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This paper revisits the main thoughts of the Filipino historian and social critic Renato Constantino on the role of education in the formation of a neocolonial and postcolonial consciousness. It suggests that Constantino's critical stance towards education embodies a type of philosophizing about education that centers on the problematization of the language of instruction, on the rendering of voice to those in the margins of Philippine society, and on taking a critical stance about education itself. It suggests further that this form of philosophizing about education and its instrumentalities is prospective of a Freirean liberating pedagogy that is inherently nonneutral, critical, dialogical, praxical, and transformative. [ABSTRACT FROM AUTHOR]

Objective: To evaluate the effectiveness of mindfulness therapy as an adjunct to pharmacological treatment for improving blood pressure (BP) control in hypertensive adult patients at a health center in Lima, Peru. Method: This study employed a quasi‐experimental design with a quantitative and longitudinal cohort approach. The participants included 50 adults who were diagnosed with hypertension and who regularly attended the health center in the Villa El Salvador district of Lima, Peru. The data were collected using three instruments: the Depression Anxiety Stress Scale (DASS 21), validated in Peru; a sociodemographic questionnaire; and a BP recording sheet. The Wilcoxon test was utilized to assess the effectiveness of mindfulness therapy on BP control, while the nonparametric Friedman test was used to evaluate the effectiveness of mindfulness therapy considering confounding factors. Results: Therapy resulted in favorable outcomes for BP control. After eight sessions, the average systolic BP decreased from 122.8 to 115.0 mm Hg (a reduction of 7%), and the average diastolic BP decreased from 78.1 to 69.9 mm Hg (a reduction of 11%). Conclusions: Mindfulness is an effective complementary therapy for controlling BP in hypertensive adults. It also helps reduce confounding factors such as stress, anxiety, and depression. [ABSTRACT FROM AUTHOR]

Biological mechanisms related to cancer development can leave distinct molecular fingerprints in tumours. By leveraging multi-omics and epidemiological information, we can unveil relationships between carcinogenesis processes that would otherwise remain hidden. Our integrative analysis of DNA methylome, transcriptome, and somatic mutation profiles of kidney tumours linked ageing, epithelial–mesenchymal transition (EMT), and xenobiotic metabolism to kidney carcinogenesis. Ageing process was represented by associations with cellular mitotic clocks such as epiTOC2, SBS1, telomere length, and PBRM1 and SETD2 mutations, which ticked faster as tumours progressed. We identified a relationship between BAP1 driver mutations and the epigenetic upregulation of EMT genes (IL20RB and WT1), correlating with increased tumour immune infiltration, advanced stage, and poorer patient survival. We also observed an interaction between epigenetic silencing of the xenobiotic metabolism gene GSTP1 and tobacco use, suggesting a link to genotoxic effects and impaired xenobiotic metabolism. Our pan-cancer analysis showed these relationships in other tumour types. Our study enhances the understanding of kidney carcinogenesis and its relation to risk factors and progression, with implications for other tumour types. Synopsis: Integrative analysis of multi-omics and epidemiological data implicated ageing, epithelial-mesenchymal transition (EMT), and xenobiotic metabolism as biological mechanisms driving clear cell renal cell carcinoma (ccRCC). Cellular mitotic ageing is a major source of variance between ccRCC tumours, with faster ticking mitotic clocks (epiTOC2, SBS1, and telomere length), genomic instability and PBRM1 and SETD2 mutations related to tumour progression. There is a relationship between BAP1 driver mutations, the epigenetic activation of EMT related genes (IL20RB and WT1), tumour immune infiltration, and worse survival outcomes. Epigenetic silencing of GSTP1, especially in smokers, points to impaired xenobiotic metabolism and increased genotoxic risk in ccRCC tumours. These biological mechanisms were also observed across other cancer types, highlighting broader implications for tumour progression. Integrative analysis of multi-omics and epidemiological data implicated ageing, epithelial-mesenchymal transition (EMT), and xenobiotic metabolism as biological mechanisms driving clear cell renal cell carcinoma (ccRCC). [ABSTRACT FROM AUTHOR]

Background/Objectives: Cartilage injuries and osteoarthritis are prevalent public health problems, due to their disabling nature and economic impact. Mesenchymal stromal cells (MSCs) isolated from different tissues have the immunomodulatory capacity to regulate local joint environment. This translational study aims to compare cartilage restoration from MSCs from the synovial membrane (SM) and dental pulp (DP) by a tissue-engineered construct with Good Manufacturing Practices. Methods: A controlled experimental study was conducted on fourteen miniature pigs, using scaffold-free Tissue Engineering Constructs (TECs) from DP and SM MSCs, with a 6-month follow-up. Total thickness cartilage defects were created in both hind knees; one side was left untreated and the other received a TEC from either DP (n = 7) or SM (n = 7). An MRI assessed the morphology using the MOCART scoring system, T2 mapping evaluated water, and collagen fiber composition, and histological analysis was performed using the ICRS-2 score. Results: The untreated group had a mean MOCART value of 46.2 ± 13.4, while the SM-treated group was 65.7 ± 15.5 (p < 0.05) and the DP-treated group was 59.0 ± 7.9 (n.s.). The T2 mapping indicated a mean value of T2 of 54.9 ± 1.9 for native cartilage, with the untreated group at 50.9 ± 2.4 (p < 0.05). No difference was found between the T2 value of native cartilage and the treated groups. The ICRS-2 mean values were 42.1 ± 14.8 for the untreated group, 64.3 ± 19.0 for SM (p < 0.05), and 54.3 ± 12.2 for DP (n.s.). Conclusion: MRI and histological analysis indicated that TEC treatment led to superior cartilage coverage and quality compared to the defect group. TECs from SM demonstrated better results than the defect group in the histological assessment. [ABSTRACT FROM AUTHOR]

The time-fractional coupled Korteweg–De Vries equations (TFCKdVEs) serve as a vital framework for modeling diverse real-world phenomena, encompassing wave propagation and the dynamics of shallow water waves on a viscous fluid. This paper introduces a precise and resilient numerical approach, termed the Conformable Hyperbolic Non-Polynomial Spline Method (CHNPSM), for solving TFCKdVEs. The method leverages the inherent symmetry in the structure of TFCKdVEs, exploiting conformable derivatives and hyperbolic non-polynomial spline functions to preserve the equations' symmetry properties during computation. Additionally, first-derivative finite differences are incorporated to enhance the method's computational accuracy. The convergence order, determined by studying truncation errors, illustrates the method's conditional stability. To validate its performance, the CHNPSM is applied to two illustrative examples and compared with existing methods such as the meshless spectral method and Petrov–Galerkin method using error norms. The results underscore the CHNPSM's superior accuracy, showcasing its potential for advancing numerical computations in the domain of TFCKdVEs and preserving essential symmetries in these physical systems. [ABSTRACT FROM AUTHOR]

