1. The potential mediation of hypoxia-inducible factor-1α in heat shock protein 27 translocations, caspase-3 and calpain activities and yak meat tenderness during postmortem aging.
- Author
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Zhu, Xijin, Dingkao, Renqing, Sun, Nan, Han, Ling, and Yu, Qunli
- Subjects
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HEAT shock proteins , *CALPAIN , *YAK , *CASPASES , *CYTOCHROME c , *OXYGEN consumption - Abstract
The present study aimed to characterize the influence of hypoxia-inducible factor-1α on heat shock protein 27 and cytochrome c translocation, yak meat microstructure destruction, and endogenous enzymes activities, refining the understanding of the tenderization process after slaughter. Postmortem yak longissimus thoracis et lumborum muscles were incubated with 0.9% saline or hypoxia-inducible factor-1α stabilizer dimethyloxaloylglycine at 4 °C for 6, 12, 24, 72, and 120 h. Results showed that hypoxia-inducible factor-1α activation promoted heat shock protein 27 migration and cytochrome c release, facilitating (P < 0.05) caspase-3 activity by mediating the heat shock protein 27/caspase-3 interaction but did not exert (P > 0.05) significant effects on the calpain-1 activity. Additionally, hypoxia-inducible factor-1α activation contributed to the mitochondrial apoptosis cascade, leading to a higher (P < 0.01) apoptosis rate. Therefore, these observations indicate that hypoxia-inducible factor-1α affects caspase-3 activity and tenderness of postmortem muscle through distinct regulatory mechanisms, possibly, in part, with heat shock protein 27 and cytochrome c mediation. • Hypoxia-inducible factor-1α elevated caspase-3 activity via inhibiting heat shock protein 27-caspase-3 interactions level. • The calcium levels and calpain-1 activity were not significantly affected by hypoxia-inducible factor-1α stabilization. • Hypoxia-inducible factor-1α involvement in the intrinsic mitochondrial apoptosis pathway improves muscle tenderization. • Steaks following hypoxia-inducible factor-1α activation possessed enhanced postmortem tenderness. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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