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This is the text, largely unedited, of a speech that I gave at the funeral of my aunt Anne Turvey earlier this year. In it, to borrow a phrase of my aunt's of which I have recently been reminded, I tried to 'go to work' on the double-ness that I experienced as I got to know my aunt as a professional adult when I arrived at the Institute of Education in 2018. By that time, although we did not know it then, my aunt was likely already suffering from the disease that would eventually take her from us. I try, here, to preserve and present some part of what it meant to know Annie, Anne, and them both together. If you had the pleasure of knowing Annie/Anne too, you'll know that this only scratches the surface of who she was. If not, allow me to introduce my aunt. [ABSTRACT FROM AUTHOR]

Migration pathways and distances of the oils in reservoir are thought to affect the distribution of pyrrolic compounds such as carbazole, its alkyl derivatives (alkylated carbazoles) and benzocarbazoles, although other factors, including maturity and depositional environments may also affect the distribution of these organic nitrogen compounds. In this study, 14 oil samples produced from conventional reservoirs in Pauls Valley, south Oklahoma were investigated using organic geochemical techniques. The sterane and hopane fingerprints suggest that most of the oils were sourced from the Devonian Woodford shale. Maturity parameters consistently indicate that the maturity level of the studied samples are all of similar maturity (Rc = ∼0.7%), suggesting the distribution of the organic nitrogen organic compounds is possibly reflecting variations in relative migration distances. The distribution of alkylcarbazoles revealed a preferential enrichment during migration, with the nitrogen-shielded alkylcarbazole tending to be enriched relative to the nitrogen-semi-shielded alkylcarbozoles particularly in oils produced close to the Arbuckle uplift to the east. Correspondingly, another family of pyrrolic compounds, benzocarbazoles, whose distributions also indicated that the Pauls Valley Woodford oils came from deeper part of the Anadarko Basin as the benzo-[a]/([a]+[c])-carbazole ratios decrease eastwards. In more specific migration systems, although the pyrrolic compound indicators are potentially disturbed by the structural complexes, the general migration directions suggest that the studied oils in Pauls Valley Hunton uplift were sourced from the deep basin area, and migrate upwards in porous sediments due to the buoyancy. This study investigates the feasibility of using pyrrolic compounds to estimate relative migration distances and will aid in the interpretation of migration history by using the distribution of carbazole, alkylated carbazoles, benzocarbazoles isomers in the Anadarko and Ardmore Basin petroleum systems. [ABSTRACT FROM AUTHOR]

Introduction: Many stroke survivors do not receive optimal levels of personalised therapy to support their recovery. Use of technology for stroke rehabilitation has increased in recent years to help minimise gaps in service provision. Markerless motion capture technology is currently being used for musculoskeletal and occupational health screening and could offer a means to provide personalised guidance to stroke survivors struggling to access rehabilitation. Aims: This study considered context, stakeholders, and key uncertainties surrounding the use of markerless motion capture technology in community stroke rehabilitation from the perspectives of stroke survivors and physiotherapists with a view to adapting an existing intervention in a new context. Methods: Three focus groups were conducted with eight stroke survivors and five therapists. Data were analysed using reflexive thematic analysis. Results: Five themes were identified: limited access to community care; personal motivation; pandemic changed rehabilitation practice; perceptions of technology; and role of markerless technology for providing feedback. Conclusions: Participants identified problems associated with the access of community stroke rehabilitation, exacerbated by Covid-19 restrictions. Participants were positive about the potential for the use of markerless motion capture technology to support personalised, effective stroke rehabilitation in the future, providing it is developed to meet stroke survivor specific needs. [ABSTRACT FROM AUTHOR]

Revealing unknown cues that regulate oligodendrocyte progenitor cell (OPC) function in remyelination is important to optimise the development of regenerative therapies for multiple sclerosis (MS). Platelets are present in chronic non-remyelinated lesions of MS and an increase in circulating platelets has been described in experimental autoimmune encephalomyelitis (EAE) mice, an animal model for MS. However, the contribution of platelets to remyelination remains unexplored. Here we show platelet aggregation in proximity to OPCs in areas of experimental demyelination. Partial depletion of circulating platelets impaired OPC differentiation and remyelination, without altering blood-brain barrier stability and neuroinflammation. Transient exposure to platelets enhanced OPC differentiation in vitro, whereas sustained exposure suppressed this effect. In a mouse model of thrombocytosis (Calr+/-), there was a sustained increase in platelet aggregation together with a reduction of newly-generated oligodendrocytes following toxin-induced demyelination. These findings reveal a complex bimodal contribution of platelet to remyelination and provide insights into remyelination failure in MS. [ABSTRACT FROM AUTHOR]

This Meta-Aggregative (MA) Qualitative Evidence Synthesis (QES) identified, investigated, and critically appraised examples of 'quality' Teacher Critical Reflection (TCR) in recent peer-reviewed research. The key question guiding the synthesis of literature was, How is teacher critical reflection evidenced, justified, and communicated in peer-reviewed research? The synthesis detected the importance of TCR in supporting quality teacher practice in early childhood and primary school settings. The MA element guided the choice of articles and the QES identified evidence of the intricacies of teacher critical reflection. The MA QES provides insights into sustainable and purposeful TCR. Thirteen studies conducted between 2013 and 2019 met the requirements of a four-phased MA QES and were critically appraised to inform the synthesis. Recommendations derived from this review include the importance of identifying the objectives of TCR, TCR as part of teacher identity and its impact on TCR, the significant role that social collaboration plays in TCR, and the tools that support successful TCR. [ABSTRACT FROM AUTHOR]

What are the benefits of liberating museum objects from their colonial frames of reference to reincorporate them into Indigenous ways of knowing? As curators working together with Torres Strait Islanders to interpret museum objects, the authors focus on collected items relating to centipedes acquired during the experimental era of scientific investigation by a zoologist and an ethnographer in eastern Zenadth Kes, the Torres Strait waters between northern Australia and Papua New Guinea. In the scientific sphere, the centipede Scolopendra existed for a time as an insect before "becoming" a myriapod. In Zenadth Kes it has always existed as isi. [ABSTRACT FROM AUTHOR]

While the family unit in the Gothic novel has been widely discussed, the figure of the Gothic cousin has largely been relegated to the periphery of critical scholarship. This paper contends that the cousin occupies a particularly unheimlich position in the family unit: a cousin might be of the same age, or so distant in age that they are almost a stranger; they might be entirely unfamiliar, or raised from childhood with their cousins; they might be an acceptable romantic interest or an entirely taboo one. While clearly a flexible, shifting figure within the family unit, in many Gothic novels the arrival of a cousin causes significant upheaval – either immediate or generational – that undermines, dismantles, or enacts a renegotiation of the domestic order. The intrusions of Charles in Shirley Jackson's We Have Always Lived in the Castle and Rachel in Daphne du Maurier's My Cousin Rachel both reflect and reinforce this trajectory. In this paper, we consider the ways in which Rachel and Charles support our interpretation of the cousin as a disruptive figure within Gothic texts. [ABSTRACT FROM AUTHOR]

