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The objective was to examine risk factors for the interval to resumption of ovarian cyclicity (ROC), multiple ovulations (MCL), ovarian follicular cysts (OC), and presence of intrauterine fluid (IUF) at 22 to 28 [visit (V) 1] and 36 to 42 (V2) days in milk (DIM) in dairy cows. The study was conducted retrospectively by evaluating records from 1,155 Holstein cows from 3 herds. Ovaries and uteri were examined at V1 and V2 by transrectal ultrasonography to determine ovarian structures and IUF. Based on the odds ratio, multiparous cows were more likely to have ROC at V1 by a factor of 1.79 compared with primiparous cows. The likelihood of ROC at V1 was lower in cows with higher milk production, in cows with retained fetal membranes (RFM) or cows with IUF at V1 by factors of 0.98 (for each kg of milk increased), 0.52, and 0.61, respectively. Based on the odds ratio, cows diagnosed with IUF at V2 were 2.85 times more likely to have attained ROC at V2. Multiparous cows and cows that delivered twins were 2.73 and 2.16 times, respectively, more likely to have MCL at V1, whereas cows with RFM were 0.38 times less likely to have MCL at V1. The likelihood of MCL at V2 was higher in cows with MCL and OC at V1 by factors of 2.67 and 1.91, respectively. Multiparous cows were 8.51 times more likely to have OC at V1 than primiparous cows. Higher producing cows were more likely to have OC at V2 by a factor of 1.04 compared with lower producing cows. Parity, stillbirth, RFM, and ROC at V1 were all identified as risk factors for IUF at V1. Cows with RFM and delivering twins were more likely to be diagnosed with IUF at V2 by a factor of 3.43 and 4.07, respectively. In summary, parity, twinning, RFM, metritis, IUF, and milk production were all associated with altered ovarian structures, and the presence of IUF was related to parity, twinning, RFM, and ROC in postpartum dairy cows. [ABSTRACT FROM AUTHOR]

The objectives were to determine the effects of supplemental fat (no oilseed vs. oilseed) during late gestation and the source of fat (canola vs. sunflower seed), on dry matter intake (DMI), plasma metabolite concentrations, milk production and composition, calf birth weight, postpartum health disorders, ovarian function and reproductive performance in dairy cows. Pregnant Holstein cows, blocked by body condition and parity, were assigned to 1 of 3 diets containing rolled canola seed (high in oleic acid; n = 43) or sunflower (high in linoleic acid; n = 45) at 8% of dry matter, or no oilseed (control; n = 43), for the last 35 ± 2 d of pregnancy. After calving, all cows received a common lactation diet. Blood samples were collected at wk -3 (i.e., 2 wk after initiation of prepartum diets) and at wk +1, +2, +3, +4 and +5 postpartum to determine the concentration of fatty acids (mEq/dL), β-hydroxybutyrate (mg/dL), and glucose (mg/dL). Ovarian ultrasonography was performed twice weekly to determine the first appearance of dominant (10 mm) and preovulatory-size (≥16 mm) follicles, and ovulation. Uterine inflammatory status based on the proportion of polymorphonuclear leukocytes (PMN; subclinical endometritis: >8% PMN) was assessed at d 25 ± 1 postpartum. Significant parity by treatment interactions were observed for DMI and milk yield. Prepartum oilseed supplementation, more specifically sunflower seed supplementation, increased postpartum DMI in primiparous cows without affecting prepartum DMI or milk yield. Contrarily, in multiparous cows, prepartum oilseed supplementation decreased both prepartum and postpartum DMI and milk yield during the first 2 wk. Regardless of parity, prepartum feeding of canola reduced postpartum DMI compared with those fed sunflower. Mean fatty acids concentrations at wk -3 were greater in cows given supplemental oilseed than those fed no oilseeds. Gestation length and calf birth weight were increased in cows given supplemental oilseed prepartum compared with cows fed no oilseeds, and a disproportionate increase in the birth weight of female calves was evident in cows fed oilseed. Total reproductive disorders tended to be greater in cows fed supplemental oilseed than those fed no oilseed (42 vs. 23%). Furthermore, cows fed sunflower seed had greater incidences of dystocia (35 vs. 18%) and total health disorders (52 vs. 32%) than those fed canola seed. Added oilseed and type of oilseed did not affect uterine inflammation at 25 ± 1 d postpartum. Oilseed supplementation did not alter the intervals from calving to establishment of the first dominant follicle, preovulatory-size follicle, and ovulation, nor did it affect fertility (conception rate to first artificial insemination and proportion of pregnant cows by 150 d after calving). In summary, prepartum oilseed supplementation (6.2 to 7.4% ether extract, % of dietary dry matter) decreased DMI during the entire experimental period (pre- and postpartum), decreased milk yield during early lactation in multiparous cows, and increased calf birth weight with no significant improvement in ovarian function and reproductive performance. [ABSTRACT FROM AUTHOR]

