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Endotracheal intubation in rats is challenging due to the difficult anatomical characteristics of the airway. The success rate at first attempt is low and airway damage is a common complication. We aimed to compare and evaluate the conventional intubation method with a modified procedure using an inclined plate, headlamp (700-Lumen), and 3D mouth-piece designed with a 20° curvature. Both techniques were conducted by laboratory personnel with and without previous experience in airway management of laboratory rats. In this study, we used 36 Wistar rats of both genders. Three groups of laboratory personnel (anesthesiologists, medical students, and laboratory technicians) performed both endotracheal intubation techniques, i.e., blind intubation at supine position and endotracheal intubation at 70° supine position with a 3D mouth-piece and direct illumination of the glottis. The modified technique had a significantly higher success rate and shorter procedure duration. Moreover, there was no significant difference in the procedure duration between personnel with and without previous training in airway management. Previous knowledge and experience in airway management are required when performing conventional endotracheal intubation; moreover, its success rate is low. Contrastingly, using proper instruments and the 3D mouth-piece facilitated easier and quicker airway management regardless of previous experience. [ABSTRACT FROM AUTHOR]

Primary education in Mexico is facing a growing set of challenges that the government has tried to counteract through the use of communication technologies (ICT) in formal education. While these efforts provide support for students and educators, there remains a need for a renewed and contextualized awareness that will re-conceptualize the adverse experiences of students and the importance of resilience in the context of the learning environment. The objective of this document is to give an account of the learning acquired by nine telesecondary students in a rural area of Hidalgo and the student's benefits of building awareness about the ecology of learning. The paper highlights the process of re-envisioning their experiences that emerged from the various points of views shared in discussion. The study was triangulated by quantitative and qualitative phenomenological and hermeneutical analyses. It was organized into three stages and employed a survey, semi-structured interviews, focus groups and use of the Google-Classroom platform. The hermeneutical analysis of autobiographies and the use of technological resources enhanced the personal analysis of the experiences of the participants. These experiences generated learning that may often be invisible in formal education but can empower critical thinking, collaboration and autonomy of students to become aware of their learning and the scope of their social contribution. [ABSTRACT FROM AUTHOR]

Glucose-6-phosphate dehydrogenase deficiency (D-G6PD) is a common erythroenzymopathy that needs to be addressed as an important public health issue. Proper population monitoring is needed to anticipate clinical complications. A joint venture between Genomi-k (a Mexican company focused on newborn screening) and several university researchers conducted a retrospetive study for D-G6PD based on 156,152 newborn screening reports belonging to the Mexican population comprising a period of 10 years. We identified 540 male newborns affected with this deficiency, representing an incidence of 6.78 cases per 1,000 newborn males. A single double mutation of G202A:A376G was detected in 97.22% of cases. In regions where there is an absence of a national centralized health data for D-G6PD, information from a non-probabilistic large population sample can be used as a national incidence subrogate. [ABSTRACT FROM AUTHOR]

Background: Elaborate an updated guideline of recommendations for the safe return to elective orthopedic surgery post COVID-19 pandemic. Methods: Bibliographic review of relevant global literature. Results: Recommendation guidelines with appropriate definitions of orthopedic elective surgery, correct contagion risk stratification for COVID-19, considerations for specific risk groups, hospital adaptations and anesthetic, intraoperative and postoperative special care for a safe restart of orthopedic elective surgery post COVID-19 pandemic. Conclusions: The safe restart of orthopedic elective surgery is possible as long as we take into consideration the appropriate recommendations, which we have summarized in this review. [ABSTRACT FROM AUTHOR]

The survival benefit associated with adjuvant external beam radiation therapy (EBRT), following mastectomy and breast conserving surgery, in the treatment of female patients with lymph node-positive (LN+) breast cancer is well established. Treatment of LN+ male breast cancer is largely extrapolated from randomized clinical trials and meta-analyses including females only. This study retrospectively examines the impact of adjuvant EBRT, after either mastectomy or breast conserving surgery, in LN+ male breast cancer patients using data from the National Cancer Database (NCDB). We hypothesize that a survival benefit will also be seen in LN+ male breast cancer patients receiving adjuvant EBRT. The NCDB was queried for male patients with a diagnosis of invasive breast cancer who were pathologically node positive from 2004 to 2017. Patients were excluded if they had metastatic disease at time of diagnosis or pathologically node negative disease. We included males irrespective of type of surgery performed, including mastectomy, lumpectomy, sentinel lymph node biopsy, and/or axillary lymph node dissection. Cohorts were separated based upon treatment with or without EBRT. Cox regression was used for multivariate survival analyses taking into account patient's insurance, Charlson-Deyo-Comorbidity score, hormone receptor status, age at diagnosis, number of LNs involved, and if adjuvant chemotherapy and/or hormone therapy were delivered. A total of 7,558 men were identified, with 3,852 (51%) having received EBRT (cohort 1a) and 3,706 who did not receive EBRT (cohort 1b). See the table below for some of the patient characteristics. Numbers listed in the table represent the total number of patients with percentages of each cohort in parenthesis. Five-year overall survival was 72.3% for those who received EBRT in comparison to 64.6% for those who did not receive EBRT. When using multi-variate survival analysis, the hazard ratio for cohort 1b as compared to cohort 1a was 1.30 (p <0.01), signifying that those who did not receive EBRT were 30% more likely to die in comparison to those who did receive EBRT. There appears to be an overall survival benefit associated with adjuvant EBRT in LN+ male breast cancer patients, despite those patients presenting with higher stage. Consideration for adjuvant radiation therapy in LN+ male breast cancer patients should be discussed. [ABSTRACT FROM AUTHOR]

