42 results on '"Chen, Ying-Xu"'
Search Results
2. Electrochemical biosensor based on Se-doped MWCNTs-graphene and Y-shaped DNA-aided target-triggered amplification strategy.
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Wang, Yi-Han, Chen, Ying-Xu, Wu, Xu, and Huang, Ke-Jing
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GRAPHENE , *ELECTROCHEMICAL sensors , *MULTIWALLED carbon nanotubes , *GENE amplification , *QUADRUPLEX nucleic acids - Abstract
Graphical abstract Highlights • Se doping MWCNTs-graphene hybrids are prepared and used for supporting substrate. • Y-shaped DNA-aided target recycling signal amplification and hemin/G-quadruplex is used for signal amplification. • The biosensor used for PDGF-BB detection shows linear range of 0.1 pM-10 nM and detection limit of 27 fM. Abstract A highly sensitive electrochemical biosensor for detection of platelet-derived growth factor-BB (PDGF-BB) is developed by using Se-doped multi-walled carbon nanotubes (MWCNTs)-graphene hybrids as electrode supporting substrate, hemin/G-quadruplex as trace labels and Y-shaped DNA-aided target recycling as signal magnifier. The aptamer-containing hairpin probes were first immobilized on the electrode. When target PDGF-BB was added, the aptamer binded PDGF-BB to trigger catalytic assembly of two other hairpins to form many G-quadruplex Y-junction DNA structures, which released PDGF-BB to again bind the intact aptamer to initiate another assembly cycle. G-quadruplex/hemin complexes were produced when hemin was added to generate substantially amplified current output. The developed assay showed a linear range toward PDGF-BB from 0.1 pM to 10 nM with a detection limit of 27 fM (S/N = 3). The method showed excellent specificity and repeatability, and could be expediently applied for sensitive detection of other molecules by simply changing the aptamers. [ABSTRACT FROM AUTHOR]
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- 2018
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3. A sandwich-type electrochemical biosensing platform for microRNA-21 detection using carbon sphere-MoS2 and catalyzed hairpin assembly for signal amplification.
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Chen, Ying-Xu, Wu, Xu, and Huang, Ke-Jing
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MICRORNA , *SPHERES , *GOLD nanoparticles , *ELECTRODE efficiency , *HORSERADISH - Abstract
In this work, a sandwich-type electrochemical biosensing platform is developed based on carbon sphere-MoS 2 (CS-MoS 2 ) and target recycling amplification of catalyzed hairpin assembly (CHA) strategy for ultrasensitive determination of microRNA (miRNA). The CS-MoS 2 is prepared and used as sensing substrate, which shows high specific surface area, enhanced stability and good dispersibility. The capture probe is first modified on Au nanoparticles/CS-MoS 2 modified glassy carbon electrode through Au-S band, and hairpin DNA1 (H1) then reacts with it and unfolds its loop domain by hybridizing with target miRNA-21. The opened H1 subsequently assembles with biotin labeled hairpin DNA 2 (H2) to displace the target. The liberated miRNA-21 is free and backs to the original solution to proceed next circulation, resulting in the generation of large amounts of H1–H2 duplex. Massive signal indicators horseradish peroxidase are then immobilized on electrode, and catalyze hydrogenperoxide + hydroquinone (H 2 O 2 + HQ) system to produce a strong electrochemical response. The proposed assay benefits from advantages of sandwich-type structure, favorable electrochemical properties of CS-MoS 2 and CHA target recycling amplification strategy. Therefore, it exhibits a wide linear relation between 1 × 10 −16 and 1 × 10 −10 M with a low detection limit of 1.6 × 10 −17 M for miRNA-21 detection. [ABSTRACT FROM AUTHOR]
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- 2018
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4. Tetrahedral DNA probe coupling with hybridization chain reaction for competitive thrombin aptasensor.
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Chen, Ying-Xu, Huang, Ke-Jing, He, Liu-Liu, and Wang, Yi-Han
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ELECTRON-transfer catalysis , *GRAPHENE oxide , *DOPING agents (Chemistry) , *THROMBIN , *TETRAHEDRAL molecules - Abstract
A novel competitive aptasensor for thrombin detection is developed by using a tetrahedral DNA (T-DNA) probe and hybridization chain reaction (HCR) signal amplification. Sulfur and nitrogen co-doped reduced graphene oxide (SN-rGO) is firstly prepared by a simple reflux method and used for supporting substrate of biosensor. Then, T-DNA probe is modified on the electrode by Au-S bond and a competition is happened between target thrombin and the complementary DNA (cDNA) of aptamer. The aptamer binding to thrombin forms an aptamer-target conjugate and make the cDNA remained, and subsequently hybridizes with the vertical domain of T-DNA. Finally, the cDNAs trigger HCR, which results in a great current response by the catalysis of horseradish peroxidase to the hydrogen peroxide + hydroquinone system. For thrombin detection, the proposed biosensor shows a wide linearity range of 10 –13 –10 −8 M and a low detection limit of 11.6 fM (S/N = 3), which is hopeful to apply in biotechnology and clinical diagnosis. [ABSTRACT FROM AUTHOR]
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- 2018
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5. Recent advances in signal amplification strategy based on oligonucleotide and nanomaterials for microRNA detection-a review.
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Chen, Ying-Xu, Huang, Ke-Jing, and Niu, Ke-Xin
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MICRORNA , *GENE amplification , *OLIGONUCLEOTIDES , *NANOSTRUCTURED materials , *NEUROLOGICAL disorders - Abstract
MicroRNAs (MiRNAs) play multiple crucial regulating roles in cell which can regulate one third of protein-coding genes. MiRNAs participate in the developmental and physiological processes of human body, while their aberrant adjustment will be more likely to trigger diseases such as cancers, kidney disease, central nervous system diseases, cardiovascular diseases, diabetes, viral infections and so on. What's worse, for the detection of miRNAs, their small size, high sequence similarity, low abundance and difficult extraction from cells impose great challenges in the analysis. Hence, it's necessary to fabricate accurate and sensitive biosensing platform for miRNAs detection. Up to now, researchers have developed many signal-amplification strategies for miRNAs detection, including hybridization chain reaction, nuclease amplification, rolling circle amplification, catalyzed hairpin assembly amplification and nanomaterials based amplification. These methods are typical, feasible and frequently used. In this review, we retrospect recent advances in signal amplification strategies for detecting miRNAs and point out the pros and cons of them. Furthermore, further prospects and promising developments of the signal-amplification strategies for detecting miRNAs are proposed. [ABSTRACT FROM AUTHOR]
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- 2018
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6. An electrochemical microRNA sensing platform based on tungsten diselenide nanosheets and competitive RNA–RNA hybridization.
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Chen, Ying-Xu, Zhang, Wen-Jing, Huang, Ke-Jing, Zheng, Mingbo, and Mao, Ya-Cen
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ELECTROCHEMICAL sensors , *MICRORNA , *TUNGSTEN selenide , *NUCLEIC acid hybridization , *MEDICAL research - Abstract
In this work, we report an ultrasensitive electrochemical biosensor for microRNA-21 (miRNA-21) detection by using a competitive RNA–RNA hybridization configuration. A biotinylated miRNA of the self-same sequence with the target miRNA is mixed with the samples, and allowed competition with the target miRNA for a thiolated RNA probe immobilized onto a tungsten diselenide (WSe2) nanosheet modified electrode. Thereafter the current response is obtained by forming the hybridized biotinylated miRNA with streptavidin–horseradish peroxidase (HRP) conjugates to catalyze the H2O2 + hydroquinone (HQ) system. Benefiting from the high specific surface area of WSe2 nanosheets, the competitive hybridization configuration and the signal amplification of the H2O2 + HQ detection system, the proposed assay exhibits a wide linear range of 0.0001–100 pM towards target miRNA with a detection limit of 0.06 fM (S/N = 3), and shows excellent discrimination ability for base-mismatched miRNA sequences. Therefore, the designed platform has promising prospects for the detection of miRNA in biomedical research and early clinical diagnosis. [ABSTRACT FROM AUTHOR]
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- 2017
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7. Ultrasensitive electrochemical sensing platform based on graphene wrapping SnO2 nanocorals and autonomous cascade DNA duplication strategy.