At a time when the scope of One Health is expanding, the term "biomanufacturing" has taken on new significance as a new route to more sustainable manufacturing in the face of the current overwhelming reliance on fossil resources for fuel, energy, and materials. This article looks at how One Health and bio - manufacturing interact from policy, technical, and societal viewpoints. The biofoundry is explored as a missing link in the design phase of biomanufacturing and examples are given where the potential of biofoundries can be enhanced in selected environmental and human health applications. [ABSTRACT FROM AUTHOR]

Youth participation in agricultural entrepreneurship (agripreneurship) is vital to the sustainable and inclusive modernisation of agrarian societies. We analysed relationships between demographic factors and motivational antecedents of intention to practise small and medium-scale agripreneurship using survey data collected from undergraduate students in the Lao People's Democratic Republic. Gender significantly influenced attitudes, family occupational background influenced subjective norms, and place of origin influenced perceived behavioural control: three constructs hypothesised to drive intention according to the theory of planned behaviour. Although students with commercial agriculture/agribusiness family occupational backgrounds were found to have a greater intention to practise agripreneurship than students without, students with subsistence farming family occupational backgrounds were not. Commercialisation of agriculture in Laos therefore requires better engagement of youth from subsistence farming households, as this group has the most to contribute and is at the greatest risk of missing the benefits of modernisation. [ABSTRACT FROM AUTHOR]

Angiotensin-converting enzyme 2 (ACE2) is not only the viral receptor for the novel coronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) but is also classically known as a key carboxypeptidase, which through multiple interacting partners plays vital physiological roles in the heart, kidney, lung, and gastrointestinal tract. An accumulating body of evidence has implicated the dysregulation of ACE2 abundance and activity in the pathophysiology of multiple disease states. ACE2 has recently regained attention due to its evolving role in driving the susceptibility and disease severity of coronavirus disease 2019 (COVID-19). This narrative review outlines the current knowledge of the structure and tissue distribution of ACE2, its role in mediating SARS-CoV-2 cellular entry, its interacting partners, and functions. It also highlights how SARS-CoV-2-mediated dysregulation of membrane-bound and circulating soluble ACE2 during infection plays an important role in the pathogenesis of COVID-19. We explore contemporary evidence for the dysregulation of ACE2 in populations that have emerged as most vulnerable to COVID-19 morbidity and mortality, including the elderly, men, and pregnant women, and draw attention to ACE2 dynamics and discrepancies across the mRNA, protein (membrane-bound and circulating), and activity levels. This review highlights the need for improved understanding of the basic biology of ACE2 in populations vulnerable to COVID-19 to best ensure their clinical management and the appropriate prescription of targeted therapeutics. [ABSTRACT FROM AUTHOR]

Airway remodeling occurs in chronic asthma leading to increased airway smooth muscle (ASM) mass and extracellular matrix (ECM) deposition. Although extensively studied in murine airways, studies report only selected larger airways at one time-point meaning the spatial distribution and resolution of remodeling are poorly understood. Here we use a new method allowing comprehensive assessment of the spatial and temporal changes in ASM, ECM, and epithelium in large numbers of murine airways after allergen challenge. Using image processing to analyze 20-50 airways per mouse from a whole lung section revealed increases in ASM and ECM after allergen challenge were greater in small and large rather than intermediate airways. ASM predominantly accumulated adjacent to the basement membrane, whereas ECM was distributed across the airway wall. Epithelial hyperplasia was most marked in small and intermediate airways. After challenge, ASM changes resolved over 7 days, whereas ECM and epithelial changes persisted. The new method suggests large and small airways remodel differently, and the long-term consequences of airway inflammation may depend more on ECM and epithelial changes than ASM. The improved quantity and quality of unbiased data provided by the method reveals important spatial differences in remodeling and could set new analysis standards for murine asthma models. [ABSTRACT FROM AUTHOR]

Many biotechnology applications tend to be for low production volumes and relatively high-value products such as insulin and vaccines. More difficult to perfect at scale are bioprocesses for high-volume products with lower value, especially if the target product is a reduced chemical such as a solvent or a plastic. Historically, industrial microbiology succeeded under special circumstances when fossil feedstocks were either unavailable or expensive. Inevitably, as these circumstances relaxed, bioprocesses struggled to compete with petrochemistry. Why try to compete? Fossil resources will be phased out in the coming decades in the struggle with climate change. To reach net-zero carbon by 2050 will require all sectors to transition, not only energy and transportation. This may herald a new opportunity for industrial bioprocesses with much better tools. Some of the issues around biotechnology in the first issue of Trends in Biotechnology remain issues today despite huge advances in technology. The Weizmann process and single-cell protein thrived, died, but may be on their way back. The bioeconomy envisages the gradual replacement of oil and gas, but the price of oil is very often a determining factor in the success of any competing technology. Net-zero carbon aspirations should be a huge opportunity for biotechnologies. The automation possible in the biofoundry allied to automated chemical synthesis seems to hold the key. Many of the hardest-to-abate industrial sectors cannot be decarbonized, but they might be 'defossilized'. Governments need to invest in bio-based manufacturing to bring them more quickly to the marketplace. [ABSTRACT FROM AUTHOR]

Nicotinamide adenine dinucleotide (NAD+) is a vital energy intermediate in skeletal muscle. The discovery of dietary-derived NAD+ precursors has led to the rapid development of NAD+ therapeutics designed to manipulate NAD+ content in target tissues. Of those developed, nicotinamide riboside and nicotinamide mononucleotide have been reported to display health benefit in humans under clinical scenarios of NAD+ deficiency. In contrast, relatively little is known regarding the potential benefit of nicotinamide riboside and nicotinamide mononucleotide supplementation in healthy individuals, with questions remaining as to whether NAD+ therapeutics can be used to support training adaptation or improve performance in athletic populations. Examining animal and human nicotinamide riboside supplementation studies, this review discusses current evidence suggesting that NAD+ therapeutics do not alter skeletal muscle metabolism or improve athletic performance in healthy humans. Further, we will highlight potential reasons why nicotinamide riboside supplementation studies do not translate to healthy populations and discuss the futility of testing NAD+ therapeutics outside of the clinical populations where NAD+ deficiency is present. [ABSTRACT FROM AUTHOR]

Mitochondria are dynamic organelles, intricately designed to meet cellular energy requirements. To accommodate alterations in energy demand, mitochondria have a high degree of plasticity, changing in response to transient activation of numerous stress‐related pathways. This adaptive response is particularly relevant in highly metabolic tissues such as skeletal muscle, where mitochondria support numerous biological processes related to metabolism, growth and regeneration. Aerobic exercise is a potent stimulus for skeletal muscle remodelling, leading to alterations in substrate utilisation, fibre‐type composition and performance. Underlying these physiological responses is a change in mitochondrial quality control (MQC), a term encompassing the co‐ordination of mitochondrial synthesis (biogenesis), remodelling (dynamics) and degradation (mitophagy) pathways. Understanding of MQC in skeletal muscle and the regulatory role of aerobic exercise of this process are rapidly advancing, as are the molecular techniques allowing the study of MQC in vivo. Given the emerging link between MQC and the onset of numerous non‐communicable diseases, understanding the molecular regulation of MQC, and the role of aerobic exercise in this process, will have substantial future impact on therapeutic approaches to manipulate MQC and maintain mitochondrial function across health span. [ABSTRACT FROM AUTHOR]

