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Light and water availability are likely to vary over the lifespan of closed-canopy forest trees, with understory trees experiencing greater limitations to growth by light and canopy trees greater limitation due to drought. As drought and shade have opposing effects on isotope discrimination (Δ13C), paired measurement of ring width and Δ13C can potentially be used to differentiate between water and light limitations on tree growth. We tested this approach for Cedrela trees from three tropical forests in Bolivia and Mexico that differ in rainfall and canopy structure. Using lifetime ring width and Δ13C data for trees of up to and over 200 years old, we assessed how controls on tree growth changed from understory to the canopy. Growth and Δ13C are mostly anti-correlated in the understory, but this anti-correlation disappeared or weakened when trees reached the canopy, especially at the wettest site. This indicates that understory growth variation is controlled by photosynthetic carbon assimilation due to variation in light levels. Once trees reached the canopy, inter-annual variation in growth and Δ13C at one of the dry sites showed positive correlations, indicating that inter-annual variation in growth is driven by variation in water stress affecting stomatal conductance. Paired analysis of ring widths and carbon isotopes provides significant insight in what environmental factors control growth over a tree's life; strong light limitations for understory trees in closed-canopy moist forests switched to drought stress for (sub)canopy trees in dry forests. We show that combined isotope and ring width measurements can significantly improve our insights in tree functioning and be used to disentangle limitations due to shade from those due to drought. [ABSTRACT FROM AUTHOR]

Nearly 13,000 years ago, the warming trend into the Holocene was sharply interrupted by a reversal to near glacial conditions. Climatic causes and ecological consequences of the Younger Dryas (YD) have been extensively studied, however proxy archives from the Mediterranean basin capturing this period are scarce and do not provide annual resolution. Here, we report a hydroclimatic reconstruction from stable isotopes (δ18O, δ13C) in subfossil pines from southern France. Growing before and during the transition period into the YD (12 900–12 600 cal BP), the trees provide an annually resolved, continuous sequence of atmospheric change. Isotopic signature of tree sourcewater (δ18Osw) and estimates of relative air humidity were reconstructed as a proxy for variations in air mass origin and precipitation regime. We find a distinct increase in inter-annual variability of sourcewater isotopes (δ18Osw), with three major downturn phases of increasing magnitude beginning at 12 740 cal BP. The observed variation most likely results from an amplified intensity of North Atlantic (low δ18Osw) versus Mediterranean (high δ18Osw) precipitation. This marked pattern of climate variability is not seen in records from higher latitudes and is likely a consequence of atmospheric circulation oscillations at the margin of the southward moving polar front. [ABSTRACT FROM AUTHOR]

According to the Intergovernmental Panel on Climate Change (IPCC), all of Africa is very likely to warm up more than the global average during this century. Especially (semi-)arid regions are expected to experience particularly high warming and possibly catastrophic droughts. However, assessments of the impacts of climate change on these regions are currently impeded by a lack of transregional high temporal resolution proxy data for the African continent. Baobab trees are widely distributed in (semi-)arid Africa and can reach ages of up to 2000 years. This pilot study was aimed at investigating African baobabs, Adansonia spp., from a site in Botswana using multiple dendroclimatological methods. Increment cores from 16 individual baobabs growing on Kubu Island (20°53' S, 25°49' E), a granite pluton located in the Kalahari, were collected in June 2011 to test for annual growth and the species' utility for palaeoclimatic studies. Due to the particular wood fabric and relatively high water content, baobab increment cores were packed in air-tight opaque tubes and stored in a freezer to prevent drying and mould formation. The complicated wood anatomical structure was found to be analysed best using a microscope with incident UV light, allowing tree-ring boundaries to be distinguished. Nonetheless, potential differences in individual site conditions, as well as diverse tree ages, caused conventional dendrochronological crossdating to fail. Missing and false tree rings could be identified due to the strong relationship between tree-ring width and annual precipitation amount allowing the development of a preliminary 50 year-long baobab chronology (1960–2009). Subsequently, stable carbon and oxygen isotope analyses revealed significant correlations of Δ13C and δ18O of tree rings with climate data. Year-to-year isotope variability and trends were found to be in good agreement with established models of fractionation. Intrinsic water-use efficiency has mainly increased over the study period (2–30 %). Despite the demonstrated high potential of African baobabs as a valuable high-resolution climatearchive, we conclude that more dendrochronological calibration studiesare required at various sites in southern Africa. Furthermore, ecophysiological monitoring of climate and stable isotope signal transfer from the atmosphere, through soil and leaves into the tree rings is necessary to fully understand tree-ring formation and climate response of the African baobab. [ABSTRACT FROM AUTHOR]

Previous studies have suggested that the Late Glacial period (LG; ∼14 600–11 700 cal BP) was characterised by abrupt and extreme climate variability over the European sector of the North Atlantic. The limited number of precisely dated, high-resolution proxy records, however, restricts our understanding of climate dynamics through the LG. Here, we present the first annually-resolved tree-cellulose stable oxygen and carbon isotope chronology (δ18O tree , δ13C tree) covering the LG between ∼14 050 and 12 795 cal BP, generated from a Swiss pine trees (P. sylvestris ; 27 trees, 1255 years). Comparisons of δ18O tree with regional lake and ice core δ18O records reveal that LG climatic changes over the North Atlantic (as recorded by Greenland Stadials and Inter-Stadials) were not all experienced to the same degree in the Swiss trees. Possible explanations include: (1) LG climate oscillations may be less extreme during the summer in Switzerland, (2) tree-ring δ18O may capture local precipitation and humidity changes and/or (3) decayed cellulose and various micro-site conditions may overprint large-scale temperature trends found in other δ18O records. Despite these challenges, our study emphasises the potential to investigate hydroclimate conditions using subfossil pine stable isotopes. • Millennial stable isotope chronologies of δ13C and δ18O from 27 Swiss subfossil pine trees, covering the Late Glacial (∼14 050–12 795 cal BP). • Two extreme δ18O tree depletions parallel North Atlantic 'cool periods' (GI-1c, GS-1), another LG event (GI-1b) is not expressed in δ18O tree. • Trees with less extensive decay have significantly higher inter-isotope (δ18O tree : δ13C tree) correlations. [ABSTRACT FROM AUTHOR]

The sensitivity of European lowlands to the hydrological impacts of climate change has manifested in flood and drought events of increasing severity over recent decades. Reconstructing the long-term history of hydroclimatic fluctuations in this region is critical to our understanding of its current and future ecosystem dynamics. This is especially important considering that this region is widely susceptible to anthropogenic influences (e.g. large monoculture farms and forests, vast drained peatlands), and it also contains unique, near-natural habitats, including registered UNESCO World Heritage sites (e.g. Müritz National Park). In this study, we use annually resolved multi-centennial records of stable carbon and oxygen isotope chronologies from tree ring cellulose in lowland (50-90m asl.) pine and oak trees (P. sylvestris, Q. petraea) in NE-Germany to reconstruct hydroclimatic variability. Using a calibration and verification approach, we have evaluated the response to instrumental climate data and trends in variables such as local temperature, precipitation, drought indices (PDSI, SPEI), soil moisture, evapotranspiration potential (ETP) and vapor pressure. While ring widths correlate strongly to winter temperature in the study region, strong correlations with summer hydroclimatic conditions were found for both tree-ring 13C and 18O. The strongest relationships occur with ETP, soil moisture, and SPEI. From 1979 to 2010, current-year ETP (JJA) and soil moisture (JJA) show no significant relationships to 13C, but they are strongly correlated to 18O (r=0.66, ETP; r=-0.65, soil moisture). Slightly weaker correlations exist with SPEI (JJA) (r=-0.53, 18O; r=-0.42, 13C). In general, climate relationships with 13C are consistently weaker than those with 18O, but all correlations are significant at the 99% level. Based on a detailed assessment of stable isotope-climate relationships and resulting reconstruction of hydroclimatic baseline variability, we will attempt to assess the sensitivity of tree ring stable isotopes to summer drought. Special attention will be paid to the most recent droughts of 2003 and 2018, the latter of which is considered unprecedented in view of instrumental weather records. Wood anatomical analyses of tree rings from 2018 have revealed that most pine trees formed relatively few latewood cells this year. In contrast, oak tree rings showed normal latewood to earlywood proportions in 2018. Investigation of this discrepancy by additional intra-annual isotope analyses will inform the discussion of the possible limitations of using stable isotope records to reconstruct severe droughts. [ABSTRACT FROM AUTHOR]

