄ ｍｏｄｅｌ ｗｅｒｅ ｓｙｓｔｅｍａｔｉｃａｌｌｙ ｃｏｍｐａｒｅｄ ｔｏ ｖａｌｉｄａｔｅ ｔｈｅ ｅｆｆｅｃｔｉｖｅｎｅｓｓ ｏｆ ｔｈｅ ｐｒｏｐｏｓｅｄ ｍｅｔｈｏｄꎬ ｕｓｉｎｇ ａ ｍｏｄｉｆｉｅｄ ＩＥＥＥ ３０￣ｂｕｓ ｓｙｓｔｅｍ ｗｉｔｈ ｗｉｎｄ ｐｏｗｅｒ ｉｎｔｅｇｒａｔｅｄ. Ａｎｄ ｔｈｅ ｒｅｓｕｌｔｓ ｓｈｏｗ ｔｈａｔ ｔｈｅ ａｎｃｉｌｌａｒｙ ｆｒｅｑｕｅｎｃｙ ｒｅｇｕｌａｔｉｏｎ ｓｕｐｐｏｒｔ ｃａｎ ｉｍｐｒｏｖｅ ｔｈｅ ｆｅａｓｉｂｉｌｉｔｙ ｏｆ ｔｈｅ ｓｃｈｅｄｕｌｉｎｇ ｓｃｈｅｍｅ. Ｆｕｒｔｈｅｒｍｏｒｅꎬ ｔｈｅ ｌｉｇｈｔ ｒｏｂｕｓｔｎｅｓｓ ｍｅｔｈｏｄ ｉｓ ｍｏｒｅ ｅｃｏｎｏｍｉｃａｌ ｔｈａｎ ｔｈｅ ｔｒａｄｉｔｉｏｎａｌ ｒｏｂｕｓｔ ｍｏｄｅｌ. [ABSTRACT FROM AUTHOR]

Objective To investigate the feasibility, safety, and specific advantages of left bundle branch area pacing (LBBaP) in patients with third-degree atrioventricular block(AVB) compared with right ventricular septal pacing (RVSP). Methods The study was a single-center retrospective case analysis. From September 2020 to January 2022, a total of 56 patients with third-degree AVB and with permanent pacing indications were selected, 26 patients were treated with LBBP and 30 patients with RVSP. The QRS wave width of ECG before and after operation was compared in both groups. Left atrial anteroposterior diameter (LAAD), left ventricular end-diastolic anteroposterior diameter (LVEDD) and left ventricular ejection fraction (LVEF) were measured by cardiac color doppler ultrasound before and three and six months after operation in both groups. The parameters of ventricular electrodes were measured by programmable instrument during operation and three and six months after operation in both groups. The values of N-terminal pro-B-type natriuretic peptide (NT-proBNP) were compared before operation one week after operation and one three and six months after operation in both groups. The postoperative complications of two groups were analyzed. Results QRS wave width in the LBBaP group was narrower than that in the RVSP group after operation, and the difference was statistically significant (P<0.001). The threshold, perception and impedance of the two groups were within the normal range, and there was no increase in threshold in the LBBaP group after operation. The LAAD and LVEDD in the LBBaP group were lower than those in the RVSP group at six months after operation, and LVEF in the LBBaP group was higher than that in the RVSP group at six months after operation, with statistical significance(P<0.05). The value of NT-proBNP in the LBBaP group was lower than that in the RVSP group at three months after operation and the difference was further enlarged at six months after operation. with statistical significance (P<0.05). There was no significant difference in the incidence of complications between the two groups (P>0.05). Conclusion Compared with RVSP, LBBP is a more ideal and safe physiological pacing mode. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of transient receptor potential melastatin 8 （TRPM8） on the immune escape of glioma cells, and to explore the possible mechanism of action. Methods: Real-time fluorescent quantitative PCR was used to detect the expression of TRPM8 gene in human normal glial cell line （SVGp12） and three glioma cell lines （U251, U373, T98G）. The U251 cells were randomly divided into blank group, TRPM8-siRNA group, NC-siRNA group, pcDNA3.1-TRPM8 group and pcDNA3.1-NC group. The liposome method was used to transfect TRPM8-siRNA, NC-siRNA, pcDNA3.1-TRPM8 and pcDNA3.1-NC into U251 cells, real-time fluorescent quantitative PCR and Western blot were used to detect TRPM8 gene and protein expression in cells. CCK-8 method, colony formation experiment and flow cytometry were used to detect cell proliferation, clone formation and apoptosis, lactate dehydrogenase （LDH） release method was used to detect the killing of U251 cells by NK92 cells, ELISA method was used to detect TNF-α, IL-2, IL-6, IFN-γ levels, flow cytometry and Western blot were used to detect the expression of cell regulatory region factor 1 （NCR1） and natural killer factor protein 46 （NKP46）. Results: The expression mRNA levels of TRPM8 in the three glioma cell lines U251, U373 and T98G were significantly higher than those in the normal glial cell line SVGp12, with U251 cells being the highest. Compared with blank group, the relative expressions mRNA and protein of TRPM8 in TRPM8 siRNA group were all down-regulated （P<0.01）, cell activity was decreased（ P<0.05）, the number of cell clone formation was decreased（ P<0.01）, and the rate of apoptodoi sis was increased（ P<0.01）, the killing rate of NK92 cells to U251 cells was increased（ P<0.01）, and the levels of TNF-α, IL-2, IL-6 and IFN-γ in the cell supernatant were decreased （P<0.01）, at the same time, the expressions of NCR1 and NKP46 were also increased significantly （P<0.01）. Compared with blank group, the relative expressions mRNA and protein of TRPM8 in pcDNA3.1-TRPM8 group were up-regulated （P<0.01）, cell activity was increased （P<0.05）, the number of cell clones was increased, and the apoptosis rate was decreased （P<0.01）, the killing rate of NK92 cells to U251 cells decreased （P<0.01）, and the levels of TNF-α, IL-2, IL-6 and IFN-γ in the cell supernatant were increased （P<0.01）, the expressions of NCR1 and NKP46 were decreased （P< 0.01）. Conclusion: Inhibition of TRPM8 expression can regulate the immunosuppressive state in the glioma microenvironment, enhance the ability of NK92 cells to kill U251 cells, and weaken the immune escape ability of tumor cells, which may be related to the regulation of NCR1/NKP46 pathway. [ABSTRACT FROM AUTHOR]

