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Metastasis suppressor 1 (MTSS1) has been reported to play important roles in suppressing cancer progression. In this study, we investigated the underlying mechanism that regulates MTSS1 expression. We showed that in breast cancer cells, lncRNA-SNHG15-induced cell invasion and proliferation was accompanied with the decreased expression of MTSS1 mRNA. Further study revealed that SNHG15 mediated MTSS1 repression through blocking its promoter activity. Mechanistically, SNHG15 complexes with DDX5 and RTF1 and interacts with the core promoter of the MTSS1 gene to interfere with RNA-Pol-II-directed transcriptional initiation. Association with DDX5 stabilizes SNHG15 while binding to RTF1 allows SNHG15 to carry RTF1 to the core promoter, where RTF1 forms a complex with PNA pl II to enhance transcriptional pausing. Our findings revealed a molecular mechanism by which SNHG15 serves as a regulator to suppresses MTSS1 transcription via interaction with the gene core promoter. [ABSTRACT FROM AUTHOR]

AbstractSustainability in a workplace is built upon the inclusion of aging workers and gender equality. Although prior tourism research has examined the impact of employees’ age and gender on customers’ behavioral responses, little effort has been made to examine the joint effect of these two characteristics. Thus, using an extended stereotype content model, this study examines the mechanisms through which tour guides’ age and gender affect customers’ WOM intentions. Across two experimental studies, we identified a significant influence of the two-way interaction of age and gender such that older (vs. younger) age decreased WOM intentions for female tour guides but not for their male counterparts. We further identified that warmth and competence differences explain the effect of age on WOM intentions for male and female tour guides differently. For female tour guides, the negative influence of age on WOM intentions was explained by lower levels of perceived warmth. In contrast, participants perceived older male tour guides as less competent than their younger counterparts, which then significantly influenced decreased WOM intentions. However, customers showed higher levels of warmth toward older tour guides, and this perceived warmth inference counteracts the perceived deficiency in competence. These findings have significant theoretical and practical implications. [ABSTRACT FROM AUTHOR]

Consider a credit portfolio with the investments in various sectors and exposed to an external stochastic environment. The portfolio loss due to defaults is of critical importance for social and economic security particularly in times of financial distress. We argue that the dependences among obligors within sectors (intradependence) and across sectors (interdependence) may coexist and influence the portfolio loss. To quantify the portfolio loss, we develop a multi-sector structural model in which a multivariate regular variation structure is employed to model the intradependence within sectors, and the interdependence across sectors is implied in the arbitrarily dependent macroeconomic factors, although, given them, obligors in different sectors are conditionally independent. We establish some sharp asymptotic formulas for the tail probability and the (tail) distortion risk measures of the portfolio loss. Our results show that the portfolio loss is mainly driven by the latent variables and the recovery rate function, and is also potentially affected by the macroeconomic factors and the intradependence within sectors. Moreover, we implement intensive numerical studies to examine the accuracy of the obtained approximations and conduct some sensitivity analysis. [ABSTRACT FROM AUTHOR]

LncRNAs are emerging as important regulators of gene expression by controlling transcription in the nucleus and by modulating mRNA translation in the cytoplasm. In this study, we reveal a novel function of lncRNA SNHG15 in mediating breast cancer cell invasion through regulating the local translation of CDH2 mRNA. We show that SNHG15 preferentially localizes at the cellular protrusions or cell leading edge and that this localization is directed by IMP1, a multifunctional protein involved in many aspects of RNA regulation. We demonstrate that SNHG15 also forms a complex with nucleolin, allowing nucleolin to be co-transported with SNHG15 to the cell protrusions, where the accumulated nucleolin is able to bind to CDH2 mRNA. Interaction with nucleolin stabilizes local CDH2 mRNA and regulates its translation, thus promoting cell invasive potential. Our findings reveal an underlying mechanism by which lncRNA could serve as a carrier to transport a protein regulator into a specific cell compartment to enhance target mRNA expression. [ABSTRACT FROM AUTHOR]

Multiple clusters of fracturing within one stage have been widely used in the unconventional reservoir production. However, the non-uniform fracture propagation under multiple clusters of fracturing has not yet fully been understood, due to the fluid flow in the wellbore being neglected in the fracturing model. In view of this, a wellbore-fracture coupled fracturing model was established to simulate fracture non-uniform propagation under multiple clusters of fracturing. In this model, the governing equation was firstly given based on the pressure continuity and flow conservation, to obtain the flow distribution in each fracture. Then, equations of fracture propagation, fracture height growth and stress shadow were combined to determine the fracture geometric parameters. Substituting fracture geometric parameters into the governing equation, the wellbore-fracture coupled fracturing model was finally solved. The proposed model was validated by a ABAQUS cohesive elements numerical by comparing the fracture parameters, respectively considering conditions of wellbore-fracture coupling and non-wellbore-fracture coupling, within a maximum deviation that did not exceed 6.14%. Using the proposed model, the parametric analyses were performed, including energy-related parameters and wellbore-related parameters. For energy-related parameters, with the increase of surface energy density of rock and the minimum principal stress, the fracture length and height decreased, while the fracture width increased to some extent. In addition, with the increase of pumping rate of fracturing fluid, the fracture length, height and width increased significantly. For wellbore-related parameters, with the decrease of diameter of perforation and perforation number as well as the increase of wellbore roughness coefficient and viscosity of fracturing fluid and cluster distance, the flow distribution in each fracture and the injection pressure became more uniform and the non-uniform propagation of each fracture was reduced, but fracture dimensions were simultaneously decreased. [ABSTRACT FROM AUTHOR]