Objective: To describe the clinical and clinicopathological characteristics, treatment, and outcome for dogs and cats with a confirmed foxtail. Design: Retrospective study utilizing a medical records database over a 10‐year period from January 1, 2009 to December 31, 2018. Setting: University teaching hospital. Animals: Seven hundred and fifty‐four dogs and 37 cats with a diagnosis of foxtail foreign body. Cases with direct visualization by a clinician or on histopathology were included. Information extracted for each case included signalment; anatomical foxtail location; clinicopathological and imaging findings; treatments and interventions provided; and outcome. Measurements and Main Results: The prevalence of foxtail associated disease was 0.25% in dogs and 0.07% in cats over this time period. Most animals were young to middle‐aged and presented in the summer months. The most common location in dogs was the aural canal, cutaneous/subcutaneous space, and nasal canal. In cats, ocular foxtails were most common (30/37). Blood work changes were nonspecific. Ultrasound supervised by a boarded radiologist was utilized in 114 cases, mainly for subcutaneous, sublumbar, and intracavitary foxtail locations, with successful location of a foxtail in 72.8% of cases scanned. Computed tomography was performed in 78 dogs with suspected intracavitary foxtail migration, and in all cases, structural changes related to the presence of the foxtail were found. Anerobic bacteria were most commonly isolated when a culture was submitted, with Actinomyces spp. rarely isolated. The most common of the 120 anaerobic isolates were Bacteroides/Prevotella spp. (n = 38), Fusobacterium spp. (n = 32), and Peptostreptococcus anaerobius (n = 30). Conclusions: The short‐term outcome for foxtail‐associated lesions is good, and most cases can be managed on an outpatient basis. A minority of cases develop life‐threatening disease and may require a multidisciplinary approach of multimodal imaging, endoscopy, or surgery. [ABSTRACT FROM AUTHOR]

Afterschool programs are increasingly recognized as valuable opportunities for learning that can create social capital, expose students to topics of interest, and build personal identities. Yet poor policies and ineffective practices driven by outdated ideals abound, including an emphasis on the caregiving function of afterschool programs for working families, as well as a focus on academic skill-building and remediation, often to the detriment of adolescents and students of color. I review historical trends and recent controversies alongside empirical and theoretical research to demonstrate the importance of afterschool for learning and development, particularly for students who are most likely to disengage from traditional school cultures. Using learning ecosystems models, I offer insights into how education leaders can leverage the strengths of afterschool programs to support academic achievement without simply extending the school day into afterschool. [ABSTRACT FROM AUTHOR]

This study investigated whether heat acclimation (HA) could improve rowing performance in temperate conditions in national-level rowers. Using a parallel-group design, eleven rowers (3 female, 8 male, age: 21±3 years, height: 182.3±6.8cm, mass: 79.2±9.0kg, V˙O2peak : 61.4±5.1ml·kg·min-1) completed either a HA intervention (HEAT, n = 5) or acted as controls (CON, n = 6). The intervention replaced usual cross-training sessions and consisted of an hour of submaximal cycling or rowing ergometry in either 34±0°C for HEAT or 14±1°C for CON daily over two five-day blocks (10 sessions total), separated by 72h. Participants performed the '10+4' test that consists of 10-min submaximal rowing and a 4-min time-trial (TT) in temperate conditions (20±0°C) before and after the intervention. Heat acclimation following the 10-session intervention was evidenced by large significant (p<0.05) decreases in maximum tympanic temperature (d = -1.68) and rate of perceived exertion (RPE) (d = -2.26), and a large significant increase in sweat loss (d = 0.91). Large non-significant (p>0.05) decreases were seen in average tympanic temperature (d = -3.08) and average heart rate (d = -1.53) in HEAT from session 2 to session 10 of the intervention. Furthermore, a large significant increase was seen in plasma volume (d = 3.74), with large significant decreases in haemoglobin concentration (d = -1.78) and hematocrit (d = -12.9). Following the intervention, large non-significant increases in respiratory exchange ratio (d = 0.87) and blood lactate (d = 1.40) as well as a large non-significant decrease in RPE (d = -1.23) were seen in HEAT during the 10-min submaximal rowing. A large significant decrease in peak heart rate (d = -2.27), as well as a large non-significant decrease in relative V˙O2peak (d = -0.90) and large non-significant increases in respiratory exchange ratio (d = 1.18), blood lactate concentration (d = 1.25) and power output (d = 0.96) were seen in HEAT during the 4-min TT. This study suggests that a 10-session HA intervention may elicit HA in national-level rowers, with potential to improve 4-min TT performance in temperate conditions. [ABSTRACT FROM AUTHOR]

Buxton, JD, Prins, PJ, Miller, MG, Moreno, A, Welton, GL, Atwell, AD, Talampas, TR, and Elsey, GE. The effects of a novel quadrupedal movement training program on functional movement, range of motion, muscular strength, and endurance. J Strength Cond Res 36(8): 2186–2193, 2022—Quadrupedal movement training (QMT) is a form of bodyweight training incorporating animal poses, transitions, and crawling patterns to reportedly improve fitness. This type of training may improve multiple facets of fitness, unfortunately, little evidence exists to support commercial claims and guide practitioners in the best use of QMT. Therefore, the purpose of this study was to assess the impact of a commercially available QMT program on functional movement, dynamic balance, range of motion, and upper body strength and endurance. Forty-two active college-age (19.76 ± 2.10 years) subjects (males = 19, females = 23) were randomly assigned to a QMT (n = 21) or control (CON) (n = 21) group for 8 weeks. Quadrupedal movement training consisted of 60-minute classes performed 2×·wk−1 in addition to regular physical activity. Active range of motion, Functional Movement Screen (FMS), Y-Balance Test (YBT), handgrip strength, and push-up endurance were assessed before and after the intervention. The QMT group showed significantly greater improvements than the CON group in FMS composite score (1.62 ± 1.53 vs. 0.33 ± 1.15, p = 0.004) and FMS advanced movements (0.81 ± 0.87 vs. 0.01 ± 0.71, p = 0.002) and fundamental stability (0.57 ± 0.75 vs. 0.05 ± 0.50, p = 0.011), along with hip flexion, hip lateral rotation, and shoulder extension (p < 0.05). No significant differences between groups were observed for dynamic balance or upper body strength and endurance. Our results indicate that QMT can improve FMS scores and various active joint ranges of motion. Quadrupedal movement training is a viable alternative form of training to improve whole-body stabilization and flexibility. [ABSTRACT FROM AUTHOR]

Ion channels are potentially exploitable as pharmacological targets to treat asthma. This study evaluated the role of KCa3.1 channels, encoded by Kcnn4, in regulating the gene expression of mouse airway epithelium and the development of asthma traits. We used the ovalbumin (OVA) challenge as an asthma model in wild-type and Kcnn4-/- mice, performed histological analysis, and measured serum IgE to evaluate asthma traits. We analyzed gene expression of isolated epithelial cells of trachea or bronchi using mRNA sequencing and gene ontology and performed Ussing chamber experiments in mouse trachea to evaluate anion secretion. Gene expression of epithelial cells from mouse airways differed between trachea and bronchi, indicating regional differences in the inflammatory and transepithelial transport properties of proximal and distal airways. We found that Kcnn4 silencing reduced mast cell numbers, mucus, and collagen in the airways, and reduced the amount of epithelial anion secretion in the OVA-challenged animals. In addition, gene expression was differentially modified in the trachea and bronchi, with Kcnn4 genetic silencing significantly altering the expression of genes involved in the TNF pathway, supporting the potential of KCa3.1 as a therapeutic target for asthma. [ABSTRACT FROM AUTHOR]