Our objective was to evaluate the effects of dietary starch content, altered by partial substitution of dietary grain with wheat dried distillers grain with solubles (DDGS), on the interval from calving to first ovulation, concentrations of hormones and metabolites in plasma and follicular fluid, and granulosa cell gene expression in preovulatory follicles. Sixty lactating dairy cows were assigned to 1 of 2 diets from calving until 84 d post-partum. Diets were formulated to contain either 17.3% rolled barley grain (29.2% starch) or 17.2% wheat DDGS (19.1% starch), with 43.0% barley silage and 21.6% rolled corn grain as the other major ingredients (dry matter basis). Transrectal ultrasonography was performed twice weekly to monitor ovarian dynamics from 7 ± 2 d postpartum until ovulation or until 56 d in milk, whichever occurred earlier. Plasma concentrations of insulin and insulin-like growth factor-1 (IGF-1) were determined in all 60 cows, and that of glucose, fatty acids, and urea in a subset of 24 cows, representing those in which the first ovulation occurred spontaneously within 5 wk postpartum. Estradiol (proestrus) and progesterone (12 d postovulation) in plasma were also measured. Concentrations of insulin, IGF-1, glucose, fatty acids, and urea were determined in follicular fluid (wk 9), and the expression of LH receptor, estrogen receptor β, cytochrome P450 aromatase, and plasma type glutathione peroxidase genes measured in granulosa cells obtained from the preovulatory follicles at wk 9 postpartum in the subset of 24 cows. Diets did not alter the interval from calving to first ovulation (32.3 ± 2.5 d), but a significantly lower proportion of cows on the DDGS diet (20%) ovulated multiple (≥2) follicles at the first ovulation than those on the barley grain diet (40%). The incidence of multiple ovulations tended to be lower at first insemination (10 vs. 21% for cows fed DDGS and barley grain diets, respectively). Mean plasma concentration of insulin was higher in cows fed the barley grain diet (2.5 vs 1.6 IU/mL), and a diet by time interaction was noted, with cows on the barley grain ration having higher insulin from wk 6 to 12 postpartum; however, mean plasma IGF-1 concentration did not differ between dietary groups. In the subsets, mean plasma concentrations of metabolites or estradiol and progesterone were not affected by diet, parity, or diet by parity interactions. Cows on the DDGS diet had lower concentrations of IGF-I (69 vs. 108 ng/mL) and higher fatty acids (222 vs. 149 mEq/L) in the follicular fluid obtained from preovulatory follicles. Diet, parity, and diet by parity interactions did not affect the concentrations of insulin, glucose, urea, estradiol, and progesterone in follicular fluid. Diets did not alter the expression profiles of LHr, estrogen receptor β, CYP19, and GPx3 genes in granulosa cells. In summary, diets did not affect the interval from calving to first ovulation or granulosa cell gene expression. However, reducing dietary starch content by a partial replacement of dietary grain with wheat DDGS increased fatty acids in follicular fluid and reduced the concentrations of insulin in plasma, IGF-1 in follicular fluid, and the incidence of multiple ovulations. [ABSTRACT FROM AUTHOR]

Una introducción se presenta en el cual los editores discuten varios informes en el tema sobre temas como el desarrollo folicular y la fertilidad en el ganado, el papel de las proteínas de unión al factor de crecimiento similar a la insulina en el ganado, y el desarrollo folicular durante el período posparto en vacas.

A protocol for presynchronization of ovarian status with 2 injections of PGF2α given 14 d apart, with the last PGF2α injection given 12 or 14 d before Ovsynch increases pregnancy per artificial insemination (P/AI) in dairy cows. We determined the efficacy of reducing the interval from the last PGF2α injection (500 μg of cloprostenol) of presynchronization to initiation of Ovsynch on response to treatment and P/AI. Lactating dairy cows were assigned to an Ovsynch protocol, with the initial injection of GnRH given either 9 (PRE-9; n = 135) or 12 d (PRE-12; n = 135) after the second PGF2α injection of presynchronization. The Ovsynch protocol consisted of 2 injections of 100 μg of GnRH given 9 d apart and 1 injection of PGF2α given 7 d after the initial GnRH injection, and cows were subjected to timed artificial insemination (TAI; 70 ± 3.5 DIM) approximately 16 h after the second GnRH injection. Body condition score (1-5 scale) was recorded at TAI. Blood samples were taken for progesterone determination at the PGF2α injection of Ovsynch, at TAI, and at 11 d after TAI. Ultrasonographic examinations were done in all cows at the second PGF2α injection of presynchronization, initial GnRH injection, PGF2α injection of Ovsynch, at TAI, and 24 h after TAI for cyclicity status and ovarian responses to treatments, and at 32 and 60 d after TAI for confirmation of pregnancy. Overall, 29 cows (10.7%) were determined acyclic or cystic and excluded from the study. The percentage of cows responding to initial GnRH injection (62.2 vs. 61.5%) did not differ between PRE-9 and PRE-12 but more cows in the PRE-9 group failed to respond to PGF2α treatment of Ovsynch compared with PRE-12 (22.7 vs. 10.7%). Body condition score at TAI (2.9 ± 0.02) and mean ovulatory follicle diameter (16.4 ± 0.2 mm) were not different between treatments. Overall P/ AI at 32 d was reduced in PRE-9 (33.6%) compared with PRE-12 (44.3%) but pregnancy losses (5.0 vs. 3.7%) did not differ between treatments. Primiparous cows in the PRE-12 group had higher mean progesterone concentration 11 d after TAI and greater P/AI 32 after TAI than primiparous cows in the PRE-9 group (6.4 ± 0.5 vs. 4.6 ± 0.5 ng/mL and 55.8 vs. 30.0%, respectively). In conclusion, reducing the interval from the last PGF2α injection of the presynchronization treatment to initiation of Ovsynch (from 12 to 9 d) did not affect ovulatory response to initial GnRH injection but reduced response to PGF2α injection of Ovsynch and P/AI at 32 and 60 d after TAI. The reduction in P/AI was particularly evident in primiparous cows of the PRE-9 group. [ABSTRACT FROM AUTHOR]

