1. Therapeutic effect of the YH6 phage in a murine hemorrhagic pneumonia model.
- Author
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Yang, Mei, Du, Chongtao, Gong, Pengjuan, Xia, Feifei, Sun, Changjiang, Feng, Xin, Lei, Liancheng, Song, Jun, Zhang, Lei, Wang, Bin, Xiao, Feng, Yan, Xinwu, Cui, Ziyin, Li, Xinwei, Gu, Jingmin, and Han, Wenyu
- Subjects
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PSEUDOMONAS aeruginosa infections , *PNEUMONIA treatment , *MULTIDRUG resistance , *THERAPEUTIC use of bacteriophages , *MICROBIAL virulence , *HOSTS (Biology) , *LABORATORY mice - Abstract
The treatment, in farmed mink, of hemorrhagic pneumonia caused by multidrug-resistant Pseudomonas aeruginosa strains has become increasingly difficult. This study investigated the potential use of phages as a therapy against hemorrhagic pneumonia caused by P. aeruginosa in a murine hemorrhagic pneumonia model. An N4-like phage designated YH6 was isolated using P. aeruginosa strain D9. YH6 is a virulent phage with efficient and broad host lytic activity against P. aeruginosa . No bacterial virulence- or lysogenesis-related ORF is present in the YH6 genome, making it eligible for use in phage therapy. In our murine experiments, a single intranasal administration of YH6 (2 × 10 7 PFU) 2 h after D9 intranasal injections at double minimum lethal dose was sufficient to protect mice against hemorrhagic pneumonia. The bacterial load in the lungs of YH6-protected mice was less than 10 3 CFU/g within 24 h after challenge and ultimately became undetectable, whereas the amount of bacteria in the lung tissue derived from unprotected mice was more than 10 8 CFU/g within 24 h after challenge. In view of its protective efficacy in this murine hemorrhagic pneumonia model, YH6 may serve as an alternative treatment strategy for infections caused by multidrug-resistant P. aeruginosa . [ABSTRACT FROM AUTHOR]
- Published
- 2015
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