22 results on '"Bok-Ghee Han"'
Search Results
2. Development of SNP-based human identification system.
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Jae-Jung Kim, Bok-Ghee Han, Hae-In Lee, Han-Wook Yoo, and Jong-Keuk Lee
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GENETIC polymorphisms , *NUCLEOTIDES , *FORENSIC genetics , *DNA , *HUMAN genome - Abstract
Single nucleotide polymorphisms (SNPs) appeal to the forensic DNA community because of their abundance in the human genome, low mutation rate, small amplicon size, and feasibility of high-throughput genotyping technologies. In an initial screening, we identified six SNP markers of sex determination by resequencing the amelogenin genes and the zinc finger protein genes located on the sex chromosomes. Furthermore, for use in human identification, we selected 30 highly polymorphic autosomal SNP markers from among a human population and examined the potential utility of these SNP markers for human identification. The combined mean match probability of 30 SNP markers was 4.83 × 10−13. Using genotyping data from 8,842 unrelated Korean individuals, we also found that discrimination power increased 10-fold for the addition of every five SNP markers in human identification. In this study, we demonstrated that SNP markers are very useful for sex determination and human identification, even in a very homogeneous population. [ABSTRACT FROM AUTHOR]
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- 2010
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3. Genome-based exome sequencing analysis identifies GYG1, DIS3L and DDRGK1 are associated with myocardial infarction in Koreans.
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JI-YOUNG LEE, SANGHOON MOON, YUN KYOUNG KIM, SANG-HAK LEE, BOK-SOO LEE, MIN-YOUNG PARK, JEONG EUY PARK, YANGSOO JANG, and BOK-GHEE HAN
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MYOCARDIAL infarction , *NUCLEOTIDE sequencing , *DISEASE susceptibility , *EXOMES , *CORONARY disease , *GENETICS - Abstract
Myocardial infarction (MI) is a complex disease caused by combination of genetic and environmental factors. Although genome-wide association studies (GWAS) identified more than 46 risk loci which are associated with coronary artery disease and MI, most of the genetic variability in MI still remains undefined. Here, we screened the susceptibility loci for MI using exome sequencing and validated candidate variants in replication sets. We identified that three genes (GYG1, DIS3L and DDRGK1) were associated with MI at the discovery and replication stages. Further research will be required to determine the functional association of these genes with MI risk, and these associations have to be confirmed in other ethnic populations. [ABSTRACT FROM AUTHOR]
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- 2017
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4. MicroRNA-650 in a copy number-variable region regulates the production of interleukin 6 in human osteosarcoma cells.
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JUN HO YUN, SANGHOON MOON, HEUN-SIK LEE, MI YEONG HWANG, YEON-JUNG KIM, HO-YEONG YU, YOUNGSOO KIM, BOK-GHEE HAN, BONG-JO KIM, and JEONG-MIN KIM
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MICRORNA genetics , *DNA copy number variations , *INTERLEUKIN-6 , *OSTEOSARCOMA , *GENE expression - Abstract
Copy number variation is a well-known genetic variation. microRNAs (miRNAs/miRs) are non-coding RNAs that mediate gene expression by regulating target mRNAs. In the present study, copy number deletions encompassing miRNA coding regions were investigated to determine the association between the deletion of miRNA and its phenotypic effects. A total of 38 human miRNAs in copy number variants were identified and miR-650, which is functional in the human osteosarcoma MG-63 cell line, was selected. Overexpression of miR-650 decreased the expression of inhibitor of growth family member 4 (ING4) in the MG-63 cells and increased interleukin (IL)6 transcription, as well as IL6 secretion in IL1B-stimulated cells. Furthermore, miR-650 downregulated the amount of nuclear factor of κ light polypeptide gene enhancer in B cells inhibitor α and increased the transcriptional activity of nuclear factor (NF)κB. Downregulation of ING4 also increased the production of IL6, similar to miR-650 over-expression. Taken together, these data indicate that miR-650 plays a significant role in the production of IL6 by regulating ING4 expression and NFκB signaling in IL1B-stimulated MG-63 osteosarcoma cells. [ABSTRACT FROM AUTHOR]
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- 2015
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5. A genome-wide association study of copy-number variation identifies putative loci associated with osteoarthritis in Koreans.