Water stable isotope ratios of tropical precipitation predominantly reflect moisture source and precipitation intensity. Trees can incorporate the isotopic signals into annual tree-ring cellulose records, permitting reconstruction of the temporal changes of hydroclimate over decades to millennia. This is especially valuable in the Himalayas where the understanding of monsoon dynamics is limited by the lack of a dense and representative observational network. We have analyzed tree ring δ 18 O records from two distinct physiographic sites along the upper Kali Gandaki valley in the central Nepal Himalayas, representing the wet High-Himalayas and the Trans-Himalayan dryland to the north. Empirical correlations and regression analyses were compared to an in-situ calibrated oxygen isotope fractionation model, exploring the relationships between tree ring δ 18 O and seasonal-mean variability of hydroclimatic forcing at the different locations. For this purpose, gridded precipitation data from the Asian rain gauge dataset APHRODITE, as well as high resolution onsite observations (relative humidity, air temperature, δ 18 O of precipitation and radial tree growth) were used. We found that two distinct sets of meteorological values, reflecting pre-monsoon and monsoon conditions, are needed to reproduce the measured tree ring δ 18 O values from the High-Himalayan site, but that a single set of monsoonal values performs best for the Trans-Himalayan site. We conclude that Trans-Himalayan trees capture long-term changes in strength of the Indian summer monsoon. In contrast, High-Himalayan tree ring δ 18 O records a more complex hydro-climatic signal reflecting both pre-monsoon and monsoon seasons with very contrasting isotopic signatures of precipitation. This difference in the two hydroclimatic proxy records offers an opportunity to reconstruct first-order hydroclimate conditions, such as local precipitation rates, and to gain new insights into monsoon timing and seasonal water source determination across the Himalayan orographic region. • Hydroclimate seasonality is preserved in tree ring δ 18 O from the Central Himalayas. • A fractionation model confirms relationships from empirical correlation analyses. • High-Himalayan tree ring δ 18 O compounds pre- and monsoon signals • Trans-Himalayan tree ring δ 18 O only contains monsoon signal. • At different locations across the Himalayas, different climate features are recorded. [ABSTRACT FROM AUTHOR]

In recent decades, the Tibetan plateau (TP) experienced a distinctive temperature increase, with fundamental consequences for the hydrological system. As meteorological time-series extending back more than 60 years are scarce, there is a strong need for proxy data providing insight into the regional hydroclimatic history as well as the long-term variability. Within this study, a recently developed annually resolved 1500 year-long tree-ring stable oxygen isotope (δ 18 O) time from the southeastern TP is presented. Climate–proxy relationships reveal a strong impact of relative humidity (rH) during the summer months (May to September) on tree-ring δ 18 O, explaining around 45% of its variance. The derived reconstruction of relative humidity reveals a recent trend towards drier conditions on the TP. However, the present low rH level is not unprecedented in regard to the last 1500 years. In comparison to recent climate conditions, the Medieval Warm Period (MWP) was characterized by more stable conditions with higher humidity values. Hydroclimatic conditions during the Little Ice Age (LIA) suggest a contrasting two-phased period, with a clear shift from drier conditions prevailing between 1400 and 1650 AD to more humid conditions since the second half of the 17th century. Comparisons with other local proxies from lake levels and Pollen data from the southern part of the Tibetan plateau indicate a common regional climate forcing during the MWP and the LIA which can be related to changes in summer monsoon activity. However, the strength of the distinct dryness trend during the 20th century seems unique. [ABSTRACT FROM AUTHOR]

Stable oxygen isotopes records (δ18O) in tree-rings are commonly used to assess the response of trees to environmental variability being a valuable tool for studying past climate at different temporal and spatial scales. This is particularly relevant in semi-arid regions like the southern Tropical Andes, where ongoing environmental changes coincide with a rapidly increasing demand for hydrological resources, presenting a challenge for ecosystem dynamics and water resource management. In this study, we aim to determine the main spatio-temporal variability of a new network of δ18O Polylepis tarapacana chronologies during the last century, and their relationships with hydroclimate and tropical circulation at local to subcontinental scales throughout the Tropical Andes. For this purpose, we develop six δ18O P. tarapacana tree-ring chronologies across a 450 km latitudinal moisture gradient in the southern Tropical Andes adjacent to the Atacama Desert, covering the period 1900–2007. Results show a clear latitudinal gradient in the δ18O values across the network and significant relationships are observed with other δ18O tree-ring chronologies in Tropical South America, demonstrating clear regional climate influences at a subcontinental scale. A principal component analysis of the δ18O tree-ring chronologies demonstrate a strong regional environmental signal contained in the network, exhibiting a main temporal pattern (PC1 δ18O) that explains 63% of the total variance during the period 1900–2007. Comparisons between PC1 δ18O and environmental variables showed significant negative relationships with precipitation and soil moisture, and positive relationships with temperature and vapor pressure deficit (VPD) during summer when the South American monsoon occurs. The main δ18O tree-ring network signal clearly records tropical atmospheric and circulation patterns across South America. The easterly wind flux conditions from the Amazon basin favor lower δ18O values, and the PC1 δ18O exhibit significant positive correlations with VPD across the entire Tropical Andes and the northern portion of the Amazon basin, and as well as outgoing longwave radiation across the southern Tropical Andes and part of the Amazon basin. The close relationships between the regional signals from our δ18O tree-ring network with the previously mentioned parameters, highlight the potential to develop future hydroclimatic-related reconstructions with these δ18O records to assess climate variability and change across the Tropical Andes. • A δ18O Polylepis tarapacana tree-ring network is developed in the Southern Tropical Andes. • The δ18O chronologies highlight a strong common signal. • Hydroclimate variability controls δ18O P. tarapacana content across our network. • Tropical atmospheric and circulation patterns show a close relation with δ18O tree-rings. [ABSTRACT FROM AUTHOR]

Poor drought tolerance of European beech trees raised concerns in Europe. We hypothesized that beech could show an opposite physiological response to the same level of climatic drought with change in edaphic drought. We performed a combined analysis of δ13C and δ18O in tree rings to reveal retrospective temporal physiological responses of trees to drought. The edaphic drought was assessed by quantifying the capacity of soil to store water in plots (classified as "dry" and "less-dry") near the drought limit of the species in three near-natural oak-beech ecotones in Germany and Switzerland. Neighbourhood competition was quantified. A climatic drought index was calculated from meteorological records and related to the δ13C and δ18O values of the trees. Trees from dry plots showed a higher response to drought and climatic dependency than less-dry plots. Neighbourhood competetion increased δ18O values significantly. Dual isotope analysis shows a tendency of greater stomatal resistance in dry plots and higher stomatal conductance in less-dry plots. We conclude that beech trees belonging to the same population under changing soil water availability can show different physiological responses under climatic drought stress. Our finding indicates the high plasticity of the beech trees to survive drought stress with changing site conditions. [ABSTRACT FROM AUTHOR]

