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The objective of this study was to characterize the insoluble and soluble dietary fiber present in Argentine and Italian artichoke (Cynara scolymus) by-products by comparing chemical analysis and ATR-FTIR spectroscopy. Non-edible bracts of Argentine (AR) and Italian (Benevento (BN) and Sicily (SC)) artichoke cultivars were employed. The soluble and insoluble dietary fibers were extracted by physical procedures and determined by chemical, attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy, and chemometric analysis (principal components analysis, PCA). No differences in total dietary fiber content between AR and SC samples were observed, although they both showed higher values than BN. With respect to insoluble fiber, this fraction represents 82.5%, 63.5%, and 55.2% of the total dietary fiber for BN, SC and AR, respectively. Fibers from AR presented different compositions and structures, as determined by ATR-FTIR, compared to those of the Italian cultivars (BN and SC). Comparing the results of dietary fiber measured by ezymogravimetric assay with those obtained by ATR-FTIR and PCA, we conclude that it is possible to discriminate samples that contain different kinds of fiber using ATR-FTIR. [ABSTRACT FROM AUTHOR]

Abstract: The aim of this study was to determine the best synbiotic combination (based on growth and short‐chain fatty acids production) between Enterococcus faecalis and eight prebiotics. Based on the results of in vitro studies, E. faecalis + galactooligosaccharide (EGOS) and E. faecalis + mannanoligosaccharide (EMOS) were selected as synbiotics. A 126‐day feeding trial was conducted to compare the effects of prebiotics, probiotic and synbiotics on the growth indices, In vivo ADC of nutrients, digestive enzymes, hemolymph indices and finally, biological responses against 48‐hr Aeromonas hydrophila exposure challenges of juvenile (4.13 ± 0.12 g) crayfish. The highest values of SGR (2.19% body weight day−1), VFI (2.75% body weight day−1), survival rate (96.67%) and the lowest FCR (2.33) were observed in the juvenile crayfish fed the EGOS‐ diet. The significantly (p < .05) highest means of in vivo ADCOM, in vivo ADCCP, in vivo ADCCF and in vivo ADCGE were measured in crayfish fed the EGOS‐ diet. The mean survival rate of Aeromonas hydrophila‐injected crayfish fed the EGOS‐ diet (56%) was significantly (p < .05) higher than those of fed the control (8.67%) and other diets (22.67–35.32%). At the levels tested, 7.86 log CFU E. faecalis g−1 + 10 g kg−1 GOS in the diet was considered optimum. [ABSTRACT FROM AUTHOR]

A 18-week feeding trial was carried out under controlled conditions to compare the effects of onion powder ( OP) at six levels (5, 10, 20, 30, 40 and 50 g/kg) on the growth performance, nutritional efficiency indices, hemolymph indices and fillet organoleptic properties of juvenile (5.62 ± 0.39 g) crayfish. The significantly ( p < .05) highest values of final weight (71.30 g) and SGR (2.02% per day) and the lowest FCR (1.03) were observed in the juvenile crayfish fed the diet containing 40 g OP/kg. The juvenile crayfish fed the diet containing 40 g OP/kg had the significantly ( p < .05) highest THC (105.27 × 105 cell/ml), HC (98.33 × 105 cell/ml), SGC (38.54 × 105 cell/ml) and LGC (49.51 × 105 cell/ml). The crayfish fed the levels of dietary OP higher than 30 g/kg showed the significantly ( p < .05) higher values of SOD (4.07-4.30 U/min) and LYZ (6.73-7.20 U/min) compared with those fed 5, 10, 20 and 30 g of dietary OP/kg and control. Polynomial regression of SGR, FCR, PPV and PER suggested that the optimum dietary OP level could be higher than 30 and <50 mg/kg in crayfish reared in culture conditions. [ABSTRACT FROM AUTHOR]

