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Port automation has been in the forefront of maritime innovation in the last decade. On that front, Automated Straddle Carriers (ASCs) are increasingly used to move containers efficiently. However, the introduction of ASCs in port operations can be disruptive if not handled properly, especially since the field can face many uncertainties such as increased container trade. The purpose of the paper is to investigate whether the introduction of Automated Straddle Carriers in port operations can improve the overall efficiency. To achieve the objective, a System Dynamics model was developed and tested under different scenarios. The results indicate that the introduction of ASCs is accompanied by an increase in productivity of the vehicles which results in more TEUs serviced. One of the most interesting results of the various scenarios is that for all rates of incoming TEUs, berth productivity is superior when operations are performed with 5 ASCs than with 10 manned vehicles. Finally, another issue that port authorities should always have in mind is the need for coordination among the various sub-processes and optimization of the necessary vehicles in order to avoid under-utilization of resources. [ABSTRACT FROM AUTHOR]

Abstract This paper applies a mathematical model for supporting the decision and policy making in the city of Barcelona, presenting the key performance indicators of the Barcelona taxi market, which are waiting/access time and benefits of the drivers. The model accounts for demand elasticity, which allows for estimating the optimum fare level and estimating the related demand for taxi services, which allows the taxi decision makers to have a rough estimation of the taxi sector turn over. In addition, the model is applied to a full-day time period, allowing for useful insights on the duration and distribution of the taxi shifts that should be defined by the taxi policy makers as well as the optimal operation mode for each interval of the day. Finally, the model implementation in the city of Barcelona determines that the optimum fares for the taxi sector is 0.68 euros/kilometer and the optimum number of taxis is 19 taxis/km2. In this situation, the most effective taxi policy is to have 4 days with an 8-h shift and one day with two non-consecutive 4-h shifts every week. [ABSTRACT FROM AUTHOR]

This paper presents a framework for data collection, filtering, and fusion, together with a set of operational tools to validate, analyze, utilize, and highlight the added value of probe data. Data is collected by both conventional (loops, radars, and cameras) and innovative (Floating Car Data, detectors of Bluetooth devices) technologies and refers to travel times and traffic flows on road networks. The city of Thessaloniki, Greece, serves as a case study for the implementation of the proposed framework. The methodology includes the estimation of traffic flow based on measured travel time along predefined routes and short-term forecasting of traffic volumes and their spatial expansion in the road network. The proposed processes and the framework itself have the potential of being implemented in urban road networks. [ABSTRACT FROM AUTHOR]

This study investigates the use of geo-referenced social media data for enhancing the transportation planning capabilities. A methodological framework is defined for the identification of the citizens' mobility patterns using social media data, which consists of a number of check-in events that are made through the Facebook service and 1200 taxis' floating car data for the city of Thessaloniki, during the period of February of 2016. Focus is paid on the derivation of taxi demand forecasting models using the number of check-in events through spatial statistic and analytical tools. The results illustrate the actual correlation of demand for taxis to the check-in events, while it is noted that models should take into account the temporal and spatial offset of the demand production to the check-in events. The results show that the correlation is better during the evening and night hours, since during the morning and afternoon most tips are homework based and people does not check-in at their place of work or at home. [ABSTRACT FROM AUTHOR]

In recent years, increasing attention has been drawn to the development of various applications of intelligent transportation systems (ITS), which are credited with the amelioration of traffic conditions in urban and regional environments. Advanced traveler information systems (ATIS) constitute an important element of ITS by providing potential travelers with information on the network's current performance both en-route and pre-trip. In order to tackle the complexity of such systems, derived from the difficulty of providing real-time estimations of current as well as forecasts of future traffic conditions, a series of models and algorithms have been initiated. This paper proposes the development of an integrated framework for real-time ATIS and presents its application on a large-scale network, that of Thessaloniki, Greece, concluding with a discussion on development and implementation challenges as well as on the advantages and limitations of such an effort. [ABSTRACT FROM PUBLISHER]