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Sanghoon Moon, Bhumsuk Keam, Mi Yeong Hwang, Young Lee, Suyeon Park, Ji Hee Oh, Yeon-Jung Kim, Heun-Sik Lee, Nam Hee Kim, Young Jin Kim, Dong-Hyun Kim, Bok-Ghee Han, Bong-Jo Kim, and Juyoung Lee
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OSTEOARTHRITIS , *ARTHRITIS , *KOREANS , *GENOTYPES , *GENETICS , *DISEASES - Abstract
Background: OA is a complex disease caused by environmental and genetic risk factors. The purpose of this study is to identify candidate copy number variations (CNVs) associated with OA. Methods: We performed a genome-wide association study of CNV to identify potential loci that confer susceptibility to or protection from OA. CNV genotyping was conducted using NimbleGen HD2 3 x 720K comparative hybridization array and included samples from 371 OA patients and 467 healthy controls. The putative CNV regions identified were confirmed with a TaqMan assay. Results: We identified six genomic regions associated with OA encompassing CNV loci. None of six loci had previously been reported in genome-wide association studies with OA, although a genetic analysis suggested that they have functional effects. The protein product of a candidate risk gene for obesity, TNKS, targets Wnt inhibition, and this gene was significantly associated with hand and knee OA. Copy number deletion on TNKS was associated with a 1.37-fold decreased risk for OA. In addition, CA10, which shows a strong association with osteoporosis, was also significant in our study. Copy number deletion on this gene was associated with a 1.69-fold decreased risk for OA. Conclusion: We identified several CNV loci that may contribute to OA susceptibility in Koreans. Further functional investigations of these genes are warranted to fully characterize their putative association. [ABSTRACT FROM AUTHOR]
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- 2015
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6. Genome-Wide Association Study Identifies Two Novel Loci with Sex-Specific Effects for Type 2 Diabetes Mellitus and Glycemic Traits in a Korean Population.
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Min Jin Go, Joo-Yeon Hwang, Tae-Joon Park, Young Jin Kim, Ji Hee Oh, Yeon-Jung Kim, Bok-Ghee Han, and Bong-Jo Kim
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GENETIC epidemiology , *TYPE 2 diabetes , *GLYCEMIC index , *KOREANS , *COMPLEMENTATION (Genetics) , *REGRESSION analysis ,SEX differences (Biology) - Abstract
Background: Until recently, genome-wide association study (GWAS)-based findings have provided a substantial genetic contribution to type 2 diabetes mellitus (T2DM) or related glycemic traits. However, identification of allelic heterogeneity and population- specific genetic variants under consideration of potential confounding factors will be very valuable for clinical applicability. To identify novel susceptibility loci for T2DM and glycemic traits, we performed a two-stage genetic association study in a Korean population. Methods: We performed a logistic analysis for T2DM, and the first discovery GWAS was analyzed for 1,042 cases and 2,943 controls recruited from a population-based cohort (KARE, n= 8,842). The second stage, de novo replication analysis, was performed in 1,216 cases and 1,352 controls selected from an independent population-based cohort (Health 2, n = 8,500). A multiple linear regression analysis for glycemic traits was further performed in a total of 14,232 nondiabetic individuals consisting of 7,696 GWAS and 6,536 replication study participants. A meta-analysis was performed on the combined results using effect size and standard errors estimated for stage 1 and 2, respectively. Results: A combined meta-analysis for T2DM identified two new (rsl 1065756 and rs2074356) loci reaching genome-wide significance in CCDC63 and C12orf51 on the 12q24 region. In addition, these variants were significantly associated with fasting plasma glucose and homeostasis model assessment of (β-cell function. Interestingly two independent single nucleotide polymorphisms were associated with sex-specific stratification in this study. Conclusion: Our study showed a strong association between T2DM and glycemic traits. We further observed that two novel loci with multiple diverse effects were highly specific to males. Taken together, these findings may provide additional insights into the clinical assessment or subclassification of disease risk in a Korean population. [ABSTRACT FROM AUTHOR]
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- 2014
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7. Genome-wide copy number variation study reveals KCNIP1 as a modulator of insulin secretion.
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Heun-Sik Lee, Moon, Sanghoon, Yun, Jun Ho, MeeHee Lee, Hwang, Mi Yeong, Young-Jin Kim, Bok-Ghee Han, Jeong-Min Kim, and Bong-Jo Kim
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GENOMES , *DNA copy number variations , *INSULIN , *ETIOLOGY of diseases , *GENETIC markers , *COMPARATIVE genomic hybridization - Abstract
Copy number variations (CNVs) have emerged as another important genetic marker in addition to SNP for understanding etiology of complex diseases. In light of this, we performed a genome-wide CNV study to identify type 2 diabetes (T2D)-associated CNV using an array comparative genomic hybridization from 3180 subjects for T2D cases (n = 863) and controls (n = 2,317). Thus, five CNV regions having a p-value threshold ≤ 0.05 were identified and evaluated by validation with quantitative PCR and comparison with previously reported CNV regions in the Database of Genomic Variants. Furthermore, we performed a functional experiment to assess the biological significance of a gene encompassing a CNV region. The inhibition of KCNIP1 led to increased insulin secretion in a glucose-dependent manner, but had no effect on insulin gene transcription as well as cell apoptosis. Taken together, these data indicate that KCNIP1 from CNV study might function as a T2D-susceptibility gene whose dysregulation alters insulin production. [ABSTRACT FROM AUTHOR]
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- 2014
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8. Identification of a genetic variant at 2q12.1 associated with blood pressure in East-Asians by genome-wide scan including gene-environment interactions.
