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Objectives: the goal of the study was to identify the relationship between horizontal and vertical jumping tests with the incidence of lower limb musculoskeletal injuries in soccer players of a professional league club in Colombia. Materials and methods: an analytical, prospective study was carried out on 30 players from the professional roster of Club Deportivo Atlético Junior F.C. At the beginning of the season the anthropometric characteristics and body composition were evaluated, as well as jumping and functional asymmetries of the lower extremities through vertical (CMJ and CMJs) and horizontal (3-Hop-Test) jumping tests. The analysis consisted in comparing the records of the variables studied between the players with (injured n=11) and without injury (not-injured n=19) during the first semester of the 2019 season. Results: no statistical differences were found between the groups in the biological, anthropometric and body composition characteristics (p> 0.05). Derived from the vertical jumping findings, no significant differences were observed in the asymmetries between groups (p> 0.05), however, significant asymmetries were found in the horizontal jumping tests between injured and uninjured players (p <0.01). Conclusion: from this study we can conclude that unlike uninjured professional soccer players, there were significantly greater functional asymmetries identified through the horizontal jumping test in athletes with injuries. [ABSTRACT FROM AUTHOR]