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An three-dimensional (3D) porous structure graphene oxide nanoribbons (GONRs) aerogel has been prepared via hydrothermal method to overcome the challenges of solid–liquid separation for powdered carbon-based nanomaterials. GONRs aerogel showed low density, good mechanical strength and easy separation from water. Uranium(VI) and thorium(IV) adsorption by GONRs aerogel was investigated by batch experiments, demonstrating their strongly pH-dependent, spontaneous and endothermic adsorption processes. GONRs aerogel exhibited the maximum U(VI)- and Th(IV)-uptake capacity (430.6 and 380.4 mg g−1, respectively) due to its large specific area (597.4 m2 g−1) and abundant oxygen-containing groups. This work suggests that GONRs aerogel has great potential for treatment of uranium and thorium-containing effluents. [ABSTRACT FROM AUTHOR]

A facile approach for graphene modification based on 1 MeV electron irradiation is proposed here to produce a graphene/Si betavoltaic cell. Compared to the unirradiated graphene, graphene-modified under the dose rate of 5 kGy/pass (with accumulated doses of 10 kGy) and 10 kGy/pass (with accumulated doses of 20 kGy) electron irradiation, the output power density of graphene/Si betavoltaic cells were increased by 2 and 3 times, respectively. The effect of different dose rates (5–20 kGy/pass) and accumulated irradiation doses (0–40 kGy) electron irradiation on the doping, elemental morphology, and the magnitude of induced defects of graphene were further studied. The material characterization techniques demonstrated that different dose rates and accumulated doses of electron irradiation can introduce varying degrees of partial defects in graphene, resulting in different regulations of doping and control band gaps of graphene. Concurrently, the results of XPS and Raman spectroscopy revealed that the sp2 hybrids of graphene can be converted into sp3 hybrids by appropriate electron irradiation and has light N-type doping characteristics. Thus, the irradiated graphene/Si betavoltaic cells can maintain higher open circuit voltage. Therefore, the graphene-modified by appropriate electron irradiation provides a facile strategy to improve the output performance of graphene/Si betavoltaic cells. [ABSTRACT FROM AUTHOR]

• A two-branch structure and a learnable upsampling strategy DUpsampling is adopted to construct an accurate ice semantic segmentation network. • A new Downsampling module and a dense connection block in the context branch is designed to construct a real-time ice semantic segmentation network. • Two river ice segmentation datasets NWPU_YRCC_EX and NWPU_YRCC2 are enlarged and relabeled. • The proposed method can obtain better results in practical application. River ice semantic segmentation is a crucial task, which can provide us with information for river monitoring, disaster forecasting, and transportation management. Previous works mainly focus on higher accuracy acquirement, while efficiency is also important for reality usage. In this paper, a real-time and accurate river ice semantic segmentation network is proposed, named FastICENet. The general architecture consists of two branches, i.e., a shallow high-resolution spatial branch and a deep context semantic branch, which are carefully designed for the scale diversity and irregular shape of river ice in remote sensing images. Then, a novel Downsampling module and a dense connection block based on a lightweight Ghost module are adopted in the context branch to reduce the computation cost. Furthermore, a learnable upsampling strategy DUpsampling is utilized to replace the commonly used bilinear interpolation to improve the segmentation accuracy. We deploy detailed experiments on three publicly available datasets, named NWPU_YRCC_EX, NWPU_YRCC2, and Alberta River Ice Segmentation Dataset. The experimental results demonstrate that our method achieves state-of-the-art performance with competing methods, on the NWPU_YRCC_EX dataset, we can achieve the segmentation speed as 90.84FPS and the segmentation accuracy as 90.770 % mIoU, which also illustrates the good leverage between accuracy and speed. Our code is available at https://github.com/nwpulab113/FastICENet [ABSTRACT FROM AUTHOR]