There is concern in the beef industry that selecting bulls for feed efficiency based on residual feed intake (RFI) may have a negative impact on bull repro-ductive performance and fertility. Here we investigated the impact of selection of bulls for low RFI on breeding soundness evaluation (BSE), reproductive performance, and fertility of bulls under natural service in multisire mating groups on pasture. Of the 412 RFI-tested bulls available, 98 (23.8%) were culled for performance, type, temperament, or other reasons, and 88 (21.4%) were culled for failing BSE, for an overall cull rate of 45.1%. From among the 314 bulls subjected to BSE, 32 (10.2%), 20 (6.4%), and 36 (11.4%) were culled for poor feet and legs, scrotal circumference, and semen quality, respectively. The BSE traits were not different (P > 0.10) between bulls categorized as either inefficient (+RFI) or efficient (-RFI), but the proportion of bulls that failed to meet the 60% minimum sperm motility requirement tended (P = 0.07) to be greater in the -RFI group than in the +RFI group (10.2% vs. 4.4%, respectively). In a subpopulation of 115 bulls, individual progressive spenn motility was greater {P < 0.05) in +RFI (85%) than -RFI (80%) bulls. A multisire natural mating experiment was conducted during 2 consecutive breeding seasons (2006 to 2007 and 2007 to 2008) using 18 +RFI and 18 -RFI bulls. The overall calving rate (calves born/cows exposed) was 72.9%. Mean number of progeny per sire was significantly greater (P <0.01) in -RFI bulls (18.3) than in +RFI bulls (11.8). Selection for feed efficiency based on RFI appears to have no detrimental impact on reproductive performance and fertility in beef bulls bred in multisire groups on pasture. However, the decreased sperm motility and the greater number of progeny per sire associated with -RFI status need further investigation. [ABSTRACT FROM AUTHOR]

The objective of the study was to examine whether residual feed intake (RFI) reranking exists between 2 consecutive periods in replacement heifers fed the same diet. The study collected feed intake and BW data from 190 crossbred heifers over a 3-yr period (61 in 2007, 68 in 2008, and 61 in 2009) during the winter-spring season. The heifers were fed a roughage-based diet (90% barley silage and 10% rolled barley grain) throughout the feeding trial, which was broken down into 2 feeding periods with ADG of 0.94 and 0.90 kg∙d-1 in the first and second periods, respectively. The RFI was calculated for the entire period using different models, which included ADG, mid-metabolic BW, body composition, and feeding activity. Gain:feed ratio and Kleiber ratio were also calculated. Rank correlations among the RFI calculated from different models were obtained, as well as rank correlations between the 2 feeding periods for the feed efficiency measures. Including body composition and feeding activity only improved the R2 by 1 to 5%. The rank correlations among the different models were high (90 to 95%) for RFI calculated for the entire feeding period. However, the RFI calculated within the second feeding period had greater rank correlation than the RFI calculated from the entire feeding period. Compared with G:F and Kleiber ratio, RFI gave lesser reranking between periods 1 and 2. About 49% of the heifers maintained their RFI class, whereas 51% of the heifers had a different RFI class in period 2. Furthermore, 41% of the heifers changed their RFI in period 2 by <0.5 SD, whereas the rest of the heifers changed by ⩾0.5 SD. These results indicate that reranking exists in heifers despite receiving the same diet in the 2 feeding periods and that the reranking may be more serious in heifers (28%) with extreme RFI performances in each period. [ABSTRACT FROM AUTHOR]

The objective of this study was to examine the effects of dietary starch source and content in the immediate postpartum period on plasma metabolites and hormones and ovarian follicular development. One of 3 diets was fed in a randomized block design to 40 cows from calving until 70 d in milk. The diets contained 45% alfalfa silage (AS), 45% barley silage (BS), or 41% barley silage and 4% supplemental starch (SS) on a dry matter basis. All diets contained 45% barley-based concentrate and 10% alfalfa hay. Resulting starch levels were 25.2, 23.3, and 26.7% for AS, BS, and SS, respectively. Body condition was scored every other week and dry matter intake and milk yield were recorded daily. Milk samples were obtained weekly and blood samples were taken at calving and then every other week to determine concentrations of glucose, insulin, insulin-like growth factor-1, β-hydroxybutyrate, and nonesterified fatty acids. Transrectal ultrasonography was performed twice per week from 7 d after calving until first ovulation or 62 d in milk in all cows. For a subset of 7 AS-, 8 BS-, and 9 SS-fed cows, a complete estrous cycle was monitored for ovarian dynamics, and blood samples were collected every second day for progesterone and estradiol. Luteinizing hormone pulsatility was also determined (5 cows/treatment) approximately 15 d postcalving. Treatment had no effect on body condition score, dry matter intake, blood metabolites, milk yield, or milk fat and protein contents, but BS-fed cows had significantly higher levels of milk urea nitrogen compared with SS cows. Cows fed SS (31 d) tended to have a shorter interval from calving to first ovulation than cows fed AS (43 d) or BS (38 d). The incidence of double first ovulations was higher in cows fed SS (46%) compared with those fed BS (0%). Treatment had no effect on LH pulse frequency or amplitude, ovarian dynamics, or progesterone and estradiol concentrations during the observed estrous cycle. Energy balance did not differ among cows fed the 3 diets. Overall, dietary starch source and concentration had little effect on productivity or metabolic status of postpartum cows. [ABSTRACT FROM AUTHOR]

