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Floating seaweed rafts are a surface-pelagic habitat that serve as substrates for benthic flora and fauna. Since 2008, Sargassum horneri clumps have periodically invaded the Korea Strait. In this study, the polymerase chain reaction-free small-organelles enriched metagenomics method was adopted to identify the species of epibiotic eukaryotes present in floating S. horneri fronds. A total of 185 species were identified, of which about 63% were previously undetected or unreported in Korean waters. The rafts harbored a diverse assemblage of eukaryotic species, including 39 Alveolata, 4 Archaeplastida, 95 Opisthokonts, 4 Rhizaria, and 43 Stramenopiles. Of these 185 taxa, 48 species were found at both Sargassum rafts collection stations and included 24 Stramenopiles, 17 Alveolata, and 7 Opisthokonts. Among these, the highest proportion (50%) of species was photo-autotrophic in basic trophic modes, while the proportion of phagotrophic, osmo- or saprotrophic, and parasitic modes were 43.8%, 4.2%, and 2.1%, respectively. This study demonstrates the contribution of floating Sargassum rafts as dispersal vectors that facilitate the spread of alien species. [ABSTRACT FROM AUTHOR]

To quantify the abundance of the harmful marine dinoflagellate Cochlodinium polykrikoides in natural seawaters, we developed an innovative procedure based on the droplet digital PCR (ddPCR) with C. polykrikoides-specific primers targeting the internal transcribed spacer (ITS). The abundance of C. polykrikoides was determined through the specific copy number of target DNA per cell in both culture and field samples. The average copy number per C. polykrikoides cell as determined via the ddPCR was 157 with using known cell numbers and a simplified protocol for preparing DNAs. C. polykrikoides cell numbers, in samples collected from different locations, calculated via ddPCR were in agreement with direct cell counts using a microscope. The ddPCR protocol was then used to measure the abundance of C. polykrikoides in nearshore and offshore waters of the southern coast of Korea. Practical application of this method demonstrated its usefulness in reducing time spent on analysis and for increasing estimate accuracy. [ABSTRACT FROM AUTHOR]

The present investigation of the chemical constituents of the stem barks of Ailanthus altissima has resulted in the isolation of six canthinone-type alkaloids, including a new compound, (R)-5-(1-hydroxyethyl)-canthine-6-one (1), and five known compounds (2–6). Moreover, four phenyl propanoids (7–10), two lignans (11 and 12), two triterpenoids (13 and 14) and a fatty acid (15) having previously known chemical structures were isolated during the same course of this study. The structure of the new compound was elucidated by physical (m.p., [α]D) and spectroscopic data (1H-NMR, 13C-NMR, 2D NMR, and HR-DART-MS) interpretation and its absolute configuration was determined by electronic circular dichroism (ECD) data and quantum chemical calculations. The inflammatory activities of the isolates were screened on lipopolysaccharide (LPS)-induced nitric oxide (NO), a proinflammatory mediator, in RAW 264.7 cells. Among these isolated compounds, six compounds exhibited significant inhibition of NO production, with IC50 values in the range of 5.92 ± 0.9 to 15.09 ± 1.8 μM. [ABSTRACT FROM AUTHOR]

Tropical Pacific Ocean warming has been separated into two modes based on the spatial distribution of the maximum sea surface temperature (SST) anomaly: an east Pacific warming (EPW) and a central Pacific warming (CPW). When combined with east Pacific cooling (EPC), these three regimes are shown to have different impacts on tropical cyclone (TC) activity over the North Pacific by differential modulation of both local thermodynamic factors and large-scale circulation patterns. In EPW years, the genesis and the track density of TCs tend to be enhanced over the southeastern part and suppressed in the northwestern part of the western Pacific by strong westerly wind shear. The extension of the monsoon trough and the weak wind shear over the central Pacific increases the likelihood of TC activity to the east of the climatological mean TC genesis location. In CPW years, the TC activity is shifted to the west and is extended through the northwestern part of the western Pacific. The westward shifting of CPW-induced heating moves the anomalous westerly wind and monsoon trough through the northwestern part of the western Pacific and provides a more favorable condition for TC landfall. The CPW, on the other hand, produces a large suppression of TC activity in the eastern Pacific basin. In EPC years, all of the variables investigated show almost a mirror image of the EPW. [ABSTRACT FROM AUTHOR]

