Search

The current study was conducted to determine the impact of immersion using garlic, sumac, and citric acid on certain qualitative characteristics of Japanese threadfin bream (Nemipterus japonicus) during frozen storage. Fish were immersed with 1% addition of each natural substance and stored at -18 C for 150 days. The results revealed that immersion with garlic, sumac, and citric acid prevented lipid oxidation and reduced the production of free fatty acids (FFA), volatile nitrogen bases (TVN), and thiobarbituric acid (TBA) in the groups treated with natural substances compared to untreated and water-immersed groups. The lowest pH value was 6.11 at day zero for the frozen fish, which increased to 6.98 at the end of storage for the same treatment. The lowest TVN, FFA, and TBA values were observed in the garlic-treated fish at day zero: 4.2mg nitrogen/100g fish, 0.12%, and 0.27mg malondialdehyde/100g fish, respectively. These values increased significantly during the final storage period to 29.4mg nitrogen/100g fish, 0.88%, and 5.41mg malondialdehyde/100g fish for the frozen fish only at 150 days of storage, with significant differences among treatments and storage periods (P<0.05). The lowest percentage of lost fluids was 2.33% for the garlic immersion treatment at day zero, while the highest value was 5.49% for the frozen fish only at 150 days. In conclusion, the study demonstrated the potential use of these substances in immersion as natural antioxidants and inhibitors of enzymatic, microbial, and quality loss. [ABSTRACT FROM AUTHOR]

Surimi industry produces a large quantity of byproducts that are currently being utilized to produce low‐value commodities. This study aims to extract gelatin from pink perch skin and bone obtained from the surimi industry using a green single‐step extraction method. In addition to using a green solvent, that is, acetic acid, the new method combines the multiple steps of pre‐treatment and hydrolysis into one single‐step extraction process. Response surface methodology was used to optimize extraction parameters (pH, temperature, and time) to maximize yield and l‐hydroxyproline (l‐hyp) content. The optimum condition for gelatin extraction was obtained at pH 3, 75°C, and 30 min. At optimum conditions, gelatin yield and l‐hyp content were observed to be 16.07% and 41.26 mg g−1, respectively. The gelatin obtained at optimized condition was further compared with commercial bovine gelatin (BG) in terms of chemical composition and textural, functional, and rheological properties. The results suggested that the optimized pink perch gelatin had higher protein content (92.06%), better gel strength (251.08 g), higher imino acid (18.01%), and improved textural and functional properties than the commercially available BG. The optimized single‐step gelatin extraction method from pink perch skin and bones is a promising, rapid, and efficient method for the production of good‐quality gelatin, which can be further used for the development of high‐value products such as food formulations. Practical Application: Fish gelatin is widely used in food product development. Most of the existing methods of the development of high‐value product such as gelatin, use multi‐step process and harsh mineral acid, therefore, are time‐consuming and harmful to the environment. This study provides a green single‐step gelatin extraction method that provides an efficient, rapid, and convenient method of gelatin extraction and a sustainable solution for fish industry byproduct utilization. The data obtained with this laboratory‐scale study provides a strong basis for scale‐up studies. [ABSTRACT FROM AUTHOR]

Holocolax nemipteri (Pillai, 1973) is redescribed based on specimens of the female recovered from the bucco-branchial cavity of the original host, the Japanese threadfin bream Nemipterus japonicus (Bloch, 1791) in Japan. Copepodid III sex undetermined of the species recovered from the same host is described for the first time in the genus. [ABSTRACT FROM AUTHOR]