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Chen, Ying-Xu, Huang, Ke-Jing, Lin, Feng, and Fang, Lin-Xia
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ELECTROCHEMICAL sensors , *STANNIC oxide , *GRAPHENE , *DEOXYRIBOZYMES , *POLYMERIZATION , *ENDONUCLEASES - Abstract
In this work, a sensitive, universal and reusable electrochemical biosensor based on stannic oxide nanocorals-graphene hybrids (SnO 2 NCs-Gr) is developed for target DNA detection by using two kinds of DNA enzymes for signal amplification through an autonomous cascade DNA duplication strategy. A hairpin probe is designed composing of a projecting part at the 3′-end as identification sequence for target, a recognition site for nicking endonuclease, and an 18-carbon shim to stop polymerization process. The designed DNA duplication–incision–replacement process is handled by KF polymerase and endonuclease, then combining with gold nanoparticles as signal carrier for further signal amplification. In the detection system, the electrochemical–chemical–chemical procedure, which uses ferrocene methanol, tris(2-carboxyethyl)phosphine and l -ascorbic acid 2-phosphate as oxidoreduction neurogen, deoxidizer and zymolyte, separately, is applied to amplify detection signal. Benefiting from the multiple signal amplification mechanism, the proposed sensor reveals a good linear connection between the peak current and logarithm of analyte concentration in range of 0.0001–1 × 10 –11 mol L −1 with a detection limit of 1.25 × 10 –17 mol L −1 (S/N=3). This assay also opens one promising strategy for ultrasensitive determination of other biological molecules for bioanalysis and biomedicine diagnostics. [ABSTRACT FROM AUTHOR]
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- 2017
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8. Fatty liver index correlates with non-alcoholic fatty liver disease, but not with newly diagnosed coronary artery atherosclerotic disease in Chinese patients.
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Zhao-Yan Jiang, Chen-Ying Xu, Xian-Xing Chang, Wei-Wei Li, Lu-Ying Sun, Xiao-Bo Yang, Li-Fen Yu, Jiang, Zhao-Yan, Xu, Chen-Ying, Chang, Xian-Xing, Li, Wei-Wei, Sun, Lu-Ying, Yang, Xiao-Bo, and Yu, Li-Fen
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FATTY liver , *FATTY degeneration , *LIVER diseases , *CORONARY arteries , *CARDIOVASCULAR diseases - Abstract
Background: Fatty liver index (FLI) was recently established to predict non-alcoholic fatty liver disease (NAFLD) in general population, which is known to be associated with coronary artery atherosclerotic disease (CAD).This study aims to investigate whether FLI correlates with NAFLD and with newly diagnosed CAD in a special Chinese population who underwent coronary angiography.Methods: Patients with CAD (n = 231) and without CAD (n = 482) as confirmed by coronary angiography were included. Among them, 574 patients underwent B-ultrosonography were divided into NAFLD group (n = 209) and non-NAFLD group (n = 365). Correlation between FLI and NAFLD was analyzed using pearson's correlation. The associations between FLI and NAFLD as well as CAD were assessed using logistic regression. The predictive accuracy of FLI for NAFLD was evaluated using receiver operating characteristics (ROC) curve analysis.Results: FLI was significantly higher in NAFLD group (37.10 ± 1.95) than in non-NAFLD group (17.70 ± 1.04), P < 0.01. FLI correlated with NAFLD (r = 0.372, P < 0.001). The algorithm for FLI had a ROC-AUC of 0.721 (95% CI: 0.678-0.764) in the prediction of NAFLD. Logistic regression analysis showed that FLI was associated with NAFLD (adjusted OR = 1.038, 95% CI: 1.029-1.047, P < 0.01). The proportion of patients with CAD did not differ among the groups of FLI ≤ 30 (32.3%), 30-60 (31.0%), and ≥60 (35.3%). No significant association was found between FLI and CAD (adjusted OR = 0.992, 95% CI: 0.981-1.003 in men and OR = 0.987, 95% CI: 0.963-1.012 in women, P > 0.05).Conclusions: FLI showed good correlation with NAFLD in patients who underwent coronary angiography, but not with newly diagnosed CAD. This might be underestimated because some patients in non-CAD group may have other underlying cardiovascular diseases. [ABSTRACT FROM AUTHOR]- Published
- 2013
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9. Optimization of struvite crystallization protocol for pretreating the swine wastewater and its impact on subsequent anaerobic biodegradation of pollutants
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Zhang, Dong-mei, Chen, Ying-xu, Jilani, Ghulam, Wu, Wei-xiang, Liu, Wen-li, and Han, Zhi-ying
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CRYSTALLIZATION , *WASTEWATER treatment , *ANAEROBIC digestion , *BIODEGRADATION , *POLLUTANTS , *CHEMICAL reduction , *PRECIPITATION (Chemistry) , *HYDROGEN-ion concentration - Abstract
Abstract: Higher contents of and SS in wastewater hamper the anaerobic digestion; necessitating its pretreatment to reduce them. This study reveals optimization of struvite/MAP precipitation protocol followed by anaerobic digestion of pretreated swine wastewater for pollutants removal. Levels of different treatments: stirring speeds, 400 and 160rpm; pH values, 9.0, 9.5, 10.0, 10.5, 11.0 and 11.5; and P:Mg:N ratios, 1:1:1.2, 1:1:1.7, 1:1:2.2, 1:1:2.7, 1:1:4.0 and 1:1:5.0 were evaluated for MAP crystallization. Among various combinations, protocol comprising of initial 10min stirring at 400rpm followed by 160rpm for 30min, pH 10.0, and P:Mg:N ratio 1:1:1.2 rendered the best removal efficiency for , , COD, TC and TOC. Subsequent anaerobic biodegradation revealed superiority of MAP supernatant over raw swine wastewater for methane yield and , , COD, TC and TOC removals. It suggests that struvite precipitation as pretreatment to anaerobic digestion is highly effective and advantageous in wastewater treatment. [Copyright &y& Elsevier]
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- 2012
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10. Effects of Tibetan hulless barley on bloom-forming cyanobacterium (Microcystis aeruginosa) measured by different physiological and morphologic parameters
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Xiao, Xi, Chen, Ying-xu, Liang, Xin-qiang, Lou, Li-ping, and Tang, Xian-jin
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CYANOBACTERIA , *CYANOBACTERIAL blooms , *MICROCYSTIS aeruginosa , *MORPHOLOGY , *GLOBAL warming , *CYTOMETRY , *CELL membranes , *CHLOROPHYLL , *FLUORESCENCE , *ALLELOPATHY - Abstract
Abstract: The current trend of global warming is expected to stimulate the expansion of harmful cyanobacteria blooms. Previously, the occidental type of barley straw has been used to control blooms in Europe and America, but very little is known about the antialgal abilities of its oriental relative. We tested the use of Tibetan hulless barley straw – the progenitor of oriental barley – to inhibit the growth of cyanobacterium Microcystis aeruginosa. Flow cytometry allowed assessment at single-cell level, with morphologic parameters (cell volume, cell membrane integrity) and physiological parameters (in vivo Chlorophyll a fluorescence, metabolic activity) used as endpoints. The reduction of cell densities together with integrated cell membranes suggests that Tibetan barley may act as an algistatic agent. Doses from 2.0 to 8.0gL−1 of Tibetan barley straw efficiently inhibited the alga, but these doses were much higher than those of occidental barley. Such a large dosage introduced additional nutrients, which stimulated the intracellular metabolic activity and induced two physiological subpopulations in the acute term. After mid and long-term exposure, the growth inhibition effect exceeded the stimulation effect, so that the cells’ metabolic activity and Chlorophyll a fluorescence decayed, simultaneously with shrinkage in the algal cell volume. [ABSTRACT FROM AUTHOR]
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- 2010
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11. Effects of bamboo charcoal and bamboo vinegar on nitrogen conservation and heavy metals immobility during pig manure composting
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Chen, Ying-Xu, Huang, Xiang-Dong, Han, Zhi-Ying, Huang, Xiao, Hu, Bin, Shi, De-Zhi, and Wu, Wei-Xiang
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BAMBOO , *ORGANIC waste purification , *VAPORIZATION in water purification , *NITROGEN removal (Water purification) , *ORGANIC fertilizers , *COMPOSTING , *HEAVY metal toxicology - Abstract
Abstract: Composting is an effective methodology for the treatment of organic waste like pig manure (PM) before land application. However, nitrogen loss through NH3 volatilization during the thermophilic phase is one of the major disadvantages of composting. The presence of Cu and Zn in compost arising from pig feed additives is also an issue. In this study the effects of bamboo charcoal (BC) and bamboo vinegar (BV) added to composting piles on nitrogen conservation and immobility of Cu and Zn during PM composting was investigated. Total Kjeldahl nitrogen (TKN) loss and mobility of Cu and Zn decreased with increased BC addition. TKN loss and mobility of Cu and Zn in the treatment with 9% BC at the end of composting significantly decreased by 65%, 35% and 39% respectively, as compared to the control. Addition of BV further decreased TKN loss. After composting, TKN loss in the treatment with 3% BC+0.4% BV was 23% lower than that in the treatment with 9% BC. The final results indicated that by adding BC or BC+BV into PM composting is an effective method to reduce TKN loss and control the mobility of Cu and Zn. [Copyright &y& Elsevier]
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- 2010
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12. Model AVSWAT apropos of simulating non-point source pollution in Taihu lake basin
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Zhang, Qiu-Ling, Chen, Ying-Xu, Jilani, Ghulam, Shamsi, Imran Haider, and Yu, Qiao-Gang
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NONPOINT source pollution , *WATER pollution , *MATHEMATICAL models , *EUTROPHICATION , *WATERSHEDS , *WATER quality monitoring , *SENSITIVITY analysis , *LAKES - Abstract