The influence of ocean-atmosphere coupling on the simulation and prediction of the boreal winter Madden-Julian Oscillation (MJO) is examined using the Seoul National University coupled general circulation model (CGCM) and atmospheric-only model (AGCM). The AGCM is forced with daily SSTs interpolated from pentad mean CGCM SSTs. Forecast skill is examined using serial extended simulations spanning 26 different winter seasons with 30-day forecasts commencing every 5 days providing a total of 598 30-day simulations. By comparing both sets of experiments, which share the same atmospheric components, the influence of coupled ocean-atmosphere processes on the simulation and prediction of MJO can be studied. The mean MJO intensity possesses more realistic amplitude in the CGCM than in AGCM. In general, the ocean-atmosphere coupling acts to improve the simulation of the spatio-temporal evolution of the eastward propagating MJO and the phase relationship between convection (OLR) and SST over the equatorial Indian Ocean and the western Pacific. Both the CGCM and observations exhibit a near-quadrature relationship between OLR and SST, with the former lagging by about two pentads. However, the AGCM shows a less realistic phase relationship. As the initial conditions are the same in both models, the additional forcing by SST anomalies in the CGCM extends the prediction skill beyond that of the AGCM. To test the applicability of the CGCM to real-time prediction, we compute the Real-time Multivariate MJO (RMM) index and compared it with the index computed from observations. RMM1 (RMM2) falls away rapidly to 0.5 after 17-18 (15-16) days in the AGCM and 18-19 (16-17) days in the CGCM. The prediction skill is phase dependent in both the CGCM and AGCM. [ABSTRACT FROM AUTHOR]

The predictability of intraseasonal variation in the tropics is assessed in the present study by using various statistical and dynamical models with rigorous and fair measurements. For a fair comparison, the real-time multivariate Madden–Julian oscillation (MJO) (RMM) index, proposed by Wheeler and Hendon, is used as a predictand for all models. The statistical models include the models based on a multilinear regression, a wavelet analysis, and a singular spectrum analysis (SSA). The prediction limits (correlation skill of 0.5) of statistical models for RMM1 (RMM2) index are at days 16–17 (14–15) for the multiregression model, whereas they are at days 8–10 (9–12) for the wavelet- and SSA-based models. The poor predictability of the wavelet and SSA models is related to the tapering problem for a half-length of the time window before the initial condition. To assess the dynamical predictability, long-term serial prediction experiments with a prediction interval of every 5 days are carried out with Seoul National University (SNU) AGCM and coupled general circulation model (CGCM) for 26 (1980–2005) boreal winters. The prediction limits of RMM1 and RMM2 occur at around 20 days for both AGCM and CGCM. These results demonstrate that the skills of dynamical models used in this study are better than those of the three statistical predictions. The dynamical and statistical predictions are combined using a multimodel ensemble method. The combination provides a superior skill to any of the statistical and dynamical predictions, with a prediction limit of 22–24 days. The dependencies of prediction skill on the initial phase and amplitude of the MJO are also investigated. [ABSTRACT FROM AUTHOR]

Two distinctly different forms of tropical Pacific Ocean warming are shown to have substantially different impacts on the frequency and tracks of North Atlantic tropical cyclones. The eastern Pacific warming (EPW) is identical to that of the conventional El Niño, whereas the central Pacific warming (CPW) has maximum temperature anomalies located near the dateline. In contrast to EPW events, CPW episodes are associated with a greater-than-average frequency and increasing landfall potential along the Gulf of Mexico coast and Central America. Differences are shown to be associated with the modulation of vertical wind shear in the main development region forced by differential teleconnection patterns emanating from the Pacific. The CPW is more predictable than the EPW, potentially increasing the predictability of cyclones on seasonal time scales. [ABSTRACT FROM AUTHOR]

We report cellulose-based electro-active paper (EAPap) actuator formed by electrospinning. Cellulose is one of the most abundant natural polymers and has become an attractive smart material. To prepare electrospun nanofibrous cellulose membrane, the LiCl/N,N-dimethylacetamide (DMAc) solvent system was used. The presence of residual LiCl in electrospun membrane was confirmed by elemental analysis using energy dispersive X-ray spectroscopy. To induce orientation, the cellulose membrane was mechanically aligned in simple extension. FE-SEM was used to observe the morphology of the oriented electrospun membranes. The orientation and crystallinity of the cellulose membrane were examined by WAXD. The electro-active characteristic of the electrospun cellulose membrane was measured by the cyclic bending displacement test under different voltages. Aligning of the electrospun membrane was found necessary to render electro-active characteristic. [ABSTRACT FROM AUTHOR]