• A special advantage on casing deformation relieved was observed for densified-multi-cluster fracturing. • An analytical model of casing deformation relieved under densified-multi-cluster fracturing was established. • Fracture-induced stresses, as casing additional loads, were integrated into calculation of casing stresses. • Analytical model was validated by numerical model, with the maximum deviation within 7.4 %. • Casing stresses were decreased with increasing of fracture cluster amounts, illustrating the mechanism proposed. The densified-multi-cluster fracturing, a technology being used to enhance shale gas production, also shows a special advantage on casing deformation relieved in WeiRong oil field, southwest China, compared to the conventional multi-cluster fracturing. In view of this, an analytical model was established to illustrate the mechanism of casing deformation relieved under densified-multi-cluster fracturing from the viewpoint of casing stress decreased. Two steps of critical calculations were included in the analytical model, namely, calculation of fracture-induced stresses, calculation of casing total stresses. Based on the fracture mechanics, the fracture-induced stresses under the stress-shadow were respectively calculated in reservoir plane and vertical profile. Then, the fracture-induced stresses were transformed into the casing additional loads, under which the casing additional stresses were calculated. Adding the casing additional stresses to initial stresses, the casing total stresses were obtained under densified-multi-cluster fracturing. Furthermore, the analytical model was validated by comparison of analytical results to numerical results on fracture-induced stresses and casing stresses, with the maximum deviation less than around 7.4 %. By the analytical model, it can be found that the reservoir re-distributed stresses (i.e., casing loads) were decreased and furthermore the casing stresses were decreased with increasing of fracture cluster amounts, which validates the phenomenon of casing deformation relieved under densified-multi-cluster fracturing. Finally, the mechanism of casing deformation relieved was comprehensively revealed. [ABSTRACT FROM AUTHOR]

Inducible heat shock proteins (HSPs), regulated by heat shock factor-1 (HSF-1), protect against renal cell injury in vitro. To determine whether HSPs ameliorate ischemic renal injury in vivo, HSF-1 functional knockout mice (HSF-KO) were compared with wild-type mice following bilateral ischemic renal injury. Following injury, the kidneys of wild-type mice had the expected induction of HSP70 and HSP25; a response absent in the kidneys of HSF-KO mice. Baseline serum creatinine was equivalent between strains. Serum creatinine at 24 h reflow in HSF-KO mice was significantly lower than that in the wild type. Histology showed similar tubule injury in both strains after ischemic renal injury but increased medullary vascular congestion in wild-type compared with HSF-KO mice. Flow cytometry of mononuclear cells isolated from kidneys showed no difference between strains in the number of CD4+ and CD8+ T cells in sham-operated animals. At 1 h of reflow, CD4+ and CD8+ cells were doubled in the kidneys of wild-type but not HSF-KO mice. Foxp3+ T-regulatory cells were significantly more abundant in the kidneys of sham-operated HSF-KO than wild-type mice. Suppression of CD25+Foxp3+ cells in HSF-KO kidneys with the anti-CD25 antibody PC61 reversed the protection against ischemic renal injury. Thus, HSF-KO mice are protected from ischemic renal injury by a mechanism that depends on an increase in the T-regulatory cells in the kidney associated with altered T-cell infiltration early in reflow. Hence, stress response activation may contribute to early injury by facilitating T-cell infiltration into ischemic kidney. [ABSTRACT FROM AUTHOR]

Purpose: The purpose of this study was to demonstrate whether open reduction and internal-fixation (ORIF) was superior to radial head replacement in treatment of Mason type III radial head fractures by comparing postoperative complication rate and satisfactory rate. Methods: Clinical trials comparing ORIF with radial head replacement for Mason type III radial head fractures were reviewed published up to September 1, 2012. Methodological quality of each included trials was assessed using the Jadad scale. The analyses were performed with Cochrane RevMan software version 5.1. Results: One prospective randomized controlled trial and one comparative study involving 67 patients with 67 cases were included in this systematic review and meta-analysis. Both the forest plots of complication rate and satisfactory rate indicated statistical differences between the two surgical techniques in treatment of Mason type III radial head fractures. The complication rate was 13.9 % in patients treated with radial head replacement and 58.1 % in patients treated with ORIF. The satisfactory rate was 91.7 % in patients treated with radial head replacement and 51.6 % in patients treated with ORIF. Conclusions: Given the available evidence, radial head replacement appeared to reach better outcomes in patients with Mason type III radial head fractures followed 5 years or less. Level of evidence: Therapeutic II. [ABSTRACT FROM AUTHOR]

Muscovy duck-origin goose parvovirus (MDGPV) is a new virus resulting from the natural recombination of Muscovy duck parvovirus (MDPV) and goose parvovirus (GPV). Previously identified MDGPV strains were found to have two recombination regions, one in the P9 promoter to the NS region and one in the VP3 gene, or only one recombination in the VP3 gene. In 2022, a novel strain of MDGPV known as 2022FZ was identified from China's mainland. Complete genome sequence analysis showed that there were three recombination regions in this strain: one located in the P9 promoter-NS (425–612 nt) region, one in the NS2 (1,483–1,824 nt) region, and one in the VP3 (3,124–4,248 nt) region, respectively. The recombination regions in the P9 promoter-NS, NS2, and VP3 genes were substituted with the relevant GPVs sequences, whereas the MDPV virulent strain served as the skeleton in this instance. In addition, the 2022FZ strain had multiple unique aa mutations in the NS protein and the VP protein. The Muscovy duckling challenge test showed that MDGPV-2022FZ is less pathogenic to Muscovy ducklings than two recombinant or sole recombinant MDGPV strains. For the first time, our study identified a three-region recombinant MDGPV strain and detected the novel recombination event in the NS2 gene. These results contribute to our understanding of the pathogenicity and genetic diversity of duck parvovirus. [ABSTRACT FROM AUTHOR]

GDF15 has been recently recognized as a tumor-suppressive gene. However, the underlying mechanism by which GDF15 affects breast carcinogenesis is not well understood. Here, we showed that the inhibitory effect of GDF15 on cell proliferation was dependent on the nuclear localization of the protein. Dynamic translocation of GDF15 into the nucleus altered expression of a number of genes, including KISS-1, and resulted in inhibition of cell growth and invasive behavior. Using KISS-1 promoter-driven luciferase reporter and chromatin immunoprecipitation assays, we demonstrated that, in highly malignant breast cancer cells, GDF15 directly interacts with specific protein-1 (Sp1) at the Sp1-binding sites of the KISS-1 promoter, leading to upregulated KISS-1 expression. Our study indicates that nuclear GDF15 could serve as a transcriptional coactivator to mediate the expression of particular genes to reduce cell proliferation. [ABSTRACT FROM AUTHOR]