Abstract: Accelerated eutrophication and nutrient loads in the lakes are of major concern for human health and environment. This study was undertaken for modeling the non-point source pollution of Taihu lake basin in eastern China. The SWAT model having an interface in ArcView GIS was employed. Model sensitive parameters related to hydrology and water quality were obtained by sensitivity analysis, and then calibrated and validated by comparing model predictions with field data. The GIS showed good potential for parameterization of hill-slopes, channels, and representative slope profiles for SWAT model simulations. In a monthly and daily time step, the model''s Nash-Sutcliffe coefficient (E) and the coefficient of determination (R 2) indicated that values of simulated runoff, NH4 +–N and total phosphorus were acceptably closer to the measured data. Surface water parameters especially CN, Soil-AWC and ESCO were the most sensitive and had more recognition in the model. It is concluded that runoff carrying N and P nutrients from chemical fertilizer inputs in agricultural areas is the major contributor to NPSP in the lake basin. So, decrease in excessive use of N and P fertilizers and their synergism with organic manures is recommended that would significantly reduce nutrient pollution in the lake ecosystem. [Copyright &y& Elsevier]
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- 2010
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13. Denitrification capacity of bioreactors filled with refuse at different landfill ages
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Chen, Ying-xu, Wu, Song-wei, Wu, Wei-xiang, Sun, Hua, and Ding, Ying
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DENITRIFICATION , *CHEMICAL reduction , *BIOREACTORS , *LANDFILL gases , *NITRO compounds , *NITRATES , *NITROGEN - Abstract
Abstract: The denitrification capacity of refuse at different landfill ages in bioreactor landfill system was studied. Three reactors filled with 1-year-old refuse (R1), 6-year-old refuse (R6) and 11-year-old refuse (R11), respectively, were operated in the experiment. Nitrate solution (1000mgNO3 −-NL−1) was added into each reactor. The results showed that the reactors were all able to consume nitrate. However, 1-year-old refuse in R1 had both a higher nitrate reduction rate and concentration of N2. In addition, vertical differences in nitrate removal along the depth of R1 were observed. The bottom-layer refuse and the middle-layer refuse both showed higher efficiency in nitrate depletion than the top layer. Furthermore, N2O accumulation was found in R11 with the concentration up to 19.3% of the released gas. These results suggested that 1-year-old refuse, which was partly degraded, was more suitable to use as denitrification medium. [Copyright &y& Elsevier]
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- 2009
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14. Nitrogen interception in floodwater of rice field in Taihu region of China
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LIANG, Xin-qiang, CHEN, Ying-xu, LI, Hua, TIAN, Guang-ming, ZHANG, Zhi-jian, NI, Wu-zhong, and HE, Miao-miao
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RIVERS , *NITROGEN , *MANURES - Abstract
Abstract: A field experiment located in Taihu Lake Basin of China was conducted, by application of urea or a mixture of urea with manure, to elucidate the interception of nitrogen (N) export in a typical rice field through “zero-drainage water management” combined with sound irrigation, rainfall forecasting and field drying. N concentrations in floodwater rapidly declined before the first event of field drying after three split fertilizations, and subsequently tended to return to the background level. Before the first field drying, total particulate nitrogen (TPN) was the predominant N form in floodwater of plots with no N input, dissolved inorganic nitrogen (DIN) on plots that received urea only, and dissolved organic nitrogen (DON) on plots treated with the mixture of urea and manure. Thereafter TPN became the major form. No N export was found from the rice field, but total nitrogen (TN) of 15.8 kg/hm2 was remained, mainly due to soil N sorption. The results recommended the zero-drainage water management for full-scale areas for minimizing N export. [Copyright &y& Elsevier]
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- 2007
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15. Influence of the DMPP (3,4-dimethyl pyrazole phosphate) on nitrogen transformation and leaching in multi-layer soil columns
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Yu, Qiao-Gang, Chen, Ying-Xu, Ye, Xue-Zhu, Tian, Guang-Ming, and Zhang, Zhi-Jian
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NITROGEN fertilizers , *LEACHING , *SOIL profiles , *NITRIFICATION , *GROUNDWATER , *OXIDATION , *UREA , *PYRAZOLES - Abstract
The application of nitrogen fertilizers leads to various ecological problems such as nitrate leaching. The use of nitrification inhibitors as nitrate leaching retardants is a proposal that has been suggested for inclusion in regulations in many countries. In this study, using a multi-layer soil column device, the influence of new nitrification inhibitor DMPP (3,4-dimethyl pyrazole phosphate) was studied for understanding the nitrogen vertical transformation and lowering the nitrate leaching at different soil profile depths. The results indicated that, within 60 d of experiment, the regular urea added 1.0% DMPP can effectively inhibit the ammonium oxidation in the soil, and improve the ammonium concentration in soil solution over the 20cm depths of soil profile, while decline the concentrations of nitrate and nitrite. No obvious difference was found on ammonium concentrations in soil solution collected from deep profile under 20cm depths between regular urea and the urea added 1.0% DMPP. There was also no significant difference for the nitrate, ammonium and nitrite concentrations in the soil solution under 40cm depths of soil profile with the increasing nitrogen application level, among the treatments of urea added 1.0% DMPP within 60 d. It is proposed that DMPP could be used as an effective nitrification inhibitor in some region to control ammonium oxidation and decline the ion-nitrogen leaching, minimizing the shallow groundwater pollution risk and being beneficial for the ecological environment. [Copyright &y& Elsevier]
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- 2007
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16. Transformation of heavy metal forms during sewage sludge bioleaching
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Chen, Ying-Xu, Hua, Yu-Mei, Zhang, Shao-Hui, and Tian, Guang-Ming
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SEWAGE disposal , *HEAVY metals , *SEWAGE sludge , *HYDROGEN-ion concentration - Abstract
Abstract: The transformation of heavy metal forms during sewage sludge bioleaching with elemental sulfur as substrate was investigated. The sequential selective extraction procedure was an effective way to reflect the variation in heavy metal forms. The exchangeable form of Cu, Pb and Zn after bioleaching accounted for 81.6, 40.2 and 75.8%, respectively. Cu at initial sludge mainly existed as sulfide precipitate form, most of which turned to exchangeable form during bioleaching, and was solubilized mostly by direct mechanism. The original forms of Pb and Zn were mainly carbonate precipitate and organically bound. Most of the carbonate precipitate Pb transformed into exchangeable form but a little of organically bound Pb. Pb was dissolved from the sludge due to transformation of carbonate precipitated and sulfide precipitated to exchangeable form by cooperation of both direct and indirect mechanisms. A majority of exchangeable Zn was transformed from organically bound and carbonate precipitated forms, and the bioleaching mechanism of Zn was mainly indirect. After bioleaching, the sewage sludge could be applied to land more safely because the heavy metals mainly existed in stable forms. The exchangeable Cu and Pb achieved an obvious increase at pH about 2, while exchangeable Zn showed higher percentage at pH about 3. The transformation of chemical forms for Cu had good relationship with ORP during bioleaching, but Zn was not influenced by ORP of sludge, which appeared to have high bioleaching efficiency at ORP less than 200mV. [Copyright &y& Elsevier]
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- 2005
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17. Effect of copper-tolerant rhizosphere bacteria on mobility of copper in soil and copper accumulation by Elsholtzia splendens
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Chen, Ying Xu, Wang, Yuan Peng, Lin, Qi, and Luo, Yong Ming
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FUNGUS-bacterium relationships , *RHIZOBACTERIA , *SOIL moisture , *BIOREMEDIATION - Abstract
Abstract: The role of rhizosphere bacteria in facilitating the solubility of copper (Cu) in contaminated soil and Cu accumulation in plant were studied. The bacteria strains were isolated from the rhizosphere of Elsholtzia splendens, a Cu accumulator growing on Tonglu Mountain copper mines. After the sandy soils containing 237 mg kg−1 were incubated with the bacteria strains, it was indicated that rhizosphere microbes played an important role in influencing the availability of water-soluble Cu in soils. Soils had greater concentrations of water-extractable Cu compared with axenic soils inoculated with different bacterial strains. Further evidence for bacterial facilitation of increased solubility of Cu in the soil was obtained using the antibiotic ampicillin (0.1 mg g− 1). There were 36% decreases in Cu concentration in the presence of bacterial strain MS12 and ampicillin together compared with bacterial inoculation alone. Different bacterial strains had different abilities on soil water-soluble Cu. To achieve the highest rates of plant Cu accumulation, it was necessary for bacteria to be present in the rhizosphere of E. splendens. Inoculated plants supplied with 20 μmol L− 1 CuSO4 had significantly greater concentrations of Cu in shoots and roots than uninoculated plants and bacterial strain MS2 was the most effective strain in promoting plant Cu uptake. There were 2.2-fold and 2.5-fold increases in Cu accumulation in the shoots and roots of plants inoculated with strain MS2 compared to axenic controls. Furthermore, when ampicillin and the bacterial strains were added together to the nutrient solution, the Cu concentrations in roots and shoots of ampicillin-treated plants were lower than those in inoculated plants. When ampicillin was added to the nutrient solution, Cu accumulation was inhibited by about 24–44% in shoots and 20–44% in roots. The above results provided a new insight into the phytoremediation of Cu-contaminated soil. [Copyright &y& Elsevier]
- Published
- 2005
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18. Microbial activity related to N cycling in the rhizosphere of maize stressed by heavy metals.