Abstract: We explore the 20th century climate sensitivity of annually resolved carbon and oxygen isotope ratios in five Pinus uncinata individuals from the upper treeline in ~2400m asl of the Spanish Pyrenees. Time series of δ13C and δ18O are calibrated against temperature, precipitation, and drought indices over the period 1901–2009. Negative correlations of δ13C with summer precipitation and drought indices, as well as positive correlations with summer temperatures, confirm previous evidence from similar habitats in the Pyrenees. In contrast to this summer climate signal in the carbon isotopes, the δ18O record reveals mainly negative correlations with spring precipitation and drought. We explore the coherence between δ13C and δ18O time series derived from individual trees and assess the influence of widely applied δ13C correction procedures on the climate signal strength. Spatial correlation patterns and decomposition of the time series into high- and low-frequency components are used to develop a calibration setup for carbon and oxygen isotope ratios, which will improve long-term climate reconstructions in a region, where classical tree-ring width and density data are limited. [Copyright &y& Elsevier]

UV-laser-based microscopic systems were utilized to dissect and sample organic tissue for stable isotope measurements from thin wood cross-sections., We tested UV-laser-based microscopic tissue dissection in practice for high-resolution isotopic analyses (δ13C/δ18O) on thin cross-sections from different tree species. The method allows serial isolation of tissue of any shape and from millimetre down to micrometre scales. On-screen pre-defined areas of interest were automatically dissected and collected for mass spectrometric analysis., Three examples of high-resolution isotopic analyses revealed that: in comparison to δ13C of xylem cells, woody ray parenchyma of deciduous trees have the same year-to-year variability, but reveal offsets that are opposite in sign depending on whether wholewood or cellulose is considered; high-resolution tree-ring δ18O profiles of Indonesian teak reflect monsoonal rainfall patterns and are sensitive to rainfall extremes caused by ENSO; and seasonal moisture signals in intra-tree-ring δ18O of white pine are weighted by nonlinear intra-annual growth dynamics., The applications demonstrate that the use of UV-laser-based microscopic dissection allows for sampling plant tissue at ultrahigh resolution and unprecedented precision. This new technique facilitates sampling for stable isotope analysis of anatomical plant traits like combined tree eco-physiological, wood anatomical and dendroclimatological studies. [ABSTRACT FROM AUTHOR]

The response of evapotranspiration to anthropogenic warming is of critical importance to the water and carbon cycle. Conflicting observations about changes of evapotranspiration stem mostly from the brevity of observations in time and space as well as a high degree of internal variability. Here we present the first gridded reconstruction of the European summer vapour pressure deficit (VPD) for the past four centuries. The gridded reconstruction is based on 26 European tree-ring oxygen isotope records and is performed using a Random Forest approach. Based on our reconstruction, we show that from the mid- 1700s a trend towards higher VPD occurred in Central Europe and the Mediterranean region which is based on the simultaneous increase in temperature and decrease in precipitation. This increasing VPD trend continues throughout the observational period and recent times. Climate model projections show this increase in VPD for the Mediterranean region continuing until the end of the 21st century, whereby the extent depends on the amount of greenhouse gas emissions. In contrast, projected VPD in North and Central Europe shows a tendency towards higher VPD only in the highest emission scenario. The reconstructed, observed and modelled VPD for past present and future is available here: https://doi.org/10.5281/zenodo.5958837 (Balting, D. F. et al., 2022). [ABSTRACT FROM AUTHOR]

Abstract: δ 13C and δ 18O of tree rings contain time integrated information about the environmental conditions weighted by seasonal growth dynamics and are well established as sources of palaeoclimatic and ecophysiological data. Annually resolved isotope chronologies are frequently produced by pooling dated growth rings from several trees prior to the isotopic analyses. This procedure has the advantage of saving time and resources, but precludes from defining the isotopic error or statistical uncertainty related to the inter-tree variability. Up to now only a few studies have compared isotope series from pooled tree rings with isotopic measurements from individual trees. We tested whether or not the δ 13C and the δ 18O chronologies derived from pooled and from individual tree rings display significant differences at two locations from the Iberian Peninsula to assess advantages and constraints of both methodologies. The comparisons along the period 1900–2003 reveal a good agreement between pooled chronologies and the two mean master series which were created by averaging raw individual values (Mean) or by generating a mass calibrated mean (MassC). In most of the cases, pooled chronologies show high synchronicity with averaged individual samples at interannual scale but some differences also show up especially when comparing δ 18O decadal to multi-decadal variations. Moreover, differences in the first order autocorrelation among individuals may be obscured by pooling strategies. The lack of replication of pooled chronologies prevents detection of a bias due to a higher mass contribution of one sample but uncertainties associated with the analytical process itself, as sample inhomogeneity, seems to account for the observed differences. [Copyright &y& Elsevier]

This dendroclimatological research is based on two close pine forests ( Pinus sylvestris and Pinus uncinata) located at the Northern Iberian System (Spain), and three tree-ring variables (ring widths, δ13C and δ18O). The climate-tree growth system was assessed at local and regional scales using three climate datasets. Calibration of tree-ring records with climate showed a diversity of information recorded in the different variables, such as a general response to temperature and precipitation of current growing period, and an important contribution of previous year conditions understood as the use of food reserves. The analysis of the stability of climate-tree growth relationships throughout the twentieth century showed a shift of those climatic variables to which trees responded and results suggested an enhancement of reserve use on current tree growth. The results obtained in this research made clear a physiological adaptation of trees to changing climate. The results provided hints that the recent warming coupled to slight precipitation decay are forcing growth of studied trees to a higher stress status and to a higher climate-growth synchronisation. These instabilities also have implications on future dendroclimatic reconstructions performed with trees growing under changing environments. [ABSTRACT FROM AUTHOR]

Australian climate-proxy reconstructions based on tree rings from tropical and subtropical forests have not been achieved so far due to the rarity of species producing anatomically distinct annual growth rings. Our study identifies the Australian Red Cedar ( Toona ciliata) as one of the most promising tree species for tree-ring research in Australasia because this species exhibits distinct annual tree rings, a prerequisite for high quality tropical dendroclimatology. Based on these preliminary studies, we were able, for the first time in subtropical and tropical Australia, to develop a statistically robust, precisely dated and annually resolved chronology back to AD1854. We show that the variability in ring widths of T. ciliata is mainly dependent on annual precipitation. The developed proxy data series contains both high- and low-frequency climate signals which can be associated with the El Niño Southern Oscillation (ENSO) and the Interdecadal Pacific Oscillation (IPO). A comparison of different data sets (Brisbane precipitation, tree rings, coral luminescence record from the Great Barrier Reef, ENSO and IPO) revealed non-stationary correlation patterns throughout the twentieth century but little instability between the new tree-ring chronology and Brisbane precipitation. [ABSTRACT FROM AUTHOR]

Abstract: In tropical Australia, palaeoclimatic proxies derived from tree rings are sought after sources for reconstructing climate variations. However, dendroclimatology has not been widely applied in tropical forests and even less so in the Australian tropics due to the extreme rarity of species producing anatomically distinct annual growth rings. Furthermore, most Australian tree species exhibit rather strong opportunistic growth with non-annual growth zones that are less suitable for dendrochronology. Recent studies on the Australian Red Cedar (Toona ciliata) in the Upper Kangaroo Valley near Sydney revealed that tree-ring based climate reconstructions are feasible with this species. This study moved 2500 km further north and concentrated on the tropical stands of T. ciliata because it is one of the few deciduous tree species in tropical Australia likely characterised by a dormant period of the cambium and thus annual tree rings. Although dendroclimatological studies indicate that some Australian tree species are suitable for reconstructing climate patterns, a well replicated tree-ring record from Far North Queensland has not been developed until now. Tree cores of T. ciliata were developed into a 140-year tree-ring widths index chronology. The analyses showed that the ring-widths indices correlate with March–June precipitation as recorded at Kairi research station. March–June precipitation was reconstructed using the tree-ring data with 35% of the variance explained. The reconstructed series contains both high- and low-frequency climate signals. This suggests that growth of T. ciliata is influenced by climate phenomena of different wave lengths which can be associated with El Niño Southern Oscillation (ENSO) and Interdecadal Pacific Oscillation (IPO). [Copyright &y& Elsevier]

