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This study examines the involvement of Wnt signaling members in the differentiation of cells related to eyespot and band color patterns in butterfly wings. The localization and function of both canonical and noncanonical Wnt signaling in Bicyclus anynana were examined to identify specific Wnt ligands and receptors essential for band development and eyespot. The findings suggest that Wnt signaling pathways play crucial roles in controlling the pattern of eyespots and bands in butterflies.

If the same pigment is found in different tissues in a body, it is natural to assume that the same metabolic pathways are deployed similarly in each tissue. Here we show that this is not the case for ommochromes, the red and orange pigments found in the eyes and wings of butterflies. We tested the expression and function of vermilion and cinnabar, two known fly genes in the ommochrome pathway, in the development of pigments in the eyes and in the wings of Bicyclus anynana butterflies, both traits having reddish/orange pigments. By using fluorescent in-situ hybridization (HCR3.0) we localized the expression of vermilion and cinnabar in the cytoplasm of pigment cells in the ommatidia but observed no clear expression for either gene on larval and pupal wings. We then disrupted the function of both genes, using CRISPR-Cas9, which resulted in the loss of pigment in the eyes but not in the wings. Using thin-layer chromatography and UV–vis spectroscopy we identified the presence of ommochrome and ommochrome precursors in the orange wing scales and in the hemolymph of pupae. We conclude that the wings either synthesize ommochromes locally, with yet unidentified enzymes or incorporate these pigments synthesized elsewhere from the hemolymph. Different metabolic pathways or transport mechanisms, thus, lead to the presence of ommochromes in the wings and eyes of B. anynana butterflies. [ABSTRACT FROM AUTHOR]

If the same pigment is found in different tissues in a body, it is natural to assume that the same metabolic pathways are deployed similarly in each tissue. Here we show that this is not the case for ommochromes, the red and orange pigments found in the eyes and wings of butterflies. We tested the expression and function of vermilion and cinnabar, two known fly genes in the ommochrome pathway, in the development of pigments in the eyes and in the wings of Bicyclus anynana butterflies, both traits having reddish/orange pigments. By using fluorescent in-situ hybridization (HCR3.0) we localized the expression of vermilion and cinnabar in the cytoplasm of pigment cells in the ommatidia but observed no clear expression for either gene on larval and pupal wings. We then disrupted the function of both genes, using CRISPR-Cas9, which resulted in the loss of pigment in the eyes but not in the wings. Using thin-layer chromatography and UV–vis spectroscopy we identified the presence of ommochrome and ommochrome precursors in the orange wing scales and in the hemolymph of pupae. We conclude that the wings either synthesize ommochromes locally, with yet unidentified enzymes or incorporate these pigments synthesized elsewhere from the hemolymph. Different metabolic pathways or transport mechanisms, thus, lead to the presence of ommochromes in the wings and eyes of B. anynana butterflies. [ABSTRACT FROM AUTHOR]

The environmental pollution, property losses and casualties caused by wildfires in California are getting worse by the year. To minimize the interference of wildfires on economic and social development, and formulate targeted mitigation strategies, it is imperative to understand the scale and extent of previous wildfire occurrences. In this study, we first investigated the temporal distributions of past wildfires in California divided by size and causes and analyzed the changes observed in the past two decades against the last century. The trend of wildfires in different time scales (yearly and monthly), as well as the distribution of wildfires across different spatial scales (administrative units, climate divisions in California from 2000 to 2019) were also studied. Furthermore, to extract the significant variables on the risk of wildfire occurrence, multivariate analyses of environmental and human-related variables with wildfire densities were carried out. The results show that the wildfire density distribution of the burned area in California conforms to the characteristics of the Pareto distribution. Over the past two decades, the frequency of small (< 500 acres), human-caused wildfires has increased most rapidly, and they are widely distributed in central and western California. The wildfire season has lengthened and the peak months have been advanced from August to July. In terms of the variables related to the risk of wildfire occurrence, the temperature, vapor pressure deficit, grass cover, and the distance to roads are crucial. This study reveals the relationship between environmental and social background conditions and the spatial-temporal distribution of wildfires, which can provide a reference for wildfire management, the formulation of future targeted wildfire emergency plans, and the planning of future land use in California. [ABSTRACT FROM AUTHOR]

The vertical turbulent transfer of heat and momentum in the lower atmospheric boundary layer is accomplished through intermittent sweep, ejection, outward interaction, and inward interaction events associated with turbulent updrafts and downdrafts. These events, collectively referred to as sweep-ejection dynamics, have been studied extensively in forested and nonforested environments and reported in the literature. However, little is known about the sweep-ejection dynamics that occur in response to turbulence regimes induced by wildland fires in forested and nonforested environments. This study attempts to fill some of that knowledge gap through analyses of turbulence data previously collected during three wildland (prescribed) fires that occurred in grassland and forested environments in Texas and New Jersey. Tower-based high-frequency (10 or 20 Hz) three-dimensional wind-velocity and temperature measurements are used to examine frequencies of occurrence of sweep, ejection, outward interaction, and inward interaction events and their actual contributions to the mean vertical turbulent fluxes of heat and momentum before, during, and after the passage of fire fronts. The observational results suggest that wildland fires in these environments can substantially change the sweep-ejection dynamics for turbulent heat and momentum fluxes that typically occur when no fires are present, especially the relative contributions of sweeps versus ejections in determining overall heat and momentum fluxes. [ABSTRACT FROM AUTHOR]

