1. Mircrofabricating double-sided polydimethylsiloxane (PDMS) artificial phylloplane for microbial food safety research.
- Author
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Dong, Mengyi, Kavannaugh, Melannie, Lee, Caroline, and Feng, Hao
- Subjects
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ESCHERICHIA coli , *FOOD safety , *FOOD research , *POLYDIMETHYLSILOXANE , *POLYETHYLENE glycol , *EDIBLE greens , *FOOD transportation - Abstract
[Display omitted] • Double-sided PDMS-PDMS-PEG leaves were fabricated for seven common leafy greens. • Epicuticular wax prevents wetting and E. coli O157:H7 attachment on leaf surface. • Wax also enhanced the retention of attached E. coli O157:H7 on leaf surface. • E. coli O157:H7 survived and grew on artificial leaves with nutrients supplied. • The artificial leaves behaved similarly to its natural leaves in sanitation. Leafy green surface microbiology studies often experience significant variations in results due to the heterogeneous nature of leaf surfaces. To provide a precise and controllable substitute, we microfabricated double-sided artificial leafy green phylloplanes using polydimethylsiloxane (PDMS) with a vinyl-terminated polyethylene glycol chain-based hydrophobicity modifier (PDMS-PEG) to modify PDMS hydrophobicity. We further tested the properties and applications of these artificial leaves, by examining the function of epicuticular wax, growth and survival of E. coli O157:H7 87–23 on the surface, and removal of attached E. coli cells via sanitation. The double-sided PDMS-PDMS-PEG leaves well-replicated their natural counterparts in macroscopic and microscopic structure, hydrophobicity, and E. coli O157:H7 87–23 attachment. After depositing natural epicuticular wax onto artificial leaves, the leaf surface wetting ability decreased, while E. coli O157:H7 87–23 surface retention increased. The artificial leaves supplied with lettuce lysate or bacterial growth media supported E. coli O157:H7 87–23 growth and survival similarly to those on natural leaves. In the sanitation test, the artificial lettuce leaves also displayed patterns similar to those of natural leaves regarding sanitizer efficiency. Overall, this study showcased the microfabrication and applications of double-sided PDMS-PDMS-PEG leaves as a replicable and controllable platform for future leafy green food safety studies. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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