1. Preparation and Characterization of a Monoclonal Antibody Specific to Histone Acetyltransferase from.
- Author
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Dvorak Montiel-Condado, Hctor Romero-Ramrez, Carmen Ramrez-Estudillo, Leopoldo Santos-Argumedo, and Rosaura Hernndez-Rivas
- Subjects
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MONOCLONAL antibodies , *PLASMODIUM falciparum , *HISTONES , *TRANSCRIPTION factors - Abstract
We have obtained a specific monoclonal antibody (MAb) against the Plasmodium falciparum histone acetyl transferase (PfGcn5), a transcriptional factor that possesses HAT activity directed to the amino terminal of histone H3. To prepare this antibody, a 968base pair (bp) DNA fragment of PfGcn5 gene corresponding to C-terminal domain was obtained by RT-PCR and cloned into prokaryotic expression vector pGEX-4T3. MAb against PfGcn5 was obtained with hybridoma technique and ELISA screening using either purified GSTPfGcn5 protein or purified GST protein alone as a control. One MAb, named Pf.r2, was able to identify the PfGcn5 protein in nuclear extract from P. falciparum and immunofluorescence assays. This MAb will be a helpful tool to perform a variety of assays to identify the other components of PfGcn5 complexes. [ABSTRACT FROM AUTHOR]
- Published
- 2005
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