We investigate the effects of an isolated meso-γ-scale surface heterogeneity for roughness and albedo on the atmospheric boundary-layer (ABL) height, with a case study at a semi-arid forest surrounded by sparse shrubland (forest area: 28km2, forest length in the main wind direction: 7 km). Doppler lidar and ceilometer measurements at this semi-arid forest show an increase in the ABL height over the forest compared with the shrubland on four out of eight days. The differences in the ABL height between shrubland and forest are explained for all days with a model that assumes a linear growth of the internal boundary layer of the forest through the convective ABL upwind of the forest followed by a square-root growth into the stable free atmosphere. For the environmental conditions that existed during our measurements, the increase in ABL height due to large sensible heat fluxes from the forest (600Wm-2 in summer) is subdued by stable stratification in the free atmosphere above the ABL, or reduced by high wind speeds in the mixed layer. [ABSTRACT FROM AUTHOR]

Flume experiments with particle imaging velocimetry (PIV) were conducted recently to study a complex flow problem where wind shear acts on the surface of a static water body in presence of flexible emergent vegetation and induces a rich dynamics of wave–turbulence–vegetation interaction inside the water body without any gravitational gradient. The experiments were aimed at mimicking realistic vegetated wetlands and the present work is targeted to improve the understanding of the coherent structures associated with this interaction by employing a combination of techniques such as quadrant analysis, proper orthogonal decomposition (POD), Shannon entropy and mutual information content (MIC). The turbulent transfer of momentum is found to be dominated by organized motions such as sweeps and ejections, while the wave component of vertical momentum transport does not show any such preference. Reducing the data using POD shows that wave energy for large flow depths and turbulent energy for all water depths is concentrated among the top few modes, which can allow development of simple reduced order models. Vegetation flexibility is found to induce several roll type structures, however if the vegetation density is increased, drag effects dominate over flexibility and organize the flow. The interaction between waves and turbulence is also found to be highest among flexible sparse vegetation. However, rapidly evolving parts of the flow such as the air–water interface reduces wave–turbulence interaction. [ABSTRACT FROM AUTHOR]

A two-dimensional analytical model for describing the mean flow behavior inside a vegetation canopy after a leading edge in neutral conditions was developed and tested by means of large eddy simulations (LES) employing the LES code PALM. The analytical model is developed for the region directly after the canopy edge, the adjustment region, where one-dimensional canopy models fail due to the sharp change in roughness. The derivation of this adjustment region model is based on an analytic solution of the two-dimensional Reynolds averaged Navier–Stokes equation in neutral conditions for a canopy with constant plant area density (PAD). The main assumptions for solving the governing equations are separability of the velocity components concerning the spatial variables and the neglection of the Reynolds stress gradients. These two assumptions are verified by means of LES. To determine the emerging model parameters, a simultaneous fitting scheme was applied to the velocity and pressure data of a reference LES simulation. Furthermore a sensitivity analysis of the adjustment region model, equipped with the previously calculated parameters, was performed varying the three relevant length, the canopy height ( h ), the canopy length and the adjustment length ( L c ), in additional LES. Even if the model parameters are, in general, functions of h / L c , it was found out that the model is capable of predicting the flow quantities in various cases, when using constant parameters. Subsequently the adjustment region model is combined with the one-dimensional model of Massman [ Bound. Layer Meteorol. , 83(3):407–421, 1997], which is applicable for the interior of the canopy, to attain an analytical model capable of describing the mean flow for the full canopy domain. Finally the model is tested against an analytical model based on a linearization approach. [ABSTRACT FROM AUTHOR]

Under nonneutral stratification and in the presence of topography, the dynamics of turbulent flow within a canopy is not yet completely understood. This has, among other consequences, serious implications for the measurement of surface-atmosphere exchange by means of eddy covariance: for example, the measurement of carbon dioxide fluxes is strongly influenced if drainage flows occur during night, when the flow within the canopy decouples from the flow aloft. An improved physical understanding of the behavior of scalars under canopy turbulence in complex terrain is urgently needed. In the present work, the authors investigate the dynamics of turbulent flow within sloped canopies, focusing on the slope wind and potential temperature. The authors concentrate on the presence of oscillatory behavior in the flow variables in terms of switching of flow regimes by conducting linear stability analysis. The authors revisit and correct the simplified theory that exists in the literature, which is based on the interplay between the drag force and the buoyancy. The authors find that the simplified description of this dynamical system cannot exhibit the observed richness of the dynamics. To augment the simplified dynamical system's analysis, the authors make use of large-eddy simulation of a three-dimensional hill covered by a homogeneous forest and analyze the phase synchronization behavior of the buoyancy and drag forces in the momentum budget to explore the turbulent dynamics in more detail. [ABSTRACT FROM AUTHOR]