La thérapie familiale psychanalytique et l'observation du nourrisson selon la méthode Esther Bick se distinguent a priori dans leurs visées. L'une est un dispositif psychothérapeutique tandis que l'autre se présente comme une formation destinée aux psychothérapeutes. Ces deux démarches ont pour socle commun la théorie psychanalytique, son épistémologie et son éthique. Les cliniciens se laissent guider par les familles qu'ils rencontrent, qu'elles souffrent ou qu'elles aillent « normalement bien ». Penser conjointement ces deux dispositifs permet de dépasser le débat qui oppose le « bébé réel », celui de l'observation directe et développementale, et le « bébé vrai », reconstruit sur le divan. En effet, l'observateur en formation assiste à des mises en récit où ne manquent pas de surgir des éléments fantasmatiques et transgénérationnels, tissant un berceau psychique qui accueille le « bébé réel » et où s'amorce un travail de construction du « bébé vrai ». Les thérapeutes familiaux sont naturellement attentifs à ces constructions et à ces imaginaires pluriels, et aux revendications de ces éternels bébés que les familles portent encore en eux. Ces deux démarches impliquent une attention portée à l'archaïque, aux vécus affectifs et sensoriels et s'appuient l'une et l'autre sur le groupe : l'observateur assiste à la naissance ou au remodelage du groupe familial tandis que le groupe d'appui participe à la transformation du matériel observé en quête d'élaboration. De la même façon, le néo-groupe, composé des thérapeutes familiaux et des familles, soutient un espace de rêverie contenant, propre à faciliter le rétablissement d'une nouvelle forme de circulation fantasmatique. L'observateur, par sa présence, modifie le système familial dans lequel il se trouve de fait inclus, des familles témoignent du profit qu'elles tirent de ces rencontres, et des applications thérapeutiques à domicile ont ainsi été mises en place pour soutenir des familles en difficulté, alors que parallèlement, des praticiens défendent l'intérêt d'un travail familial psychanalytique en périnatalité. L'articulation de ces deux dispositifs nous laisse entrevoir de fécondes perspectives de formation, de prévention et d'accompagnement psychothérapique. Psychoanalytic family therapy and infant observation according to the Esther Bick method differ a priori in their aims. One is a psychotherapeutic device while the other is a training course for psychotherapists. Both approaches have psychoanalytic theory, its epistemology and ethics, as their common basis. The clinicians are guided by the families they meet, whether they are suffering or "normally well". Thinking jointly about these two systems makes it possible to overcome the debate that opposes the "real baby", of direct and developmental observation, and the "true baby", reconstructed on the couch. In fact, the observer in training is witness to the narration of fantasmatic and transgenerational elements, weaving a psychic cradle that welcomes the "real baby" and where the construction of the "true baby" begins. Family therapists are naturally attentive to these plural constructions and imaginaries, and to the claims of these eternal babies that families still carry within them. Both approaches involve attention to the archaic, to affective and sensory experiences, and both rely on the group: the observer witnesses the birth or remodeling of the family group, while the support group participates in the transformation of the observed material in search of elaboration. In the same way, the neo-group, composed of the family therapists and the families, supports a space of containing reverie, which facilitates the re-establishment of a new form of fantasmatic circulation. The observer, by his presence, modifies the family system in which he is in fact included, families testify to the benefit they derive from these meetings, and therapeutic applications at home have thus been set up to support families in difficulty, while at the same time, practitioners defend the interest of psychoanalytical family work in perinatal care. The articulation of these two systems gives us a glimpse of fruitful perspectives for training, prevention and psychotherapeutic support. [ABSTRACT FROM AUTHOR]

Oestradiol plays crucial roles in the mammalian brain by modulating reproductive behaviour, neural plasticity and pain perception. The cephalopod Octopus vulgaris is considered, along with its relatives, to be the most behaviourally advanced invertebrate, although the neurophysiological basis of its behaviours, including pain perception, remain largely unknown. In the present study, using a combination of molecular and imaging techniques, we found that oestradiol up-regulated O. vulgaris gonadotrophin-releasing hormone (Oct-GnRH) and O. vulgaris oestrogen receptor (Oct-ER) mRNA levels in the olfactory lobes; in turn, Oct-ER mRNA was regulated by NMDA in lobes involved in learning and motor coordination. Fluorescence resonance energy transfer analysis revealed that oestradiol binds Oct-ER causing conformational modifications and nuclear translocation consistent with the classical genomic mechanism of the oestrogen receptor. Moreover, oestradiol triggered a calcium influx and cyclic AMP response element binding protein phosphorylation via membrane receptors, providing evidence for a rapid nongenomic action of oestradiol in O. vulgaris. In the present study, we demonstrate, for the first time, the physiological role of oestradiol in the brain lobes of O. vulgaris involved in reproduction, learning and motor coordination. [ABSTRACT FROM AUTHOR]

