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Onshore wind power is increasingly expanding to meet global and national goals to increase renewable, clean, and fossilfree energy production. In many countries and regions, however, historical and current land use is extensive, and the expansion of wind power has to be well-tuned to avoid risking irreversible legacy losses of existing and traditional land uses, landscape values, and cultures. Hence, assessments of the siting premises of current and forecasted expansion of wind power are strongly needed as a basis for sustainable planning. We present a study from alpine to temperate biomes in Sweden, where an ambitious onshore wind power expansion strategy has been put in place and will result in Swedish landscapes that are typified by wind power. We explored the existing legal framework--i.e., the national interest for wind power according to the Swedish Environmental Code--concerning the spatial interaction with other national interests for nature conservation, landscape values, and other land uses, and the land cover, landowner, and formally protected areas distribution within wind power sites and in their proximity. We found that the national interest framework does not provide sufficient guidance for locating wind power to avoid spatial overlap with conflicting interests and values. Furthermore, our analysis revealed that wind power is located mainly in forest-dominated landscapes, and on lands where private forest companies are the dominant owners but where the proportion of public and non-industrial private ownership increases in the near surroundings. Finally, we found that large areas of formally protected areas are within the proximate areas influenced by wind power. As an extensive onshore wind power expansion is already going on, and an even more extensive expansion is projected, the ways forward toward a sustainable wind power expansion calls for integrated landscape planning approaches that are based on comprehensive assessments of existing interests and values. [ABSTRACT FROM AUTHOR]

Hunting is a widespread but often overlooked land-use activity, providing major benefits to society. Hunting takes place in most landscapes, yet it remains unclear which types of landscapes foster or dampen hunting-related services, and how hunting relates to other land uses. A better understanding of these relationships is key for sustainable land-use planning that integrates wildlife management. This is particularly urgent for Europe, where wildlife populations are increasing. Focusing on Sweden, we explored the spatial associations among hunting, agriculture, and forestry to identify archetypical combinations of these land uses. Specifically, we combined indicators on the extent and intensity of agriculture and forestry, with data on hunting bags for 63 game species using selforganizing maps, a non-parametric clustering approach. We identified 15 typical bundles of co-occurring land uses at the municipality level across Sweden. The harvest of forest grouse, bears, and moose co-occurred with forestry in northern Sweden, whereas the harvest of small game, different deer species, and wild boar co-occurred with agriculture across southern Sweden, reflecting species' biology, environmental factors, and management. Our findings also highlight the strength of associations among hunting and other land uses. Importantly, we identified large areas in central Sweden where harvest of game was below average, possibly indicating that intensity of hunting is out of balance with that of agriculture or forestry, potentially fostering conflict between wildlife and land use. Collectively, our results suggest that (1) hunting should be considered a major land use that, in Sweden, is more widespread than agriculture and forestry; (2) land-use planning must therefore integrate wildlife management; and (3) such an integration should occur in a regionalized manner that considers social-ecological context. Our approach identifies a first spatial template within which such context-specific land-use planning, aiming at aligning wildlife and diverse land uses, can take place. [ABSTRACT FROM AUTHOR]

Purpose: Three‐dimensional (3D) printing allows for the fabrication of medical devices with complex geometries, such as soft actuators and robots that can be used in image‐guided interventions. This study investigates flexible and rigid 3D‐printing materials in terms of their impact on multimodal medical imaging. Methods: The generation of artifacts in clinical computer tomography (CT) and magnetic resonance (MR) imaging was evaluated for six flexible and three rigid materials, each with a cubical and a cylindrical geometry, and for one exemplary flexible fluidic actuator. Additionally, CT Hounsfield units (HU) were quantified for various parameter sets iterating peak voltage, x‐ray tube current, slice thickness, and convolution kernel. Results: We found the image artifacts caused by the materials to be negligible in both CT and MR images. The HU values mainly depended on the elemental composition of the materials and applied peak voltage was ranging between 80 and 140 kVp. Flexible, nonsilicone‐based materials were ranged between 51 and 114 HU. The voltage dependency was less than 29 HU. Flexible, silicone‐based materials were ranged between 60 and 365 HU. The voltage‐dependent influence was as large as 172 HU. Rigid materials ranged between −69 and 132 HU. The voltage‐dependent influence was <33 HU. Conclusions: All tested materials may be employed for devices placed in the field of view during CT and MR imaging as no significant artifacts were measured. Moreover, the material selection in CT could be based on the desired visibility of the material depending on the application. Given the wide availability of the tested materials, we expect our results to have a positive impact on the development of devices and robots for image‐guided interventions. [ABSTRACT FROM AUTHOR]