Background: Upper-limb movement analysis could improve our understanding of function, pathological mechanisms and inform rehabilitation and surgical decision-making. Despite the potential benefits, the use of clinical upper-limb motion analysis is not well established and it is not clear what the barriers to clinical motion analysis are.Research Question: What is current practice for assessment of the upper-limb and what are the barriers currently limiting upper-limb motion analysis being routinely used in clinical practice?Methods: A web-based questionnaire was used to collect responses through international professional movement analysis society coordinators over an 18 month-period.Results: A total of 55 responses were received and 75% of laboratories performed some form of upper-limb assessment. In total 44% of laboratories performed upper-limb assessments for clinical purposes and only 33% did 3D-movement analysis. The most commonly seen patient groups were those with neurological injury e.g. cerebral palsy (adults and children) and normal controls for comparative purposes. Barriers to upper-limb motion analysis were the availability of standard reference tasks, protocols, software, funding and clinical need. Practice was variable with no universally identified approaches to upper-limb movement analysis. Differences in practice were also identified between laboratories accredited by the Clinical Movement Analysis Society of the UK and Ireland and other international professional societies and affiliate laboratories.Significance: These findings may be used to inform the development of practice standards and progress the use of clinical motion analysis in the upper-limb. This study provides a summary and describes current practice, potentially providing access to peer support and experience for laboratories with an identified clinical need looking to conduct upper-limb assessment. A national picture (UK and Ireland) for practice regarding upper-limb assessment in this sub-population is presented. We have laid out further work which is needed to establish standards of practice or consensus initiatives for enhancing clinical upper-limb motion analysis. [ABSTRACT FROM AUTHOR]

For patients affected by Facioscapulohumeral dystrophy (FSHD), alternate methods for increasing physical activity engagement that may benefit shoulder function and wider health are needed. Arm cycling has been proposed as a potential method for achieving this although dosage parameters and evidence is limited. The aim of this study was to conduct a pilot study evaluating the effect of a single intermittent arm cycling exercise programme on people affected by FSHD. People with confirmed genetic diagnosis of FSHD between the ages 18–60 years were recruited to attend a single session for the exercise intervention (5 exercise efforts lasting 2 minutes each with 30 seconds of rest between each effort). Prior to exercise, measures of shoulder function (Oxford shoulder score), strength and range of movement were recorded. During the exercise participants were video recorded to quantify range of movement and extract movement profile features. Participants comments were recorded and followed up four days later to check for adverse events. Fifteen participants, (6F:9M) were recruited with median (IQR) Oxford Shoulder Scores of 25 (18 to 39). All participants successfully completed the exercise intervention with only transient symptoms consistent with exercise being reported and achieving a median (IQR) rate of perceived exertion scores of 13 (12 to 13). Movement profile data was available for 12 out of 15 participants and suggests that exercise intensity did not compromise movement. An association between strength and shoulder function (R2 = 0.5147), Rate of perceived exertion (RPE) of the final effort against shoulder function and strength (R2 = 0.2344 and 0.1743 respectively) was identified. Participant comments were positive regarding the exercise intervention. Our study demonstrates that an intermittent arm cycling programme is feasible for people affected by FSHD. Further work is needed to evaluate physiological responses to exercise across variations in programme variables and equipment set up in a larger sample of people affected by FSHD. [ABSTRACT FROM AUTHOR]

Anatomy Nights is an international public engagement event created to bring anatomy and anatomists back to public spaces with the goal of increasing the public's understanding of their own anatomy by comparison with non-human tissues. The event consists of a 30-minute mini-lecture on the anatomy of a specific anatomical organ followed by a dissection of animal tissues to demonstrate the same organ anatomy. Before and after the lecture and dissection, participants complete research surveys designed to assess prior knowledge and knowledge gained as a result of participation in the event, respectively. This study reports the results of Anatomy Nights brain events held at four different venues in the UK and USA in 2018 and 2019. Two general questions were asked of the data: 1) Do participant post-event test scores differ from pre-event scores; and 2) Are there differences in participant scores based on location, educational background, and career. We addressed these questions using a combination of generalized linear models (R's glm function; R version 4.1.0 [R Core Team, 2014]) that assumed a binomial distribution and implemented a logit link function, as well as likelihood estimates to compare models. Survey data from 91 participants indicate that scores improve on post-event tests compared to pre-event tests, and these results hold irrespective of location, educational background, and career. In the pre-event tests, participants performed well on naming structures with an English name (frontal lobe and brainstem), and showed signs of improvement on other anatomical names in the post-test. Despite this improvement in knowledge, we found no evidence that participation in Anatomy Nights improved participants' ability to apply this knowledge to neuroanatomical contexts (e.g., stroke). [ABSTRACT FROM AUTHOR]

Burkholderia vietnamiensis B418 is a multifunctional plant growth-promoting rhizobacteria (PGPR) strain with nitrogen-fixing and phosphate-solubilizing capability which can be employed for root-knot nematode (RKN) management on various crops and vegetables. Here we investigated the control efficacy of B. vietnamiensis B418 inoculation against RKN on watermelon, applied either alone or combined with nematicides fosthiazate or avermectin, and their effects on bacterial and fungal microbiomes in rhizosphere soil. The results of field experiments showed individual application of B418 displayed the highest control efficacy against RKN by 71.15%. The combinations with fosthiazate and avermectin exhibited slight incompatibility with lower inhibitory effects of 62.71% and 67.87%, respectively, which were still notably higher than these nematicides applied separately. Analysis of microbiome assemblages revealed B418 inoculation resulted in a slight reduction for bacterial community and a significant increment for fungal community, suggesting that B418 could compete with other bacteria and stimulate fungal diversity in rhizosphere. The relative abundance of Xanthomonadales, Gemmatimonadales and Sphingomonadales increased while that of Actinomycetales reduced with B418 inoculation. The predominate Sordariomycetes of fungal community decreased dramatically in control treatment with B418 inoculation whereas there were increments in fosthiazate and avermectin treatments. Additionally, nitrogen (N) cycling by soil microbes was estimated by quantifying the abundance of microbial functional genes involved in N-transformation processes as B418 has the capability of N-fixation. The copy number of N-fixing gene nifH increased with B418 inoculation, and the highest increment reached 35.66% in control treatment. Our results demonstrate that B. vietnamiensis B418 is an effective biological nematicide for nematode management, which acts through the modulation of rhizosphere microbial community. [ABSTRACT FROM AUTHOR]