Abstract: The aim of this study was to establish baseline ultrasound data concerning the mule conceptus during gestation. Ten multiparous Trotter mares were artificially inseminated with chilled semen from an Amiatino jack donkey. Daily transrectal ultrasonography was carried out from the day of ovulation until Day 50 of gestation to determine the following: first detection of the embryonic vesicle (EV), mobility phase, EV diameter, day of EV fixation, changes in EV shape, date of yolk sac regression and embryo crown-rump length. Monthly ultrasonic assessments from Day 50 of gestation to term were carried out. These assessments included an evaluation of fetal well-being and the growth of the mule conceptus, which were monitored using the following variables: cardiac activity, fetal activity and presentation, fetal fluid echogenicity, combined thickness of the utero-placenta unit and fetal orbital and aortic diameter. Mule EV first detection was observed earlier (37% at Day 8) than that observed in the equine pregnancy. EV diameter at first detection was 4.6 ± 1.1 mm. At Day 10, 75% of EVs were detected. EV fixation occurred on Day 17.1 ± 1.1, with a mean EV diameter of 2.5 ± 0.2 cm. EV growth rate was 4.04 mm/day from Days 11 to 16, 0.4 mm/day from Days 16 to 28 and 1.78 mm/day from Days 28 to 45 of pregnancy. The embryo proper was first detected on Day 19.9 ± 1.9 (average length 2.4 ± 1.4 mm), and the embryonic heartbeat was first detected on Day 24 ± 2.4. The fetal carotid pulse was observed at six months of gestation and provided a good means by which to estimate fetal cardiac activity in advanced gestation. The fetal heart rate was recorded from Month 2 of gestation to term. The mean ± SD of the combined uteroplacental thickness was assessed at the cervical-placental junction and at the ventral abdomen in mares between Months 2 and 5 until term, respectively. An abnormal fetal-placental unit and fetal inactivity was observed in association with abortion. Mule-conceptus biometric measurements correlated significantly with the gestational age, and these data were used to predict an unusually large mule fetus, which might result in dystocia. In conclusion, we can assume that early diagnosis of pregnancy failure and assessment of fetal biophysical profile and growth charts could improve the chances of gestation completion in mule-pregnant mares. The early detection of mares at risk for an abnormal pregnancy or delivery may increase the success of prompt treatments, therefore preventing costly emergency procedures and allowing proper obstetrical and neonatal assistance. [Copyright &y& Elsevier]

Abstract: The Interventional Radiology (IR) is a well established technique in medical domain aiming to obtain a good X-ray image quality while minimizing the radiation dose absorbed by patients and staff members. From a radioprotection point of view, these procedures are considered potentially harmful for interventional radiologists and medical staff. Individual monitoring of operators is very important and is performed by assessing effective dose, equivalent dose to the extremities and eye lens, with the use of passive dosimeters (e.g. TLDs) calibrated in terms of personal dose equivalent Hp (10), Hp (0.07) and Hp (3), respectively. In this work we will present a proposal for an innovative X-ray dosimeter prototype, capable of real-time measurements, packaged in a small form-factor, with wireless communication to be used for individual operators dosimetry during IR procedures. The proposed real-time dosimeter relies on CMOS Active Pixel Sensor, widely used in visible imaging applications. Some preliminary results on the characterization of CMOS active pixel sensors as diffused X-ray detectors during a typical IR session will be presented. [Copyright &y& Elsevier]

Male decapod reproduction involves gametogenesis, spermatozoa packaging in spermatophores, changes in cheliped´s growth, and specific behaviors such as courtship, male-male agoniztic interactions, and territory defense. These reproductive-related morpho-functional processes are supported by feed, one of the higher operative costs in aquaculture. This study aimed to evaluate the reproductive variables during the juvenile-mature transition of the male red claw Cherax quadricarinatus fed with two low-cost diets (T1 and T2). Pre-adult males (∼7 g) were individually distributed in plastic containers and fed a diet with fish silage (T1) (n = 9), a diet without fish silage (T2) (n = 8), and a commercial diet for ornamental fish used as a reference diet (T3) (n = 7). The proximal composition of T1, T2, and T3 was 34 %, 28 %, and 47 % for crude protein; 13 %, 9 %, and 6.5 for lipids; and 34 %, 47 %, and 28 % for carbohydrates, respectively. All males acquired sexual maturity with a body weight between 16 and 21 g. All groups showed similar chelae growth (based on the evaluation of the chelae height, and chelae thickness indexes) and red patch development. The gonadosomatic index was significantly lower in the T2 group with respect to T1. The lowest proportion of mature testicular lobes (filled with spermatids/spermatozoa) was detected in the T2 group (49 %), followed by T1 (59 %) group. There was also a lower protein content (∼ 27 %) in the vasa deferentia of the T2 group. However, all males showed a well-developed reproductive system and the histology of the testes and vasa deferentia showed no evidence of structural differences among groups. Under transmission electron microscopy analysis, both the primary and secondary layers of the spermatophore did not show qualitative differences among groups, including the spermatozoon morphology and acrosome size. This study highlights that males fed low-cost diets (0.56 and 0.65 U$S kg− 1, for T1 and T2 respectively), in particular T1, showed reproductive performances as good as the group fed on the reference diet. This is the first analysis of a pool of morpho-functional and biochemical reproductive variables in red claw males, to evaluate the effects of formulated diets on male sexual maturation. [ABSTRACT FROM AUTHOR]