Special functions have been widely used in fractional calculus, particularly for addressing the symmetric behavior of the function. This paper provides improved delta Mittag–Leffler and exponential functions to establish new types of fractional difference operators in the setting of Riemann–Liouville and Liouville–Caputo. We give some properties of these discrete functions and use them as the kernel of the new fractional operators. In detail, we propose the construction of the new fractional sums and differences. We also find the Laplace transform of them. Finally, the relationship between the Riemann–Liouville and Liouville–Caputo operators are examined to verify the feasibility and effectiveness of the new fractional operators. [ABSTRACT FROM AUTHOR]

Fractional integrals and inequalities have gained a lot of attention in recent years. By introducing innovative analytical approaches and applications, and by applying these approaches, numerous forms of inequalities have been examined. In this paper, we establish new identity for the twice differentiable function where the absolute value is convex. By utilizing this identity, numerous Corrected Euler-Maclaurin-type inequalities are developed for the Caputo-Fabrizio fractional integral operator. Based on this identity, the Corrected Euler-Maclaurin-type inequalities for $s$ s -convex function are obtained. By employing well-known inequalities such as Hölder's and Power -Mean, we are introduced several new error bounds and estimates for Corrected Euler-Maclaurin-type inequalities. Additionally, special cases of the present results are applied to obtain the previous well-known results. [ABSTRACT FROM AUTHOR]

We establish new conformable fractional Hermite-Hadamard (H–H) Mercer type inequalities for harmonically convex functions using the concept of support line. We introduce two new conformable fractional auxiliary equalities in the Mercer sense and apply them to differentiable functions with harmonic convexity. We also use Power-mean, Hölder's and improved Hölder inequality to derive new Mercer type inequalities via conformable fractional integrals. The accuracy and superiority of the offered technique are clearly depicted through impactful visual illustrations. We also use our technique to derive new estimates for hypergeometric functions and special means of real numbers that are more precise than existing ones. Some applications are provided as well. Our results generalize and extend some existing ones in the literature. [ABSTRACT FROM AUTHOR]

The author calls for comprehensive training and support to fight workplace violence in the critical care setting. Topics discussed include the role of nurses in enhancing their safety and of their patients, the U.S. Occupational Safety and Health Administration's proposed standard requiring health care employers to develop programs for workplace violence prevention, and evidence-based approaches to violence assessment at the bedside.

Stem cell transplantation into seminiferous tubules of recipient testis could become a tool for fertility restoration, genetic improvement, or conservation of endangered species. Spermatogonial stem cells (SSCs) are primary candidates for transplantation; however, limited abundance, complexity for isolation and culture, and lack of specific markers have limited their use. Mesenchymal stromal/stem cells (MSCs) are multipotent progenitors that are simple to isolate and culture and possess specific markers for identification, and immune evasive and migratory capacities. The objective of the present study was to evaluate the potential for survival and colonization in seminiferous tubules of two different concentrations of bovine fetal adipose tissue-derived MSCs (AT-MSCs), native of pre-induced, and to compare the fate of bovine adult peripheral blood-derived MSCs (PB-MSCs) and SSCs after allogenic transplantation in testis of recipient bulls. In experiment 1, AT-MSCs at two concentrations (1x107 and 2x107; n = 3) or pre-exposed to 2 μM testosterone and 1 μM retinoic acid (RA) for 14 days (n = 5) were evaluated. In experiment 2, adult PB-MSCs and SSCs (4x107 cells each) pre-exposed to Sertoli cell conditioned media (SCs/CM; n = 4) for 14 days were compared. Each cell type was separately labelled with PKH26 and then transplanted into testes of 8-month-old recipient bulls. Four weeks (Exp. 1) and two weeks (Exp. 2) after transplantation, testicular tissue was processed for confocal microscopy detection of PKH26-positive cells. Mean number of PKH26-positive cells were higher (P < 0.05) in testis transplanted with 2x107 AT-MSCs in the proximal (6.7 ± 3.7) and medial (6.6 ± 3.2) sections compared to testis transplanted with 1x107 AT-MSCs (proximal: 1.9 ± 1; medial: 1.9 ± 1) sections or pre-induced AT-MSCs (proximal: 4.7 ± 5.6; medial: 3.8 ± 4.1). In Exp. 2, mean number of PKH26-positive SSCs in medial testicular section (22.5 ± 1.3) were higher (P < 0.05) compared to respective section in PB-MSCs group (17 ± 4.2). Thus, in vivo data indicates that a higher number of transplanted AT-MSCs resulted in more cells surviving and colonizing seminiferous tubules; however, pre-induction with testosterone and RA did not improve these capacities. SSCs displayed a greater capacity for survival and colonization in recipient seminiferous tubules; however, PB-MSCs were observed in all sections of testis after two weeks of transplantation. • MSCs are multipotent precursors with immune evasive capacity. • Bovine MSCs colonization capacity was evaluated in seminiferous tubules. • AT-MSCs were able to survive and colonize in allogenic recipient testis. • PB-MSCs were observed in all sections of testis after two weeks of transplantation. • SSCs had greater capacity for survival and colonization in recipient testis. [ABSTRACT FROM AUTHOR]

Arrangements of piezoelectric transducers, such as PZT (lead zirconate titanate), have been widely used in numerous structural health monitoring (SHM) applications. Usually, when two or more PZT transducers are placed close together, significant interference, namely crosstalk, appears. Such an effect is usually neglected in most SHM applications. However, it can potentially be used as a sensitive parameter to identify structural faults. Accordingly, this work proposes using the crosstalk effect in an arrangement of PZT transducers modeled as a multiconductor transmission line to detect structural damage. This effect is exploited by computing an impedance matrix representing a host structure with PZTs attached to it. The proposed method was assessed in an aluminum beam structure with two PZTs attached to it using finite element modeling in OnScale® software to simulate both healthy and damaged conditions. Similarly, experimental tests were also carried out. The results, when compared to those obtained using a traditional electromechanical impedance (EMI) method, prove that the new approach significantly improved the sensitivity of EMI-based technique in SHM applications. [ABSTRACT FROM AUTHOR]

Background/Objectives: Oreganum vulgare essential oil (OEO) is safe, effective, multifunctional, and widely used. This study aimed to evaluate OEO's chemical composition and antimicrobial activity in vitro against S. aureus and E. coli. Methods: The composition of OEO was determined by gas chromatography–mass spectrometry (GC-MS). Results: Compounds included monoterpenes with known antimicrobial activity, such as 2-menthen-1-ol (36.33%), linalyl acetate (9.26%), terpinene-4-ol (9.01%), 4-thujanol (6.33%), menthen (5.81%), sabinene (5.18%), and carvacrol methyl ether (5.14%). Conclusions: OEO had a strong antimicrobial activity with a minimum inhibitory concentration (MIC) of 1.90 mg/mL for S. aureus and 0.49 mg/mL for E. coli after 18 h incubation. The minimum bactericidal concentration (MBC) was 7.9 mg/mL against S. aureus and 0.99 mg/mL against E. coli. Thus, OEO could be used as a natural antimicrobial against S. aureus and E. coli infections. [ABSTRACT FROM AUTHOR]