This study investigated whether hot water immersion (HWI) could heat acclimate athletes and improve intermittent running performance and perception of in-game running ability, during a competitive Australian Rules Football (ARF) season. Fifteen male semi-professional ARF athletes (Mean (SD); age: 22 (3) years, height: 182.3 (6.5) cm, mass: 80.5 (5.1) kg) completed either HWI (HEAT, N = 8, 13 (2) sessions, 322 (69) min exposure, 39.5 (0.3) °C) or acted as a control (CON, N = 7, no water immersion) over 6-weeks. Athletes completed a 30–15 Intermittent Fitness Test pre and post-intervention to assess intermittent running performance (VIFT), with perception of in-game running ability measured. Heat acclimation was determined via change in resting plasma volume, as well as physiological and perceptual responses during HWI. HEAT elicited large PV expansion (mean ± 90% CI: d = 1.03 ± 0.73), large decreases in heart rate (d = -0.89 ± 0.70), thermal sensation (d = -2.30 ± 1.15) and tympanic temperature (d = -1.18 ± 0.77). Large improvements in VIFT were seen in HEAT (d = 1.67 ± 0.93), with HEAT showing a greater improvement in VIFT when compared to CON (d = 0.81 ± 0.88). HEAT also showed greater belief that in-game running ability improved post-intervention (d = 2.15 ± 1.09) compared to CON. A 6-week HWI intervention can elicit heat acclimation, improve perception of in-game running ability, and potentially improve VIFT in semi-professional ARF athletes. [ABSTRACT FROM AUTHOR]

To describe the quality of life of women at an increased risk of ovarian cancer undergoing risk-reducing bilateral salpingo-oophorectomy (RRBSO). Patients evaluated in our gynecologic oncology ambulatory practice between January 2018–December 2019 for an increased risk of ovarian cancer were included. Patients received the EORTC QLQ-C30 and PROMIS emotional and instrumental support questionnaires along with a disease-specific measure (PROM). First and last and pre- and post-surgical PROM responses in each group were compared as were PROMs between at-risk patients and patients with other ovarian diseases. 195 patients with an increased risk of ovarian cancer were identified, 155 completed PROMs (79.5%). BRCA1 or BRCA2 mutations were noted in 52.8%. Also included were 469 patients with benign ovarian disease and 455 with ovarian neoplasms. Seventy-two at-risk patients (46.5%) had surgery and 36 had both pre- and post-operative PROMs. Post-operatively, these patients reported significantly less tension (p = 0.011) and health-related worry (p = 0.021) but also decreased levels of health (p = 0.018) and quality of life <7d (0.001), less interest in sex (p = 0.014) and feeling less physically attractive (p = 0.046). No differences in body image or physical/sexual health were noted in at-risk patients who did not have surgery. When compared to patients with ovarian neoplasms, at-risk patients reported lower levels of disease-related life interference and treatment burden, less worry, and better overall health. In patients with an increased risk of ovarian cancer, RRBSO is associated with decreased health-related worry and tension, increased sexual dysfunction and poorer short-term quality of life. Patients with ovarian neoplasms suffer to a greater extent than at-risk patients and report higher levels of treatment burden and disease-related anxiety. • Patients at risk of ovarian cancer experience less health-related worry and tension after risk-reducing surgery. • RRSO is associated with decreased short-term quality of life and higher levels of sexual dysfunction. • Patients with ovarian neoplasms suffer more more than at-risk patients and report higher levels of treatment burden and anxiety. [ABSTRACT FROM AUTHOR]

As part of his government responsibilities as Administrator and Lieutenant Governor in British New Guinea (1888-1898), Sir William MacGregor amassed a substantial group of ethnographic objects currently known as the 'Official' collection and housed mainly at the Papua New Guinea National Museum and Art Gallery and the Queensland Museum. Alongside this activity, MacGregor also set aside some material for his own private use, referred to here as the 'Personal' collection. The majority of the Personal collection was later donated to the University of Aberdeen to encourage fellow Scots to broaden their knowledge of the wider world. A smaller component that had been gifted to friends and colleagues is now housed at the Cambridge University Museum of Archaeology and Anthropology and several other museums. Many of the objects MacGregor reserved for himself probably had special significance as memories of people who became his friends and of occasions when he achieved success in his goal to bring what he considered as 'civilisation' to the peoples in the colony. Other highly decorated items that would have been attractive to nineteenth-century collectors were gifted by MacGregor to friends or high-status individuals to cement personal relationships or gain prestige. A comparison of the items reserved for MacGregor's private use with the British New Guinea Official collection highlights key differences between nineteenthcentury ethnographic collections intended as comprehensive 'scientific' data versus those, like MacGregor's Personal collection, whose contents were designed chiefly for the education and enjoyment of its owners. [ABSTRACT FROM AUTHOR]

This chapter is concerned with the meanings and purposes of the highly varied arrows and bows that were collected for the Official collection from the Western Division of British New Guinea during Sir William MacGregor's administration (1888-1898). As the data about provenance vital to their understanding has not been preserved, these objects largely remain in museum storage and unfortunately have not contributed to an understanding of the history and relationships surrounding their acquisition. Although commonly understood and displayed as weapons by colonial administrations, twentieth century research has emphasised their role in connecting disparate people through an extensive trade network and a shared mythic landscape. This focus on the uses and meanings, rather than the origin and fabrication, of arrows is discussed in the chapter to direct attention towards the events and relationships that instigated their inclusion in the Official collection. [ABSTRACT FROM AUTHOR]

This chapter describes the ways that collections were made in British New Guinea during Sir William MacGregor's tenure (1888-1898) through a focus on the people employed in field collecting for bird specimens. It makes explicit the involvement of local peoples and other specialist collectors living in the region at the time. The creation of the collections was thus through two knowledge systems: that of western science and those of British New Guinean peoples. The influence of locals and other people is evident in the shape of the collection and the kinds of birds acquired. I argue that the curation of the bird specimens at Queensland Museum divorced specimens from the human contexts which contributed to reinforcing the hierarchical colonial structure. Despite this, examination of the collection's composition shows the focus on 'beautiful and gorgeous' species which emphasises the knowledge of British New Guinea's people and reveals the larger and more complex sphere of social relations that were a feature of MacGregor's tenure. [ABSTRACT FROM AUTHOR]

Context: Perennial forage grass species are often grown with limited water following establishment and rely on accessing water deep in the soil profile to survive. Aim: This study aimed to characterise bermudagrass (Cynodon spp.) genotypes with rapid vertical root growth associated with post-establishment survival. Methods: Twelve bermudagrasses representing genotypes from diverse climate zones in Australia were established in rhizotrons to analyse the stability in genotypic variation in root and shoot growth in winter and summer experiments. Genotypic rank of root length, leaf area, and root dry weight were consistent in both seasons. Key results: Bermudagrass genotypes exhibited different traits correlated with root vertical growth rate and inconsistency of genotypic rank of shoot growth. During winter establishment, the rate of root depth development (RRDD) (r = −0.64) was correlated with the proportion of root length that became inactive, that was likely due to seasonal root death in winter conditions; during summer establishment, RRDD was correlated with tiller appearance rate (r = 0.45) and root distribution to 10 cm depth (r = −0.62). Shoot dry weight was correlated with photosynthesis (r = 0.85) and transpiration (r = 0.79) in summer, but not in winter. RRDD (r = 0.75, winter and r = 0.77, summer) was correlated with drought resistance index, previously analysed under field conditions. Conclusions and implications: Genotypes from the Mediterranean climates in Australia showed rapid growth of roots and shoots in both seasons and have the greatest potential for broader application for forage production in variable environments. Perennial forage plants persist in the field indefinitely, so the growth during establishment is important to determine the whole lifecycle. Here, we studied how fast the root elongated downward among different grasses of the same species, and found root activity and shoot growth could affect the root elongation rate. Our finding implied that the selection for grasses with specific traits can improve the forage establishment over a shorter time. [ABSTRACT FROM AUTHOR]

