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High sodium intake is associated with increased blood pressure and and increased risk of cardiovascular disease (CVD). Among the countries of the European Union, Bulgaria ranks first in mortality from CVD. Genetically determined salt sensitivity is present in 50% of the hypertensive and 26% of the normotensive population, posing a major public health problem. The present study was conducted within the National Program for Prevention of Chronic NonCommunicable Diseases 2014 - 2020 to assess the sodium intake of the population in Bulgaria. 36 normotensive participants are included in the study. DNA from each volunteers was isolated and genotyped for rs10177833 (A>C) in SLC4A5 gene by TaqMan SNP genotyping assay. To determine the 24-hour urinary excretion of sodium in the subjects, a random sample was taken from each participant. Sodium concentration was determined by atomic absorption spectrometry. A WHO protocol has been used to recalculate sodium excretion (Protocol for Sodium Determination in 24hour Urine Samples). Eighteen of total of 35 participants had wild AA genotype (51.43%), thirteen were heterozygotes (37.14%) and four were carriers of two polymorphic C alleles (11.43%). The frequency of A allele of rs10177833 (A>C) in SLC4A5 gene was 70%, whereas polymorphic C allele was only 30%. The correlation analysis showed negative statistical significant relationship between rs10177833 in SLC4A5 gene and sodium concentration (in milligrams) in 24 - hours urine (r = -0.365, p=0.031). Also the holders of CC genotype had significant lower values of Na compared to these with wild AA genotype (p=0.01) and compared to heterozygotes (p=0.019). In conclusion, we found a clear relationship between the polymorphic variant rs10177833 in SLC4A5 gene and the concentration of sodium in urine in Bulgarian normotensives. The tendency of association was found between the studied polymorphic variant and blood pressure values, which should be studied in a large number of participants. [ABSTRACT FROM AUTHOR]