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Surficial application of waste glycerol (WG) for enhanced bioremediation was tested in situ at an old military range site to address hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) contaminated groundwater. This treatment was effective in inducing strong reducing conditions (range: −4 to −205 mV) and increasing the concentrations of organic carbon (from 10 to 729 mg/L) and fatty acids (from 0 to 940 mg/L) concomitantly with a decrease in RDX concentrations (range: 17 to 143 μg/L) to below detection limits (0.1 μg/L) in 2 of the 3 monitoring wells (MWs) evaluated. None of these changes were observed in the control MW. RDX disappeared without the detection of any common anaerobic nitroso degradation intermediates, with the exception of one MW where the concentration of organics did not significantly increase (range: 10 to 20 mg/L), suggesting the conditions were not favourable for biodegradation. Ecotoxicological analysis suggested that the use of WG may have some dose-related deleterious effects on different soil and aquatic receptors. Analysis of the microbial community composition, using 16S rRNA gene amplicon sequences, which provided insight into whether the process design had selected for and stimulated the optimal microbial populations, indicated co-existence of numerous Operational Taxonomic Units (OTUs) belonging to groups known to be capable of RDX degradation under anaerobic conditions, with a positive link between Geobacter spp. enrichment and the presence of RDX nitroso metabolites. Overall, the results from this field test show that this treatment process can provide an effective long-term, semi-passive remediation option for RDX contaminated groundwater. Unlabelled Image • Waste glycerol (WG) was used in situ to treat RDX contaminated groundwater. • Surficially applied WG reached groundwater and induced strong reducing conditions. • Organic carbon and fatty acid concentrations increased while that of RDX decreased. • Dose-related deleterious effects of WG observed on soil and aquatic receptors • Amplicon sequence analysis revealed known RDX degrading taxa enriched by treatment. [ABSTRACT FROM AUTHOR]

Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) is a toxic, mobile groundwater contaminant common to military sites. Biodegradation of RDX is an alternative, cost effective and environmentally friendly remediation approach. The effects of carbon amendments (waste glycerol and cheese whey) used alone or with a potential electron shuttle (ammonium lignosulfonate) on RDX biodegradation were assessed. These substrates are readily available waste materials that can be used as nutrients to promote oxygen consumption, creating a more reducing environment. Nutrient amended batch assays were conducted using RDX spiked contaminated demolition range soil under anaerobic conditions. The amendments that improved RDX mineralization the most were subsequently tested in a scaled up repacked soil column study to verify if this strategy could be effectively implemented on-site. Microcosm results indicated that RDX mineralization by indigenous anaerobic microorganisms was enhanced the most by the low carbon amendment concentration. The use of ammonium lignosulfonate was not effective, exhibiting an inhibitory effect on RDX biodegradation that was stronger at higher concentrations. The soil column study showed that the low concentration of waste was the most promising treatment scenario. These results offer good prospects for the use of waste glycerol for in situ treatment of soils contaminated with energetic-materials, such as RDX. [ABSTRACT FROM PUBLISHER]