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The pathogenesis of white spot syndrome virus (WSSV) is largely unclear. In this study, we found that actin nucleation and clathrin-mediated endocytosis were recruited for internalization of WSSV into crayfish hematopoietic tissue (Hpt) cells. This internalization was followed by intracellular transport of the invading virions via endocytic vesicles and endosomes. After envelope fusion within endosomes, the penetrated nucleocapsids were transported along microtubules toward the periphery of the nuclear pores. Furthermore, the nuclear transporter CqImportin a1/b1, via binding of ARM repeat domain within CqImportin a1 to the nuclear localization sequences (NLSs) of viral cargoes and binding of CqImportin b1 to the nucleoporins CqNup35/62 with the action of CqRan for docking to nuclear pores, was hijacked for both targeting of the incoming nucleocapsids toward the nuclear pores and import of the expressed viral structural proteins containing NLS into the cell nucleus. Intriguingly, dysfunction of CqImportin a1/b1 resulted in significant accumulation of incoming nucleocapsids on the periphery of the Hpt cell nucleus, leading to substantially decreased introduction of the viral genome into the nucleus and remarkably reduced nuclear import of expressed viral structural proteins with NLS; both of these effects were accompanied by significantly inhibited viral propagation. Accordingly, the survival rate of crayfish post-WSSV challenge was significantly increased after dysfunction of CqImportin a1/b1, also showing significantly reduced viral propagation, and was induced either by gene silencing or by pharmacological blockade via dietary administration of ivermectin per os. Collectively, our findings improve our understanding of WSSV pathogenesis and support future antiviral designing against WSSV. IMPORTANCE As one of the largest animal DNA viruses, white spot syndrome virus (WSSV) has been causing severe economical loss in aquaculture due to the limited knowledge on WSSV pathogenesis for an antiviral strategy. We demonstrate that the actin cytoskeleton, endocytic vesicles, endosomes, and microtubules are hijacked for WSSV invasion; importantly, the nuclear transporter CqImportin a1/b1 together with CqRan were recruited, via binding of CqImportin b1 to the nucleoporins CqNup35/62, for both the nuclear pore targeting of the incoming nucleocapsids and the nuclear import of expressed viral structural proteins containing the nuclear localization sequences (NLSs). This is the first report that NLSs from both viral structure proteins and host factor are elaborately recruited together to facilitate WSSV infection. Our findings provide a novel explanation for WSSV pathogenesis involving systemic hijacking of host factors, which can be used for antiviral targeting against WSSV disease, such as the blockade of CqImportin a1/b1 with ivermectin. [ABSTRACT FROM AUTHOR]

Judicious use of lamps is of profound significance to improve the internal traffic safety of tunnels. This study evaluated the biological effects of spectrum on human visual and nonvisual under mesopic vision category. According to the difference of human eyes' response to the visual and nonvisual biological effects of different wavelengths of light radiation, a method of applying the mesopic visual spectral luminous efficiency curve to the assessment of nonvisual biological effects and blue light hazards is proposed. The results show that, in theory, under mesopic vision category, the spectrum with high correlation color temperature (CCT) and high color rendering index (CRI) has higher blue light content Z-value and has greater impact on human nonvisual effects and blue light radiation damage, but it can also provide higher human perception luminance. The visual effect detection experiment of driving in the interior zones of the tunnel is simulated in the laboratory, and the critical flicker frequency and reaction time are taken as physiological indexes. The results show that the spectrum with high CCT and high CRI corresponds to lower critical flicker frequency drop value and faster reaction time, which has a positive effect on improving driving safety. Therefore, this paper suggests that the LED with high CCT and high CRI (CCT = 6500 K, CRI2012 = 95) should be used in the interior zones of the tunnel with relatively short lighting section, and the CCT and CRI should be appropriately reduced in other road environments with long lighting section to reduce the impact of spectrum on nonvisual photobiology, so as to improve road traffic safety. [ABSTRACT FROM AUTHOR]