Highlights: [•] Low-level acute cadmium exposure elicited tissue-specific gene expression changes. [•] Molecular initiating events included oxidative stress and disruption of DNA repair. [•] Metallothionein, a marker of metal exposure, was not significantly affected. [•] We report effects of cadmium on cholesterol metabolism and steroid synthesis. [•] Diabetic complications and impaired reproduction are potential adverse outcomes. [Copyright &y& Elsevier]

Bottlenose dolphins (Tursiops truncatus) are found in coastal and estuarine ecosystems where they are in continuous contact with multiple abiotic and biotic stressors in the environment. Due to their role as predators, they can bioaccumulate contaminants and are considered sentinel organisms for monitoring the health of coastal marine ecosystems. The northern zonal coast of the Yucatan peninsula of Mexico has a high incidence of anthropogenic activities. The principal objectives of this study were two-fold: 1) to determine the presence of trace metals and their correlation with lipids in bottlenose dolphin blubber, and 2) to use a lipidomics approach to characterize their biological responses. Levels of trace elements (Al, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Se, Cd, Pb) were analyzed using ICP-MS and lipids were measured using a targeted lipidomics approach with LC-MS/MS. Spearman correlation analysis was used to identify associations between lipids and trace elements. The influences of gender, stranding codes, presence of stomach content, growth stages and body length were also analyzed. Blubber lipid composition was dominated by triacylglycerols (TAG). Our results demonstrated the presence of heavy-metal elements such as Cd and As, which were correlated with different lipid species, mainly the ceramides and glycerophospholipids, respectively. Organisms with Cd showed lower concentrations of ceramides (CER, HCER and DCER), TAG and cholesteryl esters (CE). Trace elements Cr, Co, As and Cd increased proportionately with body length. This study provides a novel insight of lipidomic characterization and correlations with trace elements in the bottlenose dolphin which might contribute to having a better understanding of the physiological functions and the risks that anthropogenic activities can bring to sentinel organisms from coastal regions. [Display omitted] • Heavy metals in dolphin blubber were correlated with different lipid species. • The presence of Cd was linked to a decrease of ceramides. • Lipidomics analysis demonstrated that blubber was dominated by triacylglycerols. • This study provides a novel insight for lipidomics-trace metal correlations. [ABSTRACT FROM AUTHOR]

• Venom of P. volitans inhibits the growth of D. rerio embryos. • Venom of P. volitans affects dopaminergic neurons. • Venom of P. volitans mainly affects the α2 subunit of the nicotinic acetylcholine receptor. • Venom of P. volitans had an inhibitory effect on human neuronal α3β2 receptors. • Venom of P. volitans increased levels of acetylcholinesterase mRNA. Pterois volitans venom induces muscular fibrillation, which results from nerve transmission caused by the presence of acetylcholine (ACh). It also has cardiovascular effects that are due to its actions on muscarinic and nicotinic cholinergic receptors. In this study, we characterized the effects of P. volitans venom on nicotinic acetylcholine receptors (nAChRs) and dopaminergic neurons. After exposure to P. volitans venom, acetylcholinesterase (AChE) mRNA levels and the expression of the α2 subunit of nAChR increased in zebrafish embryos (15–20 somites). In addition, the lionfish venom blocked zebrafish α2 nAChR subunit functional expression and the ACh-induced response of human neuronal α3β2 receptors. The latter receptor was blocked by a protein fraction named F2, which was isolated from P. volitans venom using reversed phase high performance liquid chromatography (RP-HPLC). This venom causes death in dopaminergic neurons, and affects the cholinergic system. The effect of these two systems may result in retarded embryonic development of zebrafish, since the two systems function in a related manner to control growth hormone secretion. [ABSTRACT FROM AUTHOR]