The objective of this study was to determine the effects of feed restriction and source of dietary fatty acids during the close-up dry period on postcalving reproductive performance of dairy cattle. Thirty-four days before expected calving, pregnant Holstein cows (n = 72; parity 1 to 5) were randomly assigned to 1 of 6 treatments. Treatments were ad libitum (AL) or 24% feed restriction (FR) in combination with 1 of 3 oilseed supplements at 8% of diet dry matter: canola, linola, or flax to enrich the rations with oleic, linoleic, or linolenic fatty acids, respectively. After calving, cows were fed a common lactation diet that contained no oilseeds. Measurements of uterus, corpus luteum, and follicles were obtained by ultrasonography twice weekly from 7 ± 1 d after calving until the first ovulation. Cows (n = 66) were subjected to timed artificial insemination (TAI), and pregnancy was determined 32 d later. Feed-restricted cows had lower dry matter intake and lost more body weight prepartum. Energy balance (Mcal/d) was negative in FR cows prepartum but they had a less severe negative energy balance postpartum. The dietary source of fatty acid did not affect energy balance. Cows fed AL had a higher incidence of uterine infections (10/37 vs. 2/35) but tended to have fewer ovarian cysts (2/37 vs. 7/35) than FR cows. Mean (±SE) interval from calving to uterine involution did not differ among dietary treatments (26.8 ± 1.8 d). Interval from calving to first ovulation was longer in cows fed canola than in those fed either linola or flax (34.7 ± 3.1 vs. 23.7 ± 3.2 and 21.0 ± 3.1 d, respectively). A greater percentage of cows fed AL conceived to the first TAI (47.1 vs. 18.8) and tended to have fewer mean days open (157 ± 10.8 vs. 191 ± 10.1) than cows fed FR. In summary, FR cows had a lower incidence of uterine infections, but they were less fertile as reflected by a lower percent pregnancy to first TAI and increased days open. Cows fed diets enriched in linoleic or linolenic fatty acids had a lesser incidence of ovarian cysts and ovulated sooner with no effect on energy balance or fertility. [ABSTRACT FROM AUTHOR]

El artículo proporciona información sobre las aplicaciones y ventajas de la transferencia de embriones en bovinos. Empieza con la historia de la transferencia comercial de embriones en Norteamérica en los años setenta y sigue con la el progreso genético en la inseminación artificial. El artículo concluye con algunas consideraciones prácticas sobre la salud de las vacas donantes y las dosis de hormona folículo estimulante.

This study compared reproductive outcomes among two protocols for synchronization of ovulation that provide for a lengthened proestrus with the conventional oestradiol‐based protocol currently used for timed‐AI (TAI). Holstein heifers (13–15 months) at one location were assigned randomly to one of three TAI protocols. Heifers (n = 150) in the 7‐day oestradiol benzoate (EB) group received a progesterone device (Cue‐Mate) and 2 mg EB on Day 0; 500 μg of cloprostenol (PGF) and Cue‐Mate removal on Day 7; 1 mg of EB on Day 8 and TAI on Day 9 (54 h after Cue‐Mate removal). Heifers (n = 150) in the 5‐day CO‐Synch (CO) group received a Cue‐Mate and 100 μg of gonadotropin‐releasing hormone (GnRH) on Day 2; Cue‐Mate removal and PGF (twice, 12 h apart) on Day 7; and GnRH along with TAI on Day 10 (72 h after Cue‐Mate removal). Heifers (n = 150) in the J‐Synch (JS) group received a Cue‐Mate and 2 mg of EB on Day 1; PGF and Cue‐Mate removal on Day 7; GnRH and TAI on Day 10 (72 h after Cue‐Mate removal). Heifers were inseminated by one technician with frozen–thawed conventional semen from one of four commercially available sires. Plasma progesterone (P4) concentrations (ng/mL) were determined at Cue‐Mate removal and TAI. Ovarian ultrasonography was done in a subset of 217 heifers at the initiation of protocols, at Cue‐Mate removal; TAI; and 7 days after TAI. Approximately, 28 and 50 days after TAI pregnancy status was determined by ultrasonography. Mean (±SEM) plasma P4 concentration at Cue‐Mate removal was greater (p <.01) in CO (6.02 ± 0.2) and JS (6.51 ± 0.2) compared to EB heifers (4.53 ± 0.2). Mean (±SEM) plasma P4 concentration at TAI was lowest in the JS (0.28 ± 0.05), intermediate in CO (0.46 ± 0.02), and greatest in EB heifers (0.66 ± 0.05, p <.01). The diameter of the ovulatory follicle (mean ± SEM) was the smallest in the JS group compared to that in the CO and EB groups (15.8 ± 0.5; 13.9 ± 0.5; and 12.7 ± 0.5 mm for EB, CO and JS, respectively). More (p <.01) heifers in the JS group had their oestrous cycle synchronized (50.0, 78.8 and 82.4% for EB, CO and JS groups), and were pregnant at 28 (40.3, 51.3 and 63.3% for EB, CO and JS groups) and 50 days after TAI (32.6, 46.0 and 60.0% for EB, CO and JS groups). In summary, heifers subjected to the J‐Synch TAI protocol had lower P4 at TAI, and better overall response to hormonal treatments, which resulted in increased P/AI at 28 and 50 days after TAI compared to those heifers subjected to either a 7‐day EB protocol or a 5‐day CO‐synch protocol. [ABSTRACT FROM AUTHOR]