Parameterizations of biosphere-atmosphere interaction processes in climate models and other hydrological applications require characterization of turbulent transport of momentum and scalars between vegetation canopies and the atmosphere, which is often modeled using a turbulent analogy to molecular diffusion processes. Simple flux-gradient approaches (K theory) fail for canopy turbulence, however. One cause is turbulent transport by large coherent eddies at the canopy scale, which can be linked to sweep-ejection events and bear signatures of nonlocal organized eddy motions. TheKtheory, which parameterizes the turbulent flux or stress proportional to the local concentration or velocity gradient, fails to account for these nonlocal organized motions. The connection to sweep-ejection cycles and the local turbulent flux can be traced back to the turbulence triple moment c0w0w0. In this work, large-eddy simulation is used to investigate the diagnostic connection between the failure of K theory and sweep-ejection motions. Analyzed schemes are quadrant analysis and complete and incomplete cumulant expansion methods. The latter approaches introduce a turbulence time scale in the modeling. Furthermore, it is found that the momentum flux and sensible heat flux need different formulations for the turbulence time scale. Accounting for buoyancy in stratified conditions is also deemed important in addition to accounting for nonlocal events to predict the correct momentum or scalar fluxes. [ABSTRACT FROM AUTHOR]

These authors contributed equally: Shaun Hong Chuen How and Tirtha Das Banerjee. Correction to: I Scientific Reports i https://doi.org/10.1038/s41598-023-36491-9, published online 09 June 2023 The original version of this Article contained an error in the name of author Shaun Hong Chuen How, which was incorrectly given as How Hong Chuen Shaun. [Extracted from the article]

Expressions for the logarithmic variations of the normalized turbulent longitudinal velocity (u2p+)1/p with normalized distance z/δ from a boundary for high-order (p) moments in the intermediate region of wall bounded flows characterized by thickness δ are derived. The ansatz that (u2p+)1/p variation in ln(z/δ) originates from a compound effect of random sweeping and -1 power-law scaling in the longitudinal velocity spectrum Eu(k) is discussed, where k is the wavenumber. Using velocity time series sampled above a uniform ice sheet, an Eu(k) ~ k-1 scaling is confirmed for k z < 1 and kδ > 1. The data were then used to analyze assumptions required for the utility of the random sweeping decorrelation (RSD) hypothesis connecting the k-1 power-law with log-scaling in (u2p+)1/p. It has been found out that while the RSD hypothesis is operationally applicable to scales associated with attached eddies bounded by kz < 1 and kδ > 1, significant interactions among high-order turbulent velocity and velocity increments lead to the conclusion that the RSD hypothesis cannot be exactly valid. Its operational utility stems from the observations that some of the interaction terms among the high-order velocity and velocity increments act in opposite directions thereby canceling their additive effects in RSD. [ABSTRACT FROM AUTHOR]

A spectral budget model is developed to describe the scaling behavior of the longitudinal turbulent velocity variance with the stability parameter and the normalized height in an idealized stably stratified atmospheric surface layer (ASL), where z is the height from the surface, L is the Obukhov length, and δ is the boundary layer height. The proposed framework employs Kolmogorov's hypothesis for describing the shape of the longitudinal velocity spectra in the inertial subrange, Heisenberg's eddy viscosity as a closure for the pressure redistribution and turbulent transfer terms, and the Monin-Obukhov similarity theory (MOST) scaling for linking the mean longitudinal velocity and temperature profiles to ζ. At a given friction velocity , reduces with increasing ζ as expected. The model is consistent with the disputed z-less stratification when the stability correction function for momentum increases with increasing ζ linearly or as a power law with the exponent exceeding unity. For the Businger-Dyer stability correction function for momentum, which varies linearly with ζ, the limit of the z-less onset is . The proposed framework explains why does not follow MOST scaling even when the mean velocity and temperature profiles may follow MOST in the ASL. It also explains how δ ceases to be a scaling variable in more strongly stable (although well-developed turbulent) ranges. [ABSTRACT FROM AUTHOR]