With the increasing number of fuel vehicles, air pollution is becoming increasingly severe. In recent years, some administrative departments has begun to establish zones with specific pollutant emission limits for fuel vehicles and promote people's choice of green transportation methods such as new energy vehicles, low emission zones. In order to understand people's intention to purchase and use new energy vehicles in low emission zones, take fuel commuters as the survey object. Based on self-regulated behavior change stage model, the people's intention to purchase and use new energy vehicles were divided into goal intention stage, behavior intention stage, implementation intention stage, and intention formation stage. Based on each intention stage, auxiliary intervention measures were provided to promote intention improvement. Finally obtain the demand for auxiliary intervention measures at different stages of intention, and establish the before and after phased intervention measures two models for the formation of intention to purchase and use new energy vehicles in low emission zones. The analysis shows that providing a phased intervention model can better explain the formation process of people's intention to purchase and use new energy vehicles in low emission zones. And the changes in the importance factors that affect people's intentions at each stage before and after the intervention of phased measures were analyzed. The results can provide reference and suggestions for some administrative departments to build low emission zones. [ABSTRACT FROM AUTHOR]

Objectives To analyze the correlation of blood lactate concentration in artery，central vein and peripheral vein of patients with septic shock，and to explore the value of blood lactate and lactate clearance rate in different periods of time in evaluating the prognosis of patients with septic shock. Methods A total of 60 patients with septic shock in ICU of building 3 of the First Affiliated Hospital of Kunming Medical University from June 2020 to February 2021 were selected as the research objects. The general data of the patients were collected，and the blood gas parameters of artery，peripheral vein and central vein at admission and 6 h，12 h and 24 h after treatment， as well as arterial blood gas parameters at 48 h and 72 h after the treatment were monitored. First，according to the source of blood samples，they were divided into the artery group，the central vein group and the peripheral vein group. The correlation of blood lactic acid concentration in artery，central vein and peripheral vein was analyzed. Then，according to the 28 day survival of patients，they were divided into the death group and the survival group. The evaluation value of blood lactic acid and lactic acid clearance rate in different periods of early stage on the prognosis of patients was discussed. Results （1） According to Pearson correlation analysis，there was a correlation between arterial lactic acid and central venous lactic acid at ICU admission and 6 h，12 h，24 h after the treatment，with r value between 0.89-0.90 at each time period，P < 0.05；there was a correlation between arterial lactic acid and peripheral venous lactic acid at ICU admission and 6 h，12 h，24 h after treatment，with r value between 0.83-0.87 at each time period，P < 0.05. （2） By drawing ROC curve，the AUC of arterial lactic acid at the initial and 6 h，12 h，24 h，48 h and 72 h after the treatment were 0.64，0.62，0.64，0.72，0.66，0.63， respectively， P < 0.05. The AUC of arterial lactate was the highest at 24 h，with the best cutoff value of 2.42 mmol/L，sensitivity of 0.64 and specificity of 0.66. （3） By drawing the ROC curve，the AUC of arterial lactic acid clearance at 6 h，12 h，24 h，48 h and 72 h were 0.66，0.61，0.74，0.72 and 0.70，respectively， P < 0.05. The AUC of 24 h lactate clearance was the highest，with the best cutoff value of 19.57%，sensitivity of 0.64 and specificity of 0.68. Conclusions （1） There is a correlation between arterial and central venous blood lactic acid levels and between arterial and peripheral venous blood lactic acid levels in patients with septic shock. （2） The arterial lactate and lactate clearance rate 24 hours after the treatment have a good evaluation value for the prognosis of patients with septic shock. [ABSTRACT FROM AUTHOR]