Glycoprotein D (gD) of bovine herpesvirus-1 (BoHV-1) is essential for attachment and penetration of cells during infection and is a major target for neutralizing antibodies during an adaptive immune response. Currently there are no recombinant antibodies capable of binding gD epitopes for use in treating BoHV-1 infection. In this study, a bovine scFv gene derived from a hybridoma secreting monoclonal antibodies (McAbs) against the amino acid motif MEESKGYEPP of gD was expressed in E. coli. Molecular modeling, western blot and ELISA analysis showed that this scFv had a high affinity for BoHV-1 gD, with a Kd of 161.2 ± 37.58 nM and for whole BoHV-1 virus, with a Kd of 67.44 ± 16.99 nM. In addition, this scFv displayed a high affinity for BoHV-1 antigen in an ELISA and competed with BoHV-1 anti-serum in a competitive ELISA. Immunofluorescence assay (IFA) and laser confocal microscopy showed that this scFv could efficiently bind to and be internalized by BoHV-1 infected Madin-Darby bovine kidney (MDBK) cells. Importantly, this scFv was shown to inhibit BoHV-1 infectivity and to reduce the number of viral plaques by blocking viral attachment to MDBK cells. Our study suggests that this bovine single-chain antibody could be developed for use as a diagnostic and therapeutic agent against BoHV-1 infection in cattle. [ABSTRACT FROM AUTHOR]

Porcine epidemic diarrhea (PED), caused by porcine epidemic diarrhea virus (PEDV), is a highly contagious, acute enteric viral disease of swine characterized by vomiting, watery diarrhea, dehydration and death. To identify and characterize the field PEDVs associated with the outbreaks of severe diarrhea in piglets in Jiangxi, 2013, the complete genome sequences of two representative strains of PEDV, designated CH/JX-1/2013 and CH/JX-2/2013, were determined and analyzed. The genome sequences of both emergent Jiangxi PEDV strains, CH/JX-1/2013 and CH/JX-2/2013, were 28,038 nucleotides in length excluding 3’ poly (A) tail. Compared to the PEDV CV777 strain, CH/JX-1/2013 and CH/JX-2/2013 had some unique genetic characteristics in the proximal region of the 5´-UTRs. Phylogenetic analysis of the complete genomes and the structural proteins revealed that CH/JX-1/2013 and CH/JX-2/2013 had a close relationship with post-2010 Chinese PEDV strains and US strains identified in 2013. The nucleotide identity between the two Jiangxi strains (CH/JX-1/2013 and CH/JX-2/2013) and 30 strains of PEDV identified ante-2010 and post-2010 ranged from 96.3–97.0% and 97.3–99.7%, respectively. Multiple nucleotide and deduced amino acid mutations were observed in the ORF1a/b, S, ORF3, E, M and N genes among the current field PEDV strains when compared to the CV777 strain. Some of the mutations altered the amino acid charge and hydrophilicity, and notably, there was an amino acid substitution in the middle of one neutralizing epitope (L1371I) of the S gene of both CH/JX-1/2013 and CH/JX-2/2013. Taken together, the accumulated genetic variations of the current field PEDV strains might have led to antigenic changes of the viruses, which might confer the less effectiveness or failure of the CV777-based vaccines currently being widely used in Jiangxi, China. [ABSTRACT FROM AUTHOR]