Data collection for the provision of real time traveller information services is a key issue, both for the travellers as well as for traffic managers. This paper presents a methodology for estimating travel times in dense urban road networks using point-to-point detectors. The aim is to fill in the gap of existing travel time estimation methodologies, which are based on point-to-point detection devices. Bluetooth (BT) is considered as one of the less expensive technologies for estimating travel times. Data filtering and data correction require rigorous methodologies, which if not correctly applied may result in inaccurate results as compared to other methods. The main difficulty of data processing is to identify the correct set of Media Access Control (MAC) addresses for estimating travel times, especially in dense urban road networks, where three main error sources exist: the co-existence of various transport modes (private vehicles, buses, pedestrians, bicycles etc.), the existence of more than one possible paths between two BT detectors and the existence of stops or trips ending between two BT detectors. These error sources create outliers that need to be identified and taken into account. The results of the proposed methodology confirm that outliers are eliminated, as shown by a case study with 10 BT detectors installed at major intersections of Thessaloniki's Central Business District (CBD). [ABSTRACT FROM PUBLISHER]

Within the framework of this paper, the existence of a Macroscopic Fundamental Diagram (MFD) for the city of Thessaloniki is discussed, obtained through simulation. Initial findings show that there is a well-defined MFD, which is a property of a network, as it is not influenced by changes in travel demand. The MFD can be used as tool for managing traffic at real-time and for improving accessibility in an area, through pricing strategies and entrance points control based on the accumulation of vehicles. [ABSTRACT FROM AUTHOR]

Map matching is a crucial data processing task for transferring measurements from the dynamic sensor location to the relevant road segment. It is especially important when estimating road network speed by using probe vehicles (floating car data) as speed measurement sensors. Most common approaches rely on finding the closet road segment, but road network geometry (e.g., dense areas, two-way streets, and superposition of road segments due to different heights) and inaccuracy in the GNSS location (up to decades of meters in urban areas) can wrongly allocate up to 30% of the measurements. More advanced methods rely on taking the topology of the network into account, significantly improving the accuracy at a higher computational cost, especially when the accuracy of the GNSS location is low. In order to both improve the accuracy of the "closet road segment" methods and reduce the processing time of the topology-based methods, the data can be pre-processed using AI techniques to reduce noise created by the inaccuracy of the GNSS location and improve the overall accuracy of the map-matching task. This paper applies AI to correct GNSS locations and improve the map-matching results, achieving a matching accuracy of 76%. The proposed methodology is demonstrated to the floating car data generated by a fleet of 1200 taxi vehicles in Thessaloniki used to estimate road network speed in real time for information services and for supporting traffic management in the city. [ABSTRACT FROM AUTHOR]

This study aimed to assess acute and residual changes in sprint-related hamstring injury (HSI) risk factors after a football (soccer) match, focusing on recovery within the commonly observed 72-h timeframe between elite football matches. We used a multifactorial approach within a football context, incorporating optical and ultrastructural microscopic analysis of BFlh (biceps femoris long head) muscle fibres, along with an examination of BFlh fibre composition. Changes in sprint performance-related factors and HSI modifiable risk factors were examined until 3 days after the match (MD +3) in 20 football players. BFlh biopsy specimens were obtained before and at MD +3 in 10 players. The findings indicated that at MD +3, sprint-related performance and HSI risk factors had not fully recovered, with notable increases in localized BFlh fibre disruptions. Interestingly, match load (both external and internal) did not correlate with changes in sprint performance or HSI risk factors nor with BFlh fibre disruption. Furthermore, our study revealed a balanced distribution of ATPase-based fibre types in BFlh, with type-II fibres associated with sprint performance. Overall, the results suggest that a 72-h recovery period may not be adequate for hamstring muscles in terms of both HSI risk factors and BFlh fibre structure following a football match. [ABSTRACT FROM AUTHOR]

• We have been able to collect demographic, genetic and clinical data of a large series of patients with Pompe disease in Spain. • The estimated prevalence of Pompe disease in Spain is lower than expected and could around 3.1 cases per every millions of habitants. • The region with a higher prevalence of cases is Andalucia, located in the South of Spain. • Our data shows a huge variability in how the diagnosis is obtained and the follow-up is performed across different medical centers in Spain, suggesting that new guidelines are required. Pompe disease is a rare genetic disorder with an estimated prevalence of 1:60.000. The two main phenotypes are Infantile Onset Pompe Disease (IOPD) and Late Onset Pompe Disease (LOPD). There is no published data from Spain regarding the existing number of cases, regional distribution, clinical features or, access and response to the treatment. We created a registry to collect all these data from patients with Pompe in Spain. Here, we report the data of the 122 patients registered including nine IOPD and 113 LOPD patients. There was a high variability in how the diagnosis was obtained and how the follow-up was performed among different centres. Seven IOPD patients were still alive being all treated with enzymatic replacement therapy (ERT) at last visit. Ninety four of the 113 LOPD patients had muscle weakness of which 81 were receiving ERT. We observed a progressive decline in the results of muscle function tests during follow-up. Overall, the Spanish Pompe Registry is a valuable resource for understanding the demographics, patient's journey and clinical characteristics of patients in Spain. Our data supports the development of agreed guidelines to ensure that the care provided to the patients is standardized across the country. [ABSTRACT FROM AUTHOR]