In this study, chitosan coatings incorporated with propolis extract (PE) were utilized to extend the shelf-life of refrigerated Nemipterus japonicus fillets. Microbiological (total mesophilic bacteria (TMB) and total psychrotrophic count (TPC)), physicochemical (pH, total volatile bases nitrogen (TVB-N), 2-thiobarbituric acid reactive substances (TBARS), free fatty acid (FFA)) and sensory (color, odor and preference) characteristics of coated fillets were evaluated during 12 days. The results showed that incorporating PE into chitosan solution could significantly (P < 0.05) improve the quality of fish fillets. The results showed that the chitosan+PE samples reduced the extent of lipid oxidation, as judged by TBARS and FFA, suggesting that there is a synergistic effect between chitosan coating and propolis. Moreover, the application of chitosan coatings with PE improved the TVB-N and pH values of the Nemipterus japonicus samples significantly compared to untreated sample, thus extending the shelf life of Nemipterus japonicus fillet approximately >10 days. All wrapped fillets in treatment samples incorporated with PE had lower bacterial counts compared to control (P < 0.05). However, the bacterial counts of chitosan coating combined with PE were lower than those treated by chitosan or PE alone during the storage. The fillets treated with chitosan and PE had the best sensory properties. The increase (>10 days) in the shelf-life was attributed to the antioxidant and antimicrobial characteristics of chitosan and PE. These results confirmed that chitosan coating enriched with PE can be an improving method to reducing deterioration of refrigerated Nemipterus japonicus fillets. [ABSTRACT FROM AUTHOR]

Changes in total fatty acids in fillets of yellowstripe scad, Japanese threadfin bream, and salmon when applying different cooking methods were evaluated. All fish fillets (100 g fresh weight) were subjected to deep drying, grilling, baking in foil, and steaming. The results showed that deep frying of Japanese threadfin bream fillet significantly increased the total saturated fatty acid (955 mg/100 g) compared with the other cooking methods (499-612 mg/100 g). Baking in foil showed a significantly lower retention of total monounsaturated fatty acid in all fish fillets compared to the raw sample, especially yellowstripe scad with a total monounsaturated fatty acid content of 175 mg/100 g. Retention of DHA+EPA (mg/100 g) in yellowstripe scad fillet was found to be the highest by applying steaming method (112) compared to the raw fillet (119), followed by baking in foil (108), grilling (99), and deep frying (93). Steaming and baking in foil methods were able to retain the DHA and EPA content in the cooked fillets of all types of the studied fish compared to raw fillet. Deep frying and grilling methods showed a significant reduction of DHA and EPA contents in all fish fillets compared with steaming and baking in foil. The effect of different cooking methods was found to be significantly associated with the true retention values of DHA and EPA. In conclusion, steaming and baking in foil would be the best cooking methods for retention of DHA and EPA in yellowstripe scad fillet. [ABSTRACT FROM AUTHOR]

Proper and sensible formulation of ingredients in noodles facilitates its calorie cut down thus making it healthier. Fish being a source of cheap and quality protein can serve in the fortification of noodles. Hence, a study was done to formulate and develop fish noodles from Nemipterus japonicus. D-optimal mixture experimental design with 15 runs was formulated to optimise the different levels of ingredients for noodles. Influence of different ingredients, viz. fish mince, wheat and refined wheat flour (maida) and potato starch, on the physical, cooking and sensory properties of fish noodles was evaluated. The protein content of noodles increased with increase in the fish mince incorporation. Water absorption capacity (WAC) was found to be directly related to the potato starch concentration. Positive correlation was observed between noodle protein content and cooking time. Desirability function scores revealed a noodle combination of 45% fish mince, 47% wheat: maida (1:1) mix and 8% potato starch as optimum. [ABSTRACT FROM AUTHOR]

Background: Anisakid nematodes are common parasites of fish, mammals, fisheating birds, and reptiles with a worldwide distribution, causing diseases in human, fish and important economic losses. Methods: A preliminary epidemiological study was carried out on Anisakid nematodes larvae in some commercially important fish species to evaluate the anisakid nematode larvae from greater lizardfish, (Saurida tumbil), Japanese thread fin bream (Nemipterus japonicus), crocodile longtom (Tylosurus crocodilus crocodiles) and longfin trevally (Carangoides armatus) from the Persian Gulf of Iran. Result: The collected larvae were identified mainly as the third larval stage (L3) of Hysterothylacium larval type A, B and C, Anisakis sp., Raphidascaris sp., Pseudoterranova sp. and Philometra sp. (Nematoda: Philometridae). The prevalence of Anisakid larvae infection of examined fishes was 97.2% in N. japonicus, 90.3% in S. tumbil, 20.5% in crocodile longtom and 5.5% in longfin trevally. Anisakis type III for the first time was different from Anisakis type I and Anisakis type II. Discussion: Zoonotic anisakids by high prevalence in edible fish could be a health hazard for people. So health practices should be considered in these areas. [ABSTRACT FROM AUTHOR]