The problem of wind-induced flow in inland waters is drawing significant research attention given its relevance to a plethora of applications in wetlands including treatment designs, pollution reduction, and biogeochemical cycling. The present work addresses the role of wind induced turbulence and waves within an otherwise static water body in the presence of rigid and flexible emergent vegetation through flume experimentation and time series analysis. Because no prior example of Particle Imaging Velocimetry (PIV) experiments involving air–water and flexible oscillating components have been found in the literature, a spectral analysis framework is needed and proposed here to guide the analysis involving noise, wave and turbulence separation. The experiments reveal that wave and turbulence effects are simultaneously produced at the air–water interface and the nature of their coexistence is found to vary with different flow parameters including water level, mean wind speed, vegetation density and its flexibility. For deep water levels, signature of fine-scaled inertial turbulence is found at deeper layers of the water system. The wave action appears stronger close to the air–water interface and damped by the turbulence deeper inside the water system. As expected, wave action is found to be dominated in a certain frequency range driven by the wind forcing, while it is also diffused to lower frequencies by means of (wind-induced) oscillations in vegetation. Regarding the mean water velocity, existence of a counter-current flow and its switching to fully forward flow in the direction of the wind under certain combinations of flow parameters were studied. The relative importance of wave and turbulence to the overall energy, degree of anisotropy in the turbulent energy components, and turbulent momentum transport at different depths from the air–water interface and flow combinations were then quantified. The flume experiments reported here differ from previous laboratory studies in the related literature involving vegetation in the sense that the wave forcing is only present on the water surface contrary to a full-body excitation by tidal wave simulators and thus important in advancing the knowledge regarding a wider range of water resource problems. [ABSTRACT FROM AUTHOR]

A streamfunction-vorticity formulation is used to explore the extent to which turbulent and turbulently inviscid solutions to the mean momentum balance explain the mean flow across forest edges and within cavities situated inside dense forested canopies. The turbulent solution is based on the mean momentum balance where first-order closure principles are used to model turbulent stresses. The turbulently inviscid solution retains all the key terms in the mean momentum balance but for the turbulent stress gradients. Both exit and entry versions of the forest edge problem are explored. The turbulent solution is found to describe sufficiently the bulk spatial patterns of the mean flow near the edge including signatures of different length scales reported in canopy transition studies. Next, the 'clearing inside canopy' or the so-called 'cavity' problem is solved for the inviscid and turbulent solutions and then compared against flume experiments. The inviscid solution describes the bulk flow dynamics in much of the zones within the cavity. In particular, the solution can capture the correct position of the bulk recirculation zone within the cavity, although with a weaker magnitude. The inviscid solution cannot capture the large vertical heterogeneity in the mean velocity above the canopy, as expected. These features are better captured via the first-order closure representation of the turbulent solution. Given the ability of this vorticity formulation to capture the mean pressure variations and the mean advective acceleration terms, it is ideal for exploring the distributions of scalars and roughness-induced flow adjustments on complex topography. [ABSTRACT FROM AUTHOR]

Purpose: Coronavirus outbreak was first reported towards the end of 2019 and has now been declared a pandemic by the World Health Organization. Post declaration, India announced lockdown and various guidelines were issued by Health authorities worldwide for the organizations to function in this emergency aeon. Different organizations strategized differently to respond to COVID 19 pandemic. The aim was to implement COVID 19 guidelines across all the centres of a chain of Eye hospitals in India in short span of time overcoming the challenges.Methods: A SMART strategy was confabulated within the team. There were numerous challenges like lack of time, rapidly changing guidelines, no travelling scenarios, training and educating the staff in huge numbers, rapidly evolving situation, change management, need of increased vigilance, and language barrier at certain centres, sustaining quality assurance and motivating the staff in stressful times. The challenges were surpassed using a power packed strategy, dedicated efforts and team work grasping the available tools and technology from a single location. This template can serve us and other hospitals to respond in a better way for similar future challenges.Results: The COVID 19 protocols were implemented across all the centres successfully. The entire Quality assurance system drifted to online platform and education and awareness methods took the digital thread. Teamwork synergized with the aforementioned things and we came out with flying colours achieving the goals in short span of time overcoming all the barriers.Conclusion: Difficult times bring challenges and challenges teach us the innovative ways to convert them into opportunities. A well planned strategy, best utilization of available resources, team work and continuous monitoring can bring the best outcomes forward. [ABSTRACT FROM AUTHOR]

Increasing trends in wildfire severity can partly be attributed to fire exclusion in the past century which led to higher fuel accumulation. Mechanical thinning and prescribed burns are effective techniques to manage fuel loads and to establish a higher degree of control over future fire risk, while restoring fire prone landscapes to their natural states of succession. However, given the complexity of interactions between fine scale fuel heterogeneity and wind, it is difficult to assess the success of thinning operations and prescribed burns. The present work addresses this issue systematically by simulating a simple fire line and propagating through a vegetative environment where the midstory has been cleared in different degrees, leading to a canopy with almost no midstory, another with a sparse midstory and another with a dense midstory. The simulations are conducted for these three canopies under two different conditions, where the fuel moisture is high and where it is low. These six sets of simulations show widely different fire behavior, in terms of fire intensity, spread rate and consumption. To understand the physical mechanisms that lead to these differences, detailed analyses are conducted to look at wind patterns, mean flow and turbulent fluxes of momentum and energy. The analyses also lead to improved understanding of processes leading to high intensity crowning behavior in presence of a dense midstory. Moreover, this work highlights the importance of considering fine scale fuel heterogeneity, seasonality, wind effects and the associated fire-canopy-atmosphere interactions while considering prescribed burns and forest management operations. [ABSTRACT FROM AUTHOR]

