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Indonesia has released some elite varieties of sugarcane that are suitable for Indonesian climatic and land conditions. Therefore, large quantities of these elite sugarcane seedlings are needed to support the Indonesia's sugar self-sufficiency programs. The large quantities seedlings can be provided through scaling-up in vitro propagation method using bioreactors, such as Temporary Immersion Bioreactor (TIB). Here, we conducted a study to analyze the response of different plant genetic materials on the in vitro propagation of some Indonesian elite sugarcane varieties using TIB. The sugarcane varieties used for the study were the rainfed sugarcane varieties (PSKA 942, PS 094) and the irrigated sugarcane varieties (PS 091, PS 881), released by the Indonesian Sugar Research Institute. The plant materials used as explants for in vitro propagation were the young spindle leaves isolated from the plant canes. The in vitro propagation stages were explants preparation and sterilization, callus initiation, callus proliferation and regeneration in TIB, shoot maturation, and acclimatization. Results showed that all sugarcane varieties have been successfully induced to form callus ranged 11.6 – 33.5%, proliferate and regenerate in TIB. Proliferation rates in TIB ranged 4.45 – 33.76 multiplies of biomass after two proliferation cycles. However, in general, the rainfed sugarcane varieties showed better response, growth, and regeneration rate than the irrigated sugarcane varieties. The in vitro-derived sugarcane seedlings have been successfully obtained from all varieties except for PS 881, with the highest shoots production was PSKA 942 (147.7 shoots/flask). [ABSTRACT FROM AUTHOR]