Titanium dioxide nanoparticles (nTiO2) have raised environmental concerns and display potential hazards to aquatic organisms and even humans. However, only a few reports tested the toxicity of nTiO2 to rotifers. In the present study, acute and chronic toxicity of nTiO2 to the freshwater rotifer Brachionus calyciflorus was investigated at different temperatures. At 25°C, the 24 and 48‐hr LC50 was 117.14 and 60.11 mg/L, respectively. At 15°C, 20°C, 25°C, and 30°C, exposure to nTiO2 significantly decreased life expectancy at birth, net reproductive rate, generation time, average lifespan, and/or intrinsic rate of population increase of B. calyciflorus (p <.05). High temperature enhanced the toxicity of nTiO2 to rotifers. The swimming linear speed of rotifers significantly increased (p <.05) in treatments with 200 µg/L nTiO2, compared with the control. In addition, treatments with 8 µg/L to 5 mg/L nTiO2 significantly increased superoxide dismutase activity (p <.05). Glutathione content and catalase activity increased significantly after exposure to 8 µg/L nTiO2 but decreased significantly in treatments with nTiO2 concentrations ranging from 40 µg/L to 5 mg/L (p <.05). There were no significant changes in malondialdehyde contents among nTiO2 treatments and control. Overall, the present study indicated that nTiO2 revealed high toxicity to rotifers, displaying high environmental risks to aquatic ecosystems. HIGHLIGHTS: 1.High temperature enhanced the toxicity of nTiO2 to rotifers.2.The 24 and 48‐hr LC50 of nTiO2 to B. calyciflorus was 117.4 and 60.11 mg/L, respectively.3.nTiO2 displayed high environmental risk to aquatic ecosystems even at the µg/L levels. [ABSTRACT FROM AUTHOR]

Binderless boards were produced from bamboo particles through a molding pressing process. The boards were investigated for the chemical and structural changes of lignin and their mechanical strengths to evaluate the mechanism of self-bonding. The structural transformations of milled wood lignin (MWL) obtained from raw bamboo and molding pressed bamboo boards were investigated by Fourier transform infrared (FT-IR), gel permeation chromatography (GPC), quantitative 13C-NMR spectra, two-dimensional heteronuclear single quantum coherence (2DHSQC) spectra, 31P-NMR, thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) techniques. Both molding press temperature and time affected the distribution and abundance of typical lignin linkages (β-O-4', β-β', and β-5'), as well as the S/G ratios of the lignin, demethoxylation, and the contents of attached molecules (PCE). In addition, a decrease in aliphatic OH and an increase in phenolic hydroxyl groups occurred in lignin as molding pressing proceeded. The optimal internal bonding strength (0.98 MPa) of the bamboo binderless board was obtained under the condition of 180 °C for 20 min. Although the lignin obtained under this condition was structurally similar to the raw MWL, the decreased molecular weight, increased phenolic hydroxyl groups, and observable glass-transition temperature (thermal softening of lignin) provide some evidence to explain the acceptable internal bonding strength. [ABSTRACT FROM AUTHOR]

To reuse waste wood bioresources and determine the factors required for the growth of Lentinus edodes, Quercus baronii wood bioconversion during the artificial cultivation of L. edodes was characterized by X-ray diffraction (XRD), TG, FT-IR, and TD-GC-MS. Mycelia were observed to grow in wood if cellulose was sufficiently degraded and wood extractives were adequately retained. L. edodes grew in wood if the extractives, cellulose, hemicellulose, and lignin maintained a stable quality ratio. Mycelium and L. edodes grew in samples with high cellulose crystallinity. FT-IR spectra showed that L. edodes grew as the intensity of absorbance associated with unconjugated C=O stretching decreased. TG curves suggested that the samples with lower weight loss were suitable for mycelium, but those with higher weight loss were suitable for L. edodes. TD-GC-MS indicated that the samples containing more phenol derivatives and less acetic acid were suitable for mycelium; the opposite trends were observed for L. edodes. [ABSTRACT FROM AUTHOR]