Objectives: To assess the risks for implant users with copper-containing intrauterine devices (IUDs) during MR and CT examinations.Methods: A tissue-mimicking phantom suitable for all experiments within this study was developed. Seven different types of copper IUDs were evaluated. Heating and dislocation of each IUD were investigated at two clinically relevant positions in 1.5 T and 3 T MR scanners. Artifacts in the field of view caused by each tested IUD were determined for clinical MR and CT imaging.Results: No significant heating of any tested IUD was detected during MR measurements. The temperature increase was less than 0.6 K for all IUDs. Neither angular deflection nor translation of any IUD was detected. Artifacts in MR images were limited to the very vicinity of the IUDs except for one IUD containing a steel-visualizing element. Streaking artifacts in CT were severe (up to 75.5%) in the slices including the IUD.Conclusion: No significant risk possibly harming the patient was determined during this phantom study, deeming MR examinations safe for women with an implanted copper IUD. Image quality was more impaired for CT than for MR imaging and needs careful consideration during diagnosis.Key Points: • Risk assessment of copper-containing IUDs with regard to heating, dislocation, and artifacts during MR and CT imaging. • Neither significant heating nor dislocation was determined in MR; image quality was more impaired for CT than for MR imaging and needs careful consideration during diagnosis. • The tested IUDs pose no additional risks for implant users during MR and CT examinations. [ABSTRACT FROM AUTHOR]

This work demonstrates a new method for the generation of mechanical shear wave during magnetic resonance elastography (MRE) that creates greater forces at higher vibrational frequencies as opposed to conventionally used pneumatic transducers. We developed an MR-compatible pneumatic turbine with an eccentric mass that creates a sinusoidal centrifugal force. The turbine was assessed with respect to its technical parameters and evaluated for MRE on a custom-made anthropomorphic prostate phantom. The silicone-based tissue-mimicking materials of the phantom were selected with regard to their complex shear moduli examined by rheometric testing. The tissue-mimicking materials closely matched human soft tissue elasticity values with a complex shear modulus ranging from 3.21 kPa to 7.29 kPa. We acquired MRE images on this phantom at 3 T with actuation frequencies of 50, 60 Hz, 70 Hz, and 80 Hz. The turbine generated vibrational wave amplitudes sufficiently large to entirely penetrate the phantoms during the feasibility study. Increased wave length in the stiffer inclusions compared to softer background material were detected. Our initial results suggest that silicone-based phantoms are useful for the evaluation of elasticities during MRE. Furthermore, our turbine seems suitable for the mechanical assessment of soft tissue during MRE. [ABSTRACT FROM AUTHOR]

ABSTRACT: Vehicle collisions with large wildlife provide a serious risk of severe personal injuries. In some countries, there is a common belief that hunting increases this risk because it leads to more active animals and increases roads crossings. We analyzed movements of moose (Alces alces) and frequency of moose road crossings during fall to test if moose altered their behavior during hunting periods compared to other times. We analyzed global positioning system (GPS) data from 172 free‐ranging adult moose (140 females, 32 males) between the end of August and the middle of October 2004–2013, in 7 different areas along Sweden's latitudinal gradient of 57°N–67°N. Female moose either decreased or did not change their movement rates as fall proceeded, and they did not cross roads more in any weeks of the hunting season. Male moose became more active between mid‐September and the beginning of October, which corresponds to the time before the hunting period in southern Sweden and the temporary hunting closure in northern Sweden. There was no overall change in moose movements in relation to hunting activity during fall regardless of region or sex. We found that number of road crossings correlated positively with the rutting period in male moose. Thus, the riskier periods for traffic during fall during the hunting period, based on moose movement, coincided with the rutting activity. We suggest that mitigation actions to reduce moose‐vehicle collisions should make drivers more aware of changes in movement behavior in moose during the rutting season, particularly in southern Sweden. © 2018 The Wildlife Society. [ABSTRACT FROM AUTHOR]