Changes in host species richness can alter infection risk and disease levels in multi‐host communities. I review theoretical predictions for direct and environmental transmission pathogens about the effects of host additions (or removals) on three commonly used disease metrics: the pathogen community reproduction number R0 and infection prevalence and infected density in a focal host. To extend this prior work and explain why predictions differ between metrics, I analyze Susceptible Infected‐Recovered‐type models of an environmentally transmitted pathogen and multiple host species that compete for resources. Using local sensitivity analysis, I show how trait‐mediated and density‐mediated indirect effects drive each metric's response to variation in an added host's ability to transmit a pathogen, the added host's density, and the pathogen transmission mechanism. For each disease metric, the responses are typically predicted by the added host's ability to transmit the pathogen when interspecific competition is weak whereas the responses can be altered by shifts in host densities when interspecific competition is strong. In addition, the three metrics often respond in the same direction. However, the metrics can respond in different directions for three reasons: (1) differences between the ability of exposed individuals to transmit the pathogen over the length of time the individuals are infected (i.e., host competence) and a host population's instantaneous net rate of production of infectious propagules; (2) strong density‐mediated feedbacks driven by disease‐induced mortality; and (3) host additions or removals cause large changes in focal host density via competition or disease‐induced mortality. This study extends and unifies prior theoretical studies, and helps identify the rules governing the context‐dependent relationships between host species richness and the three metrics of disease. [ABSTRACT FROM AUTHOR]

Objectives Our objective was to investigate the prevalence of small intestinal atresia and Hirschsprung's disease (HD) in infants with Down syndrome (DS) and its impact on outcomes. Study Design We analyzed the National Inpatient Sample dataset. We included infants with DS, small intestinal atresia, HD, and the concomitant occurrence of both conditions. Regression analysis was used to control clinical and demographic variables. Results A total of 66,213,034 infants were included, of whom, 99,861 (0.15%) had DS. The concomitant occurrence of small intestinal atresia and HD was more frequent in infants with DS compared with the general population, adjusted odds ratio (aOR): 122, 95% confidence interval (CI): 96–154, (p < 0.001). Infants with DS and concomitant small intestinal atresia and HD had higher mortality compared with those without these conditions, aOR: 8.59, 95% CI: 1.95–37.8. Conclusion Infants with DS are at increased risk of concomitant small intestinal atresia and HD, and this condition is associated with increased mortality. Key Points Infants with Down syndrome are at increased risk of congenital GI anomalies. Infants with Down syndrome are at increased risk of necrotizing enterocolitis. Increased mortality in Down syndrome infants with concomitant small intestinal atresia and Hirschsprung's disease. [ABSTRACT FROM AUTHOR]

In Brazil and around the world, self-medication is practiced for various health problems, including obesity. The use of these drugs can cause several health problems, increasing their severity when nursing professionals resort to this resource to lose weight. The objective of the study was to analyze self-medication for weight loss among nursing professionals, in addition to identifying which medications were used. 303 nursing professionals were interviewed, from 01/21/2022 to 04/24/2022, after approval by the CEP. Among the professionals who stated that they resort to this practice for weight loss purposes, 25% are nurses and 75% nursing technicians. The most cited drugs were sibutramine 24.62%, herbal medicines 21.54% and bupropion 18.46%. The results reveal a serious public health problem among these professionals, who are responsible for caring for and guiding the population, making it necessary to adopt public policies aimed at nursing care. [ABSTRACT FROM AUTHOR]

We prove that an equation holds for differentiable convex functions, and this result has been derived using conformable integrals. With the help of this equality, several parameterized inequalities are established by using the conformable fractional integrals. Namely, we show that our main inequalities reduce to Ostrowski-, Hermite–Hadamard-, Simpson-, and Bullen-type inequalities which are proved in earlier published papers. More precisely, some inequalities are acquired by taking advantage of the convexity, the Hölder, and the power mean inequalities. Finally, examples are given to illustrate the investigated results. [ABSTRACT FROM AUTHOR]

We address the issue of inequivalent Fock representations in quantum field theory in a curved homogenous and anisotropic cosmological background, namely Kantowski–Sachs spacetime, which can also be used to describe the interior of a nonrotating black hole. A family of unitarily equivalent Fock representations that are invariant under the spatial isometries and implement a unitary dynamics can be achieved by means of a field redefinition that consists of a specific anisotropic scaling of the field configuration and a linear transformation of its momentum. Remarkably, we show that this kind of field redefinition is in fact unique under our symmetry and unitary requirements. However, the physical properties of the Hamiltonian dynamics that one obtains in this way are not satisfactory, inasmuch as the action of the Hamiltonian on the corresponding particle states is ill defined. To construct a quantum theory without this problem, we need a further canonical transformation that is time- and mode-dependent and is not interpretable as an anisotropic scaling. The old and new Fock representations, nevertheless, are unitarily equivalent. The freedom that is introduced when allowing for this further canonical transformation can be fixed by demanding an asymptotic diagonalization of the Hamiltonian and a minimal absorption of dynamical phases. In this way, the choice of vacuum and the associated Fock representation are asymptotically determined. [ABSTRACT FROM AUTHOR]

This article analyzes the relationship between emotional intelligence (EI) and academic performance in university students, particularly in virtual education contexts, and examines how EI functioned as an adaptation factor during the COVID-19 pandemic. The methodology was based on a document review, with scientific articles on the interaction between emotional skills and performance in online learning environments selected through the PRISMA method. The results indicate that EI plays a fundamental role in managing emotions such as stress and anxiety, factors that significantly impact academic performance in the virtual modality. It was found that students with high levels of EI demonstrated greater adaptability, emotional stability, and self-regulation, positively contributing to their academic achievements despite the challenges of remote learning. The conclusions highlight the importance of implementing EI development programs in universities, for both students and instructors, with the aim of strengthening emotional well-being and improving academic outcomes in higher education. EI not only facilitates the learning process but also enhances resilience and intrinsic motivation, which are essential in a context of uncertainty and constant change. [ABSTRACT FROM AUTHOR]

The article studies the influence of attachment on early childhood socio-emotional development, examining the relevance of parenting practices and the family environment in forming secure attachment, as well as interventions aimed at improving attachment quality in vulnerable populations. The methodology is based on a literature review of recent studies addressing the impact of secure attachment on emotional regulation and social skills, as well as child adaptation and resilience in diverse family contexts. Key findings highlight that secure attachment fosters emotional and social competencies, contributing to greater empathy and stress management. Additionally, responsive parenting practices and a stable family environment reinforce secure bonding, while early interventions in vulnerable contexts show long-term benefits in children's mental health and emotional development. In conclusion, secure attachment is fundamental to child well-being, and its promotion in early years can reduce future emotional issues, enhancing adaptability and overall well-being. [ABSTRACT FROM AUTHOR]