Autophagy involves the sequestration and lysosomal degradation of various cytoplasmic structures, including damaged organelles and invading microorganisms. Autophagy is not only an essential cell-intrinsic mechanism for protecting against internal and external stress conditions but is also key in the cellular response against microbes, in antigen processing for major histocompatibility complex (MHC) presentation, and in lymphocyte development, survival, and proliferation. In recent years, perturbations in autophagy have been implicated in a number of diseases, including autoimmune diseases, such as systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), and multiple sclerosis (MS). Immune thrombocytopenia (ITP) is a multifactorial disease characterized by autoimmune responses to self-platelet membrane proteins. Recently, our unpublished original data demonstrated aberrant expression of molecules in the autophagy pathway in ITP patients compared with controls, and we found a close correlation between the pathogenesis of ITP and the autophagy pathway. The potential of targeting the autophagy pathway in ITP as a novel therapeutic approach has been discussed. [ABSTRACT FROM AUTHOR]

To achieve high value-added utilization of lignin extracted from the biorefinery process in the wood composite industry, binderless particle boards were manufactured by bamboo materials combined with alkaline lignin (AL) in various proportions under various hot-pressing conditions. To elucidate the reactivity and chemical transformations of lignin macromolecules during the hot-pressing process, lignin samples were isolated from the corresponding boards and characterized by highperformance anion-exchange chromatography (HPAEC), gel permeation chromatography (GPC), quantitative 31P-NMR, and 2D-HSQC NMR. The best bonding strength (1.36 MPa) of the binderless particle boards was obtained under the conditions of 180 °C, pressure 5 MPa, and lignin/bamboo mass ratio 0.4. The molecular weight of the lignin samples decreased from 3260 to 1420 g/mol during hot-pressing. The NMR results showed that the contents of β-O-4' and β-β' linkages were reduced and β-5' linkages were increased as the hot-pressing temperature rose. Simultaneously, the percentage of G-type and H-type lignins as well as the content of phenolic OH increased. [ABSTRACT FROM AUTHOR]

Highlights: [•] pH had an effect on the microstructure and photochromic properties of composite films. [•] The intensity of absorbance was decreased with increase of pH after UV irradiated. [•] Responsiveness was related to microstructure’s change generated by different pH. [ABSTRACT FROM AUTHOR]

Abstract: A simple, rapid and improved method was developed to isolate high-purity baicalein and wogonin from Scutellariae radix. The method involves endogenous baicalinase-catalysed hydrolysis (EBCH), partition, automated low-pressure preparative chromatography (LPPC) and recrystallisation without multiple and tedious column chromatography. This process was optimised for large scale production of baicalein and wogonin with high yields, low costs and process automation. The transformation ratio of baicalin and wogonoside reached 98.21% and 96.60% after EBCH, leading to an increase of 5.41-fold in baicalein and 3.89-fold in wogonin, compared to a raw sample without hydrolysis. The purity of final products was more than 98% after one-step LPPC and recrystallisation. The experimental results show that EBCH–LPPC is an effective method for preparing high purity antioxidants. [Copyright &y& Elsevier]

Abstract: Baicalinase is an endogenous enzyme present in the roots of Scutellaria baicalensis that can hydrolyse baicalin and wogonoside into baicalein and wogonin directly, which have higher bioactivity and bioavailability than their glycosides. Baicalinase-catalysed hydrolysis with cavitation was examined as a highly efficient and green alternative method for large scale production of baicalein and wogonin. Effect of cavitation system on the transformation and extraction efficiency was investigated by using reaction kinetic and mass transfer kinetic models at three levels of temperature (20–50°C). Experimental results showed that cavitation had a significant effect (p <0.01) on the biocatalysis process; the transformation of baicalin and wogonoside reached 98.21% and 96.60% at 50°C for 60min, and the yields of baicalein and wogonin were about 4.47- and 2.86-fold higher than those of the raw sample without enzymatic hydrolysis, respectively. [Copyright &y& Elsevier]