Abstract: Human–wildlife conflicts like wildlife–vehicle collisions pose major challenges for the management and conservation of mobile wildlife in human-dominated landscapes, particularly when large species are involved. Mitigation measures to reduce risk of collisions may be based on information given by wildlife movement and collision data. To test whether movement and collision data indicate different spatiotemporal risk zones, we predicted year-around probabilities of road-crossings of GPS-marked female moose (Alces alces) (n =102), and compared them with spatiotemporal patterns of police recorded moose-vehicle collisions (n =1158). Probability of moose road-crossings peaked in May, June, and between mid November and the beginning of January, i.e. during moose migration. Moose-vehicle collisions were more likely during autumn and winter. Comparing environmental attributes of crossing and collision sites showed significant differences. The likelihood of collisions increased with the abundance of human-modified areas and higher allowed speed, and was lower on forest roads. We found that animal movement data alone are insufficient to predict collision risk zones, while analyses of collision data alone overestimate the collision risk in certain habitats. Our findings suggest that higher collision risk is largely due to low light and poor road surface conditions rather than to more animal road-crossings. This suggests that efforts to reduce wildlife collisions should focus on driver attitudes and road conditions rather than animal movement, and any efforts to model the collision risk will require actual collision data, and not just movement data. [Copyright &y& Elsevier]

Studies of free-ranging wildlife often involve animal capture and fitting of tracking devices. Capturing wildlife may result in behavioral alterations. Thus, there is a need to evaluate the effects of capture on study animals to identify potential biases influencing the research. We assessed the short-term response of 15 GPS/GSM-collared adult female moose ( (L., 1758)) and immobilization and handling by comparing moose rates of movement and net square displacement before and after recapture. Moose were more active up to 7 h and increased their spatial displacement for 4.5 days after recapture compared with movement patterns before recapture. Opposite to our predictions, moose did not reduced their movement rates after their initial displacement following capture and recovery, i.e., moose did not show any indication for a residual effect. We recommend using individuals as their own controls in analyses of capture impacts to account adequately for individual behavioral differences. We recommend omitting data of at least the first 5 days following capture for analyses of moose movement and distribution. [ABSTRACT FROM AUTHOR]

Continual expansion of human development and recreational activity into previously undisturbed environments and wildlife habitat highlights the need for better understanding of behav- ioral impacts of human-induced disturbances on wildlife, especially where harvest is the main source of mortality. In a controlled field experiment in northern Sweden, we exposed 29 adult free-ranging GPS-collared female moose (Alces alces) to either off-trail hiking or snowmobiling activity to study individual response to non-lethal human activities. Both experimental disturbances resulted in signifi- cant increase in movement rates and diurnal activity ranges, and prompted moose to leave the area. Movement rates were elevated for 1 and 2 h following hiking and snowmobiling, respectively. We found that the overall moose response to human-induced disturbances was short in duration, suggesting negligible effect on the overall energy budget of moose in good condition when disturbances occur at moderate frequency. [ABSTRACT FROM AUTHOR]

Helicopters are used for numerous wildlife management and research purposes, but can alter wildlife behaviour and influence baseline data collection. We investigated reactions of GPS-collared moose Alces alces and brown bears Ursus arctos to short-term helicopter approaches by researchers. Moose responded with up to 10 times greater movement rates for up to two hours following a helicopter approach and moved into more rugged terrain. Brown bears decreased their speed and remained within similar habitat types and terrain. The movements were influenced only about two hours and did not influence the size of the activity areas. Contrary to our predictions, brown bears responded with a somewhat calmer response than moose, illustrating response differences in large herbivores and carnivores. This difference in response might be because brown bears are actually less disturbed than moose by direct helicopter approaches or because of a difference in tactical behaviour between brown bears and moose following disturbance. Researchers and managers should thus be cautious in using knowledge from one species to predict or perceive disturbance response in another species or taxa. [ABSTRACT FROM AUTHOR]

