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Polysaccharides have multiple pharmacological activities without toxicity or side effects. In the present study, the structure, antioxidant and lipid-lowering activities of alfalfa (Medicago sativa L.) polysaccharides (APS) were reported. Three alfalfa root polysaccharides (RAPS), RAPS-1, RAPS-2, and RAPS-3 were isolated, purified, and characterized. The structural analyses demonstrated that the homogeneous RAPS had molecular weights of 22.60, 26.95, and 38.25 kDa, respectively. Furthermore, RAPS-1 was composed of rhamnose, arabinose, xylose, glucuronic acid, glucose, and galactose with molar ratios of 10.25%, 12.53%, 6.58%, 4.56%, 15.74%, and 36.30%, RAPS-2 comprised rhamnose, arabinose, xylose, glucose, and galactose in the molar ratios of 24.07%, 14.48%, 1.24%, 15.05%, and 39.37%, while RAPS-3 was composed of rhamnose, arabinose, xylose, glucuronic acid, mannose, glucose, and galactose in the molar ratios of 31.61%, 11.78%, 1.93%, 5.49%, 3.18%, 14.56%, and 24.63%. The SEM analysis suggested that with the increase of the molecular weight of polysaccharides, its structure is more compact and the stability is stronger. In vitro , RAPS showed strong scavenging ability against DPPH and ABTS free radicals. In addition, RAPS had potent antioxidant activity and reduced the accumulation of lipids in oleic acid-induced NAFLD in HepG2 cells, with RAPS-1 being the most effective. Furthermore, RAPS-1 decreased MDA production, improved the activities of SOD and the total antioxidant capacity of cells, demonstrating that RAPS could effectively restrict lipid accumulation in HepG2 cells induced by oleic acid by restoring the antioxidant system. Western blot analysis indicated that the RAPS-1 can activate the Nrf2/HO-1 signaling pathway to relieve oxidative stress induced by oleic acid, thus playing a role in the treatment of NAFLD. [Display omitted] • Three new acidic polysaccharides were isolated from the roots of Medicago sativa L. • The polysaccharides exhibited considerable antioxidant and lipid-lowering activities. • The polysaccharides with antioxidant effect by activating Nrf2/HO-1 signaling pathway. [ABSTRACT FROM AUTHOR]

Selenium-containing polysaccharides have potential as an organic selenium dietary supplement, owing to their low toxicity, few side effects, and easy absorption attributes. In this study, we isolated two novel homogeneous selenium-containing polysaccharides from Rosa laevigata Michx fruits (Se-RLFPs). Results from primary structural analysis revealed that Se-RLFPs were α – pyranose, and were both composed of rhamnose, xylose, glucose with an average molecular weight of 24 and 16 KDa, respectively. Selenium contents in Se-RLFP-I and Se-RLFP-II were 16.49 μg/g and 21.61 μg/g, respectively. Results from analysis of antioxidant and neuroprotective activity of the polysaccharides revealed that Se-RLFPs had a radical scavenging effect. Specifically, they effectively protected SH-SY5Y cells from H 2 O 2 -induced damage by enhancing antioxidant enzyme activities (SOD), total antioxidant capacity (T-AOC) and suppressing malondialdehyde (MDA) levels. Western blots showed that the underlying mechanisms of action may be related to the Nrf2/HO-1 signaling pathway. Taken together, these results suggested that Se-RLFPs have potential as a pharmaceutical agent for treatment of neurodegenerative diseases (NDDs) or as a selenium-complementary ingredient in functional foods. [Display omitted] • Two new homogeneous selenium-containing polysaccharides (Se-RLFP-I and Se-RLFP-II) were isolated from the fruits of R. laevigata. • Se-RLFP-I and Se-RLFP-II possessed considerable antioxidant activities. • Se-RLFP-I and Se-RLFP-II exhibited significant neuroprotective activities in an SH-SY5Y cells injury model. • Se-RLFP-II could activate the Nrf2/HO-1 signaling pathway. [ABSTRACT FROM AUTHOR]

