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The article proposes a multi-objective optimization method called Generalized Differential Evolution (GDE3) for a Variable Number of Dimensions (VND). The well-known generalized differential evolution is adapted to handle problems where the number of decision space variables is not a priori known. The performance of the method is assessed on a set of benchmark problems based on standard multi-objective test cases modified to depend on the number of decision space variables. Experimental results include a study of the setting of controlling parameters and a comparison with the state-of-the-art Variable-Length GDE3 (VLGDE3) algorithm. Finally, the novel approach is compared to the standard approach in a linear antenna array design problem. [ABSTRACT FROM AUTHOR]

In this article we analyze a localization problem based on evolutionary optimization algorithms and an analytical electromagnetic-field-to-line coupling model. A computationally efficient, analytical model for calculating time-domain lightning-induced voltages serves as the forward solver in the optimization process. The model takes into account electric permittivity and conductivity of a lossy ground. The inverse problem is solved with the aid of a single-objective global optimization algorithm. It is demonstrated that the covariant matrix adaptation - evolution strategy algorithm shows the best performance among considered state-of-the-art algorithms. The influence of other problem parameters, e.g., the size of the search space, the fluctuation of the return-stroke pulsewidth and amplitude, and the presence of noise, on the localization error is discussed. While the growing size of the search domain has a significant impact on the convergence properties of the optimization process, this is not the case for the signal distortion, whose influence can be virtually neglected. [ABSTRACT FROM AUTHOR]

A single‐ and multi‐objective optimization package is presented and described in detail. It contains an ensemble of local and global optimization routines. Procedures controlling variable number of dimensions are implemented as well, which is a rare feature among optimization oriented packages. The package is provided as a MATLAB toolbox. It excels in versatility and extensibility, which is demonstrated on a series of examples covering classical electromagnetism and antenna design. It is taken for granted that defining parameters of the optimization method can be set prior to the simulation run. However, its effective performance can be changed during the optimization run thanks to the full control feature. Moreover, it opens new possibilities in merging various algorithms into hybrids, performing complex dynamic programming tasks, or exploiting third party software. These advantages render the package as a perfect tool to deal with nowadays challenging engineering tasks. [ABSTRACT FROM AUTHOR]

The decoupling of modern printed circuit boards introduces a very complex task. Powerful stochastic optimizers are usually used to determine values and positions of decoupling capacitors on the board. The number of capacitors used has to be determined a priori by the user which brings problems with convergence of the optimization process or can lead to a waste of resources when the noises are to be attenuated to a certain level. In this paper, an approach based on the combination of time-domain contour integral method and optimization with variable number of dimensions is introduced. The optimizer works with models having variable dimensions and searches for the optimal one. The approach is tested on two example power circuit boards with various noise attenuation limits and constraints on capacitor positions and values. [ABSTRACT FROM AUTHOR]

Designing a composite, possibly strengthened by a dispersion of (fine) oxides, is a favorable way to improve the mechanical characteristics of Cu while maintaining its advantageous electric conductivity. The aim of this study was to perform mechanical alloying of a Cu powder with a powder of Al2O3 oxide, seal the powder mixture into evacuated Cu tubular containers, i.e., cans, and apply gradual direct consolidation via rotary swaging at elevated temperatures, as well as at room temperature (final passes) to find the most convenient way to produce the designed Al2O3 particle-strengthened Cu composite. The composites swaged with the total swaging degree of 1.83 to consolidated rods with a diameter of 10 mm were subjected to measurements of electroconductivity, investigations of mechanical behavior via compression testing, and detailed microstructure observations. The results revealed that the applied swaging degree was sufficient to fully consolidate the canned powders, even at moderate and ambient temperatures. In other words, the final structures, featuring ultra-fine grains, did not exhibit voids or remnants of unconsolidated powder particles. The swaged composites featured favorable plasticity regardless of the selected processing route. The flow stress curves exhibited the establishment of steady states with increasing strain, regardless of the applied strain rate. The electroconductivity of the composite swaged at elevated temperatures, featuring homogeneous distribution of strengthening oxide particles and the average grain size of 1.8 µm2, reaching 80% IACS (International Annealed Copper Standard). [ABSTRACT FROM AUTHOR]