We had previously identified that the co‐expression of transmembrane CXCL16 (TM‐CXCL16) and its receptor CXCR6 is an independent risk factor for poor survival in patients with diffuse large B‐cell lymphoma (DLBCL). However, the impact of the soluble form of CXCL16 (sCXCL16) on the pathogenesis of DLBCL remains unknown. In the present study, the synergistic effect of sCXCL16 and tumor necrosis factor α (TNF‐α) on apoptosis in DLBCL cell lines (OCI‐LY8 and OCI‐LY10) was investigated in vitro. sCXCL16 reinforced TNF‐α‐mediated inhibition of DLBCL cell proliferation, as determined by the cell counting kit‐8 assay. The results of annexin V staining showed that sCXCL16 enhanced TNF‐α‐induced apoptosis in OCI‐LY8 and OCI‐LY10 cells through a death receptor‐caspase signaling pathway. The results of gene microarray suggested a significant upregulation of differentially expressed genes in the TNF signaling pathway. sCXCL16 increased the concentration of extracellular TNF‐α by binding to CXCR6 to activate the nuclear factor‐κB (NF‐κB) signaling pathway. TNF‐α also induced the secretion of sCXCL16 by increasing the expression of ADAM10, which is known to cleave TM‐CXCL16 to yield sCXCL16. Moreover, bioinformatics analysis revealed that elevated TNF‐α and ADAM10 expression levels in tumor tissues predicted better survival in patients with DLBCL. Thus, our study suggests that sCXCL16 enhances TNF‐α‐induced apoptosis of DLBCL cells, which may involve a positive feedback loop consisting of TNF‐α, ADAM10, sCXCL16, and members of the NF‐κB pathway. sCXCL16 and TNF‐α may be used as prognostic markers in the clinic, and their combinational use is a promising approach in the context of DLBCL therapy. [ABSTRACT FROM AUTHOR]

Highlights • Muscovy duck reovirus (MDRV) causes infection and economic losses in the Muscovy duck industry. • CA strain was developed as a live attenuated vaccine candidate after serial passages of MDRV virulent strain MW9710. • CA strain is highly immunogenic to protect the ducks from MDRV infection. • A single dose of CA vaccine is sufficient to protect the birds from MDRV infection. • Safety and protective efficacy characteristics were considered for the development of a live attenuated vaccine against MDRV. Abstract The Muscovy duck reovirus (MDRV) is a highly pathogenic virus that causes substantial economic losses in the Muscovy duck industry. While MDRV poses a significant threat to Muscovy ducklings, no vaccine candidates are available to date to alleviate MDRV infection throughout the world. The present study presents efforts toward establishing an attenuated vaccine for MDRV. For this purpose, a live attenuated vaccine strain named CA was obtained via alternate propagation of the MDRV isolate MW9710 in both Muscovy duck embryo fibroblasts (MDEFs) and chicken embryo fibroblasts (CEFs) for 90 passages. The CA strain achieved an adaptive growth capacity in CEFs with a viral titer that ranged between 105.0–105.5 TCID 50 /100 μL and lost its pathogenicity in 1-day-old Muscovy ducklings. Compared to the parent strain MW9710, the CA strain has 42 scattered amino acid substitutions, most of which are located in the λB, λC, μB, σB, and σC protein. The CA strain maintained its attenuation and showed no gene mutation or virulence reversion after back propagation into 1-day-old ducklings for five rounds. The minimum protective dose was calculated to be 300 TCID 50 of the CA strain. Furthermore, a single dose of CA vaccine protected immunized ducklings against lethal challenge by the virulent MDRV strain MW9710 and significantly decreased viral loads. In summary, the CA strain exhibited striking genetic stability, excellent safety, and effective immunogenicity. This CA strain of MDRV is a promising vaccine candidate for the prevention and control of MDRV infection. [ABSTRACT FROM AUTHOR]

Purpose: A subset of common variable immunodeficiency (CVID) patients either presents with or develops autoimmune and lymphoproliferative complications, such as granulomatous lymphocytic interstitial lung disease (GLILD), a major cause of morbidity and mortality in CVID. While a myriad of phenotypic lymphocyte derangements has been associated with and described in GLILD, defects in T and B cell antigen receptor (TCR/BCR) signaling in CVID and CVID with GLILD (CVID/GLILD) remain undefined, hindering discovery of biomarkers for disease monitoring, prognostic prediction, and personalized medicine approaches. Methods: To identify perturbations of immune cell subsets and TCR/BCR signal transduction, we applied mass cytometry analysis to peripheral blood mononuclear cells (PBMCs) from healthy control participants (HC), CVID, and CVID/GLILD patients. Results: Patients with CVID, regardless of GLILD status, had increased frequency of HLADR+CD4+ T cells, CD57+CD8+ T cells, and CD21lo B cells when compared to healthy controls. Within these cellular populations in CVID/GLILD patients only, engagement of T or B cell antigen receptors resulted in discordant downstream signaling responses compared to CVID. In CVID/GLILD patients, CD21lo B cells showed perturbed BCR-mediated phospholipase C gamma and extracellular signal-regulated kinase activation, while HLADR+CD4+ T cells and CD57+CD8+ T cells displayed disrupted TCR-mediated activation of kinases most proximal to the receptor. Conclusion: Both CVID and CVID/GLILD patients demonstrate an activated T and B cell phenotype compared to HC. However, only CVID/GLILD patients exhibit altered TCR/BCR signaling in the activated lymphocyte subsets. These findings contribute to our understanding of the mechanisms of immune dysregulation in CVID with GLILD. [ABSTRACT FROM AUTHOR]

Duck spleen marble-like necrosis disease (DSMND) has been prevalent in Chinese duck farms since 2016. The etiological study was carried out in this study using etiological detection, pathogen isolation, whole genome sequencing, and artificial infection test. A highly pathogenic Riemerella anatipestifer (RA) strain was determined as the etiologic agent. Phylogenomic analysis showed that this RA strain was closely related to duck origin RA strain RCAD0421 and chicken origin RA strain S63. The clinical symptoms and pathological changes of artificial infection ducks were similar to those of clinical cases. This is the first identification of RA as the pathogen of DSMND, which provides a theoretical basis for this disease' s prevention and control. • An unidentified disease designated duck spleen marble-like necrosis disease (DSMND). • The viral pathogens were excluded as the causative agent for DSMND. • The causative agent of DSMND was identified as a kind of high pathogenicity RA strain. [ABSTRACT FROM AUTHOR]