Catalytic drugs based on target-selective artificial proteases have been proposed as a new paradigm in drug design. Peptide-cleavage agents selective for pathogenic proteins of Alzheimer’s disease, type 2 diabetes mellitus or Parkinson’s disease have been prepared using the Co(III) aqua complex (Co(III)cyclen) of 1,4,7,10-tetraazacyclododecane as the catalytic center. In the present study, the Co(III) aqua complex (Co(III)oxacyclen) of 1-oxa-4,7,10-triazacyclododecane was examined in search of an improved catalytic center for peptide-cleavage agents. An X-ray crystallographic study of [Co(oxacyclen)(CO3)](ClO4), titration of Co(III)oxacyclen, and kinetic studies on the cleavage of albumin, γ-globulin, lysozyme, and myoglobin by Co(III)oxacyclen were carried out. Considerably higher proteolytic activity was observed for Co(III)oxacyclen in comparison with Co(III)cyclen, indicating that better target-selective artificial metalloproteases would be obtained using Co(III)oxacyclen as the catalytic center. The improved proteolytic activity was attributed to either steric effects or the increased Lewis acidity of the Co(III) center. The kinetic data also predicted that side effects due to the cleavage of nontarget proteins by a catalytic drug based on Co(III)oxacyclen would be insignificant. [ABSTRACT FROM AUTHOR]

The impact of ocean–atmosphere coupling on the simulation and prediction of the boreal summer intraseasonal oscillation (ISO) has been investigated by diagnosing 22-year retrospective forecasts using the Seoul National University coupled general circulation model (CGCM) and its atmospheric GCM (AGCM) forced with SSTs derived from the CGCM. Numerous studies have shown that the ocean–atmosphere coupling has a significant effect on the improvement of ISO simulation and prediction. Contrary to previous studies, this study shows similar results between CGCM and AGCM, not only in regard to the ISO simulation characteristics but also the predictability. The similarities between CGCM and AGCM include (1) the ISO intensity over the entire Asian-monsoon region; (2) the spatiotemporal evolution of the northward propagating ISO (NPISO); and (3) the potential and practical predictability. A notable difference between CGCM and AGCM is the phase relationship between precipitation and SST anomalies. The CGCM and observation exhibits a near-quadrature relationship between precipitation and SST, with the former lagging about two pentads. The AGCM shows a less realistic phase relationship. The similar structure and propagation characteristics of ISO between the CGCM and AGCM suggest that the internal atmospheric dynamics could be more essential to the ISO than the ocean–atmosphere interaction over the Indian monsoon region. [ABSTRACT FROM AUTHOR]

The influence of sea surface temperature (SST) on the simulation and predictability of the Madden–Julian oscillation (MJO) is examined using the Seoul National University atmospheric general circulation model (SNU AGCM). Forecast skill was examined using serial climate simulations spanning eight different winter seasons with 30-day forecasts commencing every 5 days, giving a total of 184 thirty-day simulations. The serial runs were repeated using prescribing observed SST with monthly, weekly, and daily temporal resolutions. The mean SST was the same for all cases so that differences between experiments result from the different temporal resolutions of the SST boundary forcing. It is shown that high temporal SST frequency acts to improve 1) the MJO activity of 200-hPa velocity potential field over the entire Asian monsoon region at all lead times; 2) the percentage of filtered variance of the two leading EOF modes that explain the eastward propagation of MJO; 3) the power of the wavenumber 1 eastward propagating mode; and 4) the forecast skill of MJO, maintaining it for longer periods. However, the MJO phase relationship between MJO convection and SST, as is often the case with many atmosphere-only models, although well simulated at the beginning of forecast period becomes distorted rapidly as the forecast lead time increases, even with the daily SST forcing case. Comparison of AGCM simulations with coupled GCM (CGCM) integrations shows that ocean–atmosphere coupling improves considerably the phase relationship between SST and convection. The CGCM results reinforce that the MJO is a coupled phenomenon and suggest strongly the need of the ocean–atmosphere coupled processes to extend predictability. [ABSTRACT FROM AUTHOR]