High frequency (30 Hz) two-dimensional particle image velocimetry data recorded during a field experiment exploring fire spread from point ignition in hand-spread pine needles under calm ambient wind conditions are analysed in this study. In the initial stages, as the flame spreads approximately radially away from the ignition point in the absence of a preferred wind-forcing direction, it entrains cooler ambient air into the warmer fire core, thereby experiencing a dynamic pressure resistance. The fire-front, comprising a flame that is tilted inward, is surrounded by a region of downdraft. Coherent structures describe the initial shape of the fire-front and its response to local wind shifts while also revealing possible fire-spread mechanisms. Vortex tubes originating outside the fire spiral inward and get stretched thinner at the fire-front leading to higher vorticity there. These tubes comprise circulation structures that induce a radially outward velocity close to the fuel bed, which pushes hot gases outward, thereby causing the fire to spread. Moreover, these circulation structures confirm the presence of counter-rotating vortex pairs that are known to be a key mechanism for fire spread. The axis of the vortex tubes changes its orientation alternately towards and away from the surface of the fuel bed, causing the vortex tubes to be kinked. The strong updraft observed at the location of the fire-front could potentially advect and tilt the kinked vortex tube vertically upward leading to fire-whirl formation. As the fire evolves, its perimeter disintegrates in response to flow instabilities to form smaller fire "pockets". These pockets are confined to certain points in the flow field that remain relatively fixed for a while and resemble the behavior of a chaotic system in the vicinity of an attractor. Increased magnitudes of the turbulent fluxes of horizontal momentum, computed at certain such fixed points along the fire-front, are symptomatic of irregular fire bursts and help contextualize the fire spread. Most importantly, the time-varying transport terms of the turbulent kinetic energy budget equation computed at adjacent fixed points indicate that local fires along the fire-front primarily interact via the horizontal turbulent transport term. [ABSTRACT FROM AUTHOR]

Wildfire behavior is dictated by the complex interaction of numerous physical phenomena including dynamic ambient and fire-induced winds, heat transfer, aerodynamic drag on the wind by the fuel and combustion. These phenomena create complex feedback effects between the fire and its surroundings. In this study, we aim to study the mechanisms by which buoyant flame dynamics along with vortical motions and instabilities control wildfire propagation. Specifically, this study employs a suite of simulations conducted with the physics-based coupled fire-atmosphere behavior model (FIRETEC). The simulations are initialized with a fire line and the fires are allowed to propagate on a grass bed, where the fuel heights and wind conditions are varied systematically. Flow variables are extracted to identify the characteristics of the alternating counter-rotational vortices, called towers and troughs, that drive convective heat transfer and fire spread. These vortices have previously been observed in wildfires and laboratory fires, and have also been observed to arise spontaneously in FIRETEC due to the fundamental physics incorporated in the model. However, these past observations have been qualitative in nature and no quantitative studies can be found in the literature which connected these coherent structures fundamental to fire behavior with the constitutive flow variables. To that end, a variety of state variables are examined in the context of these coherent structures under various wind profile and grass height conditions. Identification of various correlated signatures and fire-atmosphere feedbacks in simulations provides a hypothesis that can be tested in future observational or experimental efforts, potentially assisting experimental design, and can aid in the interpretation of data from in situ detectors. [ABSTRACT FROM AUTHOR]

Wildland fire–atmosphere interaction generates complex turbulence patterns, organized across multiple scales, which inform fire-spread behaviour, firebrand transport, and smoke dispersion. Here, we utilize wavelet-based techniques to explore the characteristic temporal scales associated with coherent patterns in the measured temperature and the turbulent fluxes during a prescribed wind-driven (heading) surface fire beneath a forest canopy. We use temperature and velocity measurements from tower-mounted sonic anemometers at multiple heights. Patterns in the wavelet-based energy density of the measured temperature plotted on a time–frequency plane indicate the presence of fire-modulated ramp–cliff structures in the low-to-mid-frequency band (0.01–0.33 Hz), with mean ramp durations approximately 20% shorter and ramp slopes that are an order of magnitude higher compared to no-fire conditions. We then investigate heat- and momentum-flux events near the canopy top through a cross-wavelet coherence analysis. Briefly before the fire-front arrives at the tower base, momentum-flux events are relatively suppressed and turbulent fluxes are chiefly thermally-driven near the canopy top, owing to the tilting of the flame in the direction of the wind. Fire-induced heat-flux events comprising warm updrafts and cool downdrafts are coherent down to periods of a second, whereas ambient heat-flux events operate mainly at higher periods (above 17 s). Later, when the strongest temperature fluctuations are recorded near the surface, fire-induced heat-flux events occur intermittently at shorter scales and cool sweeps start being seen for periods ranging from 8 to 35 s near the canopy top, suggesting a diminishing influence of the flame and increasing background atmospheric variability thereat. The improved understanding of the characteristic time scales associated with fire-induced turbulence features, as the fire-front evolves, will help develop more reliable fire behaviour and scalar transport models. [ABSTRACT FROM AUTHOR]