A new disease designated as Pale liver disease (PLD) has been circulating in Chinese Muscovy duck flocks since 2014, which is characterized by fatigue, diarrhoea, sudden death and acute hepatitis with pale and haemorrhagic liver. In this study, the etiological agents of PLD were isolated, causing a significant cytopathic effect (CPE) by cell rounding. Virus particles were observed by transmission electron microscopic (TEM) observation. The same disease was reproduced by experimental infection with the isolate BG61. The whole genomes of isolates were 43,842 nt in length with a GC content of 47.11%, similar to French Muscovy duck adenovirus strain GR with a GC content of 46.08%. The isolates shared 99.71–99.95% and 93.31–93.33% identity with Chinese Muscovy duck adenovirus isolates and GR strain, respectively. The DNA polymerase gene of all Muscovy duck adenovirus strains formed a separate genetic lineage with 99.55–100% amino acid sequence identity. All Chinese Muscovy duck adenovirus isolates contained two fibre genes. In contrast, only one fibre gene was found in GR, the only representative strain in species Duck aviadenovirus B. Anti‐DAdV‐2 serum antibodies had a weak neutralizing activity against Chinese Muscovy duck adenovirus isolates. The phylogenetic trees of the complete genome, hexon and fibre proteins revealed that all Muscovy duck adenovirus strains formed a major genetic lineage consisting of two clades. Thus, both GR and Chinese Muscovy duck adenovirus strains were proposed to be included in the same species of Duck aviadenovirus B belonging to the genus Aviadenovirus. The species Duck aviadenovirus B included two serotypes or genotypes, such as GR, which represents the strain of serotype 1 or genotype 1 (DAdV B1) and Chinese Muscovy duck adenovirus strains, which belong to serotype 2 or genotype 2 (DAdV B2). [ABSTRACT FROM AUTHOR]

To investigate the possible role of recombination in the evolution of Muscovy duck parvovirus (MDPV) and goose parvovirus (GPV) in Taiwan, we analyzed a potentially significant recombination event that occurred only in GPV by comparing thirteen complete sequences of the capsid gene VP2 of GPV and MDPV. The recombination event occurred between GPV strain 06-0239 as the minor parent and strains 99-0808 as the major parent, which resulted in the GPV recombinant V325/TW03. GPV V325/TW03 is likely to represent a new genotype among the Taiwanese GPV strains. This represents the first evidence that intergenotype recombination within the VP2 gene cluster contributes to the genetic diversity of the VP2 genes of Taiwanese GPV field strains. [ABSTRACT FROM AUTHOR]

Duckling short beak and dwarfism syndrome virus (SBDSV), an emerging goose parvovirus, has caused short beak and dwarfism syndrome (SBDS) in Chinese duck flocks since 2015. Presently, there is no commercial vaccine against SBDS. In the present study, a virus‐like particle (VLP)‐based candidate vaccine was developed against this disease. A baculovirus expression system was used to express the SBDSV VP2 protein in Sf9 cells. Immunofluorescence assay, sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS‐PAGE) and Western blotting were used to confirm protein expression. Furthermore, transmission electron microscopy was used to observe the formation of VLPs. VLPs were formulated into an oil‐adjuvanted maternal vaccine to evaluate humoral responses in breeding ducks via latex particle agglutination inhibition assay (LPAI) and microneutralization assay. The offspring were challenged with SBDSV to test the protective efficacy. A single dose of SBDSV was able to induce the high level of LPAI antibodies in ducks, with LPAI and neutralization peak titres of 4.9 ± 1.20 log2 and 7.1 ± 1.20 log2, respectively, at 4 weeks post‐vaccination (wpv). The average LPAI titre of yolk antibodies in duck eggs receiving 2 doses (first and boost doses) of the vaccine was 5.3 ± 1.09 log2 at 4 weeks post‐boost. The protective efficacy of the maternal vaccine was 87.5%–100%. These results indicate that SBDSV VLPs can be a promising vaccine candidate for controlling SBDS. [ABSTRACT FROM AUTHOR]

Muscovy duck reovirus (MDRV) is highly pathogenic to young Muscovy ducklings. Although MDRV infection results in ducklings' acute watery diarrhea, the effect of MDRV infection on the composition of host's intestinal microbiota remains poorly understood. This study was conducted to investigate the impacts of MDRV on the composition of Muscovy ducklings' intestinal bacterial community. Three-day-old Muscovy ducklings were inoculated with either the virulent MDRV strain MW9710 or sterile Hank's solution, respectively. The cecal microbiota was analyzed between control and mock MDRV-infected ducklings using Illumina MiSeq sequencing at 6 dpi and 17 dpi, respectively. The results indicated that MDRV infection damaged the intestinal mucosa. In addition, MDRV infection caused severe perturbations of gut microbiota by decreasing microbial richness, altering the abundance of certain genera of the gut microbiota at 6 dpi. Specifically, the relative abundance of short chain fatty acids-producing bacteria (including Shuttleworthia, Streptococcus, and Ruminococcus) was reduced in MDRV-infected ducklings than those of control group, whereas, with an enrichment of Enterobacteriaceae (including Plesiomonas, Escherichia_Shigella and Proteus). Furthermore, microbiota analysis showed that the gut microbiota dysbiosis caused by MDRV infection was basically recovered at 17 dpi. Collectively, this study demonstrated that the gut microbiota of Muscovy ducklings were altered due to MDRV infection, mainly featuring as a net loss of beneficial bacteria and a compensatory proliferation of pathogenic bacteria, which may lead to severe pathology to the intestinal mucosa, and ultimately acute diarrhea. These results will provide insights into the pathology of MDRV infection. [ABSTRACT FROM AUTHOR]

A multiplex polymerase chain reaction (PCR) method was developed to detect and distinguish goose parvovirus (GPV), waterfowl reovirus (WRV), and goose astrovirus (GAstV). Three pairs of primers were designed based on conserved regions in the genomic sequences of these enteric viruses and were used to specifically amplify targeted fragments of 493 bp from the viral protein 3 (VP3) gene of GPV, 300 bp from the sigma A-encoding gene of WRV, and 156 bp from the capsid protein-encoding gene of GAstV. The results showed that the primers can specifically amplify target fragments, without any cross-amplification with other viruses, indicating that the method had good specificity. A sensitivity test showed that the detection limit of the multiplex PCR method was 1 × 103 viral copies. A total of 102 field samples from Muscovy ducks with clinically suspected diseases were evaluated using the newly developed multiplex PCR method. The ratio of positive samples to total samples for GPV, WRV, and GAstV was 73.53% (75/102) for multiplex PCR and was 73.53% (75/102) for routine PCR. Seventy-five positive samples were detected by both methods, for a coincidence ratio of 100%. This multiplex PCR method can simultaneously detect GPV, WRV, and GAstV, which are associated with viral enteritis, thereby providing a specific, sensitive, efficient, and accurate new tool for clinical diagnosis and laboratory epidemiological investigations. • A multiplex PCR assay was developed for simultaneous detection for GPV, WRV, and GAstV in Muscovy duck flocks, with good specificity, good accuracy, and high sensitivity. • The multiplex PCR was able to detect at least 1 × 103 viral copies of GPV, WRV, and GAstV. • The multiplex PCR developed is a useful tool for clinical diagnosis of single virus and/or mixed infections in Muscovy duck. [ABSTRACT FROM AUTHOR]

