11 results on '"Allert, Stefanie"'
Search Results
2. Candida albicans translocation through the intestinal epithelial barrier is promoted by fungal zinc acquisition and limited by NFκB-mediated barrier protection.
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Sprague, Jakob L., Schille, Tim B., Allert, Stefanie, Trümper, Verena, Lier, Adrian, Großmann, Peter, Priest, Emily L., Tsavou, Antzela, Panagiotou, Gianni, Naglik, Julian R., Wilson, Duncan, Schäuble, Sascha, Kasper, Lydia, and Hube, Bernhard
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CANDIDA albicans , *INTESTINES , *ZINC , *INTESTINAL infections , *TEMPOROPARIETAL junction , *EPITHELIAL cells , *PEPTIDES - Abstract
The opportunistic fungal pathogen Candida albicans thrives on human mucosal surfaces as a harmless commensal, but frequently causes infections under certain predisposing conditions. Translocation across the intestinal barrier into the bloodstream by intestine-colonizing C. albicans cells serves as the main source of disseminated candidiasis. However, the host and microbial mechanisms behind this process remain unclear. In this study we identified fungal and host factors specifically involved in infection of intestinal epithelial cells (IECs) using dual-RNA sequencing. Our data suggest that host-cell damage mediated by the peptide toxin candidalysin-encoding gene ECE1 facilitates fungal zinc acquisition. This in turn is crucial for the full virulence potential of C. albicans during infection. IECs in turn exhibit a filamentation- and damage-specific response to C. albicans infection, including NFκB, MAPK, and TNF signaling. NFκB activation by IECs limits candidalysin-mediated host-cell damage and mediates maintenance of the intestinal barrier and cell-cell junctions to further restrict fungal translocation. This is the first study to show that candidalysin-mediated damage is necessary for C. albicans nutrient acquisition during infection and to explain how IECs counteract damage and limit fungal translocation via NFκB-mediated maintenance of the intestinal barrier. Author summary: Candida albicans populations colonizing the intestine serve as the main source for systemic infections. Though normally commensal, under certain conditions, C. albicans can translocate across the intestine and into the bloodstream, leading to systemic candidiasis. Here we dissect the fungal and host activities involved in this process. We find that damage to host cells, which supports efficient translocation, is associated with active acquisition of host-cell zinc by C. albicans. At the same time, intestinal epithelial cells foster barrier integrity to limit fungal translocation independently of host damage. [ABSTRACT FROM AUTHOR]
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- 2024
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3. In Vivo Transcriptional Profiling of Human Pathogenic Fungi during Infection: Reflecting the Real Life?
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Allert, Stefanie, Brunke, Sascha, and Hube, Bernhard
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GENE expression profiling , *PATHOGENIC fungi , *INFECTION , *GENE expression , *RNA sequencing - Abstract
The article focuses on the in vivo transcriptional profiling of human pathogenic fungi during infection. Topics include the microbial infections in humans caused by viruses, bacteria, fungi, or parasites, the identification of infections through genomes of the pathogen and host and appropriate gene expression, and the RNA sequencing.
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- 2016
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4. Fungal biotin homeostasis is essential for immune evasion after macrophage phagocytosis and virulence.