We investigate the photometric properties of 35 well-observed asteroids using dense ground-based and sparse ATLAS survey data. Focusing on two-colour photometric phase functions, derived using inverse methods, we explore the wavelength dependence. Our study reveals distinct (G 1, G 2) domains for cyan and orange filters, especially among some S-complex asteroids. For other asteroids, substantial uncertainties prevail, or their distributions of phase curve parameters overlap, precluding definitive conclusions on wavelength dependence. Notably, for S-complex objects, the effect appears systematic, characterized by lower G 2 values in the cyan filter. The effect can be explained by considering the known geometric albedo correlation: higher albedo corresponds to flatter, whereas lower albedo corresponds to steeper phase curves. In the case of equal albedo, asteroids with red spectral slopes have a more pronounced opposition effect in red and asteroids with blue spectral slopes in blue filters. We explore the variation of slope differences of orange and cyan phase curves with phase angle. For most asteroids, the largest nominal variation is observed at phase angles < 10°. This suggests that the phase colouring for the orange–cyan colour slope is more pronounced at small phase angles. Through meticulous analysis of the opposition effect amplitudes, we also pinpoint preferred rotational pole solutions. We identify inconsistencies between phase curve parameters and spectral types in specific cases. The shape- and geometry-corrected phase curves signify an important advancement in studying asteroid photometric behaviour and may offer a deeper understanding of surface and regolith properties previously obscured by these effects, such as surface roughness. [ABSTRACT FROM AUTHOR]

The objective was to compare pregnancy rates in nulliparous Holstein heifers given PGF2α 24 h before, or concurrent with, removal of an intravaginal progesterone- releasing (CIDR) insert in 3 timed artificial insemination (TAI) protocols. Heifers (from 2 herds) were assigned randomly, over 11 mo, to 1 of 3 modified Ovsynch protocols. On d 0 (without reference to the stage of the estrous cycle), all heifers were given 100 µg of GnRH i.m. and a CIDR insert (containing 1.9 g of progesterone). In the PG-7/P4-8 protocol (n = 99), PGF2α was given on d 7, and CIDR inserts were removed on d 8. In the PG-7/P4-7 (n = 98) and PG-8/P4-8 (n = 102) protocols, PGF2α administration and CIDR removal occurred concurrently, on d 7 or 8, respectively. In all 3 protocols, a second GnRH treatment (100 µg) was given 48 h after PGF2α, with TAI 16 to 20 h later. Blood samples were collected (subset of 124 heifers) on d 0, 7, 10 or 11 (i.e., at TAI), and 17. Pregnancy rates (32 d after TAI) for protocols PG-7/P4-8, PG-7/P4-7, and PG-8/P4-8 were 61.8, 55.6, and 54.1%, respectively. Pregnancy rate was higher when synchronization was initiated during diestrus than when initiated at other stages (57.0 versus 34.8%). Although pregnancy rates were not affected by season, there was an interaction between protocol and season; pregnancy rates were significantly lower in summer in heifers subjected to PG-7/P4-7 and PG-8/P4-8, but season did not affect pregnancy rates in heifers subjected to PG-7/P4-8. In summary, giving PGF2α 24 h before CIDR removal, followed by TAI (PG-7/P4-8 protocol), resulted in consistent pregnancy rates, regardless of season, relative to protocols involving PGF2α treatment concurrent with CIDR removal. [ABSTRACT FROM AUTHOR]