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Yun Kyoung Kim, Youngdoe Kim, Mi Yeong Hwang, Shimokawa, Kazuro, Sungho Won, Kato, Norihiro, Tabara, Yasuharu, Yokota, Mitsuhiro, Bok-Ghee Han, Jong Ho Lee, and Bong-Jo Kim
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EAST Asians , *GENETIC research , *GENOMICS , *BLOOD pressure , *HEALTH - Abstract
Background Genome-wide association studies have identified many genetic loci associated with blood pressure (BP). Genetic effects on BP can be altered by environmental exposures via multiple biological pathways. Especially, obesity is one of important environmental risk factors that can have considerable effect on BP and it may interact with genetic factors. Given that, we aimed to test whether genetic factors and obesity may jointly influence BP. Methods We performed meta-analyses of genome-wide association data for systolic blood pressure (SBP) and diastolic blood pressure (DBP) that included analyses of interaction between single nucleotide polymorphisms (SNPs) and the obesity-related anthropometric measures, body mass index (BMI), height, weight, and waist/hip ratio (WHR) in East-Asians (n = 12,030). Results We identified that rs13390641 on 2q12.1 demonstrated significant association with SBP when the interaction between SNPs and BMI was considered (P < 5 × 10 -8). The gene located nearest to rs13390641, TMEM182, encodes transmembrane protein 182. In stratified analyses, the effect of rs13390641 on BP was much stronger in obese individuals (BMI ≥ 30) than non-obese individuals and the effect of BMI on BP was strongest in individuals with the homozygous A allele of rs13390641. Conclusions Our analyses that included interactions between SNPs and environmental factors identified a genetic variant associated with BP that was overlooked in standard analyses in which only genetic factors were included. This result also revealed a potential mechanism that integrates genetic factors and obesity related traits in the development of high BP. [ABSTRACT FROM AUTHOR]
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- 2014
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9. Human transcriptome analysis of acute responses to glucose ingestion reveals the role of leukocytes in hyperglycemia-induced inflammation.
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Hyung Jin Choi, Hye Sun Yun, Hyo-Jeong Ban, Yeonjung Kim, Hye-Young Nam, Eun-Jung Hong, So-Young Jung, Seung Eun Jung, Jae-Pil Jeon, and Bok-Ghee Han
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Glucose ingestion-induced hyperglycemia has been known to induce inflammation, which is related to the pathogenesis of diabetic complications. To examine acute gene expression responses to physiological oral glucose ingestion in human circulating leukocytes, we conducted a microarray study of human circulating leukocytes sampled before, 1 h after, and 2 h after glucose ingestion in community-based participants without previous histories of diabetes (n = 60). Ingestion of 75 g glucose successfully induced acute hyperglycemia (glucose concentration 91.6 ± 5.3 mg/dl for fasting and 180.7 ± 48.5 mg/dl for 1 h after glucose ingestion). Oral glucose ingestion significantly increased the expressions of 23 genes and decreased the expressions of 13 genes [false discovery rate (FDR) P value <0.05]. These genes are significantly involved in immunity by way of natural killer cell-mediated immunity, granulocyte-mediated immunity, and the cytokine-mediated signaling pathway (FDR P value <0.05). The present study demonstrated 36 genes that showed acute gene expression change in human leukocytes within 1 h after glucose ingestion, suggesting that leukocytes participate in the inflammatory process induced by acute hyperglycemia. We believe that these results will provide some basic insight into the role of leukocytes in hyperglycemia-induced inflammation and the pathogenesis of diabetic complications. [ABSTRACT FROM AUTHOR]
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- 2012
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10. A Genome-Wide Association Study of Gestational Diabetes Mellitus in Korean Women.
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Soo Heon Kwak, Sung-Hoon Kim, Young Min Cho, Min Jin Go, Yoon Shin Cho, Sung Hee Choi, Min Kyong Moon, Hye Seung Jung, Hyoung Doo Shin, Hyun Min Kang, Cho, Nam H., In Kyu Lee, Seong Yeon Kim, Bok-Ghee Han, Jang, Hak C., and Kyong Soo Park
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GENETICS , *DIABETES , *GENOMES , *ENDOCRINE diseases , *TYPE 2 diabetes - Abstract
Knowledge regarding the genetic risk loci for gestational diabetes mellitus (GDM) is still limited. In this study, we performed a two-stage genome-wide association analysis in Korean women. In the stage 1 genome scan, 468 women with GDM and 1,242 nondiabetic control women were compared using 2.19 million genotyped or imputed markers. We selected 11 loci for further genotyping in stage 2 samples of 931 case and 783 control subjects. The joint effect of stage 1 plus stage 2 studies was analyzed by meta-analysis. We also investigated the effect of known type 2 diabetes variants in GDM. Two loci known to be associated with type 2 diabetes had a genome-wide significant association with GDM in the joint analysis. rs7754840, a variant in CDKAL1, had the strongest association with GDM (odds ratio 1.518; P = 6.65 x 10-16). A variant near MTNR1B, rs10830962, was also significantly associated with the risk of GDM (1.454; P = 2.49 x 10-13). We found that there is an excess of association between known type 2 diabetes variants and GDM above what is expected under the null hypothesis. In conclusion, we have confirmed that genetic variants in CDKAL1 and near MTNR1B are strongly associated with GDM in Korean women. There seems to be a shared genetic basis between GDM and type 2 diabetes. [ABSTRACT FROM AUTHOR]
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- 2012
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11. Genetic Susceptibility on CagA-Interacting Molecules and Gene-Environment Interaction with Phytoestrogens: A Putative Risk Factor for Gastric Cancer.