Abstract: In this study we have analyzed the variability of tree-ring widths and stable isotopes (δ 13C and δ 18O) of a single sessile oak tree (Quercus petraea (Matt.) Liebl.) since these parameters are critical in reconstructing the environment, particularly climatic conditions. Tree rings were separated into early- and latewood (EW t ; LW t ), tree ring (TR t ), and transfer tree ring (TTRt, the latter being the latewood plus the earlywood of the subsequent year. Mean sensitivity, simple correlation, partial correlation and autocorrelation analyses were applied to describe data and relationships. Although this research focused on a single tree, the results compared well with average site data. Widths and δ 18O values showed generally low autocorrelation for all tree-ring components, whereas δ 13C revealed highly significant autocorrelations for most tree-ring components. Mean sensitivity of the standardized values turned out to be high for δ 18O, marginally lower for width and the lowest for δ 13C. Correlation analyses have proven that the relationships within the tree-ring widths or within the isotope parameters are much stronger than across widths and isotope parameters. The study demonstrates the unique potential of all measured tree-ring data to be used as climate proxies. [Copyright &y& Elsevier]

Twentieth-century warming could lead to increases in the moisture-holding capacity of the atmosphere, altering the hydrological cycle and the characteristics of precipitation. Such changes in the global rate and distribution of precipitation may have a greater direct effect on human well-being and ecosystem dynamics than changes in temperature itself. Despite the co-variability of both of these climate variables, attention in long-term climate reconstruction has mainly concentrated on temperature changes. Here we present an annually resolved oxygen isotope record from tree-rings, providing a millennial-scale reconstruction of precipitation variability in the high mountains of northern Pakistan. The climatic signal originates mainly from winter precipitation, and is robust over ecologically different sites. Centennial-scale variations reveal dry conditions at the beginning of the past millennium and through the eighteenth and early nineteenth centuries, with precipitation increasing during the late nineteenth and the twentieth centuries to yield the wettest conditions of the past 1,000 years. Comparison with other long-term precipitation reconstructions indicates a large-scale intensification of the hydrological cycle coincident with the onset of industrialization and global warming, and the unprecedented amplitude argues for a human role. [ABSTRACT FROM AUTHOR]

Stable carbon and oxygen isotope ratios of raw pollen sampled from nine abundant tree species growing in natural habitats of central and northern Europe were investigated to understand the intra- and inter-specific variability of pollen-isotope values. All species yielded specific δ13Cpollen and δ18Opollen values and patterns, which can be ascribed to their physiology and habitat preferences. Broad-leaved trees flowering early in the year before leaf proliferation (Alnus glutinosa and Corylus avellana) exhibited on average 2.6‰ lower δ13Cpollen and 3.1‰ lower δ18Opollen values than broad-leaved and coniferous trees flowering during mid and late spring (Acer pseudoplatanus, Betula pendula, Carpinus betulus, Fagus sylvatica, Picea abies, Pinus sylvestris and Quercus robur). Mean species-specific δ13Cpollen values did not change markedly over time, whereas δ18Opollen values of two consecutive years were often statistically distinct. An intra-annual analysis of B. pendula and P. sylvestris pollen revealed increasing δ18Opollen values during the final weeks of pollen development. However, the δ13Cpollen values remained consistent throughout the pollen-maturation process. Detailed intra-individual analysis yielded circumferential and height-dependent variations within carbon and oxygen pollen-isotopes and the sampling position on a tree accounted for differences of up to 3.5‰ for δ13Cpollen and 2.1‰ for δ18Opollen. A comparison of isotope ranges from different geographic settings revealed gradients between maritime and continental as well as between high and low altitudinal study sites. The results of stepwise regression analysis demonstrated, that carbon and oxygen pollen-isotopes also reflect local non-climate environmental conditions. A detailed understanding of isotope patterns and ranges in modern pollen is necessary to enhance the accuracy of palaeoclimate investigations on δ13C and δ18O of fossil pollen. Furthermore, pollen-isotope values are species-specific and the analysis of species growing during different phenophases may be valuable for palaeoweather reconstructions of different seasons. [ABSTRACT FROM AUTHOR]

Aim: The aim was to decipher Europe‐wide spatio‐temporal patterns of forest growth dynamics and their associations with carbon isotope fractionation processes inferred from tree rings as modulated by climate warming. Location: Europe and North Africa (30‒70° N, 10° W‒35° E). Time period: 1901‒2003. Major taxa studied: Temperate and Euro‐Siberian trees. Methods: We characterize changes in the relationship between tree growth and carbon isotope fractionation over the 20th century using a European network consisting of 20 site chronologies. Using indexed tree‐ring widths (TRWi), we assess shifts in the temporal coherence of radial growth across sites (synchrony) for five forest ecosystems (Atlantic, boreal, cold continental, Mediterranean and temperate). We also examine whether TRWi shows variable coupling with leaf‐level gas exchange, inferred from indexed carbon isotope discrimination of tree‐ring cellulose (Δ13Ci). Results: We find spatial autocorrelation for TRWi and Δ13Ci extending over a maximum of 1,000 km among forest stands. However, growth synchrony is not uniform across Europe, but increases along a latitudinal gradient concurrent with decreasing temperature and evapotranspiration. Latitudinal relationships between TRWi and Δ13Ci (changing from negative to positive southwards) point to drought impairing carbon uptake via stomatal regulation for water saving occurring at forests below 60° N in continental Europe. An increase in forest growth synchrony over the 20th century together with increasingly positive relationships between TRWi and Δ13Ci indicate intensifying impacts of drought on tree performance. These effects are noticeable in drought‐prone biomes (Mediterranean, temperate and cold continental). Main conclusions: At the turn of this century, convergence in growth synchrony across European forest ecosystems is coupled with coordinated warming‐induced effects of drought on leaf physiology and tree growth spreading northwards. Such a tendency towards exacerbated moisture‐sensitive growth and physiology could override positive effects of enhanced leaf intercellular CO2 concentrations, possibly resulting in Europe‐wide declines of forest carbon gain in the coming decades. [ABSTRACT FROM AUTHOR]

Tree-rings are recorders of environmental signals and are therefore often used to reconstruct past environmental conditions. In this paper, we present four annually resolved, multi-centennial tree-ring isotope series from the southeastern Tibetan plateau. The investigation site, where juniper and spruce trees jointly occur, is one of the highest known tree-stands in the world. Tree ring cellulose oxygen (δ18O) and carbon (δ13C) isotopes were analyzed for a common period of 1685–2007 AD to investigate climate–isotope relationships. Therefore, various climate parameters from a local meteorological station and from the CRU 4.02 dataset were used. Tree-ring δ18O of both species revealed highly significant sensitivities with a high degree of coherence to hydroclimate variables during the growing season. The obtained δ18O–climate relationships can even be retained using a species mean. In contrast, the individual δ13C series indicated a weaker and non-uniform response to the tested variables. Underlying species-specific responses and adaptations to the long-term trend in atmospheric CO2 bias even after a trend correction identified dominant environmental factors triggering the tree-ring δ13C at our site. However, analysis of individual intrinsic water-use efficiency in juniper and spruce trees indicated a species-specific adaptation strategy to climate change. [ABSTRACT FROM AUTHOR]