Schistosomiasis, caused by the parasitic flatworm Schistosoma mansoni and related species, is a tropical disease that affects over 200 million people worldwide. A new approach for targeting eukaryotic parasites is to tackle their dynamic epigenetic machinery that is necessary for the extensive phenotypic changes during the life cycle of the parasite. Recently, we identified S. mansoni histone deacetylase 8 (smHDAC8) as a potential target for antiparasitic therapy. Here, we present results on the investigations of a focused set of HDAC ( h istone d e ac etylase) inhibitors on smHDAC8. Besides several active hydroxamates, we identified a thiol-based inhibitor that inhibited smHDAC8 activity in the micromolar range with unexpected selectivity over the human isotype, which has not been observed so far. The crystal structure of smHDAC8 complexed with the thiol derivative revealed that the inhibitor is accommodated in the catalytic pocket, where it interacts with both the catalytic zinc ion and the essential catalytic tyrosine (Y341) residue via its mercaptoacetamide warhead. To our knowledge, this is the first complex crystal structure of any HDAC inhibited by a mercaptoacetamide inhibitor, and therefore, this finding offers a rationale for further improvement. Finally, an ester prodrug of the thiol HDAC inhibitor exhibited antiparasitic activity on cultured schistosomes in a dose-dependent manner. [ABSTRACT FROM AUTHOR]

The use of DNA-based analyses in molecular plant nematology research has dramatically increased over recent decades. Therefore, the development and adaptation of simple, robust, and cost-effective DNA purification procedures are required to address these contemporary challenges. The solid-phase-based approach developed by Flinders Technology Associates (FTA) has been shown to be a powerful technology for the preparation of DNA from different biological materials, including blood, saliva, plant tissues, and various human and plant microbial pathogens. In this work, we demonstrate, for the first time, that this FTA-based technology is a valuable, low-cost, and time-saving approach for the sampling, long-term archiving, and molecular analysis of plant-parasitic nematodes. Despite the complex structure and anatomical organization of the multicellular bodies of nematodes, we report the successful and reliable DNA-based analysis of nematode high-copy and low-copy genes using the FTA technology. This was achieved by applying nematodes to the FTA cards either in the form of a suspension of individuals, as intact or pestle-crushed nematodes, or by the direct mechanical printing of nematode-infested plant tissues. We further demonstrate that the FTA method is also suitable for the so-called "one-nematode-assay", in which the target DNA is typically analyzed from a single individual nematode. More surprisingly, a time-course experiment showed that nematode DNA can be detected specifically in the FTA-captured samples many years after initial sampling occurs. Collectively, our data clearly demonstrate the applicability and the robustness of this FTA-based approach for molecular research and diagnostics concerning phytonematodes; this research includes economically important species such as the stem nematode (Ditylenchus dipsaci), the sugar beet nematode (Heterodera schachtii), and the Northern root-knot nematode (Meloidogyne hapla). [ABSTRACT FROM AUTHOR]

The treatment of schistosomiasis, a disease caused by blood flukes parasites of the Schistosoma genus, depends on the intensive use of a single drug, praziquantel, which increases the likelihood of the development of drug-resistant parasite strains and renders the search for new drugs a strategic priority. Currently, inhibitors of human epigenetic enzymes are actively investigated as novel anti-cancer drugs and have the potential to be used as new anti-parasitic agents. Here, we report that Schistosoma mansoni histone deacetylase 8 (smHDAC8), the most expressed class I HDAC isotype in this organism, is a functional acetyl-L-lysine deacetylase that plays an important role in parasite infectivity. The crystal structure of smHDAC8 shows that this enzyme adopts a canonical α/β HDAC fold, with specific solvent exposed loops corresponding to insertions in the schistosome HDAC8 sequence. Importantly, structures of smHDAC8 in complex with generic HDAC inhibitors revealed specific structural changes in the smHDAC8 active site that cannot be accommodated by human HDACs. Using a structure-based approach, we identified several small-molecule inhibitors that build on these specificities. These molecules exhibit an inhibitory effect on smHDAC8 but show reduced affinity for human HDACs. Crucially, we show that a newly identified smHDAC8 inhibitor has the capacity to induce apoptosis and mortality in schistosomes. Taken together, our biological and structural findings define the framework for the rational design of small-molecule inhibitors specifically interfering with schistosome epigenetic mechanisms, and further support an anti-parasitic epigenome targeting strategy to treat neglected diseases caused by eukaryotic pathogens. [ABSTRACT FROM AUTHOR]

