1. Small Noncoding RNAs in Cells Transformed by Human T-Cell Leukemia Virus Type 1: a Role for a tRNA Fragment as a Primer for Reverse Transcriptase.
- Author
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Ruggero, Katia, Guffanti, Alessandro, Corradin, Alberto, Sharma, Varun Kumar, De Bellis, Gianluca, Corti, Giorgio, Grassi, Angela, Zanovello, Paola, Bronte, Vincenzo, Ciminale, Vincenzo, and D'Agostino, Donna M.
- Subjects
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T-cell lymphoma , *HTLV-I , *TRANSFER RNA , *DNA primers , *REVERSE transcriptase - Abstract
The present study employed mass sequencing of small RNA libraries to identify the repertoire of small noncoding RNAs expressed in normal CD4+ T cells compared to cells transformed with human T-cell leukemia virus type 1 (HTLV-1), the causative agent of adult T-cell leukemia/lymphoma (ATLL). The results revealed distinct patterns of microRNA expression in HTLV-1- infected CD4+ T-cell lines with respect to their normal counterparts. In addition, a search for virus-encoded microRNAs yielded 2 sequences that originated from the plus strand of the HTLV-1 genome. Several sequences derived from tRNAs were expressed at substantial levels in both uninfected and infected cells. One of the most abundant tRNA fragments (tRF-3019) was derived from the 3= end of tRNA-proline. tRF-3019 exhibited perfect sequence complementarity to the primer binding site of HTLV-1. The results of an in vitro reverse transcriptase assay verified that tRF-3019 was capable of priming HTLV-1 reverse transcriptase. Both tRNA-proline and tRF-3019 were detected in virus particles isolated from HTLV-1-infected cells. These findings suggest that tRF-3019 may play an important role in priming HTLV-1 reverse transcription and could thus represent a novel target to control HTLV-1 infection. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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