We describe a women 54-year-old, heavy smoker (20 cigarettes/day) who was admitted to our hospital after emergency department admission because of an acute flaccid tetra paresis. Lumbar puncture revealed albumino-cytologic dissociation with 4, 3 gr/L proteins and no cells in CSF. Electromyography concluded for a demielinating poliradicoloneuropathy. Intravenous immunoglobulins (ivIg) 2 gr/Kg in 5 days produced discrete improvement of weakness. Cerebral and cervical spine MRI showed only a left neurinoma of left acustic nerve. Chest and abdomen CT scan revealed a non-specific lung nodule. She was transferred in a rehabilitation department with a near-complete resolution of strength deficits. Patient came back after one year with a severe strength worsening and wheel-chair bounded (MRC score: 22/60). She started 2 gr/Kg and then subcutaneous Ig 1 mg/Kg/months(0.2 mg/Kg every 5 days). In 6 months patient showed a progressive improvement up to MRCscore 40/60.TC-PET scan showed multiple areas of hypermetabolism in the back bone and in anterior-superior iliac spine. The latter was biopsied showing signet-ring cells. Clinical course of patient quickly worsened with drug-resistant seizures and obstructive hydrocephalus which required neurosurgery. After few weeks she died at home. Before neoplastic radiological evidence, subcutaneous Ig were effective in treating strength deficits of this paraneoplastic CIDP. [ABSTRACT FROM AUTHOR]

In this study we evaluated MALDI-TOF MS and FilmArray methods for the rapid identification of yeast from positive blood cultures. FilmArray correctly identified 20/22 of yeast species, while MALDI-TOF MS identified 9/22. FilmArray is a reliable and rapid identification system for the direct identification of yeasts from positive blood cultures. [ABSTRACT FROM AUTHOR]

Carrageenan-based biocomposites have significant potential to develop packaging films and coatings for shelf-life extension of food products. In this study trout fresh fillets were separated into three groups: uncoated trout fillet (UTF), coated with carrageenan-based materials (coated trout fillet, CTF), and coated with carrageenan-based materials containing 1 g/100 mL essential lemon oil (active coated trout fillet, ACTF). The effect of carrageenan coating enriched with essential lemon oil on the quality of rainbow trout ( Oncorhynchusmykiss ) fillets during refrigerated storage (4 ± 1 °C) over a period of 15 days was evaluated. Trout fillets were analyzed for microbiological (total viable count, Enterobacteriacea counts, lactic acid bacteria, H 2 S-producing bacteria), chemical (TVB-N, moisture, pH), and biochemical (fatty acids content) characteristics. This study demonstrates the effectiveness of an edible active carrageenan coating in preserving fresh trout fillets from lipid oxidation and microbial growth. [ABSTRACT FROM AUTHOR]

Nesfatin-1 has recently been indicated as a pleiotropic molecule that is primarily involved in the metabolic regulation of reproductive functions acting at hypothalamic level. The aim of this study was to explore the local action of nesfatin-1 in swine ovarian follicles. Nucleobindin 2 (NUCB2) was verified using real-time quantitative polymerase chain reaction in swine granulosa cells from different sized follicles and nesfatin-1 was localised by immunohistochemistry in sections of the whole porcine ovary. The effects of different concentrations of nesfatin-1 on cell growth, steroidogenesis and the redox status of granulosa cells were determined in vitro. In addition, the effects of nesfatin-1 were evaluated in an angiogenesis bioassay because vessel growth is essential for ovarian follicle function. Immunohistochemistry revealed intense positivity for nesfatin-1 in swine granulosa cells in follicles at all developmental stages. Expression of the gene encoding the precursor protein NUCB2 was higher in granulosa cells from large rather than from medium and small follicles. Further, nesfatin-1 stimulated cell proliferation and progesterone production and interfered with redox status by modifying nitric oxide production and non-enzyme scavenging activity in granulosa cells from large follicles. Moreover, nesfatin-1 exhibited a stimulatory effect on angiogenesis. This study demonstrates, for the first time, that nesfatin-1 is physiologically present in the swine ovarian follicle, where it may impair granulosa cell functions. Reproduction is conditioned by metabolic factors, only some of which are known. This study explored the role of the novel peptide nesfatin-1 in ovarian function. The peptide was localised in the swine ovary and its functions are described. The results of this study contribute to a better understanding of the intricate metabolic network that affects ovarian function. [ABSTRACT FROM AUTHOR]