• Three new natural polysaccharides (APS1-3) isolated from Medicago Sativa L. stem. • The monosaccharide composition and glycoside bond binding sites of APS1-3 were determined. • The Se-modified products (Se-APS2-3) were synthesized. • The antioxidant and neuroprotective activities of Se-APS2-3 were higher than that of natural ones. Medicago Sativa L., a nutrient-rich plant used as feed for cattle and sheep, is widely planted globally. This study investigated the structural characteristics and activities of three kinds of novel polysaccharides (APS1, APS2 and APS3) isolated from the stems of M. sativa as well as its two selenium modified products (Se-APS2 and Se-APS3). APS1 (Mw = 13.4 KDa) and APS2 (Mw = 11.2 KDa) were composed of rhamnose, arabinose, mannose and galactose with different molar ratio, APS3 (Mw = 18.6 KDa) was composed of rhamnose, arabinose, fructose, mannose and galactose. All APS1-3 contained 1 → 3 : 1 → 6 : 1 → 4 : 1 → 2 glycosidic bonds in a ratio of 0.74:0.09:0.05:0.12, 0.34:0.20:0.36:0.10 and 0.63:0.17:0.06:0.14, respectively. The selenium content of Se-APS2 (Mw = 9.0 KDa) and Se-APS3 (Mw = 10.2 KDa) were 1.05 and 2.57 µg/mg, respectively. Their surface morphology and thermal stability were determined by scanning electron microscope (SEM) and thermal analysis (TGA), respectively. Further, the antioxidant and neuroprotective activities of the three natural polysaccharides and two Se-polysaccharides were studied. Interestingly, Se-polysaccharides not only exhibited higher antioxidant activity, but also higher neuroprotective activity compared to natural polysaccharides. [ABSTRACT FROM AUTHOR]

Highlights • Two new polysaccharides were purified from stem of P. alkekengi. • Hydrolyzed monosaccharides were identified by HPLC with a refractive index detector. • Surface characteristics of two polysaccharides are different. • Two purified polysaccharides showed some different antioxidant properties. • Purified polysaccharides possess potential Acetylcholinesterase inhibitory activity. Abstract Physalis alkekengi L. (P. alkekengi) is an important medicinal plant in China. In this study, two polysaccharides (SPAP-1 and SPAP-2) were isolated from the stem of P. alkekengi using hot water extraction method. The extract was further purified by DEAE-cellulose and Sephadex G-200 chromatography. The chemical characteristics, surface features and thermal stability of SPAP-1 and SPAP-2 were evaluated. For the first time, high-performance liquid chromatography with refractive index detection and amino column (without sample derivatization) was performed to analyze the monosaccharide composition of polysaccharides obtained from the stem of P. alkekengi. As a result, SPAP-1 (Mw = 9.1 kDa) consisted of rhamnose, arabinose, galacturonic acid, mannose, and glucose in the ratio of 1.0:1.5:1.9:29.1:1.03, while SPAP-2 (Mw = 13.5 kDa) comprised of rhamnose, xylose, arabinose, mannose and glucose in the ratio of 31.8:14.8:1.0:6.5:22.6. Additionally, the antioxidant effects of SPAP-1 and SPAP-2 were evaluated by ferric reducing power, 2,2′-Azinobis-(3-ethylbenzthiazoline-6-sulphonate acid) (ABTS) and 2.2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. The results showed that the purified polysaccharides exerted significant scavenging activities. Subsequently, acetylcholinesterase inhibitory effects in vitro of the polysaccharides were investigated, which showed that, SPAP-1 and SPAP-2 showed moderate acetylcholinesterase inhibitory activities. Thus, the polysaccharide content in P. alkekengi stem as promising source of natural products possess good antioxidant activities and may exert beneficial effects on cognitive function. [ABSTRACT FROM AUTHOR]

Abstract A phytochemical investigation on Medicago sativa L. led to the isolation of 13 compounds including three benzofuran neolignans ( 1 – 3 ), five flavonoids ( 4 – 8 ), three phenols ( 9 – 11) , and two alkaloids ( 12 and 13 ). Their structures were established through extensive spectroscopic methods (1D and 2D NMR, HRESIMS and Circular Dichroism spectrum) and comparison with literature data. Compounds 1–3 , 11 and 12 were reported for the first time from the genus Medicago and the family Leguminosae. The chemotaxonomic significance of the isolated compounds was discussed. Highlights • A phytochemical investigation on Medicago sativa L. led to the isolation of 13 compounds including one new benzofuran neolignan. • Compounds 1–3 , 11 and 12 were reported for the first time from the genus Medicago and the family of Leguminosae. • Compounds 1 – 3 , 5 , 11 and 12 could be used as potential chemotaxonomic markers. [ABSTRACT FROM AUTHOR]