Introduction: Magnetic resonance elastography (MRE) is an MR imaging method for the quantification of spatial stiffness of soft tissues using mechanically induced dynamic shear waves. In some applications, the penetration of shear waves can be limited through attenuation and shadowing of the waves. In order to increase the actuator performance, we present a dual driver approach to compensate for shear wave attenuation and to achieve better coverage over the entire region of interest. Materials and Methods: (a) We designed pneumatic turbines that created a sinusoidal centrifugal force due to an eccentric weight. Two turbines were connected in‐phase with each eccentric weight having the same angular position relative to its pivot point. (b) We developed a tissue elasticity mimicking abdominal phantom. (c) The phantom served as a test object to investigate the feasibility to generate shear waves at two surface origins with the dual actuator system and to compare it against a single actuation setup. Results and Discussion: A stable phase relationship of the shear waves generated by the turbines was achieved as the positions of the eccentric weights were mechanically fixed. The abdominal phantom yielded sufficient MR signal. Liver and rib cage were clearly visible in MR imaging. The shear waves generated by the dual turbine propagated through the region of interest. Our turbine design is reproducible through 3D printing and can be integrated into existing clinical equipment for 1.5 T and 3 T scanners. [ABSTRACT FROM AUTHOR]

The careful selection of ungulate calving sites to improve offspring survival is vital in the face of predation. In general, there is limited knowledge to which degree predator presence and prey's individual experience shape the selection of calving sites. Predator presence influences the spatiotemporal risk of encountering a predator, while individual experiences with previous predation events shape perceived mortality risks. We used a multi‐year movement dataset of a long‐lived female ungulate (moose, Alces alces, n = 79) and associated calf survival to test how predator presence (i.e., encounter risk) and females' individual experiences with previous calf mortality events affected their calving site selection and site fidelity. Using data from areas with and without Scandinavian brown bear (Ursus arctos) predation, we compared females' calving site selection using individual‐based analyses. Our findings suggest two things. First, bear presence influences calving site selection in this solitary living ungulate. Females in areas with bears were selected for higher shrub and tree cover and showed lower site fidelity than in the bear‐free area. Second, the individual experience of calf loss changes females' selection the following year. Females with lost calves had a lower site fidelity compared to females with surviving calves. Our findings suggest that increased vegetation cover may be important for reducing encounter risk in bear areas, possibly by improving calf concealment. Lower site fidelity might represent a strategy to make the placement of calving sites less predictable for predators. We suggest that bear presence shapes both habitat selection and calving site fidelity in a long‐lived animal, whereas the effect of individual experience with previous calf loss varies. We encourage further research on the relevance of female experience on the success of expressed anti‐predator strategies during calving periods. [ABSTRACT FROM AUTHOR]

Although the advent of high‐resolution GPS tracking technology has helped increase our understanding of individual and multispecies behavior in wildlife systems, detecting and recording direct interactions between free‐ranging animals remains difficult. In 2023, we deployed GPS collars equipped with proximity sensors (GPS proximity collars) on brown bears (Ursus arctos) and moose (Alces alces) as part of a multispecies interaction study in central Sweden. On 6 June, 2023, a collar on an adult female moose and a collar on an adult male bear triggered each other's UHF signal and started collecting fine‐scale GPS positioning data. The moose collar collected positions every 2 min for 89 min, and the bear collar collected positions every 1 min for 41 min. On 8 June, field personnel visited the site and found a female neonate moose carcass with clear indications of bear bite marks on the head and neck. During the predation event, the bear remained at the carcass while the moose moved back and forth, moving toward the carcass site about five times. The moose was observed via drone with two calves on 24 May and with only one remaining calf on 9 June. This case study describes, to the best of our knowledge, the first instance of a predation event between two free ranging, wild species recorded by GPS proximity collars. Both collars successfully triggered and switched to finer‐scaled GPS fix rates when the individuals were in close proximity, producing detailed movement data for both predator and prey during and after a predation event. We suggest that, combined with standard field methodology, GPS proximity collars placed on free‐ranging animals offer the ability for researchers to observe direct interactions between multiple individuals and species in the wild without the need for direct visual observation. [ABSTRACT FROM AUTHOR]