Scots pine (Pinus sylvestris L.) is a widely used tree species in European dendroclimatology studies due to its common distribution across much of the continent. Almost all studies find radial growth strongly related to summer temperature, a result reflecting site selection at high elevation/latitude environments where trees grow at their ecophysiological limits. Due to the amount of attention spent on these sites there is a geographical and seasonal bias in temperature reconstructions based upon tree‐ring proxies in Europe. To overcome the limited availability of tree‐ring data in temperate lowlands, we present a northern Poland ring‐width chronology developed from living and historic Scots pine material with a strong common growth signal going back to AD 1200. Investigations into climate‐growth relationships found year‐to‐year ring‐width variability to be more strongly correlated to cold season temperature (November to April) prior to the growing season than summer temperatures during tree‐ring formation. Based on this relationship it was possible to reconstruct cold season temperature conditions for the last 810 years. Spatial field correlations with gridded instrumental records indicated that the reconstruction provides relevant cold season temperature information across the land regions bordering the North Atlantic Ocean and Baltic Sea, lowlands and uplands of western and central Europe, and the eastern and central interior of Russia. Despite an unsuccessful attempt to find a stationary relationship with the North Atlantic Oscillation, comparisons with several cold season temperature reconstructions confirmed the long‐term connection between our reconstructed temperature series for northern Poland and the wider area. [ABSTRACT FROM AUTHOR]

Abstract: In forests, the increase in atmospheric CO2 concentrations (Ca) has been related to enhanced tree growth and intrinsic water‐use efficiency (iWUE). However, in drought‐prone areas such as the Mediterranean Basin, it is not yet clear to what extent this “fertilizing” effect may compensate for drought‐induced growth reduction. We investigated tree growth and physiological responses at five Scots pine (Pinus sylvestris L.) and five sessile oak (Quercus petraea (Matt.) Liebl.) sites located at their southernmost distribution limits in Europe for the period 1960–2012 using annually resolved tree‐ring width and δ13C data to track ecophysiological processes. Results indicated that all 10 natural stands significantly increased their leaf intercellular CO2 concentration (Ci), and consequently iWUE. Different trends in the theoretical gas‐exchange scenarios as a response to increasing Ca were found: generally, Ci tended to increase proportionally to Ca, except for trees at the driest sites in which Ci remained constant. Ci from the oak sites displaying higher water availability tended to increase at a comparable rate to Ca. Multiple linear models fitted at site level to predict basal area increment (BAI) using iWUE and climatic variables better explained tree growth in pines (31.9%–71.4%) than in oak stands (15.8%–46.8%). iWUE was negatively linked to pine growth, whereas its effect on growth of oak differed across sites. Tree growth in the western and central oak stands was negatively related to iWUE, whereas BAI from the easternmost stand was positively associated with iWUE. Thus, some Q. petraea stands might have partially benefited from the “fertilizing” effect of rising Ca, whereas P. sylvestris stands due to their strict closure of stomata did not profit from increased iWUE and consequently showed in general growth reductions across sites. Additionally, the inter‐annual variability of BAI and iWUE displayed a geographical polarity in the Mediterranean. [ABSTRACT FROM AUTHOR]

Tree rings are natural archives that annually record distinct types of past climate variability depending on the parameters measured. Here, we use ring-width and stable isotopes in cellulose of trees from the northwestern Iberian Peninsula (IP) to understand regional summer hydroclimate over the last 400 years and the associated atmospheric patterns. Correlations between tree rings and climate data demonstrate that isotope signatures in the targeted Iberian pine forests are very sensitive to water availability during the summer period, and are mainly controlled by stomatal conductance. Non-linear methods based on extreme events analysis allow for capturing distinct seasonal climatic variability recorded by tree-ring parameters and asymmetric signals of the associated atmospheric features. Moreover, years with extreme high (low) values in the tree-ring records were characterised by coherent large-scale atmospheric circulation patterns with reduced (enhanced) moisture transport onto the northwestern IP. These analyses of extremes revealed that high/low proxy values do not necessarily correspond to mirror images in the atmospheric anomaly patterns, suggesting different drivers of these patterns and the corresponding signature recorded in the proxies. Regional hydroclimate features across the broader IP and western Europe during extreme wet/dry summers detected by the northwestern IP trees compare favourably to independent multicentury sea level pressure and drought reconstructions for Europe. Historical records also validate our findings that attribute non-linear moisture signals recorded by extreme tree-ring values to distinct large-scale atmospheric patterns and allow for 400-year reconstructions of the frequency of occurrence of extreme conditions in late spring and summer hydroclimate. [ABSTRACT FROM AUTHOR]

Drought is a key limiting factor for tree growth in the Mediterranean Basin. However, the variability in acclimation via xylem traits is largely unknown. We studied tree growth and vessel features of Quercus petraea (Matt.) Lieb. in five marginal stands across southern Europe. Tree-ring width (TRW), mean earlywood vessel area (MVA) and number of earlywood vessels (NV) as well as theoretical hydraulic conductivity (Kh) chronologies were developed for the period 1963-2012. Summer drought signals were consistent among TRW chronologies; however, climatic responses of vessel features differed considerably among sites. At the three xeric sites, previous year's summer drought had a negative effect on MVA and a positive effect on NV. In contrast, at the two mesic sites, current year's spring drought negatively affected NV, while exerting a positive influence on MVA. In both cases, Kh was not altered by this xylem adjustment. All variables revealed identical east-west geographical patterns in growth and anatomical features. Sessile oak copes with drought in different ways: at xeric sites and after unfavourable previous summer conditions more but smaller vessels are built, lowering vulnerability to cavitation, whereas at mesic sites, dry springs partly lead to tree-rings with wider but fewer vessels. The variability of vessel-related features displays a similar geographical dipole in the Mediterranean Basin previously described for tree growth by other studies. [ABSTRACT FROM AUTHOR]

The warming trend at the end of the last glacial was disrupted by rapid cooling clearly identified in Greenland (Greenland Stadial 1 or GS-1) and Europe (Younger Dryas Stadial or YD). This reversal to glacial-like conditions is one of the best known examples of abrupt change but the exact timing and global spatial extent remain uncertain. Whilst the wider Atlantic region has a network of high-resolution proxy records spanning GS-1, the Pacific Ocean suffers from a scarcity of sub-decadally resolved sequences. Here we report the results from an investigation into a tree-ring chronology from northern New Zealand aimed at addressing the paucity of data. The conifer tree species kauri ( Agathis australis ) is known from contemporary studies to be sensitive to regional climate changes. An analysis of a ‘historic’ 452-year kauri chronology confirms a tropical-Pacific teleconnection via the El Niño – Southern Oscillation (ENSO). We then focus our study on a 1010-year sub-fossil kauri chronology that has been precisely dated by comprehensive radiocarbon dating and contains a striking ring-width downturn between ∼12,500 and 12,380 cal BP within GS-1. Wavelet analysis shows a marked increase in ENSO-like periodicities occurring after the downturn event. Comparison to low- and mid-latitude Pacific records suggests a coherency with ENSO and Southern Hemisphere atmospheric circulation change during this period. The driver(s) for this climate event remain unclear but may be related to solar changes that subsequently led to establishment and/or increased expression of ENSO across the mid-latitudes of the Pacific, seemingly independent of the Atlantic and polar regions. [ABSTRACT FROM AUTHOR]