We evaluated genetic differentiation among ten presumed Japanese threadfin bream,Nemipterus japonicuspopulations along the coast of Peninsular Malaysia based on the partial sequence of the mitochondrial cytochromebgene (982 bp). Genetic divergences (Kimura-2 parameter) ranged from 0.5% to 0.8% among nine of the ten populations while these nine populations were 4.4% to 4.6% diverged from the Kuala Besar population located at the Northeast coast. The constructed Neighbour Joining (NJ) phylogenetic trees based on haplotypes showed the Kuala Besar population forming an isolated cluster. The Analysis of Molecular Variance (AMOVA) of the ten populationsa prioriassigned into four regions, revealed that most of the variation occurred within population with a fairly low but significant level of regional differentiation (FST = 0.07,p < 0.05, FSC = 0.00,p > 0.05 and FCT = 0.07,p < 0.05) attributed to the Kuala Besar population.pValue after Bonferroni correction revealed that only pairwise FSTvalues involving the Kuala Besar population with the other nine populations were significant. Thus, this study revealed that theN. japonicuspopulations off Peninsular Malaysia were panmictic. However, the Kuala Besar population, although morphologically identical was composed of a genetically discrete taxon from the rest. These findings are important contributions in formulating sustainable fishery management policies for this important fishery in Peninsular Malaysia. [ABSTRACT FROM AUTHOR]

A total of 188 fish samples of Nemipterus japonicus (Bloch, 1791) were collected from north west of the Arabian Gulf (latitudes 48° 44′ to 48° 46′; longitude 29° 46′ to 29° 47′) during the period from January 2011 till January 2012. The copepod Caligus epinepheli Yamaguti, 1936 was recorded from the gills during most months of the year. Descriptions of adult parasites and chalimi (larval stages of the parasites) were included. Females body length range was 1.38-2.55 (2.16) mm. Egg sac length 0.99-1.80 (1.31) mm, the number of eggs ranged from 13-22 (17) in each egg sac. The range of the males body length was 1.45-1.73 (1.59) mm. Statistical analysis showed no significant differences in the infestations of both sexes of the host (except in fish group with body length 18-21cm.).The highest incidence of infestation was recorded during March and June (57.1%), while the lower level of infestation was recorded during December (7.6%). The high intensity of infestation was recorded during April (10), while the lowest intensity of infestation was recorded in December (1). [ABSTRACT FROM AUTHOR]

In the present study, bioactive peptide was isolated from protein hydrolysate of Nemipterus japonicus backbone hydrolysed by trypsin. Functional properties like solubility, emulsifying, and foaming capacities of protein hydrolysate were governed by pH. Protein hydrolysate was fractionated by DEAE XK 26/20 anion exchange chromatography followed by G-25 gel filtration chromatography. The fractions collected were tested for radical scavenging properties on DPPH, hydroxyl, and superoxide radicals by electron spin resonance spectrometer. Amino acid composition of a potent fraction was rich in glutamic acid (21.20 g/100 g), lysine (37.50 g/100 g), aspartic acid (5.8 g/100 g), arginine (5.4 g/100 g), and glycine (2.84 g/100 g). Moreover, the potent fraction showed no cytotoxicity on Vero cell lines till 0–100 μg/ml with CC50 = 94 μg/ml, whereas it exerted a significant antitumor property against HepG2 cell line with an IC50 value of 61.1 μg/ml. [ABSTRACT FROM PUBLISHER]