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Jae Jeong Yang, Cho, Lisa Y., Kwang-Pil Ko, Aesun Shin, Seung Hyun Ma, Bo Youl Choi, Dong Soo Han, Kyu Sang Song, Yong Sung Kim, Jong-Young Lee, Bok Ghee Han, Soung-Hoon Chang, Hai-Rim Shin, Daehee Kang, Keun-Young Yoo, and Park, Sue K.
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GENOTYPE-environment interaction , *GASTROINTESTINAL cancer , *PHYTOESTROGENS , *GENES , *CANCER risk factors , *GENETIC toxicology , *META-analysis , *SMOKING - Abstract
Objectives: To evaluate whether genes that encode CagA-interacting molecules (SRC, PTPN11, CRK, CRKL, CSK, c-MET and GRB2) are associated with gastric cancer risk and whether an interaction between these genes and phytoestrogens modify gastric cancer risk. Methods: In the discovery phase, 137 candidate SNPs in seven genes were analyzed in 76 incident gastric cancer cases and 322 matched controls from the Korean Multi-Center Cancer Cohort. Five significant SNPs in three genes (SRC, c-MET and CRK) were re-evaluated in 386 cases and 348 controls in the extension phase. Odds ratios (ORs) for gastric cancer risk were estimated adjusted for age, smoking, H. pylori seropositivity and CagA strain positivity. Summarized ORs in the total study population (462 cases and 670 controls) were presented using pooled- and meta-analysis. Plasma concentrations of phytoestrogens (genistein, daidzein, equol and enterolactone) were measured using the time-resolved fluoroimmunoassay. Results: SRC rs6122566, rs6124914, c-MET rs41739, and CRK rs7208768 showed significant genetic effects for gastric cancer in both the pooled and meta-analysis without heterogeneity (pooled OR = 3.96 [95% CI 2.05-7.65], 1.24 [95% CI = 1.01-1.53], 1.19 [95% CI = 1.01-1.41], and 1.37 [95% CI = 1.15-1.62], respectively; meta OR = 4.59 [95% CI 2.74-7.70], 1.36 [95% CI = 1.09- 1.70], 1.20 [95% CI = 1.00-1.44], and 1.32 [95% CI = 1.10-1.57], respectively). Risk allele of CRK rs7208768 had a significantly increased risk for gastric cancer at low phytoestrogen levels (p interaction<0.05). Conclusions: Our findings suggest that SRC, c-MET and CRK play a key role in gastric carcinogenesis by modulating CagA signal transductions and interaction between CRK gene and phytoestrogens modify gastric cancer risk. [ABSTRACT FROM AUTHOR]
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- 2012
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12. A Network-Based Approach to Prioritize Results from Genome-Wide Association Studies.
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Akula, Nirmala, Baranova, Ancha, Seto, Donald, Solka, Jeffrey, Nalls, Michael A., Singleton, Andrew, Ferrucci, Luigi, Tanaka, Toshiko, Bandinelli, Stefania, Yoon Shin Cho, Young Jin Kim, Jong-Young Lee, Bok-Ghee Han, and McMahon, Francis J.
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GENOMES , *PROTEIN-protein interactions , *ZINC metabolism , *PHOSPHATES , *GENE expression , *CELLULAR signal transduction , *INTERLEUKINS , *CROHN'S disease - Abstract
Genome-wide association studies (GWAS) are a valuable approach to understanding the genetic basis of complex traits. One of the challenges of GWAS is the translation of genetic association results into biological hypotheses suitable for further investigation in the laboratory. To address this challenge, we introduce Network Interface Miner for Multigenic Interactions (NIMMI), a network-based method that combines GWAS data with human protein-protein interaction data (PPI). NIMMI builds biological networks weighted by connectivity, which is estimated by use of a modification of the Google PageRank algorithm. These weights are then combined with genetic association p-values derived from GWAS, producing what we call 'trait prioritized sub-networks.' As a proof of principle, NIMMI was tested on three GWAS datasets previously analyzed for height, a classical polygenic trait. Despite differences in sample size and ancestry, NIMMI captured 95% of the known height associated genes within the top 20% of ranked sub-networks, far better than what could be achieved by a single-locus approach. The top 2% of NIMMI height-prioritized sub-networks were significantly enriched for genes involved in transcription, signal transduction, transport, and gene expression, as well as nucleic acid, phosphate, protein, and zinc metabolism. All of these sub-networks were ranked near the top across all three height GWAS datasets we tested. We also tested NIMMI on a categorical phenotype, Crohn's disease. NIMMI prioritized sub-networks involved in B- and T-cell receptor, chemokine, interleukin, and other pathways consistent with the known autoimmune nature of Crohn's disease. NIMMI is a simple, user-friendly, open-source software tool that efficiently combines genetic association data with biological networks, translating GWAS findings into biological hypotheses. [ABSTRACT FROM AUTHOR]
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- 2011
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13. A genome-wide association analysis reveals 1p31 and 2p13.3 as susceptibility loci for Kawasaki disease.
