1. Graft-versus-host disease of the CNS is mediated by TNF upregulation in microglia.
- Author
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Mathew, Nimitha R., Vinnakota, Janaki M., Apostolova, Petya, Erny, Daniel, Hamarsheh, Shaimaa, Andrieux, Geoffroy, Jung-Seok Kim, Hanke, Kathrin, Goldmann, Tobias, Chappell-Maor, Louise, El-Khawanky, Nadia, Ihorst, Gabriele, Schmidt, Dominik, Duyster, Justus, Finke, Jürgen, Blank, Thomas, Boerries, Melanie, Blazar, Bruce R., Jung, Steffen, and Prinz, Marco
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GRAFT versus host disease , *T helper cells , *DELETION mutation , *RNA analysis , *CENTRAL nervous system , *GRAFT copolymers - Abstract
Acute graft-versus-host disease (GVHD) can affect the central nervous system (CNS). The role of microglia in CNS-GVHD remains undefined. In agreement with microglia activation, we found that profound morphological changes and MHC-II and CD80 upregulation occurred upon GVHD induction. RNA sequencing-based analysis of purified microglia obtained from mice with CNS-GVHD revealed TNF upregulation. Selective TNF gene deletion in microglia of Cx3cr1creER Tnffl/- mice reduced MHC-II expression and decreased CNS T cell infiltrates and VCAM-1+ endothelial cells. GVHD increased microglia TGF-β-activated kinase-1 (TAK1) activation and NF-κB/p38 MAPK signaling. Selective Tak1 deletion in microglia using Cx3cr1creER Tak1fl/fl mice resulted in reduced TNF production and microglial MHC-II and improved neurocognitive activity. Pharmacological TAK1 inhibition reduced TNF production and MHC-II expression by microglia, Th1 and Th17 T cell infiltrates, and VCAM-1+ endothelial cells and improved neurocognitive activity, without blocking graft-versus-leukemia effects. Consistent with these findings in mice, we observed increased activation and TNF production of microglia in the CNS of GVHD patients. In summary, we prove a role for microglia in CNS-GVHD, identify the TAK1/TNF/MHC-II axis as a mediator of CNS-GVHD, and provide a TAK1 inhibitor-based approach against GVHD-induced neurotoxicity. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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