A tree ring oxygen isotope (δ 18 O TR ) chronology developed from one species ( Cedrela odorata ) growing in a single site has been shown to be a sensitive proxy for rainfall over the Amazon Basin, thus allowing reconstructions of precipitation in a region where meteorological records are short and scarce. Although these results suggest that there should be large-scale (> 100 km) spatial coherence of δ 18 O TR records in the Amazon, this has not been tested. Furthermore, it is of interest to investigate whether other, possibly longer-lived, species similarly record interannual variation of Amazon precipitation, and can be used to develop climate sensitive isotope chronologies. In this study, we measured δ 18 O in tree rings from seven lowland and one highland tree species from Bolivia. We found that cross-dating with δ 18 O TR gave more accurate tree ring dates than using ring width. Our “isotope cross-dating approach” is confirmed with radiocarbon “bomb-peak” dates, and has the potential to greatly facilitate development of δ 18 O TR records in the tropics, identify dating errors, and check annual ring formation in tropical trees. Six of the seven lowland species correlated significantly with C. odorata , showing that variation in δ 18 O TR has a coherent imprint across very different species, most likely arising from a dominant influence of source water δ 18 O on δ 18 O TR. In addition we show that δ 18 O TR series cohere over large distances, within and between species. Comparison of two C. odorata δ 18 O TR chronologies from sites several hundreds of kilometres apart showed a very strong correlation ( r = 0.80, p < 0.001, 1901–2001), and a significant (but weaker) relationship was found between lowland C. odorata trees and a Polylepis tarapacana tree growing in the distant Altiplano ( r = 0.39, p < 0.01, 1931–2001). This large-scale coherence of δ 18 O TR records is probably triggered by a strong spatial coherence in precipitation δ 18 O due to large-scale controls. These results highlight the strength of δ 18 O TR as a precipitation proxy, and open the way for temporal and spatial expansion of precipitation reconstructions in South America. [ABSTRACT FROM AUTHOR]

In the eastern Mediterranean in general and in Turkey in particular, temperature reconstructions based on tree rings have not been achieved so far. Furthermore, centennial-long chronologies of stable isotopes are generally also missing. Recent studies have identified the tree species Juniperus excelsa as one of the most promising tree species in Turkey for developing long climate sensitive stable carbon isotope chronologies because this species is long-living and thus has the ability to capture low-frequency climate signals. We were able to develop a statistically robust, precisely dated and annually resolved chronology back to AD 1125. We proved that variability of δC in tree rings of J. excelsa is mainly dependent on winter-to-spring temperatures (January-May). Low-frequency trends, which were associated with the medieval warm period and the little ice age, were identified in the winter-to-spring temperature reconstruction, however, the twentieth century warming trend found elsewhere could not be identified in our proxy record, nor was it found in the corresponding meteorological data used for our study. Comparisons with other northern-hemispherical proxy data showed that similar low-frequency signals are present until the beginning of the twentieth century when the other proxies derived from further north indicate a significant warming while the winter-to-spring temperature proxy from SW-Turkey does not. Correlation analyses including our temperature reconstruction and seven well-known climate indices suggest that various atmospheric oscillation patterns are capable of influencing the temperature variations in SW-Turkey. [ABSTRACT FROM AUTHOR]

Abstract: Climatic hazards, such as severe droughts and floods, affect extensive areas across monsoon Asia and can have profound impacts on the populations of that region. The area surrounding Indonesia, including large portions of the eastern Indian Ocean and Java Sea, plays a key role in the global climate system because of the enormous heat and moisture exchange that occurs between the ocean and atmosphere there. Here, we evaluate the influence of rainfall variability on multiple tree-ring parameters of teak (Tectona grandis) trees growing in a lowland rain forest in Central Java (Indonesia). We assess the potential of, annually resolved, tree-ring width, stable carbon (δ13C) and oxygen (δ18O) isotope records to improve our understanding of the Asian monsoon variability. Climate response analysis with regional, monthly rainfall data reveals that all three tree-ring parameters are significantly correlated to rainfall, albeit during different monsoon seasons. Precipitation in the beginning of the rainy season (Sep–Nov) is important for tree-ring width, confirming previous studies. Compared to ring width, the stable isotope records possess a higher degree of common signal, especially during portions of the peak rainy season (δ13C: Dec–May; δ18O: Nov–Feb) and are negatively correlated to rainfall. In addition, tree-ring δ18O also responds positively to peak dry season rainfall, although the δ18O rainy season signal is stronger and more time-stable. The correlations of opposite sign reflect the distinct seasonal contrast of the δ18O signatures in rainfall (18OPre) during the dry (18O-enriched rain) and rainy (18O-depleted rain) seasons. This difference in 18OPre signal reflects the combination of two signals in the annual tree-ring δ18O record. Highly resolved intra-annual δ18O isotope analyses suggest that the signals of dry and rainy season can be distinguished clearly. Thereby reconstructions can improve our understanding of variations and trends of the hydrological cycle over the Indonesian archipelago. [Copyright &y& Elsevier]

Tree-ring chronologies of Pinus sylvestris L. from latitudinal and altitudinal limits of the species distribution have been widely used for climate reconstructions, but there are many sites within the temperate climate zone, as is the case in northeastern Germany, at which there is little evidence of a clear climate signal in the chronologies. In this study, we developed long chronologies of several cell structure variables (e.g., average lumen area and cell wall thickness) from P. sylvestris growing in northeastern Germany and investigated the influence of climate on ring widths and cell structure variables. We found significant correlations between cell structure variables and temperature, and between tree-ring width and relative humidity and vapor pressure, respectively, enabling the development of robust reconstructions from temperate sites that have not yet been realized. Moreover, it has been shown that it may not be necessary to detrend chronologies of cell structure variables and thus low-frequency climate signals may be retrieved from longer cell structure chronologies. The relatively extensive resource of archaeological material of P. sylvestris covering approximately the last millennium may now be useful for climate reconstructions in northeastern Germany and other sites in the temperate climate zone. [ABSTRACT FROM PUBLISHER]

Aging in trees implies a progressive reduction in the growth rate, related to a shortening of the growing period and changes in the photosynthetic capability and efficiency. These changes may continue during the old-growth life stages following the juvenile phase and are reflected in tree-ring properties such as growth increment, density or stable isotopes. We studied possible climate age effects in time series of several tree-ring parameters (ring width, wood density and stable carbon and oxygen isotopes) of mature individuals from two age groups of Pinus uncinata and P. nigra at two locations in Spain. The aim was to test whether age differences in trees in the old-growth life stages could lead to diverging climate responses. The results show some differences in response to climate between age groups at a monthly level, but most of these divergences are not significant for seasonal climate variables. Regardless of the age group, the main limiting climate factors constrained tree growth equally. Although our findings do not support the idea of an age-dependent response to climate that may lead to inaccurate climate reconstructions, further studies using tree-ring density and stable isotope series are urgently needed to verify the current results. [ABSTRACT FROM AUTHOR]

We investigated the tree growth and physiological response of five pine forest stands in relation to changes in atmospheric CO concentration ( c) and climate in the Iberian Peninsula using annually resolved width and δC tree-ring chronologies since 1600. C discrimination (Δ≈ c/ c), leaf intercellular CO concentration ( c) and intrinsic water-use efficiency (iWUE) were inferred from δC values. The most pronounced changes were observed during the second half of the 20th century, and differed between stands. Three sites kept a constant c/ c ratio, leading to significant c and iWUE increases (active response to c); whereas a significant increase in c/ c resulted in the lowest iWUE increase of all stands at a relict Pinus uncinata forest site (passive response to c). A significant decrease in c/ c led to the greatest iWUE improvement at the northwestern site. We tested the climatic signal strength registered in the δC series after removing the low-frequency trends due to the physiological responses to increasing c. We found stronger correlations with temperature during the growing season, demonstrating that the physiological response to c changes modulated δC and masked the climate signal. Since 1970 higher δC values revealed iWUE improvements at all the sites exceeding values expected by an active response to the c increase alone. These patterns were related to upward trends in temperatures, indicating that other factors are reinforcing stomatal closure in these forests. Narrower rings during the second half of the 20th century than in previous centuries were observed at four sites and after 1970 at all sites, providing no evidence for a possible CO'fertilization' effect on growth. The iWUE improvements found for all the forests, reflecting both a c rise and warmer conditions, seem to be insufficient to compensate for the negative effects of the increasing water limitation on growth. [ABSTRACT FROM AUTHOR]

