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Investigates the role of different transmembrane helices or hairpins in the binding of the drug ouabain. Transmembrane region involved in the specific interaction of the ouabain with enzymes responsible for active cation transport; Dephosphorylation of the phosphorylated enzymes.

Describes the role of the carbonyl group of glutamic acid-795 in gastric adenosinetriphosphatase (ATP) activities. Mutagenesis of glutamic acid-795; Generation of recombinant viruses; ATP phosphorylation; ATPase activity assay.

Examines the essential role of negatively charged glutamic acid of the gastric hydrogen/potassium ATPase in potassium binding. Phosphorylation of mutant cells; Ability of the mutant to form a phosphorylated intermediate; Inhibition of enzymes.

Reports on the preparation of a series of six different mutants of aspartic acid 804 present in transmembrane segment 6 of the rat sodium/potassium ATPase alpha1-subunit. Alteration in the affinity of the cation-binding pocket in favor of sodium instead of potassium; Preference of the enzyme for E[sub 1] conformation.

Examines the functional expression of sodium/potassium ATPase and hydrogen/potassium ATPase in insect cells. Structural features of the adenosine triphosphatase (ATP); Information about regions responsible for drug and cation specificity; Measurement of the ATP-phosphorylation levels.