Simple Summary: Porcine deltacoronavirus (PDCoV) is an emerging enteropathogen distributed globally, which causes substantial economic losses in the swine industry. The characterization of the receptor promiscuity may pose a risk of cross-species transmission. However, the options for pharmaceutical interventions are limited. In this study, the vectors expressing shRNAs targeting the nucleocapsid gene were generated to assess the inhibition effect of PDCoV reproduction. Our preliminary results demonstrate that a dual shRNA expression system is an effective strategy in combating PDCoV infection without cytotoxicity, which would facilitate the ongoing development of RNAi-based therapeutic drugs against viral diseases. Porcine deltacoronavirus (PDCoV) is a novel enteric coronavirus and is becoming one of the major causative agents of diarrhea in pig herds in recent years. To date, there are no commercial vaccines or antiviral pharmaceutical agents available to control PDCoV infection. Therefore, developing a reliable strategy against PDCoV is urgently needed. In this study, to observe the antiviral activity of RNA interference (RNAi), four short hairpin RNAs (shRNAs) specific to the nucleocapsid (N) gene of PDCoV were designed and tested in vitro. Of these, a double-shRNA-expression vector, designated as pSil-double-shRNA-N1, was the most effectively expressed, and the inhibition of PDCoV replication was then further evaluated in neonatal piglets. Our preliminary results reveal that plasmid-based double-shRNA-expression targeting the N gene of PDCoV can significantly protect LLC-PK1 cells and piglets from pathological lesions induced by PDCoV. Our study could benefit the investigation of the specific functions of viral genes related to PDCoV infection and offer a possible methodology of RNAi-based therapeutics for PDCoV infection. [ABSTRACT FROM AUTHOR]

Assembling graphene oxide nanoribbons (GONRs) into three-dimensional (3D) materials with controllable and desired structure is an effective way to expand their structural features and enable their practical applications. In this work, an ultralight 3D porous amidoxime functionalized graphene oxide nanoribbons aerogel (PAO/GONRs-A) was prepared via solvothermal polymerization method using acrylonitrile as monomer and GONRs as solid matrices for selective separation of uranium(VI) from water samples. The PAO/GONRs-A possessed a high nitrogen content (13.5%), low density (8.5 mg cm−3), and large specific surface area (494.9 m2 g−1), and presented an excellent high adsorption capacity of uranium, with a maximum capacity of 2.475 mmol g−1 at a pH of 4.5, and maximum uranium-selectivity of 65.23% at a pH of 3.0. The results of adsorption experiments showed that U(VI) adsorption on PAO/GONRs-A was a pH-dependent, spontaneous and endothermic process, which was better fitted to the pseudo-second-order kinetic model and Langmuir isotherm model. Both X-ray photoelectron spectroscopy (XPS) and density functional theory (DFT) calculations revealed that U(VI) adsorption on PAO/GONRs-A mainly did rely on the amidoxime groups anchored on the aerogel while UO 2 (PAO) 2 (H 2 O) 3 was dominant after interaction of uranyl with PAO/GONRs-A. Therefore, as a candidate adsorbent, PAO/GONRs-A has a high potential for the removal of uranium from aqueous solutions. Unlabelled Image • An easy-to-separate graphene oxide nanoribbons aerogel was synthesized. • The aerogel showed low density, large specific surface area and high nitrogen content. • The aerogel presented high adsorption capacity and selectivity for uranium. • DFT calculations revealed the coordination modes of U(VI) with the aerogel. [ABSTRACT FROM AUTHOR]

Simple Summary: Porcine circovirus 3 (PCV3) is a new species of PCV that was associated with porcine dermatitis and nephropathy syndrome (PDNS), respiratory disease, cardiac and multisystem inflammation in nursery and finishing pigs, and reproductive failure problems, including abortion, mummified fetuses, and stillbirth in sows. To date, the reports on the PCV3 present in diarrhea pigs are limited and the genetic characteristics of PCV3 from diarrheal pigs and pathogenicity on pigs were inconsistent. This study aimed to investigate the prevalence of PCV3 in pigs with/without diarrhea, to analyze the genome sequence of PCV3 from diarrheal pigs, and to inquire into the associated pathogenicity of PCV3 to piglets experimentally infected with PCV3-positive intestinal contents. The results demonstrated that PCV3 was widely circulating in diarrheal suckling and weaned piglets. Clinical signs, gross lesions, and histological changes were observed in suckling piglets inoculated with PCV3. The complete genome of a PCV3a strain was determined and two mutations (V24A and K27R) were present when compared with PCV3b strains. The findings of this study increase the knowledge of the epidemiology, viral genetics, pathogenicity, and pathogenesis of PCV3. Porcine circovirus 3 (PCV3) infections have been reported in different clinical presentations. However, the prevalence and pathogenicity of PCV3 associated with diarrhea in piglets have been limited. Herein, we present an investigation and genome analyses of PCV3 in piglets experiencing diarrhea, and observed clinical signs, gross lesions, and histological changes in pigs negative for all known pathogens associated with diarrhea but positive for PCV3 alone. Among the feces (n = 141) tested, 16.31% (23/141) were positive for PCV3. Of which, 27.28% (15/55) and 14.29% (5/35) were present in diarrheal samples from suckling and weaned piglets, respectively. Moderate to severe atrophic villi was confined in duodenum, jejunum, and ileum, and significantly decreased average heights of villi, and the depths of crypt were observed in PCV3-infected piglets. The complete genome of a representative strain of PCV3, designated as JX/CH/2018, was determined. Multialignment analysis indicated that JX/CH/2018 had 97.7–99.7% nucleotide identity at the complete genome level, and 97.2–100% at the amino acid level of the capsid protein when compared with reference PCV3 strains. Phylogenetic analysis showed that the PCV3 strain identified in this study belonged to PCV3a lineage. The present study demonstrated that PCV3 is a common virus in diarrheal suckling and weaned piglets. [ABSTRACT FROM AUTHOR]