The chemical profile of wood vinegar (WV) was studied by gas chromatography-mass spectrometry (GC/MS), and 48 organic compounds were identified, including phenols which accounted for 2.0%. Antioxidant compounds were isolated from WV by an activity-guided isolation method for further investigation. The dichloromethane extract from WV exhibited the highest total phenolic content and demonstrated more potent antioxidant activity than the other extracts. Then, the phenols were enriched by pH gradient extraction method from the dichloromethane extract. Seven phenols were isolated from the WV using a sequence of column chromatographic purification steps over silica gel and C18 column, followed by semi-preparative HPLC and their structures were identified by 1H NMR and 13C NMR spectra. All the compounds showed potent antioxidant activities in the 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline- 6-sulphonic acid (ABTS), and ferric-reducing antioxidant power (FRAP) assays. This study demonstrates that phenols isolated from WV have the potential to be used as antioxidant agents. [ABSTRACT FROM AUTHOR]

Xanthine oxidase (XO) has emerged as an important target for the treatment of hyperuricemia and gout. In this study, to obtain novel nonpurine XO inhibitors, a series of 1-alkyl-5/6-(5-oxo-4,5-dihydro-1,2,4-oxadiazol-3-yl)-1 H -indole-3-carbonitriles (1a-1u , 2c , 2e , 2h and 2n) were designed using a bioisosteric replacement strategy and were synthesized through a five-step procedure with good yields. Thereafter, the in vitro XO inhibitory potencies of these compounds were evaluated by spectrophotometry, showing inhibitory profiles in the micromolar/submicromolar range. Particularly, compound 1h emerged as the strongest XO inhibitor, with an IC 50 value of 0.36 μM, which was approximately 21-fold more potent than the positive control allopurinol. Additionally, the structure-activity relationships revealed that the 5-oxo-4,5-dihydro-1,2,4-oxadiazole moiety linked at the 5-position of the indole scaffold was more preferable than the 6-position for the XO inhibitory potency. Enzyme kinetic studies indicated that compound 1h acted as a mixed-type XO inhibitor. Moreover, molecular modeling studies were performed on compound 1h to gain insights into its binding modes with XO. The results showed that the 5-oxo-4,5-dihydro-1,2,4-oxadiazole moiety could interact with Arg880 and Thr1010 in the innermost part of the active pocket through hydrogen bonds, while the cyano group could form hydrogen bonds with Asn768 and Lys771 in the subpocket. Furthermore, the in vivo hypouricemic effect of compound 1h was further investigated in a hyperuricemia rat model induced by potassium oxonate. The results suggested that compound 1h could effectively reduce serum uric acid levels at an oral dose of 10 mg/kg. Therefore, compound 1h could be a promising lead compound for the treatment of hyperuricemia and gout. Image 1 • 1-Alkyl-5/6-(5-oxo-4,5-dihydro-1,2,4-oxadiazol-3-yl)-1 H -indole-3-carbonitriles were synthesized. • The structure-activity relationships of the synthesized compounds were summarized. • Compound 1h exhibited a promising XO inhibitory potency with an IC 50 value of 0.36 μM. • Enzyme kinetics, molecular modeling and in vivo hypouricemic effect studies were performed. [ABSTRACT FROM AUTHOR]

Depression is a common mental health disease, with the characteristics of significant and persistent low mood. Traditionally, Ziziphus jujuba Mill. was used to treat depression. However, its chemical pharmacologic profiles remain to be elucidated. The present study was aimed to explore the potential targets and mechanisms of Z. jujuba in the treatment of depression using network pharmacology and in vitro neuroprotective experiment. In this study, 11 compounds of Z. jujuba were obtained by multiple column chromatography. Based on the network pharmacology, the C-T network was established by the results of molecular docking. The information on target proteins was enriched via DAVID database, followed by the analysis of GO and KEGG. The results indicated that 10 compounds, 5 target proteins and 3 signaling pathways played important roles in treating depression. Based on the predicted result of network pharmacology, compounds 1, 9 and 10 were identified with significant neuroprotective effects by in vitro cell experiments with human neuroblastoma cell line SH-SY5Y. This study was the first to investigate the mechanisms of Z. jujuba action in the treatment of depression from the system level, and could provide the basis of the mechanism with action of compounds for the in-depth utilization of Z. jujuba. [ABSTRACT FROM AUTHOR]