Collisions with wild ungulates are an increasing traffic safety issue in boreal regions. Crashes involving smaller-bodied deer species usually lead to vehicle damage only, whereas collisions with a large animal, such as the moose, increase the risk of personal injuries. It is therefore important to understand both the factors affecting the number of moose-vehicle collisions (MVCs) and the underlying causes that turn an MVC into an accident involving personal injuries or fatalities. As a basis for temporal mitigation measures, we examined the annual and monthly variation of MVCs with and without personal injuries. Using a 22-year-long (1990–2011) time series from Finland, we tested the effect of moose population density and traffic volume on the yearly number of all MVCs and those leading to personal injuries. We also examined the monthly distribution of MVCs with and without personal injuries, and contrasted the Finnish findings with collision data from Sweden (years 2008–2010) and Norway (years 2008–2011). Both moose population abundance indices and traffic volume were positively related to the yearly variation in the number of MVCs in Finland. The proportion of MVCs involving personal injuries decreased during our 22-year study period. The monthly distribution of all MVCs peaked during the autumn or winter depending on country, while MVCs involving personal injury peaked in summer. Our study indicates that efforts to reduce MVCs involving personal injuries need to address driver awareness and attitudes during summer, despite most MVCs occurring in autumn or winter. [ABSTRACT FROM AUTHOR]

Differences in botanical diet compositions among a large number of moose faecal samples collected during winter correlated with the nutritional differences identified in the same samples (Mantel‐r = 0.89, p = 0.001), but the nutritional differences were significantly smaller (p < 0.001).Nutritional geometry revealed that moose mixed Scots pine Pinus sylvestris and Vaccinium spp. as nutritionally complementary foods to reach a nutritional target resembling Salix spp. twigs, and selected for Salix spp. browse (Jacob's D > 0).Available protein (AP) and total non‐structural carbohydrates (TNC) were significantly correlated in observed diets but not in hypothetical diets based on food availability.The level of Acetoacetate in moose serum (i.e. 'starvation') was weakly negatively associated with digestibility of diets (p = 0.08) and unrelated to increasing AP:TNC and AP:NDF ratios in diets (p > 0.1).Our study is the first to demonstrate complementary feeding in free‐ranging moose to attain a nutritional target that has previously been suggested in a feeding trial with captive moose. Our results add support to the hypothesis of nutritional balancing as a driver in the nutritional strategy of moose with implications for both the management of moose and food resources. Read the free Plain Language Summary for this article on the Journal blog. [ABSTRACT FROM AUTHOR]

Aim: Several large‐mammal species in Europe have recovered and recolonized parts of their historical ranges. Knowing where suitable habitat exists, and thus where range expansions are possible, is important for proactively promoting coexistence between people and large mammals in shared landscapes. We aimed to assess the opportunities and limitations for range expansions of Europe's two largest herbivores, the European bison (Bison bonasus) and moose (Alces alces). Location: Central Europe. Methods: We used large occurrence datasets from multiple populations and species distribution models to map environmentally suitable habitats for European bison and moose across Central Europe, and to assess human pressure inside the potential habitat. We then used circuit theory modeling to identify potential recolonization corridors. Results: We found widespread suitable habitats for both European bison (>120,000 km2) and moose (>244,000 km2), suggesting substantial potential for range expansions. However, much habitat was associated with high human pressure (37% and 43% for European bison and moose, respectively), particularly in the west of Central Europe. We identified a strong east–west gradient of decreasing connectivity, with major barriers likely limiting natural recolonization in many areas. Main conclusions: We identify major potential for restoring large herbivores and their functional roles in Europe's landscapes. However, we also highlight considerable challenges for conservation planning and wildlife management, including areas where recolonization likely leads to human–wildlife conflict and where barriers to movement prevent natural range expansion. Conservation measures restoring broad‐scale connectivity are needed in order to allow European bison and moose to recolonize their historical ranges. Finally, our analyses and maps indicate suitable but isolated habitat patches that are unlikely to be colonized but are candidate locations for reintroductions to establish reservoir populations. More generally, our work emphasizes that transboundary cooperation is needed for restoring large herbivores and their ecological roles, and to foster coexistence with people in Europe's landscapes. [ABSTRACT FROM AUTHOR]