The complex Ginzburg–Landau equation (CGLE) is used to investigate the effect of a nonlocal feedback control on spiral dynamics in oscillatory media, where the feedback is derived from a square-shaped domain, and three kinds of controlled tip trajectories can be found. The tip trajectory usually tends to a square limit cycle concentric with the measuring domain when the initial tip location is near the periphery of the domain. If the spiral tip is initially located in the measuring domain, it will finally be fixed at a point in the domain. For some larger delay time, the spiral tip may have a motion pathway of small limit cycles. For the case of a square-shaped pathway, the modulus function of the feedback signal has a maximum value when the spiral tip approaches the midpoint of each side, and a slower change when the tip is close to each turning point. As the feedback gain is increased, the spiral tip moves along the same pathway with a greater speed, and multiple tips can be generated when the gain is very large. The final trajectory is also affected by the distance between the measuring center and the initial spiral tip, the size of the measuring domain, the delay time, the choice of positive and negative gains and the scaling parameter. [ABSTRACT FROM AUTHOR]

Abstract To obtain a deletion mutant of Muscovy duck-origin goose parvovirus (MDGPV) and to analyze its biological characteristics, the pMDGPVPT plasmid, which contains a full-length DNA infectious clone of the MDGPV PT strain, was used in this study as the template. The E-box at nt 315 of the left inverted terminal repeat sequence (L-ITR) was deleted by overlap extension PCR to obtain the infectious recombinant plasmid p-PTΔE315. The p-PTΔE315 plasmid was transfected into 9-day-old non-immune Muscovy duck embryos via the yolk sac and the rescued deletion mutant virus r-PTΔE315 was generated. Experiments to demonstrate the novel deletion mutant virus' biological characteristics showed that r-PTΔE315 can cause typical lesions after infection of Muscovy duck embryos. Compared with its parent strain PT, the virulence of r-PTΔE315 and its proliferation ability in Muscovy duck embryos were attenuated, but its ability to replicate in MDEF cells was enhanced. This study laid the foundation for further understanding of the relationship between E-box deletion in the L-ITR and MDGPV virulence. Highlights • In this study, we constructed a full-length infectious clone (pMDGPVPT). • Deletion of E-box (E315) may improve the adaptability of MDGPV to MDEF cells. • Deletion of E-box (E315) decreased MDGPV replication in Muscovy duck embryos. [ABSTRACT FROM AUTHOR]

Spiral dynamics in the oscillatory bilayer system with an inhomogeneous inter-layer coupling and a spiral-resting initial state are studied, where the square coupled patches are arranged into a square lattice array. Depending on the patch side length, the lattice space and the coupling strength, different dynamical behaviors can be observed. The complete synchronization of two spiral waves easily occurs when the coupled areas occupy a larger proportion in the whole region. The bilayer system can also show several typical asynchronous patterns, where the spiral with the closed tip path, the spiral with the complex phase shifting, the state with multiple spirals, the spiral turbulence, and the non-smooth traveling pulses can be formed in the single layers of the bilayer. The variable difference functions are computed, and three types of asymptotic behaviors are identified, which include the behaviors of approaching the constant, periodic variation, and aperiodic variation around the constant. These asymptotic behaviors can describe some properties of the bilayer patterns after a transient interval. For example, the variable difference function has a periodical asymptotic behavior when the spiral with the closed tip path is formed in the single layer. The heterogeneity of inter-layer coupling can also induce some complex tip trajectories. • Inhomogeneous inter-layer coupling can cause phase synchronization by two ways. • Typical patterns including non-smooth traveling waves are identified and analyzed. • Variable difference functions have three types of asymptotic behaviors. • Periodic asymptotic behaviors correspond to the spirals with closed tip paths. • Constant asymptotic behaviors correspond to the spirals with complex phase shifting. [ABSTRACT FROM AUTHOR]

Till now, salt precipitation occurring in fault zone, named fault-induced salt precipitation, has not been reported for CO 2 storage, although mineral precipitation has been reported occurring in Little Grand Wash Fault zone due to co-leakage of CO 2 and water. Salt precipitation in fault zone can play critical roles to prevent CO 2 leakage along fault, because the activated fault can be sealed by salt precipitation. In view of this, it would be investigated whether the salt precipitation can occur in fault and concluded for the evolution of fault-induced salt precipitation, especially considering convection of CO 2 and brine along fault during CO 2 storage in multilayered saline aquifer-caprock. In detail, the theoretical model was established to solve the solid saturation based on mass and energy conservation equations. By simplifying the discretized mass conservation equation, it could be found that salt precipitation was controlled by brine migration rate. Then, the physical model was proposed considering the convection of CO 2 and brine along the fault, formed by CO 2 migrating upwards and brine migrating downwards along fault. Furthermore, the numerical model was established to validate salt precipitation occurring in fault zone. The numerical results showed that: (i) the convection of CO 2 migrating upwards and brine migrating downwards could be observed from velocity vectors of fluid migration; (ii) slow migration of brine could be found by comparing migration rates of CO 2 and brine; (iii) salt precipitation indeed occurred in fault zone. By the numerical results, the evolution of salt precipitation was divided into three stages, namely, Stage I: convection of CO 2 and brine; Stage II: slow migration of brine; Stage III: salt precipitation occurring. Finally, the parametric analyses were performed to investigate the effects of different parameters on salt precipitation. Results indicated that increasing in the injection rate and the fault height but decreasing in the injection time interval, the fault dip angle and the distance between fault and injection well were more beneficial for the salt precipitation in fault. The findings of this study can help for better understanding of the phenomenon of salt precipitation occurring in fault. • Phenomenon of fault-induced salt precipitation due to convection of CO 2 and brine was reported. • Evolution process was concluded into three stages, including precondition and control condition. • Effects of different parameters on salt precipitation and CO 2 migration rate were investigated. [ABSTRACT FROM AUTHOR]