Rosa laevigata Michx., a member of the Rosaceae family, is a famous traditional Chinese medicine and edible food in Asian countries. The major active ingredient is triterpenoid, which has anti‐Alzheimer's Disease (AD), antioxidant and neuroprotection properties. However, the properties of "multi‐component structure" and "multi‐directional target" make it impossible to explain the molecular mechanism by conventional ways. The present study aimed to predict the multi‐target mechanisms of triterpenoids in R. laevigata against AD using high performance liquid chromatography equipped with quadrupole time‐of‐flight mass spectrometer (HPLC‐Q‐TOF MS) approach, network pharmacology analysis, dynamics simulation (MDs) and validation of the predicted results with straight‐target tests as well as neuroprotection model. Firstly, bioassay‐guided fractionation of R. laevigata integrated with LC‐MS/MS analysis of the most prospective enriched fractions in terms of anti‐AD efficacy was commenced. Subsequently, multi‐level network model of "multi components‐multi‐targets‐multiple pathways" was established based on the identification of 69 triterpenoids. Complementarily, 64 AD‐related targets were identified through various databases. Network structure analysis illuminated that the intricate pharmacological mechanisms improving cognitive dysfunction of AD was superiorly combined with the crucial targets of APP, APOE, VEGFA, IL1B, MAPT, PSEN1 and BACE and further regulation of principal pathways as HIF‐1 and Calcium signaling pathways. In addition, AChE, BChE and BACE inhibitory activities in vitro showed that nine compounds exhibited potential inhibitory activities. The neuroprotective effects also had a similar effect on H2O2‐induced SH‐SY5Y cell injury. The results show that R. laevigata and its bioactive triterpenoids can pave the way for potential application as inhibitors for treatment of AD. [ABSTRACT FROM AUTHOR]

A set of conditions for the Strecker reaction of formaldehyde, amines, and TMSCN that affords three‐component coupling adducts as the major products is reported. HCOOH and TBAF are used as cocatalysts and the TMSCN functions as both cyanide source and protecting reagent for amines. As a result, the side reactions were suppressed efficiently and a broad range of aromatic and aliphatic amines were mono‐cyanomethylated in 52–99% yield. Furthermore, this protocol was successfully applied toward the synthesis of meta‐diamide insecticides. [ABSTRACT FROM AUTHOR]

Summary: Considering the medicinal and edible properties of Physalis pubescens L. fruit, the plant has a long history of cultivation in China. In the current study, a novel polysaccharide (PPL‐1) was successfully obtained from P. pubescens fruits using multi‐column techniques. The chemical characterisation of the polysaccharide was achieved by acid hydrolysis, determination of weight average molecular mass, thermogravimetric and Smith degradation analyses, in addition to ultraviolet, Fourier transform infrared spectroscopy, one‐ and two‐dimensional nuclear magnetic resonance spectroscopy. According to the structural analyses, PPL‐1 consisted of rhamnose, arabinose, fructose, mannose and glucose with a relative molar ratio of 0.39:0.12:0.02:0.03:0.44. The average molecular weight of PPL‐1 was 7.3 kDa and it was mainly composed of (1 → 3) and (1 → 6) linkages. PPL‐1 exhibited not only scavenging effects on 2,2‐diphenyl‐1‐picrylhydrazyl and 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) free radicals but also hydrogen peroxide‐induced oxidative stress in SH‐SY5Y cells effectively by decreasing malondialdehyde content and increasing total antioxidant capacity, superoxide dismutase and glutathione peroxidase activity levels. [ABSTRACT FROM AUTHOR]