Transport systems are undergoing a change of paradigm that focuses on resource-sharing and collaboration of multiple and diverse stakeholders. This paper aims to present a state-of-the-art on the main research issues of multistakeholder collaboration in urban transport and address the main contributions of the Special Issue on Collaboration and Urban Transport to the field. To that end, it seems necessary to identify and address the complexity of the relations of the stakeholders in the field, beyond the traditional classification of private and public stakeholders. A functional classification of urban stakeholders related to the different land uses is proposed a refer to space users and space organizers, each with several sub-categories. Furthermore, the collaboration among those stakeholders can take different forms and can be developed at different levels: transactional, informational and decisional. Thus, the main research topics regarding multi-stakeholders' collaboration are defined as: partnerships, resource sharing, resource pooling and Mobility-as-a-Service (MaaS) systems. A set of papers in this special issue focus on Urban Consolidation Centres (UCCs), partnerships in transport under a general perspective, multi-stakeholder cooperation and its barriers, collaborative decision-making, traffic prediction and urban congestion. In the papers, which deal with the field of multi-stakeholder collaboration in urban transport, there is a predominance on the use of surveys, but also a focus on data-driven techniques. As a result, this special issue contributes not only to the theoretical aspects, but adds value to technical and methodological issues. [ABSTRACT FROM AUTHOR]

The sensor location problem is of particular importance when planning the allocation of limited field equipment intended to be used for advanced traffic management systems and traveller information services. The locations within a network that satisfy specific goals need to be carefully selected, based on predefined goals related to the effective collection of data and the subsequent estimation of traffic related information. The detection of traffic volumes is mainly associated with two purposes, the travel time and the Origin–Destination (O–D) trip matrix estimation. In this context, this paper presents a quadratic programing model, able to determine the optimal location of tracking sensors. The model is implemented in the urban road network of the city of Thessaloniki (Greece) in which specific number of sensors is installed and utilized for real-time travel time information provision. The proposed methodology models the sensor location problem under the general framework of a set covering problem, which is one of the most popular optimization problems and has been applied in many industrial problems. The results of the case study in Thessaloniki reveal that the proposed model defines the optimal location of the limited number of sensors in such a way that the network, which is created having all sensors as origin or destination of all possible paths, represents to great extent (87% of the traffic flow along the major paths) the traffic volumes of the whole road network of the city. [ABSTRACT FROM AUTHOR]

This paper presents a risk assessment algorithm that can be integrated within advanced safety monitoring systems of urban road networks. The research conducted is part of the MISS (Monitor Integrated Safety System) research project funded under the 6th Framework Program of the European Union. MISS aims to develop an innovative platform to dynamically sense and predict natural and infrastructure conditions, so that safety and efficiency of transport operations in multi-environmental scenarios are improved. The platform enables intelligent exchange of structured information between operational fleet vehicles and a Unified Operative Centre where information is elaborated and actions are planned. As part of the MISS program, efficient off- and online risk assessment methods have been designed to enable public authorities to better monitor and respond to hazardous incidents. These have been based on the analysis of stated preference questionnaires that have been filled in by expert bus drivers of the Bologna public transport agency. [ABSTRACT FROM AUTHOR]

The present paper presents the transportation planning methodology applied in the city of Nicosia, Cyprus, within the framework of Nicosia's "Integrated Mobility Master Plan". Unique characteristics of the capital of Cyprus, such as the sparse coverage, low service quality and even lower market penetration of the public transport network, the inadequate parking policy applied and the car-centred mobility culture of the local citizens have provided the motivation for a "redesign" of the classic four-step transport modelling process by combining static and dynamic, macroscopic and microscopic transportation analysis models, in order to support the city's decision makers. [ABSTRACT FROM AUTHOR]