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Yang Ye, Chen Ying-xu, Tian Guang-ming, and Zhang Zi-jian
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PLANT-soil relationships , *PLANT roots , *NITRIFYING bacteria , *HEAVY metals , *PROKARYOTES , *FUNGUS-bacterium relationships - Abstract
A greenhouse experiment was carried out to compare differences in potential activities of ammonification, nitrification and denitrification in rhizosphere and bulk soil in a heavy-metal-stressed system. Exchangeable fractions of Cd, Cu and Cr were all higher in the rhizosphere of maize than in bulk soil. Results showed that the mineralization of N in soil was stimulated by low concentration of Cd. Addition of Cd at low levels stimulated the ammonifying and nitrifying activity in soil, while inhibitory influences were shown at high levels. Nitrifying bacteria was proved to be the most sensitive one, whilst the effect on denitrifying bacteria was very limited. Comparing Cd, Cu and Cr(VI) at 20 mg/kg soil, Cd was the most effective inhibitor of ammonification and denitrification, while Cr(VI) had the strongest inhibitory influence on nitrifying activity. Root exudates played important roles on the different exchangeable metal fractions and bacterial activities between rhizosphere and non-rhizosphere. Nitrate was the main form of mineral N in soil, as well as the main form of N absorbed by plants, but the formation and relative absorption of ammonium were promoted in response to high Cd exposure. [ABSTRACT FROM AUTHOR]
- Published
- 2005
19. Long-term operation of biofilters for biological removal of ammonia
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Chen, Ying-Xu, Yin, Jun, and Wang, Kai-Xiong
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AMMONIA , *NITROGEN compounds , *COMPOSTING , *SOIL amendments , *NITRIFICATION - Abstract
Abstract: Biological removal of ammonia was investigated using two types of packing materials, compost and sludge in laboratory-scale biofilters (8l reactor volume). The aim of this study is to investigate the potential of unit systems packed with these supports in terms of ammonia emissions treatment. Experimental tests and measurements included analysis of removal efficiency, metabolic products, and results of long-term operation. The inlet concentration of ammonia applied was 20–200mgm−3. The ammonia loading rates of 24.9–566gNH3m−3d−1 to compost biofilter (BF3) and 24.9–472gNH3m−3d−1 to sludge biofilter (BF4) were applied for 210 days, respectively. Removal efficiencies of the compost and sludge biofilters were in the range of 97–99% and 95–99%, respectively when the inlet concentration of ammonia was below 110mgm−3, and the maximum elimination capacities were 288 and 243gNH3m−3d−1, respectively. However, removal efficiency and elimination capacity of both biofilters significantly decreased as the inlet concentration increased to above 110mgm−3. By using kinetic analysis, the maximum removal rate of ammonia, Vm, and the saturation constant, Ks, were determined for both packing materials and the value of Vm for compost was found to be larger. Periodic analysis of the biofilter packing materials showed the accumulation of the nitrification product in the operation. During the experiment, the pressure drops measured were very low. The use of both packing materials requires neither nutritive aqueous solution nor buffer solution. [Copyright &y& Elsevier]
- Published
- 2005
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20. Advanced start-up of anaerobic attached film expanded bed reactor by pre-aeration of biofilm carrier
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Ye, Fen-xia, Chen, Ying-xu, and Feng, Xiao-shan
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BIOFILMS , *BIOMASS , *DEHYDROGENASES , *COENZYMES - Abstract
The start-up and performance of the anaerobic attached film expanded bed (AAFEB) reactor with pre-aeration of carrier were investigated. The carriers of the reactors had been aerated for 10 days before they were put into the AAFEB reactors. The results indicated that the reactors advance the start-up by 15 days, and maintain higher efficiency when they were subjected to organic and hydraulic loading shock, but during steady-state operation, the reactors did not show better performance than the control reactors without pre-aeration of carrier. The thicker biofilm and higher biomass concentration of the reactors with pre-aeration were observed during the start-up period, but the difference between two types of reactors tapered with the time course, and at the steady-state operation, the difference between two types of reactors on these two parameters was not obvious. Maximum specific methane or acids production rates, dehydrogenase activity and coenzyme F420 content were continuously higher than those of the control reactors. After running 30 days, filamentous bacteria dominated in the reactors with pre-aeration, whereas the cocci were predominant species in the control reactors. It was suggested that the action of the biofilm is strongly dependent on the biofilm thickness or the biomass concentration in normal circumstances, but under adverse circumstances, such as organic or hydraulic loading shock, the characteristics and activity of the anaerobic granular sludge play key roles on the reactor performance. These results clearly indicated that pre-aeration of carrier favor to enhance the start-up and performance of AAFEB reactor. [Copyright &y& Elsevier]
- Published
- 2005
- Full Text
- View/download PDF
21. Root-induced changes of lead availability in the rhizosphere of Oryza sativa L.
- Author
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Lin, Qi, Chen, Ying Xu, He, Yun Feng, and Tian, Guang Ming
- Subjects
- *
PLANT roots , *RHIZOSPHERE , *METALLURGY , *ENVIRONMENTAL impact analysis - Abstract
Geogenic, as well as anthropogenic heavy metals from distant sources, gradually increase the level of toxic metals in natural environments and these will be increasingly taken up by plants and transferred further up the food chain. To control any detrimental environmental impacts of soil contaminants, it is desirable to investigate the solubility and mobility of soil chemical contaminants under land-based agricultural systems. In this paper, a greenhouse experiment was conducted to assess the effects of rhizospheric dynamics on the bioavailability of lead. Rice (Oryza sativa L.) and Ultisol were used as the test materials. The results showed NH4OAc extractable Pb in rice rhizosphere was much higher than in bulk soil, which meant that the activation processes in the rhizosphere was significant and the amount of bioavailable Pb increased. The distribution of extractable Pb at a distance of 0–10 mm from the root compartment, however, varied and had a little depletion compared to that in the rhizosphere. The significant difference of Pb forms between the rhizosphere and bulk soil further demonstrated the root-induced changes of lead bioavailability in the rhizosphere might also be related to the various levels of Pb treatment and the simultaneity of Cd. It was observed the accumulation of soluble plus exchangeable Pb (SE-Pb) in the 500 mg kg-1 Pb treatment, and relatively stronger fixation of Pb to oxides in the 250 mg kg-1 Pb treatment. The activation of SE-Pb in rice rhizosphere was weaker in the 5 mg kg-1 Cd treatment and stronger in the 10 mg kg-1 Cd treatment than that in the treatment without Cd. The activation of lead in the rhizosphere of Oryza sativa L., suggested a potential risk of heavy metal accumulation and phytotoxicity in plants and soil ecosystem. [Copyright &y& Elsevier]