This study explored the structures of three polysaccharides from Bupleurum chinense DC. (BCPRs), and evaluated their antioxidant and anti-aging properties. The HPGPC and ion chromatography analyses revealed that the molecular weights of the BCPRs ranged from 12.05 to 21.20 kDa, and were primarily composed of rhamnose, arabinose, xylose, galactose, glucose and galacturonic acid. Methylation and NMR studies identified 10 PMAAs, establishing the various backbones of BCPRs 1–3. BCPR-3 demonstrated potent antioxidant activities, including DPPH, ABTS, hydroxy, and superoxide radicals scavenging in vitro. At concentrations between 125 and 500 μg/mL, BCPR-3 increased T-AOC, SOD and GSH-Px activities, while decreasing MDA levels in H 2 O 2 -induced SH-SY5Y cells. In addition, RNA-seq results indicated that BCPR-3 considerably downregulated the expression of 49 genes and upregulated five genes compared with the control group. KEGG analysis suggested that these differentially expressed genes (DEGs) were predominantly involved in the TNF and PI3K/Akt signaling pathways. Furthermore, in vivo experiment with Drosophila melanogaster showed that BCPR-3 could extend the average lifespan of flies. In conclusion, polysaccharides from B. chinense exhibited potential antioxidant and anti-aging activities, which could be developed as new ingredients to combat oxidative stress damage and slow the aging process. [Display omitted] • Three acid polysaccharides were isolated from the roots of Bupleurum chinense DC. • BCPR-1, BCPR-2 and BCPR-3 possessed antioxidant activities. • BCPR-3 had a protective effect on SH-SY5Y cell injury induced by H 2 O 2. • BCPR-3 exerts antioxidant effect by regulating TNF and PI3K/Akt signaling pathways. • BCPR-3 could relatively prolong the life span of Drosophila melanogaster. [ABSTRACT FROM AUTHOR]

This study explored the structures of three polysaccharides from Bupleurum chinense DC. (BCPRs), and evaluated their antioxidant and anti-aging properties. The HPGPC and ion chromatography analyses revealed that the molecular weights of the BCPRs ranged from 12.05 to 21.20 kDa, and were primarily composed of rhamnose, arabinose, xylose, galactose, glucose and galacturonic acid. Methylation and NMR studies identified 10 PMAAs, establishing the various backbones of BCPRs 1–3. BCPR-3 demonstrated potent antioxidant activities, including DPPH, ABTS, hydroxy, and superoxide radicals scavenging in vitro. At concentrations between 125 and 500 μg/mL, BCPR-3 increased T-AOC, SOD and GSH-Px activities, while decreasing MDA levels in H 2 O 2 -induced SH-SY5Y cells. In addition, RNA-seq results indicated that BCPR-3 considerably downregulated the expression of 49 genes and upregulated five genes compared with the control group. KEGG analysis suggested that these differentially expressed genes (DEGs) were predominantly involved in the TNF and PI3K/Akt signaling pathways. Furthermore, in vivo experiment with Drosophila melanogaster showed that BCPR-3 could extend the average lifespan of flies. In conclusion, polysaccharides from B. chinense exhibited potential antioxidant and anti-aging activities, which could be developed as new ingredients to combat oxidative stress damage and slow the aging process. [Display omitted] • Three acid polysaccharides were isolated from the roots of Bupleurum chinense DC. • BCPR-1, BCPR-2 and BCPR-3 possessed antioxidant activities. • BCPR-3 had a protective effect on SH-SY5Y cell injury induced by H 2 O 2. • BCPR-3 exerts antioxidant effect by regulating TNF and PI3K/Akt signaling pathways. • BCPR-3 could relatively prolong the life span of Drosophila melanogaster. [ABSTRACT FROM AUTHOR]

Hot water extraction and chromatographic purification methods were used to extract and purify two polysaccharides (RAPS-1 and RAPS-2) from the roots of alfalfa. Subsequently, RAPS-2 was modified using the HNO 3 /Na 2 SeO 3 method to obtain Se-RAPS-2. The structural features, antioxidant and in vitro anti-tumor activities of the three polysaccharides were evaluated. The structural analysis revealed that RAPS-1 (Mw = 10.0 kDa) was composed of rhamnose, xylose, arabinose, galacturonic acid, mannose and glucose, whereas RAPS-2 (Mw = 15.8 kDa) consisted of rhamnose, xylose, galacturonic acid, mannose, glucose and galactose. RAPS-1 contained 1 → 2, 1 → 4, 1 → 3, and 1 → 6 or 1 → glycosidic bonds; however, while RAPS-2 lacked 1 → 4 glycosidic linkages. The molecular weight of Se-RAPS-2 was 11.0 kDa less than that of RAPS-2. The results of activities demonstrated that Se-RAPS-2 displayed superior antioxidant activity and inhibitory effect in HepG2 cells than RAPS-1 and RAPS-2. • Two new polysaccharides were purified from roots of alfalfa. • The polysaccharide of alfalfa roots was selenylated for the first time. • Selenylated polysaccharide possesses superior antioxidant and anti-tumor activities. [ABSTRACT FROM AUTHOR]