In the present study, protein of muscles of commercially important marine fishes Nemipterus japonicus and Exocoetus volitans were extracted by trypsin and their hydrolysis conditions viz., temperature, time, and enzyme to substrate concentration on degree of hydrolysis were studied by response surface methodology. The optimum values for N. japonicus was found as temperature, 30°C, hydrolysis time of 100 min an enzyme/substrate concentration of 1.59% whereas, for E. volitans muscle protein, optimum hydrolysis conditions were temperature, 30°C, hydrolysis time of 115 min and enzyme/substrate concentration of 1.67%. Furthermore, amino acid sequence of antioxidant peptides derived after chromatographic purification was identified by ESI-MS/MS. The analysis of peptides showed sequences as Glu-Ser-Asp-Arg-Pro (620.3 Da) and Gly-Trp-Met-Gly-Cys-Trp (747.3) for N. japonicus and E. volitans muscle, respectively. The peptides contained important antioxidant amino acids and acted as good antioxidant peptides to scavenge free radicals. [ABSTRACT FROM AUTHOR]

A specimen of the Japanese threadfin bream Nemipterus japonicus was recorded for the first time from the Mediterranean Sea. The presence of this Indo-Pacific fish in the Mediterranean is evidently due to migration from the Red Sea via the Suez Canal. [ABSTRACT FROM AUTHOR]

Catch rates and sizes of Saurida undosquamis and Nemipterus japonicus were more variable during daytime. But, Pomadasys stridens were more variable in night tows. Catch rates of S. undosquamis and N. japonicus were higher during daytime and of significantly small mean fish size. Inversely, P. stridens had significant higher catch rates and the fish caught were smaller in night tows.Variability of catch rate and size of Penaeus semisulcatus and Penaeus japonicus was higher at night. Daytime catch rates were significantly lower and of smaller mean shrimp size. Penaeus latisulcatus was available only at night. [Copyright &y& Elsevier]

The Japanese threadfin bream N. japonicus (Bloch) (Perciformes: Nemipteridae) is an important marine food fish in Asia. However, our present knowledge of the occurrence of its nematode parasites is still limited. In the present study, the species composition and infection rate of ascaridoid nematodes in N. japonicus from the South China Sea, were studied for the first time. Five ascaridoid species, namely Anisakis typica (L3), Hysterothylacium amoyense (L3), Hysterothylacium sp. IV-A (L3), adult of H. thalassini and Raphidascaris lophii (L3), were identified using integrative taxonomy. Hysterothylacium amoyense was the most prevalent species (prevalence 47.2%, mean intensity 14.9 ± 17.1). Hysterothylacium thalassini and R. lophii were reported in the Japanese threadfin bream for the first time. Two different genotypes of A. typica (overall prevalence of 3.4%; mean intensity 1.7 ± 0.9) were found in the South China Sea for the first time. The unique restriction polymorphism patterns of three species of Hysterothylacium are provided for rapid diagnosis. Our present results indicate that RFLP analysis of ITS region, using the restriction enzymes Hha I and Rsa I, represents a simple and practical method for large-scale surveys of Hysterothylacium for seafood industry. Unlabelled Image • Five ascaridoids were identified. • H. amoyense (L3) was the most prevalent species. • Two different genotypes of A. typica were found. [ABSTRACT FROM AUTHOR]

The complete mitochondrial sequence of the Japanese threadfin bream,Nemipterus japonicushas been determined. The circle genome is 16,995 bp in size, and consists of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a control region. The gene order and composition ofN. japonicuswas similar to that of most other teleosts. The base composition of H-strand is 28.11% (A), 28.02% (T), 16.64 % (G) and 27.24 % (C), with an AT content of 56.12%. All genes are encoded on the heavy strand with the exception ofND6and eight tRNA genes. The mitochondrial genome ofN. japonicuspresented will be in favor of resolving phylogenetic relationships within the family Nemipteridae and the Perciformes. [ABSTRACT FROM AUTHOR]