1. Distinctive CD8+ T cell and MHC class I signatures in polycythemia vera patients.
- Author
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Cardoso, Elsa M., Esgalhado, André J., Patrão, Luís, Santos, Mónica, Neves, Vasco Pinto, Martinez, Jorge, Patto, Maria Assunção Vaz, Silva, Helena, and Arosa, Fernando A.
- Subjects
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POLYCYTHEMIA vera , *MYELOPROLIFERATIVE neoplasms , *ERYTHROCYTES , *T cells , *CELL populations , *MONOCYTES , *PATIENTS , *BONE marrow diseases , *HLA-B27 antigen , *CASE-control method - Abstract
Polycythemia vera (PV) is a myeloproliferative neoplasm characterized by overproduction of red blood cells. We have performed a comprehensive characterization of blood immune cells for expression of naïve and memory receptors as well as β2m-associated and β2m-free MHC class I heavy chains, also known as closed and open conformers, respectively, in PV patients and age-matched controls (CTR). We show that the peripheral CD3+CD8+ T cell pool in PV patients is clearly divided into two discrete populations, a more granular CD3+CD8high T cell population enriched in effector-memory CD45RA+ T cells (CD8+ TEMRA) when compared to CTR (P < 0.001), and a less granular CD3+CD8int T cell population that is completely absent in the CTR group (78 vs. 0%, P < 0.001) and is a mixture of naïve (CD8+ TN) and CD8+ TEMRA cells expressing intermediate levels of CD28, i.e., CD3+CD8intCD28int. While the percentage of CD3+CD8int TN cells correlated positively with the number of erythrocytes, the percentage of CD3+CD8int TEMRA correlated negatively with the number of platelets. Finally, we report that PV patients' lymphocytes and monocytes display lower levels of closed (W6/32+) MHC-I conformers at the cell surface while exhibiting increased amounts of open (HC-10+) MHC-I conformers. The implications of this distinctive immune signature are discussed. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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