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An abstract of the article "The Assemble Network: Facilitating Access to Marine Biological Infrastructures and Resources in Europe," by I. Probert, B. Kloareg, E. Brown, and M. Thorndyke is presented.

Random Amplified Polymorphic DNA (RAPD) markers were developed for genotyping in Laminaria digitata. Reproducible RAPD banding patterns were reliably obtained, using a quick micro­scale DNA extraction procedure. Intraspecific polymorphism was observed, allowing for unambiguous genotyping within sets of 3 4 sporophytes. A controlled crossing indicated that in this kelp RAPD markers are transmitted to the progeny in a Mendelian way. This approach was then applied to parentage analysis within a set of 30 anonymous sporophytes from a random cross experiment involving 4 parents. The parents of 26 offsprings were unambiguously identified, indicating that cross­fertilisation largely, if not totally, prevailed over self­fertilisation. [ABSTRACT FROM AUTHOR]

Presents observations about the isolation and characterization of microsatellite markers in the nuclear genome of brown algae. How kelps are the most abundant algae along rocky coasts; Characteristics of Laminariales; Consideration of how laminariales present a haplodiplophasic, heteromorphic cycle; Alternation of macroscopic sporophytes with microscopic gametophytes; Use of deoxyribonucleic acid (DNA) to construct two partial genomic libraries from the meristematic zone.

Marine microbial diversity offers enormous potential for discovery of compounds of crucial importance in healthcare, food security and bioindustry. However, access to it has been hampered by the difficulty of accessing and growing the organisms for study. The discovery and exploitation of marine bioproducts for research and commercial development requires state-of-the-art technologies and innovative approaches. Technologies and approaches are advancing rapidly and keeping pace is expensive and time consuming. There is a pressing need for clear guidance that will allow researchers to operate in a way that enables the optimal return on their efforts whilst being fully compliant with the current regulatory framework. One major initiative launched to achieve this, has been the advent of European Research Infrastructures. Research Infrastructures (RI) and associated centres of excellence currently build harmonized multidisciplinary workflows that support academic and private sector users. The European Marine Biological Research Infrastructure Cluster (EMBRIC) has brought together six such RIs in a European project to promote the blue bio-economy. The overarching objective is to develop coherent chains of high-quality services for access to biological, analytical and data resources providing improvements in the throughput and efficiency of workflows for discovery of novel marine products. In order to test the efficiency of this prototype pipeline for discovery, 248 rarely-grown organisms were isolated and analysed, some extracts demonstrated interesting biochemical properties and are currently undergoing further analysis. EMBRIC has established an overarching and operational structure to facilitate the integration of the multidisciplinary value chains of services to access such resources whilst enabling critical mass to focus on problem resolution. [ABSTRACT FROM AUTHOR]