We report cellulose-based electro-active paper (EAPap) actuator formed by electrospinning. Cellulose is one of the most abundant natural polymers and has become an attractive smart material. To prepare electrospun nanofibrous cellulose membrane, the LiCl/N,N-dimethylacetamide (DMAc) solvent system was used. The presence of residual LiCl in electrospun membrane was confirmed by elemental analysis using energy dispersive X-ray spectroscopy. To induce orientation, the cellulose membrane was mechanically aligned in simple extension. FE-SEM was used to observe the morphology of the oriented electrospun membranes. The orientation and crystallinity of the cellulose membrane were examined by WAXD. The electro-active characteristic of the electrospun cellulose membrane was measured by the cyclic bending displacement test under different voltages. Aligning of the electrospun membrane was found necessary to render electro-active characteristic. [ABSTRACT FROM AUTHOR]

The reproducibility of boreal summer intraseasonal variability (ISV) and its interannual variation by dynamical models are assessed through diagnosing 21-year retrospective forecasts from ten state-of-the-art ocean–atmosphere coupled prediction models. To facilitate the assessment, we have defined the strength of ISV activity by the standard deviation of 20–90 days filtered precipitation during the boreal summer of each year. The observed climatological ISV activity exhibits its largest values over the western North Pacific and Indian monsoon regions. The notable interannual variation of ISV activity is found primarily over the western North Pacific in observation while most models have the largest variability over the central tropical Pacific and exhibit a wide range of variability in spatial patterns that are different from observation. Although the models have large systematic biases in spatial pattern of dominant variability, the leading EOF modes of the ISV activity in the models are closely linked to the models’ El Nino-Southern Oscillation (ENSO), which is a feature that resembles the observed ISV and ENSO relationship. The ENSO-induced easterly vertical shear anomalies in the western and central tropical Pacific, where the summer mean vertical wind shear is weak, result in ENSO-related changes of ISV activity in both observation and models. It is found that the principal components of the predicted dominant modes of ISV activity fluctuate in a very similar way with observed ones. The model biases in the dominant modes are systematic and related to the external SST forcing. Thus the statistical correction method of this study based on singular value decomposition is capable of removing a large portion of the systematic errors in the predicted spatial patterns. The 21-year-averaged pattern correlation skill increases from 0.25 to 0.65 over the entire Asian monsoon region after applying the bias correction method to the multi-model ensemble mean prediction. [ABSTRACT FROM AUTHOR]

While stationary organ cells are in continuous contact with neighboring cells, immune cells circulate throughout the body without an apparent requirement for cell-cell contact to persist in vivo. This study challenges current convention by demonstrating, both in vitro and in vivo, that innate immune NK cells can engage in homotypic NK-to-NK cell interactions for optimal survival, activation, and proliferation. Using a specialized cell-laden microwell approach, we discover that NK cells experiencing constant NK-to-NK contact exhibit a synergistic increase in activation status, cell proliferation, and anti-tumor function in response to IL-2 or IL-15. This effect is dependent on 2B4/CD48 ligation and an active cytoskeleton, resulting in amplification of IL-2 receptor signaling, enhanced CD122/CD132 colocalization, CD25 upregulation, and Stat3 activation. Conversely, 'orphan' NK cells demonstrate no such synergy and fail to persist. Therefore, our data uncover the existence of homotypic cell-to-cell communication among mobile innate lymphocytes, which promotes functional synergy within the cytokine-rich microenvironment. [ABSTRACT FROM AUTHOR]

The transcription factor PU.1 is often impaired in patients with acute myeloid leukemia (AML). Here, we used AML cells that already had low PU.1 levels and further inhibited PU.1 using either RNA interference or, to our knowledge, first-in-class small-molecule inhibitors of PU.1 that we developed specifically to allosterically interfere with PU.1-chromatin binding through interaction with the DNA minor groove that flanks PU.1-binding motifs. These small molecules of the heterocyclic diamidine family disrupted the interaction of PU.1 with target gene promoters and led to downregulation of canonical PU.1 transcriptional targets. shRNA or small-molecule inhibition of PU.1 in AML cells from either PU.1lo mutant mice or human patients with AML-inhibited cell growth and clonogenicity and induced apoptosis. In murine and human AML (xeno)transplantation models, treatment with our PU.1 inhibitors decreased tumor burden and resulted in increased survival. Thus, our study provides proof of concept that PU.1 inhibition has potential as a therapeutic strategy for the treatment of AML and for the development of small-molecule inhibitors of PU.1. [ABSTRACT FROM AUTHOR]