The development of socio-emotional skills in students represents a crucial activity for the acquisition of competencies that allow for the formation of comprehensive well-being in the future. The present research aims to develop a pedagogical strategy for the development of socioemotional skills in 8th year students of basic general education of the fiscal educational unit Lcda. Águeda González Quiñonez. A mixed-type development study was carried out, combining qualitative and quantitative approaches. Observation and interviews were used to diagnose the current state of comprehensive training in students. The data analysis revealed significant deficiencies in skills such as self-awareness, emotion management, empathy, and collaboration among students. Based on these findings, a comprehensive pedagogical strategy was designed that includes emotional reflection activities, group dynamics, conflict resolution workshops, and assertive communication exercises. A single-group pre-experimental design was applied, where notable improvements in students' self-awareness and ability to manage their emotions and work as a team could be seen, highlighting the effectiveness of pedagogical approaches that integrate social-emotional learning into the school curriculum. These results suggest that the adoption of specific pedagogical strategies for social-emotional development can have a positive impact on students' well-being and academic performance. [ABSTRACT FROM AUTHOR]

This article reports on findings from a social design‐based study conducted with an intergenerational group of youth, educators and researchers participating in the Learning to Transform (LiTT) Gaming Lab. We advance the notion of AlgoRitmo Literacies, to highlight the ingenuity of youth and educators as they used a tool called Character AI to author lore emerging within a virtual city called LiTT City. We conceptualize AlgoRitmo—a play on the word algorithm—as part inquiry and reflection (the algo or "something" of AI tools), and part action and future‐oriented (ritmo as in movement). Inspired by cosmogonies influenced by Coyolxauhqui, the fragmented Aztec moon goddess, this paper illustrates how young people reconfigure AI artifacts, reshape relationships with AI‐governed non‐playable characters, and repurpose AI tools to envision alternative futures and identities. In identifying AlgoRitmo Literacies, we provide examples of how ChicanX communities subvert ideologies embedded in AI through creative and ingenious interventions in video games and the construction of cyborg Chicanx subjectivities. This paper offers implications for how educators across content areas can leverage gaming, and AI tools, toward consequential literacy development. [ABSTRACT FROM AUTHOR]

Microfluidic systems offer controlled microenvironments for cell-to-cell and cell-to-stroma interactions, which have precise physiological, biochemical, and mechanical features. The optimization of their conditions to best resemble tumor microenvironments constitutes an experimental modeling challenge, particularly regarding carcinogenesis in the central nervous system (CNS), given the specific features of the blood–brain barrier (BBB). Gel-free 3D microfluidic cell culture systems (gel-free 3D-mFCCSs), including features such as self-production of extracellular matrices, provide significant benefits, including promoting cell–cell communication, interaction, and cell polarity. The proposed microfluidic system consisted of a gel-free culture device inoculated with human brain microvascular endothelial cells (HBEC5i), glioblastoma multiforme cells (U87MG), and astrocytes (ScienCell 1800). The gel-free 3D-mFCCS showed a diffusion coefficient of 4.06 × 10−9 m2·s−1, and it reconstructed several features and functional properties that occur at the BBB, such as the vasculogenic ability of HBEC5i and the high duplication rate of U87MG. The optimized conditions of the gel-free 3D-mFCCS allowed for the determination of cellular proliferation, invasion, and migration, with evidence of both physical and biochemical cellular interactions, as well as the production of pro-inflammatory cytokines. In conclusion, the proposed gel-free 3D-mFCCSs represent a versatile and suitable alternative to microfluidic systems, replicating several features that occur within tumor microenvironments in the CNS. This research contributes to the characterization of microfluidic approaches and could lead to a better understanding of tumor biology and the eventual development of personalized therapies. [ABSTRACT FROM AUTHOR]

Simple Summary: The in vitro culture of mammalian embryos lacks different embryotrophic factors that are present in the female reproductive tract and are essential for correcting embryonic development. It has been reported that supplementation with oviductal fluid (OF) and uterine fluid (UF) during the in vitro culture of mammalian embryos produced by IVF has a positive effect on their development and quality. We investigated the effect of OF–UF supplementation on the balance of reactive oxygen species (ROS), glutathione (GSH), and the development of cloned and parthenogenetic Ovis aries embryos. However, at concentrations of 1 and 2% OF–UF, the blastocyst rate decreased in both groups of embryos. At a concentration of 1% OF–UF, both intracellular ROS and GSH decreased in the blastocysts of parthenogenetic embryos but not in cloned embryos. In cloned sheep embryos, OF–UF had no impact on the embryonic development or redox balance. Somatic cell nuclear transfer (SCNT) has great potential for the replication of high-commercial-value animals, threatened wild species for conservation purposes, and transgenic animals for biomedical purposes. However, SCNT has a low success rate due to intrinsic factors of the technique itself, which leads to low rates of embryonic development and epigenetic alterations in cloned embryos. The objective of this study was to evaluate the effect of OF–UF on the intracellular concentrations of ROS and GSH and the development of cloned and parthenogenetic Ovis aries embryos. The results do not show a beneficial effect on the development of parthenogenetic and cloned embryos at concentrations of 0.5% OF–UF. Furthermore, at 1% OF–UF, an adverse effect was observed in cloned embryos at the blastocyst stage and 2% OF and UF in parthenogenetic embryos during the first divisions. Decreases in ROS and GSH levels were observed in the parthenogenetic blastocysts treated with 1% OF–UF, but not in the clones, in which a higher concentration of GSH and a similar concentration of ROS were observed. No effect of OF–UF was observed on embryonic development and redox balance in sheep embryos cloned via handmade cloning. [ABSTRACT FROM AUTHOR]

Emotion dysregulation is a common challenge for autistic individuals, yet research examining early emotion regulation processes in autism is lacking. The present study examined negative emotion expression and parent-child co-regulatory processes in 18-month-old younger siblings of autistic children (children with an "elevated likelihood" (EL) of autism) with diverse outcomes: autism, language delay without autism (EL-LD), and no delay/diagnosis (EL-ND). Thirty-nine toddlers were videotaped at home with a parent. Negative emotion and parent co-regulatory responses were coded from the video. Results indicated that autistic toddlers exhibited more high-intensity negative emotion than EL-ND toddlers. The likelihood of negative emotion continuing once initiated was higher for autistic and EL-LD toddlers. Parental co-regulation strategy use was similar across groups. Parental co-regulation reduced the likelihood of continued negative emotion, although the effect appeared somewhat weaker for autistic toddlers. This research corroborates evidence that increased risk for heightened and prolonged negative emotion starts early in autistic children and, to a lesser extent, in EL-LD children. Parents of all children were highly responsive, but coregulatory responses may be less effective at reducing negative emotions for autistic children. While more research is needed, the present study contributes to our understanding of the unique emotional experiences of autistic toddlers. Managing negative emotion can be challenging for autistic individuals and their families from a young age. Parents help young children manage negative emotions by responding in comforting or supportive ways. Not much research has examined how negative emotions and parent responses to negative emotions are different in very young autistic children. This study used videotapes of 18-month-old toddlers and parents at home. We examined how much and how intensely toddlers expressed negative emotion in everyday situations, and how parents responded. Participants were younger siblings of autistic children, and we compared three groups—children that (1) later received an autism diagnosis; (2) had language delays but not autism; and (3) had no delays or autism. We found that autistic toddlers' negative emotion was more likely to be intense and to continue once it started compared with children without delays or autism. Language-delayed toddlers also showed some, but not all these differences. Parents responded similarly to negative emotions in all groups. When parents used strategies to help, it reduced the chances of the negative emotions continuing, although it may have been less helpful for autistic toddlers. This study shows that autistic children may express more intense and long-lasting negative emotions from an early age. It also shows that parents of autistic children are very responsive to their children's negative emotions, but these responses may not be as helpful to autistic children. While more research is needed, this study helps us understand how autistic toddlers may express and experience emotions differently. [ABSTRACT FROM AUTHOR]

