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Background: Delayed graft function (DGF) after kidney transplantation is associated with adverse patients and allograft outcomes. A longer duration of DGF is predictive of worse graft outcomes compared to a shorter duration. Posttransplant serum β2‐microglobulin (B2M) is associated with long‐term graft outcomes, but its relationship with DGF recovery is unknown. Methods: We included all kidney‐only transplant recipients with DGF enrolled in the E‐DGF trial. Duration of DGF was defined as the interval between the transplant and the last dialysis session. We analyzed the association of standardized serum creatinine (Scr) and B2M on postoperative Days (POD) 1–7 during the subsequent days of DGF with the recovery of DGF. Results: A total of 97 recipients with DGF were included. The mean duration of DGF was 11.0 ± 11.2 days. Higher Scr was not associated with the duration of DGF in unadjusted or adjusted models. Higher standardized B2M, in contrast, was associated with a prolonged duration of DGF. This association remained in models adjusting for baseline characteristics from POD 2 (3.19 days longer, 95% CI: 0.46–5.93; p = 0.02) through Day 6 of DGF (4.97 days longer, 95% CI: 0.75–9.20; p = 0.02). There was minimal change in mean Scr (0.01 ± 0. 10 mg/dL per day; p = 0.32), while B2M significantly decreased as the time to recovery approached (–0.14 ± 0.05 mg/L per day; p = 0.006), among recipients with DGF. Conclusion: B2M is more strongly associated with DGF recovery than Scr. Posttransplant B2M may be an important biomarker to monitor during DGF. Trial Registration: ClinicalTrials.gov identifier: NCT 03864926 [ABSTRACT FROM AUTHOR]

Objective: To explore the detection effect and accuracy of scattering immunoassay and fluorescence immunochromatography for detection of serum β2-microglobulin. Methods: 88 patients with liver and kidney function injury admitted to our hospital from October 2016 to August 2017 were selected in this study. And the level of serum β2-microglobulin was detected by scattering immunoassay and fluorescence immunochromatography, respectively. The detection results, sensitivity, specificity and accuracy of serum mirncroglobulin were compared between the two methods Results: The detection rate by fluorescence immunochromatography was significantly higher than that of scattering immunoassay, while the missed detection rate was significantly lower than that of the scattering immune method (P<0.05). The sensitivity, specificity and diagnostic coincidence rate of fluorescence immunotomography were significantly higher than those of scattering immunotomography (P<0.05). The levels of serum β2-microglobulin, creatinine, uric acid and urea of these patients were significantly higher than normal (P<0.05). In terms of accuracy, the accuracy of fluorescence immunochromatography between and within batches was significantly higher than that of scattering immunoassay (P<0.05). Conclusion: The application of fluorescence immunochromatography for detection of serum β2-microglobulin in patients has a higher detection coincidence rate, sensitivity and accuracy, which can meet the requirements of clinical detection process, and is beneficial to the diagnosis and treatment of patients. Therefore, fluorescence immunochromatography should be further popularized in clinical application. [ABSTRACT FROM AUTHOR]