Recently, it has been shown that the stem nematode, Ditylenchus dipsaci (Nematoda: Anguinidae), is genetically more related to the gall-forming nematodes from genera Anguina, Heteroanguina, and Mesoanguina than to other members of the genus Ditylenchus. This finding was provided by molecular data written in the evolutionary variable, non-coding internal transcribed spacers (ITS1 and ITS2) of the ribosomal DNA (rDNA). In the current paper, we analyze the nucleotide sequences and predict the secondary structures of two expansion segments (D2, D3) of the 28S ribosomal RNA (rRNA)-coding gene for the plant-parasitic nematodes from the genus Ditylenchus and their related anguinids. In general, the expansion segment D2 appeared to be more variable than the segment D3 illustrating their different evolutionary constraints. Comparative analysis of the aligned sequences and predicted secondary structures revealed similar trend showing the tight relationships between the stem nematodes (D. dipsaci, D. gigas, D. weischeri) and gall-forming nematodes from the subfamily Anguininae. Phylogeny reconstructions disjoined the family Anguinidae into two monophyletic clusters (Clade 1 and 2). Clade 1 constitutes the stem nematodes (D. dipsaci, D. gigas, etc) and gall-forming nematodes from the genera Anguina, Heteroanguina, Subanguina and Mesoanguina, while clade 2 includes other Ditylenchus species like D. destructor and D. halictus. Collectively, deciphering the exact phylogenetic relationships within the family Anguinidae (Nematoda: Tylenchida) with respect to our results should provide a framework for a taxonomic revision in order to reflect biological history of these nematodes. In addition, we provide novel molecular data, which may be exploited in diagnostic tools for phytosanitary control of these economically important plant parasites. [ABSTRACT FROM AUTHOR]

Abstract: Mouse polyomavirus (MPyV) VP1-pseudocapsids carrying enhanced green fluorescent protein (EGFP-VLPs) were used for intranasal immunization of mice. EGFP-VLPs induced strong anti-VP1 but not anti-EGFP antibody production. In vitro restimulation with antigen-pulsed bone marrow-derived dendritic cells (BMDCs) induced remarkable T-cell proliferative response specific for both VP1 and EGFP antigen and IL-2 and IFN-γ production. Surprisingly, no specific cytotoxic activities against VP1 and EGFP proteins were detected. After intranasal administration of EGFP-VLPs, as well as after polyomavirus infection, a moderate reduction of CD4+CD25+Foxp3+ T cells was observed in spleens but not in lymph nodes and peripheral blood, suggesting that both MPyV virions and pseudocapsids are able to induce changes in distribution of regulatory T cells. Treatment of EGFP-VLPs pulsed BMDCs with inhibitors of endosomal acidification proved that presentation of peptides on MHCgp class II is dependent on acidic endosomal environment. Substantial decrease of CD4-specific T-cell proliferation in the presence of proteasome inhibitor suggests that MHCgp class II might load VPL-derived peptides processed by proteasomes. Thus, polyomavirus derived VLPs appear to be promising delivery and adjuvant vehicles for therapeutic proteins. [Copyright &y& Elsevier]

Haloalkane dehalogenases (HLDs) are a family of α/β‐hydrolase fold enzymes that employ SN2 nucleophilic substitution to cleave the carbon–halogen bond in diverse chemical structures, the biological role of which is still poorly understood. Atomic‐level knowledge of both the inner organization and supramolecular complexation of HLDs is thus crucial to understand their catalytic and noncatalytic functions. Here, crystallographic structures of the (S)‐enantioselective haloalkane dehalogenase DmmarA from the waterborne pathogenic microbe Mycobacterium marinum were determined at 1.6 and 1.85 Å resolution. The structures show a canonical αβα‐sandwich HLD fold with several unusual structural features. Mechanistically, the atypical composition of the proton‐relay catalytic triad (aspartate–histidine–aspartate) and uncommon active‐site pocket reveal the molecular specificities of a catalytic apparatus that exhibits a rare (S)‐enantiopreference. Additionally, the structures reveal a previously unobserved mode of symmetric homodimerization, which is predominantly mediated through unusual L5‐to‐L5 loop interactions. This homodimeric association in solution is confirmed experimentally by data obtained from small‐angle X‐ray scattering. Utilizing the newly determined structures of DmmarA, molecular modelling techniques were employed to elucidate the underlying mechanism behind its uncommon enantioselectivity. The (S)‐preference can be attributed to the presence of a distinct binding pocket and variance in the activation barrier for nucleophilic substitution. [ABSTRACT FROM AUTHOR]