- Published
- 2004
- Full Text
- View/download PDF
22. Effect of substrate concentration on the bioleaching of heavy metals from sewage sludge.
- Author
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Chen Ying-Xu, Hua Yu-Mei, and Zhang Shao-Hui
- Subjects
- *
HEAVY metals , *BACTERIAL leaching , *POTASSIUM , *ALKALI metals , *NITROGEN , *WASTE management - Abstract
The effect of elemental sulfur concentration on bioleaching of Cu, Zn and Pb and loss of fertilizer value from sewage sludge was investigated in flasks by batch experiments. The results showed that the ultimate pH of sludges with 3-5 g/L of sulfur added was about 1.3 and the production of SO42+ had good correlation with the elemental sulfur concentration. The sensitivity of removal efficiency of metals to sulfur concentration was: Pb > Cu > Zn. The sulfur concentration except for 3 - 5 g/L had significant effect on the solubilization of Cu, Pb and Zn. The highest solubilization efficiency for sludge with 3 g/L of sulfur was 87.86% for Cu, 32.72% for Pb and 92.14% for Zn which could make the treated sludge easily meet the metal limitations for land application. The sulfur concentration of 3 g/L was enough for the solubilization of all three heavy metals. The influence of sulfur concentration on solubilization of total nitrogen and potassium from sludge was negligible. but that on solublization of total phosphorus was of great importance. The loss of nitrogen phosphorus and potassium of sludge with 3 g/L of sulfur by bioleaching was 38.2%, 52.1% and 42.8% respectively, and the sludge still remained satisfactory fenilizer value after bioteaching. [ABSTRACT FROM AUTHOR]
- Published
- 2004
23. A novel tri-primer PCR method (TP-PCR) for rapid construction of fpg gene
- Author
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Chen, Ying Xu, Liu, He, Zhang, Wen Bo, and Jin, Yong Feng
- Subjects
- *
GREEN fluorescent protein , *ENDONUCLEASES , *POLYMERASE chain reaction , *GENES - Abstract
A novel tri-primer polymerase chain reaction method (TP-PCR) was developed for the construction of a fused fpg gene, in which no endonuclease and ligase were used. Instead, two templates and three specifically designed primers were applied. Results showed that pheB and gfp genes, which encodes the catechol 2, 3-dioxygenase and the green fluorescent protein (GFP), respectively, were successfully fused into an fpg gene through the rapid TP-PCR system, indicating that TP-PCR method could be a useful tool for DNA fragment fusion in which no proper endonuclease sites were available. [Copyright &y& Elsevier]
- Published
- 2004
- Full Text
- View/download PDF
24. Irreversible sorption of pentachlorophenol to sediments: experimental observations
- Author
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Chen, Ying-Xu, Chen, Hua-Lin, Xu, Yun-Tai, and Shen, Meng-Wei
- Subjects
- *
PENTACHLOROPHENOL , *SEDIMENT transport , *ABSORPTION , *ORGANIC compounds , *CARBON - Abstract
The partitioning behavior of pentachlorophenol (PCP) in five sediments was studied using equilibrium sorption experiments and multiple cycles of sorption and desorption experiments. The results of the equilibrium sorption experiments showed that the isotherms of PCP on five sediments were linear and the partitioning coefficients (Kd) were proportional to the organic carbon content of the sediments. The average organic carbon content normalized partitioning coefficient (logKoc) of five sediments was 2.83±1.48. In multiple cycles of sorption and desorption experiments, the five sediments were found to exhibit statistically significant sorption–desorption hysteresis, and the hysteresis indices (HI) varied over a wide range (0.72–11.82). Correlations between the HI value and the percentage of lipid in the total organic matter in the sediment indicated that lipid was the main fraction to affect the hysteresis phenomenon, i.e., the higher the lipid percentage the greater the HI value. The hysteresis phenomenon was mostly caused by irreversible sorption of PCP on lipids, including entrapment by lipids, which induced the slow desorption rate from the sediment. Because of hysteresis in the sorption and desorption, the PCP ecological toxicity would be lower than expected. [Copyright &y& Elsevier]
- Published
- 2004
- Full Text
- View/download PDF
25. Phosphorus and copper leaching from dredged sediment applied on a sandy loam soil: column study
- Author
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Chen, Ying-Xu, Zhu, Guang-Wei, Tian, Guang-Ming, and Chen, Hua-Lin
- Subjects
- *
PHOSPHORUS , *COPPER , *LEACHING , *ATTENTION , *RESEARCH - Abstract
In this paper, downward movement of phosphorus and copper as dredged sediment applied on sandy loam soil was studied by column leaching experiments. Three sediment application rate, (i.e., 1, 2 and 5-cm depth of sediments) were applied to the top of the soil columns. Two and a half months leaching experiments were conducted, which include a 15-day un-watered period. Concentrations of phosphorus and copper in the leachate and the vertical distribution of Olsen-P and diethylenetriaminepentaacetic acid (DTPA) extractable Cu in the soil columns were determined. The results showed that, un-watered period could increase the downward movements of phosphorus and copper. Sediment application significantly increased Olsen-P concentration in the top 15 cm of the soil columns, but has not significantly affected that in the deeper soil layer. The 1-cm depth sediment treatment did not increase the DTPA extractable Cu concentration in the whole soil column. The 5-cm depth sediment treatment, however, significant increased the DTPA extractable Cu in the deeper soil layers. This study suggested that the application of dredged sediment laden with P and Cu on sandy loam soil might cause the significant downward movement of phosphorus and copper. [Copyright &y& Elsevier]
- Published
- 2003
- Full Text
- View/download PDF
26. Reduction of nitrate from groundwater: powder catalysts and catalytic membrane.
- Author
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Chen Ying-xu, S. F., Zhang Yan, and Liu Hong-yuan, S. F.
- Subjects
- *
CATALYSTS , *HYDROGENATION , *BIOLOGICAL membranes , *NITRATES , *AMMONIUM , *GROUNDWATER - Abstract
The reduction of nitrate contaminant in groundwater has gained renewed and intensive attention due to the environmental problems and health risks. Catalytic denetrification presents one one of the most promising approaches for the removal of nitrate from water. Catalytic nitrate reduction from water by powder catalysts and catalytic membrane in a batch reactor was studied. And the effects of the initial concentration, the amounts of catalyst, and the flux H[sub2] on the nitrate reductiion were also discussed. The results demonstrated that nitrate reduction activity and the selectivity to nitrogen gas were mainly controlled by diffusion limitations and the mass transfer of the reactants. The selectivity can improved while retaining a high catalytic activity under conrolled diffusion condition or the intensification of the mass transfer, and a good reaction condition. The total nitrogen removal efficiency reached above 80%. Moreover, catalytic membrane can create a high effective gas/liquid/solid interface, and show a good selectivity to nitrogen in comparative with the power catalyst, the selectivity to nitrogen was improved from 73.4% to 89.4%. [ABSTRACT FROM AUTHOR]
- Published
- 2003
- Full Text
- View/download PDF
27. Au nanoparticles/hollow molybdenum disulfide microcubes based biosensor for microRNA-21 detection coupled with duplex-specific nuclease and enzyme signal amplification.