Abstract: We present one millennium-long (1171-year), and three 100 year long annually resolved δ13C tree-ring chronologies from ecologically varying Juniperus stands in the Karakorum Mountains (northern Pakistan), and evaluate their response to climatic and atmospheric CO2 changes. All δ13C records show a gradual decrease since the beginning of the 19th century, which is commonly associated with a depletion of atmospheric δ13C due to fossil fuel burning. Climate calibration of high-frequency δ13C variations indicates a pronounced summer temperature signal for all sites. The low-frequency component of the same records, however, deviates from long-term temperature trends, even after correction for changes in anthropogenic CO2. We hypothesize that these high-elevation trees show a response to both climate and elevated atmospheric CO2 concentration and the latter might explain the offset with target temperature data. We applied several corrections to tree-ring δ13C records, considering a range of potential CO2 discrimination changes over the past 150 years and calculated the goodness of fit with the target via calibration/verification tests (R 2, residual trend, and Durbin–Watson statistics). These tests revealed that at our sites, carbon isotope fixation on longer timescales is affected by increasing atmospheric CO2 concentrations at a discrimination rate of about 0.012‰/ppmv. Although this statistically derived value may be site related, our findings have implications for the interpretation of any long-term trends in climate reconstructions using tree-ring δ13C, as we demonstrate with our millennium-long δ13C Karakorum record. While we find indications for warmth during the Medieval Warm Period (higher than today’s mean summer temperature), we also show that the low-frequency temperature pattern critically depends on the correction applied. Patterns of long-term climate variation, including the Medieval Warm Period, the Little Ice Age, and 20th century warmth are most similar to existing evidence when a strong influence of increased atmospheric CO2 on plant physiology is assumed. [Copyright &y& Elsevier]

The analysis of δ13C and δ18O in tree-ring archives offers retrospective insights into environmental conditions and ecophysiological processes. While photosynthetic carbon isotope discrimination and evaporative oxygen isotope enrichment are well understood, we lack information on how the isotope signal is altered by downstream metabolic processes. In Pinus sylvestris, we traced the isotopic signals from their origin in the leaf water ( δ18O) or the newly assimilated carbon ( δ13C), via phloem sugars to the tree-ring, over a time-scale that ranges from hours to a growing season. Seasonally, variable 13C enrichment of sugars related to phloem loading and transport did lead to uncoupling between δ13C in the tree-ring, and the ci/ ca ratio at the leaf level. In contrast, the oxygen isotope signal was transferred from the leaf water to the tree-ring with an expected enrichment of 27‰, with time-lags of approximately 2 weeks and with a 40% exchange between organic oxygen and xylem water oxygen during cellulose synthesis. This integrated overview of the fate of carbon and oxygen isotope signals within the model tree species P. sylvestris provides a novel physiological basis for the interpretation of δ13C and δ18O in tree-ring ecology. [ABSTRACT FROM AUTHOR]

This paper examines tree-ring width and δ13C chronologies from a network of five Iberian pine forests to determine their sensitivity to climate variability under different site conditions. Interseries comparisons revealed better and more homogenous agreement among δ13C records than among tree-ring width series of the different sites. This suggests that δ13C ratios may preferentially record large-scale climatic signals, whereas ring-width variations may reflect more local factors. A negative relationship was found between ring-width and δ13C. As inferred from response function analyses, ring-width and δ13C showed significant relationships with climate. The analyses of different sites and species revealed unshared tree-ring width responses to summer temperature and precipitation, whereas all δ13C series were highly sensitive to current year summer precipitation and, to a lesser extent, to current summer temperature. A strong summer precipitation signal seems to dominate the δ13C of trees growing under Mediterranean climate, even when the mean climatic site conditions do not indicate distinct summer drought. Therefore, δ13C values reflect precipitation variability during the summer season better than tree-ring widths. This demonstrates that δ13C from tree-rings can be a very useful tool for climatic reconstruction in the Mediterranean region, especially when climate-growth relationships are weak. [ABSTRACT FROM AUTHOR]

• Evaluating trees' responses to climate change can be challenging. • We propose the use of correlation networks to aid the integration of wood traits. • Trees coordinate carbon and water related traits along a tropical climate gradient. • Correlation networks revealed trade-offs that reflects carbon metabolic pathways. • Cell-wall xylose and mannose are central in trees responses to climate variability. Tropical forests uptake more atmospheric CO 2 and transpire more water than any other forest in the world and are critical components of the global carbon and hydrological cycles. Both cycles depend to a great extent on the carbon and water balance of individual trees. Such adjustments are usually evaluated through well-established and newly-emerging traits but integrating them for a systemic understanding of trees' responses to climate change can be challenging. We propose using complex correlation networks to integrate and understand how trees coordinate water- and carbon-related traits under changing climate conditions. We built a correlation network based on 20 traits measured in the wood of Hymenaea courbaril (Leguminosae) trees, a species known for its extreme anisohydric water-use strategy, sampled along a climate gradient in Southeastern Brazil. Intercellular to ambient CO 2 concentrations ratio (c i /c a , estimated from tree-ring δ13C) is a central network trait for being coordinated with several hydraulic and carbon allocation traits. Trees of H. courbaril coordinate these traits along the climate gradient, favoring high c i /c a under warm and dry conditions. A high c i /c a is only possible through a consistent water supply provided by wider vessels together with the investment on soluble sugars, at the detriment of starch, likely for hydraulic maintenance. Trees also favor heat resistance by investing in cell-wall xylose, another central network trait, from xyloglucans and xylans, at the expense of mannose from glucomannans. Such trade-offs within, and between, structural and non-structural carbon allocation reflect well-known metabolic pathways in plants. In summary, this systemic approach confirms previously reported patterns on leaf physiology, stem hydraulics and carbon adjustments while bringing to light the previously unreported role of cell-wall composition and its fine adjustments to cope with climate change. [ABSTRACT FROM AUTHOR]