Mesoscale‐to‐microscale coupling is an important tool for conducting turbulence‐resolving multiscale simulations of realistic atmospheric flows, which are crucial for applications ranging from wind energy to wildfire spread studies. Different techniques are used to facilitate the development of realistic turbulence in the large‐eddy simulation (LES) domain while minimizing computational cost. Here, we explore the impact of a simple and computationally efficient Stochastic Cell Perturbation method using momentum perturbation (SCPM‐M) to accelerate turbulence generation in boundary‐coupled LES simulations using the Weather Research and Forecasting model. We simulate a convective boundary layer (CBL) to characterize the production and dissipation of turbulent kinetic energy (TKE) and the variation of TKE budget terms. Furthermore, we evaluate the impact of applying momentum perturbations of three magnitudes below, up to, and above the CBL on the TKE budget terms. Momentum perturbations greatly reduce the fetch associated with turbulence generation. When applied to half the vertical extent of the boundary layer, momentum perturbations produce an adequate amount of turbulence. However, when applied above the CBL, additional structures are generated at the top of the CBL, near the inversion layer. The magnitudes of the TKE budgets produced by SCPM‐M when applied at varying heights and with different perturbation amplitudes are always higher near the surface and inversion layer than those produced by No‐SCPM, as are their contributions to the TKE. This study provides a better understanding of how SCPM‐M reduces computational costs and how different budget terms contribute to TKE in a boundary‐coupled LES simulation. Plain Language Summary: Grid nesting is a technique in atmospheric modeling where smaller model domains with finer resolutions are embedded in larger domains with coarser resolutions, allowing us to simulate processes for which a range of resolutions is important. When nesting a micro‐scale domain, which resolves atmospheric turbulence, within a mesoscale domain, where turbulence is parameterized instead, the micro‐scale domain needs to generate turbulence. This can take a long distance, reducing the area within the micro‐scale domain where turbulence is fully developed. Turbulence generation methods can speed up this process, leading to more efficient multi‐scale simulations. Here, we take a close look at one such turbulence generation method, which applies random perturbations to the momentum field along the boundaries of the micro‐scale domain. We test several different configurations of momentum perturbations and evaluate their impacts on the turbulent kinetic energy budget within the micro‐scale domain. Our results can be used as guidance on how to apply momentum perturbations most efficiently in grid‐nested simulations. Key Points: The fetch is reduced after applying momentum perturbation and the reduction is directly proportional to the amplitude of the perturbationsApplying momentum perturbation up to half the boundary layer height produces an adequate amount of turbulence without additional entrainmentMomentum perturbations result in turbulent kinetic energy budget terms of higher magnitude than simulations without perturbations [ABSTRACT FROM AUTHOR]

The characterization of heat and momentum fluxes in wall-bounded turbulence is of paramount importance for a plethora of applications ranging from engineering to Earth sciences. Nevertheless, how the turbulent structures associated with velocity and temperature fluctuations interact to produce the emergent flux signatures has not been evident until now. In this work, we investigate this fundamental issue by studying the switching patterns of intermittently occurring turbulent fluctuations from one state to another, a phenomenon called persistence. We discover that the persistence patterns for heat and momentum fluxes are widely different. Moreover, we uncover power-law scaling and length scales of turbulent motions that cause this behavior. Furthermore, by separating the phases and amplitudes of flux events, we explain the origin and differences between heat and momentum transfer efficiencies in convective turbulence. Our findings provide a new understanding of the connection between flow organization and flux generation mechanisms, two cornerstones of turbulence research. [ABSTRACT FROM AUTHOR]

Persistence is defined as the probability that the local value of a fluctuating field remains at a particular state for a certain amount of time, before being switched to another state. The concept of persistence has been found to have many diverse practical applications, ranging from non-equilibrium statistical mechanics to financial dynamics to distribution of time scales in turbulent flows and many more. In this study, we carry out a detailed analysis of the statistical characteristics of the persistence probability density functions (PDFs) of velocity and temperature fluctuations in the surface layer of a convective boundary layer using a field-experimental dataset. Our results demonstrate that for the time scales smaller than the integral scales, the persistence PDFs of turbulent velocity and temperature fluctuations display a clear power-law behavior, associated with a self-similar eddy cascading mechanism. Moreover, we also show that the effects of non-Gaussian temperature fluctuations act only at those scales that are larger than the integral scales, where the persistence PDFs deviate from the power-law and drop exponentially. Furthermore, the mean time scales of the negative temperature fluctuation events persisting longer than the integral scales are found to be approximately equal to twice the integral scale in highly convective conditions. However, with stability, this mean time scale gradually decreases to almost being equal to the integral scale in the near-neutral conditions. Contrarily, for the long positive temperature fluctuation events, the mean time scales remain roughly equal to the integral scales, irrespective of stability. [ABSTRACT FROM AUTHOR]