To study Anomobryum auratum in native water and Tortula desertorum in rocky face of two species of mosses in different substrates under the No.1 glacier. Ultrastructural comparison of desedorum at room temperature under ultra low temperature stress and recovery after stress. The mechanism of cold resistance of the mosses in the alpine ice margin was observed at the ultra-low level. After ultrasonic cleaning with ultrasonic analyzer, it was divided into -80 °C ultralow temperature stress, room temperature recovery culture and normal temperature three groups, and ultra-thin sections were prepared by treatment with glularaldehyde, phosphate buffer, citric acid, ethanol, acetone and olher reagents. To observe and analyze the ultrastructure after dyeing. 'Hie results were as follows: The mesophyll of the mosses were intact and dear at room temperature. The ultrastructural changes of mesophyll cells under ultra-low temperature stress were as follows: Most of the mesophyll cells of the two mosses did not have plasmolysis, but the structure of the wall was blurred, the cytoplasm contracted; The organelles were destroyed or even disintegrated; The number of starch granules, lipid droplets and vacuoles were greatly increased. During the recovery at room temperature, the number of mitochondria increased, and each organelle structure was more complete than conditions of ultra-low temperature stress. According to the analysis of the ultrastructure in this paper, these changes are presumed to adapt to the rapid recovery of physiological functions of cells. The ultra-low temperalure stress i -80 C does not completely lose the physiological function of the mosses, and those functions can also be restored. The thickness of mesophyll cell wall in the natural silver carp was 1100-1 300 nm. The thickness of mesophyll cell wall in the rocky spurs was 200-700 nm. The cell wall of the rocky spurs wall was thicker than the natural silver carp. lt is inferred that the resistance lo stress of the thorn wall organelle is also stronger. These two species of miles have strong cold resistance, and their unique cold-resistance mechanism is not only related to the structure and function of plant starch granules and organelles under ultrastructure, but also related to their habitat. [ABSTRACT FROM AUTHOR]

Objective To investigate the effects of treatment with nicorandil after Percutaneous Coronary Intervention (PCI) in patients with Acute Coronary Syndrome (ACS) on inflammation-related markers, and to assess its effects on vascular endothelial function. Methods　Sixty-six ACS patients who underwent PCI in the Department of Cardiovascular Medicine of the Second Affiliated Hospital of Dalian Medical University from August 2022 to January 2023 were used as the study sample, and were divided into the control group and the experimental group according to the method of completely randomized design, with 33 cases in each group. The control group was treated with conventional therapy, and the experimental group was treated with nicorandil. Inflammatory indexes, homocysteine ( Hcy) and adverse reactions in serum were compared between the two groups. Results　After nicorandil treatment, the levels of postoperative inflammation-related factors in the control group were higher than that in the experimental group, and the difference was statistically significant (P < 0.05) ; The levels of Hcy after nicorandil treatment were lower than that in the control group, and the difference was statistically significant (P < 0.05) ; and the rate of adverse reactions in the experimental group was higher than that in the control group, and there was no statistical difference (P > 0.05). Conclusion　Nicorandil application in elderly ACS patients after PCI has a definite efficacy, can optimize the vascular-related inflammatory indexes, reduce homocysteine levels to improve coronary vascular endothelial function, and is suitable for further promotion. [ABSTRACT FROM AUTHOR]