The objective of this study was to determine the proportion of patients with a pre-invasive endometrial lesion who meet Mayo criteria for lymph node dissection on final pathology to determine if the use of sentinel lymph node biopsy in patients with pre-invasive lesions would be warranted. All women who underwent hysterectomy for a pre-invasive endometrial lesion (atypical hyperplasia or endometrial intra-epithelial neoplasia) between 2009 and 2019 were included for analysis. Relevant statistical tests were utilized to test the associations between patient, operative, and pathologic characteristics. 141 patients met inclusion criteria. 51 patients (36%) had a final diagnosis of cancer, the majority (96%) of which were Stage IA grade 1 endometrioid carcinomas. Seven patients (5%) met Mayo criteria on final pathology (one grade 3, seven size >2 cm, one >50% myoinvasive). Three of these seven patients had lymph nodes assessed of which 0% had metastases. Six of these patients had frozen section performed, and 2 met (33%) Mayo criteria intraoperatively. Of the seven patients in the overall cohort that had lymph node sampling, six had a final diagnosis of cancer and none had positive lymph nodes. Of the 51 patients with cancer, only 10 had cancer diagnosed using frozen section, and only two met intra-operative Mayo criteria. Age > 55 was predictive of meeting Mayo criteria on final pathology (p = 0.007). No patients experienced a cancer recurrence across a median follow up of 24.3 months. Atypical hyperplasia and endometrial intra-epithelial neoplasia portend low risk disease and universal nodal assessment is of limited value. • Among our cohort of patients with AH/EIN, 5% met Mayo criteria on final pathology. • No patients were found to have positive lymph nodes or subsequent nodal recurrence. • AH/EIN portend low risk disease and universal nodal assessment is of limited value. [ABSTRACT FROM AUTHOR]

Laboratory-generated bioaerosols are widely used in aerobiology studies of viruses; however, few comparisons of alternative nebulizers exist. We compared aerosol production and virus survival for a Collison nebulizer, vibrating mesh nebulizer (VMN), and hydraulic spray atomizer (HSA). We also measured the dry size distribution of the aerosols produced and calculated the droplet sizes before evaporation and the dry size distribution from normal saline solution. Dry count median diameters of 0.11, 0.22, and 0.30mm were found for normal saline from the Collison nebulizer, VMN, and HSA, respectively. The volume median diameters were 0.323, 1.70, and 1.30mm, respectively. The effect of nebulization on the viability of two influenza A viruses (IAVs) (H1N1 and H3N2) and human rhinovirus 16 (HRV-16) was assessed by nebulization into an SKC BioSampler. The HSA had the least impact on surviving fractions (SFs) of H1N1 and H3N2 (89% ± 63% and 94% ± 62%, respectively), followed by the Collison nebulizer (83% ± 61% and 82% ± 62%, respectively). The VMN yielded SFs of 78% ± 62% and 76% ± 62%, respectively. Conversely, for HRV-16, the VMN produced higher SFs (87% ± 68%). Our findings indicate that there were no statistical differences between SFs of the viruses nebulized by these nebulizers. However, VMN produced higher aerosol concentrations within the airborne size range, making it more suitable where high aerosol mass production is required. [ABSTRACT FROM AUTHOR]

Hyperleptinaemia is a well-established therapeutic side effect of drugs inhibiting the androgen axis in prostate cancer (PCa), including main stay androgen deprivation therapy (ADT) and androgen targeted therapies (ATT). Given significant crossover between the adipokine hormone signalling of leptin and multiple cancer-promoting hallmark pathways, including growth, proliferation, migration, angiogenesis, metabolism and inflammation, targeting the leptin axis is therapeutically appealing, especially in advanced PCa where current therapies fail to be curative. In this study, we uncover leptin as a novel universal target in PCa and are the first to highlight increased intratumoural leptin and leptin receptor (LEPR) expression in PCa cells and patients' tumours exposed to androgen deprivation, as is observed in patients' tumours of metastatic and castrate resistant (CRPC) PCa. We also reveal the world-first preclinical evidence that demonstrates marked efficacy of targeted leptin-signalling blockade, using Allo-aca, a potent, specific, and safe LEPR peptide antagonist. Allo-aca-suppressed tumour growth and delayed progression to CRPC in mice bearing LNCaP xenografts, with reduced tumour v ascularity and altered pathways of apoptosis, transcription/translation, and energetics in tumours determined as potential mechanisms underpinning anti-tumour efficacy. We highlight LEPR blockade in combination with androgen axis inhibition represents a promising new therapeutic strategy vital in advanced PCa treatment. [ABSTRACT FROM AUTHOR]

Aims: Perennial forage grass production has the potential to improve smallholder livelihoods in the tropics. However, nutrient management is often challenging, especially on infertile sandy soils. This study tested whether typical nutrient management limits the productivity and sustainability of perennial forage grass systems on sandy soils. Methods: Nutrient balances were estimated for four fields of either Megathyrsus maximus cv. Tanzania or Urochloa hybrid Mulato II. Nutrient limitations were then evaluated in a nutrient omission experiment. Results: All soils were sandy (< 10% clay), neutral to acidic (pH (CaCl2) 4.6 to 6.7) and had plant-limiting concentrations of total nitrogen, extractable potassium and extractable sulfur. Nitrogen inputs were typically higher than outputs, with balances ranging from −16 kg ha−1 yr−1 to 293 kg ha−1 yr−1, yet concentrations in forage shoots were low at all sites. Phosphorus balances ranged from −5 kg ha−1 yr−1 to 77 kg ha−1 yr−1 and concentrations in forage shoots were adequate. Potassium inputs were low, resulting in balances from −79 kg ha−1 yr−1 to −138 kg ha−1 yr−1 at locations that did not apply inorganic potassium fertilizer. Potassium concentrations in forage shoots were low and omission of potassium resulted in severely depressed biomass production. Inorganic sulfur fertilizers were not applied to forages and the sulfur balance varied from −1 kg ha−1 yr−1 to −24 kg ha−1 yr−1. Sulfur concentrations in shoots were correspondingly low and production was depressed when sulfur was omitted in the experiment. Conclusion: Balanced nutrition was not achieved, despite substantial fertilizer inputs, resulting in deficiencies of potassium and sulfur, inefficient use of nitrogen and excesses of phosphorus. If current practices continue, potassium and sulfur depletion, phosphorus accumulation and soil acidification can be expected. Recommendations for balanced nutrient management that accounts for high rates of removal in biomass, variable concentrations in organic fertilizers, and leaching potential, are needed to sustain the productivity of perennial forages on tropical sandy soils. [ABSTRACT FROM AUTHOR]