Franconibacter pulveris strain DJ34, isolated from Duliajan oil fields, Assam, was characterized in terms of its taxonomic, metabolic and genomic properties. The bacterium showed utilization of diverse petroleum hydrocarbons and electron acceptors, metal resistance, and biosurfactant production. The genome (4,856,096 bp) of this strain contained different genes related to the degradation of various petroleum hydrocarbons, metal transport and resistance, dissimilatory nitrate, nitrite and sulfite reduction, chemotaxy, biosurfactant synthesis, etc. Genomic comparison with other Franconibacter spp. revealed higher abundance of genes for cell motility, lipid transport and metabolism, transcription and translation in DJ34 genome. Detailed COG analysis provides deeper insights into the genomic potential of this organism for degradation and survival in oil-contaminated complex habitat. This is the first report on ecophysiology and genomic inventory of Franconibacter sp. inhabiting crude oil rich environment, which might be useful for designing the strategy for bioremediation of oil contaminated environment. [ABSTRACT FROM AUTHOR]

Arsenic (As) biotransformation and release by indigenous bacteria from As rich groundwater was investigated. Metabolic landscape of 173 bacterial isolates indicated broad catabolic repertoire including abundance of As 5+ reductase activity and abilities in utilizing wide ranges of organic and inorganic respiratory substrates. Abundance of As homeostasis genes and utilization of hydrocarbon as carbon/electron donor and As 5+ as electron acceptor were noted within the isolates. Sediment microcosm study (for 300 days) showed a pivotal role of metal reducing facultative anaerobic bacteria in toxic As 3+ release in aqueous phase. Inhabitant bacteria catalyze As transformation and facilitate its release through a cascade of reactions including mineral bioweathering and As 5+ and/or Fe 3+ reduction activities. Compared to anaerobic incubation with As 5+ reducing strains, oxic state and/or incubation with As 3+ oxidizing bacteria resulted in reduced As release, thus indicating a strong role of such condition or biocatalytic mechanism in controlling in situ As contamination. [ABSTRACT FROM AUTHOR]

Due to the mixed distribution of buildings and vegetation, wildland-urban interface (WUI) areas are characterized by complex fuel distributions and geographical environments. The behavior of wildfires occurring in the WUI often leads to severe hazards and significant damage to man-made structures. Therefore, WUI areas warrant more attention during the wildfire season. Due to the ever-changing dynamic nature of California's population and housing, the update frequency and resolution of WUI maps that are currently used can no longer meet the needs and challenges of wildfire management and resource allocation for suppression and mitigation efforts. Recent developments in remote sensing technology and data analysis algorithms pose new opportunities for improving WUI mapping methods. WUI areas in California were directly mapped using building footprints extracted from remote sensing data by Microsoft along with the fuel vegetation cover from the LANDFIRE dataset in this study. To accommodate the new type of datasets, we developed a threshold criteria for mapping WUI based on statistical analysis, as opposed to using more ad-hoc criteria as used in previous mapping approaches. This method removes the reliance on census data in WUI mapping, and does not require the calculation of housing density. Moreover, this approach designates the adjacent areas of each building with large and dense parcels of vegetation as WUI, which can not only refine the scope and resolution of the WUI areas to individual buildings, but also avoids zoning issues and uncertainties in housing density calculation. Besides, the new method has the capability of updating the WUI map in real-time according to the operational needs. Therefore, this method is suitable for local governments to map local WUI areas, as well as formulating detailed wildfire emergency plans, evacuation routes, and management measures. [ABSTRACT FROM AUTHOR]

Quantifying livelihood vulnerability to wildland fires in the United States is challenging because of the need to systematically integrate multidimensional variables into its analysis. We aim to measure wildfire threats amongst humans and their physical and social environment by developing a framework to calculate the livelihood vulnerability index (LVI) for the top 14 American states most recently exposed to wildfires. The LVI is computed by assessing each state's contributing factors (exposure, sensitivity, and adaptive capacity) to wildfire events. These contributing factors are determined through a set of indicator variables that are categorized into corresponding groups to produce an LVI framework. The framework is validated by performing a principal component analysis (PCA), ensuring that each selected indicator variable corresponds to the correct contributing factor. Our results indicate that Arizona and New Mexico experience the greatest livelihood vulnerability. In contrast, California, Florida, and Texas experience the least livelihood vulnerability. While California has one of the highest exposures and sensitivity to wildfires, results indicate that it has a relatively high adaptive capacity, in comparison to the other states, suggesting it has measures in place to withstand these vulnerabilities. These results are critical to wildfire managers, government, policymakers, and research scientists for identifying and providing better resiliency and adaptation measures to support states that are most vulnerable to wildfires. [ABSTRACT FROM AUTHOR]