Partial migration, whereby a proportion of a population migrates between distinct seasonal ranges, is common throughout the animal kingdom. However, studies linking existing theoretical models of migration probability, with empirical data are lacking. The competitive release hypothesis for partial migration predicts that due to density‐dependent habitat selection, the proportion of migrants increases as the relative quality and size of the seasonal range increases, but decreases with increasing migration cost and population density. To test this prediction, we developed a quantitative framework to predict the proportion of migrants, using empirical data from 545 individually GPS‐marked moose Alces alces from across Fennoscandia, spanning latitudes of 56° to 68°N. Moose contracted their ranges to common and spatially limited winter areas (typically at lower elevation), but expanded them during summer due to an increase in suitable habitat (at highland ranges). As predicted from our model, a better and larger highland range relative to the lowland range corresponded to a higher proportion of migrants in an area. Quantitative predictions coupling the balance of habitat availability of seasonal ranges with the probability of migrating in a large herbivore is a necessary step towards an enhanced understanding of the mechanisms underlying migration at the population level. [ABSTRACT FROM AUTHOR]

Conservation of biodiversity requires in-depth knowledge of trait-environment interactions to understand the influence the environment has on species assemblages. Saproxylic beetles exhibit a wide range of traits and functions in the forest ecosystems. Understanding their responses to surrounding environment thus improves our capacity to identify habitats that should be restored or protected. We investigated potential interactions between ecological traits in saproxylic beetles (feeding guilds and habitat preferences) and environmental variables (deadwood, type and age of surrounding forest). We sampled beetles from 78 plots containing newly created high stumps of Scots pine and Silver birch in boreal forest landscapes in Sweden for three consecutive years. Using a model based approach, our aim was to explore potential interactions between ecological traits and the surrounding environment at close and distant scale (20 m and 500 m radius). We found that broadleaf-preferring beetle species are positively associated with the local broadleaf-originated deadwood and broadleaf-rich forests in the surrounding landscapes. Conifer-preferring species are positively associated with the local amount of coniferous deadwood and young and old forests in the surrounding landscape. Fungivorous and predatory beetles are positively associated with old forests in the surrounding landscapes. Our results indicate that both local amounts of deadwood and types of forests in the landscape are important in shaping saproxylic beetle communities. We particularly highlight the need to increase deadwood amounts of various qualities in the landscape, exempt older forests from production and to increase broadleaf-rich habitats in order to meet different beetle species' habitat requirements. Trait responses among saproxylic beetles provide insights into the significance of broadleaf forest and dead wood as essential attributes in boreal forest restoration, which helps conservation planning and management in forest landscapes. • Trait – environment interactions of saproxylic beetles are investigated. • Broadleaf-preferring beetles thrive with local broadleaved deadwood. • Broadleaf-rich forests contribute to the abundance of broadleaf-preferring beetles. • Fungivores and predators positively interact with old forests in the landscape. • Deadwood increase and a 'broadleafication' of boreal forests need to be implemented. [ABSTRACT FROM AUTHOR]

Understanding a species' feeding ecology is essential for successful management and conservation, because food abundance can influence body mass, survival, reproductive success, movements, and habitat use. We describe annual and seasonal variations in the diet of brown bears Ursus arctos in southcentral Sweden, based on analysis of 527 fecal samples from 1994-1996 and 2000-2001. There was distinct seasonal variation in most of the 26 food items we documented. Ungulates, predominantly moose Alces alces, and insects comprised most of the estimated dietary energy content in spring and summer. Insects were represented almost entirely by ants, of which Formica spp. and Camponotus herculeanus were the most common. During autumn, berries dominated the diet. The most important berry species were bilberry Vaccinium myrtillus, crowberry Empetrum hermaphoditum and lingonberry V. vitis-idaea. We determined berry availability by inventorying 308 random plots three times for two consecutive years. These three berries occurred with great spatial, seasonal and annual variation in abundance. The bears showed the strongest positive preference for bilberries, a lesser positive preference for crowberries, but no preference for lingonberries. The proportion of berries in the autmn diet was stable between years, but the relative importance of the species changed, indicating that bears switched to crowberries when bilberries were less abundant. The effects of predicted future climatic change might have severe effects on the availability of the berries, which is the only important food available for fat acquisition prior to hibernation. [ABSTRACT FROM AUTHOR]