Abstract: To enhance the extraction efficiency and reduce the energy consumption, an emerging technology named negative pressure cavitation extraction (NPCE) has been shown to be a feasible option for the extraction of bioactive compounds in agricultural crops and medicinal plants. Meanwhile, it can be applied at the pilot scale as a manufacturing process for edible and medicinal plants. Currently, NPCE was proposed for extraction of baicalin, wogonoside, baicalein and wogonin from Radix Scutellariae on the basis of a central composite design (CCD) and response surface methodology (RSM). With proper optimization (80 mesh of particle size, 40mL/g of liquid/solid ratio, 75% aqueous ethanol as extraction solvent, 60min extraction time and −0.07MPa vacuum degree), NPCE was observed to have good extraction efficiency compared with other conventional extraction methods. Furthermore, the antioxidant activities of crude extracts with different extraction methods were assessed by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay. Our results showed that NPCE extract possessed better antioxidant activity with IC50 value of 3.24μg/mL compared with the UAE, HRE and SE extracts with IC50 values of 7.85, 12.14 and 11.44μg/mL, respectively. [Copyright &y& Elsevier]

A novel zwitterionic imidazolium-based ionic liquid (IL) surfactant, 1-carboxymethyl-3-dodecylimidazolium inner salt, was synthesized. The molecule structure was confirmed by means of electrospray ionization mass spectrometry, H nuclear magnetic resonance and elemental analysis. The isoelectric point (pI) is 3.8 ± 0.1 at 35 ± 0.1 °C. The other important physicochemical parameters such as the critical micelle concentration (CMC), the surface tension at CMC (γ), the adsorption efficiency (p C), the surface pressure at CMC (Π), the maximum surface excess (Γ), the minimum molecular cross-sectional area ( A), the value of CMC/ C and the average number of aggregation ( N) were determined by surface tension and steady-state fluorescence probe methods, respectively. [ABSTRACT FROM AUTHOR]

This paper deals with the synthesis and self-aggregation of a hydroxyl-functionalized imidazolium-based ionic liquid (IL) surfactant, namely 1-hydroxyethyl-3-dodecylimidazolium chloride ([COHCim]Cl). The molecular structure was confirmed by means of electrospray ionization mass spectrometry (ESI-MS), H nuclear magnetic resonance (H NMR) and elemental analysis. Many important physicochemical parameters, such as the critical micelle concentration (CMC), the surface tension at CMC (γ), the adsorption efficiency (p C), the surface pressure at CMC (Π), the maximum surface excess (Γ), the minimum molecular cross-sectional area ( A), the value of CMC/ C, the average number of aggregation ( N) and the micellar microenvironment polarity were determined by surface tension-concentration curves, fluorescence spectra, and electrical conductivity. The phenomena of the second CMC, the concentration dependence of N, and the critical average aggregation number ( N) of imidazolium-based IL surfactants are reported for the first time in this paper. [ABSTRACT FROM AUTHOR]

Jussiaea repens L. (JRL) is an edible medicinal plant and is also used as a vegetable by the local people in southwestern China. The crude extract and its four fractions derived from JRL were evaluated for the 1,1-diphenyl-2-picrylhydrazyl radical-scavenging ability, hydroxyl radical-scavenging capacity and the potassium ferricyanide reduction property. The ethyl acetate-soluble fraction (EAF) and EAF6 (a subfraction derived from EAF) were the most valuable fraction and subfraction, respectively. Furthermore, bioactivity-guided chromatographic fractionation revealed that three pure compounds greatly contributed to the antioxidant activities. Qualitative and quantitative analyses of the major antioxidant constituents in the extract were systematically conducted by NMR, mass spectral analyses and RP-HPLC. The result demonstrated that rosmarinic acid (2.00 mg g−1 JRL dry weight) quercetin 3- O- β-d-glucopyranoside (9.88 mg g−1 JRL dry weight), and kaempferol 3- O- β-d-glucopyranoside (1.85 mg g−1 JRL dry weight) were the major antioxidative constituents in JRL. These compounds are reported for the first time from this plant. [ABSTRACT FROM AUTHOR]