Buses are among the most accessible and frequently used means of transport. Due to its importance, road safety analysis is frequently conducted to reduce accidents. This paper studied the relationship between weather conditions and the causes of accidents to improve road safety, focusing on long-distance services between Madrid and Bilbao (Spain). We employed Latent Class Clustering (LCC) and Hierarchical Ordered Logit models to identify these factors' relationships. Additionally, Kaplan-Meier survival analysis was adopted to provide temporal insights into accident occurrences. The main results show a downward trend in accidents since 2019, with manoeuvres being the most frequent cause. LCC reveals that "manoeuvres and car invading lanes in the opposite direction" in "clear and cloudy weather" has the highest probability of occurrence (63%). The hierarchical-ordered logit model indicates that rainy weather significantly affects all accident causes. Kaplan-Meier survival analysis reveals a vertical initial decline in survival probability within the first ten days, emphasizing a high initial accident risk. The integrated approach used in this work provides a thorough understanding of accident hazards, which is its main contribution. By integrating LCC, Hierarchical Ordered Logit models and Kaplan-Meier survival analysis; we could offer a comprehensive and nuanced interpretation of the connection between weather and bus accidents. The findings highlight the need for rapid and sustained safety interventions, enhancing robustness and providing actionable insights for improving bus safety. [ABSTRACT FROM AUTHOR]

This article examines the relationship between school climate and children's well-being, highlighting how factors such as coexistence, social relationships, and the physical and emotional environment influence their holistic development. The objective is to identify educational practices that promote a school environment conducive to children's well-being. The methodology used is a documentary review based on the PRISMA 2020 method, which enabled the selection of relevant articles through a rigorous process of identification, filtering, and evaluation. The main findings indicate that a positive school climate, characterized by teachers’ emotional support, inclusion, and the promotion of socio-emotional skills, significantly enhances students' emotional, social, and academic well-being. Socialization programs and peaceful conflict resolution were identified as key tools for fostering a safe and harmonious environment. In conclusion, school climate is crucial for children's well-being, and its improvement should be a priority in educational policies. Promoting healthy relationships and a safe environment not only enhances emotional development but also improves social adaptation and academic performance. [ABSTRACT FROM AUTHOR]

The objective of this article is to analyze the relationship between intrinsic and extrinsic motivation and academic performance in university students, using an integrated approach based on empirical studies and systematic reviews to identify key factors influencing academic success across various disciplines and educational contexts. The methodology employed is a literature review based on the PRISMA 2020 method, which ensured a rigorous selection of articles through inclusion and exclusion criteria, guaranteeing the quality and relevance of the studies. Included were studies addressing both intrinsic and extrinsic motivation and their impact on academic performance, excluding those lacking empirical results or focused on educational levels other than higher education. Results show that intrinsic motivation, characterized by personal interest and self-regulation, significantly influences academic performance, enhancing students' understanding and engagement. Meanwhile, extrinsic motivation, linked to external rewards and recognition, is particularly relevant in educational contexts like distance learning, where it helps sustain persistence and reduce dropout rates. Additionally, differences were observed in the effectiveness of motivational factors depending on the discipline and educational environment, such as sports-related careers and academic recovery programs. In conclusion, academic success relies on a balanced combination of intrinsic and extrinsic motivation. The findings highlight the importance of creating educational environments that foster both students' internal interest and external support, tailored to the specific needs of each academic context. [ABSTRACT FROM AUTHOR]

Karst morphology is a complex and highly systems and beneficial resource, it plays a fundamental role in geosystemic services and providing landscapes, water, energy and economic resources through tourism. Karst geodiversity is one of the main geomorphological components of the Napo Sumaco Aspiring UNESCO Global Geopark (NSAUGG), through which education and conservation strategies are directed locally. The present study focuses on deepening the knowledge of the morphologies of karst origin in the geopark, specifically in the municipality of Archidona (Ecuador), with the characterization of the types of structures at the micro (between 1 and 100 cm) and mesoscale (between 1 and 1000 m), in five sites located within the municipality. The abundance of microscale geoforms are related to a limited evolution of karst morphologies that can be observe in the local Amazonian karst system. Furthermore, an inverse relationship is established between the relative values of intrinsic geodiversity (IG) and karst disturbance (KDI) levels, being that the sites with lower levels of impact or disturbance show a greater diversity of karst morphologies. In this socio-environmental dynamic, the role played by the Napo Sumaco Aspiring UNESCO Global Geopark is fundamental, contributing to the strengthening of local capacities and the promotion of geoeducational and informative processes focused on the conservation of the karst region and its geodiversity in the municipality of Archidona. [ABSTRACT FROM AUTHOR]

The instruction of addition and subtraction during the third year of Basic General Education fosters students' autonomy and confidence in their ability to address mathematical problems. By facing and overcoming challenges, children cultivate a positive attitude towards mathematics and become more confident when facing numerical problems in various situations. The objective of this research is to develop a methodological strategy for solving addition and subtraction problems in the third year of Basic General Education. The research was carried out in the Ciudad de Ibarra Educational Unit. The sample consisted of 28 students of said educational level. Qualitative and quantitative methods were used with the objective of measuring the impact of the proposed strategy and the development of skills in addition and subtraction. From the implementation of the proposed strategy, a significant improvement was obtained in the development for solving addition and subtraction problems in the third year of the EGB. [ABSTRACT FROM AUTHOR]