Pathogenic variants in the mitochondrial tyrosyl-tRNA synthetase gene (YARS2) were associated with myopathy, lactic acidosis, and sideroblastic anemia (MLASA). However, patients can present mitochondrial myopathy, with exercise intolerance and muscle weakness, leading from mild to lethal phenotypes. Genes implicated in mtDNA replication were studied by Next Generation Sequencing (NGS) and whole exome sequence with the TruSeq Rapid Exome kit (Illumina, San Diego, CA, USA). Mitochondrial protein translation was studied following the Sasarman and Shoubridge protocol and oxygen consumption rates with Agilent Seahorse XF24 Analyzer Mitostress Test, (Agilent, Santa Clara, CA, USA). We report two siblings with two novel compound heterozygous pathogenic variants in YARS2 gene: a single nucleotide deletion in exon 1, c.314delG (p.(Gly105Alafs*4)), which creates a premature stop codon in the amino acid 109, and a single nucleotide change in exon 5 c.1391T>C (p.(Ile464Thr)), that cause a missense variant in amino acid 464. We demonstrate the pathogenicity of these new variants associated with reduced YARS2 mRNA transcript, reduced mitochondrial protein translation and dysfunctional organelle function. These pathogenic variants are responsible for late onset MLASA, herein accompanied by pancreatic insufficiency, observed in both brothers, clinically considered as Pearson's syndrome. Molecular study of YARS2 gene should be considered in patients presenting Pearson's syndrome characteristics and MLASA related phenotypes. [ABSTRACT FROM AUTHOR]

Objective: Anoctamin 5 (ANO5) is a putative intracellular calcium-activated chloride channel. Recessive mutations in ANO5 may present from asymptomatic hyperCKemia and exercise-induced myalgia to proximal and/or distal muscle weakness. Here we describe the clinical, pathological, and molecular findings of three unrelated patients with ANO5-related muscular dystrophy. Methods: In this retrospective study, we analyzed our database which includes 1700 muscle biopsies performed for diagnostic purposes from October 2004 to February 2019. Patients were attended by two myology experts, who performed and analyzed the muscle biopsies. Muscle biopsies were frozen in cooled isopenthane, cryostat sectioned, and routinely stained and reacted (minimum 16 stainings). A custom panel, including 115 genes (Nextera Rapid Capture, Illumina) and whole-exome sequencing analysis, was used for next-generation sequencing in cases without a definite pathological diagnosis. Results: Three patients were diagnosed with ANO5-related muscular dystrophy, with all presenting the common exon 5 mutation c.191dup plus a compound heterozygous missense mutation. They showed three different phenotypes (distal myopathy, LGMD2L, and asymptomatic hyperCKemia). Curiously, all three muscle biopsies showed different patterns, but numerous ragged-red fibers with little endomysial inflammation and partial invasion cell by T lymphocytes were observed in one. Conclusion: ANO5-related muscular dystrophy is a heterogeneous disease with different clinical phenotypes as well as different histological patterns, which may even mimic a mitochondrial myopathy. The results of this study provide further knowledge of the clinical, histological, and pathological features related to ANO5 mutations. [ABSTRACT FROM AUTHOR]

Mobility patterns and travel behavior aspects of students traveling to universities, are gaining attention, as the completion of such every day trips are rather complicated. University students form a social group, essentially autonomous in terms of mode choice decision making. Understanding the mode choice decision process, may reveal the most appropriate interventions for achieving sustainable transport goals. The current study aims to examine various aspects of university students' travel bahaviour such as travel distance, travel time, comfort and safety in the city of Xanthi, Greece. Moving a step forward, the paper provides a better understanding of students' travel patterns in two different environments due to the University relocation from an urban environment to a more isolated one. For this purpose, a questionnaire survey is conducted where students describe their travel habits before and after the relocation. In addition, students are given a choice set comprised of eight different factors hypothesized to internalize the effect of the relocation on mode choice for the trips to and from the University and they are asked to prioritize them ranking them from the most significant one to the least important. For the analysis two Multinomial Logit models are developed. The results verify initial considerations; distance and time are the most important factors for both cases while the use of public transport instead of walking increases the importance of economy and safety. [ABSTRACT FROM AUTHOR]