Background: Evaluation of fluid responsiveness during veno-arterial extracorporeal membrane oxygenation (VA-ECMO) support is crucial. The aim of this study was to investigate whether changes in left ventricular outflow tract velocity–time integral (ΔVTI), induced by a Trendelenburg maneuver, could predict fluid responsiveness during VA-ECMO. Methods: This prospective study was conducted in patients with VA-ECMO support. The protocol included four sequential steps: (1) baseline-1, a supine position with a 15° upward bed angulation; (2) Trendelenburg maneuver, 15° downward bed angulation; (3) baseline-2, the same position as baseline-1, and (4) fluid challenge, administration of 500 mL gelatin over 15 min without postural change. Hemodynamic parameters were recorded at each step. Fluid responsiveness was defined as ΔVTI of 15% or more, after volume expansion. Results: From June 2018 to December 2019, 22 patients with VA-ECMO were included, and a total of 39 measurements were performed. Of these, 22 measurements (56%) met fluid responsiveness. The R2 of the linear regression was 0.76, between ΔVTIs induced by Trendelenburg maneuver and the fluid challenge. The area under the receiver operating characteristic curve of ΔVTI induced by Trendelenburg maneuver to predict fluid responsiveness was 0.93 [95% confidence interval (CI) 0.81–0.98], with a sensitivity of 82% (95% CI 60–95%), and specificity of 88% (95% CI 64–99%), at a best threshold of 10% (95% CI 6–12%). Conclusions: Changes in VTI induced by the Trendelenburg maneuver could effectively predict fluid responsiveness in VA-ECMO patients. Trial registration ClinicalTrials.gov, NCT 03553459 (the TEMPLE study). Registered on May 30, 2018 [ABSTRACT FROM AUTHOR]

As the most severely lethal viral pathogen for crustaceans in both brackish water and freshwater, white spot syndrome virus (WSSV) has a mechanism of infection that remains largely unknown, which profoundly limits the control of WSSV disease. By using a hematopoietic tissue (Hpt) stem cell culture from the red claw crayfish Cherax quadricarinatus suitable for WSSV propagation in vitro, the intracellular trafficking of live WSSV, in which the acidic-pH-dependent endosomal environment was a prerequisite for WSSV fusion, was determined for the first time via live-cell imaging. When the acidic pH within the endosome was alkalized by chemicals, the intracellular WSSV virions were detained in dysfunctional endosomes, resulting in appreciable blocking of the viral infection. Furthermore, disrupted valosincontaining protein (C. quadricarinatus VCP [CqVCP]) activity resulted in considerable aggregation of endocytic WSSV virions in the disordered endosomes, which subsequently recruited autophagosomes, likely by binding to CqGABARAP via CqVCP, to eliminate the aggregated virions within the dysfunctional endosomes. Importantly, both autophagic sorting and the degradation of intracellular WSSV virions were clearly enhanced in Hpt cells with increased autophagic activity, demonstrating that autophagy played a defensive role against WSSV infection. Intriguingly, most of the endocytic WSSV virions were directed to the endosomal delivery system facilitated by CqVCP activity so that they avoided autophagy degradation and successfully delivered the viral genome into Hpt cell nuclei, which was followed by the propagation of progeny virions. These findings will benefit anti-WSSV target design against the most severe viral disease currently affecting farmed crustaceans. [ABSTRACT FROM AUTHOR]