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Sprenger, Marcel, Hartung, Teresa S., Allert, Stefanie, Wisgott, Stephanie, Niemiec, Maria J., Graf, Katja, Jacobsen, Ilse D., Kasper, Lydia, and Hube, Bernhard
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BIOTIN , *PHAGOCYTOSIS , *CANDIDA albicans , *HOMEOSTASIS , *TRANSCRIPTION factors , *FUNGUS-bacterium relationships - Abstract
Biotin is an important cofactor for multiple enzymes in central metabolic processes. While many bacteria and most fungi are able to synthesise biotin de novo, Candida spp. are auxotrophic for this vitamin and thus require efficient uptake systems to facilitate biotin acquisition during infection. Here we show that Candida glabrata and Candida albicans use a largely conserved system for biotin uptake and regulation, consisting of the high‐affinity biotin transporter Vht1 and the transcription factor Vhr1. Both species induce expression of biotin‐metabolic genes upon in vitro biotin depletion and following phagocytosis by macrophages, indicating low biotin levels in the Candida‐containing phagosome. In line with this, we observed reduced intracellular proliferation of both Candida cells pre‐starved of biotin and deletion mutants lacking VHR1 or VHT1 genes. VHT1 was essential for the full virulence of C. albicans during systemic mouse infections, and the lack of VHT1 led to reduced fungal burden in C. glabrata‐infected brains and C. albicans‐infected brains and kidneys. Together, our data suggest a critical role of Vht1‐mediated biotin acquisition for C. glabrata and C. albicans during intracellular growth in macrophages and systemic infections. [ABSTRACT FROM AUTHOR]
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- 2020
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5. FSP1-specific <italic>SMAD2</italic> knockout in renal tubular, endothelial, and interstitial cells reduces fibrosis and epithelial-to-mesenchymal transition in murine STZ-induced diabetic nephropathy.
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Loeffler, Ivonne, Liebisch, Marita, Allert, Stefanie, Kunisch, Elke, Kinne, Raimund W., and Wolf, Gunter
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FIBROSIS , *RENAL tubular transport , *EPITHELIAL cells , *DIABETIC nephropathies , *TRANSFORMING growth factors - Abstract
Extracellular matrix deposition during tubulointerstitial fibrosis (TIF), a central pathological process in patients with diabetic nephropathy (DN), is driven by locally activated, disease-relevant myofibroblasts. Myofibroblasts can arise from various cellular sources, e.g., tubular epithelial cells via a process named epithelial-to-mesenchymal transition (EMT). Transforming growth factor beta 1 (TGF-β1) and its downstream Smad signaling play a critical role in both TIF and EMT. Whereas Smad3 is one central mediator, the role of the other prominently expressed variant, Smad2, is not completely understood. In this study, we sought to analyze the role of renal Smad2 in the development of TIF and EMT during streptozotocin-induced DN by using a fibroblast-specific protein 1 (FSP1)-promotor-driven
SMAD2 knockout mouse model with decreased tubular, endothelial, and interstitial Smad2 expression. In contrast to wild-type diabetic mice, diabeticSMAD2 knockout mice showed the following features: (1) significantly reduced DN and TIF (shown by KIM1 expression; periodic acid Schiff staining; collagen I and III, fibronectin, and connective tissue growth factor deposition); (2) significantly reduced tubular EMT-like changes (e.g., altered Snail1, E-cadherin, matrix metalloproteinase 2, and vimentin deposition); and (3) significantly decreased expression of myofibroblast markers (α-smooth muscle actin, FSP1). As one mechanism for the protection against diabetes-induced TIF and EMT, decreased Smad3 protein levels and, as a possible consequence, reduced TGF-β1 levels were observed in diabeticSMAD2 knockout mice. Our findings thus support the important role of Smad2 for pro-fibrotic TGF-β/Smad3 signaling in experimental DN. [ABSTRACT FROM AUTHOR]- Published
- 2018
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6. Candidalysin Is the Hemolytic Factor of Candida albicans.