Context. Observations at the radio continuum band below the gigahertz band are key when the nature and properties of nonthermal sources are investigated because their radio radiation is strongest at these frequencies. The low radio frequency range is therefore the best to spot possible counterparts to very high-energy (VHE) sources: relativistic particles of the same population are likely to be involved in radio and high-energy radiation processes. Some of these counterparts to VHE sources can be stellar sources. Aims. The Cygnus region in the northern sky is one of the richest in this type of sources that are potential counterparts to VHE sources. We surveyed the central ∼15 sq deg of the Cygnus constellation at the 325 and 610 MHz bands with angular resolutions and sensitivities of 10″ and 6″, and 0.5 and 0.2 mJy beam−1, respectively. Methods. The data were collected during 172 h in 2013–2017, using the Giant Metrewave Radio Telescope with 32 MHz bandwidth, and were calibrated using the SPAM routines. The source extraction was carried out with the PyBDSF tool, followed by verification through visual inspection of every putative catalog candidate source in order to determine its reliability. Results. In this first paper we present the catalog of sources, consisting of 1048 sources at 325 MHz and 2796 sources at 610 MHz. By cross-matching the sources from both frequencies with the objects of the SIMBAD database, we found possible counterparts for 143 of them. Most of the sources from the 325-MHz catalog (993) were detected at the 610 MHz band, and their spectral index α was computed adopting S(ν) ∝ να. The maximum of the spectral index distribution is at α = −1, which is characteristic of nonthermal emitters and might indicate an extragalactic population. [ABSTRACT FROM AUTHOR]

Anogenital distance (AGD) serves as a marker for prenatal androgenization, reproductive development, and fertility in humans and rodents. The primary objectives of this observational study in lactating dairy cows were to (1) characterize the distribution and variability of AGD, (2) determine the relationship among AGD and potential postnatal AGD determinants of age and height, and (3) evaluate the associations between AGD and pregnancy to first artificial insemination (P/ AI) and cumulative pregnancy by 250 d in milk (DIM) within parity groups (first, second, and third+ parities). The secondary objective was to evaluate the association between AGD and testosterone concentrations. The AGD (mm), age (yr), and height at hip (cm) at the time of AGD determination, and aforesaid reproductive outcomes were determined in 921 Holstein cows (first, second, and third+ parity; n = 360, 256, and 305, respectively). Plasma concentrations of testosterone were determined in a subset of 93 cows. Overall, AGD had a normal distribution and high variability [mean (±standard deviation); 131.0 ± 12.2 mm], was weakly associated with cow age and height (coefficient of determination = 0.09 and 0.04, respectively), and had an inverse relationship with P/AI in first- and second-parity cows, but not in third+ parity cows. For every 1 mm increase in AGD, the odds of P/AI decreased by 3.4 and 2.4% for first- and second-parity cows, respectively. The optimal AGD threshold to predict probability of P/AI was 127.1 mm for both first- (sensitivity: 66.4; specificity: 56.6%) and second-parity cows (sensitivity: 46.0; specificity: 70.4%). Accordingly, first- and second-parity cows were categorized into either short or long AGD (≤ or >127.1 mm), and associations with reproductive outcomes were evaluated. First-parity cows with long AGD had lower P/AI (30.9 vs. 53.6%) and decreased likelihood (hazard ratio: 0.68) of pregnancy by 250 DIM than those with short AGD. Similarly, secondparity cows with long AGD had reduced P/AI (28.3 vs. 44.4%) and a tendency for decreased likelihood (hazard ratio: 0.76) of pregnancy by 250 DIM than in cows with short AGD. The association between AGD and testosterone was weak and nonsignificant. In summary, AGD in Holstein cows was normally distributed, highly variable, and weakly associated with age and height. Besides, AGD had an inverse relationship with P/AI and cumulative pregnancy by 250 DIM in first- and second-parity cows; however, such a relationship was not evident in older (third+ parity) cows. [ABSTRACT FROM AUTHOR]

The primary objective was to determine the variability and repeatability of GnRH-induced LH responses. The secondary objective was to evaluate the associations among plasma LH, FSH, estradiol (E2), and progesterone (P4) concentrations. One hundred lactating Holstein cows (35 primiparous, 65 multiparous) were initially subjected to a presynchronization protocol (d 0, PGF2α; d 3, GnRH) followed 7 d later by Ovsynch (d 10, GnRH; d 17, PGF2α; 56 h later, GnRH) and timed artificial insemination 16 h after the last GnRH. Blood samples were collected immediately before the GnRH injection of presynchronization and the second GnRH of Ovsynch to determine plasma concentrations of LH, FSH, and P4. A second blood sample was collected 2 h after each of the above GnRH injections to determine GnRH-induced LH and FSH concentrations. Plasma concentrations of E2 were also determined in samples collected immediately before the second GnRH of Ovsynch. Cows that (1) had higher LH concentrations at 0 h than at 2 h after GnRH, (2) showed an ongoing spontaneous LH surge, (3) did not respond to GnRH, and (4) had P4 ≥ 0.5 ng/mL at GnRH of presynchronization and the second GnRH of Ovsynch were excluded from the analysis. The variability (coefficient of variation) and repeatability [between animal variance/(within animal variance + between animal variance)] of GnRH-induced LH response were determined from samples collected 2 h after the GnRH of presynchronization and the second GnRH of Ovsynch. The associations among plasma LH, FSH, E2, and P4 were determined at the second GnRH of Ovsynch. Mean (±SEM) LH concentrations before GnRH were 0.5 ± 0.04 and 0.6 ± 0.03 ng/mL, whereas mean LH concentrations 2 h after GnRH were 9.8 ± 1.0 and 12.1 ± 0.8 ng/mL at GnRH of presynchronization and the second GnRH of Ovsynch, respectively. The variability of GnRH-induced LH was 76.1 and 52.1% at GnRH of presynchronization and the second GnRH of Ovsynch, respectively. The repeatability estimate for GnRH-induced LH concentration between GnRH of presynchronization and Ovsynch assessments was 0.10. Plasma concentrations of LH were positively associated with FSH and E2 (r = 0.61 and 0.30, respectively) and negatively associated with P4 (r = -0.46) at the second GnRH of Ovsynch. In summary, GnRH-induced LH responses were highly variable and unrepeatable, and LH concentrations were positively associated with FSH and E2 and negatively associated with P4. [ABSTRACT FROM AUTHOR]