Objectives: To investigate the prognostic value of coronary CT angiography (CCTA) in heart failure patients with preserved ejection fraction (HFpEF). Methods: Between January 2009 and December 2013, 6497 participants (mean age 63 ± 9.4 [range 32–86] years; 4111 men) who underwent CCTA and echocardiography were prospectively included. Participants were divided into HFpEF group and without HFpEF group. The primary endpoint was major adverse cardiovascular events (MACEs), including cardiovascular mortality, nonfatal myocardial infarction (MI), or hospitalization for heart failure (HF). Results: Among those participants, 3096 were identified with HFpEF and 3401 were without HFpEF. Higher prevalence of coronary atherosclerosis was observed in HFpEF group than those without (78.3% vs. 64.9%, p < 0.001). During a median of 11.0 [IQR: 9.0–12.0] years follow-up, participants with HFpEF exhibit a heightened risk of MACEs in CAD-RADS = 0, 1–2, and ≥ 3 respectively (p < 0.001 for all). In the risk-adjusted hazard analysis among participants with HFpEF, CAD-RADS = 1–2 increased a 2.5-time risk for non-fatal MI (adjusted HR: 2.5, 95% CI: 1.5 to 4.3, p < 0.001), while CAD-RADS ≥ 3 conferred 3.9-fold and 3.1-fold higher risk for cardiovascular mortality (adjusted HR: 3.9, 95% CI: 2.2 to 7.1, p < 0.001) and hospitalization due to HF (adjusted HR: 3.1, 95% CI: 1.9 to 5.3, p < 0.001) with reference to CAD-RADS = 0 respectively. Conclusions: Coronary artery disease is common in participants with HFpEF and associated with MACEs. Among those participants, the presence of CAD-RADS = 1–2 increased the risk of nonfatal MI, while CAD-RADS ≥ 3 were correlated with cardiovascular mortality and hospitalization due to HF. Key Points: • Higher median of CACS and higher CAD-RADS categories were observed in the HFpEF group than those without (p < 0.001 for both). • Participants with HFpEF exhibit a heightened risk of MACEs in CAD-RADS = 0, 1–2, and ≥ 3 respectively (p < 0.001 for all). • In the risk-adjusted hazard analysis among participants with HFpEF, CAD-RADS =1–2 increased a 2.5-time risk for non-fatal MI (adjusted HR: 2.5, 95% CI: 1.5 to 4.3, p < 0.001) with reference to CAD-RADS = 0 respectively. [ABSTRACT FROM AUTHOR]

Different oleanolic acid (OA) oxime ester derivatives (3a-3t) were designed and synthesised to develop inhibitors against α-glucosidase and α-amylase. All the synthesised OA derivatives were evaluated against α-glucosidase and α-amylase in vitro. Among them, compound 3a showed the highest a-glucosidase inhibition with an IC50 of 0.35 µM, which was ~1900 times stronger than that of acarbose, meanwhile compound 3f exhibited the highest α-amylase inhibitory with an IC50 of 3.80 µM that was ~26 times higher than that of acarbose. The inhibition kinetic studies showed that the inhibitory mechanism of compounds 3a and 3f were reversible and mixed types towards α-glucosidase and α-amylase, respectively. Molecular docking studies analysed the inter- action between compound and two enzymes, respectively. Furthermore, cytotoxicity evaluation assay demonstrated a high level of safety profile of compounds 3a and 3f against 3T3-L1 and HepG2 cells. [ABSTRACT FROM AUTHOR]

Background: Nonalcoholic fatty liver disease (NAFLD) is a chronic metabolic disease that easily induces hepatitis, cirrhosis, and even liver cancer. The long-term use of NAFLD therapeutic drugs produces toxicity and drug resistance. Therefore, it is necessary to develop high efficiency and low-toxicity active ingredients to alleviate NAFLD. Objective: This study aimed to reveal the role and mechanism of a new functional food CMT in alleviating NAFLD. Results: In the ob/ob fatty liver mice models, the CMT extracts significantly inhibited the weight gain of the mice and reduced the accumulation of white fat. The anatomical and pathological results showed that CMT relieved fatty liver in mice and reduced excessive lipid deposition and inflammatory infiltration. Serological and liver biochemical indicators suggest that CMT reduced dyslipidemia and liver damage caused by fatty liver. CMT obviously activated the adenosine 5'-monophosphate-activated protein kinase (AMPK)/acetyl-coA carboxylase (ACC) and AMPK/fatty acid synthase (FAS) signaling pathways, promoted fat oxidation, and inhibited synthesis. Moreover, CMT regulated the expression of inflammatory factors to relieve hepatitis caused by NAFLD. Conclusion: The study explained the role and mechanism of CMT in alleviating NAFLD and suggested that the active ingredients of CMT might be beneficial in NAFLD therapy. [ABSTRACT FROM AUTHOR]