Short beak and dwarfism syndrome virus (SBDSV) is a newly emerging distinct duck-origin goose parvovirus that belongs to the genus Dependovirus. Our previous studies have found that SBDSV was highly pathogenic to Cherry Valley ducklings and mule ducklings. However, little is known about its pathogenicity to other waterfowls. In the present study, the pathogenicity of SBDSV was evaluated in domesticated waterfowl including Muscovy ducklings, Sheldrake ducklings and domestic goslings. All experimentally infected birds exhibited remarkable growth retardation, anorexia and diarrhea similar to naturally infected birds. Interestingly, atrophic beaks and protruded tongues were not observed in all infection groups. At necropsies, no diagnostic pathological lesions were observed. Viral antigens existed in most organ tissues such as heart, liver, spleen, kidney, pancreas and intestine. All ducks in Muscovy duckling and Sheldrake duckling infected groups and 70% goslings in infected groups were seropositive for goose parvovirus (GPV) antibodies at 21 dpi with the average titers as 2 8.4 , 2 6.9 , 2 4.0 , respectively. Muscovy ducklings were more prominent in viral load and weight loss with a higher GPV antibodies titer than Sheldrake ducklings and goslings. Taken together, SBDSV exhibits a wide range of pathogenicity to main domesticated waterfowl with variable symptoms and cause considerable economic losses in China. [ABSTRACT FROM AUTHOR]

Many mule duck and Cherry Valley duck flocks in different duck-producing regions of China have shown signs of an apparently new disease designated 'short beak and dwarfism syndrome' (SBDS) since 2015. The disease is characterized by dyspraxia, weight loss, a protruding tongue, and high morbidity and low mortality rates. In order to characterize the etiological agent, a virus designated SBDSV M15 was isolated from allantoic fluid of dead embryos following serial passage in duck embryos. This virus causes a cytopathic effect in duck embryo fibroblast (DEF) cells. Using monoclonal antibody diagnostic assays, the SBDSV M15 isolate was positive for the antigen of goose parvovirus but not Muscovy duck parvovirus. A 348-bp (2604-2951) VP1gene fragment was amplified, and its sequence indicated that the virus was most closely related to a Hungarian GPV strain that was also isolated from mule ducks with SBDS disease. A similar disease was reproduced by inoculating birds with SBDSV M15. Together, these data indicate that SBDSV M15 is a GPV-related parvovirus causing SBDS disease and that it is divergent from classical GPV isolates. [ABSTRACT FROM AUTHOR]

Spiral waves and pulses in the excitable medium with an anomalous diffusion are studied. In the medium with an one-sided fractional diffusion in the -direction and a normal diffusion in the -direction, a pulse, traveling along the positive -direction, has a smaller velocity, which is different from the diffusion of a source in the other media. Its propagating velocity is a linear and increasing function of the square root of diffusion parameter, whose increasing rate depends on the fractional order. A minimal value of the diffusion parameter is needed for successfully propagating pulses, and the threshold becomes large with a decrease of the fractional order. For pulse trains, the frequency-locked bands are shifted along the increasing direction of the perturbation period when the fractional order is decreased. In the propagating process of a spiral wave, the tip drift is induced by the one-sided fractional diffusion, which may be explained by analyzing the SV area in front of the tip. [ABSTRACT FROM AUTHOR]

ZBP1-modulated localization of β-actin mRNA enables a cell to establish polarity and structural asymmetry. Although the mechanism of β-actin mRNA localization has been well established, the underlying mechanism of how a specific molecular motor contributes to the transport of the ZBP1 (also known as IGF2BP1) complex in non-neuronal cells remains elusive. In this study, we report the isolation and identification of KIF11, a microtubule motor, which physically interacts with ZBP1 and is a component of β-actin messenger ribonucleoprotein particles (mRNPs). We show that KIF11 colocalizes with the β-actin mRNA, and the ability of KIF11 to transport β-actin mRNA is dependent on ZBP1. We characterize the corresponding regions of ZBP1 and KIF11 that mediate the interaction of the two proteins in vitro and in vivo. Disruption of the in vivo interaction of KIF11 with ZBP1 delocalizes β-actin mRNA and affects cell migration. Our study reveals a molecular mechanism by which a particular microtubule motor mediates the transport of an mRNP through direct interaction with an mRNA-binding protein. [ABSTRACT FROM AUTHOR]

Tembusu virus (TMUV) has been a causative agent of an acute egg-drop syndrome found in Chinese duck populations since at least 2010. In this paper, we report the characterization of a TMUV-like flavivirus (named CJD05) isolated from naturally infected egg-laying fowl. The virus was identified and then isolated from hens suffering from severe egg drop and fever in Fujian Province, China. The virus replicated well in MDEF and CEF cells, and its cytopathogenic effect (CPE) was apparent. Hemagglutinating activity (HA) was negative for this virus using erythrocytes from both chickens and pigeons. Viral particles were enveloped and approximately 45 nm in diameter, as observed by electron microscopy. Phylogenetic analysis of the full-length nucleotide sequence of CJD05 indicated that this virus is closely related to the duck-origin TMUV, belonging to Ntaya group of flavivirus. Most importantly, pathogenicity studies showed that CJD05 is highly virulent in 1-day-old chicks, 1-day-old Muscovy ducks, egg-laying chickens and shelducks. Our research highlights the increase in epidemic disease caused by avian TMUV, and subsequent outbreaks are becoming more complicated to treat. The pathogenic mechanisms of the virus are still not fully understood, further research is needed. [ABSTRACT FROM AUTHOR]

Brown Norway rats (BN, BN/NHsdMcwi) are profoundly resistant to developing acute kidney injury (AKI) following ischemia reperfusion. To help define the genetic basis for this resistance, we used consomic rats, in which individual chromosomes from BN rats were placed into the genetic background of Dahl SS rats (SS, SS/JrHsdMcwi) to determine which chromosomes contain alleles contributing to protection from AKI. The parental strains had dramatically different sensitivity to ischemia reperfusion with plasma creatinine levels following 45 min of ischemia and 24 h reperfusion of 4.1 and 1.3 mg/dl in SS and BN, respectively. No consomic strain showed protection similar to the parental BN strain. Nine consomic strains (SS-7BN, SS-XBN, SS-8BN, SS-4BN, SS-15BN, SS-3BN, SS-10BN, SS-6BN, and SS-5BN) showed partial protection (plasma creatinine about 2.5-3.0 mg/dl), suggesting that multiple alleles contribute to the severity of AKI. In silico analysis was performed using disease ontology database terms and renal function quantitative trait loci from the Rat Genome Database on the BN chromosomes giving partial protection from AKI. This tactic identified at least 36 candidate genes, with several previously linked to the pathophysiology of AKI. Thus, natural variants of these alleles or yet-to-be identified alleles on these chromosomes provide protection against AKI. These alleles may be potential modulators of AKI in susceptible patient populations. [ABSTRACT FROM AUTHOR]