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Jae-Jung Kim, Young Mi Hong, Saejung Sohn, Gi Young Jang, Kee-Soo Ha, Sin Weon Yun, Myung Ki Han, Kyung-Yil Lee, Min Seob Song, Hyoung Doo Lee, Dong Soo Kim, Jong-Eun Lee, Eun-Soon Shin, Ji-Hyun Jang, Yeon-Su Lee, Sook-Young Kim, Jong-Young Lee, Bok-Ghee Han, Jer-Yuarn Wu, and Kwi-Joo Kim
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MUCOCUTANEOUS lymph node syndrome , *GENOMES , *VASCULITIS , *CORONARY disease , *COMMUNICABLE diseases in children - Abstract
Kawasaki disease (KD) is an acute self-limited vasculitis of infants and children that manifests as fever and signs of mucocutaneous inflammation. Coronary artery aneurysms develop in approximately 15-25% of untreated children. Although the etiology of KD is largely unknown, epidemiologic data suggest the importance of genetic factors in the susceptibility to KD. In order to identify genetic variants that influence KD susceptibility, we performed a genome-wide association study (GWAS) using Affymetrix SNP array 6.0 in 186 Korean KD patients and 600 healthy controls; 18 and 26 genomic regions with one or more sequence variants were associated with KD and KD with coronary artery lesions (CALs), respectively ( p < 1 × 10). Of these, one locus on chromosome 1p31 (rs527409) was replicated in 266 children with KD and 600 normal controls (odds ratio [OR] = 2.90, 95% confidence interval [CI] = 1.85-4.54, P = 1.46 × 10); and a PELI1 locus on chromosome 2p13.3 (rs7604693) was replicated in 86 KD patients with CALs and 600 controls (OR = 2.70, 95% CI = 1.77-4.12, P = 2.00 × 10). These results implicate a locus in the 1p31 region and the PELI1 gene locus in the 2p13.3 region as susceptibility loci for KD and CALs, respectively. [ABSTRACT FROM AUTHOR]
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- 2011
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14. Carriage of the V279F Null Allele within the Gene Encoding Lp-PLA2 Is Protective from Coronary Artery Disease in South Korean Males.
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Yangsoo Jang, Waterworth, Dawn, Jong-Eun Lee, Kijoung Song, Sujin Kim, Hyo-Soo Kim, Kyung Woo Park, Hyun-Jai Cho, Il-Young Oh, Jeong Euy Park, Bok-Soo Lee, Hyo Jeong Ku, Dong-Jik Shin, Jong Ho Lee, Sun Ha Jee, Bok-Ghee Han, Hye-Yoon Jang, Eun-Young Cho, Vallance, Patrick, and Whittaker2, John
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LIPOPROTEINS , *PHOSPHOLIPASES , *BODY mass index , *DIABETES , *SMOKING , *GLUCOSE - Abstract
Background: The Asia-specific PLA2G7 994G-T transversion leads to V279F substitution within the lipoprotein-associated phospholipase-A2 (Lp-PLA2) and to absence of enzyme activity in plasma. This variant offers a unique natural experiment to assess the role of Lp-PLA2 in the pathogenesis of coronary artery disease (CAD) in humans. Given conflicting results from mostly small studies, a large two-stage case-control study was warranted. Methodology/Principal Findings: PLA2G7 V279F genotypes were initially compared in 2890 male cases diagnosed with CAD before age 60 with 3128 male controls without CAD at age 50 and above and subsequently in a second independent male dataset of 877 CAD cases and 1230 controls. In the first dataset, the prevalence of the 279F null allele was 11.5% in cases and 12.8% in controls. After adjustment for age, body mass index, diabetes, smoking, glucose and lipid levels, the OR (95% CI) for CAD for this allele was 0.80 (0.66-0.97, p = 0.02). The results were very similar in the second dataset, despite lower power, with an allele frequency of 11.2% in cases and 12.5% in controls, leading to a combined OR of 0.80 (0.69-0.92), p = 0.002. The magnitude and direction of this genetic effect were fully consistent with large epidemiological studies on plasma Lp-PLA2 activity and CAD risk. Conclusions: Natural deficiency in Lp-PLA2 activity due to carriage of PLA2G7 279F allele protects from CAD in Korean men. These results provide evidence for a causal relationship between Lp-PLA2 and CAD, and support pharmacological inhibition of this enzyme as an innovative way to prevent CAD. [ABSTRACT FROM AUTHOR]
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- 2011
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15. Joint Identification of Multiple Genetic Variants via Elastic-Net Variable Selection in a Genome-Wide Association Analysis.