Aims: To determine psychological symptoms of patients with mild symptoms of coronavirus disease 2019 in China and to explore the influencing factors. Design: A cross‐sectional study. Methods: A convenience sample of 296 mild coronavirus disease 2019 patients were recruited from a Fangcang hospital in Wuhan, Hubei Province, from 3–5 March, 2020. Participants were assessed using a sociodemographic and clinical characteristics questionnaire, and Symptom Check List 90. The binary logistic regression was utilized to explore the influencing factors of psychological symptoms of patients with mild symptoms of coronavirus disease 2019. Results: In total, 296 of 299 patients with mild symptoms of coronavirus disease 2019 participated in the study (response rate: 99.0%). The findings revealed that 12.8% patients with mild symptoms have mental health problems; the most common psychological symptoms are phobic anxiety (58.4%), paranoid ideation (50.7%) and psychoticism (40.2%). Female patients [OR = 3.587, 95% CI (1.694–7.598)] and those having physical symptoms currently [OR = 2.813, 95% CI (1.210–6.539)] are at higher risk, while those in the middle duration of hospitalization [OR = 0.278, 95% CI (0.121–0.639)] protect against mental‐health problems. Conclusions: The minority of patients with mild symptoms of coronavirus disease 2019 were still suffering from psychological symptoms. Healthcare providers are recommended to pay particular attention to screening these high‐risk groups (women, those in the initial stages of hospitalization and those with physical symptoms currently) and implement targeted psychological care as required. Impact: This study found that most patients of coronavirus disease 2019 in Fangcang hospital exhibited normal mental health at par with the general Chinese norm and the minority of them were suffering from psychological symptoms. The findings can provide a reference for healthcare providers to screen high‐risk psychological symptoms groups and implement targeted psychological intervention for patients with coronavirus disease 2019. [ABSTRACT FROM AUTHOR]

Background: MicroRNA-612 (miR-612) has been proven to suppress EMT, stemness, and tumor metastasis of hepatocellular carcinoma (HCC) via PI3K/AKT2 and Sp1/Nanog signaling. However, its biological roles on HCC progression are far from elucidated. Methods: We found direct downstream target of miR-612, hadha by RNA immunoprecipitation and sequencing. To explore its biological characteristic, potential molecular mechanism, and clinical relevance in HCC patients, we performed several in-vitro and in-vivo models, as well as human tissue chip. Results: Ectopic expression of miR-612 could partially reverse the level of HADHA, then suppress function of pseudopods, and diminish metastatic and invasive potential of HCC by lipid reprogramming. In detail, miR-612 might reduce invadopodia formation via HADHA-mediated cell membrane cholesterol alteration and accompanied with the inhibition of Wnt/β-catenin regulated EMT occurrence. Our results showed that the maximum oxygen consumption rates (OCR) of HCCLM3miR-612-OE and HCCLM3hadha-KD cells were decreased nearly by 40% and 60% of their counterparts (p < 0.05). The levels of acetyl CoA were significantly decreased, about 1/3 (p > 0.05) or 1/2 (p < 0.05) of their controls, in exogenous miR-612 or hadha-shRNA transfected HCCLM3 cell lines. Besides, overexpression of hadha cell lines had a high expression level of total cholesterol, especially 27-hydroxycholesterol (p < 0.005). SREBP2 protein expression level as well as its downstream targets, HMGCS1, HMGCR, MVD, SQLE were all deregulated by HADHA. Meanwhile, the ATP levels were reduced to 1/2 and 1/4 in HCCLM3miR-612-OE (p < 0.05) and HCCLM3hadha-KD (p < 0.01) respectively. Moreover, patients with low miR-612 levels and high HADHA levels had a poor prognosis with shorter overall survival. Conclusion: miR-612 can suppress the formation of invadopodia, EMT, and HCC metastasis and by HADHA-mediated lipid programming, which may provide a new insight of miR-612 on tumor metastasis and progression. [ABSTRACT FROM AUTHOR]

