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Background: Hypoglycaemia and hyperglycaemia may develop during anaesthesia and surgery in children and can lead to severe adverse clinical outcomes. No study, as far as we know, has investigated glucose homeostasis in children undergoing surgery in Malawi. The aim of this study was to assess perioperative glucose levels of the children undergoing anaesthesia at Mercy James Centre (MJC) for Paediatric Surgery, Blantyre, Malawi. Methodology: This was an observational cross-sectional study. We looked at 100 children aged 1 day to 15 years anaesthetised at MJC. Data were analysed using SPSS 28. Student t test and Analysis of the variance (ANOVA) were used to compare means. The level of significance was 5%. Results: Male children represented 68%. The median age was 2.2 years. Sixten percents of patient were underweight. Fasting times were prolonged for 87%. Maintenance IV fluid with 2.5% dextrose was given to 14%. Overall, there was a significant increase of glycaemia from induction of anaesthesia to the end of the procedure. Hypoglycaemia was rare. The mean fasting glycaemia was 99.04 mg/dL ± 1.8, 116.95 mg/dL ± 34.2 at 30 min into the procedure and 127.62 mg/dL ± 46.8 at the end of the procedure. The differences in means were statistically significant (p < 0.001). Prolonged fasting times was associated with lower blood glucose means whereas nutrition status, type of the procedure, addition of dextrose in the fluid, and duration of procedure were associated with higher glycaemia means. Conclusion: Glycaemia increases under anaesthesia and surgery. Recommended fasting times, optimising nutritional status, when possible, no dextrose or lower than 2.5% dextrose in IV maintenance fluid are possible strategies to maintain blood sugar homeostasis during paediatric surgery and anaesthesia. [ABSTRACT FROM AUTHOR]

Disease resistant crop varieties are important for both disease control and for reducing chemical pesticide use. However, there is often a trade-off between disease resistance and yield. While the European Union has banned the use of some pesticides in agriculture, large amounts are still applied to various crops, including wheat. Market-based instruments could motivate farmers to adopt more environmentally friendly production methods. In this study we aimed to explore farmers' adoption of resistant varieties in different wheat price scenarios and their willingness to forgo yield when cultivating more resistant varieties. We conducted face-to-face interviews with 192 farmers using a choice experiment. Results showed that farmers preferred traits of yield stability (fungal disease resistance, lodging resistance and drought tolerance) over yield. Preferences for traits varied with the production system; livestock production was negatively associated with the importance of yield; cultivated land area was positively associated with protein content. The market price scenario did not change preferences. We calculate that farmers are willing to forgo more yield by cultivating highly disease resistant varieties than they are likely to recuperate from reducing costs of pesticides. Overall, we conclude that farmers' choices are based more on a combination of the production system, drought experience, climate change belief and the potential impact of further regulations to reduce the use of chemical pesticides than on the expected wheat price. [ABSTRACT FROM AUTHOR]

Background: General anaesthesia (GA) in developing countries is still a high-risk practice, especially in Africa, accompanied with high morbidity and mortality. No study has yet been conducted in Butembo in the Democratic Republic of the Congo to determine the mortality related to GA practice. The main objective of this study was to assess mortality related to GA in Butembo. Methods: This was a retrospective descriptive and analytic study of patients who underwent surgery under GA in the 2 main teaching hospitals of Butembo from January 2011 to December 2015. Data were collected from patients files, anaesthesia registries and were analysed with SPSS 26. Results: From a total of 921 patients, 539 (58.5%) were male and 382 (41.5%) female patients. A total of 83 (9.0%) patients died representing an overall perioperative mortality rate of 90 per 1000. Out of the 83 deaths, 38 occurred within 24 h representing GA related mortality of 41 per 1000. There was a global drop in mortality from 2011 to 2015. The risk factors of death were: being a neonate or a senior adult, emergency operation, ASA physical status > 2 and a single deranged vital sign preoperatively, presenting any complication during GA, anaesthesia duration > 120 minutes as well as visceral surgeries/laparotomies. Ketamine was the most employed anaesthetic. Conclusion: GA related mortality is very high in Butembo. Improved GA services and outcomes can be obtained by training more anaesthesia providers, proper patients monitoring, improved infrastructure, better equipment and drugs procurement and considering regional anaesthesia whenever possible. [ABSTRACT FROM AUTHOR]