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Mogavero, Selene, Höfs, Sarah, Lauer, Alexa N., Müller, Rita, Brunke, Sascha, Allert, Stefanie, Gerwien, Franziska, Groth, Sabrina, Dolk, Edward, Wilson, Duncan, Gutsmann, Thomas, and Hube, Bernhard
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CANDIDA albicans , *PEPTIDES , *PURINERGIC receptors , *IMMUNOGLOBULINS , *ARTIFICIAL membranes , *HEMOLYSIS & hemolysins - Abstract
Candida albicans produces an important virulence factor, the hypha-associated Ece1-derived secreted peptide toxin candidalysin, which is crucial for the establishment of mucosal and systemic infections. C. albicans has also long been known to be hemolytic, yet the hemolytic factor has not been clearly identified. Here, we show that candidalysin is the hemolytic factor of C. albicans. Its hemolytic activity is modulated by fragments of another Ece1 peptide, P7. Hemolysis by candidalysin can be neutralized by the purinergic receptor antagonist pyridoxal-phosphate-6-azophenyl-2′,4′-disulfonic acid (PPADS). PPADS also affects candidalysin's ability to intercalate into synthetic membranes. We also describe the neutralization potential of two anti-candidalysin nanobodies, which are promising candidates for future anti-Candida therapy. This work provides evidence that the historically proposed hemolytic factor of C. albicans is in fact candidalysin and sheds more light on the complex roles of this toxin in C. albicans biology and pathogenicity. [ABSTRACT FROM AUTHOR]
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- 2022
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7. Candidalysin delivery to the invasion pocket is critical for host epithelial damage induced by Candida albicans.
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Mogavero, Selene, Sauer, Frank M., Brunke, Sascha, Allert, Stefanie, Schulz, Daniela, Wisgott, Stephanie, Jablonowski, Nadja, Elshafee, Osama, Krüger, Thomas, Kniemeyer, Olaf, Brakhage, Axel A., Naglik, Julian R., Dolk, Edward, and Hube, Bernhard
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CANDIDA albicans , *IMMUNOGLOBULINS , *PATHOGENIC fungi , *EPITHELIAL cells , *CELL membranes , *SECRETION - Abstract
The human pathogenic fungus Candida albicans is a frequent cause of mucosal infections. Although the ability to transition from the yeast to the hypha morphology is essential for virulence, hypha formation and host cell invasion per se are not sufficient for the induction of epithelial damage. Rather, the hypha‐associated peptide toxin, candidalysin, a product of the Ece1 polyprotein, is the critical damaging factor. While synthetic, exogenously added candidalysin is sufficient to damage epithelial cells, the level of damage does not reach the same level as invading C. albicans hyphae. Therefore, we hypothesized that a combination of fungal attributes is required to deliver candidalysin to the invasion pocket to enable the full damaging potential of C. albicans during infection. Utilising a panel of C. albicans mutants with known virulence defects, we demonstrate that the full damage potential of C. albicans requires the coordinated delivery of candidalysin to the invasion pocket. This process requires appropriate epithelial adhesion, hyphal extension and invasion, high levels of ECE1 transcription, proper Ece1 processing and secretion of candidalysin. To confirm candidalysin delivery, we generated camelid VHHs (nanobodies) specific for candidalysin and demonstrate localization and accumulation of the toxin only in C. albicans‐induced invasion pockets. In summary, a defined combination of virulence attributes and cellular processes is critical for delivering candidalysin to the invasion pocket to enable the full damage potential of C. albicans during mucosal infection. Take Aways: Candidalysin is a peptide toxin secreted by C. albicans causing epithelial damage.Candidalysin delivery to host cell membranes requires specific fungal attributes.Candidalysin accumulates in invasion pockets created by invasive hyphae.Camelid nanobodies enabled visualisation of candidalysin in the invasion pocket. [ABSTRACT FROM AUTHOR]
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- 2021
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8. A variant ECE1 allele contributes to reduced pathogenicity of Candida albicans during vulvovaginal candidiasis.
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Liu, Junyan, Willems, Hubertine M. E., Sansevere, Emily A., Allert, Stefanie, Barker, Katherine S., Lowes, David J., Dixson, Andrew C., Xu, Zhenbo, Miao, Jian, DeJarnette, Christian, Tournu, Helene, Palmer, Glen E., Richardson, Jonathan P., Barrera, Francisco N., Hube, Bernhard, Naglik, Julian R., and Peters, Brian M.