- Author
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Shuai, Hong-Lei, Huang, Ke-Jing, Chen, Ying-Xu, Fang, Lin-Xia, and Jia, Meng-Pei
- Subjects
- *
GOLD nanoparticles , *MOLYBDENUM disulfide , *BIOSENSORS , *MICRORNA , *NUCLEASES , *ELECTROCHEMICAL sensors - Abstract
An ultrasensitive electrochemical biosensor for detecting microRNAs is fabricated based on hollow molybdenum disulfide (MoS 2 ) microcubes. Duplex-specific nuclease, enzyme and electrochemical–chemical–chemical redox cycling are used for signal amplification. Hollow MoS 2 microcubes constructed by ultrathin nanosheets are synthesized by a facile template-assisted strategy and used as supporting substrate. For biosensor assembling, biotinylated ssDNA capture probes are first immobilized on Au nanoparticles (AuNPs)/MoS 2 modified electrode in order to combine with streptavidin-conjugated alkaline phosphatase (SA-ALP). When capture probes hybridize with miRNAs, duplex-specific nuclease cleaves the formative duplexes. At the moment, the biotin group strips from the electrode surface and SA-ALP is incapacitated to attach onto electrode. Then, ascorbic acids induce the electrochemical–chemical–chemical redox cycling to produce electrochemical response in the presence of ferrocene methanol and tris (2-carboxyethyl) phosphine. Under optimum conditions, the proposed biosensor shows a good linear relationship between the current variation and logarithm of the microRNAs concentration ranging from 0.1 fM to 0.1 pM with a detection limit of 0.086 fM (S/N=3). Furthermore, the biosensor is successfully applied to detect target miRNA-21 in human serum samples. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
28. Layered molybdenum selenide stacking flower-like nanostructure coupled with guanine-rich DNA sequence for ultrasensitive ochratoxin A aptasensor application.
- Author
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Huang, Ke-Jing, Shuai, Hong-Lei, and Chen, Ying-Xu
- Subjects
- *
NUCLEOTIDE sequence , *MOLYBDENUM selenides , *DNA , *NUCLEIC acids , *GUANINE , *CHEMICAL detectors , *NANOSTRUCTURED materials - Abstract
Sensitive detection of ochratoxin A (OTA) plays an important role in the field of food safety control. In this study, we are reporting for the first time an electrochemical biosensor which employs the integration of MoSe 2 nanoflowers, Au nanoparticles (AuNPs), aptamer and the guanine-rich complementary DNA sequence for the sensitive detection of OTA. MoSe 2 nanoflowers are prepared by a simple hydrothermal method and used as a promising sensing platform based on its large surface area. The methylene blue is used as a tracer and a guanine-rich complementary DNA sequence is designed to bind with the unbound OTA aptamer for signal amplification. The aptamer is immobilized on the AuNPs/MoSe 2 modified electrode surface through Au–S interaction. Since OTA can competitively bind with the aptamer due to their high affinity, the presence of OTA will induce the decrease of guanine-rich DNA on the electrode surface, in turn reduces the signal of methylene blue which can specifically adsorb to the guanine units of DNA. The proposed assay shows a good linear relationship within the range of 0.0001–1 nM with a detection limit of 0.08 pM for OTA. The developed biosensor has been applied to determine OTA concentration in wine samples. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
29. Optimization of FDA–PI method using flow cytometry to measure metabolic activity of the cyanobacteria, Microcystis aeruginosa
- Author
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Xiao, Xi, Han, Zhi-ying, Chen, Ying-xu, Liang, Xin-qiang, Li, Hua, and Qian, Yi-chao
- Subjects
- *
CYANOBACTERIA , *MICROCYSTIS aeruginosa , *METABOLISM , *FLUORESCEIN , *FLOW cytometry , *ENZYME inhibitors , *ESTERASES , *HYDROLYSIS - Abstract
Abstract: A rapid toxicity test based on inhibition of esterase activity in the harmful freshwater microalgae –Microcystis aeruginosa was developed using flow cytometry. The hydrolysis rate of fluorescein diacetate (FDA) by intracellular esterase to fluorescein was used to indicate the metabolic activity of algae. Uptake of FDA was optimized at different concentrations and incubation times. Propidium iodide (PI) was utilized to assess cell membrane integrity. The optimized FDA/PI staining dosages were 10mg/L and 10μM, respectively, lower than the reported concentrations. Correspondingly, the proper incubation time was 14–21min at the optimal FDA dosage determined in this study. A new procedure based on optimized FDA/PI condition, called “whole algal culture flow cytometry with fluorescence triggering”, was developed for short-term bioassays. This new procedure, taking account of working conditions such as pH and impure cultures, is able to avoid algal cell damages in sample preparation and separate algal cells from non-algal particles by fluorescence triggering. This newly-developed procedure was then used to assess the toxicity of copper on M. aeruginosa in a short-term exposure (36h). As copper concentrations increased, it was found that the esterase activity decreased in a concentration-dependent manner with increased membrane fragments. Moreover, esterase activity was a good indicator of copper toxicity in M. aeruginosa. The EC50 value based on mean fluorescence intensity (MFI) was 123.3μg/L (95% confidence limits 101.5–146.2μg/L). Therefore, the new-developed procedure could be used for sublethal endpoints detection, and has the potential to be a rapid and cost-effective bioassay for selecting M. aeruginosa control methods or exploring the M. aeruginosa activity inhibition mechanism. [Copyright &y& Elsevier]
- Published
- 2011
- Full Text
- View/download PDF
30. An investigation of cellular distribution of manganese in hyperaccumlator plant Phytolacca acinosa Roxb. using SRXRF analysis.
- Author
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Xu, Xiang-hua, Shi, Ji-yan, Chen, Ying-xu, Xue, Sheng-guo, Wu, Bei, and Huang, Yu-ying
- Subjects
- *
HYPERACCUMULATOR plants , *EFFECT of manganese on plants , *PHYTOREMEDIATION , *PHYTOLACCA , *MANGANESE in soils , *METABOLIC detoxification , *X-ray spectroscopy , *EPIDERMIS , *BIOACCUMULATION , *LOCALIZATION theory , *SYNCHROTRON radiation - Abstract
Phytolacca acinosa Roxb. (P. acinosa) is a recently discovered manganese hyperaccumulator plant from southern China. It is a good candidate for phytoremediation of manganese(Mn) polluted soil for its high biomass and fast growth. Knowledge of the tissue localization and identification of heavy metals can provide essential information on metal toxicity and bioaccumulation mechanisms. Synchrotron radiation X-ray fluorescence spectroscopy (SRXRF) microprobe was used in this study to investigate the cellular distributions of Mn and other elements in root, stem, leaf, petiole and midrib ofP. acinosa. The highest Mn content was found in the vascular tissues of root, stem, petiole and midrib. Cortex in root played a key role in Mn absorption and Mn was limited in the vascular bundle during the process of transportation in stem. Moreover, Mn content in leaf epidermis was higher than that in mesophyll, which suggested that the sequestration of Mn in leaf epidermis might be one of the detoxification mechanisms of P. acinosa. The significance of other elemental (such as P, S, K, Ca, Fe, Zn and Cu) distribution patterns and the correlation with Mn were also discussed. [ABSTRACT FROM AUTHOR]
- Published
- 2006
- Full Text
- View/download PDF
31. Effect of organic fractions on sorption properties of organic pollutants in sediments.
- Author
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Chen Hua-lin, Zhou Jiang-min, Chen Ying-xu, and Xu Yun-tai
- Subjects
- *
POLLUTANTS , *ORGANIC compounds , *HUMIC acid , *PHENANTHRENE , *LEACHING , *OXIDES - Abstract
Humic substrates are a major fraction of sediment organic matters, and the sorption of hydrophobic organic chemicals by humic substrates influences their behavior and fate in sediment. In this paper, organic matters were divided into non-humic substrates and humic substrates. Non-humic substrates include acid leaching fraction, acid extracted fraction, and lipid; humic substrates were fractionated into Ca-binding-FA (fulvic acid), Ca-binding-HA (humic acid), oxide-FA, oxide-HA, and humin. To study the effect of organic fractions on sorption properties, sorption kinetic and equilibrium sorption experiments of phenanthrene and pentachlorophenol (PCP) in five sediments were carried out. The results showed that the contents of acid leaching fraction and oxide-binding-HA were the main fractions to affect the sorption rate constant, and for the sorption capacity of phenanthrene, humin was the major fraction, followed by oxide-binding-HA, oxide-binding-FA, and so on. While for PCP, the factors of influence on sorption capacity were mainly CEC, Ca-binding-FA, and Ca-binding-HA. [ABSTRACT FROM AUTHOR]
- Published
- 2005
32. Recent advances in nanomaterial-based electrochemical and optical sensing platforms for microRNA assays.
- Author
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Wang, Yi-Han, He, Liu-Liu, Huang, Ke-Jing, Chen, Ying-Xu, Wang, Shu-Yu, Liu, Zhen-Hua, and Li, Dan
- Subjects
- *
MICRORNA , *QUANTUM dots , *OPTICAL elements , *DEVELOPMENTAL biology - Abstract
MicroRNA (MiRNA) plays a crucial role in biological cells to enable assessment of a cancer's development stage. Increasing evidence has shown that the accurate and sensitive detection of miRNA holds the key toward correct disease diagnosis. However, some characteristics of miRNAs, such as their short chains, low concentration, and similar sequences, make it difficult to detect miRNA in biological samples. Nanomaterials usually have good optical, electronic, and mechanical properties and therefore provide new possibilities for improving the performance of miRNA assays. Many different sorts of nanomaterials, including metal nanomaterials, carbon nanomaterials, quantum dots, and transition-metal dichalcogenides, have been used to construct optical and electrochemical assays for miRNA and have shown attractive results. This review describes recent efforts in the application of nanomaterials as sensing elements in electrochemical and optical miRNA assays. The analytical figures of merit of various methods for the detection of miRNA are compared in the present article. The current capabilities, limitations, and future challenges in miRNA detection and analysis based on nanomaterials are also addressed. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
33. Ultrasensitive electrochemical sensing platform for microRNA based on tungsten oxide-graphene composites coupling with catalyzed hairpin assembly target recycling and enzyme signal amplification.