With the rise of soilless cultivation technology, the substrate market has developed rapidly and the demand has increased day by day. The development and application of substrates have become an important research field. Peat, as the main substrate raw material, not only has limited resources, but its large-scale mining will also damage the ecological environment. This article reviews the research status of sphagnum moss and the basic performance indicators as a substrate material, and comparatively analyzes the connections and differences between peat moss, peat and other substrate materials, and uses it as a matrix based on the production principles of standard seedling substrates and cultivation substrates. An in-depth discussion is conducted to look forward to the development prospects of peat moss as a substrate, aiming to provide reference for the restoration of peat resources, protection of the ecological environment and the matrix utilization of peat moss. [ABSTRACT FROM AUTHOR]

[Objective] The Unigene produced by leaf transcriptome of Nymphaea colorata and N.micrantha and the whole genome sequence of N.colorata were analyzed according to SSR loci characteristics. It provided basic data for the germplasm resource identification, genetic diversity analysis and genetic linkage map construction of water lily plants.[Method] Based on the leaf transcriptome data of N.colorata and N.micrantha which were acquired previously in the laboratory and the published genome of N.colorata,MISA software was used to search SSR loci and analyze the frequency, length and primitive types of SSR.[Result] 38 998 SSR loci were found among the 114 762 Unigenes sequences obtained from the transcriptome of N.colorata and N.micrantha.The SSR site frequency was 33.98%.Among them, 30 124 SSR loci belong to complete types. The total length of the SSR motif sequence was 465 550 bp, and had a total average of 21.65 bp.249 029 SSR sites were searched in the N.colorata genome, with an average distribution frequency of 609.0/Mb, including 163 265 intact SSR sites; the total length of SSR motif sequence was 2 775 181 bp, and 27.25 bp, accounted for 0.68% of N.colorata genome size. In the transcriptome and genome, the SSR loci were mainly dinucleotide and single nucleotide repeats, which respectively accounted for 48.87%,41.03% and 51.70%,43.37% of the total SSR. Among dinucleotide repeat motifs, AG/TC and AT/TA types were higher and both much higher than other types of repeat motifs. The number of repetitions of each type in the SSR primitive was mainly 5 to 11 times, of which 10 times were the most. Twelve pairs of SSR primers with good amplification and high polymorphism were selected based on fluorescence capillary electrophoresis from synthetic 144 pairs of SSR primers.[Conclusion] Low-level primitive types are abundant in the SSR of N.colorata and N.micrantha transcriptome and genome, which has great potential for developing and screening SSR primers with high polymorphism. The selected 12 pairs with good amplifications and high polymorphism of SSR primers can be used to study the identification of germplasm resources, genetic diversity analysis and genetic construction of plant chain map. [ABSTRACT FROM AUTHOR]

Objective T o explore the part mechanism of Buzhong Yiqi Recipe in interfering with laryngeal squamous cell carcinoma (LSCC) cell lines. Methods Using literature review and bio information analysis methods, the main taget gene proteins were screened out: miRNA 138 5p, S0X9, YAPl, FOX Cl, combined wit h LSCC cell line culture in vitro and normal saline (control group ), Chinese medicine compound ( Buzhong Yiqifang granule freeze dried powder, traditional Chinese medicine compound group ) control intervention experiment, using Real time fluorescent quantitative polymerase chain reaction and Western blotting [Glyceral dehyde3 phosphate dehydrogenase (GAPDH) as standard internal reference control], the ex pression differences of the above mentioned screening indicators were tested and verified respectively. Results Compared with t he control group, the expression level of miRNA 138 5p in the Chinese medicine compound group was higher, and the difference was statistically significant (P < O. OS). T he expression levels of SOX9 and FOXC1 protein in the traditional Chinese medicine compound group were lower than t hose in the control group, and t he differences we re statistically significant (PO. OS) . Conclusion Buzhong Yiqi Recipe (Traditional Chinese Medicine Classics) can down regulate t he expression of SOX9 and FOXC1 protein thro ugh miRNA 138 5p, and inhibit LSCC cell line. [ABSTRACT FROM AUTHOR]