Introduction: Cannabis ranks as one of the most widely used psychoactive substances worldwide. Its usage has been reported as a risk factor for developing a variety of physical and mental health issues, alongside social and economic problems. According to the World Health Organization (WHO), hazardous (or high-risk) substance use is defined as "a pattern of substance use that increases the risk of harmful consequences for the user." The primary objective of this study was to achieve a consensus on an operational definition of high-risk cannabis use suitable for a European jurisdiction (Spain). Methods: A three round Delphi study was conducted. The Delphi technique entails group decision-making and iterative forecasting by consulting a panel of experts and conducting systematic feedback rounds. An online survey was distributed among a diverse expert panel comprising professionals from Spain working on fields related to cannabis use (research, policy planning, and clinical professionals). One-hundred ninety-nine invitations were sent to experts, of which forty-five (22.6%) accepted to participate. All participants were provided the option to revise a background document with the latest compiled scientific evidence and guidelines related to health and social conditions associated to cannabis usage. This background document also proposed the quantification of dose using a standardized unit established for the population of Spain (the Standard Joint Unit or SJU) based on quantity of cannabis main psychoactive constituent, 9-tetrahydrocannabinol (9-THC) (1 SJU = 1 joint = 0.25 g of cannabis = 7 mg of 9-THC). Three rounds of Likert scale and open-ended questions were administered until consensus, defined as ≥75% agreement, was attained. Results: Consensus was reached on defining high-risk cannabis use as the usage of more than 4 SJU (>28 mg THC) per week or any use of cannabis with potency >10% THC. Concurrent use of cannabis with other drugs was also considered high risk, while the smoked route was considered the riskiest administration route. It was also agreed that vulnerable groups for high-risk cannabis use (for whom any pattern of cannabis use represents high risk of harm) include individuals under the age of 21, pregnant or breastfeeding women, individuals with psychiatric history, those with medical health issues related to cannabis use, professional drivers and heavy machine operators. Conclusion: This operational definition of high-risk cannabis use for Spain elucidates usage patterns and individual vulnerability factors predictive of heightened harm. Its adoption holds potential to inform decision-making among individuals, professionals, and policy-makers, facilitating evidence-based interventions aimed at prevention and risk reduction. [ABSTRACT FROM AUTHOR]

Secondary envelopment of the human cytomegalovirus (HCMV) is a critical but not well-understood process that takes place at the cytoplasmic viral assembly complex (cVAC) where nucleocapsids acquire their envelope by budding into cellular membranes containing viral glycoproteins. Previous studies presented controversial results regarding the composition of the viral envelope, suggesting trans-Golgi and endosomal origins, as well as intersections with the exosomal and endocytic pathways. Here, we investigated the role of endocytic membranes for the secondary envelopment of HCMV by using wheat germ agglutinin (WGA) pulse labeling to label glycoproteins at the plasma membrane and to follow their trafficking during HCMV infection by light microscopy and transmission electron microscopy (TEM). WGA labeled different membrane compartments within the cVAC, including early endosomes, multivesicular bodies, trans-Golgi, and recycling endosomes. Furthermore, TEM analysis showed that almost 90% of capsids undergoing secondary envelopment and 50% of enveloped capsids were WGA-positive within 90 min. Our data reveal extensive remodeling of the endocytic compartment in the late stage of HCMV infection, where the endocytic compartment provides an optimized environment for virion morphogenesis and serves as the primary membrane source for secondary envelopment. Furthermore, we show that secondary envelopment is a rapid process in which endocytosed membranes are transported from the plasma membrane to the cVAC within minutes to be utilized by capsids for envelopment. [ABSTRACT FROM AUTHOR]

Understanding the combined effects of environmental heterogeneity and evolutionary processes on marine populations is a primary goal of seascape genomic approaches. Here, we utilized genomic approaches to identify local adaptation signatures in Littoraria flava , a widely distributed marine gastropod in the tropical West Atlantic population. We also performed molecular evolution analyses to investigate potential selective signals across the genome. After obtaining 6,298 and 16,137 single nucleotide polymorphisms derived from genotyping-by-sequencing and RNA sequencing, respectively, 69 from genotyping-by-sequencing (85 specimens) and four from RNA sequencing (40 specimens) candidate single nucleotide polymorphisms were selected and further evaluated. The correlation analyses support different evolutionary pressures over transcribed and non-transcribed regions. Thus, single nucleotide polymorphisms within transcribed regions could account for the genotypic and possibly phenotypic divergences in periwinkles. Our molecular evolution tests based on synonymous and non-synonymous ratio (kN/kS) showed that genotype divergences containing putative adaptive single nucleotide polymorphisms arose mainly from synonymous and/or UTR substitutions rather than polymorphic proteins. The distribution of genotypes across different localities seems to be influenced by marine currents, pH, and temperature variations, suggesting that these factors may impact the species dispersion. The combination of RNA sequencing and genotyping-by-sequencing derived datasets provides a deeper understanding of the molecular mechanisms underlying selective forces responses on distinct genomic regions and could guide further investigations on seascape genomics for non-model species. [ABSTRACT FROM AUTHOR]

This article, published in Aquatic Mammals, discusses the tracking of a white humpback whale in the eastern South Pacific. The study focuses on the first-year survival of a white humpback whale, which is a rare occurrence in nature. The researchers document and analyze sightings of the whale in Costa Rica, Peru, and Antarctica, providing valuable insights into the migratory movements of the species in the region. The document also discusses the rarity of hypopigmented cetaceans and the challenges they face due to their coloration. The authors encourage long-term observation of the whale and other cetaceans using citizen science and social media. Additionally, there is a list of references related to humpback whales, covering topics such as their breeding cycle, migration patterns, entanglement in fishing gear, and sightings of white or leucistic individuals. These references provide valuable information for researchers studying humpback whales and their conservation, as well as for individuals interested in learning more about these majestic creatures. [Extracted from the article]

Myocardial reperfusion injury (MRI) accounts for up to 50% of the final size in acute myocardial infarction and other conditions associated with ischemia‐reperfusion. Currently, there is still no therapy to prevent MRI, but it is well known that oxidative stress has a key role in its mechanism. We previously reduced MRI in rats through a combined antioxidant therapy (CAT) of ascorbic acid, N‐acetylcysteine, and deferoxamine. This study determines the safety and pharmacokinetics of CAT in a Phase I clinical trial. Healthy subjects (n = 18) were randomized 2:1 to CAT or placebo (NaCl 0.9% i.v.). Two different doses/infusion rates of CATs were tested in a single 90‐minute intravenous infusion. Blood samples were collected at specific times for 180 minutes to measure plasma drug concentrations (ascorbic acid, N‐acetylcysteine, and deferoxamine) and oxidative stress biomarkers. Adverse events were registered during infusion and followed for 30 days. Both CAT1 and CAT2 significantly increased the CAT drug concentrations compared to placebo (P <.05). Most of the pharmacokinetic parameters were similar between CAT1 and CAT2. In total, 6 adverse events were reported, all nonserious and observed in CAT1. The ferric‐reducing ability of plasma (an antioxidant biomarker) increased in both CAT groups compared to placebo (P <.001). The CAT is safe in humans and a potential treatment for patients with acute myocardial infarction undergoing reperfusion therapy. [ABSTRACT FROM AUTHOR]

This article presents a study on how digital mediation in the teaching-learning process of Modern and Contemporary Physics concepts in elementary school establishes the production of hybrid mechanisms of cognition between humans and non-humans, in light of Bruno Latour's generalized symmetry. To this end, we chose as knowledge content the phenomenon of nuclear fusion, foreseen as an object of knowledge in the National Common Curricular Base (BNCC) of Natural Sciences. In view of this, we adopted the articulation of three theoretical references, the Network Cognitive Mediation Theory (TMC), responsible for discussing the mediation mechanisms as processing external to the human brain and its implications on the cognitive structure of students, the Theory of Mental Models that classifies and categorizes the types of mental representations and Bruno Latour's Symmetrical Anthropology that provides a systemic, reticular and nondichotomized view of the construction of reality. The initial results were obtained after analyzing the questionnaires and the students' verbal and sign languages. Subsequently, we interpreted the results in accordance with the concepts of hybridization, detours and translations of Latourian thought. We found that digital mediation in this context influences the cognitive mechanisms used by students and the sophistication of the mental representations constructed by them. Finally, we recognize that both the teaching-learning process and the construction of mental representations based on digital mediation constitute Latourian hybrids, as the actors involved begin to share human and non-human skills. [ABSTRACT FROM AUTHOR]

