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This study was performed to evaluate the response of different newly established tomato (Lycopersicon esculentum Mill.) cultivars (Búfalo [control, a widely cultivated variety], Corindon, Dombelo, GC-773, GC-775, Nancy, Noa, Sarky, Yunque, Volcani, 617/83, and 2084/81), all cultivated under identical greenhouse conditions, in relation to the efficiency in macronutrient utilization to ascertain whether the genotype and the plant developmental stages affect the concentrations of nitrogen (N), phosphorus (P), potassium (K), sodium (Na), calcium (Ca), magnesium (Mg), and sulfur (S) in leaves. The cultivar with greater foliar-biomass production (Volcani) or greater concentration of nutrients as organic N (Noa, Yunque, 617/83) showed less marketable and more non-commercial fruit production. The phenological phases of developing and ripening fruits significantly increased the concentration of P and reduced the concentration of all N forms and total K, as well as Na, Ca, Mg, and S forms in the leaves. [ABSTRACT FROM AUTHOR]