Simple Summary: Porcine epidemic diarrhea (PED), caused by PED virus (PEDV), is a devastating enteric disease in pigs worldwide. At least two genotypes (G1 and G2) and five subgenotypes (G1a, G1b, G2a, G2b, andG2c) of PEDV strains have been identified. To date, the reports on the antigenicity and immunogenicity of those viruses are limited and the results documented on cross-neutralization among different genotypes and/or subgenotypes of PEDV were inconsistent. This study aimed to observe the comparative pathogenicity and cross-protection between G1a and G2a PEDVs, and thus find a new insight into the antigenicity and immunogenicity of PEDVs. The results of the present study demonstrated that the G2a-based inactivated vaccine could provide sterilizing immunity against both highly virulent homologous and heterologous PEDV challenges. In contrast, the G1a-based inactivated vaccine could induce a sterilizing immune response against challenge of homologous strain CV777 and only provide partial protection for the challenge of a heterologous G2a PEDV CH/JX/01. The findings of this study might explain the underlying mechanism that severe PED and deaths still occurred among the neonatal piglets of which CV777-based PEDV vaccine were administered in China, and imply G2a-based PEDV vaccine used in this study might be a good vaccine candidate for PEDV which may provide solid protection against circulating highly virulent PEDVs. To date, two genotypes, i.e., genotype 1 (G1) and genotype 2 (G2), of porcine epidemic diarrhea virus (PEDV) have been identified in swine, while the cross protection between the G2a and G1a subgenotypes is undetermined. Hence, in the present study, we attempted to observe a comparative pathogenicity and cross protection of G1a (CV777) and G2a (CH/JX/01) PEDVs. Initially pregnant sows were vaccinated twice with the two kinds of inactivated G1a- and G2a-based PEDV vaccines, respectively and the delivered neonatal piglets were challenged with prototype isolates of G1a and G2a PEDVs, and then the pathogenicity and cross-protection in neonatal piglets were observed. The results showed that CH/JX/01, a highly virulent and dominant G2a PEDV strain currently circulating in China had more severe pathogenicity in vitro and in vivo, and induced more strong immune responses, including higher titers of sIgA in maternal milk than that induced by CV777 PEDV, a prototype of G1a PEDV strain. All piglets from the sows immunized with CH/JX/01 could not only survive when challenged with the homologous PEDV, but also be fully protected when challenged with heterogenous G1a PEDV. In contrast, the piglets from the sows immunized with CV777 could be protected when challenged with homologous PEDV and only partially protected when challenged with heterologous G2a strain of PEDV (CH/JX/01). The findings of this study provide new insights into the pathogenicity, antigenicity, and immunogenicity of currently circulating wild type G2a PEDV, which might be valuable for the development of novel PEDV vaccine candidates with improved efficacy. [ABSTRACT FROM AUTHOR]