Environmentally friendly industrial and biotech processes greatly benefit from enzyme‐based technologies. Their use is often possible only when the enzyme‐catalytic mechanism is thoroughly known. Thus, atomic‐level knowledge of a Michaelis enzyme‐substrate complex, revealing molecular details of substrate recognition and catalytic chemistry, is crucial for understanding and then rationally extending or improving enzyme‐catalyzed reactions. However, many known enzymes sample huge protein conformational space, often preventing complete structural characterization by X‐ray crystallography. Moreover, using a cognate substrate is problematic since its conversion into a reaction product in the presence of the enzyme will prevent the capture of the enzyme‐substrate conformation in an activated state. Here, we outlined how to deal with such obstacles, focusing on the recent discovery of a Renilla‐type bioluminescence reaction mechanism facilitated by a combination of engineered ancestral enzyme and the availability of a non‐oxidizable luciferin analogue. The automated ancestral sequence reconstructions using FireProtASR provided a thermostable enzyme suited for structural studies, and a stable luciferin analogue azacoelenterazine provided a structurally cognate chemical incapable of catalyzed oxidation. We suggest that an analogous strategy can be applied to various enzymes with unknown catalytic mechanisms and poor crystallizability. [ABSTRACT FROM AUTHOR]

An abstract of the article "Essential C-Terminal Region of the Baculovirus Minor Capsid Protein VP80 Binds DNA," by Martin Marek and colleagues is presented.

Recently, we showed that the Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) VP80 protein is essential for the formation of both virion types, budded virus (BV) and occlusion-derived virus (ODV). Deletion of the vp80 gene did not affect assembly of nucleocapsids. However, these nucleocapsids were not able to migrate from the virogenic stroma to the nuclear periphery. In the current paper, we constructed a baculovirus recombinant with enhanced-green fluorescent protein (EGFP)-tagged VP80, allowing visualization of the VP80 distribution pattern during infection. In baculovirus-infected cells, the EGFP-VP80 protein is entirely localized in nuclei, adjacent to the virus-triggered F-actin scaffold that forms a highly organized three-dimensional network connecting the virogenic stroma physically with the nuclear envelope. Interaction between VP80 and host actin was confirmed by coimmunoprecipitation. We further showed that VP80 is associated with the nucleocapsid fraction of both BVs and ODVs, typically at one end of the nucleocapsids. In addition, the presence of sequence motifs with homology to invertebrate paramyosin proteins strongly supports a role for VP80 in the polar transport of nucleocapsids to the periphery of the nucleus on their way to the plasma membrane to form BVs and for assembly in the nuclear periphery to form ODVs for embedding in viral occlusion bodies. [ABSTRACT FROM AUTHOR]

Histone deacetylase 6 (HDAC6) catalyzes the removal of an acetyl group from lysine residues of several non-histone proteins. Here we report the preparation of thiazole-, oxazole-, and oxadiazole-containing biarylhydroxamic acids by a short synthetic procedure. We identified them as selective HDAC6 inhibitors by investigating the inhibition of recombinant HDAC enzymes and the protein acetylation in cells by Western blotting (tubulin vs histone acetylation). The most active compounds exhibited nanomolar potency and high selectivity for HDAC6. For example, an oxazole hydroxamate inhibits HDAC6 with an IC50 of 59 nM and has a selectivity index of >200 against HDAC1 and HDAC8. This is the first report showing that the nature of a heterocycle directly connected to a zinc binding group (ZBG) can be used to modulate subtype selectivity and potency for HDAC6 inhibitors to such an extent. We rationalize the high potency and selectivity of the oxazoles by molecular modeling and docking. [ABSTRACT FROM AUTHOR]

The article deals with the analysis of chromium layer grinding on a steel substrate, where this issue was addressed with regard to the requirements of the manufacturing sector, specifically in the aerospace industry. The experimental samples were chromium-plated and ground under different cutting conditions by the grooving method of grinding. Two types of grinding wheels for grinding were used, grinding wheel based on SG (solgel) a grinding wheel based on SiC. The resulting microstructure and microhardness in the machined layer were evaluated with using of confocal laser microscopy, inverted materials microscopy, and hardness testing. Based on the results, recommendations were made regarding a suitable approach to grinding the chromium coating. We used a confocal laser microscope and hardness tester for the evaluation of presented values. It was found that, on the base of analyses values, with both grinding wheel and using cutting conditions used, good results have been achieved. This could be stated, because the analysis of the samples microstructure after grinding for the given cutting conditions showed that it is possible that a small influence is completely acceptable from the point of the final product view and there are no major negative phenomena. Measurements of surface microhardness after grinding showed similar results for all samples. The SiC-based grinding wheel showed slightly better results, but both grinding wheels can be used without problems for the presented cutting conditions, and the presented cutting conditions with both grinding wheels can be recommended for the grinding of the given material. [ABSTRACT FROM AUTHOR]

