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[Display omitted] Ion channel activation upon ligand gating triggers a myriad of biological events and, therefore, evolution of ligand gating mechanism is of fundamental importance. TRPM2, a typical ancient ion channel, is activated by adenosine diphosphate ribose (ADPR) and calcium and its activation has evolved from a simple mode in invertebrates to a more complex one in vertebrates, but the evolutionary process is still unknown. Molecular evolutionary analysis of TRPM2s from more than 280 different animal species has revealed that, the C-terminal NUDT9-H domain has evolved from an enzyme to a ligand binding site for activation, while the N-terminal MHR domain maintains a conserved ligand binding site. Calcium gating pattern has also evolved, from one Ca2+-binding site as in sea anemones to three sites as in human. Importantly, we identified a new group represented by olTRPM2, which has a novel gating mode and fills the missing link of the channel gating evolution. We conclude that the TRPM2 ligand binding or activation mode evolved through at least three identifiable stages in the past billion years from simple to complicated and coordinated. Such findings benefit the evolutionary investigations of other channels and proteins. [ABSTRACT FROM AUTHOR]

Vocalizations that signal predation risk such as alarm calls provide crucial information for the survival of group-living individuals. However, alarm calling may attract the predator's attention and, to avoid this cost, animals can opt for alternative strategies to indicate danger, such as 'adaptive silence', which is the cessation of vocalizations. We investigate here whether abrupt contact call cessation would provoke alarm responses, or would reinforce the signal given by an alarm call. In an aviary setting, we conducted playback experiments with a group-living passerine, the Swinhoe's white-eye, Zosterops simplex. We found that birds did not respond to a sudden call cessation, nor did they have a stronger response to alarm calls followed by silence than to alarm calls followed by contact calls. Confirming previous work investigating contact call rate, it appears that in this species contact calls encode information about social factors but not environmental conditions. [ABSTRACT FROM AUTHOR]

Background: Children Snoring is a common childhood disorder that affects the growth and development of children and is detrimental to their health. Increasing awareness of Children Snoring among parents is important. Aim: To develop the Knowledge-Attitude-Practice of Parents towards Children Snoring Scale and test the reliability and validity of the scale. Methods: The development of the tool was divided into two phases involving 1257 parents from China. In the first phase, an initial project bank was created through a literature review. This was followed by a Delphi expert consultation, group discussion and pre-survey. The second stage screened the items and conducted an exploratory factor analysis, then conducted a confirmatory factor analysis and tested for reliability and validity. Results: Support was found for the 25-item Knowledge-Attitude-Practice toward Children Snoring scale. Exploratory and confirmatory factor analyses provide support for four subscales: (parental basic cognition toward Children Snoring; parents' perception of complications of Children Snoring; parents' attitude towards Children Snoring; parents' concern and prevention of Children Snoring). Internal consistency for the total scale was high (Cronbach's α = 0.93). The intraclass correlation coefficient of test-retest reliability was 0.92 (95%CI: 0.85 to 0.95), which provided support for the stability of the scale. Conclusion: The Knowledge-Attitude-Practice of Parents towards Children Snoring scale shows promise as a measure that may be used by medical workers and community children's health managers. [ABSTRACT FROM AUTHOR]

An application method for working ship crossing safely is proposed to determine how to make navigation scheme at a certain time. This method makes it possible for decision makers to make reasonable judgments at different times. In this paper, the position relationship between working ship and navigation vessel in waterway is analysed by considering the ship size, hydrological conditions of waterway, ship arrival model and ship navigation trajectory. Using genetic algorithm, the operation scheme of keeping a safe distance between the working ship and the vessel in the channel is solved by taking the speed and direction of the working ship as genetic factors. By analysing the crossing scheme at each starting time in a given time range, the optimal crossing scheme with the farthest distance between the working ship and the vessels in the channel is obtained. According to the measured data, the simulation is carried out with MATLAB to verify the model of working ship crossing channel. The results show that it is safe and reliable to choose the navigation scheme proposed in this paper, which has strong application value. [ABSTRACT FROM AUTHOR]