Background: Currently the diagnosis of shoulder instability, particularly in children, is difficult and can take time. These diagnostic delays can lead to poorer outcome and long-term complications. A Diagnostic Decision Support System (DDSS) has the potential to reduce time to diagnosis and improve outcomes for patients. The aim of this study was to develop a concept map for a future DDSS in shoulder instability.Methods: A modified nominal focus group technique, involving three clinical vignettes, was used to elicit physiotherapists decision-making processes.Results: Twenty-five physiotherapists, (18F:7 M) from four separate clinical sites participated. The themes identified related to 'Variability in diagnostic processes and lack of standardised practice' and 'Knowledge and attitudes towards novel technologies for facilitating assessment and clinical decision making'.Conclusion: No common structured approach towards assessment and diagnosis was identified. Lack of knowledge, perceived usefulness, access and cost were identified as barriers to adoption of new technology. Based on the information elicited a conceptual design of a future DDSS has been proposed. Work to develop a systematic approach to assessment, classification and diagnosis is now proposed. Trial Registraty This was not a clinical trial and so no clinical trial registry is needed. [ABSTRACT FROM AUTHOR]

The objective was to determine if surgical approach affects time to recurrence in early-stage high-intermediate risk endometrial cancer (HIR-EC) treated with adjuvant vaginal brachytherapy (VBT). In this retrospective cohort study, HIR-EC patients treated with VBT between 2005 and 2017 were identified and those who received open or minimally invasive hysterectomies (MIS) were included. Clinical and surgical variables were analyzed and time to recurrence was compared between surgical groups. We identified 494 patients, of which 363 had MIS hysterectomies, 92.5% had endometrioid histology, 45.7% were stage IA and 48.0% stage IB. Open hysterectomy patients had higher BMIs (p = 0.007), lower rates of lymph node sampling (p < 0.001) and lymphovascular space invasion (LVSI) (p = 0.036), however in patients who recurred, no differences were noted between groups. Overall, 65 patients (13.2%) recurred, 14 in the open group (10.7%) and 51 in the MIS group (14.0%) (p = 0.58), while vaginal recurrences were noted in 4.6% and 6.1% respectively. When compared to the open group, the MIS group had a significantly shorter time to any recurrence (p = 0.022), to pelvic (p = 0.05) and locoregional recurrence (p = 0.021) and to death from any cause (p = 0.039). After adjusting for age, BMI, grade, LVSI and surgery date, the MIS group had a higher risk of any recurrence (HR 2.29 (1.07–4.92), p = 0.034) and locoregional recurrence (HR 4.18 (1.44–12.1), p = 0.008). Patients with HIR-EC treated with VBT after MIS hysterectomy have a shorter time to recurrence and higher risk of recurrence when compared to open hysterectomy patients. Further studies into the safety of MIS in high-intermediate risk patients are required. • In patients with high-intermediate risk endometrial cancer, mode of surgery impacts risk of recurrence after brachytherapy • Minimally invasive surgery results in a shorter time to overall and locoregional recurrence when compared to open surgery • Minimally invasive surgery results in a higher risk of overall and locoregional recurrence when compared to open surgery [ABSTRACT FROM AUTHOR]

A conventional empirical orthogonal function (EOF) analysis is performed on summertime (May-October) western North Pacific (WNP) tropical cyclone (TC) track density anomalies during 1970-2012. The first leading EOF mode is characterized by a consistent spatial distribution across the WNP basin, which is closely related to an El Niño-Southern Oscillation (ENSO)-like pattern that prevails on both interannual and interdecadal time scales. The second EOF mode is represented by a tripole pattern with consistent changes in westward and recurving tracks but with an opposite change for west-northwestward TC tracks. This second EOF pattern is dominated by consistent global sea surface temperature anomaly (SSTA) patterns on interannual and interdecadal time scales, along with a long-term increasing global temperature trend. ObservedWNP TC tracks have three distinct interdecadal epochs (1970-86, 1987-97, and 1998-2012) based on EOF analyses. The interdecadal change is largely determined by the changing impact of ENSO-like and consistent global SSTA patterns. When global SSTAs are cool (warm) during 1970-86 (1998-2012), these SSTAs exert a dominant impact and generate a tripole track pattern that is similar to the positive (negative) second EOF mode. In contrast, a predominately El Niño-like SSTA pattern during 1987-97 contributed to increasing TC occurrences across most of theWNPduring this 11-yr period. These findings are consistent with long-term trends in TC tracks, with a tripole track pattern observed as global SSTs increase. This study reveals the potential large-scale physical mechanisms driving the changes of WNP TC tracks in association with climate change. [ABSTRACT FROM AUTHOR]

Jurisdictions around the globe are working to address climate change and many municipalities are seeking to protect their communities from its impacts. Although nearly half the world's population resides in rural areas, most municipal climate change planning literature focuses on urban municipalities. To that end, this paper analyzes the public policy process of Nova Scotia, Canada's rural Municipal Climate Change Action Planning mandate. Through an analysis of the plans and follow-up interviews with municipal planners, we examine the conditions that sustain local climate planning and what municipalities gleaned from this climate planning process. [ABSTRACT FROM AUTHOR]

I examine the ways in which reading in the classroom is a social practice and how that social practice can produce nuanced student texts that reflect a rich understanding of what has been read. In doing so, I also consider how the reading environment allows us to manipulate and embody texts in ways that allow for a more detailed understanding than the individual and solitary reading practices that seem to be prized in our society. [ABSTRACT FROM AUTHOR]

Semiselective heteronuclear cross-polarization for achieving coherence transfer between a spin I = ½ scalar coupled to a spin S ≥ ½ in isotropic solution is considered. The expansion of the density operator as products of irreducible tensor operators provides a compact formalism for describing cross-polarization involving scalar coupled quadrupolar nuclei. An analytical description of cross-polarization is presented in the limit of strong radio-frequency (rf) fields, with respect to the scalar-coupling constant. Numerical simulations show that reducing the rf field amplitudes does not have a detrimental effect on the efficiency of the transfer provided they are comparable to or greater than the scalar-coupling constant. The use of weak rf fields largely circumvents the reduced efficacy due to Hartmann-Hahn mismatch. Applications of the method for selective observation of scalar-coupled quadrupolar nuclei are considered and experimental results are presented for a mixture of beryllium fluoride complexes. [ABSTRACT FROM AUTHOR]

In nuclear magnetic resonance (NMR) of I=1/2 nuclei that are scalar coupled to quadrupolar spins, a tensor operator product (TOP) basis set provides a convenient description of the time evolution of the density operator. Expressions for the evolution of equivalent I=1/2 spins, coupled to an arbitrary spin S>1/2, were obtained by explicit algebraic density operator calculations in Mathematica, and specific examples are given for S=1 and S=3/2. Tensor operators are described by the convenient quantum numbers rank and order and this imparts to the TOP basis features that enable an intuitive understanding of NMR behavior of these spin systems. It is shown that evolution as a result of J coupling alone changes the rank of tensors for the coupling partner, generating higher-rank tensors, which allow efficient excitation of S-spin multiple-quantum coherences. Theoretical predictions obtained using the TOP formalism were confirmed using multiple-quantum filtered heteronuclear spin-echo experiments and were further employed to demonstrate polarization transfer directly to multiple-quantum transitions using the insensitive nucleus enhancement by polarization transfer pulse sequence. This latter experiment is the basis of two-dimensional heteronuclear correlation experiments and direct generation of multiple-quantum S-spin coherences can therefore be exploited to yield greater spectral resolution in such experiments. Simulated spectra and experimental results are presented. © 2001 American Institute of Physics. [ABSTRACT FROM AUTHOR]