Background: Standard monitoring during anaesthesia is a core element of patient safety and practice of safe anesthesia has reduced morbidity and mortality worldwide. The main objective of this study was to assess the practice of standard monitoring during anaesthesia in the hospitals of North Kivu, so as to establish a baseline overview of the situation, and orientate plans towards safe anaesthesia in the region.Methods: This study was a cross-sectional survey of health facilities of the Health Antenna of Butembo in Democratic Republic of Congo and was conducted from October to December 2018. Questionnaires were brought to anaesthesia providers in the health facilities. The study included 1 answer from the anaesthesia practitioners who accepted to participate. The practices of standard monitoring in the health facilities were compared to WHO-WSFA guidelines. Data was captured and analyzed with Epi Info 7.Results: Forty out of 90 health facilities (44.4%) of 10 health zones responded on the questionnaire. Twenty-three health facilities (57.5%) were from private sector and 17 (42.5%) from public sector. Sixteen health facilities (40.0%) were from the Butembo health zone. The median number of anaesthesia providers was 2 per health facility. Of all the anaesthesia providers, none were physicians, 47.5% were nurses practicing anaesthesia without any training in anaesthesia and 47.5% were nurse anaesthetists. All the health facilities were providing general anaesthesia whereas spinal anaesthesia was provided in 22 out of 40 centers (55%). Seventy percent (28/40) of the facilities were below standard according to WHO-WSFA guidelines. Only 40% (16/40) were using a pulse oximeter and 10% (4/40) declared that ECG was occasionally used.Conclusion: The practice of standard monitoring is poor in health facilities of the Health Antenna of Butembo. Efforts should be made to improve monitoring which is a key element of safe anaesthesia. [ABSTRACT FROM AUTHOR]

The study starts from a hypothesis on the coherence and compatibility of the legal instruments in force in Cameroon and in the Democratic Republic of Congo (DRC) with the optimization of payment for environmental services (PES) and the preservation of environmental services. This study has employed a legal approach and interviews in order to investigate whether there is coherence and compatibility or not between the two variables of this hypothesis: (1) the legal instruments in force in Cameroon and in the DRC and (2) the optimization of PES and the preservation of environmental services. This study consists of three parts. The first part deals with the theoretical framework of PES and the place of PES in the legal order. In this first part, the definition of an optimal PES, the indicators of an optimal PES and the categories of legal frameworks on PES have been revealed. These are the fundamental elements to conduct a systematic analysis in the second and third parts. Based on these fundamental elements, the study analyses the legal instruments from international, regional (Africa) and domestic (Cameroon and the DRC) levels and investigates through field research two PES projects, one in Cameroon called "PES comminatory project" and another in the DRC called "REDD CBFF-Luki" respectively in the second and the third parts. From the analysis of these legal instruments and the investigation of these two PES projects, it has become apparent that there is a lack of coherence and compatibility between the legal instruments and these two PES projects in Cameroon and in the DRC, and the optimization of PES and the preservation of environmental services in the Congo Basin in general, especially in these two States. Useful recommendations have been made to eradicate these shortcomings. [ABSTRACT FROM AUTHOR]

Polyamine and ethylene pathway genes (PEGs) are widely involved in regulating plant abiotic stresses. However, the PEGs in Cleistogenes songorica have not yet been thoroughly studied. In the present study, 17 polyamine-, 12 ethylene- and 6 S-adenosylmethionine (SAM)- related genes were identified at the genome-wide level in C. songorica. Phylogenetic analysis showed that genes in the ethylene and polyamine pathways and SAM family genes were not clearly separated. Promoter regions of these genes were rich in stress-related cis -regulatory elements. Several PEG and SAM genes coexpressed together and were responsive to abiotic stress treatment. In particular, qRT-PCR analysis showed that the expression of CsSAMDC2 increased under salt, dehydration and ABA treatment conditions. Arabidopsis plants transformed with CsSAMDC2 driven by a stress-inducible RD29A or constitutive promoter 35S exhibited enhanced dehydration tolerance and maintained higher chlorophyll content and photosynthetic capacity. The expression of CsSAMDC2 was generally higher in lines harboring RD29A::CsSAMDC2 compared to 35 S::CsSAMDC2 lines under drought stress treatment. Seed germination of RD29A::CsSAMDC2 or 35 S::CsSAMDC2 transgenic plants under salt stress treatment was superior to their wild-type counterparts, and the leaves of the transgenic plants exhibited a more compact structure. In addition, under salt stress, application of ABA improved lateral root development of RD29A::CsSAMDC2 Arabidopsis plants. The qRT-PCR analysis indicated that the expression of well-known abiotic stress-responsive genes AtPEGs, AtERF1 and AtRD29A, increased in both RD29A::CsSAMDC2 and 35 S::CsSAMDC2 transgenic Arabidopsis after drought, salt stress and salt stress plus ABA treatments. Our results suggest that CsSAMDC2 enhances drought and salt tolerance in Arabidopsis and therefore may contribute to abiotic stress tolerance in forage and food crops. • Genome wide analysis of ethylene and polyamine pathway and SAM family genes in C. songorica. • Evolutionary and functional similarities between ethylene and polyamine pathway genes. • CsSAMDC2 driven by RD29A promoter improved drought and salt tolerance of transgenic Arabidopsis. • Application of ABA on transgenic lines under salt stress further improved the expression of polyamine and ethylene pathway genes. [ABSTRACT FROM AUTHOR]

