1. A new quantitative RT-PCR method for sensitive detection of dengue virus in serum samples
- Author
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Sadon, Nadine, Delers, Anne, Jarman, Richard G., Klungthong, Chonticha, Nisalak, Ananda, Gibbons, Robert V., and Vassilev, Ventzislav
- Subjects
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BLOOD plasma , *DENGUE viruses , *DIAGNOSTIC use of polymerase chain reaction , *MOLECULAR diagnosis - Abstract
Abstract: In order to detect and identify dengue serotypes in serum samples, we developed a single-step quantitative reverse transcription-polymerase chain reaction (Q-RT-PCR) assay (referred to as Q-PCR). Sets of primers were selected from the capsid region of the viral genome. Dengue serotypes 1/3 and 2/4 were detected in two separate duplex amplification reactions using specific primers and fluorogenic TaqMan™ probes. Results obtained with this Q-PCR and the classical nested RT-PCR (N-PCR) assays were compared using a panel of 97 representative human sera collected from patients in Bangkok, Thailand. It is shown that the Q-PCR is a rapid, sensitive and reproducible tool for the detection and quantitation of the four dengue serotypes in clinical samples, and therefore of great interest for diagnostic use or for large cohort studies. [Copyright &y& Elsevier]
- Published
- 2008
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