Eleven lignans (1 – 11), one oxylipin vanillyl acetal (12), one monoterpene (13) and one fatty acid (14) were isolated from the roots of Bupleurum scorzonerifolium Willd. The structures of these compounds were elucidated on the basis of NMR spectroscopic methods, as well as by comparison with published data. Among all the isolated compounds, dextrobursehernin (1), 2 R ,3 R -2 β -(4''-hydroxy-3''- methoxybenzyl)-3 α -(4′-hydroxy-3′-methoxybenzyl)- γ -butyrolactone-2- O -(β -D-glucopyranoside) (5), 7 S ,8 S -4,7,9,9′-tetrahydroxy-3,2′-dimethoxy-4′-allyl-8,4′-oxyneolignan (8), 7 R ,8 S -4,7,9,9′-tetrahydroxy-3,2′-dimethoxy-4′-allyl-8,4′-oxyneolignan (9), syringaresinol 4′- O - β -D-glucopyranoside (11), solacetal C (12), bornyl 6- O - β -D- apiofuranosyl (1 → 6)- β -D-glucopyranoside (13), and methyl-9,12,13- trihydroxyoctadeca-10,15-dienoate (14) were reported from the genus Bupleurum and the family Umbelliferae for the first time. In addition, compound-family network analysis was carried out, and the chemotaxonomic significance of the isolated compounds was also summarized. • Fourteen compounds were isolated from the roots of Bupleurum scorzonerifolium WilId. • Compounds 1–2, 5–6, 8–14 were firstly reported from B. scorzonerifolium. • Some compounds were first described in Bupleurum genus and Umbelliferae family. • The compound-family network analysis was constructed. • The chemotaxonomic significance of the isolated compounds was discussed. [ABSTRACT FROM AUTHOR]

Front Cover Picture: Strecker Reactions of Formaldehyde with TMSCN, Catalyzed by TBAF and Formic Acid: N-Monocyanomethylation of Primary Amines (Adv. Strecker reaction, formaldehyde, TMSCN, N-monocyanomethylation, meta-diamide insecticides Keywords: Strecker reaction; formaldehyde; TMSCN; N-monocyanomethylation; meta-diamide insecticides EN Strecker reaction formaldehyde TMSCN N-monocyanomethylation meta-diamide insecticides 1 1 1 01/20/23 20230110 NES 230110 The front cover picture illustrates the Strecker reaction of formaldehyde, primary amines and TMSCN. [Extracted from the article]

Three new pentacyclic triterpenoid saponins (1 – 3), together with medicagenic acid (4) were isolated and purified from 70% EtOH extract of Medicago sativa L. by different column chromatographic and semi-preparative HPLC. Their structures were established by direct interpretation of their spectral data, mainly HR-ESI-MS, 1D-NMR, 2D-NMR, and chemical methods, as well as comparison with literature data. Additionally, all isolates were evaluated for their neuroprotective activities against H 2 O 2 -induced damage in human neuroblastoma SHSY5Y cells. As a results, compounds 1 and 2 (67.14% and 73.05%) exhibited potent neuroprotective activities. These findings provide new insights into developing better treatment of neurodegenerative diseases for M. sativa in the future. [ABSTRACT FROM AUTHOR]

A phytochemical investigation of the roots of Bupleurum chinese DC. led to the isolation of eighteen compounds, including three chromones (1 – 3), seven flavonoids (4 – 10), one dihydrochalcone (11), two phenylpropanoid glycosides (12–13), one lignan (14), three sterols (15 – 17) and one stilbenoid (18). Their structures were elucidated on the basis of NMR spectroscopic analysis and comparison with literature data. Compounds 6–7 , 11–14 and 18 were firstly reported from the genus Bupleurum and the family Apiaceae. Moreover, the chemotaxonomic value of the isolates was also discussed. • The phytochemical investigation of Bupleurum chinese DC. resulted in 18 compounds. • Compounds 6–7, 11–14, 18 were reported from the genus Bupleurum and family Apiaceae for the first time. • Compounds 6–7, 11–14, 18 could serve as potential chemotaxonomic markers to identify B. chinense. [ABSTRACT FROM AUTHOR]