Afforestation in semi-arid regions can potentially enhance the global carbon sink by increasing the terrestrial biomass. However, the survival of planted forests under such extreme environmental conditions is not guaranteed a priori, and critically depends on the surface-atmosphere exchange of energy. We investigate the pine forest Yatir in Israel, an example of a man-made semi-arid ecosystem, by means of large-eddy simulations. We focus on the interaction between surface-atmosphere exchange and secondary circulations that couple the isolated forest to the surrounding shrubland. The large-eddy simulations feature a grid resolution that resolves the forest canopy in several layers, and are initialized by satellite data and Doppler lidar, eddy-covariance and radiosonde measurements. We perform three large-eddy simulations with different geostrophic wind speeds to investigate the influence of those wind speeds on the surface-atmosphere exchange. We reproduce the measured mean updrafts above the forest and mean downdrafts above the shrubland, which increase in strength with decreasing geostrophic wind speed. The largest updrafts emerge above the older, denser part of the forest, triggering secondary circulations. The spatial extent of these circulations does not cover the entire forest area, although we observe a reduced aerodynamic resistance in the regions of updraft. Our simulations indicate that the enhanced surface-atmosphere exchange of the Yatir forest is not sufficient to compensate for the increased net radiation, due to the lower albedo of the forest with respect to the surroundings, resulting in higher air temperatures inside the forest. However, the difference between the forest and shrubland temperatures decreases with increasing geostrophic wind speed due to reduction in the aerodynamic resistance. [ABSTRACT FROM AUTHOR]

Fire-induced turbulence and the feedback into the fire, following ambient changes, differ for forested (sub-canopy) and grassland environments. Here, we synthesize observations from multiple experimental surface fires: two sub-canopy backing fires, one sub-canopy heading fire, and a grassland heading fire. We identify and compare the most essential coherent structures and processes of each case from the turbulent momentum fluxes and turbulent kinetic energy (TKE) budget terms. In the sub-canopy burns, turbulent eddies are strongest near the canopy top: high streamwise turbulent flux accompanies low cross-stream turbulent flux and vice versa. In the grassland fire, both streamwise and cross-stream eddies strengthen simultaneously until a certain height, informing a vertical length scale for the fire-influence. Moreover, the forward sweep from streamwise eddies assists in the fire spread by pushing hot gases towards unburnt fuel. In the sub-canopy fires, shear production and buoyancy production are more substantial near the canopy top for more intense fires, while their magnitudes decrease with decreasing fire intensity. At mid-canopy-height scales, buoyancy production dominates shear production, becoming the key mechanism for vertical transport of TKE. In the grassland fire, shear production dominates buoyancy production near the surface and is insignificant beyond a certain height relative to the flame length, while buoyancy production increases with height, becoming substantial further away from the surface. Turbulent transport terms are also active in both environments. For intense sub-canopy fires, there is a loss in TKE due to its expulsion to the boundary layer aloft via the transport term, compensated by a reversal process: TKE influx via the transport term. In the grassland fire, the transport term mimics this behavior until a certain height. The insights into the relative significance of the respective turbulent fluxes and TKE budget terms in each environment can help simplify the complex system of equations governing fire physics. • Excessive mid-canopy buoyancy production is key mechanism for vertical TKE transport. • Near-canopy-top streamwise eddies impede/assist fire-spread in backing/heading fires. • TKE loss in sub-canopy fires later compensated by TKE gain through transport term. • Height of strongest grassland fire eddies determines vertical length scale. • Gravitational acceleration and buoyant updrafts control sub-canopy MKE tendency. [ABSTRACT FROM AUTHOR]

• Atmospheric boundary layer observations at flux tower sites open new research opportunities. • ABL observations help interpreting land-atmosphere feedbacks. • Quality control of eddy covariance fluxes can be enhanced. • ABL observations link flux tower observations to regional-scale flux upscaling. • Quantifying land-atmosphere coupling at flux tower sites requires ABL observations. The atmospheric boundary layer mediates the exchange of energy, matter, and momentum between the land surface and the free troposphere, integrating a range of physical, chemical, and biological processes and is defined as the lowest layer of the atmosphere (ranging from a few meters to 3 km). In this review, we investigate how continuous, automated observations of the atmospheric boundary layer can enhance the scientific value of co-located eddy covariance measurements of land-atmosphere fluxes of carbon, water, and energy, as are being made at FLUXNET sites worldwide. We highlight four key opportunities to integrate tower-based flux measurements with continuous, long-term atmospheric boundary layer measurements: (1) to interpret surface flux and atmospheric boundary layer exchange dynamics and feedbacks at flux tower sites, (2) to support flux footprint modelling, the interpretation of surface fluxes in heterogeneous and mountainous terrain, and quality control of eddy covariance flux measurements, (3) to support regional-scale modeling and upscaling of surface fluxes to continental scales, and (4) to quantify land-atmosphere coupling and validate its representation in Earth system models. Adding a suite of atmospheric boundary layer measurements to eddy covariance flux tower sites, and supporting the sharing of these data to tower networks, would allow the Earth science community to address new emerging research questions, better interpret ongoing flux tower measurements, and would present novel opportunities for collaborations between FLUXNET scientists and atmospheric and remote sensing scientists. [ABSTRACT FROM AUTHOR]