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VULVOVAGINAL candidiasis , *CANDIDA albicans , *PATHOLOGY , *GENETIC variation , *ALLELES - Abstract
Vulvovaginal candidiasis (VVC), caused primarily by the human fungal pathogen Candida albicans, results in significant quality-of-life issues for women worldwide. Candidalysin, a toxin derived from a polypeptide (Ece1p) encoded by the ECE1 gene, plays a crucial role in driving immunopathology at the vaginal mucosa. This study aimed to determine if expression and/or processing of Ece1p differs across C. albicans isolates and whether this partly underlies differential pathogenicity observed clinically. Using a targeted sequencing approach, we determined that isolate 529L harbors a similarly expressed, yet distinct Ece1p isoform variant that encodes for a predicted functional candidalysin; this isoform was conserved amongst a collection of clinical isolates. Expression of the ECE1 open reading frame (ORF) from 529L in an SC5314-derived ece1Δ/Δ strain resulted in significantly reduced vaginopathogenicity as compared to an isogenic control expressing a wild-type (WT) ECE1 allele. However, in vitro challenge of vaginal epithelial cells with synthetic candidalysin demonstrated similar toxigenic activity amongst SC5314 and 529L isoforms. Creation of an isogenic panel of chimeric strains harboring swapped Ece1p peptides or HiBiT tags revealed reduced secretion with the ORF from 529L that was associated with reduced virulence. A genetic survey of 78 clinical isolates demonstrated a conserved pattern between Ece1p P2 and P3 sequences, suggesting that substrate specificity around Kex2p-mediated KR cleavage sites involved in protein processing may contribute to differential pathogenicity amongst clinical isolates. Therefore, we present a new mechanism for attenuation of C. albicans virulence at the ECE1 locus. Author summary: Vulvovaginal candidiasis (VVC), caused primarily by the human fungal pathogen Candida albicans, results in significant quality-of-life issues for women worldwide. Candidalysin, a toxin derived from a polypeptide (Ece1p) encoded by the ECE1 gene, plays a crucial role in driving disease pathology. In this study, we aimed to determine if genetic variation of ECE1 existed across a collection of C. albicans clinical isolates. Using a targeted sequencing approach, we identified a conserved variant candidalysin isoform. We then constructed isogenic chimeric strains harboring swapped Ece1p peptides or HiBiT tags between representative isoforms and showed impaired secretion with the variant that was associated with reduced virulence in vitro and in vivo. We also elucidated a conserved pattern between key sequences near lysine-arginine cleavage sites involved in Ece1p polypeptide processing by the Kex2p enzyme that correlate with predicted and experimental candidalysin secretion. Therefore, we present a novel mechanism for attenuation of C. albicans virulence at the ECE1 locus that may partly underlie differential clinical outcomes and contribute to the balance between commensal and pathogen at the vaginal mucosa. [ABSTRACT FROM AUTHOR]
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- 2021
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9. The Snf1-activating kinase Sak1 is a key regulator of metabolic adaptation and in vivo fitness of Candida albicans.
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Ramírez‐Zavala, Bernardo, Mottola, Austin, Haubenreißer, Julia, Schneider, Sabrina, Allert, Stefanie, Brunke, Sascha, Ohlsen, Knut, Hube, Bernhard, and Morschhäuser, Joachim
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CANDIDA albicans , *BACTERIAL genetics , *PHENOTYPIC plasticity , *COLONIZATION (Ecology) , *GENE expression in bacteria - Abstract
The metabolic flexibility of the opportunistic fungal pathogen Candida albicans is important for colonisation and infection of different host niches. Complex regulatory networks, in which protein kinases play central roles, link metabolism and other virulence-associated traits, such as filamentous growth and stress resistance, and thereby control commensalism and pathogenicity. By screening a protein kinase deletion mutant library that was generated in the present work using an improved SAT1 flipper cassette, we found that the previously uncharacterised kinase Sak1 is a key upstream activator of the protein kinase Snf1, a highly conserved regulator of nutrient stress responses that is essential for viability in C. albicans. The sak1Δ mutants failed to grow on many alternative carbon sources and were hypersensitive to cell wall/membrane stress. These phenotypes were mirrored in mutants lacking other subunits of the SNF1 complex and partially compensated by a hyperactive form of Snf1. Transcriptional profiling of sak1Δ mutants showed that Sak1 ensures basal expression of glyoxylate cycle and gluconeogenesis genes even in glucose-rich media and thereby contributes to the metabolic plasticity of C. albicans. In a mouse model of gastrointestinal colonisation, sak1Δ mutants were rapidly outcompeted by wild-type cells, demonstrating that Sak1 is essential for the in vivo fitness of C. albicans. [ABSTRACT FROM AUTHOR]
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- 2017
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10. Identification of Candida glabrata Genes Involved in pH Modulation and Modification of the Phagosomal Environment in Macrophages.