• A TaqMan-MGB probe-based one-step real-time RT-PCR assay for the rapid detection of H146-like GCV was developed. • The assay in this study was simple and highly specific. • This approach will help to provide knowledge on epidemiological study focused on H146-like GCV infection. H146-like goose-origin calicivirus (H146-like GCV) is a novel Caliciviridae family member in the Sanovirus genus that was associated with gosling growth retardation syndrome growth retardation syndrome complicated by visceral urate deposition. However, there is no accurate and high throughput real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) available for the rapid and highly sensitive identification of H146-like GCV. In this study, a pair of specific primers and a TaqMan minor groove binder (MGB) probe were designed based on a conserved region in the nonstructural (NS) gene sequence. The TaqMan-MGB probe-based one-step qRT-PCR assay was capable of detecting quite low number of targeting nucleic acid as low as 5.07 copies/μL and had excellent intra-assay and inter-assay repeatability with the coefficient of variation (CV) value from 0.558% to 1.293%. The assay was highly specific for H146-like GCV, without cross-reactions with other non-targeted goose-origin viruses, and 62 suspicious tissue samples infected with H146-like GCV from different regions of Fujian Province were used in this study to verify the feasibility and effectiveness of this assay in clinical diagnosis. The results indicated that our assay for the diagnosis and quantification of H146-like GCV was highly sensitive and specific, and should provide a reliable real-time tool for epidemiological and pathogenetic study of H146-like GCV infection, enabling researchers to better understand the epidemiology and clinical presentation of this disease. [ABSTRACT FROM AUTHOR]

The complete sequence of a reovirus, strain NP03 associated with necrotic focus formation in the liver and spleen of Muscovy ducklings in Fujian Province, China in 2009, was determined and compared with sequences of other waterfowl and chicken-origin avian reoviruses (ARVs). Sequencing of the complete genomes of strain NP03 showed that they consisted of 23,418 bp and were divided into 10 segments, ranging from 1191 bp (S4) to 3959 bp (L1) in length, and all segments contained conserved sequences in the 5′ non-coding region (GCUUUU) and 3' non-coding region (UCAUC). Pairwise sequence comparisons demonstrated that NP03 strain showed the highest similarity with novel waterfowl origin reoviruses (WRVs). The genome analysis revealed that the S1 segment of novel WRV is a tricistronic gene, encoding the overlapping open reading frames (ORFs) for p10, p18, and σC, similar to the ARV S1 gene, but distinct from classical WRV S4 genome segment, which contained two overlapping ORFs encoding p10 and σC. Phylogenetic analyses of the nucleotide sequences of all 10 segments revealed that NP03 strain was clustered together with other novel WRVs and were distinct from classical WRVs and chicken-origin ARVs. The analyses also showed possible intra-segmental reassortment events in the segments encoding λA, λB, μB, μNS, σA, and σNS between novel and classical WRVs. Potential recombination events detection in segment L1 suggests that NP03 strain may be recombinants of novel WRVs. Based on our genetic analyses, multiple reassortment events, intra-segmental recombination, and accumulation of point mutations have possibly contributed to the emergence of this novel genotype of WRV, identified in China. • There were two genotypes of waterfowl reovirus (WRV), which mainly causes the outbreaks in China. • The novel WRV strain NP03 likely derived from multiple reassortment events. • Possible intra-segmental recombination event was identified. [ABSTRACT FROM AUTHOR]

A real-time reverse transcriptase-polymerase chain reaction (RT-PCR) for the detection of Muscovy duck reovirus (MDRV) RNA in clinical samples is described. The assay is based on Taq Man-MGB technology, consisting of two primers and one probe labeled with the reporter dye 6-carboxyfluorescein that binds selectively to the sigma B-protein gene of MDRV. This technique also includes an Internal Positive Control (IPC). The real-time RT-PCR assay was able to detect MDRVs, whereas other common waterfowl-origin viral pathogens were not recognised by the established oligonucleotide set, thus showing that the test was specific for MDRV. The sensitivity of the assay was 2.83 × 101 copies/μL and was 100 times higher than that of the conventional RT-PCR. The variation coefficients of intra-assay and inter-assay were less than 1.5% which verified sufficient repeatability of this assay. The use of β-actin mRNA as an IPC in order not to reduce the efficiency of the assay was adopted. The detection for 100 clinical samples showed that the positive rate of the established Taq Man-MGB real-time RT-PCR method was 87% (87/100), while the positive rate of the conventional RT-PCR was 83% (83/100), with the coincidence rate was 97.14%. Sensitivity and positive rate for clinical samples of Taq Man fluorescent quantitative RT-PCR were higher than conventional RT-PCR. The high specificity, sensitivity, and rapidity Taq Man-MGB real-time RT-PCR assay with the use of IPC to monitor for false negative results can make this method suitable for the pathogenic surveillance and epidemiological investigation of MDRV infection. • A Taq Man-MGB real-time RT-PCR was developed for detection MDRV. • The assay includes an internal control of the β-actin gene. • The Taq Man-MGB real-time RT-PCR offered rapid, effective and reliable for the detection of MDRV in Muscovy ducks. [ABSTRACT FROM AUTHOR]

An unclassified calicivirus (CV) detected in geese was recently reported and proposed as a new member of the family Caliciviridae. There is limited information about the epidemiology, etiology and detection method of goose-origin CV (GCV) to date. In this study, an EvaGreen based fluorescence quantitative real-time RT-PCR assay was developed and optimized for the detection of GCVs. The assay sensitively detected GCV RNA template with a good linear standard curve. We also demonstrated the specificity and reproducibility of the detection method for GCVs. Thus, the method developed in this study will benefit the investigation of possible sporadic outbreaks of CV infections in geese, as well as epidemiological and etiological studies of GCVs. • An EvaGreen based real-time RT-PCR assay was developed for detection and quantitation of newly emerging goose caliciviruses. • The assay provides quantitative data that may be used to inform epidemiological and etiological studies. [ABSTRACT FROM AUTHOR]