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Seoae Cho, Kyunga Kim, Young Jin Kim, Jong-Keuk Lee, Yoon Shin Cho, Jong-Young Lee, Bok-Ghee Han, Heebal Kim, Jurg Ott, and Taesung Park
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GENOMICS , *GENETIC research , *STATISTICAL bootstrapping , *REPLICATION (Experimental design) , *KOREANS , *HEALTH - Abstract
Unraveling the genetic background of common complex traits is a major goal in modern genetics. In recent years, genome-wide association (GWA) studies have been conducted with large-scale data sets of genetic variants. Most of those studies have relied on single-marker approaches that identify single genetic factors individually and can be limited in considering fully the joint effects of multiple genetic factors on complex traits. Joint identification of multiple genetic factors would be more powerful and would provide better prediction on complex traits since it utilizes combined information across variants. Here we propose a multi-stage approach for GWA analysis: (1) prescreening, (2) joint identification of putative SNPs based on elastic-net variable selection, and (3) empirical replication using bootstrap samples. Our approach enables an efficient joint search for genetic associations in GWA analysis. The suggested empirical replication method can be beneficial in GWA studies because one can avoid a costly, independent replication study while eliminating false-positive associations and focusing on a smaller number of replicable variants. We applied the proposed approach to a GWA analysis, and jointly identified 129 genetic variants having an association with adult height in a Korean population. [ABSTRACT FROM AUTHOR]
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- 2010
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16. Associations of Variants in CHRNA5/A3/B4 Gene Cluster with Smoking Behaviors in a Korean Population.
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Ming D. Li, Dankyu Yoon, Jong-Young Lee, Bok-Ghee Han, Niu, Tianhua, Payne, Thomas J., Ma, Jennie Z., and Park, Taesung
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GENES , *SMOKING , *KOREANS , *NICOTINE addiction , *GENETIC polymorphisms , *SMOKING cessation , *ETIOLOGY of diseases , *PHENOTYPES ,HEALTH of cigarette smokers - Abstract
Multiple genome-wide and targeted association studies reveal a significant association of variants in the CHRNA5-CHRNA3- CHRNB4 (CHRNA5/A3/B4) gene cluster on chromosome 15 with nicotine dependence. The subjects examined in most of these studies had a European origin. However, considering the distinct linkage disequilibrium patterns in European and other ethnic populations, it would be of tremendous interest to determine whether such associations could be replicated in populations of other ethnicities, such as Asians. In this study, we performed comprehensive association and interaction analyses for 32 singlenucleotide polymorphisms (SNPs) in CHRNA5/A3/B4 with smoking initiation (SI), smoking quantity (SQ), and smoking cessation (SC) in a Korean sample (N = 8,842). We found nominally significant associations of 7 SNPs with at least one smoking-related phenotype in the total sample (SI: P = 0.015∼0.023; SQ: P = 0.008∼0.028; SC: P = 0.018,0.047) and the male sample (SI: P = 0.001∼0.023; SQ: P = 0.001,0.046; SC: P = 0.01). A spectrum of haplotypes formed by three consecutive SNPs located between rs16969948 in CHRNA5 and rs6495316 in the intergenic region downstream from the 59 end of CHRNB4 was associated with these three smoking-related phenotypes in both the total and the male sample. Notably, associations of these variants and haplotypes with SC appear to be much weaker than those with SI and SQ. In addition, we performed an interaction analysis of SNPs within the cluster using the generalized multifactor dimensionality reduction method and found a significant interaction of SNPs rs7163730 in LOC123688, rs6495308 in CHRNA3, and rs7166158, rs8043123, and rs11072793 in the intergenic region downstream from the 59 end of CHRNB4 to be influencing SI in the male sample. Considering that fewer than 5% of the female participants were smokers, we did not perform any analysis on female subjects specifically. Together, our detected associations of variants in the CHRNA5/A3/B4 cluster with SI, SQ, and SC in the Korean smoker samples provide strong evidence for the contribution of this cluster to the etiology of SI, ND, and SC in this Asian population. [ABSTRACT FROM AUTHOR]
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- 2010
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17. Copy number variation at leptin receptor genelocus associated with metabolic traits and the riskof type 2 diabetes mellitus.
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Jae-Pil Jeon, Sung-Mi Shim, Hye-Young Nam, Gil-Mi Ryu, Eun-Jung Hong, Hyung-Lae Kim, and Bok- Ghee Han
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LEPTIN , *GENES , *TYPE 2 diabetes , *METABOLISM , *GENETIC mutation - Abstract
Background: Recent efforts have been made to link complex human traits and disease susceptibility to DNA copy numbers. The leptin receptor (LEPR) has been implicated in obesity and diabetes. Mutations and genetic variations of LEPR gene have been discovered in rodents and humans. However, the association of DNA copy number variations at the LEPR gene locus with human complex diseases has not been reported. In an attempt to study DNA copy number variations associated with metabolic traits and type 2 diabetes mellitus (T2DM), we targeted the LEPR gene locus in DNA copy number analyses. Results: We identified DNA copy number variations at the LEPR gene locus among a Korean population using genome-wide SNP chip data, and then quantified copy numbers of the E2 DNA sequence in the first two exons overlapped between LEPR and LEPROT genes by the quantitative multiplex PCR of short fluorescent fragment (QMPSF) method. Among the non-diabetic subjects (n = 1,067), lower E2 DNA copy numbers were associated with higher fasting glucose levels in men (p = 1.24 × 10-7) and women (p = 9.45 × 10-5), as well as higher total cholesterol levels in men (p = 9.96 × 10-7). In addition, the significant association between lower E2 DNA copy numbers and lower level of postprandial 2hr insulin was evident only in non-diabetic women, whereas some obesity-related phenotypes and total cholesterol level exhibited significant associations only in non-diabetic men. Logistic regression analysis indicated that lower E2 DNA copy numbers were associated with T2DM (odds ratio, 1.92; 95% CI, 1.26∼2.96; p < 0.003) in our nested case-control study. Interestingly, the E2 DNA copy number exhibited a negative correlation with LEPR gene expression, but a positive correlation with LEPROT gene expression. Conclusions: This work suggests that a structural variation at the LEPR gene locus is functionally associated with complex metabolic traits and the risk of T2DM. [ABSTRACT FROM AUTHOR]
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- 2010
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18. Association of a RUNX2 Promoter Polymorphism with Bone Mineral Density in Postmenopausal Korean Women.