• A new method of UV activated persulfate (UV/PS) to remove ARB and ARGs was proposed. • UV/PS was superior in removing ARB and ARGs relative to traditional UV treatment. • UV/PS reduced the abundance of potential pathogen bacteria resistant to UV. • OH and SO 4 – contributed to the removal of ARB and ARGs in UV/PS system. • This study provides a promising way to remove ARGs and ARB for wastewater treatment. The emerging antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) are increasingly appreciated to be as important as microbial contaminants. This paper focused on UV-activated persulfate (UV/PS), an advanced oxidation process, in removing ARB and ARGs from secondary wastewater effluent. Results showed that the inactivation efficiency of macrolides-resistant bacteria (MRB), sulfonamides-resistant bacteria (SRB), tetracyclines-resistant bacteria (TRB) and quinolones-resistant bacteria (QRB) by UV/PS reached 96.6 %, 94.7 %, 98.0 % and 99.9 % in 10 min, respectively. UV/PS also showed significant removal efficiency on ARGs. The reduction of total ARGs reached 3.84 orders of magnitude in UV/PS which is more than that in UV by 0.56 log. Particularly, the removal of mobile genetic elements (MGE) which might favor the horizontal gene transfer of ARGs among different microbial achieved 76.09 % by UV/PS. High-throughput sequencing revealed that UV/PS changed the microbial community. The proportions of Proteobacteria and Actinobacteria that pose human health risks were 4.25 % and 1.6 % less than UV, respectively. Co-occurrence analyzes indicated that ARGs were differentially contributed by bacterial taxa. In UV/PS system, hydroxyl radical and sulfate radical contributed to the removal of bacteria and ARGs. Our study provided a new method of UV/PS to remove ARGs and ARB for wastewater treatment. [ABSTRACT FROM AUTHOR]

An amendment to this paper has been published and can be accessed via the original article. [ABSTRACT FROM AUTHOR]

Highlights: [•] Preparation and characterization of titanium peroxide powder. [•] Great selective adsorption property of titanium peroxide on cationic dyes. [•] The adsorption of methylene blue, malachite green, neutral red, methyl orange and phenol by titanium peroxide. [•] Adsorption equilibrium, kinetics and mechanism of titanium peroxide on methylene blue, malachite green and neutral red. [Copyright &y& Elsevier]

Biochar has been received increasing concerns regarding its environmental effect, which is promising in wastewater treatment. In this study, the performance of β-cyclodextrin functionalized biochar (β-BC) on the removal of antibiotic resistance genes (ARGs) in wastewater treatment under the co-stresses of heavy metals and dye is evaluated. Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%–98.84% MO removal efficiency achieved in β-BC additive group, compare to 0.16 mg/L and 87.92%–94.11% of that in control, respectively, indicating that β-BC can benefits the performance of contaminants removal. To evaluate the role of β-BC plays on ARGs in multi-contaminants stressed system, tet W, tet M, sul -1, sul -2, bla TEM , oxa -1, qnr -S, erm -B and intI -1 are identified. The relative abundance of all identified ARGs are decreased when β-BC presence compared to the corresponding groups without β-BC additive. The diversity and composition of microbial community are explored and the reduction of potential antibiotic-resistant bacteria is speculated as a driver of ARGs removal. In conclusion, our study demonstrates that β-BC possesses the ability to promote the removal of ARGs during continuous wastewater treatment under HMs-MO co-contaminant. Unlabelled Image • A novel ARGs removal method using β-cyclodextrin modified biochar (β-BC) was proposed. • Azo dye leads to a co-selection pressure on antibiotic resistant genes (ARGs). • β-BC showed excellent heavy metals and azo dye removal efficiency. • β-BC exhibited efficient ability on the removal of ARGs. • This study provides a promising way to minimize the accumulation of ARGs. [ABSTRACT FROM AUTHOR]