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Background: Invasive alien species represent a growing threat for natural systems, economy and human health. Active surveillance and responses that readily suppress newly established colonies are effective actions to mitigate the noxious consequences of biological invasions. However, when an exotic species establishes a viable population in a new area, predicting its potential spread is the most effective way to implement adequate control actions. Emerging invasive species, despite monitoring efforts, are poorly known in terms of behaviour and capacity to adapt to the new invaded range. Therefore, tools that provide information on their spread by maximising the available data, are critical. Methods: We apply three different approaches to model the potential distribution of an emerging invasive mosquito, Aedes koreicus, in Northeast Italy: 1) an automatic statistical approach based on information theory, 2) a statistical approach integrated with prior knowledge, and 3) a GIS physiology-based approach. Each approach possessed benefits and limitations, and the required ecological information increases on a scale from 1 to 3. We validated the model outputs using the only other known invaded area in Europe. Finally, we applied a road network analysis to the suitability surface with the highest prediction power to highlight those areas with the highest likelihood of invasion. Results: The GIS physiological-based model had the highest prediction power. It showed that localities currently occupied by Aedes koreicus represent only a small fraction of the potentially suitable area. Furthermore, the modelled niche included areas as high as 1500 m a.s.l., only partially overlapping with Aedes albopictus distribution. Conclusions: The simulated spread indicated that all of the suitable portion of the study area is at risk of invasion in a relatively short period of time if no control policies are implemented. Stochastic events may further boost the invasion process, whereas competition with Aedes albopictus may limit it. According to our analysis, some of the major cities in the study area may have already been invaded. Further monitoring is needed to confirm this finding. The developed models and maps represent valuable tools to inform policies aimed at eradicating or mitigating Aedes koreicus invasion in Northeast Italy and Central Europe. [ABSTRACT FROM AUTHOR]

• 39 delusional parasitosis (DP) cases, reported in 20-yr. in Italy, are described. • Most of the DP sufferers were females aged over 50. • Seven cases of psychological contagion within the same family were recorded. • The "Digital pics sign" is described as a modern derivative of the "Matchbox sign". • Cases of harmful behaviours or even suicidal thoughts are described. Delusional parasitosis (DP) is psychiatric disorder characterized by the unshakeable belief of being infested by endo– or ectoparasites, without any evidence of infestations. Hence, DP sufferers often consult medical entomologists or dermatologists, rather than seeking help from a mental health practitioner. Here we present 39 cases of suspected DP occurred in twenty years in two Italian public medical entomology laboratories, to highlight their common features and peculiarities, based on the interviews and material brought to our laboratories. Our results confirmed that DP affects both sexes, but is more frequent in females over the age of 50 years. Cases of psychological contagion, mostly affecting elderly people, were reported within seven family units. Some rare or unique features of DP sufferers involved: the fixed belief to be infested by endoparasites, olfactory hallucinations of unpleasant smell, self-harm to get rid of the imaginary parasite and suicidal thoughts. We suggest the term "Digital pics sign" to describe a modern derivative of the "Matchbox sign", where the DP sufferers showed pictures and videos, in person or sent by email, as proofs of being infested. Medical entomologists, being the most frequently contacted professionals by DP sufferers, may be crucial to raise awareness about this psychological disorder and to encourage a multidisciplinary approach amongst medical professionals and pest control operators. Graphical abstract [Display omitted]. [ABSTRACT FROM AUTHOR]

Background: Northeastern Italy is a hotspot for several tick-borne pathogens, transmitted to animals and humans mainly by Ixodes ricinus. Here we compare the results of molecular monitoring of ticks and zoonotic TBPs over a six-year period, with the monitoring of red foxes (Vulpes vulpes) in an endemic area. Results: In the period 2011–2016, 2,578 ticks were collected in 38 sites of 20 municipalities of Belluno Province. Individual adults (264), pooled larvae (n = 330) and nymphs (n = 1984) were screened for tick-borne encephalitis virus, Borrelia burgdorferi (s.l.), Rickettsia spp., Babesia spp., Anaplasma phagocytophilum and “Candidatus Neoehrlichia mikurensis” by specific SYBR green real-time PCR assays and sequencing. The spleens of 97 foxes, culled in the period 2015–2017 during sport hunting or population control programs, were also screened. Overall, nine different pathogens were found in I. ricinus nymph and adult ticks: Rickettsia helvetica (3.69%); R. monacensis (0.49%); four species of the B. burgdorferi (s.l.) complex [B. afzelii (1.51%); B. burgdorferi (s.s.) (1.25%); B. garinii (0.18%); and B. valaisiana (0.18%)]; A. phagocytophilum (3.29%); “Candidatus N. mikurensis” (1.73%); and Babesia venatorum (0.04%). Larvae were collected and screened in the first year only and two pools (0.6%) were positive for R. helvetica. Tick-borne encephalitis virus was not found in ticks although human cases do occur in the area. The rate of infection in ticks varied widely according to tick developmental stage, site and year of collection. As expected, adults were the most infected, with 27.6% harboring at least one pathogen compared to 7.3% of nymphs. Pathogens with a minimum infection rate above 1% were recorded every year. None of the pathogens found in ticks were detectable in the foxes, 52 (54%) of which were instead positive for Babesia cf. microti (also referred to as Babesia microti-like, “Theileria annae”, “Babesia annae” and “Babesia vulpes”). Conclusions: The results show that foxes cannot be used as sentinel animals to monitor tick-borne pathogens in the specific epidemiological context of northeastern Italy. The high prevalence of Babesia cf. microti in foxes and its absence in ticks strongly suggests that I. ricinus is not the vector of this pathogen. [ABSTRACT FROM AUTHOR]