- Author
-
Shuai, Hong-Lei, Huang, Ke-Jing, Xing, Ling-Li, and Chen, Ying-Xu
- Subjects
- *
ELECTROCHEMICAL analysis , *MICRORNA , *TUNGSTEN oxides , *HYDROTHERMAL alteration , *BIOSENSORS - Abstract
An ultrasensitive electrochemical biosensor for microRNA (miRNA) is developed based on tungsten oxide-graphene composites coupling with catalyzed hairpin assembly target recycling and enzyme signal amplification. WO 3 -Gr is prepared by a simple hydrothermal method and then coupled with gold nanoparticles to act as a sensing platform. The thiol-terminated capture probe H1 is immobilized on electrode through Au–S interaction. In the presence of target miRNA, H1 opens its hairpin structure by hybridization with target miRNA. This hybridization can be displaced from the structure by another stable biotinylated hairpin DNA (H2), and target miRNA is released back to the sample solution for next cycle. Thus, a large amount of H1-H2 duplex is produced after the cyclic process. At this point, a lot of signal indicators streptavidin-conjugated alkaline phosphatase (SA-ALP) are immobilized on the electrode by the specific binding of avidin-biotin. Then, thousands of ascorbic acid, which is the enzymatic product of ALP, induces the electrochemical-chemical-chemical redox cycling to produce a strongly electrochemical response in the presence of ferrocene methanol and tris (2-carboxyethyl) phosphine. Under the optimal experimental conditions, the established biosensor can detect target miRNA down to 0.05 fM (S/N=3) with a linear range from 0.1 fM to 100 pM, and discriminate target miRNA from mismatched miRNA with a high selectivity. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
34. Improving Anammox start-up with bamboo charcoal
- Author
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Chen, Chong-jun, Huang, Xiao-xiao, Lei, Chen-xiao, Zhu, Wei-jing, Chen, Ying-xu, and Wu, Wei-xiang
- Subjects
- *
BAMBOO , *CHARCOAL , *UPFLOW anaerobic sludge blanket (UASB) reactor , *COMPARATIVE studies , *AMMONIUM , *SUSPENDED solids , *EVAPORATION (Chemistry) , *POLYMERASE chain reaction - Abstract
Abstract: Three Upflow Anaerobic Sludge Blanket (UASB) reactors were compared for Anammox enrichment using synthetic wastewater with Spherical Plastic (SP) and Bamboo Charcoal (BC) addition, and without carrier (CK). After four months of operation, the Anammox activity occurred in all reactors allowing continuous removal of ammonium and nitrite. Ammonium and nitrite removal efficiencies were all higher than 98% in steady phase with the effluent concentrations below 1mgL−1. The start-up time could be shortened from 117 to 97d in CK and SP reactor to 85d in BC amendment reactor. Quantitative PCR (q-PCR) analyses indicated a significant increase in the number of Anammox bacteria in BC amended reactor as compared with CK and SP during the entire start-up periods. The copy numbers of Anammox of 16SrRNA gene in the reactor with BC amendment could reach up to 6×109 copiesg−1 Volatile Suspended Solids, around 22.5times and 12.3times greater than that in CK and SP reactor, respectively. BC addition could accelerate the start-up of Anammox and significantly increase the Anammox bacteria number. [Copyright &y& Elsevier]
- Published
- 2012
- Full Text
- View/download PDF
35. Denitrification capacity in response to increasing nitrate loads and decreasing organic carbon contents in injected leachate of a simulated landfill reactor
- Author
-
Wu, Wei-xiang, Hao, Yong-jun, Ding, Ying, and Chen, Ying-xu
- Subjects
- *
DENITRIFICATION , *NITRATES , *CARBON compounds , *LEACHATE , *CHEMICAL reactors , *LANDFILLS , *MUNICIPAL solid waste incinerator residues - Abstract
Abstract: Ex situ nitrification followed by denitrification inside the landfill has been recommended to remove ammonia from leachate. The effects of increasing nitrate load and decreasing organic carbon content in the injected leachate on the denitrifying capacity of municipal solid waste (MSW) were investigated. Results showed that MSW possesses a high denitrification capacity. Nitrate reduction could be initiated within 48h after the first addition of nitrate. Nitrate reduction rate increased with the increasing nitrate loading concentration. When the nitrate loading concentration was increased to 850mgL−1, nitrate reduction rate reached up to 35mgL−1 h−1. Nitrite accumulation could be found after the addition of nitrate in each test. However, the maximum nitrite accumulation efficiency declined with increased nitrate load. Organic carbon played an important role in the reduction of nitrate, and both endogenous and exogenous organic materials could act as electron donors. [Copyright &y& Elsevier]
- Published
- 2009
- Full Text
- View/download PDF
36. Source separation of household waste: A case study in China
- Author
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Zhuang, Ying, Wu, Song-Wei, Wang, Yun-Long, Wu, Wei-Xiang, and Chen, Ying-Xu
- Subjects
- *
HAZARDOUS wastes , *WASTE recycling , *SEPARATION (Technology) , *WASTE products - Abstract
Abstract: A pilot program concerning source separation of household waste was launched in Hangzhou, capital city of Zhejiang province, China. Detailed investigations on the composition and properties of household waste in the experimental communities revealed that high water content and high percentage of food waste are the main limiting factors in the recovery of recyclables, especially paper from household waste, and the main contributors to the high cost and low efficiency of waste disposal. On the basis of the investigation, a novel source separation method, according to which household waste was classified as food waste, dry waste and harmful waste, was proposed and performed in four selected communities. In addition, a corresponding household waste management system that involves all stakeholders, a recovery system and a mechanical dehydration system for food waste were constituted to promote source separation activity. Performances and the questionnaire survey results showed that the active support and investment of a real estate company and a community residential committee play important roles in enhancing public participation and awareness of the importance of waste source separation. In comparison with the conventional mixed collection and transportation system of household waste, the established source separation and management system is cost-effective. It could be extended to the entire city and used by other cities in China as a source of reference. [Copyright &y& Elsevier]
- Published
- 2008
- Full Text
- View/download PDF
37. Effect of MSW source-classified collection on the emission of PCDDs/Fs and heavy metals from incineration in China
- Author
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Shi, De-Zhi, Wu, Wei-Xiang, Lu, Sheng-Yong, Chen, Tong, Huang, Hui-Liang, Chen, Ying-Xu, and Yan, Jian-Hua
- Subjects
- *
MUNICIPAL buildings , *SOLID waste , *INDUSTRIAL wastes , *HEAVY metals & the environment , *FLY ash - Abstract
Municipal solid waste (MSW) source-classified collection represents a change in MSW management in China and other developing countries. Comparative experiments were performed to evaluate the effect of a newly established MSW source-classified collection system on the emission of PCDDs/Fs (polychlorinated dibenzo-p-dioxins and dibenzofurans) and heavy metals (HMs) from a full-scale incinerator in China. As a result of presorting and dewatering, the chlorine level, heavy metal and water content were lower, but heat value was higher in the source-classified MSW (classified MSW) as compared with the conventionally mixed collected MSW (mixed MSW). The generation of PCDDs/Fs in flue gas from the classified MSW incineration was 9.28ng I-TEQ/Nm3, only 69.4% of that from the mixed MSW incineration, and the final emission of PCDDs/Fs was only 0.12ng I-TEQ/Nm3, although activated carbon injection was reduced by 20%. The level of PCDDs/Fs in fly ash from the bag filter was 0.27ng I-TEQ/g. These results indicated that the source-classified collection with pretreatment could improve the characteristics of MSW for incineration, and significantly decrease formation of PCDDs/Fs in MSW incineration. Furthermore, distributions of HMs such as Cd, Pb, Cu, Zn, Cr, As, Ni, Hg in bottom ash and fly ash were investigated to assess the need for treatment of residual ash. [Copyright &y& Elsevier]
- Published
- 2008
- Full Text
- View/download PDF