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Hee-Jung Lee, Jung-Min Koh, Joo-Yeon Hwang, Kang-Yell Choi, Seung Hun Lee, Eui Kyun Park, Tae-Ho Kim, Bok Ghee Han, Ghi Su Kim, Shin-Yoon Kim, and Jong-Young Lee
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GENETIC polymorphisms , *BONE diseases , *POSTMENOPAUSE , *BIOCHEMISTRY , *VITAMIN D deficiency , *TRANSCRIPTION factors - Abstract
Osteoporosis is characterized by impaired osteoblastogenesis. Bone mineral density (BMD) is a major determinant of bone strength. RUNX2 is an osteoblast-specific transcription factor involved in osteoblast differentiation and ossification. To determine whether RUNX2 is associated with BMD in an ethnically distinct population, we investigated SNPs within the two RUNX2 promoters (P1 and P2) using the Illuminar GoldenGate system in 729 postmenopausal Korean women. Subjects bearing the minor homozygote genotype ( CC) at the RUNX2 −1025 T > C SNP (rs7771980) located in P2 showed a significant association with reduced lumbar spine BMD ( p = 0.02) and BMDs at proximal femur sites (trochanter, p = 0.05; total femur, p = 0.04) compared with subjects carrying the major homozygote genotype ( TT) or the heterozygote genotype ( TC), respectively. These results present an interesting genotype association complementary to the previously reported association of BMD with the RUNX2 −1025 T > C P2 SNP in Spanish and Australian cohorts. Therefore, we suggest that the RUNX2 P2 polymorphism ( −1025 T > C) may be a useful genetic marker for bone metabolism and may play an important role in BMD in postmenopausal Korean women. [ABSTRACT FROM AUTHOR]
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- 2009
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19. A large-scale genome-wide association study of Asian populations uncovers genetic factors influencing eight quantitative traits.
- Author
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Yoon Shin Cho, Min Jin Go, Young Jin Kim, Jee Yeon Heo, Ji Hee Oh, Hyo-Jeong Ban, Dankyu Yoon, Mi Hee Lee, Dong-Joon Kim, Miey Park, Seung-Hun Cha, Jun-Woo Kim, Bok-Ghee Han, Haesook Min, Younjhin Ahn, Man Suk Park, Hye Ree Han, Hye-Yoon Jang, Eun Young Cho, and Jong-Eun Lee
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GENOMICS , *GENOMES , *CHROMOSOMES , *GENE mapping , *GENETIC research - Abstract
To identify genetic factors influencing quantitative traits of biomedical importance, we conducted a genome-wide association study in 8,842 samples from population-based cohorts recruited in Korea. For height and body mass index, most variants detected overlapped those reported in European samples. For the other traits examined, replication of promising GWAS signals in 7,861 independent Korean samples identified six previously unknown loci. For pulse rate, signals reaching genome-wide significance mapped to chromosomes 1q32 (rs12731740, P = 2.9 × 10−9) and 6q22 (rs12110693, P = 1.6 × 10−9), with the latter ∼400 kb from the coding sequence of GJA1. For systolic blood pressure, the most compelling association involved chromosome 12q21 and variants near the ATP2B1 gene (rs17249754, P = 1.3 × 10−7). For waist-hip ratio, variants on chromosome 12q24 (rs2074356, P = 7.8 × 10−12) showed convincing associations, although no regional transcript has strong biological candidacy. Finally, we identified two loci influencing bone mineral density at multiple sites. On chromosome 7q31, rs7776725 (within the FAM3C gene) was associated with bone density at the radius (P = 1.0 × 10−11), tibia (P = 1.6 × 10−6) and heel (P = 1.9 × 10−10). On chromosome 7p14, rs1721400 (mapping close to SFRP4, a frizzled protein gene) showed consistent associations at the same three sites (P = 2.2 × 10−3, P = 1.4 × 10−7 and P = 6.0 × 10−4, respectively). This large-scale GWA analysis of well-characterized Korean population-based samples highlights previously unknown biological pathways. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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20. Gene-Based Single Nucleotide Polymorphisms and Linkage Disequilibrium Patterns of 29 Asthma Candidate Genes in the Chromosome 5q31–33 Region in Koreans.
