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To find out whether matrix metalloproteinases, which comprise all classes of collagenases (including gelatinases), can be involved in the implementation of the antidiuretic effect of vasopressin, the effect of this hormone on the gelatinase activity of papillary renal cells in Wistar rats was studied by zymography. It was shown that the addition of the hormone to the suspension of papillary renal cells caused an increase in the gelatinase activity of proteins with a molecular weight of 52, 57 and 89 kDa. This effect was imitated by desmopressin, a vasopressin V2 receptor agonist. Vasopressin, but not desmopressin, caused an increase in the secretion of 57 and 89 kDa proteins in papillary renal cells into the incubation medium. The role of matrix metalloproteinases in vasopressin-induced increasing the water permeability of the intercellular matrix in the rat renal papilla is discussed. [ABSTRACT FROM AUTHOR]