Abstract: We present the first and longest (413 years) dataset on stable carbon isotope ratios in tree-ring cellulose (δ 13C), tree-ring width (TRW), and maximum latewood density (MXD) obtained from larch trees growing on permafrost under continental climate in the Suntar Khayata mountain ridge in Eastern Siberia (Russia). With this first study we calibrate tree-ring parameters against climate quantities, and based on these results assess the potential added value of MXD and especially of δ 13C complementing TRW analysis for future climate reconstruction purposes. δ 13C chronologies were corrected for human induced changes in atmospheric CO2 since AD 1800. Two different approaches were compared i) a correction referring merely to the decline in atmospheric δ 13C (δ 13Catm) and ii) a correction additionally accounting for the increase in atmospheric partial pressure of CO2. δ 13C chronologies are characterized by strong signal strength with only 4 trees representing the population signal at the site (mean inter-series correlation=0.71 and EPS=0.90). δ 13C variation shows low similarity to TRW and MXD, while correlation between TRW and MXD is highly significant. Correlation analysis of tree-ring parameters with gridded instrumental data (Climate Research Unit, CRU TS 2.1) over the AD 1929–2000 calibration period demonstrates that TRW and MXD react as reported from other sites at cold and humid northern latitudes: precipitation plays no significant role, but strong dependencies on monthly mean, maximum and minimum temperatures, particularly of the current summer (June to August), are found (up to r =0.60, p <0.001). Combining instrumental data to a summer season mean (JJA) and TRW and MXD to a growth parameter mean (TRW+MXD), clearly shows the importance of the number of frost days and minimum temperatures during summer (r =0.67, p <0.001) to dominate tree growth and highlights the potential for climate reconstruction. Carbon isotope fixation in tree rings is obviously less controlled by temperature variables. In particular, the frost days and minimum temperature have a much smaller influence on δ 13C than on tree growth. δ 13C strongly reacts to current-year July precipitation (r =−0.44, p <0.05) and June–July maximum temperature (r =0.46, p <0.001). All significant (p <0.05) correlation coefficients are higher when using the corrected δ 13C chronology considering an additional plant physiological response on increasing atmospheric CO2 concentration, than using the chronology corrected for δ 13Catm changes alone. Spatial distribution of correlations between tree-ring data and climate variables for Eastern Siberia indicates that the summer temperature regime in the studied region is mostly influenced by Arctic air masses, but precipitation in July seems to be brought out from the Pacific region. Both the combined TRW+MXD record and the δ 13С record revealed a high reconstruction potential for summer temperature and precipitation, respectively, particularly on decadal and longer-term scales. [Copyright &y& Elsevier]

A number of emerging studies use stable oxygen and carbon isotopes from tree rings to reconstruct climate variables such as temperature and precipitation. In the mid latitudes, these key variables are essentially determined by the prevailing large scale atmospheric circulation which can be expressed via indices such as the North Atlantic Oscillation (NAO). However, only a few studies have investigated the relation between stable isotopes from trees and large-scale atmospheric circulation. Here we present ongoing research on the link between large scale atmospheric circulation and stable isotopes based on the European tree ring isotope network ISONET. This network contains stable oxygen and carbon isotope from 26 different sites in Europe over the last 400 years. In this study, empirical orthogonal functions (EOFs) were used to extract the key patterns and the climate information in this dataset. We focus on the first two EOFs of carbon and oxygen stable isotopes. Finally we use correlation and composite analysis in combination with gridded climate datasets, such as climate reanalysis and reconstructions, to assess the large scale summer climate conditions during the last 400 years.Strong links are identified between the investigated stable isotopes and the leading EOF of North Atlantic–European summer mean sea level pressure with its corresponding climate extremes. The frequency of extremes shows an increase from the 17th to the 20th century which may be attributed to the footprint of the Maunder Minimum and the Little Ice Age. In addition to the identified summer signal, a significant relation between oxygen isotopes and winter NAO is found. We propose that this mechanism works via precipitation in winter which subsequently modulates the soil moisture and water storage at the beginning of the growth season. Overall, the results highlight the potential of stable isotopes as an indicator for large-scale atmospheric conditions over Europe. [ABSTRACT FROM AUTHOR]

Developing long-term chronologies of tree-ring anatomical features to evaluate climatic relationships within species might serve as an annual proxy to explore and elucidate the climatic drivers affecting xylem differentiation. Pinus leucodermis response to climate was examined by analyzing vertical xylem resin ducts in wood growing at high elevation in the Apennines of peninsular Southern Italy. Early- and latewood tree-ring resin duct chronologies, spanning the 1804–2010 time period, were constructed. We analyzed the relationships between resin duct chronologies and climate over the last century using correlation and response function analyses. Overall, results showed that ring width and resin duct relationships differed between early- and latewood, which indicated conditions affecting growth were not associated with resin duct production. Results also revealed differential responses to climate between early- and latewood resin duct chronologies. A notable observation was a positive and stable relationship between latewood resin duct number chronology and July maximum temperature throughout the twentieth century. This result suggested resin ducts might be a suitable proxy to evaluate P. leucodermis response to climate in the study area.Developing long-term chronologies of tree-ring anatomical features to evaluate climatic relationships within species might serve as an annual proxy to explore and elucidate the climatic drivers affecting xylem differentiation. Pinus leucodermis response to climate was examined by analyzing vertical xylem resin ducts in wood growing at high elevation in the Apennines of peninsular Southern Italy. Early- and latewood tree-ring resin duct chronologies, spanning the 1804–2010 time period, were constructed. We analyzed the relationships between resin duct chronologies and climate over the last century using correlation and response function analyses. Overall, results showed that ring width and resin duct relationships differed between early- and latewood, which indicated conditions affecting growth were not associated with resin duct production. Results also revealed differential responses to climate between early- and latewood resin duct chronologies. A notable observation was a positive and stable relationship between latewood resin duct number chronology and July maximum temperature throughout the twentieth century. This result suggested resin ducts might be a suitable proxy to evaluate P. leucodermis response to climate in the study area.Developing long-term chronologies of tree-ring anatomical features to evaluate climatic relationships within species might serve as an annual proxy to explore and elucidate the climatic drivers affecting xylem differentiation. Pinus leucodermis response to climate was examined by analyzing vertical xylem resin ducts in wood growing at high elevation in the Apennines of peninsular Southern Italy. Early- and latewood tree-ring resin duct chronologies, spanning the 1804–2010 time period, were constructed. We analyzed the relationships between resin duct chronologies and climate over the last century using correlation and response function analyses. Overall, results showed that ring width and resin duct relationships differed between early- and latewood, which indicated conditions affecting growth were not associated with resin duct production. Results also revealed differential responses to climate between early- and latewood resin duct chronologies. A notable observation was a positive and stable relationship between latewood resin duct number chronology and July maximum temperature throughout the twentieth century. This result suggested resin ducts might be a suitable proxy to evaluate P. leucodermis response to climate in the study area.Developing long-term chronologies of tree-ring anatomical features to evaluate climatic relationships within species might serve as an annual proxy to explore and elucidate the climatic drivers affecting xylem differentiation. Pinus leucodermis response to climate was examined by analyzing vertical xylem resin ducts in wood growing at high elevation in the Apennines of peninsular Southern Italy. Early- and latewood tree-ring resin duct chronologies, spanning the 1804–2010 time period, were constructed. We analyzed the relationships between resin duct chronologies and climate over the last century using correlation and response function analyses. Overall, results showed that ring width and resin duct relationships differed between early- and latewood, which indicated conditions affecting growth were not associated with resin duct production. Results also revealed differential responses to climate between early- and latewood resin duct chronologies. A notable observation was a positive and stable relationship between latewood resin duct number chronology and July maximum temperature throughout the twentieth century. This result suggested resin ducts might be a suitable proxy to evaluate P. leucodermis response to climate in the study area.Developing long-term chronologies of tree-ring anatomical features to evaluate climatic relationships within species might serve as an annual proxy to explore and elucidate the climatic drivers affecting xylem differentiation. Pinus leucodermis response to climate was examined by analyzing vertical xylem resin ducts in wood growing at high elevation in the Apennines of peninsular Southern Italy. Early- and latewood tree-ring resin duct chronologies, spanning the 1804–2010 time period, were constructed. We analyzed the relationships between resin duct chronologies and climate over the last century using correlation and response function analyses. Overall, results showed that ring width and resin duct relationships differed between early- and latewood, which indicated conditions affecting growth were not associated with resin duct production. Results also revealed differential responses to climate between early- and latewood resin duct chronologies. A notable observation was a positive and stable relationship between latewood resin duct number chronology and July maximum temperature throughout the twentieth century. This result suggested resin ducts might be a suitable proxy to evaluate P. leucodermis response to climate in the study area. [ABSTRACT FROM AUTHOR]