1. <italic>Platr3</italic>/NUDT21/NF-κB Axis Mediates <italic>P. gingivalis</italic>-Suppressed Cementoblast Mineralization.
- Author
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Huang, Hantao, Ma, Li, Wang, Xiaoxuan, Huang, Xin, Wang, Huiyi, Peng, Yan, Xiao, Junhong, Liu, Heyu, Yang, Zhengkun, and Cao, Zhengguo
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X-ray computed microtomography , *LINCRNA , *FLUORESCENCE in situ hybridization , *MINERALIZATION , *SYNCRIP protein , *PERIAPICAL periodontitis , *SOX2 protein - Abstract
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Porphyromonas gingivalis (P. gingivalis ) is one of the major pathogens causing periodontitis and apical periodontitis (AP). Long noncoding RNA (lncRNA) can regulate cellular mineralization and inflammatory diseases. The aim of this study was to investigate the role and mechanism of lncRNA inP. gingivalis- stimulated cementoblast mineralization.In vivo , C57BL/6 mice were divided into the healthy, the AP, and AP +P. gingivalis groups (n = six mice per group). Micro computed tomography, immunohistochemistry staining, and fluorescence in situ hybridization were used to observe periapical tissue.In vitro , cementoblasts were treated with osteogenic medium orP. gingivalis. Pluripotency associated transcript 3 (Platr3), interleukin 1 beta (IL1B), and osteogenic markers were analyzed by quantitative real-time polymerase chain reaction and western blot. RNA pull-down and RNA immunoprecipitation assays were used to detect proteins that bind toPlatr3 . RNA sequencing was performed inPlatr3 -silenced cementoblasts.In vivo ,P. gingivalis promoted periapical tissue destruction and IL1B expression, but inhibitedPlatr3 expression.In vitro ,P. gingivalis facilitated IL1B expression (P < 0.001), whereas suppressed the expression of Platr3 (P < 0.001) and osteogenic markers (P < 0.01 or 0.001). In contrast,Platr3 overexpression alleviated the repressive effect ofP. gingivalis on cementoblast mineralization (P < 0.01 or 0.001). Furthermore,Platr3 bound to nudix hydrolase 21 (NUDT21) and regulated the nuclear factor-κB (NF-κB) signaling pathway. Knocking down NUDT21 suppressed osteogenic marker expression and activated the above signaling pathway. Collectively, the results elucidated thatPlatr3 mediatedP. gingivalis -suppressed cementoblast mineralization through the NF-κB signaling pathway by binding to NUDT21. [ABSTRACT FROM AUTHOR]- Published
- 2024
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