1. The amino-terminal residue of glycoprotein B is critical for neutralization of bovine herpesvirus 1
- Author
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Okazaki, Katsunori, Fujii, Sanae, Takada, Ayato, and Kida, Hiroshi
- Subjects
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GLYCOPROTEINS , *HERPESVIRUSES , *MONOCLONAL antibodies , *IMMUNOCYTOCHEMISTRY , *IMMUNOGLOBULINS - Abstract
Abstract: In order to address the neutralization epitope on bovine herpesvirus 1 (BHV1) glycoprotein B (gB), a panel of monoclonal antibodies (MAbs), a series of truncation forms of the glycoprotein and an MAb-escape mutant were used in this study. Immunocytochemistry on the truncations using MAbs against the glycoprotein revealed that the neutralization epitopes recognized by the MAbs lay between residues 1 and 52 of mature gB. Comparison of the sequences among the mutant, parent, and revertant viruses demonstrated that the amino-terminal residue of mature gB of the escape mutant was changed from Arg to Gln. These findings indicate that the amino-terminal residue of gB is critical for neutralization of BHV1. [Copyright &y& Elsevier]
- Published
- 2006
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