Cochlodinium polykrikoides is a red-tide forming dinoflagellate that causes significant worldwide impacts on aquaculture industries and the marine ecosystem. There have been extensive studies on managing and preventing C. polykrikoides blooms, but it has been difficult to identify an effective method to control the bloom development. There is also limited genome information on the molecular mechanisms involved in its various ecophysiology and metabolism processes. Thus, comprehensive genome information is required to better understand harmful algal blooms caused by C. polykrikoides. We estimated the C. polykrikoides genome size using flow cytometry, with detection of the fluorescence of DNA stained with propidium iodide (PI). The nuclear genome size of C. polykrikoides was 100.97 Gb, as calculated by comparing its mean fluorescence intensity (MFI) to the MFI of Mus musculus, which is 2.8 Gb. The exceptionally large genome size of C. polykrikoides might indicate its complex physiological and metabolic characteristics. Our optimized protocol for estimating the nuclear genome size of a dinoflagellate using flow cytometry with PI can be applied in studies of other marine organisms. [ABSTRACT FROM AUTHOR]

We report here the cases of two females with Graves' disease who developed insulin autoimmune syndrome after treatment with methimazole. The patients exhibited a sudden altered mental state after treatment with methimazole for approximately 4 weeks. Patients had hypoglycemia with serum glucose below 70 mg/dL, and laboratory findings showed both high levels of serum insu- lin and high titers of insulin autoantibodies. The two women had never been exposed to insulin or oral antidiabetic agents, and there was no evidence of insulinoma in imaging studies. After glucose loading, serum glucose, and total insulin levels increased abnormally. One of the patient was found to have HLA-DRB1*0406, which is known to be strongly associated with methima- zole-induced insulin autoimmune syndrome. After discontinuation of methimazole, hypoglycemic events disappeared within 1 month. Insulin autoantibody titer and insulin levels decreased within 5 months and there was no further development of hypogly- cemic events. We present these cases with a review of the relevant literature. [ABSTRACT FROM AUTHOR]

To design soluble artificial proteases that cleave peptide backbones of a wide range of proteins with high reactivity, artificial active sites comprising the Cu(II) complex of 1-oxa-4,7,10-triazacyclodedecane (oxacyclen) and the aldehyde group were synthesized. The aldehyde group was employed as the binding site in view of its ability to reversibly form imine bonds with ammonium groups exposed on the surfaces of proteins, and Cu(II) oxacyclen was exploited as the catalytic group for peptide hydrolysis. The artificial metalloproteases synthesized in the present study cleaved all of the protein substrates examined (albumin, γ-globulin, myoglobin, and lysozyme). In addition, the activity of the best soluble artificial protease was enhanced by up to 190-fold in terms of k/ K. When the temperature was raised to 80 °C, the activities of the artificial proteases were significantly enhanced. The activity of the artificial protease was not greatly affected by surfactants, including sodium dodecyl sulfate. The intermediacy of the imine complex formed between the artificial protease and the protein substrate was supported by an experiment using sodium cyanoborohydride. Soluble artificial metalloproteases with broad substrate selectivity, high reactivity, high thermal and chemical stabilities, and small molecular weights were thus synthesized by positioning the aldehyde group in proximity to Cu(II) oxacyclen. [ABSTRACT FROM AUTHOR]

Flavonoids have been reported to have ability to prevent cancer development. Fisetin, a naturally occurring flavonol, is commonly found in fruits, vegetables, and wine. We have previously observed that fisetin inhibits HT-29 human colon cancer cell growth. To examine the effect of fisetin on the NF-κB signaling that is known to play an important role in cell survival and apoptosis, HT-29 cells were treated with 0 or 60 µmol/L fisetin for 10 min, 30 min or 120 min. Western blot analysis revealed that fisetin increased p-IκBα protein levels at 10 and 30 min of culture, and decreased IκBα protein levels after 30 min treatment. Immunoprecipitation and in vitro kinase assay revealed that fisetin dose-dependently increased IκB kinase (IKK) activity. The subcellular fractionation and immunocytochemistry assay revealed that fisetin increased translocation of p65 (Re1A) to the nucleus. In addition, electrophoretic mobility shift assay indicated that fisetin increased NF-κB DNA binding activity in a concentration-dependent manner. These results raised the possibility that fisetin may inhibit HT-29 cell growth via its ability to upregulate NF-κB signaling. [ABSTRACT FROM AUTHOR]