38. Municipal solid waste decomposition under oversaturated condition in comparison with leachate recirculation
- Author
-
Hao, Yong-Jun, Wu, Wei-Xiang, Wu, Song-Wei, Sun, Hua, and Chen, Ying-Xu
- Subjects
- *
INDUSTRIAL wastes , *PLANT engineering , *CENTRALIZED industrial waste treatment facilities , *BIOGAS - Abstract
Abstract: Municipal solid waste (MSW) decomposition under oversaturated condition in comparison with leachate recirculation was investigated in two simulated reactors (A and B). Reactor B was operated with leachate recirculation while in reactor A, an oversaturated condition was maintained without leachate recirculation. Results showed that MSW decomposition in reactor A was much faster than that in reactor B. The final CODcr concentration and pH value of leachates in reactor A were around 1000mgL−1 and 7.0, respectively, in comparison to 18,000mgL−1 and below 6.5 in reactor B after 420 days of incubation. Biogas production was positively related to ambient temperature. The total biogas and methane yields were 2800L and 1330L in reactor A while only 1470L and 600L in reactor B. These results indicated that the relative retardation of MSW degradation in reactor B was primarily resulted from the increased resistance of mass transfer and the accumulation of biogas caused by leachate recirculation and the resultant acid inhibition. Bioreactor landfill operated under liquid oversaturated and MSW loose condition could be a promising approach for accelerating municipal solid waste degradation. [Copyright &y& Elsevier]
- Published
- 2008
- Full Text
- View/download PDF
39. Heavy metal availability and impact on activity of soil microorganisms along a Cu/Zn contamination gradient
- Author
-
WANG, Yuan-peng, SHI, Ji-yan, LIN, Qi, CHEN, Xin-cai, and CHEN, Ying-xu
- Subjects
- *
HEAVY metals , *SOIL microbiology , *SOIL pollution , *BIOMASS - Abstract
Abstract: All the regulations that define a maximum concentration of metals in the receiving soil are based on total soil metal concentration. However, the potential toxicity of a heavy metal in the soil depends on its speciation and availability. We studied the effects of heavy metal speciation and availability on soil microorganism activities along a Cu/Zn contamination gradient. Microbial biomass and enzyme activity of soil contaminated with both Cu and Zn were investigated. The results showed that microbial biomass was negatively affected by the elevated metal levels. The microbial biomass-C (Cmic)/organic C (Corg) ratio was closely correlated to heavy metal stress. There were negative correlations between soil microbial biomass, phosphatase activity and NH4NO3 extractable heavy metals. The soil microorganism activity could be predicted using empirical models with the availability of Cu and Zn. We observed that 72% of the variation in phosphatase activity could be explained by the NH4NO3-extractable and total heavy metal concentration. By considering different monitoring approaches and different viewpoints, this set of methods applied in this study seemed sensitive to site differences and contributed to a better understanding of the effects of heavy metals on the size and activity of microorganisms in soils. The data presented demonstrate the relationship between heavy metals availability and heavy metal toxicity to soil microorganism along a contamination gradient. [Copyright &y& Elsevier]
- Published
- 2007
- Full Text
- View/download PDF
40. Trimethylamine (TMA) biofiltration and transformation in biofilters
- Author
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Ding, Ying, Shi, Ji-Yan, Wu, Wei-Xiang, Yin, Jun, and Chen, Ying-Xu
- Subjects
- *
BIOFILTRATION , *BIOREMEDIATION , *NITRIFICATION , *COMPOSTING , *SEWAGE sludge , *BIODEGRADATION , *FILTERS & filtration , *AMMONIA - Abstract
Bioremoval of trimethylamine (TMA) in two three-stage biofilters packed with compost (A) and sludge (B), respectively, was investigated. Both biofilters were operated with an influent TMA concentration of 19.2–57.2mgm−3 for 67 days. Results showed that all of the inlet TMA could be removed by both biofilters. However, removal efficiency and transformation of TMA in each section of both biofilters was different. In the Introduction section, TMA removal efficiency and maximum elimination capacity of the compost medium were greater than those of sludge medium under higher inlet TMA concentration. In comparison with biofilter A, considerably higher NH3 concentrations in effluent of all three sections in biofilter B were observed after day 19. Although, NO2 −-N concentration in each section of biofilter A was relatively lower, NO3 −-N content in each section of biofilter A increased after day 26, especially in the Materials and method section which increased remarkably due to a lesser amount of TMA and higher ammonia oxidation and nitrification in compost medium. In contrast, neither NO2 −-N nor NO3 −-N were detected in either section of biofilter B at any time throughout the course of the experiment. The cumulative results indicated that compost is more favorable for the growth of TMA-degrading and nitrifying bacteria as compared to the sludge and could be a highly suitable packing material for biodegradation and transformation of TMA. [Copyright &y& Elsevier]
- Published
- 2007
- Full Text
- View/download PDF
41. Reaction site and mechanism in the UV or visible light induced TiO_2 photodegradation of Orange G.
- Author
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Yang Shi-ying, Lou Li-ping, Wu Xiao-na, and Chen Ying-xu
- Subjects
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TITANIUM dioxide , *OXIDES , *FLUORINATION , *CHEMICAL reactions , *HALOGENATION , *PHOTOCATALYSIS , *AZO dyes , *ENVIRONMENTAL protection , *ENVIRONMENTAL sciences - Abstract
For TiO_2 heterogeneous reaction, the reaction site and the detailed mechanism are interesting and controversy topics. In this paper, effects of surface fluorination of TiO_2 on the photocatalytic degradation of an azo dye, Orange G(OG) under UV or visible light irradiation were investigated, and the possible reaction site and mechanism were elucidated. The adsorption of OG on TiO_2 was nearly inhibited by fluoride but its UV light induced photodegradation rate was greatly increased by a factor of about 2.7, which was due to the more generated free hydroxyl radicals. It supported the views that fluoride could desorb the oxidant species from surface and that the reaction sites could move to the bulk solution. In TiO_2/Vis system, the observed inhibition effects of fluorination could be interpreted by the competitive adsorption, which provided additional evidences that the visible light sensitized photodegradation of dye pollutants on the catalyst surface. [ABSTRACT FROM AUTHOR]
- Published
- 2006
42. Biosorption of copper(II) and zinc(II) from aqueous solution by Pseudomonas putida CZ1
- Author
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Chen, Xin Cai, Wang, Yuan Peng, Lin, Qi, Shi, Ji Yan, Wu, Wei Xiang, and Chen, Ying Xu
- Subjects
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BIOREMEDIATION , *HYDROGEN-ion concentration , *REACTION time , *SURFACE chemistry - Abstract
Abstract: To study Pseudomonas putida CZ1, having high tolerance to copper and zinc on the removal of toxic metals from aqueous solutions, the biosorption of Cu(II) and Zn(II) by living and nonliving P. putida CZ1 were studied as functions of reaction time, initial pH of the solution and metal concentration. It was found that the optimum pH for Zn(II) removal by living and nonliving cells was 5.0, while it was 5.0 and 4.5, respectively, for Cu(II) removal. At the optimal conditions, metal ion biosorption was increased as the initial metal concentration increased. The adsorption data with respect to both metals provide an excellent fit to the Langmuir isotherm. The binding capacity of living cells is significantly higher than that of nonliving cells at tested conditions. It demonstrated that about 40–50% of the metals were actively taken up by P. putida CZ1, with the remainder being passively bound to the bacterium. Moreover, desorption efficiency of Cu(II) and Zn(II) by living cells was 72.5 and 45.6% under 0.1M HCl and it was 95.3 and 83.8% by nonliving cells, respectively. It may be due to Cu(II) and Zn(II) uptake by the living cells enhanced by intracellular accumulation. [Copyright &y& Elsevier]
- Published
- 2005
- Full Text
- View/download PDF
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