A series of hydroxamic acids linked by different lengths to a chiral imidazoketopiperazine scaffold were synthesized. The compounds with linker lengths of 6 and 7 carbon atoms were the most potent in histone deacetylase (HDAC) inhibition, and were specific submicromolar inhibitors of the HDAC1, HDAC6 and HDAC8 isoforms. A docking model for the binding mode predicts binding of the hydroxamic acid to the active site zinc cation and additional interactions between the imidazo-ketopiperazine and the enzyme rim. The compounds were micromolar inhibitors of the MV4-11, THP-1 and U937 cancer cell lines. Increased levels of histone H3 and tubulin acetylation support a cellular mechanism of action through HDAC inhibition. This article is part of a discussion meeting issue 'Frontiers in epigenetic chemical biology'. [ABSTRACT FROM AUTHOR]

A promising means in the search of new small molecules for the treatment of schistosomiasis (amongst other parasitic ailments) is by targeting the parasitic epigenome. In the present study, a docking based virtual screening procedure using the crystal structure of histone deacetylase 8 from Schistosoma mansoni (smHDAC8) was designed. From the developed screening protocol, we were able to identify eight novel N-(2,5-dioxopyrrolidin-3-yl)-n-alkylhydroxamate derivatives as smHDAC8 inhibitors with IC50 values ranging from 4.4-20.3 µM against smHDAC8. These newly identified inhibitors were further tested against human histone deacetylases (hsHDAC1, 6 and 8), and were found also to be exerting interesting activity against them. In silico prediction of the docking pose of the compounds was confirmed by the resolved crystal structure of one of the identified hits. This confirmed these compounds were able to chelate the catalytic zinc ion in a bidentate fashion, whilst showing an inverted binding mode of the hydroxamate group when compared to the reported smHDAC8/hydroxamates crystal structures. Therefore, they can be considered as new potential scaffold for the development of new smHDAC8 inhibitors by further investigation of their structure-activity relationship. [ABSTRACT FROM AUTHOR]

In this work, we report the multicomponent synthesis of a focused histone deacetylase (HDAC) inhibitor library with peptoid-based cap groups and different zinc-binding groups. All synthesized compounds were tested in a cellular HDAC inhibition assay and an MTT assay for cytotoxicity. On the basis of their noteworthy activity in the cellular HDAC assays, four compounds were further screened for their inhibitory activity against recombinant HDAC1-3, HDAC6, and HDAC8. All four compounds showed potent inhibition of HDAC1-3 as well as significant inhibition of HDAC6 with IC50 values in the submicromolar concentration range. Compound 4j, the most potent HDAC inhibitor in the cellular HDAC assay, revealed remarkable chemosensitizing properties and enhanced the cisplatin sensitivity of the cisplatin-resistant head-neck cancer cell line Cal27CisR by almost 7-fold. Furthermore, 4j almost completely reversed the cisplatin resistance in Cal27CisR. This effect is related to a synergistic induction of apoptosis as seen in the combination of 4j with cisplatin. [ABSTRACT FROM AUTHOR]

The synthesis and biological evaluation of potent hydroxamate-based dual HDAC1/6 inhibitors with modest HDAC6 preference and a novel alkoxyurea connecting unit linker region are described. The biological studies included the evaluation of antiproliferative effects and HDAC inhibitory activity in the human ovarian cancer cell line A2780, the human squamous carcinoma cell line Cal27, and their cisplatin resistant sublines A2780CisR and Cal27CisR. The three most potent compounds 1g-i showed IC50 values in the low μM and sub-μM range. 1g-i revealed low nM IC50 values for HDAC6 with up to 15-fold preference over HDAC1, >3500-fold selectivity over HDAC4, and >100-fold selectivity over HDAC8. Furthermore, their ability to enhance cisplatin sensitivity was analyzed in Cal27 and Cal27CisR cells. Notably, a 48 h preincubation of 1g-i significantly enhanced the antiproliferative effects of cisplatin in Cal27 and Cal27CisR. 1g-i interacted synergistically with cisplatin. These effects were more pronounced for the cisplatin resistant subline Cal27CisR. [ABSTRACT FROM AUTHOR]