Common cutworm, Spodoptera litura is an important pest of corn causing significant crop yield loss. Synthetic insecticides have mostly been used to combat this pest, raising human and environmental health concerns. Plant growth-promoting rhizobacteria (PGPR) could compensate for or augment the harmful effects of agrochemicals. Herein, we aimed to assess whether PGPR-induced defenses in corn plants impact the host-plant selection behavior of S. litura. Headspace volatile organic compounds were analyzed using gas chromatography–mass spectrometry. Larvae fed inoculated corn exhibited lower weights and relative growth rate than noninoculated plants. Under choice experiments, PGPR-treated plants significantly reduced percentage leaf damage area and oviposition rate compared to untreated plants. Volatile organic compound ratio emission varied significantly between control and PGPR treatments, which, in part, explains feeding and oviposition deterrence in PGPR-treated plants. The results demonstrate that PGPR inoculation can enhance corn resistance to S. litura , making it a promising candidate for crop protection strategies. [ABSTRACT FROM AUTHOR]

Target 3 in the Kunming–Montreal Global Biodiversity Framework (GBF) calls for protecting at least 30% of the world's lands and waters in area‐based conservation approaches by 2030. This ambitious 30×30 target has spurred great interest among policy makers, practitioners, and researchers in defining and measuring the effectiveness of these types of approaches. But along with this broad interest, there has also been a proliferation of terms and their accompanying abbreviations used to describe different types of conservation areas and their governance, planning, management, and monitoring. The lack of standard terms is hindering the use and assessment of area‐based approaches to conserve the world's biodiversity. It is difficult to track progress toward GBF Target 3 or to share learning with other practitioners if different groups of people are using different words to describe the same concept or similar words to talk about different concepts. To address this problem, the International Union for Conservation of Nature's World Commission on Protected Areas commissioned a task force to review existing terms and recommend a standard English‐language lexicon for this field based on key criteria. The results were definitions of 37 terms across 6 categories, including types of protected and additional conservation areas (e.g., protected area, additional conservation area), sets of these areas (protected area network, protected area system), their governance and management (governance, rightsholders), assessment (effectiveness, equitability), spatial planning (key biodiversity area), and action planning (value, outcome, objective). Our standard lexicon can provide a common language for people who want to use it and a shared reference point that can be used to translate various terms used by different groups. The common understanding provided by the lexicon can serve as a foundation for collaborative efforts to improve the policies, implementation, assessments, research, and learning about this important set of conservation approaches. [ABSTRACT FROM AUTHOR]

Purpose: Gastric cancer (GC) is among the leading causes of cancer mortality worldwide. The objective of this study was to investigate the association between dietary fiber intake and GC. Methods: We pooled data from 11 population or hospital-based case–control studies included in the Stomach Cancer Pooling (StoP) Project, for a total of 4865 histologically confirmed cases and 10,626 controls. Intake of dietary fibers and other dietary factors was collected using food frequency questionnaires. We calculated the odds ratios (OR) and 95% confidence intervals (CI) of the association between dietary fiber intake and GC by using a multivariable logistic regression model adjusted for study site, sex, age, caloric intake, smoking, fruit and vegetable intake, and socioeconomic status. We conducted stratified analyses by these factors, as well as GC anatomical site and histological type. Results: The OR of GC for an increase of one quartile of fiber intake was 0.91 (95% CI: 0.85, 0.97), that for the highest compared to the lowest quartile of dietary fiber intake was 0.72 (95% CI: 0.59, 0.88). Results were similar irrespective of anatomical site and histological type. Conclusion: Our analysis supports the hypothesis that dietary fiber intake may exert a protective effect on GC. [ABSTRACT FROM AUTHOR]

Nonlinear distinct models have wide applications in various fields of science and engineering. The present research uses the mapping and generalized Riccati equation mapping methods to address the exact solutions for the nonlinear Klein–Gordon equation. First, the travelling wave transform is used to create an ordinary differential equation form for the nonlinear partial differential equation. This work presents the construction of novel trigonometric, hyperbolic and Jacobi elliptic functions to the nonlinear Klein–Gordon equation using the mapping and generalized Riccati equation mapping methods. In the fields of fluid motion, plasma science, and classical physics the nonlinear Klein–Gordon equation is frequently used to identify of a wide range of interesting physical occurrences. It is considered that the obtained results have not been established in prior study via these methods. To fully evaluate the wave character of the solutions, a number of typical wave profiles are presented, including bell-shaped wave, anti-bell shaped wave, W-shaped wave, continuous periodic wave, while kink wave, smooth kink wave, anti-peakon wave, V-shaped wave and flat wave solitons. Several 2D, 3D and contour plots are produced by taking precise values of parameters in order to improve the physical description of solutions. It is noteworthy that the suggested techniques for solving nonlinear partial differential equations are capable, reliable, and captivating analytical instruments. [ABSTRACT FROM AUTHOR]

Two-dimensional (2D) materials have emerged as a frontier in materials science, offering unique properties due to their atomically thin nature. Among these materials, bismuthene stands out due to its exceptional optical, electronic, and catalytic characteristics. Bismuthene exhibits high charge carrier mobility, stability, and a tunable bandgap (0.3–1.0 eV), making it highly suitable for applications in transistors, spintronics, biomedicine, and photocatalysis. This work explores the so far reported synthesis methods for obtaining 2D bismuthene, including bottom-up approaches like chemical vapor deposition and molecular beam epitaxy, and top-down methods such as liquid-phase exfoliation and mechanical exfoliation. Recent advancements in understanding 2D bismuthene structural phases, electronic properties modulated by spin-orbit coupling, and its potential applications in next-generation photocatalysts are also reviewed. As is retrieved by our literature review, 2D bismuthene shows great promise for addressing significant environmental challenges. For instance, in CO2 reduction, integrating bismuthene into 2D/2D heterostructures enhances electron transfer efficiency, thereby improving selectivity toward valuable products, such as CH4 and formic acid. In organic pollutant degradation, bismuth subcarbonate (Bi2O2CO3) nanosheets, obtained from 2D bismuthene, have demonstrated high photocatalytic degradation of antibiotics under visible light irradiation, due to their increased surface area and efficient generation of reactive species. Moreover, bismuthene-based materials exhibit potential in the photocatalytic water-splitting process for hydrogen production, overcoming issues associated with UV-light dependence and sacrificial agent usage. This review underscores the versatile applications of 2D bismuthene in advancing photocatalytic technologies, offering insights into future research directions and potential industrial applications. [ABSTRACT FROM AUTHOR]