- Author
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Ha-Jung Ryu, Ho-Youl Jung, Jung-Sun Park, Gil-Mi Ryu, Jee Yeon Heo, Jae-Jung Kim, Song-Mean Moon, Hung-Tae Kim, Jong-Young Lee, Insong Koh, Jun-Woo Kim, Jae Kyun Rho, Bok-Ghee Han, Hyungtae Kim, Choon-Sik Park, Bermseok Oh, Chan Park, Jong-Keuk Lee, and Kuchan Kimm
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ASTHMA , *GENETIC polymorphisms , *CHROMOSOMES , *NUCLEOTIDES , *CYTOKINES , *IMMUNE response - Abstract
Background and Methods: Numerous genetic studies have mapped asthma susceptibility genes to a region on chromosome 5q31–33 in several populations. This region contains a cluster of cytokines and other immune-related genes important in immune response. In the present study, to determine the genetic variations and patterns of linkage disequilibrium (LD), we resequenced all the exons and promoter regions of the 29 asthma candidate genes in the chromosome 5q31–33 region. Results: We identified a total of 314 genetic variants, including 289 single nucleotide polymorphisms (SNPs), 22 insertion/deletion polymorphisms and 3 microsatellites. Standardized variance data for allele frequency revealed substantial differences in SNP allele frequencies among different ethnic groups. Interestingly, significant ethnic differences were observed mainly in intron SNPs. LD block analysis using 174 common SNPs with a frequency of >10% disclosed strong LD within most candidate genes. No significant LD was observed across genes, except for one LD block (CD14-IK block). Gene-based haplotype analyses showed that 1–5 haplotype-tagging SNPs may be used to define the six or fewer common haplotypes with a frequency of >5%, regardless of the number of SNPs. Conclusion: Overall, our results provide useful information for the identification of immune-mediated disease genes in the chromosome 5q31–33 region, as well as valuable evidence for gene-based haplotype analysis in disease association studies. Copyright © 2006 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]
- Published
- 2006
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21. Large-scale identification and characterization of genetic variants in asthma candidate genes.
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Jae-Jung Kim, Hyun-Hee Kim, Joo-Hyun Park, Ha-Jung Ryu, JuYoung Kim, Songmean Moon, Haeok Gu, Hung-Tae Kim, Jong-Young Lee, Bok-Ghee Han, Chan Park, Kuchan Kimm, Choon-Sik Park, Jong-Keuk Lee, and Bermseok Oh
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ASTHMA , *GENES , *GENETICS , *AIRWAY (Anatomy) , *EXONS (Genetics) , *GENETIC polymorphisms , *DISEASES - Abstract
Asthma is a chronic inflammatory disorder of the airways, and a number of genetic loci are associated with the disease. Candidate gene association studies have been regarded as effective tools to study complex traits. Knowledge of the sequence variation and structure of the candidate genes is required for association studies. Thus, we investigated the genetic variants of 32 asthma candidate genes selected by colocalization of positional and functional candidate genes. We screened all exons and promoter regions of those genes using 12 healthy individuals and 12 asthma patients and identified a total of 418 single nucleotide polymorphisms (SNPs), including 270 known SNPs and 148 novel SNPs. Levels of nucleotide diversity varied from gene to gene (0.72×10−4–14.53×10−4), but the average nucleotide diversity between coding SNPs (cSNPs) and noncoding SNPs was roughly equivalent (4.63×10−4 vs 4.69×10−4). However, nucleotide diversity of cSNPs was strongly correlated to codon degeneracy. Nucleotide diversity was much higher at fourfold degenerate sites than at nondegenerate sites (9.42×10−4 vs 3.14×10−4). Gene-based haplotype analysis of asthma-associated genes in this study revealed that common haplotypes (frequency >5%) represented 90.5% of chromosomes, and they could be uniquely identified with five or fewer haplotype-tagging SNPs per gene. Therefore, our results may have important implications for the selection of asthma candidate genes and SNP markers for comprehensive association studies using large sample populations. [ABSTRACT FROM AUTHOR]
- Published
- 2005
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22. Novel Promoter Polymorphism in RUNX2 Is Associated with Serum Triglyceride Level.
- Author
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Hyoung Doo Shin, Jae-Pil Jeon, Byung Lae Park, Joon Seol Bae, Hye-Young Nam, Sung-Mi Shim, Kyong Soo Park, and and Bok-Ghee Han
- Abstract
Much research evidence supports the hypothesis that chronic, low-grade inflammation related to innate immunity may play an important role in the pathophysiology of type 2 diabetes mellitus (T2DM). Runt-related transcription factor 2 (RUNX2; MIM# 600211) acts as a scaffold that controls the integration, organization, and assembly of nucleic acids. To examine whether the novel promoter variant in RUNX2 is associated with the risk of T2DM and related phenotypes, RUNX2-742G > T was genotyped in 378 T2DM patients and 382 normal controls recruited in the Korean T2DM Study. Statistical analysis revealed that RUNX2-742G > T was associated with serum triglyceride level (TG) in nondiabetic controls, although it was not associated with the risk of T2DM. Individuals who carry T/T, T/G, and G/G genotypes had the highest (2.061 ± 020), intermediate (2.01 ± 0.19), and the lowest (1.97 + 0.18) levels of log [TG (mmol/l)] (P = 0.007), respectively. Our data on this important variant of orkuO suggest that lipid metabolism might be affected by genetic polymorphisms in the promoter region. [ABSTRACT FROM AUTHOR]
- Published
- 2008
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