In this paper, we propose a new methodology to conceive a thinning scheme based on the parallel deletion of P-simple points. This scheme needs neither a preliminary labelling nor an extended neighborhood, in the opposite of the already proposed thinning algorithms based on P-simple points. Moreover, from an existent thinning algorithm A, we construct another thinning algorithm A′, such that A′ deletes at least all the points removed by A, while preserving the same end points. In fact, we propose a 12-subiteration thinning algorithm which deletes at least the points removed by the one proposed by Pala´gyi and Kuba (Graphical Models Image Process. 61 (1999) 199). [Copyright &y& Elsevier]

An amendment to this paper has been published and can be accessed via the original article. [ABSTRACT FROM AUTHOR]

Plant growth and development depends on its ability to maintain optimal cellular homeostasis during abiotic and biotic stresses. Cleistogenes songorica, a xerophyte desert plant, is known to have novel drought stress adaptation strategies and contains rich pools of stress tolerance genes. Proteins encoded by Late Embryogenesis Abundant (LEA) family genes promote cellular activities by functioning as disordered molecules, or by limiting collisions between enzymes during stresses. To date, functions of the LEA family genes have been heavily investigated in many plant species except perennial monocotyledonous species. In this study, 44 putative LEA genes were identified in the C. songorica genome and were grouped into eight subfamilies, based on their conserved protein domains and domain organizations. Phylogenetic analyses indicated that C. songorica Dehydrin and LEA_2 subfamily proteins shared high sequence homology with stress responsive Dehydrin proteins from Arabidopsis. Additionally, promoter regions of CsLEA_2 or CsDehydrin subfamily genes were rich in G-box, drought responsive (MBS), and/or Abscisic acid responsive (ABRE) cis-regulatory elements. In addition, gene expression analyses indicated that genes from these two subfamilies were highly responsive to heat stress and ABA treatment, in both leaves and roots. In summary, the results from this study provided a comprehensive view of C. songoricaLEA genes and the potential applications of these genes for the improvement of crop tolerance to abiotic stresses. [ABSTRACT FROM AUTHOR]

• We use difference-in-differences estimators suitable for heterogeneous effects. • The study finds no evidence that DRC's logging concessions increase or decrease deforestation and forest degradation. • The study finds no evidence that logging concessions reduce deforestation in high deforestation pressure areas. The Congo Basin is home to the second largest tropical humid forest in the world and an important participant in climate change mitigation. While the Democratic Republic of Congo (DRC) boasts the largest proportion of Congo basin forests, it is also the main contributor to forest loss. Industrial logging concession is a forest management model that is likely to expand in the DRC in the coming years as the Government recently announced the probable end of the 2002 moratorium on logging concessions. It is thus crucial to understand whether this model can further the DRC's deforestation reduction efforts. The literature suggests that the effect of titling forestland on avoided deforestation and forest degradation is generally limited, unless environmental restrictions are enforced. The DRC's 2002 forest code introduced a new logging concession model that includes requirements to submit and implement Forest Management Plans (FMP) and increase benefit-sharing with communities. Our paper evaluates the impact of this new model of concession contracts signed in 2011 and 2014 on deforestation and forest degradation. We use remotely sensed data series to estimate difference-in-difference models. We ensured baseline parallel trends in deforestation and similarity in baseline characteristics between control and treated units. We find no evidence that deforestation and forest degradation is significantly different between logging concessions and control areas, even for concessions that had their FMP accepted. Our study highlights the need for evaluating the impact of FMPs once they are fully implemented, the importance to improve degradation outcome variables and to conduct complementary studies analyzing impact pathways. [ABSTRACT FROM AUTHOR]