ABSTRACT Introduction Methods Case Conclusion Congenital complete heart block is a condition where there is a risk of Stokes Adam's attacks and sudden death may occur. Once the escape rate is too low, or other high‐risk factors occur, these patients ultimately need pacemakers placed. Epicardial or transvenous pacemakers have typically been in employed dependent on size of the patient and other circumstances. We describe the first case of an implant via internal jugular vein (right) of a dual chamber leadless pacemaker implant in a symptomatic pediatric patient with congenital complete heart block.The study was approved by the University of California and consent was waived due to retrospective nature of this case report.A 13‐year‐old presented with presyncope at rest after years of being followed for her congenital complete heart block. Her average rate on Holter monitoring was below 50 bpm, which coincided with her recent symptoms. After discussion with family, and our own cardiology/surgical team, she had a dual chamber leadless pacemaker implanted. Stable 3‐month atrial parameters included an impedance of 340 Ω, sensing of 3.2 mV, and threshold of 0.25 V at 0.2 ms, while ventricular parameters showed an impedance of 780 Ω, sensing of 14.2  mV, and threshold of 0.5 V at 0.2 ms.Dual chamber leadless pacemaker implant is feasible via right internal jugular vein access and in a pediatric patient. [ABSTRACT FROM AUTHOR]

The article discusses the impact of dermoscopy training, including artificial intelligence, on general practitioner residents in France. The study aimed to assess diagnostic accuracy, lesion nature determination, and therapeutic strategy proposal. Results showed significant improvement in diagnostic accuracy after training sessions, with AI leading to even better outcomes. The study suggests that dermoscopy training, especially with AI integration, could enhance early diagnosis in clinical practice, prompting consideration for inclusion in official university systems. [Extracted from the article]

Squamous cell carcinoma (SCC) is a malignant epithelial neoplasm originating from keratinocytes. It affects areas that have little or no fur, with the oral cavity being the fourth most affected area. Despite its low metastatic capacity, it can affect regional lymph nodes and therefore lungs and bones. The objective of this study is to report the case of a feline with SCC in the labial region that underwent surgery to remove the nodule and mandibular lymph nodes for histopathological analysis. The assessment of metastatic involvement of the bilateral mandibular lymph node allowed the start of systemic chemotherapy, promoting the oncologic staging of the patient. [ABSTRACT FROM AUTHOR]

Urolithiasis is a common urinary tract condition frequently encountered in veterinary practice. This report aims to present the case of a 4-year-old female German Spitz dog with a historic of recurrent struvite urinary calculi in the bladder. The treatment of choice is cystotomy surgery for complete removal of the urinary calculi. Therefore, the combination of cystotomy and urine culture is important for clinical and surgical management in order to achieve more favorable outcomes and prognoses. [ABSTRACT FROM AUTHOR]

The internal atmospheric waves are gravity waves and occur in the inner part of the fluid system. In this study, a time-fractional model for internal atmospheric waves is investigated with the Caputo-Fabrizio time-fractional differential operator. The analytical solution of the considered model is retrieved by the Elzaki Adomian decomposition method. The variation in the solution is examined for increasing order of the fractional parameter α through numerical and graphical simulations. The accuracy of the obtained results is established by comparing the obtained solution of considered fractional model with the results available in the literature. [ABSTRACT FROM AUTHOR]

Objective: Insertion and deletion (indel) analysis of CRISPR-Cas guide RNAs (gRNAs) is crucial in gene editing to assess gRNA efficiency and indel frequency. This study evaluates the utility of CRISPResso2 with Oxford Nanopore sequencing data (nCRISPResso2) for gRNA indel screening, compared to two common Sanger sequencing-based methods, TIDE and ICE. To achieve this, sheep and horse fibroblasts were transfected with Cas9 and a gRNA targeting the myostatin (MSTN) gene. DNA was subsequently extracted, and PCR products exceeding 600 bp were sequenced using both Sanger and Nanopore sequencing. Indel profiling was then conducted using TIDE, ICE, and nCRISPResso2. Results: Comparison revealed close correspondence in indel formation among methods. For the sheep MSTN gRNA, indel percentages were 52%, 58%, and 64% for TIDE, ICE, and nCRISPResso2, respectively. Horse MSTN gRNA showed 81%, 87%, and 86% edited amplicons for TIDE, ICE, and nCRISPResso2. The frequency of each type of indel was also comparable among the three methods, with nCRISPResso2 and ICE aligning the closest. nCRISPResso2 offers a viable alternative for CRISPR-Cas gRNA indel screening, especially with large amplicons unsuitable for Illumina sequencing. CRISPResso2's compatibility with Nanopore data enables cost-effective and efficient indel profiling, yielding results comparable to common Sanger sequencing-based methods. [ABSTRACT FROM AUTHOR]

Caffeic acid (CA) is a polyphenol belonging to the phenylpropanoid family, commonly found in plants and vegetables. It was first identified by Hlasiwetz in 1867 as a breakdown product of caffetannic acid. CA is biosynthesized from the amino acids tyrosine or phenylalanine through specific enzyme-catalyzed reactions. Extensive research since its discovery has revealed various health benefits associated with CA, including its antioxidant, anti-inflammatory, and anticancer properties. These effects are attributed to its ability to modulate several pathways, such as inhibiting NFkB, STAT3, and ERK1/2, thereby reducing inflammatory responses, and activating the Nrf2/ARE pathway to enhance antioxidant cell defenses. The consumption of CA has been linked to a reduced risk of certain cancers, mitigation of chemotherapy and radiotherapy-induced toxicity, and reversal of resistance to first-line chemotherapeutic agents. This suggests that CA could serve as a useful adjunct in cancer treatment. Studies have shown CA to be generally safe, with few adverse effects (such as back pain and headaches) reported. This review collates the latest information from Google Scholar, PubMed, the Phenol-Explorer database, and ClinicalTrials.gov, incorporating a total of 154 articles, to underscore the potential of CA in cancer prevention and overcoming chemoresistance. [ABSTRACT FROM AUTHOR]

In the Tulcán Canton, Carchi Province, 22 cases of hearing impairment were identified in the educational sector. According to the National Council for Disability Equality (CODANIS 2023), the lack of adequate institutions and the limited training of teachers in sign language and inclusive methodologies hinder the learning of these students. At the José María Velasco Ibarra Basic General Education School (EGB), serious difficulties in the educational inclusion process are observed, the objective of the research is to design an institutional guide to improve the school inclusion of students with hearing disabilities. A theoretical approach encompassing educational inclusion and the characterization of hearing disabilities was utilized, applying a mixed methodological design that includes both quantitative and qualitative data collection techniques, a survey was conducted with 27 teachers, and interviews were held with parents and administrators, revealing that educators lack knowledge about hearing disabilities, although they show a positive attitude towards inclusion. The results indicate that, despite shortcomings in inclusion, a guide was successfully designed in three modules: theoretical aspects of hearing disabilities, teaching actions, and sign language, complemented by the use of TIC. [ABSTRACT FROM AUTHOR]