This study purpose was to evaluate the in vitro inhibitory properties of Italian acacia honey extracts against pathogenic aquatic oomycete/fungal isolates that cause different diseases in crayfish, resulting in an elevated mortality rate. The antimycotic activity of acacia honey aqueous extracts was evaluated against the strain UEF88662 of Aphanomyces astaci (oomycete) and the strain SMM2 of Fusarium avenaceum (fungus). The extracts preparation was carried out with water by a cheap, not complex and organic solvent‐free procedure, with low environmental impact and the higher possibility of large‐scale reproducibility. The anti‐oomycete and antifungal activities were quantitatively evaluated by growth, survival and sporulation microbiological assays. The extracts displayed a dose‐dependent inhibitory efficacy on oomycete and fungal growth and survival, as well as on the production of oomycete and fungal spores. Supported by future in vivo studies, our results encourage the use of natural extracts like honey as innovative tools to counteract mycotic infections. Significance and Impact of the Study: The continuous spread of aquatic fungal disease as the 'crayfish plague' and the 'burn spot disease' has severe ecological and commercial repercussions. Critical factor to prevent further spread is the availability of effective antifungals possibility derived from local natural resources to use in innovative strategies of control and eradication of these diseases. This study provides relevant information about the in vitro anti‐oomycete and antifungal activity of Italian acacia honey aqueous extracts against two highly infectious and dangerous pathogenic species, Aphanomyces astaci and Fusarium avenaceum, that are responsible for important crayfish diseases. Significance and Impact of the Study: The continuous spread of aquatic fungal disease as the 'crayfish plague' and the 'burn spot disease' has severe ecological and commercial repercussions. Critical factor to prevent further spread is the availability of effective antifungals possibility derived from local natural resources to use in innovative strategies of control and eradication of these diseases. This study provides relevant information about the in vitro anti‐oomycete and antifungal activity of Italian acacia honey aqueous extracts against two highly infectious and dangerous pathogenic species, Aphanomyces astaci and Fusarium avenaceum, that are responsible for important crayfish diseases. [ABSTRACT FROM AUTHOR]

Abstract: A pilot project aimed at testing roe enhancement strategies based on offshore Paracentrotus lividus cultures was conducted off the south‐east coast of Italy (Apulia Region). Adult sea urchins were reared in sea cages located 700 m offshore at a depth of 12 m for 3 months. The animals were fed once a week on two formulated diets, prepared mixing nutrients with agar 20 g/Kg and differing only in terms of the protein source: anchovy flour (Diet A) or krill flour (Diet K). At the end of the rearing trial, the gonad somatic index of sea urchins fed on formulated diets significantly exceeded that of wild sea urchins. Total FAA content in the gonads of wild sea urchins and Diet A‐fed sea urchins was similar, whereas in Diet K‐fed sea urchins it was significantly higher. In terms of fatty acids, the gonads contained SFAs, MUFAs and PUFAs. In visual and sensory assessment of gonads by panel test and electronic nose, the gonads of reared sea urchins were rated as being of better size, while no differences were recorded for coloration, taste and odour. This study shows that under these experimental conditions, commercial‐grade Paracentrotus lividus roe enhancement can be achieved after 3 months in sea cages. [ABSTRACT FROM AUTHOR]

Background: Headache disorders are highly prevalent, and have a substantial and negative impact on health worldwide. They are largely treatable, but differences in structure, objectives, organization and delivery affect the quality of headache care. In order to recognize and remedy deficiencies in care, the Global Campaign against Headache, in collaboration with the European Headache Federation, recently developed a set of quality indicators for headache services. These require further assessment to demonstrate fitness for purpose. This is their first implementation to evaluate quality in headache care as a multicentre national study. Methods: Between September and December 2016, we applied the quality indicators in six Italian specialist headache centres (Bologna, Firenze, Modena, Padova, Roma Campus Bio-Medico and Roma Sapienza). We used five previously developed assessment instruments, translated into Italian according to Lifting The Burden's translation protocol for hybrid documents. We took data from 360 consecutive patients (60 per centre) by questionnaire and from their medical records, and by different questionnaires from their health-care providers (HCPs), including physicians, nurses, psychologists and nursing assistants. Results: The findings, comparable between centres, confirmed the feasibility and practicability of using the quality indicators in Italian specialist headache centres. The questionnaires were easily understood by HCPs and patients, and were not unduly time-consuming. Diagnoses were almost all (> 97%) according to ICHD criteria, and routinely (100%) reviewed during follow-up. Diagnostic diaries were regularly used by 96% of physicians. Referral pathways from primary to specialist care existed in five of the six clinics, as did urgent referral pathways. Instruments to assess disability and quality of life were not used regularly, a deficiency that needs to be addressed. Conclusion: This Italy-wide survey confirmed in six specialist centres that the headache service quality indicators are fit for purpose. By establishing majority practice, identifying commonalities and detecting deficits as a guide to quality improvement, the quality indicators may be used to set benchmarks for quality assessment. The next step is extend use and evaluation of the indicators into non-specialist care. [ABSTRACT FROM AUTHOR]