Rice husk carbon was prepared by hydrothermal method from rice husk, and used to adsorb methylene blue from printing and dyeing wastewater. Rice husk carbon was characterized by X-ray diffraction(XRD), Scanning Electron Microscope(SEM), Thermo Gravimetric(TG), Fourier Transform Infrared Spectrometer(FT-IR), and the effects of hydrothermal temperature and hydrothermal time, respectively, on the physicochemical properties and adsorption properties of methylene blue in rice husk carbon were investigated. The results showed that hydrothermal temperature was the important factor to affect the adsorption performance of rice husk carbon. In this study, when the hydrothermal time was 4 h and the hydrothermal temperature was 180-220 ℃, the removal rate of methylene blue in rice husk carbon was more than 90% and the adsorption capacity of rice husk carbon to methylene blue was more than 6.27 mg/g. Among them, the hydrothermal temperature was 200 °C and the hydrothermal time was 8 h(RH-200-8), the structure of rice husk carbon was intact and the yield of rice husk carbon was 65.20%. Besides, the removal rate and adsorption capacity of methylene blue in rice husk carbon were 93.04% and 6.62mg /g in the same condition, respectively. The phenomenon was due to the cellulose was not completely decomposed, the pore structure of rice husk carbon was better and the pore diameter of rice husk carbon was moderate when the hydrothermal temperature was 180-220 °C. In addition, the structure of rice husk carbon was conducted to the methylene blue molecule diffusion and adsorption, and rice husk carbon has more oxygen-containing functional groups with more adsorption active points, so the adsorption performance of rice husk carbon on methylene blue was better. When the hydrothermal temperature was greater than 220 °C, with the increase of hydrothermal temperature, the cellulose would be accelerated decomposition, the oxygen-enriched functional groups were decomposed quickly, the adsorption activity points of rice husk carbon were reduced, the rice husk carbon structure was also changed and the specific surface area was increased, but most probable pore sizes of rice husk carbon were decreased, the phenomenon was not conducted to the methylene blue molecule diffusion in rice husk carbon, so the removal rate and adsorption ability of methylene blue in rice husk carbon decreased. The experiment of rice husk carbon(RH-200-8) regeneration and recycling showed that the removal rate of methylene blue in rice husk carbon decreased slightly with the number of rice husk carbon regeneration increased, and the maximum methylene blue removal rate of RH-220-8-3(Rice husk charcoal use 3rd) reached 82.20% (10 h). [ABSTRACT FROM AUTHOR]

Objective To summarize the allergic reaction and nursing measures of clinical commonly used non ionic iodine contrast agent. Methods Between May 2012 and December 2015， 170 cases of allergic reactions occurred within 24 hours after the injection of nonionic iodine contrast agent. The factors， such as the type， concentration and flow rate of the contrast agents， and the nursing measures were analyzed. Results The incidence of total adverse reactions of iopromide， iohexol， and iopamidol were 0.38% and 0.35%， 0.44%， respectively. The total adverse reactions rate of iopamidol was slightly higher than the iopromide and iohexol， the three drugs had no statistical difference （P > 0.05） . The incidence of laryngeal edema and allergic shock in iopromide group were seven in one hundred thousand and three in thousand， rare adverse reactions. Conclusion A small part of patients who used non ionic iodine contrast agent may appeare allergic reactions in different degrees， so we should be careful， strengthen the care， gurrantee the clinical drug safety of iodine contrast agent. [ABSTRACT FROM AUTHOR]

Objective To evaluate the clinical application of high quality for super-selective renal artery embolization （SRAE） in treating bleeding after percutaneous nephrolithotomy （PCNL）. Methods 134 patients received percutaneous nephrolithotomy were divided into control group （67 patients） and observation group （67 patients）. All of patients with serious bleeding after PCNL were given SRAE in the Second Affiliated Hospital of Kunming Medical College from June 2010 to June 2015. At the same time, we gave high quality nursing to observation group. The patients in control group received routine nursing. The effect of nursing was observed. Results The degree of hematuria disappear of the patients in observation group was higher than that in control group （P ＜ 0.05）. There were fewer complications in observation group. In the sixth month after discharge, none of them had obvious renal impairment. No recurrence of hematuria, pus kidney and urinary cyst was tested. All cases were satisfied with the treatment. Conclusion It's the key to prevent serious complications and cure successfully with effective and timely supervision and high quality nursing care during the perioperation of SRAE in treating bleeding after PCNL. [ABSTRACT FROM AUTHOR]