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Kasper, Lydia, Seider, Katja, Gerwien, Franziska, Allert, Stefanie, Brunke, Sascha, Schwarzmüller, Tobias, Ames, Lauren, Zubiria-Barrera, Cristina, Mansour, Michael K., Becken, Ulrike, Barz, Dagmar, Vyas, Jatin M., Reiling, Norbert, Haas, Albert, Haynes, Ken, Kuchler, Karl, and Hube, Bernhard
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CANDIDA , *HYDROGEN-ion concentration , *MACROPHAGES , *INVASIVE candidiasis , *ENDOSOMES , *CELL differentiation , *CELLULAR signal transduction - Abstract
Candida glabrata currently ranks as the second most frequent cause of invasive candidiasis. Our previous work has shown that C. glabrata is adapted to intracellular survival in macrophages and replicates within non-acidified late endosomal-stage phagosomes. In contrast, heat killed yeasts are found in acidified matured phagosomes. In the present study, we aimed at elucidating the processes leading to inhibition of phagosome acidification and maturation. We show that phagosomes containing viable C. glabrata cells do not fuse with pre-labeled lysosomes and possess low phagosomal hydrolase activity. Inhibition of acidification occurs independent of macrophage type (human/murine), differentiation (M1-/M2-type) or activation status (vitamin D3 stimulation). We observed no differential activation of macrophage MAPK or NFκB signaling cascades downstream of pattern recognition receptors after internalization of viable compared to heat killed yeasts, but Syk activation decayed faster in macrophages containing viable yeasts. Thus, delivery of viable yeasts to non-matured phagosomes is likely not triggered by initial recognition events via MAPK or NFκB signaling, but Syk activation may be involved. Although V-ATPase is abundant in C. glabrata phagosomes, the influence of this proton pump on intracellular survival is low since blocking V-ATPase activity with bafilomycin A1 has no influence on fungal viability. Active pH modulation is one possible fungal strategy to change phagosome pH. In fact, C. glabrata is able to alkalinize its extracellular environment, when growing on amino acids as the sole carbon source in vitro. By screening a C. glabrata mutant library we identified genes important for environmental alkalinization that were further tested for their impact on phagosome pH. We found that the lack of fungal mannosyltransferases resulted in severely reduced alkalinization in vitro and in the delivery of C. glabrata to acidified phagosomes. Therefore, protein mannosylation may play a key role in alterations of phagosomal properties caused by C. glabrata. [ABSTRACT FROM AUTHOR]
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- 2014
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11. Cover Image: Candidalysin delivery to the invasion pocket is critical for host epithelial damage induced by Candida albicans (Cellular Microbiology 10/2021).
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Mogavero, Selene, Sauer, Frank M., Brunke, Sascha, Allert, Stefanie, Schulz, Daniela, Wisgott, Stephanie, Jablonowski, Nadja, Elshafee, Osama, Krüger, Thomas, Kniemeyer, Olaf, Brakhage, Axel A., Naglik, Julian R., Dolk, Edward, and Hube, Bernhard
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CANDIDA albicans , *MICROBIOLOGY , *EPITHELIAL cells - Published
- 2021
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