Pectin is a promising candidate to be employed as binder for manufacturing pellets for crayfish feeding. In this study, the gelling properties of different types of pectin and their effects on Cherax quadricarinatus performances have been analysed. Pectins with different degree of esterification from apple and citrus were screened to select those with the best gelling properties to be employed in pellet manufacturing. Three pectins were selected: low esterification degree apple pectin ( LEAP), low esterification citrus pectin ( LECP) and high esterification degree apple pectin ( HEAP). Scanning electron microscopy analysis, nutrient leaching and water uptake indicated that LEAP showed the best aggregating properties, followed by LECP and HEAP pellets. Cherax quadricarinatus performances were affected by the pectin type, with redclaw fed LEAP pellets showing the highest ( P < 0.05) growth rate and amylase activity ( P < 0.05) in the gastrointestinal tract. Altogether these results indicate that LEAP can be considered an excellent binder for feed pellets due to its gelling properties and its efficacy to improve growth and stimulation of digestive enzyme activity. [ABSTRACT FROM AUTHOR]

Abstract: Interventional Radiology (IR) is a subspecialty of radiology comprehensive of all minimally invasive diagnostic and therapeutic procedures performed using radiological devices to obtain image guidance. The interventional procedures are potentially harmful for interventional radiologists and medical staff due to the X-ray diffusion by the patient's body. The characteristic energy range of the diffused photons spans few tens of keV. In this work we will present a proposal for a new X-ray sensing element in the energy range of interest for IR procedures. The sensing element will then be assembled in a dosimeter prototype, capable of real-time measurement, packaged in a small form-factor, with wireless communication and no external power supply to be used for individual operators dosimetry for IR procedures. For the sensor, which is the heart of the system, we considered three different Active Pixel Sensors (APS). They have shown a good capability as single X-ray photon detectors, up to several tens keV photon energy. Two dosimetric quantities have been considered, the number of detected photons and the measured energy deposition. Both observables have a linear dependence with the dose, as measured by commercial dosimeters. The uncertainties in the measurement are dominated by statistic and can be pushed at for all the sensors under test. [Copyright &y& Elsevier]

The algal polysaccharide agar has long been used as a food binder due to its structure, rheological behaviour, stability and interactions - properties that help to generate firm, round, disk-shaped pellets that may be used in recirculating sea urchin-rearing systems. Three algae-based diets ( Ulva lactuca, Gracilaria gracilis, Cystoseira sp.) containing 3% and 6% agar were tested on the sea urchin Paracentrotus lividus in order to examine the effect of varying percentages of agar on pellet stability in water and sea urchin gonad growth. The kinetics of water absorption and solute leaching of pellets were measured by immersing quadruplicate samples of the pellets in water for 1, 2, 3, 4, 5 and 6 days. Our results show that the pellets had good water stability, were readily consumed by sea urchins and the presence of agar did not hamper sea urchin gonad growth. Animals fed Ulva-containing pellets reached a more advanced gametogenic stage with respect to animals fed Cystoseira- and Gracilaria-containing pellets. Moreover, the presence of agarase activity in the digestive system indicated that agar may be an energy source. Pellets are relatively low cost and easy to prepare and store. They may represent a useful resource for rearing sea urchins under intensive conditions. [ABSTRACT FROM AUTHOR]