Pregnancy per AI (P/AI) following the use of 1 of 2 timed AI (TAI) protocols and 2 different intervals between TAI and resynchronization were compared in heifers that were inseminated with either conventional or sex-sorted semen. Holstein heifers (n = 317; 527 inseminations) were submitted to a 5-d Cosynch protocol with (+) or without (-) GnRH at the time of controlled internal drug release (CIDR) insertion on d 0, CIDR removal and a single PGF2α treatment on d 5, and TAI plus GnRH on d 8 (72 h later). Visual estrus detection (ED) was conducted on d 6 in the afternoon and d 7 in the morning and heifers observed in estrus were artificially inseminated on d 7 in the afternoon. Heifers were alternately assigned conventional or sex-sorted semen. Pregnancy was diagnosed by ultrasound 27 and 42 d after AI, and heifers diagnosed as nonpregnant were resynchronized, up to 3 times, starting on d 27 or 34 to provide an interbreeding interval of 35 or 42 d. Overall, TAI protocol had no effect on P/AI at 27 or 42 d after artificial insemination or on pregnancy loss, but P/AI following the first service tended to be higher in the -GnRH TAI group (66.3 vs. 56.8%). Pregnancy per AI at 27 d (61.9 vs. 55.5%) tended to differ between conventional and sex-sorted semen. Heifers artificially inseminated based on ED tended to have a greater P/AI (67.6 vs. 58.2%) and had decreased pregnancy loss (0.0 vs. 4.1%) than those submitted to TAI. A greater number of heifers in the -GnRH TAI protocol were artificially inseminated on ED than the +GnRH TAI protocol (21.5 vs. 13.7%). No difference in P/AI was observed between the 35- and 42-d interbreeding intervals; however, more heifers in the 42-d group were artificially inseminated based on ED than in the 35-d group (22.7 vs. 7.8%). A 5-d Cosynch+CIDR TAI protocol without the initial GnRH and with a single PGF2α at CIDR removal is an acceptable alternative to achieve high P/AI when either conventional or sex-sorted semen is used in Holstein heifers. Breeding heifers based on detected estrus increases labor, but has the potential to increase fertility. [ABSTRACT FROM AUTHOR]

The objective of this study was to assess the efficacy of a single injection of Prostaglandin F2α (PGF2α) combined with or without GnRH before initiation of a Heatsynch protocol (GnRH-7d- PGF2α-2d-EB-1d-AI) on ovarian response and fertility in lactating Holstein cows. A total of 252 cows with a corpus luteum (CL; ≥10 mm) were assigned to one of three synchronization groups; 1 - Control (n=84), cows received two injections of PGF2α 14 days apart; 2 - Heatsynch with GnRH (PGH, n=88) the cows received PGF2α followed by GnRH four days later and then a Heatsynch protocol was initiated eight days after GnRH treatment; and 3 - Heatsynch without GnRH (PH, n=80) cows received a single injection of PGF2α followed by a Heatsynch protocol initiated 12 days after PGF2α. Cows detected in estrus were inseminated approximately 12 h after onset of estrus. Ovarian response and pregnancy diagnosis were determined by ultrasonography during the synchronization protocol and 30 days after AI, respectively. Results showed the percentage of cows with plasma P4≤0.4 ng/mL at AI were greater in PGH group compared to other groups (90.3 vs. 75.0%, P=0.03). The percentage of cows that ovulated in response to the GnRH injection of Heatsynch was affected (P<0.01) by synchronization protocol, because PGH cows were more likely to ovulate (77.2%) than PH (56.0%) cows. The proportion of cows displaying estrus was greater in PGH (70.4%) and PH (72.5%) groups compared with control (47.0%, P=0.04). Percentage of cows pregnant at 30 days after AI was (P=0.01) affected by synchronization treatment and was greater in PGH (45.16%) compared to control (25.0%) and PH (34.48%) groups. In summary, a short presynchronization that includes an injection of PGF2α and four days later GnRH increased fertility in Holstein cows subjected to a Heatsynch protocol. The enhanced fertility was due to a greater ovulatory response to GnRH of Heatsynch, more adequate plasma P4 concentrations during follicle development and a greater luteal regression following PGF2α prior to AI. [ABSTRACT FROM AUTHOR]