The twist channel angular pressing (TCAP) technology was recently developed in accordance to the contemporary trend of increasing the efficiency of severe plastic deformation technologies. This study was aimed to analyse structural changes, particularly grain sizes and orientations and textures, in commercial purity aluminium after a single pass TCAP and to put the structure in connection with the strain path. For comparison, another sample processed by two passes equal channel angular pressing (ECAP), Bc route, was analysed. Three independent shear planes affecting the material during a single pass TCAP caused the grains to refine down to the average size of 5.8 µm. The sample after a TCAP pass also exhibited highly developed subgrains. The textures of the samples exhibited differences in preferential orientations and their intensities. [ABSTRACT FROM AUTHOR]

Schistosomiasis is a major neglected parasitic disease that affects more than 265 million people worldwide and for which the control strategy consists of mass treatment with the only available drug, praziquantel. In this study, a series of new benzohydroxamates were prepared as potent inhibitors of Schistosoma mansoni histone deacetylase 8 (smHDAC8). Crystallographic analysis provided insights into the inhibition mode of smHDAC8 activity by these 3-amidobenzohydroxamates. The newly designed inhibitors were evaluated in screens for enzyme inhibitory activity against schistosome and human HDACs. Twenty-seven compounds were found to be active in the nanomolar range, and some of them showed selectivity toward smHDAC8 over the major human HDACs (1 and 6). The active benzohydroxamates were additionally screened for lethality against the schistosome larval stage using a fluorescence-based assay. Four of these showed significant dose-dependent killing of the schistosome larvae and markedly impaired egg laying of adult worm pairs maintained in culture. [ABSTRACT FROM AUTHOR]

A series of hydroxamates ( 4a–4l ) were prepared from p -aminobenzoic acid to inhibit HDAC8. The idea is to substitute rigid aromatic ring in place of less rigid piperazine ring of hydroxamates reported earlier by our group. It is expected to increase potency retaining the selectivity. Result obtained suggested that the modifications carried out retained the selectivity towards HDAC8 isoform and increasing the potency in very few cases. Increase in potency is also associated with variation in cap aryl region. Two compounds ( 4f & 4l ) were found to inhibit HDAC8 at concentrations (IC 50 ) less than 20 μM. [ABSTRACT FROM AUTHOR]

H2A.Z is an essential histone variant implicated in the regulation of key nuclear events. However, the metazoan chaperones responsible for H2A.Z deposition and its removal from chromatin remain unknown. Here we report the identification and characterization of the human protein ANP32E as a specific H2A.Z chaperone. We show that ANP32E is a member of the presumed H2A.Z histone-exchange complex p400/TIP60. ANP32E interacts with a short region of the docking domain of H2A.Z through a new motif termed H2A.Z interacting domain (ZID). The 1.48 Å resolution crystal structure of the complex formed between the ANP32E-ZID and the H2A.Z/H2B dimer and biochemical data support an underlying molecular mechanism for H2A.Z/H2B eviction from the nucleosome and its stabilization by ANP32E through a specific extension of the H2A.Z carboxy-terminal α-helix. Finally, analysis of H2A.Z localization in ANP32E−/− cells by chromatin immunoprecipitation followed by sequencing shows genome-wide enrichment, redistribution and accumulation of H2A.Z at specific chromatin control regions, in particular at enhancers and insulators. [ABSTRACT FROM AUTHOR]

This study aims to characterize the correlations between electric characteristics and selected structural features of newly designed Al/Cu laminated conductors manufactured via room temperature rotary swaging. After swaging, the laminates with diameters of 15 mm were subjected to two different post-process annealing treatments. Structure analyses performed to evaluate the effects of thermomechanical processing were performed via scanning and transmission electron microscopies. Electric conductivity and resistivity of the laminates were experimentally measured and numerically simulated using models designed according to the real conditions. The results showed that the electric resistivity was affected by the grain size, bimodal grains' distribution (where observed), the presence of twins, and, last but not least, dislocation density. Among the influencing factors were the area fractions of Al and Cu at the cross-sections of the of the laminated conductors, too. The results revealed that fabrication of the laminate via the technology of rotary swaging introduced more advantageous combinations of electric and mechanical properties than fabrication by conventional manufacturing techniques. The lowest specific electric resistivity of 20.6 Ωm × 10−9 was measured for the laminated conductor subjected to the post-process annealing treatment at 350 °C, which imparted significant structure restoration (confirmed by the presence of fine, equiaxed, randomly oriented grains). [ABSTRACT FROM AUTHOR]