Duckling short beak and dwarfism syndrome virus (SBDSV), a newly identified goose parvovirus, causes devastating disease in domestic waterfowl and considerable economic losses to Chinese waterfowl industry. The molecular pathogenesis of SBDSV infection, nature and dynamics of host immune responses against SBDSV infection remained elusive. In this study, we systematically explored the relative mRNA expression profiles of major innate immune-related genes in SBDSV infected duck embryo fibroblasts. We found that SBDSV infection effectively activated host innate immune responses and resulted in significant up-regulation of IFN-β and several vital IFN-stimulated genes (ISGs). These up-regulation responses were mainly attributed to viral genomic DNA and dsRNA replication intermediates. Importantly, the expression of cGAS was significantly induced, whereas the expression of other DNA receptors including DDX41, STING, ZBP1, LSM14A and LRRFIP1 have no significant change. Furthermore, SBDSV infection also activates the up-regulation of TLR3 and inhibited the expression of TLR2 and TLR4; however, no effect was observed on the expression of TLR1, TLR5, TLR7, TLR15 and TLR21. Intriguingly, SBDSV infection significantly up-regulated the expression of RNA sensors such as MDA5 and LGP2, and resulted in a delayed but significant up-regulation of RIG-I gene. Taken together, these data indicate that host multiple sensors including DNA sensor (cGAS) and RNA sensors (TLR3, MDA5 and LGP2) are involved in recognizing a variety of different pathogen associated molecular patterns (PAMPs) including viral genomic ssDNA and dsRNA replication intermediates, which trigger an effective antiviral innate immune response. • SBDSV, a new number of goose parvovirus, causes devastating disease in domestic waterfowl. • The relative mRNA expression profiles of innate immune-related genes in SBDSV infected DEFs were systematically explored. • SBDSV infection activates host innate immune responses and results in significant up-regulation of IFN-β and vital ISGs. • Host multiple sensors are involved in recognizing pathogen associated molecular patterns (PAMPs). [ABSTRACT FROM AUTHOR]

Novel duck reovirus (NDRV) is pathogenic to young ducks, which is characterized by hemorrhagic spots and necrotic foci of the livers and necrotic foci of spleens. However, the effect of NDRV infection on the composition of the host's intestinal microbiota remains poorly understood. In this study, three-day-old Muscovy ducklings were inoculated with either the virulent NDRV strain NP03 or sterile Hank's solution. Through Illumina MiSeq sequencing, the whole cecal microbiota of healthy and NDRV infected ducklings was examined. The results showed that the gut microbiota was mainly dominated by Firmicutes, Proteobacteria and Bacteroidestes in both healthy and NDRV infected ducks. NDRV infection altered the relative abundance of bacteria. Specifically, families Ruminococcaceae and Lachnospiraceae were remarkably reduced, whereas Escherichia_Shigella belonging to family Enterobacteriaceae was significantly increased. Collectively, NDRV infection in Muscovy ducks resulted in a shift of the gut microbiota, including a net loss of probiotic bacteria with a compensatory expansion of pathogenic bacteria. These results provide new insights into the potential pathogenic mechanisms of NDRV. • The gut microbiota of Muscovy ducklings was mainly dominated by Firmicutes, Proteobacteria and Bacteroidestes. • NDRV infection remarkably reduced the abundance of families Ruminococcaceae and Lachnospiraceae. • The relative abundance of Escherichia_Shigella was significantly increased after NDRV infection. [ABSTRACT FROM AUTHOR]

Muscovy duck-origin goose parvovirus (MDGPV) is a causative agent of MDGPV-associated Derzsy's disease. To evalute the role of the cis -acting element E-box (CACATG) deletion on MDGPV eplication, an infectious plasmid clone p-PTΔE287, having one E-box deletion at nucleotide (nt) 287 of the left inverted terminal repeat sequence (L-ITR), was constructed by overlap extension PCR deleting the 287CACATG292 motif from the plasmid pMDGPVPT containing the full-length genome of the virulent MDGPV strain PT. The p-PTΔE287 plasmid was transfected into 9-day-old non-immune Muscovy duck embryos via the yolk sac, resulting in successful rescue of the deletion mutant virus r-PTΔE287. Compared with its parental virus PT, the virulence and the replication ability of r-PTΔE287 were reduced. In addition, we examined the ability of r-PTΔE287 to manipulate cell cycle progression. The results showed that r-PTΔE287 replication results in G0/G1 phase accumulation of infected duck embryo liver mesenchymal stem cells (BMSCs) and that this accumulation is caused by the prevention of cell cycle entry from G0/G1 phase into S phase. Taken together, introducing 287CACATG292 element deletion into MDGPV PT genomic DNA that induced rescued mutant virus (r-PTΔE287) cell cycle arrest function at the G0/G1 phase, which might inhibit MDGPV replication and virus progeny production. This study laid the foundation for further understanding of the relationship between E-box deletion in the L-ITR and MDGPV virulence. • We constructed an E-box motif (CACATG)-deleted MDGPV. • Deletion of E-box (E287) decreased MDGPV replication in Muscovy duck embryos. • The mutant virus r-PTΔE287 may induce cell cycle arrest at G0/G1 phase. [ABSTRACT FROM AUTHOR]

Background: Waterfowl parvoviruses, including goose parvovirus (GPV) and Muscovy duck parvovirus (MDPV), can cause seriously diseases in geese and ducks. Developing a fast and precise diagnosis assay for these two parvoviruses is particularly important. Results: A duplex SYBR Green I-based quantitative real-time PCR assay was developed for the simultaneous detection and differentiation of GPV and MDPV. The assay yielded melting curves with specific single peak (Tm = 87.3 ± 0.26 °C or Tm = 85.4 ± 0.23 °C) when GPV or MDPV was evaluated, respectively. When both parvoviruses were assessed in one reaction, melting curves with specific double peaks were yielded. Conclusion: This duplex quantitative RT-PCR can be used to rapid identify of GPV and MDPV in field cases and artificial trials, which make it a powerful tool for diagnosing, preventing and controlling waterfowl parvovirus infections. [ABSTRACT FROM AUTHOR]