Sporadic inclusion body myositis (sIBM) is one of the most common myopathies in elderly people. Mitochondrial abnormalities at the histological level are present in these patients. We hypothesize that mitochondrial dysfunction may play a role in disease aetiology. We took the following measurements of muscle and peripheral blood mononuclear cells (PBMCs) from 30 sIBM patients and 38 age- and gender-paired controls: mitochondrial DNA (mtDNA) deletions, amount of mtDNA and mtRNA, mitochondrial protein synthesis, mitochondrial respiratory chain (MRC) complex I and IV enzymatic activity, mitochondrial mass, oxidative stress and mitochondrial dynamics (mitofusin 2 and optic atrophy 1 levels). Depletion of mtDNA was present in muscle from sIBM patients and PBMCs showed deregulated expression of mitochondrial proteins in oxidative phosphorylation. MRC complex IV/citrate synthase activity was significantly decreased in both tissues and mitochondrial dynamics were affected in muscle. Depletion of mtDNA was significantly more severe in patients with mtDNA deletions, which also presented deregulation of mitochondrial fusion proteins. Imbalance in mitochondrial dynamics in muscle was associated with increased mitochondrial genetic disturbances (both depletion and deletions), demonstrating that proper mitochondrial turnover is essential for mitochondrial homoeostasis and muscle function in these patients. [ABSTRACT FROM AUTHOR]

Sporadic inclusion body myositis (sIBM) is a rare disease that is difficult to diagnose. Muscle biopsy provides three prominent pathological findings: inflammation, mitochondrial abnormalities and fibber degeneration, represented by the accumulation of protein depots constituted by β-amyloid peptide, among others. We aim to perform a screening in plasma of circulating molecules related to the putative etiopathogenesis of sIBM to determine potential surrogate biomarkers for diagnosis. Plasma from 21 sIBM patients and 20 age- and gender-paired healthy controls were collected and stored at -80°C. An additional population of patients with non-sIBM inflammatory myopathies was also included (nine patients with dermatomyositis and five with polymyositis). Circulating levels of inflammatory cytokines (interleukin [IL]-6 and tumor necrosis factor [TNF]-α), mitochondrial-related molecules (free plasmatic mitochondrial DNA [mtDNA], fibroblast growth factor-21 [FGF-21] and coenzyme-Q10 [CoQ]) and amyloidogenic-related molecules (beta-secretase-1 [BACE-1], presenilin-1 [PS-1], and soluble Aβ precursor protein [sAPPβ]) were assessed with magnetic bead-based assays, real-time polymerase chain reaction, enzyme-linked immunosorbent assay (ELISA) and high-pressure liquid chromatography (HPLC). Despite remarkable trends toward altered plasmatic expression of inflammatory and mitochondrial molecules (increased IL-6, TNF-α, circulating mtDNA and FGF-21 levels and decreased content in CoQ), only amyloidogenic degenerative markers including BACE-1, PS-1 and sAPPβ levels were significantly increased in plasma from sIBM patients compared with controls and other patients with non-sIBM inflammatory myopathies (p < 0.05). Inflammatory, mitochondrial and amyloidogenic degeneration markers are altered in plasma of sIBM patients confirming their etiopathological implication in the disease. Sensitivity and specificity analysis show that BACE-1, PS-1 and sAPPβ represent a good predictive noninvasive tool for the diagnosis of sIBM, especially in distinguishing this disease from polymyositis. [ABSTRACT FROM AUTHOR]

Studies vascular sequestration of parasitized erythrocytes in Plasmodium falciparum malaria. Pathology and immunohistochemistry of the muscuvasculature of skeletal muscle biopsy samples from malaria patients; Sequestration of parasitized red blood cells in skeletal muscle vessels; Consistency of vascular cell adhesion molecule-1 and E-selectin expression.

A woman with scleroderma and classic polyarteritis nodosa (PAN) who developed idiopathic myelofibrosis (IM) is reported. The patient presented with a one-year history of weakness, polyarthritis, Raynaud phenomenon, dry cough, and epigastralgia. The diagnosis of scleroderma with visceral involvement was made and treatment with prednisone subsequently started, with good clinical response. Six years later, fever, weight loss, livedo reticularis, and dysesthesias developed. Electromyographic studies were consistent with sensory neuropathy and a sural nerve biopsy yielded the diagnosis of PAN. The patient received cyclophosphamide plus prednisone with a favorable response, but 11 years later she was admitted because of weakness, constitutional symptoms, and abdominal pain due to spleen infarcts. Marked anemia, with aniso-poikilocytosis, tear-drop cells, immature myeloid precursors in the peripheral blood, and an increased serum LDH, was observed and the diagnosis of IM established by bone marrow biopsy. This case represents a new association between IM and an autoimmune disease and supports the hypothesis of an immune basis of IM in some patients. [ABSTRACT FROM AUTHOR]