Landslide, lahars and lava flow will remain a major concern for many people, until governments and scientists have fully mastered these sometimes devastating phenomena occurring in a vicinity of the volcanoes and mountains. Knowing some of their properties is therefore a challenge for scientists around the world, with a view to define and guarantee harmonious and sustainable living spaces. The association of the DOWNFLOW and FLOWGO methods and the use of the remote sensing and field data, integration and destruction indices have allowed in this work to map the directions of flows and the areas at risk of landslides, together with the thermorheological parameters of any lava. The combination of all these results produces a synthetic map of the level of risk on a mountain and shows where the surrounding populations and their activities can exist peacefully and sustainably. This study has made it possible to forecast the multi-hazard risk assessment on a mountain combining methods that are generally used separately. The originality of the method lies in the fact that it can be used with most GISs (Geographic Information Systems), making attempts to forecast risks less costly when the data used are free. [ABSTRACT FROM AUTHOR]

Melilotus is an important genus of legume plants and an herbage with excellent nitrogen fixation; it can tolerate extreme environmental conditions and possesses important medicinal value. However, there is limited genetic information about the genus; thus, we analysed four chloroplast loci (rbcL, matK, psbA-trnH and trnL-F) and one nuclear region (ITS) to determine the genetic diversity of 199 accessions from 18 Melilotus species. The rbcL and matK sequences were highly conserved, whereas the trnL-F and ITS sequences contained variable and parsimony-informative sites. In our analyses of the single and combined regions, we calculated the pairwise distance, haplotype and nucleotide diversity and gaps and then constructed phylogenetic trees to assess the genetic diversity, and our results revealed significant variations among the different accessions. The genetic distance values were between zero and nine, and based on the combined regions, the highest frequency value was approximately four. Melilotus showed high haplotype and nucleotide diversity, particularly in the ITS sequences, with values of 0.86 and 0.0087, respectively. The single ITS sequence, psbA-trnH, and the combined matK+rbcL+trnL-F (MRT) and matK+rbcL+psbA-trnH+trnL-F+ITS (MRPTI) regions showed interspecific variation in the gap analysis. Phylogenetic trees calculated using ITS, psbA-trnH and MRPTI sequences indicated distinct genetic relationship in 18 species, and these species could be divided into two groups. By determining the genetic diversity of plants, we can evaluate the genetic relationships among species and accessions, providing a basis for preserving and utilizing the genetic resources of Melilotus. [ABSTRACT FROM AUTHOR]

• In this work we study the stability of 10 embankments on some main roads in central Africa. • We find that, in general, these embankments are not stable. • To improve this situation, we propose to plaster, slice or cut some of these slopes with angles lower than 10°. Analysis of slope stability has been a challenge for researchers and engineers since the 17th century with Hooke. This paper used geometric, kinematic and seismic methods to analyse the stability of selected slopes cut into the gneiss of important roads connecting Central Africa countries, subjected to particle and block falls. The selected slopes were also studied to verify the factor on which engineers base their design. The results show that the angles are sharp and that the selected slopes are unstable, made in gneiss with a diptic state and a particle acceleration of 1.57 km/s2, confirming that the slopes are sensitive to falling particles. This study also shows that, in a moderately active seismic zone in Central Africa, slopes cut into fractured gneiss are generally unstable and engineers must also consider FS. To make these slopes more stable, they can be plastered, trenched or cut at angles <10°. [ABSTRACT FROM AUTHOR]

In the framework of an R&D program on the ALICE dimuon trigger system, the spatial resolution of Resistive Plate Chambers working in streamer mode has been investigated during beam tests. Preliminary studies with cosmic rays had evidenced a dependence of the charge profile to the high voltage and to the gas mixture. However, the spatial resolution measured from the beam tests is only slightly varying with the high voltage and is close to the expected value w/√ of 12 where w is the strip pitch, for strips of 1 and 2 cm width. The probability to fire a strip as a function of the distance from the strip to the particle impact has been measured for various high voltages. A simple parametrisation of this probability has been achieved. This allows to predict, under various working condition, the cluster size distributions for RPCs with different strip widths and also to account for the effect of the particle incident angle. [Copyright &y& Elsevier]