Gestation and lactation have high energetic requirements. Up to three-fourths of the gestation period in moose (Alces alces) overlaps with the food-scarce period in winter. During this period, moose deal with the limited forage resources available through hypometabolism with decreased heart rate and body temperature (T b). Body temperature is also an indicator of oestrus, pregnancy and parturition, which is well documented in several domestic species. In this study, we sought to determine if moose displayed a similar T b pattern during pregnancy and parturition to domesticated ruminants, and if we could detect parturition by combining T b and activity data. We studied the T b pattern of 30 free-ranging adult female moose (≥1.5 years old), equipped with ruminal temperature loggers and GPS collars. We documented a 0.13–0.19°C higher T b in pregnant compared to non-pregnant moose, depending on the study area with the T b difference increasing along a south-north gradient, and a drop in T b and in activity when parturition was imminent. Detection of parturition was highly successful when combining T b and activity data with an accuracy of 91.5%. Our findings demonstrate that T b responses to pregnancy and parturition in a wild capital-breeding ruminant are similar to those of domesticated ruminants. • Body temperature in female moose varies between reproductive states. • Pregnant moose have a higher body temperature than non-pregnant females. • The body temperature of female moose drops when parturition is imminent. • Combined use of body temperature and activity data allows successful detection of parturition. [ABSTRACT FROM AUTHOR]

Background Unlike metastatic colorectal cancer (CRC) there are to date few reports concerning the predictive value of molecular biomarkers on the clinical outcome in stage II/III CRC patients receiving adjuvant chemotherapy. Aim of this study was to assess the predictive value of proteins related with the EGFR-and VEGFR-signalling cascades in these patients. Methods The patients' data examined in this study were from the collective of the 5-FU/FA versus 5-FU/FA/irinotecan phase III FOGT-4 trial. Tumor tissues were stained by immunohistochemistry for VEGF-C, VEGF-D, VEGFR-3, Hif-1 α, PTEN, AREG and EREG expression and evaluated by two independent, blinded investigators. Survival analyses were calculated for all patients receiving adjuvant chemotherapy in relation to expression of all makers above. Results Patients with negative AREG and EREG expression on their tumor had a significant longer DFS in comparison to AREG/EREG positive ones(p< 0.05). The benefit on DFS in AREG- /EREG- patients was even stronger in the group that received 5-FU/FA/irinotecan as adjuvant treatment(p=0.002). Patients with strong expression of PTEN profited more in terms of OS under adjuvant treatment containing irinotecan(p< 0.05). Regarding markers of the VEGFR-pathway we found no correlation of VEGF-C- and VEGFR-3 expression with clinical outcome. Patients with negative VEGF-D expression had a trend to live longer when treated with 5-FU/FA(p=0.106). Patients who were negative for Hif-1 α, were disease-free in more than 50% at the end of the study and showed significant longer DFS-rates than those positive for Hif-1 α (p=0.007). This benefit was even stronger at the group treated with 5-FU/FA/irinotecan(p=0.026). Finally, AREG-/EREG-/PTEN+ patients showed a trend to live longer under combined treatment combination. Conclusions The addition of irinotecan to adjuvant treatment with 5-FU/FA does not provide OS or DFS benefit in patients with stage II/III CRC. Nevertheless, AREG/EREG negative, PTEN positive and Hif-1 α negative patients might profit significantly in terms of DFS from a treatment containing fluoropyrimidines and irinotecan. Our results suggest a predictive value of these biomarkers concerning adjuvant chemotherapy with 5-FU/FA +/- irinotecan in stage II/III colorectal cancer. [ABSTRACT FROM AUTHOR]