The objectives of this study were to determine the effect of PGF2α treatment 14 d before the initiation of a G6G or G7G (PGF2α, 2 d, GnRH, 6 or 7 d, Ovsynch) protocol on ovarian response, synchronization protocol on ovarian response, progesterone (P4) concentration, pregnancy per AI (P/AI), and pregnancy loss in multiparous Holstein cows. Cows (3.6 ± 1.1 lactations and yielding >30 kg/d of milk) were randomly assigned to 1 of 4 timed artificial insemination (TAI) protocols: 1) G6G (n = 240), one injection of PGF2α, GnRH 2 d later and a 7-d Ovsynch protocol (GnRH, 7 d, PGF2α, 56 h, GnRH 16 h TAI) was initiated 6 d later; 2) PG6G (n = 250), PGF2α 14 d before the initiation of the G6G protocol; 3) G7G (n = 200), one injection of PGF2α, GnRH 2 d later, and a 7-d Ovsynch protocol initiated 7 d later; and (4) PG7G (n = 200), a PGF2α injection 14 d before the initiation of the G7G protocol. Blood samples from a subset of 269 cows were collected at the times of first and second GnRH, and PGF2α of the Ovsynch protocol to measure P4. Ultrasound examinations were performed to evaluate ovarian response to GnRH and PGF2α of Ovsynch, and to determine pregnancy status at 32 and 60 d after TAI. The proportion of cows with high (≥1 ng/mL) P4 at first GnRH of Ovsynch was greater for PG6G and PG7G compared with G6G and G7G groups (combined 79.7 vs. 59.3%). In addition, mean (±SEM) plasma P4 concentration (ng/mL) at PGF2α of Ovsynch was also greater in PG6G (6.5 ± 0.2) and PG7G (6.7 ± 0.3) compared with G6G (5.1 ± 0.2) and G7G (5.0 ± 0.2). Cows given PGF2α 14 d before initiating a G6G or a G7G TAI (PG6G and PG7G) tended to have a greater P/AI at 32 d compared with those cows not receiving PGF2α (G6G and G7G). However, P/AI at 60 d was greater in cows subjected to PG6G and PG7G protocols (31.1 vs. 39.2%), with a lower pregnancy loss between 32 and 60 d (11.65 vs. 19.7%). In summary, administration of PGF2α 14 d before initiating a G6G or a G7G TAI protocol increased P4 concentrations before artificial insemination and late embryonic/early fetal survival in multiparous Holstein cows. [ABSTRACT FROM AUTHOR]

The objective of the study was to compare the effect of 2 free serum chemically defined media, synthetic oviduct fluid medium (SOF) and optimized simple potassium medium (KSOM) on embryo development of in vitro-produced embryos. Bovine ovaries were collected from a local abattoir. Cumulus oocyte complexes (COC) were obtained by follicular aspiration of 3- to 6-mm follicles and in vitro-matured in TCM-199 for 24 h at 39°C, 5% CO2, and high humidity. After IVM, COC were co-incubated with sperm at a concentration of 1.5 × 106spermatozoa per mL in TALP medium for 18 h at 39°C, 5% CO2, and high humidity. Presumptive zygotes were randomly assigned to SOF (n= 312) or KSOM (n= 290) media, both supplemented with essential and nonessential amino acids plus 0.6% BSA. Zygotes were cultured at 39°C, 5% CO2, 5% O2, 90% N2, and high humidity for 9 days. Cleavage rate was evaluated on Day 2 (Day 0 = day of insemination) and the number of blastocysts and hatched blastocysts were recorded on Days 8 and 9. Expanded blastocysts from Day 8 (n= 45/per culture medium) were fixed and stained with bisbenzimide (Hoechst 33242), and the total number of embryonic nuclei was counted by fluorescence microscopy (Optiphot, Nikon, Tokyo, Japan). The study was conducted in 6 replicates for embryo development assessment and 3 replicates for embryonic nuclei counting. Data were analyzed using Fisher''s exact test and Student''s t-test. The percentage of presumptive zygotes to develop into 2 to 4 cells (22.4 v. 24.2%) or > 4 cells (47.7 v. 53.4%) did not differ (P> 0.05) between SOF and KSOM on Day 2, but total embryo cleavage ≥ 2 cells was greater (P< 0.05) in KSOM (76.8%) than in SOF (68.5%). However, the percentage of blastocysts developed on Day 8 and 9 was greater (P< 0.05) for zygotes cultured in SOF (22.1 and 25.6%) than for those cultured in KSOM (15.1 and 18.9%). Furthermore, the percentages of hatched blastocysts on Day 8 and 9 were also greater (P< 0.05) for zygotes cultured in SOF (18.8 and 36.2%) than for those cultured in KSOM (4.7 and 12.5%). In addition, expanded blastocysts developed in SOF had higher total mean number of embryonic nuclei than those developed in KSOM (P< 0.05; 128.8 ± 7.5 v. 84.3 ± 8.9, respectively). In conclusion, the use of SOF resulted in significantly greater percentage of blastocysts and hatched blastocysts compared with KSOM. Additionally, expanded blastocysts developed in SOF displayed a higher number of cells. This study was supported by Convenio Desempeño en Investigacion (2007-DGI-CDA-04), Universidad Catolica de Temuco and DiD-UAcH: S-2009-26. [ABSTRACT FROM AUTHOR]