Abstract: In this study, we used natural polysaccharides (pectin, alginate and chitosan) as binders to generate pellets for crayfish feeding. Pellets were produced by cold extrusion in order to preserve nutrients from degradation and reduce energy consumption. Thereafter, pellets were submitted to a coating procedure, with the aim of improving pellet stability in water. Pellet water stability was analyzed by monitoring the diameter of the released particles in water over progressive time intervals up to 24h, employing a Low Angle Laser Light Scattering Technique. Alginate containing pellets released particles with a smaller diameter than chitosan and pectin containing pellets, indicating that alginate containing pellets disaggregated more and therefore were less stable in water than the other pellet types. The effects of the different polysaccharide containing pellets were evaluated on crayfish feeding response employing juvenile Cherax albidus. The feeding experiment was carried out for 12weeks, at the end of which growth parameters and the activity of amylase, lipases and proteases in the gastric juice, hepatopancreas and intestine were recorded. Crayfish fed pectin containing pellets exhibited a significant weight gain. Digestive enzyme activities did not statistically show significant differences in the digestive tract except for amylase that was significantly higher in the intestine of animals fed pectin containing pellets. Our data indicate that pectin and chitosan pellets showed the best water stability performances, moreover pectin pellets brought about the highest body weight gain and affected the amylase profile in the intestine of juvenile Cherax albidus. [Copyright &y& Elsevier]

In this study we have investigated the role of 17β-estradiol and progesterone in the reproduction of the crayfish Cherax albidus by using vitellogenin (VTG) as a biomarker. Early-vitellogenic (EV), full-vitellogenic (FV), and non-vitellogenic (NV) females of Cherax albidus were treated with 17β-estradiol, progesterone, or both for 4 weeks. Levels of VTG mRNA in the hepatopancreas were detected by RT-PCR. The PCR product was sequenced and showed 97% homology with Cherax quadricarinatus VTG. 17β-estradiol was more effective than progesterone and 17β-estradiol plus progesterone in increasing the vitellogenm transcript in the hepatopancreas of EV and FV females. On the contrary, progesterone was more effective than 17β-estradiol and 17β-estradiol plus progesterone in increasing the vitellogenin concentration in the hemolymph of EV and FV females. Hepatopancreas histology and fatty acid composition of females injected with hormones showed major modifications. No effects were registered in NV females. In conclusion, 17β-estradiol and progesterone influence VTG synthesis, although our data indicate that they act through different pathways and are not effective until the proper hormonal environment is established, as demonstrated by their inefficacy in NV females. [ABSTRACT FROM AUTHOR]

Human malignant pleural mesothelioma (MPM) is a dreadful disease and there is still no standard therapy available for a consistent therapeutic approach. This research is aimed at the evaluation of the potential therapeutic effect of a specific nicotinic receptor (nAChR) antagonist, namely alpha-Cobratoxin (alpha-CbT). Its effectiveness was tested in mesothelioma cell lines and in primary mesothelioma cells in vitro, as well as in vivo, in orthotopically xenotransplanted NOD/SCID mice. Cells showed alpha7-nAChR expression and their growth was significantly inhibited by alpha-CbT. Severe induction of apoptosis was observed after exposure to alpha-CbT [IC80-90]. Apoptosis was characterised by: change in mitochondrial potential, caspase-3 cleavage, down-regulation of mRNA and protein for survivin, XIAP, IAP1, IAP2 and Bcl-XL, inhibition by caspase-3 inhibitor. In vivo, the alpha-CbT acute LD50 was 0.15 mg/kg. The LD100 [0.24 mg/kg] induced fatal respiratory failure and massive kidney necrosis. Phase II experiments with 0.12 ng/kg alpha-CbT (1/1000 of LD10) were done in 53 xenotransplanted mice, inhibiting tumour development as confirmed by chest X-ray examinations, autopsy and microscopical findings. The growth of human proliferating T lymphocytes and of mesothelial cells in primary culture was not affected by alpha-CbT. Non-immunogenic derivatives of the alpha-CbT molecule need to be developed for possible human use. [ABSTRACT FROM AUTHOR]

Abstract: Two algal polysaccharides: alginate and agar and one polysaccharide from fruit, pectin, have been used as food additives in relation to their structure and their rheological behavior, stability and interactions, to generate firm pellets to be employed in crayfish aquaculture. Three diets containing the same amount of nutrients (protein, lipid and carbohydrate) mixed with each of the three different polysaccharides were tested on male freshwater crayfish Cherax albidus growth. The aims of this study were to examine the effect of alginate, agar and pectin, on pellet stability in water, and on crayfish growth. The kinetics of water absorption and solute leaching of pellets were measured by immersing quadruplicate samples of the diets in water for 0.5, 2, 4 and 8 h. After the required immersion time, the samples were recovered, freeze-dried for 24 h and re-weighed. Recovered liquid was analyzed by Low Angle Laser Light Scattering Technique, which allowed the monitoring of the released particles in water during immersion. At the same time, the crayfish feeding response to firm pellets was monitored in feeding behavior and growth experiments. Our results show that pellets had good water stability, were preferred by crayfish and the presence of polysaccharides positively affected crayfish growth. Indeed, animals fed pellets showed a higher percentage of growth when compared to animals fed a fresh diet. In particular, pectin gave the best results, showing better water stability than alginate and agar and causing the best growth performance. Pellets are relatively low cost and easy to make and they may represent an advantage for those who want to farm crayfish under intensive conditions. [Copyright &y& Elsevier]

