1. Gene expression profile analysis of pig muscle in response to cold stress.
- Author
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Dong-jie, Zhang, Di, Liu, Liang, Wang, Wen-tao, Wang, Xin-miao, He, and Guo-wei, Yang
- Subjects
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LIVESTOCK & the environment , *PHYSIOLOGICAL effects of cold temperatures , *WILD boar , *EFFECT of temperature on swine , *MUSCULOSKELETAL system - Abstract
Low-temperature injury is one of the most significant causes of livestock damage worldwide. To identify genes that respond to cold stress, the transcriptome profile of the skeletal muscle of the Min pig (Sus scrofa) after cold stress was analysed using the deep sequencing technique. One group was raised in the normal environment (18 ± 2°C,n = 3) as the thermos-neutral group; another group was raised in the low-temperature environment (4 ± 2°C,n = 3) as the cold stress group. After 24 h, the gene expression profile of skeletal muscle was analysed. The results showed that the expression levels of 662 genes were significantly up-regulated, and those of 1058 genes were significantly down-regulated in the thermos-neutral and the cold stress groups (P < 0.01). It was found through Gene Ontology analysis that the significantly differently expressed genes were mainly distributed in the cytoplasm, mitochondrion, organelle envelope and ribosomal subunit. They enriched ribosome, fatty acid metabolism, RNA transport and metabolic pathways. It means that cold stress could significantly change the gene expression profile of pig muscle. [ABSTRACT FROM PUBLISHER]
- Published
- 2017
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