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Soil moisture (SM) plays an important role in regulating terrestrial–atmospheric water circulation and energy balance. Most of the existing studies have explored the dynamic patterns of SM based on experimental methods. However, the analysis of large-scale regions and long-term SM sequences was limited. Alternatively, satellite remote sensing data is a potential source for SM analysis for large-scale basins. Therefore, the SM data from the European Space Agency (ESA) Climate Change Initiative (CCI) from 2000 to 2015 is used in this paper to analyze the SM spatial-temporal changes in the Yellow River Basin (YRB). Further, the Normalized Difference Vegetation Index (NDVI) and meteorological data are used to explore the relationships between SM and NDVI, precipitation, air temperature, and wind speed, respectively. The results showed that the overall trend of SM in the YRB was decreasing from southeast to northwest during the past 16 years. The upper reaches of the YRB had shown a humid trend, with a value of 0.00047 m3·m−3·year−1, mainly due to the increase in precipitation; there was an obvious drought trend in the middle reaches of the YRB, especially in Shanxi Province and Henan Province, with a value of −0.00030 m3·m−3·year−1, which may be owed to vegetation greening increasing the soil evaporation. Overall, it is determined that the main factors influencing SM changes were NDVI and precipitation, followed by air temperature and wind speed. This study can provide a scientific basis for the spatial-temporal distribution characteristics and attributions of SM in the YRB over a long time series. [ABSTRACT FROM AUTHOR]