1. Fetuin B links vitamin D deficiency and pediatric obesity: Direct negative regulation by vitamin D.
- Author
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Walker, Gillian E., Follenzi, Antonia, Bruscaggin, Valentina, Manfredi, Marcello, Bellone, Simonetta, Marengo, Emilio, Maiuri, Luigi, Prodam, Flavia, and Bona, Gianni
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VITAMIN D deficiency , *CHILDHOOD obesity , *ALPHA fetoproteins , *PROTEOMICS , *ADIPONECTIN , *BLOOD proteins , *METABOLIC disorders - Abstract
Vitamin D (VD) deficiency (VDD) correlates to obesity, with VD a recognized mediator of metabolic diseases. From a previous proteomic study identifying adiponectin as a link between VDD and pediatric obesity, herein we analysed another protein (SSP2301) increased with VDD. A focused 2D-electrophoretic analysis identified 4 corresponding plasma proteins, with one predicted to be fetuin B (FETUB). FETUB was studied due to its emerging role in metabolic diseases and cytogenetic location (3q27.3) with adiponectin. Results were confirmed in obese children, where plasma FETUB was higher with VDD. A direct effect by 1α,25-(OH)2D3 on hepatocellular FETUB synthesis was observed, with a time and dose dependent reduction. Further, we demonstrated the VD-receptor (VDR) is key, with FETUB “released” with VDR silencing. Finally, VD supplementation (6weeks) to juvenile mice fed a standard diet, reduced plasma FETUB. Only at 22weeks did liver FETUB correspond to plasma FETUB, highlighting the contribution of other VD-responsive tissues. Overall, FETUB is a key protein linking VDD to pediatric obesity. With an emerging role in metabolic diseases, we demonstrate that VD/VDR directly regulate FETUB. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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