1. HLH-30/TFEB mediates sexual dimorphism in immunity in <italic>Caenorhabditis elegans</italic>.
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Sohn, Jooyeon, Kwon, Sujeong, Lee, Gee-Yoon, Kim, Sieun S., Lee, Yujin, Lee, Jongsun, Jung, Yoonji, Ham, Seokjin, Park, Hae-Eun H., Park, Sangsoon, Ha, Seokjun G., Lee, Daehan, and Lee, Seung-Jae V.
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SEXUAL dimorphism , *TRANSCRIPTION factors , *CAENORHABDITIS elegans , *IMMUNITY , *PATHOGENIC bacteria , *IMMUNE response - Abstract
Sexual dimorphism affects various biological functions, including immune responses. However, the mechanisms by which sex alters immunity remain largely unknown. Using
Caenorhabditis elegans as a model species, we showed that males exhibit enhanced immunity against various pathogenic bacteria through the upregulation of HLH-30 (Helix Loop Helix 30/TFEB (transcription factor EB)), a transcription factor crucial for macroautophagy/autophagy. Compared with hermaphroditicC. elegans , males displayed increased activity of HLH-30/TFEB, which contributed to enhanced antibacterial immunity.atg-2 (AuTophaGy (yeast Atg homolog) 2) upregulated by HLH-30/TFEB mediated increased immunity in maleC. elegans . Thus, the males appear to be equipped with enhanced HLH-30/TFEB-mediated autophagy, which increases pathogen resistance, and this may functionally prolong mate-searching ability with reduced risk of infection.Abbreviations: atg-2 : AuTophaGy (yeast Atg homolog) 2; FUDR: 5-fluoro-2’-deoxyuridine; GSEA: gene set enrichment analysis; HLH-30: Helix Loop Helix 30;LC3 : microtubule associated protein 1 light chain 3; NGM: nematode growth media; RNA-seq: RNA sequencing; SEM: standard error of the mean; TFEB: transcription factor EB; WT: wild-type [ABSTRACT FROM AUTHOR]- Published
- 2024
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