Shoulder instability (subluxations and dislocations) in young people causes pain and disability. Recurrence rates are between 30%-90%, and long-term complications such as premature glenohumeral joint arthropathy are common [1]. Identification of movement and muscles activity differences may help inform clinical-decision making and improve patient outcomes. Is it possible to identify differences in movement and muscle activity patterns between children with and without shoulder instability using 3D-movement analysis and surface electromyography (sEMG)? Participants attended a single measurement session. Participants' movements were assessed during four unweighted (flexion, abduction, axial-rotation and hand to head) and self-selected weighted tasks of 0.5 kg, 1.0 kg or 1.5 kg (flexion, abduction, axial-rotation). Movement data was collected at 100 Hz using 12 V5-Vantage motion analysis cameras and two synchronous coronal and sagittal video recordings. Delsys Trigno electromyography system sampling at 2000hz was used to record sEMG. Retroreflective marker clusters were placed on the thorax, acromion and upper-limb segments [2]. Joint angles were calculated using inverse kinematics and the Wu shoulder model in Opensim 4.3 [3–5]. sEMG electrodes were placed on middle-trapezius, infraspinatus, triceps, latissimus-dorsi, deltoid (posterior and anterior), pectoralis-major, biceps, wrist-flexor and extensor muscles. Statistical parametric mapping was used to identify between-group differences in 3D-joint angles and muscle activations, for all tasks. Data was collected for 30 children (15 with shoulder instability (6 M:9 F) and 15 sex- and age-matched controls (8 M:7 F)). The shoulder instability cohort had mean (SD) age, height and weight values of 13.9 years (2.8), 163.0 cm (15.2) and 56.6 kg (16.9) respectively. The reference cohort had age, height and weight values of 13.3 years (3.0), 160.6 cm (16.2) and 52.4 kg (14.6) respectively. Fig. 1 shows 3D shoulder joint angles across all tasks. Statistically significant differences were observed in 51/56 (91%) of joint angle-task combinations. [Display omitted] Consistent differences between groups were seen across all activities for glenohumeral elevation angle, sternoclavicular protraction/retraction and elevation/depression angles and acromioclavicular internal/external rotation and tilt. All upper-limb joint angles were significantly different between groups for the tasks of abduction, abduction with weight, axial-rotation and hand to head. For all muscle and task combinations, statistically significant differences were observed in 36/56 (64%). Posterior deltoid and middle-trapezius were most frequently identified as being different across tasks (n=6/7). Pectoralis major was the only muscle not identified as being statistically significantly different across all tasks. The 3D-movement analysis and sEMG protocol is able to identify muscle activity and movement pattern differences between young people with and without shoulder instability. Whilst overall arm position appeared similar, preliminary results suggest that significantly different movement strategies across the shoulder-girdle joints and associated muscles may be used by those with shoulder instability. Further work is needed to identify if these differences can differentiate between those at risk of future instability. [ABSTRACT FROM AUTHOR]

Facioscapulohumeral dystrophy (FSHD) can affect upper-limb function through muscle degeneration, fatty infiltration and oedema. Muscle echogenicity, measured using ultrasound, could be used as a biomarker for muscular changes and disease progression [1–3]. Histogram-matching may be instrumental in overcoming existing shortcomings that prevent us from using such images to extract clinically useful information [4,5]. Can histogram-matching of muscle ultrasound images be used to extract clinically relevant measures to quantify the muscle morphological changes in people with FSHD (pwFSHD)? Participants attended a single motion analysis session for upper-limb 2D-ultrasound imaging and 3D-movement analysis. Stratified sampling by arm function was used for pwFSHD. Controls were age and sex matched. Middle trapezius measurement was taken at the midpoint of a line between C7 and ACJ. Six total measurements were taken (3-longitudinal and 3-transverse views) using an Esoate MyLab-Gamma device and linear probe (3-13 MHz). Muscle thickness measurements were carried using ImageJ 1.53t. Histogram-matching was carried out as described by Bottenus et al. [4]. All images were matched to a single reference image from a control group participant. Manual segmentation of the subcutaneous fat layer was carried out and used as the region-of-interest for histogram-matching across all images. Using full histogram-matching, the monotonic transformation was applied across the entire image. The trapezius muscle was segmented to determine mean grayscale values (echogenicity). The student t-test was used for evaluating between group differences and the relationship between echogenicity values and muscle thickness was investigated. Data was collected for 14 participants (7 pwFSHD (2 F:5 M) and 7 sex- and age-matched controls (2 F:5 M). PwFSHD had mean (SD) age, height and weight values of 41.9-years (17.1), 176 cm (8.8) and 90.6 kg (24.8) respectively. The control group had age, height and weight values of 41.4-years (15.5), 176.4 cm (5.7) and 77.1 kg (11.2) respectively. Group echogenicity values are presented in Fig. 1. [Display omitted] Mean (SD) echogenicity values for pwFSHD were higher than the control group (96.5 (30.3) vs 32.2 (11.2) respectively) with statistically significant differences (p<0.001). Mean (SD) trapezius muscle thickness was higher in the control group 1.48 cm (0.27) vs 0.74 cm (0.45) respectively. Mean echogenicity scores accounted for 82% of the variance in mean muscle thickness values (R2=0.824). PwFSHD demonstrated higher echogenicity values and smaller muscle thicknesses indicative of degenerative muscle structure changes associated with the disease. Preliminary results suggest that post capture processing of ultrasound images using histogram matching can provide quantifiable differences in people with and without FSHD. This could facilitate clinically feasible bedside methods for assessing and monitoring disease progression in pwFSHD. Further work is needed to recruit a larger sample of pwFSHD and varying levels of arm function, carry out longitudinal measurements and evaluate the sensitivity of these measures on the basis of variable reference images. [ABSTRACT FROM AUTHOR]

Hereditary hemorrhagic telangiectasia (HHT) is a disorder characterized by vascular manifestations including mucocutaneous and visceral telangiectasias and arteriovenous malformations. Herein we present the case of a relatively young patient with HHT with an incidentally discovered locally advanced esophageal cancer on endoscopic screening and pathologically complete response after neoadjuvant chemoradiation. This case highlights an unusual tumor response to chemoradiation in locally advanced esophageal cancer, and the surveillance care of HHT patients. [ABSTRACT FROM AUTHOR]

In this essay, I draw on the experience of reading Hamlet with a class of 11- and 12-Year-old boys in a London comprehensive school. I explore how a number of comments in my classroom reveal the complexities of my students' thinking about gender and sexuality in Hamlet. I look at how these thoughts are difficult for the students to fully express, and I argue that it is necessary to attend to the heteroglossic nature of the language used within the classroom. [ABSTRACT FROM AUTHOR]

Bournemouth Borough Council in the UK implemented two shared-space schemes under the Local Sustainable Transport Fund programme at Horseshoe Common and at Boscombe, completed in 2014 and 2015, respectively. This paper outlines the process of developing the schemes, including the consultation, design and construction of the schemes. It also describes some of the user feedback and lessons learnt and it discusses some of the merits of shared-space schemes, making recommendations to practitioners while also raising questions for further consideration. [ABSTRACT FROM AUTHOR]