Genetic studies indicate that chromosome 7q is likely to contain an autism susceptibility locus (AUTS1). We have followed a positional candidate gene approach to identify relevant gene(s) and report here the analysis of reelin (RELN), a gene located under our peak of linkage. Screening RELN for DNA changes identified novel missense variants absent in a large control group; however, the low frequency of these mutations does not explain the relatively strong linkage results on 7q. Furthermore, analysis of a previously reported triplet repeat polymorphism and intragenic single nucleotide polymorphisms, using the transmission disequilibrium test, provided no evidence for association with autism in IMGSAC and German singleton families. The analysis of RELN suggests that it probably does not play a major role in autism aetiology, although further analysis of several missense mutations is warranted in additional affected individuals.Molecular Psychiatry (2003) 8, 885-892. doi:10.1038/sj.mp.4001310 [ABSTRACT FROM AUTHOR]

Solid waste, in dealing with the growing presence of organic compounds, especially plastics, can be considered an important source of energy since they are inexpensive and easily obtainable materials. However, the presence of polyvinyl chloride (PVC) in the waste can result in a recycling problem when thermal treatment is involved.This work shows the possibilities of recovering energy from waste containing PVC by a gasification process without additional dechlorination facilities.Experimental data obtained in a bench scale two-stage reactor indicate that the addition of Na2CO3 in a blend containing refuse-derived fuel (RDF) and PVC is efficient in eliminating chlorine from the produced off-gas. On the contrary, the less expensive calcium compounds do not show a satisfactory chlorine removal. A thermodynamic simulation of the process substantially leads to the same results.Starting from this study, the working conditions for gasifying waste blends containing PVC in a scaled-up two-stage reactor can be predicted. According to the simulation, the obtained syngas shows that the polluting compounds content is lower than the Italian law limit. As a consequence, it can be used directly to obtain electric power and/or local heating. [Copyright &y& Elsevier]

We evaluated in vitro the toxicity of idarubicin and its active metabolite idarubicinol on haematopoietic progenitors, using human umbilical cord blood and peripheral blood progenitors to obtain dose -- response curves. We treated 16 patients with poor prognosis lymphoma in a phase I-II trial of high-dose idarubicin and melphalan and investigated if idarubicinol persisting in patients' plasma at the time of transplantation (day 0), on day +1 and +2 could result in an inhibition of infused progenitors. Colony inhibition was correlated with pharmacokinetic data and with the time of patients' engraftment. Plasma samples obtained before idarubicin treatment demonstrated a colony-stimulating effect, increasing the cloning efficiency by 72%. The inhibitory activity on colony forming unit granulocyte -- macrophage (CFU-GM) of patients' plasma collected on the day of transplantation was lower than expected from dose -- response curves (21% measured vs 70% expected). The time to patients' WBC and PLT recovery correlated with the amount of CD34+ cells reinfused and, to a lesser extent, with the colony-inhibiting effect of patients' plasma. The correlation between idarubicinol concentration and CFU-GM inhibition was not significant. These data suggest that plasma drug concentration on the day of stem cell reinfusion may overestimate the toxicity of residual anthracyclines to the transplanted cells. [ABSTRACT FROM AUTHOR]

Medical procedures where real-time X-ray imaging is needed have rapidly increased in number in the past decades. Consequently, the personnel dosimetry for medical staff has become more important, also in light of the revision of worker safety regulations. Detailed knowledge of the dose-rate is needed to reduce the total absorbed dose during a single procedure. Real-time monitoring through wearable active devices would greatly facilitate this task. In this work, we will present the characterization of the RAPID wireless device (based on CMOS imager as X-ray sensing detector) in clinical conditions, with PMMA phantoms. The prototype has shown the capability to measure dose-rates with a frequency in the range of few Hz, and an uncertainty smaller than 10%. [ABSTRACT FROM AUTHOR]

An abstract of the study "Responsiveness to Cardiac Resynchronization Therapy (CRT) in Ischemic vs. Non-Ischemic Patients With Heart Failure (HF) and Left Bundle Branch Block," by F. Oliva and colleagues is presented.