1. Simple sequence repeat markers and physiological characterization of some local Tunisian barley accessions.
- Author
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Raoudha, Abdellaoui, Hatem, Cheikh M'hamed, M'barek, Ben Naceur, Chaabane, Rahmoune, Bettaïb-Kaab Leîla, and Jeannette, Ben Hmida
- Subjects
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BIOLOGICAL variation , *GENETICS , *BARLEY , *PLANTS , *BREEDING - Abstract
The genetic variation and relationships among 13 local barleys and the variety Martin were evaluated using Simple Sequence Repeat (SSR) markers and physiological traits. The molecular study showed that a total of 43 bands were generated by 15 SSR primers, with an average of 2.6 easily detectable bands per primer. Of these 43 fragments, only 39 (90.7%) were polymorphic among the 14 barleys studied. Within-accessions genetic variation dissimilarity ranged from 15% to 77.78% of the total. The important dissimilarities were observed between East-Northern accessions and Southern ones, whereas the variety Martin showed low dissimilarity with the East Northern ones. Cluster analysis indicated that most of the accessions could be clustered into groups, which basically coincided with their geographical distribution. Also, physiological results indicated geographic information. Although comparison of physiological and molecular data using the Mantel test indicated a low correlation (r = 0.276), both methods showed a high degree of variation among analyzed accessions, indicating an important source of genetic diversity that can be used in future breeding programs. [ABSTRACT FROM AUTHOR]
- Published
- 2007